{"product_id":"t4-dna-ligase-ua070112","title":"T4 DNA Ligase","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e5 U\/μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eE.coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan style=\"color: rgb(38, 42, 51);font-size: 12px\"\u003e55.3 kDa\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e＞95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cb\u003eStorage Buffer:\u003c\/b\u003e\u003cspan style=\"color:#212529\"\u003e 20 mM Tris-HCl (pH 7.5), 50mM KCl, 1 mM DTT, 0.1 mM EDTA and 50% glycerol.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cb\u003e1 × Reaction Buffer:\u003c\/b\u003e 40 mM Tris-HCl \u003cspan style=\"color:#212529\"\u003e(pH 7.8\u003c\/span\u003e @ 25°C\u003cspan style=\"color:#212529\"\u003e)\u003c\/span\u003e, 10 mM MgCl2, 10 mMDTT, 0.5 mM ATP.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan style=\"color: rgb(38, 42, 51);font-size: 14px\"\u003e-20℃\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003eT4 DNA ligase catalyzes the formation of phosphodiester bonds between adjacent 5'-phosphate and 3'-hydroxyl ends in double-stranded DNA or RNA. This enzyme can repair single-strand nicks in double-stranded DNA, RNA, or DNA\/RNA hybrids. It can also join DNA fragments with sticky or blunt ends, but is inactive toward single-stranded nucleic acids. \u003cbr\u003eT4 DNA ligase requires ATP as a cofactor.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp style=\"margin:0cm -.7pt .0001pt 163.05pt;text-align:justify;text-indent:-127.05pt;text-justify:inter-ideograph;\"\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:10.0pt;layout-grid-mode:line;' lang=\"EN-US\"\u003e\u003cstrong\u003eApplication:\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"margin:0cm -.7pt .0001pt 163.05pt;text-align:justify;text-indent:-127.05pt;text-justify:inter-ideograph;\"\u003e\u003cspan lang=\"EN-US\"\u003eCloning of restriction enzyme generated DNA fragments.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"margin:0cm -.7pt .0001pt 163.05pt;text-align:justify;text-indent:-127.05pt;text-justify:inter-ideograph;\"\u003e\u003cspan lang=\"EN-US\"\u003eCloning of PCR products.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"margin:0cm -.7pt .0001pt 163.05pt;text-align:justify;text-indent:-127.05pt;text-justify:inter-ideograph;\"\u003e\u003cspan lang=\"EN-US\"\u003eJoining of double-stranded oligonucleotide linkers or adaptors to DNA.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"margin:0cm -.7pt .0001pt 163.05pt;text-align:justify;text-indent:-127.05pt;text-justify:inter-ideograph;\"\u003e\u003cspan lang=\"EN-US\"\u003eSite-directed mutagenesis.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"margin:0cm -.7pt .0001pt 163.05pt;text-align:justify;text-indent:-127.05pt;text-justify:inter-ideograph;\"\u003e\u003cspan lang=\"EN-US\"\u003eAmplified fragment length polymorphism (AFLP).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"margin:0cm -.7pt .0001pt 163.05pt;text-align:justify;text-indent:-127.05pt;text-justify:inter-ideograph;\"\u003e\u003cspan lang=\"EN-US\"\u003eLigase-mediated RNA detection.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"margin:0cm -.7pt .0001pt 163.05pt;text-align:justify;text-indent:-127.05pt;text-justify:inter-ideograph;\"\u003e\u003cspan lang=\"EN-US\"\u003eNick repair in duplex DNA, RNA or DNA\/RNA hybrids.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"margin:0cm -.7pt .0001pt 163.05pt;text-align:justify;text-indent:-127.05pt;text-justify:inter-ideograph;\"\u003e\u003cspan lang=\"EN-US\"\u003eSelf-circularization of linear DNA\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"margin:0cm -.7pt .0001pt 163.05pt;text-align:justify;text-indent:-127.6pt;text-justify:inter-ideograph;\"\u003e\u003cspan lang=\"EN-US\"\u003e\u003cstrong\u003eReaction Conditions:               \u003c\/strong\u003e1×T4 DNA Ligase Reaction Buffer. Incubate at 16 °C.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"margin:0cm -.7pt .0001pt 148.85pt;text-align:justify;text-indent:-112.85pt;text-justify:inter-ideograph;\"\u003e \u003c\/p\u003e\n\u003cp style=\"margin:0cm -.7pt .0001pt 163.05pt;text-align:justify;text-indent:-127.6pt;text-justify:inter-ideograph;\"\u003e\u003cspan lang=\"EN-US\"\u003e\u003cstrong\u003eInhibition and Inactivation:   \u003c\/strong\u003eT4 DNA Ligase is strongly inhibited by NaCl or KCl at concentrations more than 200 mM.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"margin-left:35.35pt;text-autospace:none;text-indent:-.05pt;\"\u003e\u003cspan lang=\"EN-US\"\u003e\u003cstrong\u003e                                                  \u003c\/strong\u003eInactivated by heating at 65 °C for 10 min or at 70 °C for 5 min.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eUnit Definition\u003c\/h4\u003e\u003cdiv\u003eOne Weiss unit of the enzyme catalyzes the conversion of 1 nmol of [32PPi] into Norit-adsorbable form in 20 min at 37°C. Enzyme activity is assayed in the following mixture: 66 mM Tris-HCl (pH 7.6), 6.6 mM MgCl2, 0.066 mM ATP, 10 mM DTT, 3.3 µM [32PPi].1 Weiss Unit = 200 CEUOne CEU is defined as the amount of enzyme required to give 50% ligation of HindIII fragments of lambda DNA in 30 min at 16°C.\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"1KU","offer_id":43085293781067,"sku":"UA070112-1KU","price":35.0,"currency_code":"USD","in_stock":true},{"title":"5KU","offer_id":43085293813835,"sku":"UA070112-5KU","price":130.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_c4ba0e03-7910-43dd-b715-2c1b550c6f86.png?v=1788001307","url":"https:\/\/www.antbioinc.com\/products\/t4-dna-ligase-ua070112","provider":"AntBio","version":"1.0","type":"link"}