{"product_id":"t-ua090053","title":"人T细胞激活试剂盒（磁珠）","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e \u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReconstitution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan style=\"color: rgb(73, 80, 87);font-size: 13px\"\u003eIL-2:  \u003c\/span\u003e\u003cspan style=\"color: rgb(51, 51, 51);font-size: 14px\"\u003eReconstitute at 0.1-1 mg\/ml according to the size in ultrapure water after rapid centrifugation.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003ctable class=\"table table-bordered\" style=\"width: 1191.93px; margin-left: 1.67128px;\"\u003e \u003ctbody\u003e\n\u003ctr\u003e  \u003ctd style=\"width: 594.755px;\"\u003e  \u003cp\u003e\u003cspan style=\"font-size:10.0pt\"\u003e\u003cb\u003e\u003c\/b\u003eT Cell Activation Nanobeads，Human（RUO）\u003cb\u003e\u003c\/b\u003e\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd style=\"width: 596.435px;\"\u003e  \u003cp\u003e\u003cspan style=\"font-size:10.0pt\"\u003eKeep away from light2-8℃，Do not freeze\u003cb\u003e\u003c\/b\u003e\u003c\/span\u003e\u003c\/p\u003e  \u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e  \u003ctd style=\"width: 596.435px;\"\u003e  \u003cp\u003eIL-2 Protein Human \u003cbr\u003e\u003c\/p\u003e  \u003c\/td\u003e  \u003ctd style=\"width: 596.435px;\"\u003e  \u003cp\u003e\u003cspan style=\"font-size:  10.0pt;color:#333333\"\u003e12 months from date of  receipt, lyophilized powder stored at -20 to -80\u003c\/span\u003e\u003cspan style=\"font-size:10.0pt;color:#333333\"\u003e℃.\u003c\/span\u003e\u003c\/p\u003e  \u003cp\u003e\u003cspan style=\"font-size:10.0pt;color:#333333\"\u003e3 months, -20 to -80℃ under sterile  conditions after reconstitution.\u003c\/span\u003e\u003c\/p\u003e  \u003cp\u003e\u003cspan style=\"font-size:10.0pt;color:#333333\"\u003e1 week, 2 to 8℃ under sterile conditions  after reconstitution.\u003c\/span\u003e\u003c\/p\u003e  \u003cp\u003e\u003cspan style=\"font-size:10.0pt;color:#333333\"\u003e· Please avoid repeated freeze-thaw cycles.\u003c\/span\u003e\u003c\/p\u003e  \u003cp\u003e\u003cb\u003e\u003cspan style=\"font-size:10.0pt\"\u003e \u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e  \u003c\/td\u003e \u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:none;margin-left:36.0pt;\" border=\"1\" cellspacing=\"0\" cellpadding=\"0\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height:28.6pt;\"\u003e\n\u003ctd style=\"border:solid black 1.0pt;height:28.6pt;padding:0cm 0cm 0cm 0cm;width:131.7pt;\" width=\"176\" valign=\"top\"\u003e\u003cp class=\"TableParagraph\" style=\"margin-top:6.9pt;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003eContain\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid black 1.0pt;height:28.6pt;padding:0cm 0cm 0cm 0cm;width:78.35pt;\" width=\"104\" valign=\"top\"\u003e\u003cp class=\"TableParagraph\" style=\"margin-top:6.9pt;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003eFormulation\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid black 1.0pt;height:28.6pt;padding:0cm 0cm 0cm 0cm;width:88.1pt;\" width=\"117\" valign=\"top\"\u003e\n\u003cp class=\"TableParagraph\" style=\"line-height:13.8pt;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003eReference\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"TableParagraph\" style=\"line-height:14.35pt;margin-top:1.35pt;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003edosage\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid black 1.0pt;height:28.6pt;padding:0cm 0cm 0cm 0cm;width:88.05pt;\" width=\"117\" valign=\"top\"\u003e\u003cp class=\"TableParagraph\" style=\"line-height:14.35pt;margin:1.35pt 0cm .0001pt 5.55pt;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003eFor 10^8cells\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid black 1.0pt;height:28.6pt;padding:0cm 0cm 0cm 0cm;width:62.3pt;\" width=\"83\" valign=\"top\"\u003e\u003cp class=\"TableParagraph\" style=\"line-height:14.35pt;margin:1.35pt 0cm .0001pt 5.55pt;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003eFor 4*10^8cells\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:28.6pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid black 1.0pt;height:28.6pt;padding:0cm 0cm 0cm 0cm;width:131.7pt;\" width=\"176\" valign=\"top\"\u003e\n\u003cp class=\"TableParagraph\" style=\"line-height:13.8pt;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003eIL-2 Protein,\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"TableParagraph\" style=\"line-height:14.35pt;margin-top:1.35pt;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003eHuman\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:28.6pt;padding:0cm 0cm 0cm 0cm;width:78.35pt;\" width=\"104\" valign=\"top\"\u003e\n\u003cp class=\"TableParagraph\" style=\"line-height:13.8pt;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003eLyophilized\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"TableParagraph\" style=\"line-height:14.35pt;margin-top:1.35pt;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003ePowder\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:28.6pt;padding:0cm 0cm 0cm 0cm;width:88.1pt;\" width=\"117\" valign=\"top\"\u003e\u003cp class=\"TableParagraph\" style=\"margin-top:6.9pt;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003e10 ng\/mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:28.6pt;padding:0cm 0cm 0cm 0cm;width:88.05pt;\" width=\"117\" valign=\"top\"\u003e\u003cp class=\"TableParagraph\" style=\"margin:6.9pt 0cm .0001pt 5.55pt;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003e5\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;'\u003eμ\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003eg\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:28.6pt;padding:0cm 0cm 0cm 0cm;width:62.3pt;\" width=\"83\" valign=\"top\"\u003e\u003cp class=\"TableParagraph\" style=\"margin:6.9pt 0cm .0001pt 5.55pt;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003e10\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;'\u003eμ\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003eg\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:43.25pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid black 1.0pt;height:43.25pt;padding:0cm 0cm 0cm 0cm;width:131.7pt;\" width=\"176\"\u003e\n\u003cp class=\"TableParagraph\" style=\"line-height:13.8pt;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003eT Cell Activation Nanobeads，Human（RUO）\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"TableParagraph\" style=\"line-height:14.35pt;margin:1.35pt 0cm .0001pt;\"\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:43.25pt;padding:0cm 0cm 0cm 0cm;width:78.35pt;\" width=\"104\" valign=\"top\"\u003e\u003cp class=\"TableParagraph\" style=\"margin-top:6.9pt;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003eLiquid\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:43.25pt;padding:0cm 0cm 0cm 0cm;width:88.1pt;\" width=\"117\" valign=\"top\"\u003e\u003cp class=\"TableParagraph\" style=\"margin-top:6.9pt;\"\u003e\u003cspan style=\"background-color:white;color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003e5 μL\/\u003c\/span\u003e\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003e1×10\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;position:relative;top:-4.0pt;' lang=\"EN-US\"\u003e\u003csup\u003e6\u003c\/sup\u003e\u003c\/span\u003e\u003cspan style=\"background-color:white;color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003ecells\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:43.25pt;padding:0cm 0cm 0cm 0cm;width:88.05pt;\" width=\"117\" valign=\"top\"\u003e\u003cp class=\"TableParagraph\" style=\"margin:6.9pt 0cm .0001pt 5.55pt;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003e0.5mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:43.25pt;padding:0cm 0cm 0cm 0cm;width:62.3pt;\" width=\"83\" valign=\"top\"\u003e\u003cp class=\"TableParagraph\" style=\"margin:6.9pt 0cm .0001pt 5.55pt;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;' lang=\"EN-US\"\u003e2mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan lang=\"EN-US\"\u003eComplete Medium Formulation for Reference:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;width:202.8pt;\" border=\"0\" cellspacing=\"0\" cellpadding=\"0\" width=\"270\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height:15.5pt;\"\u003e\n\u003ctd style=\"background-color:white;border:solid black 1.0pt;height:15.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:122.65pt;\" width=\"164\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:#0F1115;\"\u003e\u003cspan style=\"font-family:宋体;font-size:12.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:white;border-left:none;border:solid black 1.0pt;height:15.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:80.15pt;\" width=\"107\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:#0F1115;\"\u003e\u003cspan style=\"font-family:宋体;font-size:12.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003eFinal Concentration\u003c\/strong\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:15.5pt;\"\u003e\n\u003ctd style=\"background-color:white;border-top:none;border:solid black 1.0pt;height:15.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:122.65pt;\" width=\"164\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:#0F1115;\"\u003e\u003cspan style='font-family:\"var(--dsw-font-markdown-table)\",serif;font-size:12.0pt;' lang=\"EN-US\"\u003eRPMI-1640\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:white;border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:15.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:80.15pt;\" width=\"107\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:#0F1115;\"\u003e\u003cspan style='font-family:\"var(--dsw-font-markdown-table)\",serif;font-size:12.0pt;' lang=\"EN-US\"\u003e90%\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:15.5pt;\"\u003e\n\u003ctd style=\"background-color:white;border-top:none;border:solid black 1.0pt;height:15.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:122.65pt;\" width=\"164\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:#0F1115;\"\u003e\u003cspan style='font-family:\"var(--dsw-font-markdown-table)\",serif;font-size:12.0pt;' lang=\"EN-US\"\u003eFetal Bovine Serum\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:white;border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:15.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:80.15pt;\" width=\"107\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:#0F1115;\"\u003e\u003cspan style='font-family:\"var(--dsw-font-markdown-table)\",serif;font-size:12.0pt;' lang=\"EN-US\"\u003e10%\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:15.5pt;\"\u003e\n\u003ctd style=\"background-color:white;border-top:none;border:solid black 1.0pt;height:15.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:122.65pt;\" width=\"164\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:#0F1115;\"\u003e\u003cspan style='font-family:\"var(--dsw-font-markdown-table)\",serif;font-size:12.0pt;' lang=\"EN-US\"\u003eSodium Pyruvate 100×\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:white;border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:15.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:80.15pt;\" width=\"107\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:#0F1115;\"\u003e\u003cspan style='font-family:\"var(--dsw-font-markdown-table)\",serif;font-size:12.0pt;' lang=\"EN-US\"\u003e1×\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:15.5pt;\"\u003e\n\u003ctd style=\"background-color:white;border-top:none;border:solid black 1.0pt;height:15.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:122.65pt;\" width=\"164\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:#0F1115;\"\u003e\u003cspan style='font-family:\"var(--dsw-font-markdown-table)\",serif;font-size:12.0pt;' lang=\"EN-US\"\u003eL-Glutamine 100×\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:white;border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:15.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:80.15pt;\" width=\"107\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:#0F1115;\"\u003e\u003cspan style='font-family:\"var(--dsw-font-markdown-table)\",serif;font-size:12.0pt;' lang=\"EN-US\"\u003e1×\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:15.5pt;\"\u003e\n\u003ctd style=\"background-color:white;border-top:none;border:solid black 1.0pt;height:15.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:122.65pt;\" width=\"164\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:#0F1115;\"\u003e\u003cspan style='font-family:\"var(--dsw-font-markdown-table)\",serif;font-size:12.0pt;' lang=\"EN-US\"\u003eHEPES\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:white;border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:15.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:80.15pt;\" width=\"107\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:#0F1115;\"\u003e\u003cspan style='font-family:\"var(--dsw-font-markdown-table)\",serif;font-size:12.0pt;' lang=\"EN-US\"\u003e1×\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:15.5pt;\"\u003e\n\u003ctd style=\"background-color:white;border-top:none;border:solid black 1.0pt;height:15.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:122.65pt;\" width=\"164\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:#0F1115;\"\u003e\u003cspan style='font-family:\"var(--dsw-font-markdown-table)\",serif;font-size:12.0pt;' lang=\"EN-US\"\u003ePenicillin-Streptomycin\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:white;border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:15.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:80.15pt;\" width=\"107\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:#0F1115;\"\u003e\u003cspan style='font-family:\"var(--dsw-font-markdown-table)\",serif;font-size:12.0pt;' lang=\"EN-US\"\u003e1×\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:15.5pt;\"\u003e\n\u003ctd style=\"background-color:white;border-top:none;border:solid black 1.0pt;height:15.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:122.65pt;\" width=\"164\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:#0F1115;\"\u003e\u003cspan style='font-family:\"var(--dsw-font-markdown-table)\",serif;font-size:12.0pt;' lang=\"EN-US\"\u003eβ-Mercaptoethanol\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:white;border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:15.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:80.15pt;\" width=\"107\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:#0F1115;\"\u003e\u003cspan style='font-family:\"var(--dsw-font-markdown-table)\",serif;font-size:12.0pt;' lang=\"EN-US\"\u003e55 μM\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan lang=\"EN-US\"\u003eProcedure\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan lang=\"EN-US\"\u003e1. Prepare a single\u003c\/span\u003e\u003cspan style='font-family:\"MS Gothic\";'\u003e‑\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003ecell suspension of human peripheral blood mononuclear cells (PBMCs). Perform cell counting using AO\/PI to determine the cell number required. Resuspend 1×10\u003c\/span\u003e\u003cspan style='font-family:\"MS Gothic\";'\u003e⁶\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e cells in 995 \u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003eL of complete T\u003c\/span\u003e\u003cspan style='font-family:\"MS Gothic\";'\u003e‑\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003ecell medium. For T\u003c\/span\u003e\u003cspan style='font-family:\"MS Gothic\";'\u003e‑\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003ecell activation, the recommended concentration is (1\u003c\/span\u003e\u003cspan\u003e–\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e3)\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e10\u003c\/span\u003e\u003cspan style='font-family:\"MS Gothic\";'\u003e⁶\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e cells\/mL. For a 24\u003c\/span\u003e\u003cspan style='font-family:\"MS Gothic\";'\u003e‑\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003ewell plate, a concentration of 1×10\u003c\/span\u003e\u003cspan style='font-family:\"MS Gothic\";'\u003e⁶\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e cells\/mL is recommended.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan lang=\"EN-US\"\u003e2. Add the following cytokines and beads to the cell suspension from step 1:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan lang=\"EN-US\"\u003e   - Add 5 μL of T Cell Activation Nanobeads per 1×10\u003c\/span\u003e\u003cspan style='font-family:\"MS Gothic\";'\u003e⁶\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e cells and mix thoroughly but gently.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan lang=\"EN-US\"\u003e   - Add 10 ng\/mL human interleukin\u003c\/span\u003e\u003cspan style='font-family:\"MS Gothic\";'\u003e‑\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e2 (IL\u003c\/span\u003e\u003cspan style='font-family:\"MS Gothic\";'\u003e‑\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e2) protein.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan lang=\"EN-US\"\u003e3. Transfer the cells to a 24\u003c\/span\u003e\u003cspan style='font-family:\"MS Gothic\";'\u003e‑\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003ewell plate and incubate statically in a 37\u003c\/span\u003e\u003cspan style='font-family:\"Times New Roman\",serif;' lang=\"EN-US\"\u003e \u003c\/span\u003e\u003cspan\u003e°\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003eC, 5\u003c\/span\u003e\u003cspan style='font-family:\"Times New Roman\",serif;' lang=\"EN-US\"\u003e \u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e% CO\u003c\/span\u003e\u003cspan style='font-family:\"Times New Roman\",serif;' lang=\"EN-US\"\u003e₂\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e incubator for 48\u003c\/span\u003e\u003cspan style='font-family:\"Times New Roman\",serif;' lang=\"EN-US\"\u003e \u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003eh.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan lang=\"EN-US\"\u003e4. After 48\u003c\/span\u003e\u003cspan style='font-family:\"Times New Roman\",serif;' lang=\"EN-US\"\u003e \u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003eh of culture, check cell health and record images.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan lang=\"EN-US\"\u003e5. Centrifuge the cell suspension at 400×g for 5 minutes and discard the supernatant. Wash the cell pellet with PBS.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan lang=\"EN-US\"\u003e6. Centrifuge the cell suspension at 400×g for 5 minutes and discard the supernatant. Resuspend the cells in 0.5\u003c\/span\u003e\u003cspan style='font-family:\"Times New Roman\",serif;' lang=\"EN-US\"\u003e \u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003emL of 1\u003c\/span\u003e\u003cspan style='font-family:\"Times New Roman\",serif;' lang=\"EN-US\"\u003e \u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e% bovine serum albumin (BSA) and count the cells. Assess cell viability, which should be \u0026gt;95\u003c\/span\u003e\u003cspan style='font-family:\"Times New Roman\",serif;' lang=\"EN-US\"\u003e \u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e%.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan lang=\"EN-US\"\u003e7. Block 1×10\u003c\/span\u003e\u003cspan style='font-family:\"MS Gothic\";'\u003e⁶\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e cells with 10\u003c\/span\u003e\u003cspan style='font-family:\"Times New Roman\",serif;' lang=\"EN-US\"\u003e \u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003eµg of human IgG antibody. Incubate on ice for 30 minutes.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan lang=\"EN-US\"\u003e8. According to the manufacturer’s instructions, add Brilliant Violet 421™ anti\u003c\/span\u003e\u003cspan style='font-family:\"MS Gothic\";'\u003e‑\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003ehuman CD25 antibody and Alexa Fluor® 488 anti\u003c\/span\u003e\u003cspan style='font-family:\"MS Gothic\";'\u003e‑\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003ehuman CD69 antibody, and incubate at 4\u003c\/span\u003e\u003cspan style='font-family:\"Times New Roman\",serif;' lang=\"EN-US\"\u003e \u003c\/span\u003e\u003cspan\u003e°\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003eC for 30 minutes.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan lang=\"EN-US\"\u003e9. Centrifuge the cell suspension at 400×g for 5 minutes and wash the cell pellet twice with PBS containing 1\u003c\/span\u003e\u003cspan style='font-family:\"Times New Roman\",serif;' lang=\"EN-US\"\u003e \u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e% BSA.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan lang=\"EN-US\"\u003e10. Resuspend the cell pellet in 200\u003c\/span\u003e\u003cspan style='font-family:\"Times New Roman\",serif;' lang=\"EN-US\"\u003e \u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003eµL of PBS for flow cytometric analysis (\u003c\/span\u003e\u003cspan\u003e≥\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e10,000 cells are required).\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eGuidelines\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e1. T Cell Activation Nanobeads, Human (RUO) should be thoroughly mixed before use. During the operation, efforts should be made to minimize bubble formation to avoid any impact on subsequent operations.\u003cbr\u003e2. All operations throughout the process should be conducted in a sterile environment.\u003cbr\u003e3. For the initial activation, it is necessary to ensure that the reagents and cells remain in contact for 2 to 3 days. Removing the reagents too early will result in insufficient T cell activation and decreased proliferation ability.\u003cbr\u003e4. T cells can undergo virus transduction experiments 2 days after activation. It is necessary to independently explore the optimal transduction titer and timing.\u003cbr\u003e5. The residual EDTA in the cell system will significantly inhibit T cell activation stimulation. Before the experiment, it is necessary to thoroughly remove it.\u003cbr\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"For 10E8cells","offer_id":43038354636875,"sku":"UA090053-For 10E8cells","price":300.0,"currency_code":"USD","in_stock":true},{"title":"For 4*10E8cells","offer_id":43038354669643,"sku":"UA090053-For 4*10E8cells","price":900.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/e4b54ba0-6b09-48bf-a0f8-ccbefc22fb98_7c2f7a57-e962-4a12-a20f-4630badb827e.jpg?v=1786701608","url":"https:\/\/www.antbioinc.com\/products\/t-ua090053","provider":"AntBio","version":"1.0","type":"link"}