{"product_id":"sting-knockdown-hela-cell-lysate-s0y0029","title":"STING-Knockdown HeLa Cell Lysate","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eWB\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e2 mg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eSupplied in SDS Sample Buffer: 50 mM Tris-HCl (pH 6.8 at 25°C), 2% w\/v SDS, 10% glycerol, 1% v\/v β-Mercaptoethanol, 0.01% w\/v bromophenol blue or phenol red.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-20 °C for 1 months under sterile conditions; \u003cbr\u003e-80 °C for 24 months under sterile conditions.\u003cbr\u003ePlease avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan\u003eStimulator of Interferon Genes (STING, encoded by STING1 \/ TMEM173) is an ER-resident transmembrane adaptor essential for innate immune sensing of cytosolic nucleic acids. Downstream of cyclic GMP-AMP synthase (cGAS), STING binds the cyclic dinucleotide 2'3'-cGAMP, triggering its translocation to the Golgi apparatus and subsequent recruitment and activation of TBK1 and IKK. This signaling cascade phosphorylates transcription factors IRF3 and NF-κB, driving the robust expression of type I interferons (IFN-α\/\u003c\/span\u003e\u003cspan\u003eβ\u003c\/span\u003e\u003cspan\u003e), pro-inflammatory cytokines, and interferon-stimulated genes (ISGs). STING also participates in non-canonical autophagy and cellular senescence programs. STING antibodies are widely utilized in Western blotting, immunofluorescence, and immunohistochemistry to monitor pathway activation and innate immune responses. STING knockout (KO) abolishes cytosolic DNA-driven innate immune signaling, blunting type I interferon induction upon pathogen infection or genomic stress, which dampens antitumor immune surveillance while protecting cells against pathological autoinflammation.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1kit","offer_id":48961592164427,"sku":"S0Y0029-1kit","price":670.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/cd0ed34d92254f89a2f1a9f2ccb6e9b8.jpg?v=1790676090","url":"https:\/\/www.antbioinc.com\/products\/sting-knockdown-hela-cell-lysate-s0y0029","provider":"AntBio","version":"1.0","type":"link"}