{"product_id":"steap1-flaghis-tag-full-length-protein-human-detergent-ua060043","title":"STEAP1 Flag\u0026His Tag Full Length Protein, Human (Detergent)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eSTEAP1, STEAP1 protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAccession\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eQ9UHE8\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAmino Acid Sequence\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eMet1–Leu399 with a Flag Tag at the N-terminus and a His Tag at the C-terminus.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHEK293\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e30-40 kDa (Reducing)\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHis Tag, Flag Tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e25 mM Hepes, pH 7.5, 150 mM NaCl, 0.006% GDN, 5% glycerol \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eStable for 3 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e1. Kwangsoo K, Sharmistha M, Gang W, Vladimir B, Jinmei S, Ye Y, Sebastien P, Da-Neng W, Ah-Lim T, Ming Z; Six-Transmembrane Epithelial Antigen of Prostate 1 (STEAP1) Has a Single b Heme and Is Capable of Reducing Metal Ion Complexes and Oxygen. Biochemistry. 2016 Dec 6; 55(48): 6673–6684.\u003cbr\u003e2. Oosterheert W, van Bezouwen LS, Rodenburg RNP, Granneman J, Förster F, Mattevi A, Gros P. Cryo-EM structures of human STEAP4 reveal mechanism of iron (III) reduction. Nat Commun. 2018 Oct 18;9(1):4337. \u003cbr\u003e3. Kehan C, Lie W, Jiemin S, Ah-Lim T, Ming Z, Gang W. Mechanism of stepwise electron transfer in six-transmembrane epithelial antigen of the prostate (STEAP) 1 and 2. eLife. 2023 Nov 20; 12:RP88299.\u003cbr\u003e4. Oosterheert W, Gros P. Cryo-electron microscopy structure and potential enzymatic function of human six-transmembrane epithelial antigen of the prostate 1 (STEAP1). J Biol Chem. 2020 Jul 10;295(28):9502-9512.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003eSTEAP1 is a member of the STEAP family and is highly expressed in multiple cancers, including prostate, bladder, colorectal, lung, ovarian, and breast cancers and Ewing sarcoma, with limited expression in normal tissues, making it an attractive diagnostic and therapeutic target. Structurally, STEAP1 is a six-transmembrane protein that assembles into homotrimers, with each protomer binding a single b-type heme coordinated by conserved histidines. Unlike STEAP2–STEAP4, STEAP1 lacks an N-terminal cytosolic oxidoreductase domain (OxRD) and therefore does not independently support NADPH-dependent ferric reductase activity in cells. Nevertheless, its transmembrane domain is redox-competent: reduced STEAP1 can reduce Fe³⁺\/Cu²⁺ complexes and O₂, and STEAP1 can accept electrons from reduced FAD or cytochrome b₅ reductase\/NADH. STEAP1 also forms heterotrimers with STEAP2 and may utilize reduced FAD generated by STEAP2. Fusion of the STEAP1 transmembrane domain to the STEAP4 OxRD restores cellular ferric reductase activity, indicating that STEAP1 functions as a transmembrane electron-transfer module requiring an external electron-donating partner.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eTherapeutically, STEAP1 is targeted by the monoclonal antibody mAb120.545 and its humanized derivatives.\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eClinically, humanized mAb120.545 variants have been developed as antibody–drug conjugates (e.g., DSTP3086S\/vandortuzumab vedotin) and as ⁸⁹Zr-labeled immuno-PET agents (e.g., MSTP2109A) for prostate cancer imaging. In addition, STEAP1-derived peptides are immunogenic and can be recognized by cytotoxic T lymphocytes, supporting STEAP1 as a candidate for vaccine-based immunotherapy.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"25μg","offer_id":67396870471755,"sku":"UA060043-25μg","price":700.0,"currency_code":"USD","in_stock":true},{"title":"100μg","offer_id":67396870504523,"sku":"UA060043-100μg","price":2165.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/58b1fd463df44fc0a5acfddff581c13f_cb82c039-d76e-4ef1-9ce1-7e666f874587.jpg?v=1793840471","url":"https:\/\/www.antbioinc.com\/products\/steap1-flaghis-tag-full-length-protein-human-detergent-ua060043","provider":"AntBio","version":"1.0","type":"link"}