{"product_id":"sgc1sgc1-gst-tag-protein-human-ua085109","title":"sGCα1\/sGCβ1 GST Tag Protein, Human","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAccession\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003esGCα1: Q02108、 sGCβ1: Q02153\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAmino Acid Sequence\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003esGCα1: Met1-Asp690 with a GST tag at the N-terminus;\u003cbr\u003esGCβ1: Met1-Asp619;\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eBaculovirus-InsectCells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e70-100kDa (Reducing)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eGST Tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003ePBS, pH 7.4\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eStable for 12 months upon stored at -80\u003c\/span\u003e\u003cspan\u003e℃\u003c\/span\u003e\u003cspan\u003e from the date of receipt. And avoid repeated freeze-thaws cycles.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e1. Giuili G, Scholl U, Bulle F, Guellaën G. Molecular cloning of the cDNAs coding for the two subunits of soluble guanylyl cyclase from human brain. FEBS Lett. 1992 Jun 8;304(1):83-8.\u003cbr\u003e2. Allerston CK, von Delft F, Gileadi O. Crystal structures of the catalytic domain of human soluble guanylate cyclase. PLoS One. 2013;8(3):e57644.\u003cbr\u003e3. Seeger F, Quintyn R, Tanimoto A, Williams GJ, Tainer JA, Wysocki VH, Garcin ED. Interfacial residues promote an optimal alignment of the catalytic center in human soluble guanylate cyclase: heterodimerization is required but not sufficient for activity. Biochemistry. 2014 Apr 8;53(13):2153-65.\u003cbr\u003e4. Zabel U, Weeger M, La M, Schmidt HH. Human soluble guanylate cyclase: functional expression and revised isoenzyme family. Biochem J. 1998 Oct 1;335 (Pt 1) (Pt 1):51-7.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan\u003esGCα1\/β1 (Soluble Guanylate Cyclase α1\/β1 Heterodimer), encoded by the GUCY1A1 and GUCY1B1 genes, is a heterodimeric heme-containing metalloprotein belonging to the soluble guanylate cyclase family. The GUCY1A1–GUCY1B1 heterodimer is the predominant and functionally dominant isoform of sGC in most tissues. It serves as the primary intracellular receptor for nitric oxide (NO), the endothelium-derived gasotransmitter central to cardiovascular homeostasis. The sGCα1\/β1 complex does not exist as a functional NO sensor in the absence of its heme prosthetic group. Maturation requires the chaperone Hsp90, which associates with the heme-free (apo) β1 subunit and drives ferrous heme insertion; this event triggers Hsp90 dissociation and permits β1 to bind α1, yielding the mature, NO-responsive heterodimer. NO binds to the ferrous heme within the β1 subunit’s H-NOX domain, breaking the axial Fe–His105 bond and initiating a “closed-to-open” transition in the heme pocket. This local change propagates through the PAS and coiled-coil domains, straightening the coiled-coil helices and driving the catalytic domain into a closed, active conformation. Activated sGCα1\/β1 catalyzes the conversion of GTP to the second messenger cGMP, which in turn activates cGMP-dependent protein kinase (PKG); PKG phosphorylates downstream substrates and also feeds back to phosphorylate sGCα1 at Ser64, establishing a negative feedback loop. The α1β1 heterodimer is highly expressed in the cardiovascular system, platelets, and brain, where it mediates vasodilation, inhibition of platelet aggregation, and neuronal signaling. Disruption of sGCα1\/β1 function is pathophysiologically linked to hypertension—male sGCα1-knockout mice develop spontaneous hypertension—and to pulmonary arterial hypertension, in which reduced sGC expression and impaired α1β1 heterodimer formation contribute to NO–sGC–cGMP signaling dysfunction.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"10μg","offer_id":67471319302219,"sku":"UA085109-10μg","price":665.0,"currency_code":"USD","in_stock":true},{"title":"50μg","offer_id":67471319334987,"sku":"UA085109-50μg","price":2655.0,"currency_code":"USD","in_stock":true},{"title":"100μg","offer_id":67471319367755,"sku":"UA085109-100μg","price":3970.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_2c8acbc7-e3c5-4a0b-88c4-7bea617db050.png?v=1794564109","url":"https:\/\/www.antbioinc.com\/products\/sgc1sgc1-gst-tag-protein-human-ua085109","provider":"AntBio","version":"1.0","type":"link"}