{"product_id":"s0d0048","title":"重组核心链霉亲和素","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003eLyophilized powder (5 mM PB, pH 7.4) or\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eLyophilized powder (20 mM HEPES buffer, trehalose, TCEP, pH 7.0)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e四聚体54.0kDa，单体13.5kDa\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e≥95%（SDS-PAGE检测）\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore at -20℃\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003eDue to the inclusion of stabilizers and other excipients during the lyophilization process, we recommend first using an internal assay method to accurately quantify the protein content in the product before proceeding with subsequent experiments, and adjusting the dosage as needed based on actual conditions. We suggest prioritizing the UV absorption method for quantification, as follows:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAfter removing the sample from low-temperature storage, allow it to equilibrate to room temperature and wipe off any condensation on the outside of the vial. Carefully open the cap to prevent loss of lyophilized powder due to splashing. Reconstitute with deionized water or buffer, ensure complete dissolution, then dilute to an appropriate concentration. Gently transfer the solution into a cuvette, using deionized water or buffer as the blank control, and measure the absorbance at 280 nm (absorbance is recommended to be maintained within the range of 0.1–1.5). Substitute this value into the following formula to calculate the protein concentration:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eC = N × A₂₈₀nm \/ E(0.1% at 280 nm)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eIn the formula: C: protein mass concentration of the sample stock solution (mg\/mL)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eA280nm: absorbance of the protein solution measured at 280 nm\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eN: dilution factor\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eE(0.1% at 280nm): extinction coefficient of a 1 mg\/mL protein solution\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eFor example, after fully dissolving and mixing the lyophilized powder of this product, take an appropriate amount of the stock solution, dilute it 10-fold, and measure its absorbance at 280 nm to be 0.933; then the protein concentration of the stock solution is 10 × 0.933 \/ 3.11 = 3 mg\/mL.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003ePrecautions:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1. The recombinant core streptavidin lyophilized powder is easily soluble in water, with a solubility reaching 10 mg\/mL or higher;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2. It is recommended to use purified water for dissolution;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3. If there are undissolved substances, it is recommended to extend the reconstitution time. Alternatively, insoluble particles can be removed by centrifugation or other methods before use in subsequent experiments, which will not significantly affect the total protein concentration.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003e4. Given the unique properties of proteins, it is advisable to prepare lyophilized powder immediately before use. After dissolution, repeated freeze-thaw cycles should be avoided. If multiple uses are required, it is recommended to aliquot and store at ≤-20℃ (preferably for no more than one year); avoid long-term storage at 4℃ (for ≤7 days).\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"10mg","offer_id":43031150624843,"sku":"S0D0048-10mg","price":0.1,"currency_code":"USD","in_stock":true},{"title":"100mg","offer_id":43031150657611,"sku":"S0D0048-100mg","price":0.1,"currency_code":"USD","in_stock":true},{"title":"1g","offer_id":43031150690379,"sku":"S0D0048-1g","price":0.1,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_733e2a44-8a54-4223-a902-a6fcb7201def.png?v=1786528850","url":"https:\/\/www.antbioinc.com\/products\/s0d0048","provider":"AntBio","version":"1.0","type":"link"}