{"product_id":"receptor-beads-ua086151","title":"Receptor beads","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e1 mL \/瓶\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStorage: Store at 2–8°C, protected from light. Do not freeze. During transport, it is recommended to use ice packs, avoiding direct contact between the ice and the microspheres. Shelf life: 3 years.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003eThis product is a receptor microsphere specifically designed for homogeneous chemiluminescence systems. It is prepared using internally dyed polystyrene microspheres, offering stable performance, high luminescence efficiency, and excellent monodispersity. It can be used in pairs with oxygen-supplying microspheres (donor microspheres) to generate a specific light signal at 610 nm upon singlet oxygen activation, enabling wash-free, homogeneous, and highly sensitive biomolecular detection. It is suitable for the development of homogeneous immunoassays and diagnostic reagents targeting antigens\/antibodies, small molecules, nucleic acids, and more.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• Particle size: 200 nm\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• Surface functional group: Carboxyl (COOH)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• Solid content: 1.0%\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• Microsphere density: 1.05 g\/cm³\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• Appearance: Off-white suspension\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• Storage medium: Deionized water (containing trace amounts of surfactant)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e.   Refractive index: 1.59 (589 nm, 25°C)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e.   Carboxyl group content: 80–120 μmol\/g\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003eRecommended Coupling Procedure (Taking Antibody Conjugation as an Example)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1. Reagent Preparation\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e. Activation Buffer: 50 mM MES, 50 mM NaCl, pH 6.0 ± 0.05\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e. EDC Solution: 10 mg\/mL (prepared in activation buffer, use immediately after preparation)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e. NHS Solution: 10 mg\/mL (prepared in activation buffer, use immediately after preparation)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e. Labeling Buffer: 40 mM borate buffer, pH 7.8 ± 0.05\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e. Washing Buffer: 0.5% Triton X-405, 0.1% Tween-20\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e. Blocking Buffer: 10% BSA, 7.5% glycine, pH 8.0\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e. Storage Buffer: Homogeneous Luminescence–Specific Diluent\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2. Microsphere Activation\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1). Take 0.1 mL of receptor microspheres (1.0%), add 1 mL of activation buffer, and sonicate to mix thoroughly.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2). Centrifuge at 15°C and 20,000 g for 15–20 minutes, then discard the supernatant.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3). Repeat washing once, discarding the supernatant.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e4). Resuspend in 1 mL of activation buffer, add EDC and NHS, and sonicate to mix.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e5). Incubate in the dark at 25°C and 40 r\/min for 15–20 minutes.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e6). Centrifuge to discard the supernatant, and wash twice with activation buffer.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3. Antibody Conjugation\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e. Resuspend the microspheres in labeling buffer.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e. Add the target antibody and mix well.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e. 25℃, 40 r\/min, protect from light, couple for 2 h\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e4. Blocking and Storage\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e. Add blocking solution, incubate at 25℃, 40 r\/min, protect from light, block for 2 h\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e. Wash 3–4 times by centrifugation with wash buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e. Finally resuspend in homogeneous luminescence diluent\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e. Store at 2–8℃, protected from light, for later use\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eGuidelines\u003c\/h4\u003e\u003cdiv\u003e.   Perform all operations under light-protected conditions to avoid signal attenuation caused by illumination.\u003cbr\u003e.   Freezing is strictly prohibited, as it can cause microsphere aggregation and loss of function.\u003cbr\u003e.   Before use, shake thoroughly or sonicate for dispersion; minor aggregates can be eliminated by sonication.\u003cbr\u003e.   After microsphere activation, coupling is recommended immediately; prolonged storage is not advised.\u003cbr\u003e.   Avoid the introduction of polyvalent cations, organic solvents, and strong acids or bases into the system.\u003cbr\u003e.   For research and in vitro diagnostic reagent development only; not for direct clinical use.\u003cbr\u003e.   Use promptly after opening and store sealed to prevent evaporation and contamination.\u003cbr\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"10mg","offer_id":42989601849419,"sku":"UA086151-10mg","price":1250.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_3464fc8d-083f-4ed9-b377-fceba37c75a8.png?v=1785916837","url":"https:\/\/www.antbioinc.com\/products\/receptor-beads-ua086151","provider":"AntBio","version":"1.0","type":"link"}