{"product_id":"raw2647-ua090049","title":"Raw264.7 Osteoclast Differentiation Cytokine Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eMouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLyophilized powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReconstitution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\n\u003cspan style=\"color: rgb(51, 51, 51);font-size: 14px\"\u003eReconstitute at 0.1-1 mg\/ml according to the size in ultrapure water after rapid centrifugation.\u003c\/span\u003e\n\n\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan style=\"color: rgb(51, 51, 51);font-size: 14px\"\u003e·12 months from date of receipt, lyophilized powder stored at -20 to -80℃.\u003c\/span\u003e\u003cbr\u003e\u003cspan style=\"color: rgb(51, 51, 51);font-size: 14px\"\u003e·3 months, -20 to -80℃ under sterile conditions after reconstitution.\u003c\/span\u003e\u003cbr\u003e\u003cspan style=\"color: rgb(51, 51, 51);font-size: 14px\"\u003e·1 week, 2 to 8℃ under sterile conditions after reconstitution.\u003c\/span\u003e\u003cbr\u003e\u003cspan style=\"color: rgb(51, 51, 51);font-size: 14px\"\u003e·Please avoid repeated freeze-thaw cycles.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;width:392.5pt;\" border=\"0\" cellspacing=\"0\" cellpadding=\"0\" width=\"523\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height:16.5pt;\"\u003e\n\u003ctd style=\"border:solid black 1.0pt;height:16.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:95.5pt;\" width=\"127\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eproduct number\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid black 1.0pt;height:16.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:48.0pt;\" width=\"64\" valign=\"top\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eProtein\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid black 1.0pt;height:16.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:105.0pt;\" width=\"140\" valign=\"top\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eReference dosage\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid black 1.0pt;height:16.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:48.0pt;\" width=\"64\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eS Size\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid black 1.0pt;height:16.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:48.0pt;\" width=\"64\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eM Size\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid black 1.0pt;height:16.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:48.0pt;\" width=\"64\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eL Size\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:14.5pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid black 1.0pt;height:14.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:95.5pt;\" width=\"127\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003ca target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.ua-bio.com\/goodsList.html?keyword=UA040401\"\u003e\u003cspan style=\"color:#0057B5;\"\u003e\u003cspan style='font-family:\"Arial\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e\u003cstrong\u003eUA040401\u003c\/strong\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:14.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:48.0pt;\" width=\"64\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eRANKL\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:14.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:105.0pt;\" width=\"140\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e100ng\/ml\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:14.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:48.0pt;\" width=\"64\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e10ug\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:14.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:48.0pt;\" width=\"64\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e100ug\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:14.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:48.0pt;\" width=\"64\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e1mg\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:14.5pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid black 1.0pt;height:14.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:95.5pt;\" width=\"127\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003ca target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.ua-bio.com\/ua040128_m_csf_protein_mouse.html\"\u003e\u003cspan style=\"color:#0057B5;\"\u003e\u003cspan style='font-family:\"Arial\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e\u003cstrong\u003eUA040128\u003c\/strong\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:14.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:48.0pt;\" width=\"64\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eM-CSF\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:14.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:105.0pt;\" width=\"140\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e50ng\/ml\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:14.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:48.0pt;\" width=\"64\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e5ug\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:14.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:48.0pt;\" width=\"64\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e50ug\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:14.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:48.0pt;\" width=\"64\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e500ug\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:7.5pt;' lang=\"EN-US\"\u003eOsteoclast Precursor Cell Seeding\u003c\/span\u003e\u003cbr\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:7.5pt;' lang=\"EN-US\"\u003e1.1 Resuspend RAW 264.7 cells in good growth condition in α-MEM complete medium supplemented with 10% fetal bovine serum (FBS), and perform a cell count.\u003c\/span\u003e\u003cbr\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:7.5pt;' lang=\"EN-US\"\u003e1.2 Seed the cells at a density of 2.5 × 10³ cells\/mL into a 24-well cell culture plate pre-loaded with sterile glass coverslips. Add 1 mL of the cell suspension to each well.\u003c\/span\u003e\u003cbr\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:7.5pt;' lang=\"EN-US\"\u003e1.3 Incubate the plate in a 37°C, 5% CO₂ cell culture incubator for 18 hours to allow for complete cell adhesion.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:7.5pt;' lang=\"EN-US\"\u003eOsteoclast Induction \u003c\/span\u003e\u003cbr\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:7.5pt;' lang=\"EN-US\"\u003e2.1 After cell adhesion (approximately 18 hours post-seeding), aspirate the old medium from the wells.\u003c\/span\u003e\u003cbr\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:7.5pt;' lang=\"EN-US\"\u003e2.2 Replace with fresh osteoclast induction complete medium. The induction medium consists of α-MEM medium supplemented with 10% FBS, 50 ng\/mL recombinant mouse M-CSF protein, and 100 ng\/mL recombinant mouse RANK L protein.\u003c\/span\u003e\u003cbr\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:7.5pt;' lang=\"EN-US\"\u003e2.3 Replace the induction medium every 3 days. During each change, gently aspirate the old medium and add an equal volume of fresh induction medium containing the same concentrations of M-CSF and RANK L proteins.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:7.5pt;' lang=\"EN-US\"\u003eCell Morphological Observation and Identification\u003c\/span\u003e\u003cbr\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:7.5pt;' lang=\"EN-US\"\u003e3.1 Morphological Observation: Observe changes in cell morphology regularly under an inverted microscope starting from the initiation of induction. Typically, typical multinucleated osteoclast-like structures can be observed after 4 days of RANK L induction.\u003c\/span\u003e\u003cbr\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:7.5pt;' lang=\"EN-US\"\u003e3.2 TRAP Staining Verification: After 5 days of RANK L induction, perform tartrate-resistant acid phosphatase (TRAP) staining to specifically identify osteoclasts. Strictly follow the instructions of the TRAP staining kit for the staining procedure. Upon completion of staining, observe under a light microscope. The cytoplasm of mature osteoclasts appears red or purple-red.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"S","offer_id":42302156996683,"sku":null,"price":225.0,"currency_code":"USD","in_stock":true},{"title":"M","offer_id":42302157029451,"sku":null,"price":1035.0,"currency_code":"USD","in_stock":true},{"title":"L","offer_id":42302157062219,"sku":null,"price":4670.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/98ea9eaf-8e58-453c-ae67-a68cf996774d.png?v=1776073521","url":"https:\/\/www.antbioinc.com\/products\/raw2647-ua090049","provider":"AntBio","version":"1.0","type":"link"}