{"product_id":"rat-ifn","title":"Rat IFN-γ ELISA KIT","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Principle: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe double antibody sandwich ELISA method was used in this experiment. Anti rat \u003cspan style=\"font-size: 10pt;\"\u003eIFN-γ\u003c\/span\u003eThe monoclonal antibody was coated on the microplate, and \u003cspan style=\"font-size: 10pt;\"\u003eIFN-γ\u003c\/span\u003e in samples and standards; It will bind to the antibody fixed on the plate, and the free components will be washed away; Biotin labeled anti rat \u003cspan style=\"font-size: 10pt;\"\u003eIFN-γ\u003c\/span\u003e was added; Polyclonal antibody, and \u003cspan style=\"font-size: 10pt;\"\u003eIFN-γ\u003c\/span\u003e bound to the microplate; Binding, free components are washed away; The streptavidin labeled horseradish peroxidase, which specifically recognizes biotin, is added to form a complex. After washing away the free components, the substrate solution (chromogenic agent) is added, and the color of the solution gradually turns blue. After adding the stop solution, the solution turns yellow and stops changing. The absorbance was measured with a microplate reader.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Type: \u003c\/strong\u003eDouble antibody sandwich method\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm: \u003c\/strong\u003ePre coated 96 well plate\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Sample Type: \u003c\/strong\u003ecell supernatant, serum, Plasma\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLoading Amount: \u003c\/strong\u003e100ul\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003ePrecoated 96 well plates, standards, anti rat IFN - γ One copy of detection antibody, dilution buffer, chromogenic solution (a, b), washing solution, termination solution, sa-hrp, plate sealing membrane and instructions\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity: \u003c\/strong\u003e15.6 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDetection Range: \u003c\/strong\u003e39.1 - 2500 pg\/ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery Range: \u003c\/strong\u003e89.0-118.9%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Method: \u003c\/strong\u003e2-8 ℃  span\u0026gt;\u003c\/p\u003e\n\u003cstrong\u003eStandard Curve \u003c\/strong\u003e\u003cp\u003e\u003cimg src=\"https:\/\/univ.oss-cn-shanghai.aliyuncs.com\/absin-china\/20200827\/fc85552aa7ec48eca11523a6959f1648.jpg\" alt=\"\" width=\"300\" height=\"233\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground: \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIFN-γ (interferon -γ)It is a typical proinflammatory cytokine produced by a variety of immune cells under inflammatory conditions, especially by T cells and NK cells. It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen-presenting molecules, and immunoglobulin class switching in B cells. It also has antiviral, antiproliferative and apoptotic effects. In addition, IFN-γ Acts as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. Ifn-γ Dimers pass through two ifn-γ R1 and two ifn-γ The receptor complex of the R2 subunit signals.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":43115011309643,"sku":"abs530004-96T","price":450.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.antbioinc.com\/products\/rat-ifn","provider":"AntBio","version":"1.0","type":"link"}