{"product_id":"parp1-knockout-hela-cell-lysate-s0y0027","title":"PARP1-Knockout HeLa Cell Lysate","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMutations\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eMutation in exon4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eWB\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e2 mg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eSupplied in SDS Sample Buffer: 50 mM Tris-HCl (pH 6.8 at 25°C), 2% w\/v SDS, 10% glycerol, 1% v\/v β-Mercaptoethanol, 0.01% w\/v bromophenol blue or phenol red.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-20 °C for 1 months under sterile conditions; \u003cbr\u003e-80 °C for 24 months under sterile conditions.\u003cbr\u003ePlease avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan\u003ePARP1 (poly [ADP-ribose] polymerase 1) is a highly abundant nuclear enzyme that plays a central role in the detection and repair of DNA damage. Upon sensing DNA single-strand breaks and other genomic lesions, PARP1 uses NAD+ as a substrate to synthesize poly (ADP-ribose) chains on itself and multiple target proteins, a process known as PARylation. This modification recruits and regulates DNA repair factors, promotes chromatin remodeling, and facilitates several DNA damage response pathways, particularly base excision repair and single-strand break repair. PARP1 is also involved in the regulation of transcription, replication stress responses, chromatin organization, and cell-death signaling. During apoptosis, PARP1 is cleaved by activated caspase-3 and caspase-7 into characteristic approximately 89 kDa and 24 kDa fragments; therefore, detection of cleaved PARP1 is widely used as a biochemical marker of caspase-dependent apoptosis. PARP1 antibodies are commonly used in Western blotting, immunohistochemistry, immunofluorescence, and flow cytometry to assess PARP1 expression, DNA damage responses, and apoptotic signaling. PARP1 knockout (KO) may impair efficient repair of DNA single-strand breaks, alter cellular responses to replication stress and genotoxic agents, and increase dependence on compensatory DNA repair pathways; however, the resulting phenotype can vary substantially with cell type, genetic background, and the nature of the DNA-damaging stimulus.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1kit","offer_id":43160888377419,"sku":"S0Y0027-1kit","price":665.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/1d1c5e098cf246ca821d8d72fb797ed2_51bbc74b-52d0-47b2-81c8-719608b0311a.jpg?v=1788768058","url":"https:\/\/www.antbioinc.com\/products\/parp1-knockout-hela-cell-lysate-s0y0027","provider":"AntBio","version":"1.0","type":"link"}