{"product_id":"parg-his-tag-protein-mouse-ua085045","title":"PARG His tag Protein, Mouse","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003ePoly(ADP-ribose) glycohydrolase, PARG\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAccession\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eO88622-1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAmino Acid Sequence\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eMet1-Thr969 with His Tag at the C-Terminus\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eE.coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e20-25kDa (Reducing)\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e＞90% by SDS-PAGE,\u0026gt; 95% by HPLC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHis Tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e50mM Tris, 200mM NaCl, 20% Glycerol, 1mM DTT, pH7.5\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eSun Y, Chen J, Huang SN, Su YP, Wang W, Agama K, Saha S, Jenkins LM, Pascal JM, Pommier Y. PARylation prevents the proteasomal degradation of topoisomerase I DNA-protein crosslinks and induces their deubiquitylation. Nat Commun. 2021 Aug 18;12(1):5010. \u003cbr\u003eFischbach A, Bangert K, Bürkle A, Mangerich A. Noncovalent PAR Binding Guides Proteins to PARP1-Mediated PARylation. ACS Chem Biol. 2026 Mar 13.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan\u003ePARG (Poly(ADP-ribose) Glycohydrolase) serves as the key negative regulator of PAR metabolism, belonging to the glycoside hydrolase family that modulates protein PARylation levels through hydrolysis of glycosidic bonds within PAR chains. Structurally, PARG comprises an N-terminal regulatory domain and a C-terminal catalytic domain; the catalytic domain forms an α\/β-barrel fold with an active site constituted by a catalytic triad of Glu755\/Glu756\/Asp737, executing exoglycosidase activity by binding to terminal ADP-ribose units of PAR chains, with His-tags commonly employed for recombinant protein purification. Functionally, PARG maintains dynamic equilibrium with PARPs (particularly PARP1\/2) to rapidly degrade PAR chains and preserve cellular PARylation homeostasis, thereby regulating DNA damage repair, replication fork restart, transcriptional control, and cell death; PARG deficiency leads to PAR chain accumulation, triggering replication stress and genomic instability. Clinically, PARG inhibitors (such as COH34 and PDD00017273) exhibit synthetic lethality with PARP inhibitors, demonstrating therapeutic potential for BRCA-mutant tumors and PARP inhibitor-resistant patients, currently positioned at preclinical\/early clinical development stages as an emerging target for synthetic lethal strategies.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"10μg","offer_id":43093483814987,"sku":"UA085045-10μg","price":730.0,"currency_code":"USD","in_stock":true},{"title":"50μg","offer_id":43093483847755,"sku":"UA085045-50μg","price":1820.0,"currency_code":"USD","in_stock":true},{"title":"100μg","offer_id":43093483880523,"sku":"UA085045-100μg","price":2910.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/c0f3080066c8406c97acf33b9ea46118.png?v=1788192226","url":"https:\/\/www.antbioinc.com\/products\/parg-his-tag-protein-mouse-ua085045","provider":"AntBio","version":"1.0","type":"link"}