{"product_id":"papp-a-his-tag-protein-human-ua016163","title":"PAPP-A His Tag Protein, Human","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003ePappalysin-1, PAPPA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAccession\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eQ13219\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAmino Acid Sequence\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eGlu82-Gly1627 with His Tag at the C-Terminus\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHEK293\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e50-55kDa\u0026amp;170-180kDa\u0026amp;＞180kDa (Reducing)\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e＞95% by SDS-PAGE \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHis Tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLyophilized powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003ePBS, pH7.4, 5% trehalose\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReconstitution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eReconstitute at 0.1-1 mg\/ml according to the size in ultrapure water after rapid centrifugation.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e· 3 months, -20 to -80℃ under sterile conditions after reconstitution.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e· 1 week, 2 to 8℃ under sterile conditions after reconstitution.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e· Please avoid repeated freeze-thaw cycles.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e1.Lawrence JB, Oxvig C, Overgaard MT, Sottrup-Jensen L, Gleich GJ, Hays LG, Yates JR 3rd, Conover CA. The insulin-like growth factor (IGF)-dependent IGF binding protein-4 protease secreted by human fibroblasts is pregnancy-associated plasma protein-A. Proc Natl Acad Sci U S A. 1999 Mar 16;96(6):3149-53. \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan\u003ePAPP-A (Pregnancy-Associated Plasma Protein-A) is a zinc metalloproteinase comprising multiple functional modules including LNR1, LNR2, M1-M5 metalloprotease domains, and CPD (CUB and protease-like domain); its activity is covalently regulated by endogenous inhibitors proMBP and STC2 through steric hindrance mechanisms, and cryo-EM structural studies published in Cell Discovery and Nature Communications in 2024 first resolved the three-dimensional structures of these complexes, laying the foundation for targeted drug design; functionally, PAPP-A specifically cleaves insulin-like growth factor binding proteins (IGFBP-2, -4, -5) to release active IGF-I\/II, serving as a key regulator of the IGF signaling pathway involved in fetal growth and development, bone formation, tissue repair, and metabolic regulation; clinically, PAPP-A is a core serum biomarker for first-trimester Down syndrome screening (low levels indicating fetal aneuploidy risk), with low PAPP-A also associated with fetal growth restriction, preeclampsia, and preterm delivery, while elevated levels are important biomarkers for atherosclerotic plaque instability and acute coronary syndrome, and its overexpression in ovarian cancer, breast cancer, Ewing sarcoma, and other malignancies promotes tumor progression, making it a potential target for cardiovascular risk assessment and antitumor therapy.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cstrong\u003eAssay protocol\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ePrinciple:\u003c\/strong\u003e\u003cspan\u003e As an active protease, recombinant human PAPP-A cleaves IGFBP-5 under the conditions described in Activity Assay Protocol.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eMaterials\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"margin-right:8px;\"\u003e1.\u003c\/span\u003e\u003cspan\u003eAssay Buffer: 50 mM Tris, 10 mM CaCl\u003csub\u003e2\u003c\/sub\u003e, 150 mM NaCl (TCN)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"margin-right:8px;\"\u003e2.\u003c\/span\u003e\u003cspan\u003ePAPP-A His Tag Protein, Human\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"margin-right:8px;\"\u003e3.\u003c\/span\u003e\u003cspan\u003eHuman IGFBP5 Protein, His Tag (S0A0185)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"margin-right:8px;\"\u003e4.\u003c\/span\u003e\u003cspan\u003e4-20% SDS-PAGE gel\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"margin-right:8px;\"\u003e5.\u003c\/span\u003e\u003cspan\u003e5×reducing SDS-PAGE gel buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"margin-right:8px;\"\u003e6.\u003c\/span\u003e\u003cspan\u003eSDS-PAGE\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProduce\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1. Dilute PAPP-A to 10 µg\/mL, in Assay Buffer.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2. Dilute rhIGFBP-5 to 200 µg\/mL in Assay Buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3. Combine equal volumes of 10 µg\/mL PAPP-A and 200 µg\/mL rhIGFBP-5. Include two blank controls containing Assay Buffer in place of PAPP-A.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e4. Incubate reaction vials and one control at 37 °C for 30 minutes. Keep the other control at -20 °C during the incubation period.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e5. Combine 20 µL from each reaction vial with 5µL 5×reducing SDS-PAGE gel buffer.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e6. Analyze the cleavage by SDS PAGE (load 20 µL\/lane) followed by protein staining.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"25μg","offer_id":43096734531659,"sku":"UA016163-25μg","price":320.0,"currency_code":"USD","in_stock":true},{"title":"100μg","offer_id":43096734564427,"sku":"UA016163-100μg","price":630.0,"currency_code":"USD","in_stock":true},{"title":"500μg","offer_id":43096734597195,"sku":"UA016163-500μg","price":1615.0,"currency_code":"USD","in_stock":true},{"title":"1mg","offer_id":43096734629963,"sku":"UA016163-1mg","price":2420.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/d596d17cf3604f4f8fe79abd71ac0cef.png?v=1788242525","url":"https:\/\/www.antbioinc.com\/products\/papp-a-his-tag-protein-human-ua016163","provider":"AntBio","version":"1.0","type":"link"}