{"product_id":"nek7-his-tag-protein-human-ua085017","title":"NEK7 His Tag Protein, Human","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eNimA-related protein kinase 7, Never in mitosis A-related kinase 7\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAccession\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eQ8TDX7\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAmino Acid Sequence\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eMet1-Ser302 with His Tag at the N-Terminus\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eE.coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e35-40kDa (Reducing)\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e＞85% by SDS-PAGE \u0026amp; \u0026gt; 90% by HPLC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHis Tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e50mM Tris, 150mM NaCl, pH7.5, 1mM DTT, 10%Glycerol\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e1. Shi, H., et al. (2016). NLRP3 activation and mitosis are mutually exclusive events coordinated by NEK7. Nature, 540(7631), 440-445.\u003cbr\u003e2. He, Y., Zeng, M.Y., Yang, D., Motro, B., \u0026amp; Núñez, G. (2016). NEK7 is an essential mediator of NLRP3 activation downstream of potassium efflux. Nature, 540(7631), 123-127.\u003cbr\u003e3. Kim, S., Lee, K., \u0026amp; Rhee, K. (2007). NEK7 is a centrosomal kinase critical for microtubule nucleation. Biochemical and Biophysical Research Communications, 360(1), 56-62.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cspan\u003eNEK7 (NIMA-related kinase 7) is a serine\/threonine kinase belonging to the NEK family. It plays a crucial and specific role in regulating cell cycle progression, particularly in mitotic entry and spindle assembly. Structurally distinct from other NEK kinases, NEK7 contains an N-terminal catalytic kinase domain and a C-terminal regulatory domain. Its activity is tightly controlled by cell cycle-dependent phosphorylation and its interaction with NEK9. The NEK9\/NEK6\/NEK7 cascade is a key pathway for mitotic centrosome separation and spindle formation.\u003c\/span\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"line-height:150%;text-align:center;\"\u003e\u003cspan style=\"line-height:150%;\"\u003e\u003cstrong\u003eExperimental Methods\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"line-height:150%;\"\u003e\u003cspan style=\"line-height:150%;\"\u003e\u003cstrong\u003eExperimental Principle\u003c\/strong\u003e: The ADP-Glo™ Kinase Assay Kit is used to measure NEK7 activity by quantifying the ADP produced in the reaction. The steps are as follows: First, the ADP-Glo™ Reagent is added to terminate the kinase reaction and deplete residual ATP. Then, the Kinase Detection Reagent is added to convert ADP into ATP, which is detected using a luciferase\/luciferin reaction system.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"line-height:150%;\"\u003e\u003cspan style=\"line-height:150%;\"\u003e\u003cstrong\u003eExperimental Materials\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003col style=\"padding-left:24px;\"\u003e\n\u003cli\u003e\u003cp style=\"line-height:150%;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;line-height:150%;' lang=\"EN-US\"\u003eKinase assay buffer (5X): 200 mM Tris-HCl, pH 7.4, 100 mM MgCl\u003csub\u003e2\u003c\/sub\u003e, 0.5 mg\/mL BSA, 250 μM DTT\u003c\/span\u003e\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp style=\"line-height:150%;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;line-height:150%;' lang=\"EN-US\"\u003eKinase assay buffer (1X): 40 mM Tris-HCl, pH 7.4, 20 mM MgCl\u003csub\u003e2\u003c\/sub\u003e, 0.1 mg\/mL BSA, 50 μM DTT\u003c\/span\u003e\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp style=\"line-height:150%;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;line-height:150%;' lang=\"EN-US\"\u003eNEK7 His Tag Protein, Human\u003c\/span\u003e\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp style=\"line-height:150%;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;line-height:150%;' lang=\"EN-US\"\u003eADP-Glo Kinase Assay (Promega, Catalog # V6930)\u003c\/span\u003e\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp style=\"line-height:150%;\"\u003e\u003cspan style=\"line-height:150%;\"\u003eSubstrate: Casein substrate (Sinobiological, Catalog # C03-54N)\u003c\/span\u003e\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp style=\"line-height:150%;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;line-height:150%;' lang=\"EN-US\"\u003eSolid white multi-well plate (384-well plate) (Corning, Catalog #3572)\u003c\/span\u003e\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp style=\"line-height:150%;\"\u003e\u003cspan style=\"line-height:150%;\"\u003ePlate reader (\u003c\/span\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;line-height:150%;' lang=\"EN-US\"\u003ePerkinElmer\u003c\/span\u003e\u003cspan style=\"line-height:150%;\"\u003e)\u003c\/span\u003e\u003c\/p\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp style=\"line-height:150%;\"\u003e\u003cspan style=\"line-height:150%;\"\u003e\u003cstrong\u003eExperimental Procedure\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003col style=\"padding-left:24px;\"\u003e\u003cli\u003e\u003cp style=\"line-height:150%;\"\u003e\u003cspan style=\"line-height:150%;\"\u003ePrepare the substrate\/ATP mixture as follows (using 25 μM as an example):\u003c\/span\u003e\u003c\/p\u003e\u003c\/li\u003e\u003c\/ol\u003e\n\u003cdiv align=\"center\"\u003e\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:none;\" border=\"1\" cellspacing=\"0\" cellpadding=\"0\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:207.4pt;\" width=\"277\" valign=\"top\"\u003e\u003cp style=\"line-height:150%;text-align:center;text-indent:0cm;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;line-height:150%;' lang=\"EN-US\"\u003e\u003cstrong\u003eSample Name\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:207.4pt;\" width=\"277\" valign=\"top\"\u003e\u003cp style=\"line-height:150%;text-align:center;text-indent:0cm;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;line-height:150%;' lang=\"EN-US\"\u003e\u003cstrong\u003eAmount (μL)\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:207.4pt;\" width=\"277\" valign=\"top\"\u003e\u003cp style=\"line-height:150%;text-align:center;text-indent:0cm;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;line-height:150%;' lang=\"EN-US\"\u003e10 mM ATP Solution\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:207.4pt;\" width=\"277\" valign=\"top\"\u003e\u003cp style=\"line-height:150%;text-align:center;text-indent:0cm;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;line-height:150%;' lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:207.4pt;\" width=\"277\" valign=\"top\"\u003e\u003cp style=\"line-height:150%;text-align:center;text-indent:0cm;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;line-height:150%;' lang=\"EN-US\"\u003eKinase Assay Buffer III (5x)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:207.4pt;\" width=\"277\" valign=\"top\"\u003e\u003cp style=\"line-height:150%;text-align:center;text-indent:0cm;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;line-height:150%;' lang=\"EN-US\"\u003e79\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:207.4pt;\" width=\"277\" valign=\"top\"\u003e\u003cp style=\"line-height:150%;text-align:center;text-indent:0cm;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;line-height:150%;' lang=\"EN-US\"\u003eSubstrate at 1 mg\/mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:207.4pt;\" width=\"277\" valign=\"top\"\u003e\u003cp style=\"line-height:150%;text-align:center;text-indent:0cm;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;line-height:150%;' lang=\"EN-US\"\u003e80\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003c\/div\u003e\n\u003cp style=\"line-height:150%;\"\u003e \u003c\/p\u003e\n\u003col style=\"padding-left:24px;\" start=\"2\"\u003e\n\u003cli\u003e\u003cp style=\"line-height:150%;\"\u003e\u003cspan style=\"line-height:150%;\"\u003eDilute NEK7 to 15 µg\/mL, 7.5 µg\/mL, and 3.75 µg\/mL using kinase assay buffer (1x), and add 3 µL to each well of a 384-well plate.\u003c\/span\u003e\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp style=\"line-height:150%;\"\u003e\u003cspan style=\"line-height:150%;\"\u003eInitiate the reaction by adding 2 µL of the assay system prepared in step 1 to each well. Include a blank control with only 3 µL of kinase assay buffer (1x). The total reaction volume is 5 µL. Incubate the reaction at room temperature (22–25°C) for 40 minutes.\u003c\/span\u003e\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp style=\"line-height:150%;\"\u003e\u003cspan style=\"line-height:150%;\"\u003eAdd 5 µL of ADP-Glo Reagent to the completed reaction system, mix briefly, and incubate at room temperature (22–25°C) for 40 minutes.\u003c\/span\u003e\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp style=\"line-height:150%;\"\u003e\u003cspan style=\"line-height:150%;\"\u003eAdd 10 µL of Detection Reagent and incubate the plate at room temperature (22–25°C) for 30 minutes.\u003c\/span\u003e\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp style=\"line-height:150%;\"\u003e\u003cspan style=\"line-height:150%;\"\u003eRead the chemiluminescence signal in endpoint mode.\u003c\/span\u003e\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp style=\"line-height:150%;\"\u003e\u003cspan style=\"line-height:150%;\"\u003eCalculate the specific activity.\u003c\/span\u003e\u003c\/p\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp style=\"line-height:150%;\"\u003e\u003cspan style=\"line-height:150%;\"\u003e\u003cstrong\u003eStandard Curve\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003col style=\"padding-left:24px;\"\u003e\n\u003cli\u003e\u003cp style=\"line-height:150%;\"\u003e\u003cspan style=\"line-height:150%;\"\u003eDilute ATP and ADP to 25 μM in kinase assay buffer (1×).\u003c\/span\u003e\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp style=\"line-height:150%;\"\u003e\u003cspan style=\"line-height:150%;\"\u003eMix 25 μM ATP and 25 μM ADP as shown in the table below to prepare ATP+ADP mixtures, and add 5 μL to each well of a 384-well plate.\u003c\/span\u003e\u003c\/p\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:none;\" border=\"1\" cellspacing=\"0\" cellpadding=\"0\" width=\"543\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height:10.8pt;\"\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;height:10.8pt;padding:0cm 5.4pt 0cm 5.4pt;width:49.4pt;\" width=\"66\" valign=\"top\"\u003e\u003cp style=\"line-height:150%;text-align:center;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:5.0pt;line-height:150%;' lang=\"EN-US\"\u003e\u003cstrong\u003eWell Number\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid windowtext 1.0pt;height:10.8pt;padding:0cm 5.4pt 0cm 5.4pt;width:1.0cm;\" width=\"38\" valign=\"top\"\u003e\u003cp style=\"line-height:150%;text-align:center;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:5.0pt;line-height:150%;' lang=\"EN-US\"\u003e\u003cstrong\u003e1\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid windowtext 1.0pt;height:10.8pt;padding:0cm 5.4pt 0cm 5.4pt;width:1.0cm;\" width=\"38\" valign=\"top\"\u003e\u003cp style=\"line-height:150%;text-align:center;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:5.0pt;line-height:150%;' lang=\"EN-US\"\u003e\u003cstrong\u003e2\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid windowtext 1.0pt;height:10.8pt;padding:0cm 5.4pt 0cm 5.4pt;width:25.45pt;\" width=\"34\" valign=\"top\"\u003e\u003cp style=\"line-height:150%;text-align:center;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:5.0pt;line-height:150%;' lang=\"EN-US\"\u003e\u003cstrong\u003e3\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid windowtext 1.0pt;height:10.8pt;padding:0cm 5.4pt 0cm 5.4pt;width:30.65pt;\" width=\"41\" valign=\"top\"\u003e\u003cp style=\"line-height:150%;text-align:center;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:5.0pt;line-height:150%;' lang=\"EN-US\"\u003e\u003cstrong\u003e4\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid windowtext 1.0pt;height:10.8pt;padding:0cm 5.4pt 0cm 5.4pt;width:30.65pt;\" width=\"41\" valign=\"top\"\u003e\u003cp style=\"line-height:150%;text-align:center;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:5.0pt;line-height:150%;' lang=\"EN-US\"\u003e\u003cstrong\u003e5\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid windowtext 1.0pt;height:10.8pt;padding:0cm 5.4pt 0cm 5.4pt;width:30.65pt;\" width=\"41\" valign=\"top\"\u003e\u003cp style=\"line-height:150%;text-align:center;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:5.0pt;line-height:150%;' lang=\"EN-US\"\u003e\u003cstrong\u003e6\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid windowtext 1.0pt;height:10.8pt;padding:0cm 5.4pt 0cm 5.4pt;width:30.65pt;\" width=\"41\" valign=\"top\"\u003e\u003cp style=\"line-height:150%;text-align:center;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:5.0pt;line-height:150%;' lang=\"EN-US\"\u003e\u003cstrong\u003e7\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid windowtext 1.0pt;height:10.8pt;padding:0cm 5.4pt 0cm 5.4pt;width:30.65pt;\" width=\"41\" valign=\"top\"\u003e\u003cp style=\"line-height:150%;text-align:center;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:5.0pt;line-height:150%;' lang=\"EN-US\"\u003e\u003cstrong\u003e8\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid windowtext 1.0pt;height:10.8pt;padding:0cm 5.4pt 0cm 5.4pt;width:30.65pt;\" width=\"41\" valign=\"top\"\u003e\u003cp style=\"line-height:150%;text-align:center;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:5.0pt;line-height:150%;' lang=\"EN-US\"\u003e\u003cstrong\u003e9\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid windowtext 1.0pt;height:10.8pt;padding:0cm 5.4pt 0cm 5.4pt;width:30.65pt;\" width=\"41\" valign=\"top\"\u003e\u003cp style=\"line-height:150%;text-align:center;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:5.0pt;line-height:150%;' lang=\"EN-US\"\u003e\u003cstrong\u003e10\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid windowtext 1.0pt;height:10.8pt;padding:0cm 5.4pt 0cm 5.4pt;width:30.7pt;\" width=\"41\" valign=\"top\"\u003e\u003cp style=\"line-height:150%;text-align:center;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:5.0pt;line-height:150%;' lang=\"EN-US\"\u003e\u003cstrong\u003e11\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid windowtext 1.0pt;height:10.8pt;padding:0cm 5.4pt 0cm 5.4pt;width:30.7pt;\" width=\"41\" valign=\"top\"\u003e\u003cp style=\"line-height:150%;text-align:center;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:5.0pt;line-height:150%;' lang=\"EN-US\"\u003e\u003cstrong\u003e12\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:13.55pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:13.55pt;padding:0cm 5.4pt 0cm 5.4pt;width:49.4pt;\" width=\"66\" valign=\"top\"\u003e\u003cp style=\"line-height:150%;text-align:center;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:5.0pt;line-height:150%;' lang=\"EN-US\"\u003e25μM ADP (μL)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:13.55pt;padding:0cm 5.4pt 0cm 5.4pt;width:1.0cm;\" width=\"38\" valign=\"top\"\u003e\u003cp style=\"line-height:150%;text-align:center;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:5.0pt;line-height:150%;' lang=\"EN-US\"\u003e100\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:13.55pt;padding:0cm 5.4pt 0cm 5.4pt;width:1.0cm;\" width=\"38\" valign=\"top\"\u003e\u003cp style=\"line-height:150%;text-align:center;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:5.0pt;line-height:150%;' lang=\"EN-US\"\u003e80\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:13.55pt;padding:0cm 5.4pt 0cm 5.4pt;width:25.45pt;\" width=\"34\" valign=\"top\"\u003e\u003cp style=\"line-height:150%;text-align:center;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:5.0pt;line-height:150%;' lang=\"EN-US\"\u003e60\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:13.55pt;padding:0cm 5.4pt 0cm 5.4pt;width:30.65pt;\" width=\"41\" valign=\"top\"\u003e\u003cp style=\"line-height:150%;text-align:center;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:5.0pt;line-height:150%;' lang=\"EN-US\"\u003e40\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:13.55pt;padding:0cm 5.4pt 0cm 5.4pt;width:30.65pt;\" width=\"41\" valign=\"top\"\u003e\u003cp style=\"line-height:150%;text-align:center;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:5.0pt;line-height:150%;' lang=\"EN-US\"\u003e20\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:13.55pt;padding:0cm 5.4pt 0cm 5.4pt;width:30.65pt;\" width=\"41\" valign=\"top\"\u003e\u003cp style=\"line-height:150%;text-align:center;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:5.0pt;line-height:150%;' lang=\"EN-US\"\u003e10\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:13.55pt;padding:0cm 5.4pt 0cm 5.4pt;width:30.65pt;\" width=\"41\" valign=\"top\"\u003e\u003cp style=\"line-height:150%;text-align:center;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:5.0pt;line-height:150%;' lang=\"EN-US\"\u003e5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:13.55pt;padding:0cm 5.4pt 0cm 5.4pt;width:30.65pt;\" width=\"41\" valign=\"top\"\u003e\u003cp style=\"line-height:150%;text-align:center;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:5.0pt;line-height:150%;' lang=\"EN-US\"\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:13.55pt;padding:0cm 5.4pt 0cm 5.4pt;width:30.65pt;\" width=\"41\" valign=\"top\"\u003e\u003cp style=\"line-height:150%;text-align:center;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:5.0pt;line-height:150%;' lang=\"EN-US\"\u003e3\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:13.55pt;padding:0cm 5.4pt 0cm 5.4pt;width:30.65pt;\" width=\"41\" valign=\"top\"\u003e\u003cp style=\"line-height:150%;text-align:center;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:5.0pt;line-height:150%;' lang=\"EN-US\"\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:13.55pt;padding:0cm 5.4pt 0cm 5.4pt;width:30.7pt;\" width=\"41\" valign=\"top\"\u003e\u003cp style=\"line-height:150%;text-align:center;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:5.0pt;line-height:150%;' lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:13.55pt;padding:0cm 5.4pt 0cm 5.4pt;width:30.7pt;\" width=\"41\" valign=\"top\"\u003e\u003cp style=\"line-height:150%;text-align:center;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:5.0pt;line-height:150%;' lang=\"EN-US\"\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:7.75pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:7.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:49.4pt;\" width=\"66\" valign=\"top\"\u003e\u003cp style=\"line-height:150%;text-align:center;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:5.0pt;line-height:150%;' lang=\"EN-US\"\u003e25μM ATP (μL)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:7.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:1.0cm;\" width=\"38\" valign=\"top\"\u003e\u003cp style=\"line-height:150%;text-align:center;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:5.0pt;line-height:150%;' lang=\"EN-US\"\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:7.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:1.0cm;\" width=\"38\" valign=\"top\"\u003e\u003cp style=\"line-height:150%;text-align:center;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:5.0pt;line-height:150%;' lang=\"EN-US\"\u003e20\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:7.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:25.45pt;\" width=\"34\" valign=\"top\"\u003e\u003cp style=\"line-height:150%;text-align:center;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:5.0pt;line-height:150%;' lang=\"EN-US\"\u003e40\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:7.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:30.65pt;\" width=\"41\" valign=\"top\"\u003e\u003cp style=\"line-height:150%;text-align:center;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:5.0pt;line-height:150%;' lang=\"EN-US\"\u003e60\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:7.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:30.65pt;\" width=\"41\" valign=\"top\"\u003e\u003cp style=\"line-height:150%;text-align:center;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:5.0pt;line-height:150%;' lang=\"EN-US\"\u003e80\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:7.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:30.65pt;\" width=\"41\" valign=\"top\"\u003e\u003cp style=\"line-height:150%;text-align:center;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:5.0pt;line-height:150%;' lang=\"EN-US\"\u003e90\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:7.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:30.65pt;\" width=\"41\" valign=\"top\"\u003e\u003cp style=\"line-height:150%;text-align:center;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:5.0pt;line-height:150%;' lang=\"EN-US\"\u003e95\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:7.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:30.65pt;\" width=\"41\" valign=\"top\"\u003e\u003cp style=\"line-height:150%;text-align:center;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:5.0pt;line-height:150%;' lang=\"EN-US\"\u003e96\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:7.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:30.65pt;\" width=\"41\" valign=\"top\"\u003e\u003cp style=\"line-height:150%;text-align:center;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:5.0pt;line-height:150%;' lang=\"EN-US\"\u003e97\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:7.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:30.65pt;\" width=\"41\" valign=\"top\"\u003e\u003cp style=\"line-height:150%;text-align:center;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:5.0pt;line-height:150%;' lang=\"EN-US\"\u003e98\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:7.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:30.7pt;\" width=\"41\" valign=\"top\"\u003e\u003cp style=\"line-height:150%;text-align:center;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:5.0pt;line-height:150%;' lang=\"EN-US\"\u003e99\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:7.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:30.7pt;\" width=\"41\" valign=\"top\"\u003e\u003cp style=\"line-height:150%;text-align:center;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:5.0pt;line-height:150%;' lang=\"EN-US\"\u003e100\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp style=\"line-height:150%;\"\u003e \u003c\/p\u003e\n\u003col style=\"padding-left:24px;\" start=\"3\"\u003e\n\u003cli\u003e\u003cspan\u003eAdd 5 μL of ADP-Glo Reagent to the completed reaction wells, mix briefly, and incubate at room temperature (22-25°C) for 40 minutes.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eAdd 10 μL of Detection Reagent and incubate at room temperature (22-25°C) for 30 minutes.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eRead the chemiluminescence signal in endpoint mode.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eMeasure the light signal and establish the conversion curve.\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;\" border=\"0\" cellspacing=\"0\" cellpadding=\"0\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding:7.5pt 7.5pt 7.5pt 7.5pt;width:159.25pt;\" rowspan=\"2\" width=\"212\"\u003e\u003cp style=\"line-height:150%;margin-bottom:7.5pt;text-align:center;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:10.0pt;line-height:150%;' lang=\"EN-US\"\u003eSpecific Activity (pmol\/min\/μg) =\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border:none;padding:7.5pt 7.5pt 1.5pt 7.5pt;width:211.75pt;\" width=\"282\"\u003e\u003cp style=\"line-height:150%;text-align:center;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:10.0pt;line-height:150%;' lang=\"EN-US\"\u003eATP (pmol)-Blank\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border:none;padding:7.5pt 7.5pt 7.5pt 7.5pt;width:211.75pt;\" width=\"282\"\u003e\u003cp style=\"line-height:150%;text-align:center;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:10.0pt;line-height:150%;' lang=\"EN-US\"\u003eIncubation time(min) ×amount of enzyme (μg)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"10μg","offer_id":42332762243147,"sku":null,"price":515.0,"currency_code":"USD","in_stock":true},{"title":"50μg","offer_id":42332762275915,"sku":null,"price":1665.0,"currency_code":"USD","in_stock":true},{"title":"100μg","offer_id":42332762308683,"sku":null,"price":2830.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/8c851b66-deab-4cc7-9d4a-7c0355b3cca7.png?v=1780633691","url":"https:\/\/www.antbioinc.com\/products\/nek7-his-tag-protein-human-ua085017","provider":"AntBio","version":"1.0","type":"link"}