{"product_id":"mouse-tumor-tissue-digestion-solution-abs9644","title":"Mouse Intestinal Tumor Tissue Digestion Solution","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Before digesting the tissue sample, use ophthalmic scissors or a scalpel to cut the tissue into a volume of approximately 1-3 mm.\u003csup\u003e3\u003c\/sup\u003eTissue blocks.\u003cbr\u003e2. Add an appropriate volume of tissue digestive fluid (the digestive fluid volume should be 5-10 times the original tissue volume) according to the size of the original tissue block; Tissue digestion was performed in a 4℃ constant temperature incubator or constant temperature shaker, with an overall digestion time of 15-20 min.\u003cbr\u003e\u003cstrong\u003eNote:\u003c\/strong\u003eThe digestion process must be carefully monitored because excessive digestion may affect cell viability and significantly reduce the organoid success rate. During the digestion process, the digestion suspension can be microscopically examined. Digestion can be considered complete when a large number of individual cells or cell clusters smaller than 70 μm are observed under the microscope.\u003cbr\u003e3. Add 3 times organoid culture buffer to the tissue suspension after confirming the completion of digestion to terminate digestion.\u003cbr\u003e4. The digestion suspension obtained in the above step can be directly used for cell separation operations such as centrifugation or screen filtration. Before using the separated cells, the sample must be centrifuged and washed more than twice using organoid buffer before use (recommended centrifugation speed 200-300 g centrifugation for 3 min).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eMouse small intestine-specific primary tissue digestion solution can quickly and efficiently dissociate tissue samples into cell suspensions or cell masses for the construction of organoids. The tissue digestive fluid is widely applicable to the digestive dissociation of mouse intestine in vitro primary culture.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C with a shelf life of 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100mL","offer_id":43224141856843,"sku":"abs9644-100mL","price":160.0,"currency_code":"USD","in_stock":true},{"title":"500mL","offer_id":43224141889611,"sku":"abs9644-500mL","price":714.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.antbioinc.com\/products\/mouse-tumor-tissue-digestion-solution-abs9644","provider":"AntBio","version":"1.0","type":"link"}