{"product_id":"monkey-human-lif-onestep-elisa-kit-s0c3260","title":"Monkey \u0026 Human LIF OneStep ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAntigen\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLIF\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eImmunogen\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eRecombinant Protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAntibody Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eRecombinant mAb\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHu, Mk\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurification\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eProtein A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e12 months from date of receipt \/ reconstitution, 2 to 8°C as supplied.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eKit\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eIntra-assay: 2.3%\u003cbr\u003eInter-assay: 2.9%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSample type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eCell culture supernatant; Serum; Plasma\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAssay type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eSandwich (quantitative)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e3.87 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eRange\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e31.3 pg\/mL – 2000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eCell culture supernatant (1640): 112%\u003cbr\u003eCell culture supernatant (DMEM): 111%\u003cbr\u003eHuman Serum: 105%\u003cbr\u003eHuman EDTA Plasma: 88%\u003cbr\u003eHuman Heparin Plasma: 102%\u003cbr\u003eMonkey Serum: 103%\u003cbr\u003eMonkey EDTA Plasma: 104%\u003cbr\u003eMonkey Citrate Plasma: 99%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAssay time\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e60 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan\u003eLeukemia inhibitory factor (LIF) is a pleiotropic cytokine belonging to the IL-6 family that plays essential roles in cell differentiation, proliferation, and embryo implantation. Human LIF is synthesized as a 202-amino-acid precursor protein, and recombinant human LIF is typically produced in E. coli as a monomeric, alpha-helical four-helix bundle protein. It is widely used to maintain mouse embryonic stem cells and induced pluripotent stem cells in an undifferentiated, pluripotent state. For monkey LIF, the rhesus macaque (Macaca mulatta) and cynomolgus macaque (Macaca fascicularis) orthologs have been annotated, with the crab-eating macaque sequence encoding a 202-amino-acid protein of approximately 22 kDa. Functional studies in primate models have shown that recombinant human LIF is biologically active in cynomolgus monkeys, where administration induces weight loss and thrombocytosis. In the monkey endometrium, LIF expression is upregulated during the peri-implantation period, and its downregulation is associated with impaired endometrial receptivity. Recombinant human LIF also modulates monkey endometrial stromal cell behavior in vitro through the JAK-STAT signaling pathway. Given the high sequence conservation and cross-species bioactivity between human and monkey LIF, both proteins serve as valuable tools for studying reproductive biology, stem cell pluripotency, and hematopoietic regulation in translational primate models.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1x96T","offer_id":67455364595787,"sku":"S0C3260-1x96T","price":700.0,"currency_code":"USD","in_stock":true},{"title":"5x96T","offer_id":67455364628555,"sku":"S0C3260-5x96T","price":3420.0,"currency_code":"USD","in_stock":true},{"title":"10x96T","offer_id":67455364661323,"sku":"S0C3260-10x96T","price":6670.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/fa5c3f4aa77d465ba0a707328e35c19f_5481db11-2297-4bff-9cd3-353590a3c98a.jpg?v=1794474079","url":"https:\/\/www.antbioinc.com\/products\/monkey-human-lif-onestep-elisa-kit-s0c3260","provider":"AntBio","version":"1.0","type":"link"}