{"product_id":"mo-dc-ua090052","title":"Human MO-DC Differentiation Cytokine Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\n\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\n\u003ctable style=\"width: 100%; height: auto;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 46.3319%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 53.3071%;\"\u003eHuman MO-DC Differentiation Cytokine Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 46.3319%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 53.3071%;\"\u003eLyophilized powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 46.3319%;\"\u003e\u003cstrong\u003eReconstitution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 53.3071%;\"\u003e\n\u003ctable class=\"table table-bordered\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 11.0pt;\"\u003eIL-4\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 11.0pt;\"\u003eGM-CSF\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 11.0pt;\"\u003eTNF-α\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 11.0pt;\"\u003eIL-1β\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 11.0pt;\"\u003eIL-6\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 11.0pt;\"\u003eReconstitute at 0.1-1 mg\/ml according to the size in ultrapure water after rapid centrifugation.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 11.0pt;\"\u003ePGE2\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1. Preparation of stock solution: Dissolve 1 mg of PGE2 in 100 μL of anhydrous ethanol to prepare a 10 mg\/mL stock solution. Aliquot into small single-use portions and store at -20°C. The solution remains stable for more than 6 months.\u003cbr\u003e2. Preparation of working solution:\u003cbr\u003e1) Thaw the stock solution at room temperature before use. Dilute it with an appropriate buffer (0.1 M PBS, pH 7.2) or isotonic salt solution to prepare the working solution. Ensure that the residual amount of organic solvent does not affect the experiment, as even a small amount of organic reagent may have physiological effects on the research subject.\u003cbr\u003e[Note]: PGE2 is generally unstable in aqueous acidic or alkaline solutions. Its stability is best in solutions with a pH of 6-7.\u003cbr\u003e2) If a completely organic solvent-free PGE2 aqueous solution is required, it can be directly dissolved in PBS, pH 7.2, with a solubility of approximately 5 mg\/mL.\u003cbr\u003e[Note]: Avoid dissolving PGE2 in alkaline solutions (pH \u0026gt; 7.4), as alkali can cause PGE2 to degrade into a mixture of PGA and PGB. It is recommended to store this aqueous solution in the dark for no more than 1 day at 2-8°C. The preparation of PGE2 aqueous solutions may be challenging. The solubility can be improved or accelerated by vigorous shaking or ultrasonic treatment. Do not use heating to aid dissolution.\u003cbr\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 46.3319%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 53.3071%;\"\u003e\n\u003ctable class=\"table table-bordered\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 11.0pt;\"\u003eIL-4\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 11.0pt;\"\u003eGM-CSF\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 11.0pt;\"\u003eTNF-α\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 11.0pt;\"\u003eIL-1β\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 11.0pt;\"\u003eIL-6\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 11.0pt;\"\u003e·12 months from date of receipt, lyophilized powder stored at -20 to -80℃. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 11.0pt;\"\u003e· 3 months, -20 to -80℃ under sterile conditions after reconstitution. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 11.0pt;\"\u003e· 1 week, 2 to 8℃ under sterile conditions after reconstitution. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 11.0pt;\"\u003e· Please avoid repeated freeze-thaw cycles.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 11.0pt;\"\u003ePGE2\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 11.0pt;\"\u003e-20 °C for 12 months under sterile conditions; \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 11.0pt;\"\u003ePlease avoid repeated freeze-thaw cycles.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch4\u003eComponents\u003c\/h4\u003e\n\u003cdiv\u003e\n\u003cfigure class=\"table\"\u003e\n\u003ctable style=\"border-collapse: collapse; border: none; width: 502.5pt;\" border=\"1\" cellspacing=\"0\" cellpadding=\"0\" width=\"670\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 16.5pt;\"\u003e\n\u003ctd style=\"border: solid windowtext 1.0pt; height: 16.5pt; padding: 0cm 5.4pt 0cm 5.4pt; width: 155.5pt;\" width=\"207\" nowrap\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"color: black;\"\u003e\u003cspan style=\"font-family: '微软雅黑',sans-serif; font-size: 11.0pt;\" lang=\"EN-US\"\u003eComponent\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-left: none; border: solid windowtext 1.0pt; height: 16.5pt; padding: 0cm 5.4pt 0cm 5.4pt; width: 119.0pt;\" width=\"159\" nowrap\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"color: black;\"\u003e\u003cspan style=\"font-family: '微软雅黑',sans-serif; font-size: 11.0pt;\" lang=\"EN-US\"\u003eReference dosage\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-left: none; border: solid windowtext 1.0pt; height: 16.5pt; padding: 0cm 5.4pt 0cm 5.4pt; width: 109.5pt;\" width=\"146\" nowrap\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"color: black;\"\u003e\u003cspan style=\"font-family: '微软雅黑',sans-serif; font-size: 11.0pt;\" lang=\"EN-US\"\u003eS Size(100ml System)\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-left: none; border: solid windowtext 1.0pt; height: 16.5pt; padding: 0cm 5.4pt 0cm 5.4pt; width: 118.5pt;\" width=\"158\" nowrap\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"color: black;\"\u003e\u003cspan style=\"font-family: '微软雅黑',sans-serif; font-size: 11.0pt;\" lang=\"EN-US\"\u003eM Size(1000ml System)\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 16.5pt;\"\u003e\n\u003ctd style=\"border-top: none; border: solid windowtext 1.0pt; height: 16.5pt; padding: 0cm 5.4pt 0cm 5.4pt; width: 155.5pt;\" width=\"207\" nowrap\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"color: black;\"\u003e\u003cspan style=\"font-family: '微软雅黑',sans-serif; font-size: 11.0pt;\" lang=\"EN-US\"\u003eIL-4 Protein, Human\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom: solid windowtext 1.0pt; border-left: none; border-right: solid windowtext 1.0pt; border-top: none; height: 16.5pt; padding: 0cm 5.4pt 0cm 5.4pt; width: 119.0pt;\" width=\"159\" nowrap\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"color: black;\"\u003e\u003cspan style=\"font-family: '微软雅黑',sans-serif; font-size: 11.0pt;\" lang=\"EN-US\"\u003e50ng\/mL\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom: solid windowtext 1.0pt; border-left: none; border-right: solid windowtext 1.0pt; border-top: none; height: 16.5pt; padding: 0cm 5.4pt 0cm 5.4pt; width: 109.5pt;\" width=\"146\" nowrap\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"color: black;\"\u003e\u003cspan style=\"font-family: '微软雅黑',sans-serif; font-size: 11.0pt;\" lang=\"EN-US\"\u003e5\u003c\/span\u003e\u003cspan style=\"font-family: '微软雅黑',sans-serif; font-size: 11.0pt;\"\u003eμ\u003c\/span\u003e\u003cspan style=\"font-family: '微软雅黑',sans-serif; font-size: 11.0pt;\" lang=\"EN-US\"\u003eg\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom: solid windowtext 1.0pt; border-left: none; border-right: solid windowtext 1.0pt; border-top: none; height: 16.5pt; padding: 0cm 5.4pt 0cm 5.4pt; width: 118.5pt;\" width=\"158\" nowrap\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"color: black;\"\u003e\u003cspan style=\"font-family: '微软雅黑',sans-serif; font-size: 11.0pt;\" lang=\"EN-US\"\u003e50\u003c\/span\u003e\u003cspan style=\"font-family: '微软雅黑',sans-serif; font-size: 11.0pt;\"\u003eμ\u003c\/span\u003e\u003cspan style=\"font-family: '微软雅黑',sans-serif; font-size: 11.0pt;\" lang=\"EN-US\"\u003eg\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 16.5pt;\"\u003e\n\u003ctd style=\"border-top: none; border: solid windowtext 1.0pt; height: 16.5pt; padding: 0cm 5.4pt 0cm 5.4pt; width: 155.5pt;\" width=\"207\" nowrap\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"color: black;\"\u003e\u003cspan style=\"font-family: '微软雅黑',sans-serif; font-size: 11.0pt;\" lang=\"EN-US\"\u003eGM-CSF Protein, Human\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom: solid windowtext 1.0pt; border-left: none; border-right: solid windowtext 1.0pt; border-top: none; height: 16.5pt; padding: 0cm 5.4pt 0cm 5.4pt; width: 119.0pt;\" width=\"159\" nowrap\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"color: black;\"\u003e\u003cspan style=\"font-family: '微软雅黑',sans-serif; font-size: 11.0pt;\" lang=\"EN-US\"\u003e100ng\/mL\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom: solid windowtext 1.0pt; border-left: none; border-right: solid windowtext 1.0pt; border-top: none; height: 16.5pt; padding: 0cm 5.4pt 0cm 5.4pt; width: 109.5pt;\" width=\"146\" nowrap\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"color: black;\"\u003e\u003cspan style=\"font-family: '微软雅黑',sans-serif; font-size: 11.0pt;\" lang=\"EN-US\"\u003e10\u003c\/span\u003e\u003cspan style=\"font-family: '微软雅黑',sans-serif; font-size: 11.0pt;\"\u003eμ\u003c\/span\u003e\u003cspan style=\"font-family: '微软雅黑',sans-serif; font-size: 11.0pt;\" lang=\"EN-US\"\u003eg\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom: solid windowtext 1.0pt; border-left: none; border-right: solid windowtext 1.0pt; border-top: none; height: 16.5pt; padding: 0cm 5.4pt 0cm 5.4pt; width: 118.5pt;\" width=\"158\" nowrap\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"color: black;\"\u003e\u003cspan style=\"font-family: '微软雅黑',sans-serif; font-size: 11.0pt;\" lang=\"EN-US\"\u003e100\u003c\/span\u003e\u003cspan style=\"font-family: '微软雅黑',sans-serif; font-size: 11.0pt;\"\u003eμ\u003c\/span\u003e\u003cspan style=\"font-family: '微软雅黑',sans-serif; font-size: 11.0pt;\" lang=\"EN-US\"\u003eg\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 16.5pt;\"\u003e\n\u003ctd style=\"border-top: none; border: solid windowtext 1.0pt; height: 16.5pt; padding: 0cm 5.4pt 0cm 5.4pt; width: 155.5pt;\" width=\"207\" nowrap\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"color: black;\"\u003e\u003cspan style=\"font-family: '微软雅黑',sans-serif; font-size: 11.0pt;\" lang=\"EN-US\"\u003eTNF-\u003c\/span\u003e\u003cspan style=\"font-family: '微软雅黑',sans-serif; font-size: 11.0pt;\"\u003eα\u003c\/span\u003e\u003cspan style=\"font-family: '微软雅黑',sans-serif; font-size: 11.0pt;\" lang=\"EN-US\"\u003e Protein, Human\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom: solid windowtext 1.0pt; border-left: none; border-right: solid windowtext 1.0pt; border-top: none; height: 16.5pt; padding: 0cm 5.4pt 0cm 5.4pt; width: 119.0pt;\" width=\"159\" nowrap\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"color: black;\"\u003e\u003cspan style=\"font-family: '微软雅黑',sans-serif; font-size: 11.0pt;\" lang=\"EN-US\"\u003e10ng\/mL\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom: solid windowtext 1.0pt; border-left: none; border-right: solid windowtext 1.0pt; border-top: none; height: 16.5pt; padding: 0cm 5.4pt 0cm 5.4pt; width: 109.5pt;\" width=\"146\" nowrap\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"color: black;\"\u003e\u003cspan style=\"font-family: '微软雅黑',sans-serif; font-size: 11.0pt;\" lang=\"EN-US\"\u003e5\u003c\/span\u003e\u003cspan style=\"font-family: '微软雅黑',sans-serif; font-size: 11.0pt;\"\u003eμ\u003c\/span\u003e\u003cspan style=\"font-family: '微软雅黑',sans-serif; font-size: 11.0pt;\" lang=\"EN-US\"\u003eg\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom: solid windowtext 1.0pt; border-left: none; border-right: solid windowtext 1.0pt; border-top: none; height: 16.5pt; padding: 0cm 5.4pt 0cm 5.4pt; width: 118.5pt;\" width=\"158\" nowrap\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"color: black;\"\u003e\u003cspan style=\"font-family: '微软雅黑',sans-serif; font-size: 11.0pt;\" lang=\"EN-US\"\u003e10\u003c\/span\u003e\u003cspan style=\"font-family: '微软雅黑',sans-serif; font-size: 11.0pt;\"\u003eμ\u003c\/span\u003e\u003cspan style=\"font-family: '微软雅黑',sans-serif; font-size: 11.0pt;\" lang=\"EN-US\"\u003eg\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 16.5pt;\"\u003e\n\u003ctd style=\"border-top: none; border: solid windowtext 1.0pt; height: 16.5pt; padding: 0cm 5.4pt 0cm 5.4pt; width: 155.5pt;\" width=\"207\" nowrap\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"color: black;\"\u003e\u003cspan style=\"font-family: '微软雅黑',sans-serif; font-size: 11.0pt;\" lang=\"EN-US\"\u003eIL-1\u003c\/span\u003e\u003cspan style=\"font-family: '微软雅黑',sans-serif; font-size: 11.0pt;\"\u003eβ\u003c\/span\u003e\u003cspan style=\"font-family: '微软雅黑',sans-serif; font-size: 11.0pt;\" lang=\"EN-US\"\u003e Protein, Human\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom: solid windowtext 1.0pt; border-left: none; border-right: solid windowtext 1.0pt; border-top: none; height: 16.5pt; padding: 0cm 5.4pt 0cm 5.4pt; width: 119.0pt;\" width=\"159\" nowrap\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"color: black;\"\u003e\u003cspan style=\"font-family: '微软雅黑',sans-serif; font-size: 11.0pt;\" lang=\"EN-US\"\u003e10ng\/mL\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom: solid windowtext 1.0pt; border-left: none; border-right: solid windowtext 1.0pt; border-top: none; height: 16.5pt; padding: 0cm 5.4pt 0cm 5.4pt; width: 109.5pt;\" width=\"146\" nowrap\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"color: black;\"\u003e\u003cspan style=\"font-family: '微软雅黑',sans-serif; font-size: 11.0pt;\" lang=\"EN-US\"\u003e5\u003c\/span\u003e\u003cspan style=\"font-family: '微软雅黑',sans-serif; font-size: 11.0pt;\"\u003eμ\u003c\/span\u003e\u003cspan style=\"font-family: '微软雅黑',sans-serif; font-size: 11.0pt;\" lang=\"EN-US\"\u003eg\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom: solid windowtext 1.0pt; border-left: none; border-right: solid windowtext 1.0pt; border-top: none; height: 16.5pt; padding: 0cm 5.4pt 0cm 5.4pt; width: 118.5pt;\" width=\"158\" nowrap\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"color: black;\"\u003e\u003cspan style=\"font-family: '微软雅黑',sans-serif; font-size: 11.0pt;\" lang=\"EN-US\"\u003e10\u003c\/span\u003e\u003cspan style=\"font-family: '微软雅黑',sans-serif; font-size: 11.0pt;\"\u003eμ\u003c\/span\u003e\u003cspan style=\"font-family: '微软雅黑',sans-serif; font-size: 11.0pt;\" lang=\"EN-US\"\u003eg\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 16.5pt;\"\u003e\n\u003ctd style=\"border-top: none; border: solid windowtext 1.0pt; height: 16.5pt; padding: 0cm 5.4pt 0cm 5.4pt; width: 155.5pt;\" width=\"207\" nowrap\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"color: black;\"\u003e\u003cspan style=\"font-family: '微软雅黑',sans-serif; font-size: 11.0pt;\" lang=\"EN-US\"\u003eIL-6 Protein, Human\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom: solid windowtext 1.0pt; border-left: none; border-right: solid windowtext 1.0pt; border-top: none; height: 16.5pt; padding: 0cm 5.4pt 0cm 5.4pt; width: 119.0pt;\" width=\"159\" nowrap\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"color: black;\"\u003e\u003cspan style=\"font-family: '微软雅黑',sans-serif; font-size: 11.0pt;\" lang=\"EN-US\"\u003e50ng\/mL\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom: solid windowtext 1.0pt; border-left: none; border-right: solid windowtext 1.0pt; border-top: none; height: 16.5pt; padding: 0cm 5.4pt 0cm 5.4pt; width: 109.5pt;\" width=\"146\" nowrap\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"color: black;\"\u003e\u003cspan style=\"font-family: '微软雅黑',sans-serif; font-size: 11.0pt;\" lang=\"EN-US\"\u003e5\u003c\/span\u003e\u003cspan style=\"font-family: '微软雅黑',sans-serif; font-size: 11.0pt;\"\u003eμ\u003c\/span\u003e\u003cspan style=\"font-family: '微软雅黑',sans-serif; font-size: 11.0pt;\" lang=\"EN-US\"\u003eg\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom: solid windowtext 1.0pt; border-left: none; border-right: solid windowtext 1.0pt; border-top: none; height: 16.5pt; padding: 0cm 5.4pt 0cm 5.4pt; width: 118.5pt;\" width=\"158\" nowrap\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"color: black;\"\u003e\u003cspan style=\"font-family: '微软雅黑',sans-serif; font-size: 11.0pt;\" lang=\"EN-US\"\u003e50\u003c\/span\u003e\u003cspan style=\"font-family: '微软雅黑',sans-serif; font-size: 11.0pt;\"\u003eμ\u003c\/span\u003e\u003cspan style=\"font-family: '微软雅黑',sans-serif; font-size: 11.0pt;\" lang=\"EN-US\"\u003eg\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 16.5pt;\"\u003e\n\u003ctd style=\"border-top: none; border: solid windowtext 1.0pt; height: 16.5pt; padding: 0cm 5.4pt 0cm 5.4pt; width: 155.5pt;\" width=\"207\" nowrap\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"color: black;\"\u003e\u003cspan style=\"font-family: '微软雅黑',sans-serif; font-size: 11.0pt;\" lang=\"EN-US\"\u003ePGE2\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom: solid windowtext 1.0pt; border-left: none; border-right: solid windowtext 1.0pt; border-top: none; height: 16.5pt; padding: 0cm 5.4pt 0cm 5.4pt; width: 119.0pt;\" width=\"159\" nowrap\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"color: black;\"\u003e\u003cspan style=\"font-family: '微软雅黑',sans-serif; font-size: 11.0pt;\" lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-family: '微软雅黑',sans-serif; font-size: 11.0pt;\"\u003eμ\u003c\/span\u003e\u003cspan style=\"font-family: '微软雅黑',sans-serif; font-size: 11.0pt;\" lang=\"EN-US\"\u003eg\/mL\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom: solid windowtext 1.0pt; border-left: none; border-right: solid windowtext 1.0pt; border-top: none; height: 16.5pt; padding: 0cm 5.4pt 0cm 5.4pt; width: 109.5pt;\" width=\"146\" nowrap\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"color: black;\"\u003e\u003cspan style=\"font-family: '微软雅黑',sans-serif; font-size: 11.0pt;\" lang=\"EN-US\"\u003e1mg\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom: solid windowtext 1.0pt; border-left: none; border-right: solid windowtext 1.0pt; border-top: none; height: 16.5pt; padding: 0cm 5.4pt 0cm 5.4pt; width: 118.5pt;\" width=\"158\" nowrap\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"color: black;\"\u003e\u003cspan style=\"font-family: '微软雅黑',sans-serif; font-size: 11.0pt;\" lang=\"EN-US\"\u003e1mg\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/figure\u003e\n\u003cp\u003e\u003cspan style=\"font-family: '微软雅黑',sans-serif; font-size: 11.0pt;\"\u003eNote: S Size some components will remain.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch4\u003eProtocol\u003c\/h4\u003e\n\u003cdiv\u003e\n\u003cp\u003e\u003cspan lang=\"EN-US\"\u003e\u003cstrong\u003ePreparation of Human Peripheral Blood Monocyte-Derived Dendritic Cells\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan lang=\"EN-US\"\u003e\u003cstrong\u003e1. CD14\u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"font-family: 'MS Gothic';\"\u003e\u003cstrong\u003e⁺\u003c\/strong\u003e\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e\u003cstrong\u003e Monocyte Isolation\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan lang=\"EN-US\"\u003e1.1 Thaw cryopreserved PBMCs and centrifuge at 300 × g for 5 minutes at room temperature. Discard the supernatant and resuspend the cells in complete RPMI 1640 medium (supplemented with 10% FBS and 1% penicillin-streptomycin). Perform cell counting and viability assessment (viability should be \u0026gt;95%). Isolate CD14\u003c\/span\u003e\u003cspan style=\"font-family: 'MS Gothic';\"\u003e⁺\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e cells using CD14 positive selection magnetic beads (CD14 Nanobeads, human, Cat. No. S0K0004) according to the manufacturer's protocol. After isolation, assess the purity of the CD14\u003c\/span\u003e\u003cspan style=\"font-family: 'MS Gothic';\"\u003e⁺\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e cell population by flow cytometry. To ensure the quality of subsequent experiments, the purity should be \u0026gt;95%.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan lang=\"EN-US\"\u003e\u003cstrong\u003e2. Induction of Monocytes into Immature Dendritic Cells\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan lang=\"EN-US\"\u003e2.1 Adjust the concentration of the isolated CD14\u003c\/span\u003e\u003cspan style=\"font-family: 'MS Gothic';\"\u003e⁺\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e cells to 1\u003c\/span\u003e\u003cspan\u003e–\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e2 \u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e 10\u003c\/span\u003e\u003cspan style=\"font-family: 'MS Gothic';\"\u003e⁶\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e cells\/mL using complete RPMI 1640 medium (with 10% FBS and 1% penicillin-streptomycin). Seed 1 mL per well into a 24-well plate and immediately add the recombinant human cytokines IL-4 (50 ng\/mL) and GM-CSF (100 ng\/mL). Place the plate in a 37°C incubator with 5% CO\u003c\/span\u003e\u003cspan style=\"font-family: 'Times New Roman',serif;\" lang=\"EN-US\"\u003e₂\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e to initiate culture.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan lang=\"EN-US\"\u003e2.2 After 48 hours of culture, observe the cell morphology under a microscope. If the cells adhere well: gently aspirate half of the old medium and slowly add an equal volume of fresh complete medium containing the same concentrations of IL-4 and GM-CSF. If the cells adhere poorly or are largely in suspension: do not remove the old medium; simply supplement with an appropriate amount of fresh complete medium containing the same concentrations of cytokines.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan lang=\"EN-US\"\u003eContinue the culture: After the medium change\/supplementation, continue culturing the cells for an additional 72 hours. At the end of this period, immature dendritic cells (iDCs) will be obtained.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan lang=\"EN-US\"\u003e\u003cstrong\u003e3. Maturation of Immature Dendritic Cells into Mature Dendritic Cells\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan lang=\"EN-US\"\u003e3.1 Gently pipette to harvest the iDCs from step 2 and centrifuge at 300 × g for 10 minutes. Discard the supernatant. Resuspend the cells in fresh complete medium and adjust the density to 5 × 10\u003c\/span\u003e\u003cspan style=\"font-family: '微软雅黑',sans-serif;\"\u003e⁵\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e cells\/mL. Seed the cell suspension into a new 24-well plate and add the maturation inducer cocktail: TNF-\u003c\/span\u003e\u003cspan\u003eα\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e (10 ng\/mL), IL-1\u003c\/span\u003e\u003cspan\u003eβ\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e (10 ng\/mL), IL-6 (50 ng\/mL), and PGE\u003c\/span\u003e\u003cspan style=\"font-family: 'Times New Roman',serif;\" lang=\"EN-US\"\u003e₂\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e (1 \u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003eg\/mL). Incubate the cells at 37°C in a 5% CO\u003c\/span\u003e\u003cspan style=\"font-family: 'Times New Roman',serif;\" lang=\"EN-US\"\u003e₂\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e incubator for an additional 48 hours. Upon completion, mature dendritic cells (mDCs) will be obtained and can be used for subsequent experiments.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan lang=\"EN-US\"\u003e\u003cstrong\u003eFlow Cytometry Detection\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli style=\"tab-stops: list 36.0pt;\"\u003e\u003cspan lang=\"EN-US\"\u003e\u003cstrong\u003eCell Collection:\u003c\/strong\u003e After cytokine treatment, collect the cells.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli style=\"tab-stops: list 36.0pt;\"\u003e\n\u003cspan lang=\"EN-US\"\u003e\u003cstrong\u003eCell Counting:\u003c\/strong\u003e Count the cells and calculate the total number. Centrifuge at 300 × g for 5 minutes, discard the supernatant, and resuspend the cells in cell staining buffer (PBS with 10% FBS or 1% BSA) at a concentration of 5–10 × 10\u003c\/span\u003e\u003cspan style=\"font-family: 'MS Gothic';\"\u003e⁶\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e cells\/mL.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli style=\"tab-stops: list 36.0pt;\"\u003e\n\u003cspan lang=\"EN-US\"\u003e\u003cstrong\u003eFc Receptor Blocking:\u003c\/strong\u003e On ice, incubate 100 μL of cell suspension (containing 5–10 × 10\u003c\/span\u003e\u003cspan style=\"font-family: '微软雅黑',sans-serif;\"\u003e⁵\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e cells) with 1 \u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003eL of Human FcR Blocking Reagent (human antibodies against CD16\/32\/64, Starter Cat: S0F0012) for 30 minutes. Centrifuge at 300 \u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e g for 5 minutes and discard the supernatant.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli style=\"tab-stops: list 36.0pt;\"\u003e\n\u003cspan lang=\"EN-US\"\u003e\u003cstrong\u003eAntibody Staining:\u003c\/strong\u003e Resuspend the cells in 100 μL of PBS with 10% FBS (or 1% BSA). Add the following antibodies for single-tube staining:\u003c\/span\u003e\n\u003cul style=\"list-style-type: circle;\"\u003e\n\u003cli style=\"tab-stops: list 72.0pt;\"\u003e\u003cspan lang=\"EN-US\"\u003eAPC Rabbit Anti-Human CD80 Antibody (Starter)\u003c\/span\u003e\u003c\/li\u003e\n\u003cli style=\"tab-stops: list 72.0pt;\"\u003e\u003cspan lang=\"EN-US\"\u003eAPC Rabbit Anti-Human CD86 Antibody (Starter Cat: S0B1658)\u003c\/span\u003e\u003c\/li\u003e\n\u003cli style=\"tab-stops: list 72.0pt;\"\u003e\u003cspan lang=\"EN-US\"\u003eAnti-Hu CD83 APC\u003c\/span\u003e\u003c\/li\u003e\n\u003cli style=\"tab-stops: list 72.0pt;\"\u003e\u003cspan lang=\"EN-US\"\u003eBrilliant Violet 711™ anti-human CD11c Antibody\u003c\/span\u003e\u003c\/li\u003e\n\u003cli style=\"tab-stops: list 72.0pt;\"\u003e\u003cspan lang=\"EN-US\"\u003eBrilliant Violet 421™ anti-human CD14 Antibody\u003c\/span\u003e\u003c\/li\u003e\n\u003cli style=\"tab-stops: list 72.0pt;\"\u003e\n\u003cspan lang=\"EN-US\"\u003eAlexa Fluor® 488 anti-human HLA-DR Antibody\u003c\/span\u003e\u003cbr\u003e\u003cspan lang=\"EN-US\"\u003eIncubate at 4°C for 30 minutes. Centrifuge at 300 × g for 5 minutes and discard the supernatant.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli style=\"tab-stops: list 36.0pt;\"\u003e\u003cspan lang=\"EN-US\"\u003e\u003cstrong\u003eCell Washing:\u003c\/strong\u003e Wash the cells with PBS to remove residual antibodies, then resuspend again in PBS.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli style=\"tab-stops: list 36.0pt;\"\u003e\u003cspan lang=\"EN-US\"\u003e\u003cstrong\u003eViability Dye Staining:\u003c\/strong\u003e Add 7-AAD to each tube and incubate at room temperature in the dark for 5 minutes.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli style=\"tab-stops: list 36.0pt;\"\u003e\u003cspan lang=\"EN-US\"\u003e\u003cstrong\u003ePerform Flow Cytometry Analysis.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/div\u003e\n\u003ch4\u003eGuidelines\u003c\/h4\u003e\n\u003cdiv\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003eStore in separate containers to minimize freeze-thaw cycles.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"S","offer_id":42302183637067,"sku":null,"price":480.0,"currency_code":"USD","in_stock":true},{"title":"M","offer_id":42302183669835,"sku":null,"price":1800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/a2672868-36b8-4a3d-acde-48b89ef7a44a.jpg?v=1776073664","url":"https:\/\/www.antbioinc.com\/products\/mo-dc-ua090052","provider":"AntBio","version":"1.0","type":"link"}