{"product_id":"mihc-dye-tsa540","title":"mIHC Dye (TSA540)","description":"\u003cdiv data-page-id=\"KtGkf50pDdlIX4cKdWRc68eanvh\" data-lark-html-role=\"root\" data-docx-has-block-data=\"false\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-PiERfF1QhdgsaQcUwqhcP2cznvg\"\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/div\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-AZXLf62GqdJjDOcbCfBcvY9knhe\"\u003emIHC Dye (TSA540) is a high-performance fluorescent dye designed for multiplex immunohistochemistry (mIHC) based on tyramide signal amplification (TSA) technology. It enables robust, covalent fluorescent labeling on formalin-fixed paraffin-embedded (FFPE) tissues, frozen sections, and tissue microarray (TMA) slides, supporting multi-target, in situ biomarker detection in a single tissue section.\u003c\/div\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-VXgFf075NdOLB0cZeEjcjytXnGe\"\u003eThis reagent provides strong, stable fluorescence with Ex\/Em: 520 nm \/ 540 nm, compatible with standard fluorescence microscopes and multispectral imaging systems. It delivers greatly amplified signals compared to conventional immunofluorescence, ideal for low-abundance antigens and spatial profiling of the tissue microenvironment.\u003c\/div\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-AlfJfKwomddzpBcSEXUcCUU6nyg\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-O6QKfNuvkdtG0vcx5JdcQMQ0nfe\"\u003e\u003cstrong\u003eKey Features\u003c\/strong\u003e\u003c\/div\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-M8xBfQBrPdS47pcs0gYc2GtCnSg\"\u003e\n\u003cstrong\u003eTSA-based signal amplification\u003c\/strong\u003e: Ensures high sensitivity and high signal-to-noise ratio, suitable for detecting low-abundance antigens.\u003c\/div\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-LSYBfTm5AdtIwvc9W1fcpCnmn5b\"\u003e\n\u003cstrong\u003eCovalent binding\u003c\/strong\u003e: Binds to tyrosine residues covalently, maintaining excellent stability during antibody stripping and repeated staining cycles.\u003c\/div\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-OMw1fkMLJdOONoclCcpcmqmFnMb\"\u003e\n\u003cstrong\u003eBroad Compatibility\u003c\/strong\u003e: Applicable to FFPE tissues, frozen sections, and TMA slides, meeting diverse experimental needs.\u003c\/div\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-HPoaf1EfDdTQW5coTDPcTbL1njb\"\u003e\n\u003cstrong\u003eSpectral Characteristics\u003c\/strong\u003e: 540 nm spectral channel, matching AF514, Opal 540, and similar orange-yellow fluorophores.\u003c\/div\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-XsR2ffEWtdnCbrctKGCcR83xnfA\"\u003e\n\u003cstrong\u003eEasy to Use\u003c\/strong\u003e: Ready-to-use formulation with included signal amplification reaction buffer, no complex pre-processing required.\u003c\/div\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-Cl4efZC5yd1eUIcZMIJcYasEn2f\"\u003e\n\u003cstrong\u003eApplication Scope\u003c\/strong\u003e: For research use only; not for in vitro clinical diagnosis or human trials.\u003c\/div\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-PhTbf4dHEdSysZcIRDrcQlsKnVe\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-InstfxJCTdYNercpAzPczQfenKd\"\u003e\u003cstrong\u003ePrinciple\u003c\/strong\u003e\u003c\/div\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-Gqkdf64eSd7uXQcNO7qchyGEnXg\"\u003eTSA540 adopts horseradish peroxidase (HRP)-conjugated secondary antibodies to activate fluorescently labeled tyramide substrates. The activated tyramide rapidly forms covalent bonds with tyrosine residues near the antigen-antibody complex, forming stable fluorescent deposits. After that, the antibodies can be stripped by heat repair without removing the dye signal, allowing sequential labeling of multiple targets on the same tissue section to achieve multiplex staining.\u003c\/div\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-ZrmVfyrH5dPvRIcXZzfc9AvXnYf\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-L7TffGQ2Ydxjk3ccwuKcBX4zn0c\"\u003e\u003cstrong\u003eStorage \u0026amp; Stability\u003c\/strong\u003e\u003c\/div\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-Vpdif4J1MdIGHRchx8scTZionQf\"\u003e\n\u003cstrong\u003eStorage Condition\u003c\/strong\u003e: Store protected from light at 2–8 °C; avoid direct exposure to strong light.\u003c\/div\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-Eyswf9CCodfWg8cQluJcTIzxnHb\"\u003e\n\u003cstrong\u003eShelf Life\u003c\/strong\u003e: Stable for 12 months from the date of receipt when stored according to the required conditions.\u003c\/div\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-TiDGfoSr3dv0bgclZ5KcyiACnDG\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-LLCrfBxzAdZMLQclQ3LcnmeBnMh\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/div\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-XBfxfwRx3dLBy1cdOwLcV4YAnhe\"\u003eTSA Monochromatic Fluorescent Dye 540, Signal Amplification Reaction Buffer\u003c\/div\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-QLgAfc4g3de7epcIScdcErzln3g\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-O2sbfqE3Odxu45clVZwcUgbfnjh\"\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/div\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-Vm4KfYkpUdIrzrcDpqJcV176nEc\"\u003eMainly used for scientific research of immunohistochemical staining of tissues, paraffin sections, and TMA chips; not for in vitro clinical diagnosis or human trials.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"AntBio","offers":[{"title":"100T","offer_id":42373449318475,"sku":null,"price":498.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/antbio-absin-starter-ua_a170e2c6-4b7b-45c9-b83c-cbec40e95194.png?v=1777444660","url":"https:\/\/www.antbioinc.com\/products\/mihc-dye-tsa540","provider":"AntBio","version":"1.0","type":"link"}