{"product_id":"lat1-4f2hc-flag-his-tag-protein-human-detergent-ua060028","title":"LAT1-4F2hc Flag \u0026 His tag Protein, Human (Detergent)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLAT1-4F2hc\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAccession\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eNM_003486.7、NM_001012662.2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAmino Acid Sequence\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eLAT1: Ala2-Thr507 with Flag Tag at the N-Terminus\u003cbr\u003e4F2hc: Glu2-Ala631 with His Tag at the N-Terminus\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHEK293\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003eLAT1:35-40kDa (Reducing)\n4F2hc:90-130kDa (Reducing)\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e＞90% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e50mM Hepes, 150mM NaCl, 0.02% GDN, pH8.0\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e·6 months from date of receipt, -60 to -80 °C as supplied.\u003cbr\u003e·about 3 days in 2 to 8 C.\u003cbr\u003e·Please avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e1.Bhakuni T, Norden PR, Ujiie N, Tan C, Lee SK, Tedeschi T, Hsieh YW, Wang Y, Liu T, Fawzi AA, Kume T. FOXC1 regulates endothelial CD98 (LAT1\/4F2hc) expression in retinal angiogenesis and blood-retina barrier formation. Nat Commun. 2024 May 16;15(1):4097.\u003cbr\u003e2.Alles SRA, Gomez K, Moutal A, Khanna R. Putative roles of SLC7A5 (LAT1) transporter in pain. Neurobiol Pain. 2020 Jun 30;8:100050.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan\u003eLAT1 (SLC7A5) is an essential amino acid transporter that forms a heterodimer with 4F2hc (SLC3A2). The recently resolved cryo-EM structure reveals a critical \"outward-facing occluded conformation,\" where inhibitors bind to the classical substrate-binding pocket, with their tails specifically wedged between the substrate-binding site and transmembrane domain TM10. This provides atomic-level details for understanding its transport and inhibition mechanisms. LAT1 is not only responsible for transporting essential amino acids but also mediates the transmembrane influx of neuropathic pain drugs such as gabapentin. Additionally, by interacting with voltage-gated potassium channels (Kv1.1\/Kv1.2) and sodium channels (Nav1.7), it regulates neuronal excitability and plays a central role in nociceptive transmission. LAT1 is highly expressed in various tumors, making it a potential target for anticancer drug design. At the same time, its upregulation in pain pathways of the dorsal root ganglia and spinal cord, along with its close association with mTOR signaling, the Wnt\/β-catenin pathway, and T\/B cell-related pain, positions it not only as a novel target for alleviating neuropathic pain (e.g., by mediating the uptake of first-line drugs) but also as a key structural foundation for developing new drugs capable of crossing the blood-brain barrier while exerting analgesic effects.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"25μg","offer_id":42332766077003,"sku":null,"price":500.0,"currency_code":"USD","in_stock":true},{"title":"100μg","offer_id":42332766109771,"sku":null,"price":1500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/07bd81dd-8272-40e5-9b3b-61e8f543bfb8.png?v=1776073725","url":"https:\/\/www.antbioinc.com\/products\/lat1-4f2hc-flag-his-tag-protein-human-detergent-ua060028","provider":"AntBio","version":"1.0","type":"link"}