{"product_id":"human-treg-panel-kit-cd3cd4cd8cd25foxp3-s0n0022","title":"Human Treg Panel Kit (CD3\/CD4\/CD8\/CD25\/FOXP3)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eFCM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHu\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePositive Sample\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman PBMC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003ePBS, 1% BSA, 0.09% sodium azide\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e12 months from date of receipt \/ reconstitution, 2 to 8 °C as supplied.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan\u003eThis f\u003c\/span\u003e\u003cspan\u003eive\u003c\/span\u003e\u003cspan\u003ecolor panel is designed for detecting regulatory T cells (Tregs) in human peripheral blood or other biological samples.\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eCD3 was included as a general T\u003c\/span\u003e\u003cspan\u003e‑\u003c\/span\u003e\u003cspan\u003ecell marker, and CD4 and CD8 were included to identify helper T\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003ecell\u003c\/span\u003e\u003cspan\u003es\u003c\/span\u003e\u003cspan\u003e and cytotoxic T\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003ecell\u003c\/span\u003e\u003cspan\u003es\u003c\/span\u003e\u003cspan\u003e subpopulations. CD25 is a marker often used to detect Tregs, and FOXP3 was included as a specific transcription factor for Tregs; it is important to note that FOXP3 is an intracellular marker and requires fixation and permeabilization. Finally, Tregs were identified as CD25+FOXP3+ cells within the \u003c\/span\u003e\u003cspan\u003ehelper T\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003ecell\u003c\/span\u003e\u003cspan\u003es\u003c\/span\u003e\u003cspan\u003e gate. The panel is compatible with most three\u003c\/span\u003e\u003cspan\u003e‑\u003c\/span\u003e\u003cspan\u003elaser flow cytometers. If desired, the panel can serve as a backbone panel, allowing for the addition of antibodies conjugated to various fluorophores to detect other markers of interest.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"50T","offer_id":67374467612747,"sku":"S0N0022-50T","price":670.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/9ea5f8c3f4c7416493804e87ec8c6c5d.png?v=1793602845","url":"https:\/\/www.antbioinc.com\/products\/human-treg-panel-kit-cd3cd4cd8cd25foxp3-s0n0022","provider":"AntBio","version":"1.0","type":"link"}