{"product_id":"human-total-igg-kit-high-sensitivity-hica-ua086603","title":"Human Total IgG Kit (High Sensitivity) (HICA)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eStore at 2–8°C, protected from light, for 6 months. After reconstitution, the calibrator may be aliquoted and stored at −20°C for up to 3 months; avoid repeated freeze-thaw cycles.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003eHuman immunoglobulin\u003c\/span\u003e\u003cspan\u003eG (Human Immunoglobulin G, IgG) is an antibody secreted and released by plasma cells (B cells). It is the most common type of antibody in the bloodstream and the most abundant immunoglobulin in human serum, accounting for approximately 75% of total serum immunoglobulins. The molecular weight of IgG is about 150 kDa, and it typically exists as a tetrameric structure composed of two identical heavy chains and two identical light chains linked by interchain disulfide bonds. IgG serves as a major effector molecule in humoral immune responses. Based on differences in the amino acid composition and structure of the hinge region, IgG can be divided into four subclasses: IgG1 (approximately 66%), IgG2 (approximately 23%), IgG3 (approximately 7%), and IgG4 (approximately 4%). IgG is distributed in blood and tissue fluids, where it exerts various immune functions, including neutralizing pathogens, activating complement, opsonizing phagocytosis, and mediating antibody-dependent cell-mediated cytotoxicity. It plays a central role in anti-infection immunity and immunological memory and can cross the placenta to provide passive immune protection to newborns. Clinically, IgG is an important indicator for assessing humoral immunity, vaccine efficacy, and the mechanisms of autoimmune diseases, and it is widely used in disease mechanism research, drug efficacy evaluation, and biomarker development. This kit…\u003c\/span\u003e\u003cspan\u003eFor the quantitative in vitro detection of total [substance] in cell culture supernatants, serum, plasma, and other samples.\u003c\/span\u003e\u003cspan\u003eIgG concentration provides a sensitive, specific, and efficient tool suitable for basic medical research and drug screening applications.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003ePrinciple of the test:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/388c9c14-5c4e-419f-b71d-e246dc45cf8a.png\" style=\"margin-left:30px; width:508px; height:auto; vertical-align:middle;\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThis kit employs a homogeneous chemiluminescence assay based on the sandwich ELISA method.\u003c\/span\u003e\u003cspan\u003eHomogeneous Immuno Chemiluminescence Assay, HICA\u003c\/span\u003e\u003cspan\u003e) to detect cytokine concentrations, with simple operation,\u003c\/span\u003e\u003cspan\u003eNo washing required.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe detection system includes two types of microspheres:\u003c\/span\u003e\u003cspan\u003eDonor\u003c\/span\u003e\u003cspan\u003eMicrosphere conjugation targeting the target protein\u003c\/span\u003e\u003cspan\u003eAntibody 1\u003c\/span\u003e\u003cspan\u003e,\u003c\/span\u003e\u003cspan\u003eReceptor\u003c\/span\u003e\u003cspan\u003eMicrosphere conjugation\u003c\/span\u003e\u003cspan\u003eAntibody to the target protein 2\u003c\/span\u003e\u003cspan\u003eAt the time of the reaction, the target protein binds to the antibody and the microspheres, forming an immune complex that brings the two types of microspheres into close proximity. When the distance between the donor microsphere and the acceptor microsphere is less than 200 nm, the singlet oxygen generated upon light excitation can be transferred to the acceptor microsphere, triggering chemiluminescence; conversely, if the target protein is absent and the microspheres are too far apart, no signal is produced.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eQuantitative analysis of the target protein can be achieved by measuring the intensity of the chemiluminescent signal. This method is characterized by its simplicity, rapid reaction kinetics, and high sensitivity.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003ctable border=\"1\" style=\"border-collapse:collapse;width:auto;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#FFFFFF;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003eName\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#FFFFFF;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003eSpecification\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#FFFFFF;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003eComponent Specification\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"2\" style=\"text-align:center;vertical-align:middle;background-color:#FFFFFF;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e \u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eTest Reagent\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eR1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#FFFFFF;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000;background-color:yellow\"\u003e100 Tests\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#FFFFFF;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e6\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eμ\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003el \/\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eper tube ×\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#FFFFFF;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000;background-color:yellow\"\u003e5\u003c\/span\u003e\u003cspan style=\"color:#000000;background-color:yellow\"\u003e00 Tests\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#FFFFFF;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e3\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e0\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eμ\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003el \/\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eper tube ×\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#FFFFFF;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e \u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eTest Reagent\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eR2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#FFFFFF;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000;background-color:yellow\"\u003e100 Tests\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#FFFFFF;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e6\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eμ\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003el \/\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eper tube ×\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#FFFFFF;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#FFFFFF;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000;background-color:yellow\"\u003e50\u003c\/span\u003e\u003cspan style=\"color:#000000;background-color:yellow\"\u003e0 Tests\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#FFFFFF;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e30\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eμ\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003el \/\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eper tube ×\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#FFFFFF;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003eCalibrator\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#FFFFFF;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000;background-color:yellow\"\u003e100 Tests\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#FFFFFF;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e100 ng\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eLyophilized powder ×\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#FFFFFF;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#FFFFFF;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000;background-color:yellow\"\u003e500 Tests\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#FFFFFF;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e100 ng\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eLyophilized powder ×\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"2\" style=\"text-align:center;vertical-align:middle;background-color:#FFFFFF;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e \u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eReagent Diluent\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#FFFFFF;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000;background-color:yellow\"\u003e100 Tests\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#FFFFFF;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e2.2 mL\/\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eper bottle ×\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#FFFFFF;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000;background-color:yellow\"\u003e50\u003c\/span\u003e\u003cspan style=\"color:#000000;background-color:yellow\"\u003e0 Tests\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#FFFFFF;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e11 mL\/\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eper bottle ×\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"2\" style=\"text-align:center;vertical-align:middle;background-color:#FFFFFF;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCalibrator Diluent\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#FFFFFF;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000;background-color:yellow\"\u003e100 Tests\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#FFFFFF;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e1 mL\/\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eper tube ×\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#FFFFFF;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000;background-color:yellow\"\u003e500 Tests\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#FFFFFF;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e1 mL\/\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eper tube ×\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003eNote: It is recommended to use microplates for the assay (\u003c\/span\u003e\u003cspan style=\"font-weight:bold;background-color:yellow\"\u003e384\u003c\/span\u003e\u003cspan style=\"font-weight:bold;background-color:yellow\"\u003eshallow wells\u003c\/span\u003e\u003cspan style=\"font-weight:bold;background-color:yellow\"\u003eor\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e3\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e84\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e,\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e96\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003ewell plates, white or gray)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003eSample requirements\u003c\/span\u003e\u003cbr\u003e\u003cspan\u003e1.\u003c\/span\u003e\u003cspan\u003eTo remove impurities from the sample, the sample must be centrifuged prior to analysis.\u003c\/span\u003e\u003cspan style=\"background-color:yellow\"\u003e2\u003c\/span\u003e\u003cspan style=\"background-color:yellow\"\u003e000 ×\u003c\/span\u003e\u003cspan\u003eg\u003c\/span\u003e\u003cspan\u003e,\u003c\/span\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003eminutes).\u003c\/span\u003e\u003cbr\u003e\u003cspan\u003e2.\u003c\/span\u003e\u003cspan\u003eIf the measured concentration of a sample exceeds the highest value on the standard curve, it should be appropriately diluted (using negative serum is recommended) and retested to ensure that the result falls within the valid measurement range.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003eTesting process\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003e2\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e.1\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003ePreparation of calibration standard gradient samples\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eAdopt 10\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e0\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003emu\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eL\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eReconstitute with deionized water.\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e100\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eThe lyophilized calibrator is designated as C.\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e12\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e, then dilute the reconstituted lyophilized calibrator C with the calibrator diluent.\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e12\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003ePerform gradient dilution, calibrator C.\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e1\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eThis is the calibrator diluent; the recommended dilution protocol is shown in the table below:\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse:collapse;width:auto;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#D8D8D8;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eGradient\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#D8D8D8;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003eConcentration (pg\/mL)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#D8D8D8;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003eHigher-level school\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eQualified product\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e(microliters)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#D8D8D8;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003eDiluent (μL)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#333333\"\u003eC12\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#333333\"\u003e1,000,000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#333333\"\u003e-\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#333333\"\u003e-\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#333333\"\u003eC11\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#333333\"\u003e300,000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#333333\"\u003e30\u003c\/span\u003e\u003cspan style=\"color:#333333\"\u003eμL C1\u003c\/span\u003e\u003cspan style=\"color:#333333\"\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#333333\"\u003e70\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#333333\"\u003eC10\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#333333\"\u003e100000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#333333\"\u003e30\u003c\/span\u003e\u003cspan style=\"color:#333333\"\u003eμL C1\u003c\/span\u003e\u003cspan style=\"color:#333333\"\u003e1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#333333\"\u003e60\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#333333\"\u003eC9\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#333333\"\u003e30000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#333333\"\u003e30\u003c\/span\u003e\u003cspan style=\"color:#333333\"\u003eμL C\u003c\/span\u003e\u003cspan style=\"color:#333333\"\u003e10\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#333333\"\u003e70\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#333333\"\u003eC8\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#333333\"\u003e10000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#333333\"\u003e30\u003c\/span\u003e\u003cspan style=\"color:#333333\"\u003eμL C\u003c\/span\u003e\u003cspan style=\"color:#333333\"\u003e9\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#333333\"\u003e60\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#333333\"\u003eC7\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#333333\"\u003e3000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#333333\"\u003e30\u003c\/span\u003e\u003cspan style=\"color:#333333\"\u003eμL C\u003c\/span\u003e\u003cspan style=\"color:#333333\"\u003e8\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#333333\"\u003e70\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#333333\"\u003eC6\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#333333\"\u003e1000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#333333\"\u003e30\u003c\/span\u003e\u003cspan style=\"color:#333333\"\u003eμL C\u003c\/span\u003e\u003cspan style=\"color:#333333\"\u003e7\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#333333\"\u003e60\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#333333\"\u003eC5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#333333\"\u003e300\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#333333\"\u003e30\u003c\/span\u003e\u003cspan style=\"color:#333333\"\u003eμL C\u003c\/span\u003e\u003cspan style=\"color:#333333\"\u003e6\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#333333\"\u003e70\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#333333\"\u003eC4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#333333\"\u003e100\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#333333\"\u003e30\u003c\/span\u003e\u003cspan style=\"color:#333333\"\u003eμL C\u003c\/span\u003e\u003cspan style=\"color:#333333\"\u003e5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#333333\"\u003e60\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#333333\"\u003eC3\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#333333\"\u003e30\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#333333\"\u003e30\u003c\/span\u003e\u003cspan style=\"color:#333333\"\u003eμL C\u003c\/span\u003e\u003cspan style=\"color:#333333\"\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#333333\"\u003e70\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#333333\"\u003eC2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#333333\"\u003e10\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#333333\"\u003e30μL C3\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#333333\"\u003e60\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#333333\"\u003eC1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#333333\"\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003e2.2\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003eTesting procedure:\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse:collapse;width:auto;border:1px solid #000000\"\u003e\n\u003ctr\u003e\u003ctd colspan=\"2\" style=\"text-align:center;vertical-align:middle;background-color:#EDEDED;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003eTesting procedure\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003e \u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003e25\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003e℃\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003eOne-step method\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003eTesting\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#FFFFFF;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e​\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003eSample addition\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#FFFFFF;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000;background-color:yellow\"\u003e1\u003c\/span\u003e\u003cspan style=\"color:#000000;background-color:yellow\"\u003e.\u003c\/span\u003e\u003cspan style=\"color:#000000;background-color:yellow\"\u003eTake\u003c\/span\u003e\u003cspan style=\"color:#000000;background-color:yellow\"\u003e2\u003c\/span\u003e\u003cspan style=\"color:#000000;background-color:yellow\"\u003eµL of sample\u003c\/span\u003e\u003cspan style=\"color:#000000;background-color:yellow\"\u003eAdd to\u003c\/span\u003e\u003cspan style=\"color:#000000;background-color:yellow\"\u003e384\u003c\/span\u003e\u003cspan style=\"color:#000000;background-color:yellow\"\u003emicroplate wells;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e2\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e.\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eUltrasonicate\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eR1\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eand\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eR2\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003efor\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e5 seconds\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eAfter vortexing or mixing, mix equal volumes and homogenize, then dilute with the reagent diluent.\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e200\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003efold and mix to prepare the working reagent solution;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e3\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e.\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eAdd to the sample\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eadd\u003c\/span\u003e\u003cspan style=\"color:#000000;background-color:yellow\"\u003e18\u003c\/span\u003e\u003cspan style=\"color:#000000;background-color:yellow\"\u003eµL of the premixed\u003c\/span\u003e\u003cspan style=\"color:#000000;background-color:yellow\"\u003eworking reagent solution\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#FFFFFF;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003eincubate\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#FFFFFF;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e​\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eon a microplate shaker\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e8\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e00 r\/min\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e25\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e°\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eC\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eincubate\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e60\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003emin\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#FFFFFF;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eread the results\u003c\/span\u003e\u003cspan\u003e​\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#FFFFFF;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003einstrument reading,\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#00B050\"\u003eprotect from light\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#00B050\"\u003e\/green light\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan\u003eNote:\u003c\/span\u003e\u003cspan\u003ethe diluted\u003c\/span\u003e\u003cspan\u003eR1\u003c\/span\u003e\u003cspan\u003eand\u003c\/span\u003e\u003cspan\u003eR2\u003c\/span\u003e\u003cspan\u003eshould be used within\u003c\/span\u003e\u003cspan\u003ehalf\u003c\/span\u003e\u003cspan\u003ean hour after mixing\u003c\/span\u003e\u003cspan\u003e, and must be\u003c\/span\u003e\u003cspan style=\"color:#00B050\"\u003eprotected from light\u003c\/span\u003e\u003cspan style=\"color:#00B050\"\u003e\/green light\u003c\/span\u003e\u003cspan\u003e;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"background-color:yellow\"\u003ethe reaction system can be adjusted proportionally according to experimental needs.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan style=\"color:#000000\"\u003eIII. Performance Testing\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003e3\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e.1\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eExample of a complete standard curve:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/2ca6a498-8375-4c30-98aa-4d83636b9d02.png\" style=\"margin-left:30px; width:297px; height:auto; vertical-align:middle;\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/ff359937-bc2c-4f79-a02e-72890ee448a3.png\" style=\"margin-left:30px; width:314px; height:auto; vertical-align:middle;\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003e3\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e.2\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003ePerformance parameter verification:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003eLOB\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003e:\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003erepeat the detection\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e20\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003etimes for the calibration standard\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eC1\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e, calculate the mean signal and\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eSD\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e, use the standard curve to calculate the concentration value corresponding to\u003c\/span\u003e\u003cspan style=\"text-decoration:underline;color:#000000\"\u003emean signal\u003c\/span\u003e\u003cspan style=\"text-decoration:underline;color:#000000\"\u003e+2\u003c\/span\u003e\u003cspan style=\"text-decoration:underline;color:#000000\"\u003e×\u003c\/span\u003e\u003cspan style=\"text-decoration:underline;color:#000000\"\u003eSD\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e, which is the limit of detection LO\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eB\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003ematrix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eblank limit\u003c\/span\u003e\u003cspan\u003e(pg\/mL)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003ebuffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e7.0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003edynamic range (\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003eDynamic range):\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003e0 –\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003e00\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e0\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003e000 pg\/mL.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003eLinear range (\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003eLinear Range):\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e100\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003e– 10\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e0\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003e000 pg\/mL.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003ePrecision (\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003ePrecision\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003e)\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e:\u003c\/span\u003e\u003cbr\u003e\u003cspan style=\"color:#000000\"\u003eIntra-precision\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e-\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003ePrecision): In the same experiment\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eOn the orifice plate, using known low,\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eChina,\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eEach high-concentration sample was tested in triplicate.\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e1\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eZero times, to evaluate the intra-assay precision of this assay.\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eschool\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eBoth the reference product and the sample\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eTest using different processes.\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eThe coefficient of variation (%CV) for the test results of samples at each concentration was less than 10%, indicating that the method exhibits good reproducibility within the same batch.\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"2\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eMatrix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003ePrecision\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003elow\u003c\/span\u003e\u003cspan\u003eConcentration\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eMedium concentration\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eHigh concentration\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eBuffer solution\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003cspan\u003e%\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3%\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1%\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cbr\u003e\u003cspan style=\"color:#000000\"\u003eInter-plate precision (Inter\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e-\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003ePrecision): In 5\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eIn the next independent experiment, known low levels were tested separately,\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eChina,\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eHigh-concentration samples are being tested. Settings\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e5\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003erepeated times. The test results of samples at each concentration all showed a low coefficient of variation (CV \u0026lt; 10%), indicating that the method has good reproducibility between different batches.\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"2\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eMatrix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIntermediate precision\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eLow concentration\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eMedium concentration\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eHigh concentration\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eBuffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e8\u003c\/span\u003e\u003cspan\u003e%\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5%\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4%\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003eAccuracy (\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003eRecovery\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003e):\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003eStandard solutions of IgG at low, medium, and high concentrations were added to different matrices, and their average recovery rates were tested twice.\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eIgG\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eMatrices\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"2\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eRecovery rate\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eLow concentration\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eMedium concentration\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eHigh concentration\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCalibrator diluent\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e97%\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e91%\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e95%\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCulture supernatant\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e87%\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e97%\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e95%\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e200X\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eMouse serum\u003c\/span\u003e\u003cspan\u003e95\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e%\u003c\/span\u003e\u003cspan\u003e93%\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e94%\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e200X\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eMouse plasma\u003c\/span\u003e\u003cspan\u003e91%\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e95%\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e95%\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e95%\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e200X\u003c\/span\u003e\u003cspan\u003eRat serum\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e85%\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e95%\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e91%\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/11d8eeee-d0fe-4f4b-9ddb-625d038b8ddd.jpeg\" style=\"margin-left:30px; width:453px; height:auto; vertical-align:middle;\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003eDilution linearity (\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003eDilution linearity\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003e):\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003eThe samples were diluted by factors of 8\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eThe serum and plasma were diluted in a gradient after being multiplied by a factor, and corrections were applied; additionally, the supernatant of the culture medium, calibrator diluent, and dilutions were used.\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eSerum and plasma after 80,000-fold dilution\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eAdd to the middle\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eIgG\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eThe standard was serially diluted and used for calibration to evaluate its dilution linearity.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/0f91eb2b-9591-4281-b680-d0c02cb143b1.png\" style=\"margin-left:30px; width:388px; height:auto; vertical-align:middle;\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/6b32d74d-e785-490c-bd8b-69f1cd7634e6.jpeg\" style=\"margin-left:30px; width:431px; height:auto; vertical-align:middle;\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/558b1d38-230e-47e8-b89b-3385d6ed18fb.png\" style=\"margin-left:30px; width:508px; height:auto; vertical-align:middle;\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003eHook effect (\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003eHOOK\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003e):\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003etake\u003c\/span\u003e\u003cspan\u003eI\u003c\/span\u003e\u003cspan\u003egG\u003c\/span\u003e\u003cspan\u003eStandard substance\u003c\/span\u003e\u003cspan\u003eGradient dilution and testing confirmed that samples within the measurement range yield reliable quantitative results, and no signal suppression was observed.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/e265cab8-8817-420a-bc96-fcffef9ff72e.png\" style=\"margin-left:30px; width:508px; height:auto; vertical-align:middle;\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003eSpecificity (\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003eSpecificity:):\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eDilute sera and plasmas from different species separately.\u003c\/span\u003e\u003cspan\u003eAfter 80,000-fold dilution, the results showed that this kit exhibited no cross-reactivity in the following species.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/6201c4b3-02db-4026-853f-e0d395f0b5dd.png\" style=\"margin-left:30px; width:426px; height:auto; vertical-align:middle;\"\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eGuidelines\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003eReagents R1 and R2 should be used protected from light; sample addition and incubation are recommended to be performed under green light or dim light (100 LUX).\u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003eEach assay requires recalibration, with each concentration level of the calibrator tested in at least duplicate wells, using five-parameter curve fitting for calculation.\u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003eDuring incubation, temperature and time must be strictly controlled; it is recommended to cover the microplate with a film, and an ELISA reader with ALPHA functionality should be used.\u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003eComponents from different lot numbers of the kit must not be mixed.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"100T","offer_id":67240572485707,"sku":"UA086603-100T","price":420.0,"currency_code":"USD","in_stock":true},{"title":"500T","offer_id":67240572518475,"sku":"UA086603-500T","price":1600.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_939e8d1f-19ab-4996-8e71-37be6e7e5246.png?v=1792011706","url":"https:\/\/www.antbioinc.com\/products\/human-total-igg-kit-high-sensitivity-hica-ua086603","provider":"AntBio","version":"1.0","type":"link"}