{"product_id":"human-th1th2th17-cell-panel-kit-s0n0025","title":"Human TH1\/TH2\/TH17 Cell Panel Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eFCM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHu\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePositive Sample\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eTh1, Th2, Th17 polarized human PBMC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003ePBS, 1% BSA, 0.09% sodium azide\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e12 months from date of receipt \/ reconstitution, 2 to 8 °C as supplied.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan\u003eThis f\u003c\/span\u003e\u003cspan\u003eive\u003c\/span\u003e\u003cspan\u003e‑\u003c\/span\u003e\u003cspan\u003ecolor panel is designed for detecting T helper subsets, including Th1, Th2, and Th17 cells, \u003c\/span\u003e\u003cspan\u003ehuman peripheral blood or other biological samples\u003c\/span\u003e\u003cspan\u003e. CD3 was included as a general T\u003c\/span\u003e\u003cspan\u003e‑\u003c\/span\u003e\u003cspan\u003ecell marker, and CD4 was included to identify helper T cells (CD4+). Intracellular cytokine staining for IFN\u003c\/span\u003e\u003cspan\u003e‑\u003c\/span\u003e\u003cspan\u003eγ, IL\u003c\/span\u003e\u003cspan\u003e‑\u003c\/span\u003e\u003cspan\u003e4, and IL\u003c\/span\u003e\u003cspan\u003e‑\u003c\/span\u003e\u003cspan\u003e17A was used to identify Th1 (IFN\u003c\/span\u003e\u003cspan\u003e‑\u003c\/span\u003e\u003cspan\u003eγ+), Th2 (IL\u003c\/span\u003e\u003cspan\u003e‑\u003c\/span\u003e\u003cspan\u003e4+), and Th17 (IL\u003c\/span\u003e\u003cspan\u003e‑\u003c\/span\u003e\u003cspan\u003e17A+) subsets; it is important to note that these cytokines are intracellular markers and require fixation and permeabilization before staining. The panel is compatible with most three\u003c\/span\u003e\u003cspan\u003e‑\u003c\/span\u003e\u003cspan\u003elaser flow cytometers. If desired, the panel can serve as a backbone panel, allowing for the addition of antibodies conjugated to various fluorophores to detect other markers of interest.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"50T","offer_id":67167973867595,"sku":"S0N0025-50T","price":670.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/f46df53c991744deabdd7d2e6d48c5f9.png?v=1790794811","url":"https:\/\/www.antbioinc.com\/products\/human-th1th2th17-cell-panel-kit-s0n0025","provider":"AntBio","version":"1.0","type":"link"}