{"product_id":"human-igg-kit-hica-ua086034","title":"Human IgG Kit (HICA)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cstrong style=\"font-weight: 600\"\u003eTesting Principle:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis kit employs the Homogeneous Immuno Chemiluminescence Assay (HICA), a highly sensitive homogeneous detection technology based on beads. This technology utilizes two types of beads: donor beads and acceptor beads. When molecules attached to the donor beads interact with molecules attached to the acceptor beads, and the two beads come into sufficiently close proximity (\u0026lt;200 nm), a luminescent signal can be detected.\u003c\/p\u003e\n\u003cp\u003eThe photosensitizer on the surface of the donor beads is excited by irradiation with 680 nm laser light, converting surrounding oxygen into singlet oxygen. The singlet oxygen diffuses in the solution and, upon encountering an acceptor bead, triggers a cascade of amplified chemical reactions within it, ultimately resulting in light emission. As illustrated, the streptavidin on the donor bead binds to a biotinylated antibody. The antibody-labeled acceptor bead and the biotinylated antibody both bind to the IgG sample, generating the detection signal. This technology requires no washing steps, is simple to operate, and compared to traditional ELISA, offers a wider detection range, higher sensitivity, and lower background.\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/d9300a94-4c68-4b4e-ac17-382d4643c02f.png?access_token=c7b91711-f28e-4eae-97dc-6ff334638bed\" class=\"img img-fluid o_we_custom_image\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:none;\" border=\"1\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003cthead\u003e\u003ctr\u003e\n\u003cth style=\"border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e\u003cstrong\u003e200T\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e\u003cstrong\u003e500T\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e\u003cstrong\u003e5000T\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eStreptavidin-labeled Donor Beads\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e125×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e8 µL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e20 µL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e200 µL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eAnti-human IgG Antibody-labeled Acceptor Beads\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e500×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e2 µL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e5 µL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e50 µL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eBiotin-labeled Anti-human IgG Antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e625×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e2 µL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e5 µL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e50 µL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eIgG Standard\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e1 mg\/mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e5 µL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e10 µL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e20 µL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eAssay Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e1×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e4 mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e10 mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e100 mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\u003c\/figure\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cstrong style='-webkit-tap-highlight-color:transparent;-webkit-text-stroke-width:0px;background-color:rgb(255, 255, 255);box-sizing:border-box;color:rgb(0, 0, 0);font-family:\"Microsoft YaHei\";font-size:14px;font-style:normal;font-variant-caps:normal;font-variant-ligatures:normal;letter-spacing:normal;orphans:2;text-align:start;text-decoration-color:initial;text-decoration-style:initial;text-decoration-thickness:initial;text-indent:0px;text-transform:none;white-space:normal;widows:2;word-spacing:0px;'\u003eTaking a 384-well shallow well plate as an example\u003c\/strong\u003e\u003cbr\u003e\u003cspan style=\"background-color:rgb(255,255,255);color:rgb(0,0,0);\"\u003e\u003cspan style='-webkit-text-stroke-width:0px;display:inline !important;float:none;font-family:\"Microsoft YaHei\";font-size:14px;font-style:normal;font-variant-caps:normal;font-variant-ligatures:normal;font-weight:400;letter-spacing:normal;orphans:2;text-align:start;text-decoration-color:initial;text-decoration-style:initial;text-decoration-thickness:initial;text-indent:0px;text-transform:none;white-space:normal;widows:2;word-spacing:0px;'\u003eI. Preparation of Working Solutions\u003c\/span\u003e\u003c\/span\u003e\u003cbr\u003e\u003cspan style=\"background-color:rgb(255,255,255);color:rgb(0,0,0);\"\u003e\u003cspan style='-webkit-text-stroke-width:0px;display:inline !important;float:none;font-family:\"Microsoft YaHei\";font-size:14px;font-style:normal;font-variant-caps:normal;font-variant-ligatures:normal;font-weight:400;letter-spacing:normal;orphans:2;text-align:start;text-decoration-color:initial;text-decoration-style:initial;text-decoration-thickness:initial;text-indent:0px;text-transform:none;white-space:normal;widows:2;word-spacing:0px;'\u003eBefore using donor beads and acceptor beads, ensure the beads are in suspension. If there is slight clumping, resuspend the beads using sonication. Do not allow direct contact between beads and ice cubes. Protect from light during use.\u003c\/span\u003e\u003c\/span\u003e\u003cbr\u003e\u003cspan style=\"background-color:rgb(255,255,255);color:rgb(0,0,0);\"\u003e\u003cspan style='-webkit-text-stroke-width:0px;display:inline !important;float:none;font-family:\"Microsoft YaHei\";font-size:14px;font-style:normal;font-variant-caps:normal;font-variant-ligatures:normal;font-weight:400;letter-spacing:normal;orphans:2;text-align:start;text-decoration-color:initial;text-decoration-style:initial;text-decoration-thickness:initial;text-indent:0px;text-transform:none;white-space:normal;widows:2;word-spacing:0px;'\u003eAll components should be diluted with 1× Assay Diluent. It is recommended to store and dilute the standard on ice after thawing. The donor beads should be diluted according to the dilution factor into the pre-diluted biotinylated antibody. The volume of the mixed solution of the two reagents per well is 5uL.\u003c\/span\u003e\u003c\/span\u003e\u003cbr\u003e\u003cspan style=\"background-color:rgb(255,255,255);color:rgb(0,0,0);\"\u003e\u003cspan style='-webkit-text-stroke-width:0px;display:inline !important;float:none;font-family:\"Microsoft YaHei\";font-size:14px;font-style:normal;font-variant-caps:normal;font-variant-ligatures:normal;font-weight:400;letter-spacing:normal;orphans:2;text-align:start;text-decoration-color:initial;text-decoration-style:initial;text-decoration-thickness:initial;text-indent:0px;text-transform:none;white-space:normal;widows:2;word-spacing:0px;'\u003e1. Anti-human IgG antibody-labeled acceptor beads: After resuspending the acceptor beads, dilute them 500-fold with assay diluent. Use 5uL per well.\u003c\/span\u003e\u003c\/span\u003e\u003cbr\u003e\u003cspan style=\"background-color:rgb(255,255,255);color:rgb(0,0,0);\"\u003e\u003cspan style='-webkit-text-stroke-width:0px;display:inline !important;float:none;font-family:\"Microsoft YaHei\";font-size:14px;font-style:normal;font-variant-caps:normal;font-variant-ligatures:normal;font-weight:400;letter-spacing:normal;orphans:2;text-align:start;text-decoration-color:initial;text-decoration-style:initial;text-decoration-thickness:initial;text-indent:0px;text-transform:none;white-space:normal;widows:2;word-spacing:0px;'\u003e2. Biotin-labeled anti-human IgG antibody and streptavidin-labeled donor beads: After diluting the biotinylated antibody with assay diluent, directly dilute the donor beads into the diluted antibody. Use 5uL per well.\u003c\/span\u003e\u003c\/span\u003e\u003cbr\u003e\u003cspan style=\"background-color:rgb(255,255,255);color:rgb(0,0,0);\"\u003e\u003cspan style='-webkit-text-stroke-width:0px;display:inline !important;float:none;font-family:\"Microsoft YaHei\";font-size:14px;font-style:normal;font-variant-caps:normal;font-variant-ligatures:normal;font-weight:400;letter-spacing:normal;orphans:2;text-align:start;text-decoration-color:initial;text-decoration-style:initial;text-decoration-thickness:initial;text-indent:0px;text-transform:none;white-space:normal;widows:2;word-spacing:0px;'\u003e3. IgG Standard\/Test Samples: Store the standard on ice after thawing. Perform serial dilutions with assay diluent. Use 10uL per well.\u003c\/span\u003e\u003c\/span\u003e\u003cbr\u003e\u003cspan style=\"background-color:rgb(255,255,255);color:rgb(0,0,0);\"\u003e\u003cspan style='-webkit-text-stroke-width:0px;display:inline !important;float:none;font-family:\"Microsoft YaHei\";font-size:14px;font-style:normal;font-variant-caps:normal;font-variant-ligatures:normal;font-weight:400;letter-spacing:normal;orphans:2;text-align:start;text-decoration-color:initial;text-decoration-style:initial;text-decoration-thickness:initial;text-indent:0px;text-transform:none;white-space:normal;widows:2;word-spacing:0px;'\u003eAdjust dilutions according to the number of standard curves required for the experiment. For example: dilute 1uL of IgG standard into 249uL of assay diluent to obtain Std 1, then perform 3-fold serial dilutions up to Std 10.\u003c\/span\u003e\u003c\/span\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style='-webkit-tap-highlight-color:transparent;-webkit-text-stroke-width:0px;background-color:rgb(255, 255, 255);border-collapse:collapse;border-spacing:0px;box-sizing:border-box;break-inside:avoid;color:rgb(0, 0, 0);font-family:\"Microsoft YaHei\";font-size:14px;font-style:normal;font-variant-caps:normal;font-variant-ligatures:normal;font-weight:400;height:auto !important;letter-spacing:normal;margin:0px auto;max-width:100%;orphans:2;text-align:center;text-decoration-color:initial;text-decoration-style:initial;text-decoration-thickness:initial;text-transform:none;white-space:normal;widows:2;width:569.5px;word-spacing:0px;' border=\"1\"\u003e\u003ctbody style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;\"\u003e\n\u003ctr style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;\"\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;width:96.3646px;word-break:inherit;\" width=\"70\"\u003e\u003cstrong style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;\"\u003eStandard\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;width:360.552px;word-break:inherit;\" width=\"266\"\u003e\u003cstrong style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;\"\u003eStandard Dilution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;width:111.917px;word-break:inherit;\" width=\"81\"\u003e\u003cstrong style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;\"\u003eWorking Solution Concentration (ng\/mL)\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;\"\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;width:96.3646px;word-break:inherit;\" width=\"70\"\u003eStd 1\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;width:360.552px;word-break:inherit;\" width=\"266\"\u003e1uL Standard Stock + 249uL Standard Diluent\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;width:111.917px;word-break:inherit;\" width=\"81\"\u003e4000.00\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;\"\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;width:96.3646px;word-break:inherit;\" width=\"70\"\u003eStd 2\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;width:360.552px;word-break:inherit;\" width=\"266\"\u003e10uL Std 1 + 20uL Standard Diluent\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;width:111.917px;word-break:inherit;\" width=\"81\"\u003e1333.33\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;\"\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;width:96.3646px;word-break:inherit;\" width=\"70\"\u003eStd 3\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;width:360.552px;word-break:inherit;\" width=\"266\"\u003e10uL Std 2 + 20uL Standard Diluent\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;width:111.917px;word-break:inherit;\" width=\"81\"\u003e444.44\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;\"\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;width:96.3646px;word-break:inherit;\" width=\"70\"\u003eStd 4\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;width:360.552px;word-break:inherit;\" width=\"266\"\u003e10uL Std 3 + 20uL Standard Diluent\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;width:111.917px;word-break:inherit;\" width=\"81\"\u003e148.15\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;\"\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;width:96.3646px;word-break:inherit;\" width=\"70\"\u003eStd 5\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;width:360.552px;word-break:inherit;\" width=\"266\"\u003e10uL Std 4 + 20uL Standard Diluent\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;width:111.917px;word-break:inherit;\" width=\"81\"\u003e49.38\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;\"\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;width:96.3646px;word-break:inherit;\" width=\"70\"\u003eStd 6\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;width:360.552px;word-break:inherit;\" width=\"266\"\u003e10uL Std 5 + 20uL Standard Diluent\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;width:111.917px;word-break:inherit;\" width=\"81\"\u003e16.46\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;\"\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;width:96.3646px;word-break:inherit;\" width=\"70\"\u003eStd 7\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;width:360.552px;word-break:inherit;\" width=\"266\"\u003e10uL Std 6 + 20uL Standard Diluent\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;width:111.917px;word-break:inherit;\" width=\"81\"\u003e5.49\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;\"\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;width:96.3646px;word-break:inherit;\" width=\"70\"\u003eStd 8\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;width:360.552px;word-break:inherit;\" width=\"266\"\u003e10uL Std 7 + 20uL Standard Diluent\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;width:111.917px;word-break:inherit;\" width=\"81\"\u003e1.83\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;\"\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;width:96.3646px;word-break:inherit;\" width=\"70\"\u003eStd 9\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;width:360.552px;word-break:inherit;\" width=\"266\"\u003e10uL Std 8 + 20uL Standard Diluent\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;width:111.917px;word-break:inherit;\" width=\"81\"\u003e0.61\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;\"\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;width:96.3646px;word-break:inherit;\" width=\"70\"\u003eStd 10\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;width:360.552px;word-break:inherit;\" width=\"266\"\u003e10uL Std 9 + 20uL Standard Diluent\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;width:111.917px;word-break:inherit;\" width=\"81\"\u003e0.20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;\"\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;width:96.3646px;word-break:inherit;\" width=\"70\"\u003e0\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;width:360.552px;word-break:inherit;\" width=\"266\"\u003e20uL Standard Diluent\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;width:111.917px;word-break:inherit;\" width=\"81\"\u003e0.00\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cbr\u003e\u003cbr\u003e\u003cspan style=\"background-color:rgb(255,255,255);color:rgb(0,0,0);\"\u003e\u003cspan style='-webkit-text-stroke-width:0px;display:inline !important;float:none;font-family:\"Microsoft YaHei\";font-size:14px;font-style:normal;font-variant-caps:normal;font-variant-ligatures:normal;font-weight:400;letter-spacing:normal;orphans:2;text-align:start;text-decoration-color:initial;text-decoration-style:initial;text-decoration-thickness:initial;text-indent:0px;text-transform:none;white-space:normal;widows:2;word-spacing:0px;'\u003eII. Sample Addition\u003c\/span\u003e\u003c\/span\u003e\u003cbr\u003e\u003cspan style=\"background-color:rgb(255,255,255);color:rgb(0,0,0);\"\u003e\u003cspan style='-webkit-text-stroke-width:0px;display:inline !important;float:none;font-family:\"Microsoft YaHei\";font-size:14px;font-style:normal;font-variant-caps:normal;font-variant-ligatures:normal;font-weight:400;letter-spacing:normal;orphans:2;text-align:start;text-decoration-color:initial;text-decoration-style:initial;text-decoration-thickness:initial;text-indent:0px;text-transform:none;white-space:normal;widows:2;word-spacing:0px;'\u003eOnce samples, standards, and working solutions of detection reagents are prepared, add them and incubate according to the order specified in the table.\u003c\/span\u003e\u003c\/span\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style='-webkit-tap-highlight-color:transparent;-webkit-text-stroke-width:0px;background-color:rgb(255, 255, 255);border-collapse:collapse;border-spacing:0px;box-sizing:border-box;break-inside:avoid;color:rgb(0, 0, 0);font-family:\"Microsoft YaHei\";font-size:14px;font-style:normal;font-variant-caps:normal;font-variant-ligatures:normal;font-weight:400;height:auto !important;letter-spacing:normal;margin-left:auto;margin-right:auto;max-width:100%;orphans:2;text-align:center;text-decoration-color:initial;text-decoration-style:initial;text-decoration-thickness:initial;text-transform:none;white-space:normal;widows:2;width:761.469px;word-spacing:0px;' border=\"1\"\u003e\u003ctbody style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;\"\u003e\n\u003ctr style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;\"\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;text-align:center;width:212.021px;word-break:inherit;\" rowspan=\"2\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;text-align:center;width:130.958px;word-break:inherit;\" rowspan=\"2\"\u003eTest Well\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;text-align:center;width:417.823px;word-break:inherit;\" colspan=\"3\"\u003eControl Wells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;\"\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;text-align:center;width:136.906px;word-break:inherit;\"\u003eNegative Control\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;text-align:center;width:142.76px;word-break:inherit;\"\u003eDonor Bead Control\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;text-align:center;width:138.156px;word-break:inherit;\"\u003eAcceptor Bead Control\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;\"\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;text-align:center;width:212.021px;word-break:inherit;\"\u003eIgG Standard\/Test Sample\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;text-align:center;width:130.958px;word-break:inherit;\"\u003e10uL\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;text-align:center;width:136.906px;word-break:inherit;\"\u003e--\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;text-align:center;width:142.76px;word-break:inherit;\"\u003e--\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;text-align:center;width:138.156px;word-break:inherit;\"\u003e--\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;\"\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;text-align:center;width:212.021px;word-break:inherit;\"\u003eDiluent\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;text-align:center;width:130.958px;word-break:inherit;\"\u003e--\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;text-align:center;width:136.906px;word-break:inherit;\"\u003e10uL\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;text-align:center;width:142.76px;word-break:inherit;\"\u003e15uL\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;text-align:center;width:138.156px;word-break:inherit;\"\u003e10uL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;\"\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;text-align:center;width:212.021px;word-break:inherit;\"\u003eAnti-human IgG antibody-labeled acceptor beads\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;text-align:center;width:130.958px;word-break:inherit;\"\u003e5uL\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;text-align:center;width:136.906px;word-break:inherit;\"\u003e5uL\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;text-align:center;width:142.76px;word-break:inherit;\"\u003e--\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;text-align:center;width:138.156px;word-break:inherit;\"\u003e5uL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;\"\u003e\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;width:760.802px;word-break:inherit;\" colspan=\"5\"\u003eStep 1: Incubate at room temperature for 1 hour\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;\"\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;text-align:center;width:212.021px;word-break:inherit;\"\u003eBiotin-labeled anti-human IgG antibody and streptavidin-labeled donor beads\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;text-align:center;width:130.958px;word-break:inherit;\"\u003e 5uL\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;text-align:center;width:136.906px;word-break:inherit;\"\u003e 5uL\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;text-align:center;width:142.76px;word-break:inherit;\"\u003e 5uL\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;text-align:center;width:138.156px;word-break:inherit;\"\u003e--\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;\"\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;text-align:center;width:212.021px;word-break:inherit;\"\u003eDiluent\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;text-align:center;width:130.958px;word-break:inherit;\"\u003e--\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;text-align:center;width:136.906px;word-break:inherit;\"\u003e--\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;text-align:center;width:142.76px;word-break:inherit;\"\u003e--\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;text-align:center;width:138.156px;word-break:inherit;\"\u003e5uL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;\"\u003e\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;padding:0px;width:760.802px;word-break:inherit;\" colspan=\"5\"\u003eStep 2: Incubate at room temperature for 1 hour\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cbr\u003e\u003cspan style=\"background-color:rgb(255,255,255);color:rgb(0,0,0);\"\u003e\u003cspan style='-webkit-text-stroke-width:0px;display:inline !important;float:none;font-family:\"Microsoft YaHei\";font-size:14px;font-style:normal;font-variant-caps:normal;font-variant-ligatures:normal;font-weight:400;letter-spacing:normal;orphans:2;text-align:start;text-decoration-color:initial;text-decoration-style:initial;text-decoration-thickness:initial;text-indent:0px;text-transform:none;white-space:normal;widows:2;word-spacing:0px;'\u003eThe experiment should include the following control wells:\u003c\/span\u003e\u003c\/span\u003e\u003cbr\u003e\u003cspan style=\"background-color:rgb(255,255,255);color:rgb(0,0,0);\"\u003e\u003cspan style='-webkit-text-stroke-width:0px;display:inline !important;float:none;font-family:\"Microsoft YaHei\";font-size:14px;font-style:normal;font-variant-caps:normal;font-variant-ligatures:normal;font-weight:400;letter-spacing:normal;orphans:2;text-align:start;text-decoration-color:initial;text-decoration-style:initial;text-decoration-thickness:initial;text-indent:0px;text-transform:none;white-space:normal;widows:2;word-spacing:0px;'\u003e\u003cbr\u003e(1) Buffer Control (Negative Control): To exclude the effect of the buffer on signal values, serving as a background value for calculating the specific signal of the reaction system.\u003c\/span\u003e\u003c\/span\u003e\u003cbr\u003e\u003cspan style=\"background-color:rgb(255,255,255);color:rgb(0,0,0);\"\u003e\u003cspan style='-webkit-text-stroke-width:0px;display:inline !important;float:none;font-family:\"Microsoft YaHei\";font-size:14px;font-style:normal;font-variant-caps:normal;font-variant-ligatures:normal;font-weight:400;letter-spacing:normal;orphans:2;text-align:start;text-decoration-color:initial;text-decoration-style:initial;text-decoration-thickness:initial;text-indent:0px;text-transform:none;white-space:normal;widows:2;word-spacing:0px;'\u003e\u003cbr\u003e(2) Donor\/Acceptor Bead Control Wells: To detect the signal value of isolated donor\/acceptor beads in the buffer, used to verify the normal signal value and background of the beads.\u003c\/span\u003e\u003c\/span\u003e\u003cbr\u003e\u003cbr\u003e\u003cspan style=\"background-color:rgb(255,255,255);color:rgb(0,0,0);\"\u003e\u003cspan style='-webkit-text-stroke-width:0px;display:inline !important;float:none;font-family:\"Microsoft YaHei\";font-size:14px;font-style:normal;font-variant-caps:normal;font-variant-ligatures:normal;font-weight:400;letter-spacing:normal;orphans:2;text-align:start;text-decoration-color:initial;text-decoration-style:initial;text-decoration-thickness:initial;text-indent:0px;text-transform:none;white-space:normal;widows:2;word-spacing:0px;'\u003e\u003cbr\u003eIII. Detection\u003c\/span\u003e\u003c\/span\u003e\u003cbr\u003e\u003cspan style=\"background-color:rgb(255,255,255);color:rgb(0,0,0);\"\u003e\u003cspan style='-webkit-text-stroke-width:0px;display:inline !important;float:none;font-family:\"Microsoft YaHei\";font-size:14px;font-style:normal;font-variant-caps:normal;font-variant-ligatures:normal;font-weight:400;letter-spacing:normal;orphans:2;text-align:start;text-decoration-color:initial;text-decoration-style:initial;text-decoration-thickness:initial;text-indent:0px;text-transform:none;white-space:normal;widows:2;word-spacing:0px;'\u003e\u003cbr\u003eBefore the experiment, please confirm the functions and configuration of the microplate reader. Perform detection on a compatible homogeneous chemiluminescence microplate reader.\u003c\/span\u003e\u003c\/span\u003e\u003cbr\u003e\u003cbr\u003e\u003cspan style=\"background-color:rgb(255,255,255);color:rgb(0,0,0);\"\u003e\u003cspan style='-webkit-text-stroke-width:0px;display:inline !important;float:none;font-family:\"Microsoft YaHei\";font-size:14px;font-style:normal;font-variant-caps:normal;font-variant-ligatures:normal;font-weight:400;letter-spacing:normal;orphans:2;text-align:start;text-decoration-color:initial;text-decoration-style:initial;text-decoration-thickness:initial;text-indent:0px;text-transform:none;white-space:normal;widows:2;word-spacing:0px;'\u003e\u003cbr\u003eIV. Result Calculation\u003c\/span\u003e\u003c\/span\u003e\u003cbr\u003e\u003cspan style=\"background-color:rgb(255,255,255);color:rgb(0,0,0);\"\u003e\u003cspan style='-webkit-text-stroke-width:0px;display:inline !important;float:none;font-family:\"Microsoft YaHei\";font-size:14px;font-style:normal;font-variant-caps:normal;font-variant-ligatures:normal;font-weight:400;letter-spacing:normal;orphans:2;text-align:start;text-decoration-color:initial;text-decoration-style:initial;text-decoration-thickness:initial;text-indent:0px;text-transform:none;white-space:normal;widows:2;word-spacing:0px;'\u003e\u003cbr\u003e1. Calculate the net signal ratio of acceptor and donor emission signals for each well\u003c\/span\u003e\u003c\/span\u003e\u003cbr\u003e\u003cspan style=\"background-color:rgb(255,255,255);color:rgb(0,0,0);\"\u003e\u003cspan style='-webkit-text-stroke-width:0px;display:inline !important;float:none;font-family:\"Microsoft YaHei\";font-size:14px;font-style:normal;font-variant-caps:normal;font-variant-ligatures:normal;font-weight:400;letter-spacing:normal;orphans:2;text-align:start;text-decoration-color:initial;text-decoration-style:initial;text-decoration-thickness:initial;text-indent:0px;text-transform:none;white-space:normal;widows:2;word-spacing:0px;'\u003e\u003cbr\u003eNet signal = [signal (Test Well) - signal (Negative Control Well)] \/ signal (Negative Control Well)*100\u003c\/span\u003e\u003c\/span\u003e\u003cbr\u003e\u003cspan style=\"background-color:rgb(255,255,255);color:rgb(0,0,0);\"\u003e\u003cspan style='-webkit-text-stroke-width:0px;display:inline !important;float:none;font-family:\"Microsoft YaHei\";font-size:14px;font-style:normal;font-variant-caps:normal;font-variant-ligatures:normal;font-weight:400;letter-spacing:normal;orphans:2;text-align:start;text-decoration-color:initial;text-decoration-style:initial;text-decoration-thickness:initial;text-indent:0px;text-transform:none;white-space:normal;widows:2;word-spacing:0px;'\u003e\u003cbr\u003e2. Calculate the coefficient of variation (CV%) for replicate wells\u003c\/span\u003e\u003c\/span\u003e\u003cbr\u003e\u003cspan style=\"background-color:rgb(255,255,255);color:rgb(0,0,0);\"\u003e\u003cspan style='-webkit-text-stroke-width:0px;display:inline !important;float:none;font-family:\"Microsoft YaHei\";font-size:14px;font-style:normal;font-variant-caps:normal;font-variant-ligatures:normal;font-weight:400;letter-spacing:normal;orphans:2;text-align:start;text-decoration-color:initial;text-decoration-style:initial;text-decoration-thickness:initial;text-indent:0px;text-transform:none;white-space:normal;widows:2;word-spacing:0px;'\u003e\u003cbr\u003eCoefficient of Variation CV% = Standard Deviation\/Mean*100%\u003c\/span\u003e\u003c\/span\u003e\u003cbr\u003e\u003cbr\u003e\u003cspan style=\"background-color:rgb(255,255,255);color:rgb(0,0,0);\"\u003e\u003cspan style='-webkit-text-stroke-width:0px;display:inline !important;float:none;font-family:\"Microsoft YaHei\";font-size:14px;font-style:normal;font-variant-caps:normal;font-variant-ligatures:normal;font-weight:400;letter-spacing:normal;orphans:2;text-align:start;text-decoration-color:initial;text-decoration-style:initial;text-decoration-thickness:initial;text-indent:0px;text-transform:none;white-space:normal;widows:2;word-spacing:0px;'\u003e\u003cbr\u003eV. Example Data\u003c\/span\u003e\u003c\/span\u003e\u003cbr\u003e\u003cspan style=\"background-color:rgb(255,255,255);color:rgb(0,0,0);\"\u003e\u003cspan style='-webkit-text-stroke-width:0px;display:inline !important;float:none;font-family:\"Microsoft YaHei\";font-size:14px;font-style:normal;font-variant-caps:normal;font-variant-ligatures:normal;font-weight:400;letter-spacing:normal;orphans:2;text-align:start;text-decoration-color:initial;text-decoration-style:initial;text-decoration-thickness:initial;text-indent:0px;text-transform:none;white-space:normal;widows:2;word-spacing:0px;'\u003eThe following examples are for reference only. Specific experimental systems should be adjusted and optimized according to actual conditions. For each detection, sample concentrations should be calculated using the standard curve generated from the standards run in the same experiment.\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"200T","offer_id":42302162665547,"sku":null,"price":959.0,"currency_code":"USD","in_stock":true},{"title":"500T","offer_id":42302162698315,"sku":null,"price":1599.0,"currency_code":"USD","in_stock":true},{"title":"5000T","offer_id":42302162731083,"sku":null,"price":13241.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/26f8e15d-7a31-4679-83da-e06a57eefe44.jpg?v=1780633682","url":"https:\/\/www.antbioinc.com\/products\/human-igg-kit-hica-ua086034","provider":"AntBio","version":"1.0","type":"link"}