{"product_id":"hpsc-induced-differentiation-small-intestine-organoid-kit-abs90485","title":"hPSC诱导分化小肠类器官试剂盒","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e I. Experimental Instruments and Materials \u003cbr\u003e\u003c\/strong\u003e Instruments: Biosafety cabinet, cell incubator, horizontal centrifuge, inverted microscope, cryogenic refrigerator \u003cbr\u003e Materials: Cell culture plates (specifications are: 6 hole, 12 hole, 24 Hole), centrifuge tube (specifications are 15 mL  and  50 mL ), pipette (specifications are  10 μL 、 100 μL 、 1000 μL ), sterile tip (specifications are  10 μL 、 200 μL  and  1000 μL ), pipette (specifications are 10mL 、 50mL ） \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e II. Experimental Methods \u003cbr\u003e1 Preparation of matrix glue \u003c\/strong\u003e\u003cbr\u003e\u003cdiv style=\"text-align: center;\"\u003e （ 1 ) Laying the slabs hPSC When using cells, a stem cell matrix gel should be used. The matrix gel should be dispensed according to the operating instructions for each batch of the product. Subsequent dispensing of the matrix gel... 4℃ Thawing below, with containing 15mM HEPEs of DMEM\/F12 Mix evenly, lay on a board, and set aside. \u003cbr\u003e Table 1.  Recommended substrate volume for culture vessel coating \u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 100%;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 48.7953%;\"\u003e Culture vessel for tissue culture treatment \u003c\/td\u003e\n\u003ctd style=\"width: 48.7953%;\"\u003e Volume of Diluted Matrix \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 48.7953%;\" align=\"center\" valign=\"middle\" width=\"349\"\u003e\u003cp\u003e24 orifice plate \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 48.7953%;\" align=\"center\" valign=\"middle\" width=\"220\"\u003e\u003cp\u003e250 µL\/ hole \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 48.7953%;\" align=\"center\" valign=\"middle\" width=\"349\"\u003e\u003cp\u003e12 orifice plate \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 48.7953%;\" align=\"center\" valign=\"middle\" width=\"220\"\u003e\u003cp\u003e500 µL\/ hole \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 48.7953%;\" align=\"center\" valign=\"middle\" width=\"349\"\u003e\u003cp\u003e6 orifice plate \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 48.7953%;\" align=\"center\" valign=\"middle\" width=\"220\"\u003e\u003cp\u003e1 mL\/ hole \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 48.7953%;\" align=\"center\" valign=\"middle\" width=\"349\"\u003e\u003cp\u003e100 mm  Petri dish \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 48.7953%;\" align=\"center\" valign=\"middle\" width=\"220\"\u003e\u003cp\u003e6 mL\/ dish \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 48.7953%;\" align=\"center\" valign=\"middle\" width=\"349\"\u003e\u003cp\u003eT-25 cm²  culture bottle \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 48.7953%;\" align=\"center\" valign=\"middle\" width=\"220\"\u003e\u003cp\u003e3 mL\/ bottle \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 48.7953%;\" align=\"center\" valign=\"middle\" width=\"349\"\u003e\u003cp\u003eT-75 cm²  culture bottle \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 48.7953%;\" align=\"center\" valign=\"middle\" width=\"220\"\u003e\u003cp\u003e8 mL\/ bottle \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cp\u003e\u003cstrong\u003e2 , endoderm ( DE Preparation of culture medium \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e （ 1 ) in the  0  God, prepare the first  0 、 1  and  2  Daily requirements  DE  Culture medium (basic medium 1 +  supplements A ) volume ( 1.7 mL\/ hole). \u003c\/p\u003e\n\u003cp\u003e （ 2 ) to prepare  2 mL DE  Taking the culture medium as an example: \u003c\/p\u003e\n\u003cp\u003e Thaw replenishment on ice A 。 Mix thoroughly. \u003c\/p\u003e\n\u003cp\u003e Note: If not used immediately, repackage and store in  -20°C  Lower storage. Do not exceed the shelf life of the supplement. After thawing aliquots, use immediately, do not refreeze. \u003c\/p\u003e\n\u003cp\u003e At room temperature  (15 - 25°C)  thaw 1h or   In 4°C Thaw the entire bottle of basic culture medium overnight in the refrigerator. 1 Mix thoroughly. \u003c\/p\u003e\n\u003cp\u003e Note: If not used immediately, it can be used in  2 - 8°C  Store for up to  2  months. Alternatively, repackage and  -20°C  Lower storage. Do not exceed the expiration date indicated on the label. After thawing the aliquot, use immediately or in  2 - 8°C  Store for up to  2  Zhou, do not refreeze. \u003c\/p\u003e\n\u003cp\u003e will  20 μL  supplements A join  1.98 mL  cold  (2 - 8°C) Basic medium 1 middle. After thorough mixing, store in  2 - 8°C 。 \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e3 Differentiation into endoderm cells ( DE )(with 24 (Taking orifice plates as an example) \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e （ 1 )th  0  Day: Preheating  (37°C)  No  0  Daily requirements  DE  medium volume  (0.7 mL\/ hole ) 。 The remaining  DE  The culture medium is stored in  2 - 8°C 。 \u003c\/p\u003e\n\u003cp\u003e From  hPSC The cells are completely absorbed and cultured. Add dropwise to each well of the tilted culture plate along the well wall.  0.7 mL DE  culture medium. In  37°C 、 5% CO₂  and  95%  Most incubated under humidity  24  Hours. \u003c\/p\u003e\n\u003cp\u003e （ 2 )th  1  Day: Preheating  (37°C)  No  1  Required for daily use  DE  medium volume  (0.5 mL\/ hole ) 。 The remaining  DE  The culture medium is stored in  2 - 8°C 。 The culture medium is aspirated from the cells. Add dropwise to each well of the tilted culture plate along the well wall.  0.5 mL DE  culture medium. In  37°C 、 5% CO₂  and  95%  Incubation under humidity  24  Hours. \u003c\/p\u003e\n\u003cp\u003e （ 3 )th  2  Day: Preheating (37°C)  The remaining  DE  culture medium. The culture medium is aspirated from the cells. Add dropwise to each well of the tilted culture plate along the well wall.  0.5 mL DE  culture medium. In  37°C 、 5% CO₂  and  95%  Incubation under humidity  24  Hours. \u003c\/p\u003e\n\u003cp\u003e （ 4 )th  3  Day: Cells can detect the formation of definitive endoderm. Multiporous cells can continue to differentiate into middle and hindgut spheres ( MH ）。 \u003c\/p\u003e\n\u003cp\u003e Note: During definitive endoderm induction, cells appear to undergo massive cell death. Shorten the cell life as much as possible  37°C  Time outside the incubator. pass  24  After hours of definitive endoderm induction, the cells are very sensitive and require careful medium changes. incubation  72  After hours, a confluent monolayer of closely aligned endoderm cells will form. \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e4 ,middle \/ hindgut  (MH) Preparation of culture medium \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e （ 1 ) in the  3  God, prepare the first  3 - 8  Daily requirements  MH  Culture medium (basic medium 1 + supplements  B + supplements  C ) volume ( 3 mL\/ hole). \u003c\/p\u003e\n\u003cp\u003e （ 2 ) to prepare  3 mL MH  Taking the culture medium as an example: \u003c\/p\u003e\n\u003cp\u003e Thaw replenishment on ice  B  and  C 。 After mixing in basic culture medium 1 Previously kept on ice. Mix thoroughly. \u003c\/p\u003e\n\u003cp\u003e Note: If not used immediately, repackage and store in  -20°C  Lower storage. Do not exceed the shelf life of the supplement. After thawing aliquots, use immediately, do not refreeze. will  30 µL  supplements  B and  30 µL  supplements  C  join  2.94 mL  cold  (2 - 8°C) Basic medium 1 middle. Mix well and store in  2 - 8°C 。 \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e5 Endoderm cells differentiate into middle \/ hindgut  (MH)\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e （ 1 )th  3  Day: Preheating (15 - 25°C)  sufficient  MH  culture medium  (0.5 mL\/ hole ) 。 The remaining  MH  The culture medium is stored in  2 - 8°C 。 \u003c\/p\u003e\n\u003cp\u003e （ 2 Remove the culture medium from the endoderm cells and replace it with...  0.5 mL MH  culture medium. In  37°C 、 5% CO2 and  95%  Incubation under humidity  24  Hours. \u003c\/p\u003e\n\u003cp\u003e （ 3 )th  4 - 9  Day: As described below, each  24  Complete media changes were performed every hour and spheroids were evaluated. \u003c\/p\u003e\n\u003cp\u003e Note: Ensure that the culture is removed.  30  Return to the incubator within minutes. \u003c\/p\u003e\n\u003cp\u003ea.  The monolayers were observed under a microscope. As early as the differentiation stage  4  It may be observed in the sky.  3D  Structure. The free-floating middle and hindgut spheroids will be in the first stage of differentiation.  5 - 9  The sky appeared. \u003c\/p\u003e\n\u003cp\u003eb.  Use  1 mL  Pipette, suction from the cells  0.5 mL  Culture medium, transferred to sterile  24  The wells were in clear flat-bottomed plates to assess the number and concentration of middle and posterior intestinal spheroids released from the cell monolayer. \u003c\/p\u003e\n\u003cp\u003ec.  Add to cells  0.5 mL  fresh  MH  culture medium. In  37°C 、 5% CO2 and  95%  Incubate under humidity. \u003c\/p\u003e\n\u003cp\u003e Note: Although  5 - 9  All spheroids released on the day can produce small intestinal organoids, but the length of time the cells are exposed to the midgut culture medium will control the regional characteristics of developing small intestinal organoids, such as the duodenum (shorter exposure time) or the ileum (longer exposure time). The timing of peak midgut spheroid production may vary.  hPSC  It varies by cell line. To obtain reproducible experimental results, midgut spheroids generated on the same day of differentiation should always be used to harvest and initiate human intestinal organoid cultures. Usually at the stage of differentiation  8  The largest number of sprouts was observed on that day. \u003c\/p\u003e\n\u003cp\u003e （ 4 On the day of spheroid embedding: Using a pipette, add the spheroid suspension from each well to a new one.  24  Counting was performed in one well of the well plate. Approximately  50  Corresponding volume addition of free floating spheres (based on previous sphere count)  15 mL  In a conical tube. Culture of intestinal organoids was performed. \u003c\/p\u003e\n\u003cp\u003e Note: The middle and posterior intestinal spheroid is the diameter  ≥ 75 µm  The cell aggregates have the potential to produce a human intestinal organoid. Multiple fused spheroids should be counted as a unit that will generate a human intestinal organoid. The remaining monolayer cultures can be used to detect the formation of the midgut or further differentiate in subsequent days to embed more spheroids. \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e6 Human intestinal organoid growth medium  (OGM)  Preparation of \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e （ 1 Prepare the necessary intestines OGM Culture medium (basic medium 2+ supplements D ) volume ( 2 mL\/ hole). \u003c\/p\u003e\n\u003cp\u003e （ 2 ) to prepare  2 mL intestinal tract  OGM  Taking the culture medium as an example: \u003c\/p\u003e\n\u003cp\u003e Thaw replenishment on ice D 。 After mixing in basic culture medium 2 Previously kept on ice. Mix thoroughly. \u003c\/p\u003e\n\u003cp\u003e Note: If not used immediately, repackage and store in  -20°C  Lower storage. Do not exceed the shelf life of the supplement. After thawing aliquots, use immediately, do not refreeze. \u003c\/p\u003e\n\u003cp\u003e will  40 µL  intestinal organoid supplement D join  1.96 mL  cold  (2 - 8°C)  Basic medium 2 middle. Mix thoroughly. Preheat to room temperature before use  (15 - 25°C) 。 In  2 - 8°C  Store for up to  2  Zhou. \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e7 Embedding the spheroid in  Matrigel middle \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e （ 1 The low growth factor matrix gel was subpackaged and frozen for storage (each aliquot of reagent). 200μL  For 4 (a sample). \u003c\/p\u003e\n\u003cp\u003e （ 2 Thaw an aliquot of low growth factor matrix gel on ice (each sample requires...).  50 µL  Low growth factor matrix glue). A box of sterile  100 µL  The pipette tip is placed in  -20°C 。 \u003c\/p\u003e\n\u003cp\u003e （ 3 ) and other collected MH The spheroids settled to the bottom of the conical tube, and the supernatant was carefully aspirated and discarded. \u003c\/p\u003e\n\u003cp\u003e （ 4 Add to the spheroid 1 mL Contains 15 mM HEPES  of DMEM\/F-12 。 At room temperature  (15 - 25°C)  Below  300xg  centrifuge  5  minutes. \u003c\/p\u003e\n\u003cp\u003e （ 5 ) Use  1 mL  Pipette, carefully aspirate and discard the supernatant. \u003c\/p\u003e\n\u003cp\u003e Note: Remove the supernatant as much as possible. \u003c\/p\u003e\n\u003cp\u003e （ 6 The thawed matrix gel and cell suspension were placed on an ice box and aspirated using a precooled gun tip. 50μL Mix the matrix gel with the cell suspension, taking care to avoid bubbles. \u003c\/p\u003e\n\u003cp\u003e （ 7 Using a pre-cooled gun tip, press each hole. 50μL Seed the cell-containing matrix gel into 24 On the well plate, when the mixture is slowly injected, the gun tip is gradually moved upwards so that the cells are evenly distributed in the glue. \u003c\/p\u003e\n\u003cp\u003e （ 8 Place the culture plate in an incubator. 20-30min Solidifying the gel droplets; \u003c\/p\u003e\n\u003cp\u003e （ 9 Carefully add along the hole wall. 500μL Restoring the intestines to room temperature (OGM) Organoid medium, every other 3-4 Change the liquid every day. \u003c\/p\u003e\n\u003cp\u003e （ 10 ) to 7-10 Once the intestinal organoids mature, passage, identification, and related experiments can be carried out. \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e This product is hPSC Induced differentiation intestinal organoid kit, human pluripotent stem cells hPSC Induction of differentiation by this kit results in intestinal organoids composed of cells of the intestinal tract, which, after maturation, consist of polarized intestinal epithelium (patterned into villous structures) and surrounding mesenchymal cells that produce microenvironmental factors. \u003cbr\u003e This product requires operators to have hPSC Experience in cell culture and a certain understanding of organoids. \u003cbr\u003e\u003cstrong\u003e\u003cstrong\u003e Product Composition: \u003c\/strong\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 387px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.8882%; height: 22px;\"\u003e\u003cstrong\u003e Research Phase \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.8882%; height: 22px;\"\u003e\u003cstrong\u003e Product Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.8882%; height: 22px;\"\u003e\u003cstrong\u003e Product Specifications \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.8882%; height: 22px;\"\u003e\u003cstrong\u003e store \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 108px; width: 23.8882%;\" rowspan=\"2\"\u003e definitive endoderm (DE) Stage \u003c\/td\u003e\n\u003ctd style=\"width: 23.8882%; height: 54px;\" align=\"center\" valign=\"middle\" width=\"157\"\u003e\u003cp\u003e Basic medium 1\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.8882%; height: 54px;\" align=\"center\" valign=\"middle\" width=\"99\"\u003e\u003cp\u003e100mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.8882%; height: 54px;\" align=\"center\" valign=\"middle\" width=\"134\"\u003e\u003cp\u003e-20℃ ， 12 Month \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 54px;\"\u003e\n\u003ctd style=\"width: 23.8882%; height: 54px;\" align=\"center\" valign=\"middle\" width=\"157\"\u003e\u003cp\u003e supplements A\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.8882%; height: 54px;\" align=\"center\" valign=\"middle\" width=\"99\"\u003e\u003cp\u003e1100μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.8882%; height: 54px;\" align=\"center\" valign=\"middle\" width=\"134\"\u003e\u003cp\u003e-20℃ ， 6 Month \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.8882%; height: 148px;\" rowspan=\"3\"\u003e Middle and posterior intestinal spheres ( MH Differentiation stage \u003c\/td\u003e\n\u003ctd style=\"width: 23.8882%; height: 40px;\" align=\"center\" valign=\"middle\" width=\"157\"\u003e\u003cp\u003e Basic medium 1\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.8882%; height: 40px;\" align=\"center\" valign=\"middle\" width=\"99\"\u003e\u003cp\u003e100mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.8882%; height: 40px;\" align=\"center\" valign=\"middle\" width=\"134\"\u003e\u003cp\u003e-20℃ ， 12 Month \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 54px;\"\u003e\n\u003ctd style=\"width: 23.8882%; height: 54px;\" align=\"center\" valign=\"middle\" width=\"157\"\u003e\u003cp\u003e supplements B\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.8882%; height: 54px;\" align=\"center\" valign=\"middle\" width=\"99\"\u003e\u003cp\u003e640μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.8882%; height: 54px;\" align=\"center\" valign=\"middle\" width=\"134\"\u003e\u003cp\u003e-20℃ ， 6 Month \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 54px;\"\u003e\n\u003ctd style=\"width: 23.8882%; height: 54px;\" align=\"center\" valign=\"middle\" width=\"157\"\u003e\u003cp\u003e supplements C\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.8882%; height: 54px;\" align=\"center\" valign=\"middle\" width=\"99\"\u003e\u003cp\u003e640μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.8882%; height: 54px;\" align=\"center\" valign=\"middle\" width=\"134\"\u003e\u003cp\u003e-20℃ ， 6 Month \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.8882%; height: 109px;\" rowspan=\"2\"\u003e intestines ( 3D ) Organoid stage \u003c\/td\u003e\n\u003ctd style=\"width: 23.8882%; height: 53px;\" align=\"center\" valign=\"middle\" width=\"157\"\u003e Basic medium 2\u003c\/td\u003e\n\u003ctd style=\"width: 23.8882%; height: 53px;\" align=\"center\" valign=\"middle\" width=\"99\"\u003e100mL\u003c\/td\u003e\n\u003ctd style=\"width: 23.8882%; height: 53px;\" align=\"center\" valign=\"middle\" width=\"169\"\u003e-20℃ ， 12 Month \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 56px;\"\u003e\n\u003ctd style=\"width: 23.8882%; height: 56px;\" align=\"center\" valign=\"middle\" width=\"157\"\u003e supplements D\u003c\/td\u003e\n\u003ctd style=\"width: 23.8882%; height: 56px;\" align=\"center\" valign=\"middle\" width=\"99\"\u003e2mL\u003c\/td\u003e\n\u003ctd style=\"width: 23.8882%; height: 56px;\" align=\"center\" valign=\"middle\" width=\"169\"\u003e-20℃ ， 6 Month \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch3\u003e \u003c\/h3\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ePlease refer to the product composition for details.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1Kit","offer_id":43224461181003,"sku":"abs90485-1Kit","price":3175.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.antbioinc.com\/products\/hpsc-induced-differentiation-small-intestine-organoid-kit-abs90485","provider":"AntBio","version":"1.0","type":"link"}