{"product_id":"hpsc-derived-colon-organoid-kit-abs90484","title":"hPSC诱导分化结肠类器官试剂盒","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e I. Experimental Instruments and Materials \u003cbr\u003e\u003c\/strong\u003e Instruments: Biosafety cabinet, cell incubator, horizontal centrifuge, inverted microscope, cryogenic refrigerator \u003cbr\u003e Materials: Cell culture plates (specifications are: 6 hole, 12 hole, 24 hole, 96 Hole), centrifuge tube (specifications are 15 mL  and  50 mL ), pipette (specifications are  10 μL 、 100 μL 、 1000 μL ), sterile tip (specifications are  10 μL 、 200 μL  and  1000 μL ), pipette (specifications are 10mL 、 50mL ） \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e II. Experimental Methods \u003cbr\u003e1 Preparation of matrix glue \u003c\/strong\u003e\u003cbr\u003e （ 1 ) Laying the slabs hPSC When using cells, a stem cell matrix gel should be used. The matrix gel should be dispensed according to the operating instructions for each batch of the product. Subsequent dispensing of the matrix gel... 4℃ Thawing below, with containing 15mM HEPEs of DMEM\/F12 Mix evenly, lay on a board, and set aside. \u003cdiv style=\"text-align: center;\"\u003e Table 1.  Recommended substrate volume for culture vessel coating \u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 100%;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 48.7953%;\"\u003e Culture vessel for tissue culture treatment \u003c\/td\u003e\n\u003ctd style=\"width: 48.7953%;\"\u003e Volume of Diluted Matrix \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 48.7953%;\" align=\"center\" valign=\"middle\" width=\"349\"\u003e\u003cp\u003e24 orifice plate \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 48.7953%;\" align=\"center\" valign=\"middle\" width=\"220\"\u003e\u003cp\u003e250 µL\/ hole \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 48.7953%;\" align=\"center\" valign=\"middle\" width=\"349\"\u003e\u003cp\u003e12 orifice plate \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 48.7953%;\" align=\"center\" valign=\"middle\" width=\"220\"\u003e\u003cp\u003e500 µL\/ hole \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 48.7953%;\" align=\"center\" valign=\"middle\" width=\"349\"\u003e\u003cp\u003e6 orifice plate \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 48.7953%;\" align=\"center\" valign=\"middle\" width=\"220\"\u003e\u003cp\u003e1 mL\/ hole \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 48.7953%;\" align=\"center\" valign=\"middle\" width=\"349\"\u003e\u003cp\u003e100 mm  Petri dish \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 48.7953%;\" align=\"center\" valign=\"middle\" width=\"220\"\u003e\u003cp\u003e6 mL\/ dish \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 48.7953%;\" align=\"center\" valign=\"middle\" width=\"349\"\u003e\u003cp\u003eT-25 cm²  culture bottle \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 48.7953%;\" align=\"center\" valign=\"middle\" width=\"220\"\u003e\u003cp\u003e3 mL\/ bottle \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 48.7953%;\" align=\"center\" valign=\"middle\" width=\"349\"\u003e\u003cp\u003eT-75 cm²  culture bottle \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 48.7953%;\" align=\"center\" valign=\"middle\" width=\"220\"\u003e\u003cp\u003e8 mL\/ bottle \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cstrong\u003e2 , endoderm ( DE Preparation of culture medium \u003c\/strong\u003e\u003cbr\u003e\u003cp\u003e Note: Kit can be completed 24 orifice plate 20 One hole hPSC From DE For the first differentiation of the hindgut, it is recommended to use... 2-3 Try each hole. \u003c\/p\u003e\n\u003cp\u003e （ 1 ) in the  0  God, prepare the first  0 、 1  and  2  Daily requirements  DE  Culture medium (basic medium 1 +  supplements A ) volume ( 1.7 mL\/ hole). \u003c\/p\u003e\n\u003cp\u003e （ 2 ) to prepare  2 mL DE  Take the culture medium as an example: thaw the supplement on ice A 。 Mix thoroughly. \u003c\/p\u003e\n\u003cp\u003e Note: If not used immediately, repackage and store in  -20°C  Lower storage. Do not exceed the shelf life of the supplement. After thawing aliquots, use immediately, do not refreeze. \u003c\/p\u003e\n\u003cp\u003e At room temperature  (15 - 25°C)  thaw 1h or   In 4°C Thaw the entire bottle of basic culture medium overnight in the refrigerator. 1 Mix thoroughly. \u003c\/p\u003e\n\u003cp\u003e Note: If not used immediately, it can be used in  2 - 8°C  Store for up to  2  months. Alternatively, repackage and  -20°C  Lower storage. Do not exceed the expiration date indicated on the label. After thawing the aliquot, use immediately or in  2 - 8°C  Store for up to  2  Zhou, do not refreeze. \u003c\/p\u003e\n\u003cp\u003e will  20 μL  supplements A join  1.98 mL  cold  (2 - 8°C) Basic medium 1 middle. After thorough mixing, store in  2 - 8°C 。 \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e3 Differentiation into endoderm cells ( DE )(with 24 (Taking orifice plates as an example) \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e Add to passage cells to 24 The operation of the orifice plate, when the convergence degree reaches 85%-90% Proceed at DE Differentiation, recorded as the first 0 heaven. \u003c\/p\u003e\n\u003cp\u003e （ 1 )th  0  Day: Preheating  (37°C)  No  0  Daily requirements  DE  medium volume  (0.7 mL\/ hole ) 。 The remaining  DE  The culture medium is stored in  2 - 8°C 。 \u003c\/p\u003e\n\u003cp\u003e From  hPSC Absorbed into cells   Complete training. Add dropwise to each well of the tilted culture plate along the well wall.  0.7 mL DE  culture medium. In  37°C 、 5% CO₂  and  95%  Most incubated under humidity  24  Hours. \u003c\/p\u003e\n\u003cp\u003e （ 2 )th  1  Day: Preheating  (37°C)  No  1  Required for daily use  DE  medium volume  (0.5 mL\/ hole ) 。 The remaining  DE  The culture medium is stored in  2 - 8°C 。 The culture medium is aspirated from the cells. Add dropwise to each well of the tilted culture plate along the well wall.  0.5 mL DE  culture medium. In  37°C 、 5% CO₂  and  95%  Incubation under humidity  24  Hours. \u003c\/p\u003e\n\u003cp\u003e （ 3 )th  2  Day: Preheating (37°C)  The remaining  DE  culture medium. The culture medium is aspirated from the cells. Add dropwise to each well of the tilted culture plate along the well wall.  0.5 mL DE  culture medium. In  37°C 、 5% CO₂  and  95%  Incubation under humidity  24  Hours. \u003c\/p\u003e\n\u003cp\u003e （ 4 )th  3  Day: Cells can detect the formation of definitive endoderm. Multiporous cells can continue to differentiate into middle and hindgut spheres ( MH ）。 \u003c\/p\u003e\n\u003cp\u003e Note: During definitive endoderm induction, cells appear to undergo massive cell death. Shorten the cell life as much as possible  37°C  Time outside the incubator. pass  24  After hours of definitive endoderm induction, the cells are very sensitive and require careful medium changes. incubation  72  After hours, a confluent monolayer of closely aligned endoderm cells will form. \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e4 ,middle \/ hindgut  (MH) Preparation of culture medium \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e （ 1 ) in the  3  God, prepare the first  3 - 8  Daily requirements  MH  Culture medium (basic medium 2+ supplements  B + supplements  C ) volume ( 3 mL\/ hole). \u003c\/p\u003e\n\u003cp\u003e （ 2 ) to prepare  3 mL MH  Taking the culture medium as an example: \u003c\/p\u003e\n\u003cp\u003e Thaw replenishment on ice  B  and  C 。 After mixing in basic culture medium 2 Previously kept on ice. Mix thoroughly. \u003c\/p\u003e\n\u003cp\u003e Note: If not used immediately, repackage and store in  -20°C  Lower storage. Do not exceed the shelf life of the supplement. After thawing aliquots, use immediately, do not refreeze. \u003c\/p\u003e\n\u003cp\u003e will  30 µL  supplements  B and  30 µL  supplements  C  join  2.94 mL  cold  (2 - 8°C) Basic medium 2 middle. Mix well and store in  2 - 8°C 。 \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e5 Endoderm cells differentiate into middle \/ hindgut  (MH)\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e （ 1 )th  3  Day: Preheating (15 - 25°C)  sufficient  MH  culture medium  (0.5 mL\/ hole ) 。 The remaining  MH  The culture medium is stored in  2 - 8°C 。 \u003c\/p\u003e\n\u003cp\u003e （ 2 Remove the culture medium from the endoderm cells and replace it with...  0.5 mL MH  culture medium. In  37°C 、 5% CO2 and  95%  Incubation under humidity  24  Hours. \u003c\/p\u003e\n\u003cp\u003e （ 3 )th  4 - 8  Day: Every  24  Complete media replacement was performed every hour and spheroids were evaluated, culturing the midgut. \/ Posterior intestinal sphere. \u003c\/p\u003e\n\u003cp\u003e Note: Ensure that the culture is removed.  30  Return to the incubator within minutes. \u003c\/p\u003e\n\u003cp\u003ea.  The monolayers were observed under a microscope. As early as the differentiation stage  4  It may be observed in the sky.  3D  Structure. The free-floating middle and hindgut spheroids will be in the first stage of differentiation.  5 - 8  The sky appeared. \u003c\/p\u003e\n\u003cp\u003eb.  Use  1 mL  Pipette, suction from the cells  0.5 mL  Culture medium, transferred to sterile  24  The wells were in clear flat-bottomed plates to assess the number and concentration of middle and posterior intestinal spheroids released from the cell monolayer. \u003c\/p\u003e\n\u003cp\u003ec.  Add to cells  0.5 mL  fresh  MH  culture medium. In  37°C 、 5% CO2 and  95%  Incubate under humidity. \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Note: The timing of peak midgut spheroid production may vary.  hPSC  It varies by cell line. To obtain reproducible experimental results, middle and hindgut spheroids generated on the same day of differentiation should always be used to harvest and initiate human colon organoid cultures. Usually at the stage of differentiation  8  The largest number of sprouts was observed on that day. \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e （ 4 On the day of spheroid embedding: Using a pipette, add the spheroid suspension from each well to a new one.  24  Counting was performed in one well of the well plate. Approximately  70-100  Corresponding volume addition of free floating spheres (based on previous sphere count)  15 mL  In a conical tube. Culture of intestinal organoids was performed. \u003c\/p\u003e\n\u003cp\u003e Note: The middle and posterior intestinal spheroid is the diameter  ≥ 75 µm  The cell aggregates have the potential to produce a human intestinal organoid. Multiple fused spheroids should be counted as a unit that will generate a human intestinal organoid. The remaining monolayer cultures can be used to detect the formation of the midgut or further differentiate in subsequent days to embed more spheroids. \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e6 Human colon organoid medium  (HCO)  Preparation of \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e （ 1 Prepare the required colon  HCO  Culture medium (basic medium 3+ supplements D+ supplements E+ supplements F ) volume ( 2 mL\/ hole). \u003c\/p\u003e\n\u003cp\u003e （ 2 ) to prepare  100 mL Colon  HCO  Taking the culture medium as an example: \u003c\/p\u003e\n\u003cp\u003e Thaw replenishment on ice D , supplements E , supplements F 。 After mixing in basic culture medium 3 Previously kept on ice. Mix thoroughly. \u003c\/p\u003e\n\u003cp\u003e Note: If not used immediately, repackage and store in  2-8°C  Store below, you can save 6 Zhou. Do not exceed the shelf life of the supplement. After thawing aliquots, use immediately, do not refreeze. \u003c\/p\u003e\n\u003cp\u003e will  100μL  supplements  D 、 50 µL  supplements  E and  2mL  supplements  F join  98 mL  cold  (2 - 8°C) Basic medium 3 middle. Mix well and store in  2 - 8°C 。 \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e7 Embedding the spheroid in  Matrigel middle \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e （ 1 The low growth factor matrix gel was subpackaged and frozen for storage (each aliquot of reagent). 200μL  For 4 (a sample). \u003c\/p\u003e\n\u003cp\u003e （ 2 Thaw an aliquot of low growth factor matrix gel on ice (each sample requires...).  50 µL  Growth factor reduced matrix glue). A box of sterile  100 µL  The pipette tip is placed in  -20°C 。 \u003c\/p\u003e\n\u003cp\u003e （ 3 ) and other collected MH The spheroids settled to the bottom of the conical tube, and the supernatant was carefully aspirated and discarded. \u003c\/p\u003e\n\u003cp\u003e （ 4 Add to the spheroid 1 mL Contains 15 mM HEPES  of DMEM\/F-12 。 At room temperature  (15 - 25°C)  Below  300xg  centrifuge  5  minutes. \u003c\/p\u003e\n\u003cp\u003e （ 5 ) Use  1 mL  Pipette, carefully aspirate and discard the supernatant. \u003c\/p\u003e\n\u003cp\u003e Note: Remove the supernatant as much as possible. \u003c\/p\u003e\n\u003cp\u003e （ 6 The thawed matrix gel and cell suspension were placed on an ice box and aspirated using a precooled gun tip. 50μL Mix the matrix gel with the cell suspension, taking care to avoid bubbles. \u003c\/p\u003e\n\u003cp\u003e （ 7 Using a pre-cooled gun tip, press each hole. 50μL Seed the cell-containing matrix gel into 24 On the well plate, when the mixture is slowly injected, the gun tip is gradually moved upwards so that the cells are evenly distributed in the glue. \u003c\/p\u003e\n\u003cp\u003e （ 8 Place the culture plate in an incubator. 20-30min Solidifying the gel droplets; \u003c\/p\u003e\n\u003cp\u003e （ 9 ) Ice-thawed colon organoid supplement G Keep on ice and mix well. take 5 mL Colon ( HCO Culture medium addition 5 μL colon organoid supplement G Mix thoroughly. Per hole 500 μL Add to the edge of each hole, taking care not to disturb the hole cover.  37°C 、 5% CO₂  and  95%  Cultivation was carried out under humidity. \u003c\/p\u003e\n\u003cp\u003e Note: Colon organoid supplements G It needs to be configured and used immediately, paying attention to the amount used each time before configuring. \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e8 Replace human colon organoid culture medium ( HCO ） \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e （ 1 ） 3 Queen, do it once HCO Culture medium (without supplement G Replace ) and add new ones to each culture well. 500μL HCO Culture medium (without supplement G ), in  37°C 、 5% CO₂  and  95%  Cultivation was carried out under humidity. \u003c\/p\u003e\n\u003cp\u003e （ 2 ) Weekly 3 The culture medium is replaced secondarily, and every other 10-14 Transfer the organoid culture to a new medium containing matrix gel. When replacing, the appropriate passage ratio should be followed (approximately [number missing] per dish). 80-100 (The number of cells in each organ) is used to perform the operation. \u003c\/p\u003e\n\u003cp\u003e Note: After the first passage, antibiotics (e.g.  50μg\/ml  Gentamicin) was added to the medium \u003c\/p\u003e\n\u003cp\u003e （ 3 ) After the 3 After the second passage, the colorectal tissue cell mass should have been completely formed before identification and related experiments can be carried out. \u003c\/p\u003e\n\u003cp\u003e Note: After multiple passages, the cell growth rate may decrease, which may be due to a decrease in the number of mesenchymal cells and stem cells. \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e This product is hPSC Colon organoid kit for inducing differentiation, human pluripotent stem cells hPSC Induction of differentiation by the kit yields intestinal organoids composed of colon-associated cells that express human colon organoid-specific markers, including colon transcription factors SATB2 、 α- carbonic anhydrase  II (CA-II) 、 α- carbonic anhydrase  IV (CA-IV) goblet cell marker mucin, -2 (MUC2)  and mucin -5B (MUC5B) , and the back  HOX  Expression of genes, etc. \u003cbr\u003e This product requires operators to have hPSC Experience in cell culture and a certain understanding of organoids. \u003cbr\u003e\u003cstrong\u003e\u003cstrong\u003e Product Composition: \u003cbr\u003e\u003c\/strong\u003e\u003c\/strong\u003e\u003ctable style=\"border-collapse: collapse; width: 100%; height: 240px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.8882%; height: 22px;\"\u003e\u003cstrong\u003e Research Phase \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.8882%; height: 22px;\"\u003e\u003cstrong\u003e Product Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.8882%; height: 22px;\"\u003e\u003cstrong\u003e Product Specifications \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.8882%; height: 22px;\"\u003e\u003cstrong\u003e store \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 44px; width: 23.8882%;\" rowspan=\"2\"\u003e definitive endoderm (DE) Stage \u003c\/td\u003e\n\u003ctd style=\"width: 23.8882%;\" align=\"center\" valign=\"middle\" width=\"157\"\u003e\u003cp\u003e Basic medium 1\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.8882%;\" align=\"center\" valign=\"middle\" width=\"99\"\u003e\u003cp\u003e40mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.8882%;\" align=\"center\" valign=\"middle\" width=\"169\"\u003e\u003cp\u003e-20℃ ， 12 Month \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 23.8882%;\" align=\"center\" valign=\"middle\" width=\"157\"\u003e\u003cp\u003e supplements A\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.8882%;\" align=\"center\" valign=\"middle\" width=\"99\"\u003e\u003cp\u003e1100μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.8882%;\" align=\"center\" valign=\"middle\" width=\"169\"\u003e\u003cp\u003e-20℃ ， 6 Month \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.8882%; height: 73px;\" rowspan=\"3\"\u003e Middle and posterior intestinal spheres ( MH Differentiation stage \u003c\/td\u003e\n\u003ctd style=\"width: 23.8882%;\" align=\"center\" valign=\"middle\" width=\"157\"\u003e\u003cp\u003e Basic medium 2\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.8882%;\" align=\"center\" valign=\"middle\" width=\"99\"\u003e\u003cp\u003e60mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.8882%;\" align=\"center\" valign=\"middle\" width=\"169\"\u003e\u003cp\u003e-20℃ ， 12 Month \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 23.8882%;\" align=\"center\" valign=\"middle\" width=\"157\"\u003e\u003cp\u003e supplements B\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.8882%;\" align=\"center\" valign=\"middle\" width=\"99\"\u003e\u003cp\u003e640μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.8882%;\" align=\"center\" valign=\"middle\" width=\"169\"\u003e\u003cp\u003e-20℃ ， 6 Month \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 23.8882%;\" align=\"center\" valign=\"middle\" width=\"157\"\u003e\u003cp\u003e supplements C\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.8882%;\" align=\"center\" valign=\"middle\" width=\"99\"\u003e\u003cp\u003e640μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.8882%;\" align=\"center\" valign=\"middle\" width=\"169\"\u003e\u003cp\u003e-20℃ ， 6 Month \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 23.8882%; height: 101px;\" rowspan=\"5\"\u003e Colon ( 3D ) Organoid stage \u003cbr\u003e\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 23.8882%;\" align=\"center\" valign=\"middle\" width=\"157\"\u003e\u003cp\u003e Basic medium 3\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.8882%;\" align=\"center\" valign=\"middle\" width=\"99\"\u003e\u003cp\u003e200mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.8882%;\" align=\"center\" valign=\"middle\" width=\"169\"\u003e\u003cp\u003e-20℃ ， 12 Month \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 23.8882%;\" align=\"center\" valign=\"middle\" width=\"157\"\u003e\u003cp\u003e supplements D\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.8882%;\" align=\"center\" valign=\"middle\" width=\"99\"\u003e\u003cp\u003e200μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.8882%;\" align=\"center\" valign=\"middle\" width=\"169\"\u003e\u003cp\u003e-20℃ ， 6 Month \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 23.8882%;\" align=\"center\" valign=\"middle\" width=\"157\"\u003e\u003cp\u003e supplements E\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.8882%;\" align=\"center\" valign=\"middle\" width=\"99\"\u003e\u003cp\u003e100μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.8882%;\" align=\"center\" valign=\"middle\" width=\"169\"\u003e\u003cp\u003e-20℃ Protect from light. 12 Month \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 23.8882%;\" align=\"center\" valign=\"middle\" width=\"157\"\u003e\u003cp\u003e supplements F\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.8882%;\" align=\"center\" valign=\"middle\" width=\"99\"\u003e\u003cp\u003e4mL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.8882%;\" align=\"center\" valign=\"middle\" width=\"169\"\u003e\u003cp\u003e-20℃ ， 6 Month \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 23.8882%;\" align=\"center\" valign=\"middle\" width=\"157\"\u003e\u003cp\u003e supplements G\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.8882%;\" align=\"center\" valign=\"middle\" width=\"99\"\u003e\u003cp\u003e200μL\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.8882%;\" align=\"center\" valign=\"middle\" width=\"169\"\u003e\u003cp\u003e-20℃ ， 3 Month \u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch3\u003e \u003c\/h3\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eSee product composition for details.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"1Kit","offer_id":43224460918859,"sku":"abs90484-1Kit","price":4127.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.antbioinc.com\/products\/hpsc-derived-colon-organoid-kit-abs90484","provider":"AntBio","version":"1.0","type":"link"}