{"product_id":"goat-tnfa-elisa-kit-abs554759","title":"Goat TNFa ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e I. Self-provided test equipment required for the experiment: \u003c\/strong\u003e\u003cbr\u003e1 Standard plate reader (including wavelength) 450nm ） \u003cbr\u003e2 High speed centrifuge \u003cbr\u003e3 Electric heating incubator \u003cbr\u003e4 Mixer \u003cbr\u003e5 High-precision pipettes, EP Tubes and disposable tips ( 0.5-10uL 、 2-20uL 、 20-200uL 、 200-1000uL ） \u003cbr\u003e6 Double distilled water or deionized water, volumetric flasks, absorbent paper, etc. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e II. Sample Collection, Preparation and Storage: \u003c\/strong\u003e\u003cbr\u003e1 Serum: Whole blood samples were kept at room temperature. 2 Hours or 4℃ After overnight 1000×g centrifuge 20 Minutes, take the supernatant for detection. Blood collection tubes should be disposable, heat-free, and endotoxin-free tubes. deposit -20℃ or -80℃ For storage, avoid repeated freezing and thawing. \u003cbr\u003e2 Plasma: The sample after collection. 30 Within minutes 2-8℃ 、 1000×g centrifuge 15 Minutes, take the supernatant for detection. Recommended use of anticoagulants EDTA-Na2 Avoid using hemolytic and hyperlipidemic samples. deposit -20℃ or -80℃ For storage, avoid repeated freezing and thawing. \u003cbr\u003e3 Tissue homogenization: Take an appropriate amount of tissue pieces and place them in the pre-cooled... PBS （ 0.01M ， pH7.0-7.2 The blood is cleaned and removed in the homogenate (lysed red blood cells in the homogenate will affect the measurement results). After weighing, the tissue is shredded and then combined with the corresponding volume. PBS (Generally according to 1:9 The mass-to-volume ratio can be adjusted appropriately according to experimental needs, and records should be kept. It is recommended to... PBS China joined 1% Protease inhibitors) were added to a glass homogenizer and thoroughly ground on ice; To further lyse tissue cells, the homogenate can be subjected to ultrasonic disruption or repeated freeze-thaw treatment (pay attention to ice bath cooling during ultrasonic disruption, and the repeated freeze-thaw method can be repeated). 2 Next). Finally, the prepared homogenate is placed in... 5000×g centrifuge 5-10 Minutes, take the supernatant for detection. \u003cbr\u003e4 Cell culture supernatant: Take the cell supernatant from 1000×g centrifuge 20 Minutes to remove impurities and cell debris. The supernatant can be taken for detection and placed in -20℃ or -80℃ Store, but avoid repeated freezing and thawing. \u003cbr\u003e5 Urine: Please collect your first urine in the morning (middle urine), or  24 Hourly urine, 2000×g  centrifuge  15  Collect the supernatant after 10 minutes and store the sample   At -20℃ And repeated freezing and thawing should be avoided. \u003cbr\u003e6 Saliva: Collect samples using a saliva sample collection tube, and then...  2-8℃,1000×g  centrifuge  15  The supernatant can be taken for testing within minutes, or it can be repackaged and then... -20℃  Save. Avoid repeated freezing and thawing. \u003cbr\u003e7 Other biological samples: Please 1000×g centrifuge 20 Minutes, take the supernatant for detection. \u003cbr\u003e\u003cstrong\u003e Note: \u003c\/strong\u003e\u003cbr\u003e The sample should be clear and transparent, and the suspended matter should be removed by centrifugation. Hemolysis of the sample will affect the results, so hemolyzed samples should not be used. \u003cbr\u003e If after sample collection 1 Tests performed within a week can be stored at 4℃ If testing is not possible in time, please repackage according to the single-use amount and freeze it. -20℃ （ 1  Testing within a month), or -80℃ （ 3-6 (Testing within the month) to avoid repeated freezing and thawing. Please allow the sample to stand at room temperature before the experiment. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e III. Sample dilution protocol: \u003c\/strong\u003e\u003cbr\u003e If your test sample needs to be diluted, the general dilution principles are referenced below: \u003cbr\u003e1 Dilution 50 Double: one-step dilution. take 5uL Sample to 245uL Standard \u0026amp; In the sample dilution, is 50 fold dilution; \u003cbr\u003e2 Dilution 100 Double: one-step dilution. take  5uL Sample to 495uL Standard \u0026amp; In the sample dilution, is 100 fold dilution; \u003cbr\u003e3 Dilution 1000 Double: Two-step dilution. take  5uL Sample to 95uL Standard \u0026amp; In the sample dilution, is 20 Dilute twice, then take  5uL 20  Fold the diluted sample to 245uL Standard \u0026amp; In the sample dilution, is 50 Double dilution, co-dilution 1000 times; \u003cbr\u003e4 Dilution 100000 Double: Three-step dilution. take 5uL Sample to 195uL Standard \u0026amp; In the sample dilution, is 40 Dilute twice, then take 5 uL 40  Fold the diluted sample to 245uL Standard \u0026amp; In the sample dilution, do 50 Dilute twice, and finally take... 5uL2000 Fold the diluted sample to 245uL Standard \u0026amp; In the sample dilution, do 50 Fold dilution, total dilution 100000 times; \u003cbr\u003e5 The amount of liquid taken during each dilution step shall not be less than 3uL Dilution factor not exceeding 100 Double. Too small a sample size can easily cause greater errors during the mixing process. Each dilution step must be mixed evenly to avoid foaming. \u003cbr\u003e6 In cases where the dilution factor is very high, it can be used first. PBS Dilution, final step using the standard from the kit \u0026amp; Sample dilution. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e IV. Pre-testing preparations: \u003c\/strong\u003e\u003cbr\u003e1 Please advance 30 Remove the kit from the refrigerator and allow to equilibrate to room temperature. \u003cbr\u003e2 Use double distilled water to 25× The concentrated wash solution was diluted into 1× Return any unused working fluid. 4℃ 。 \u003cbr\u003e3 Standard: Add standard \u0026amp; sample universal diluent 1.0mL Transfer to the lyophilized standard, tighten the tube cap, and let stand. 10 After it has fully dissolved, gently mix well (concentration is...). 1000pg\/mL ）。 Then it was diluted in a double ratio to... 1000pg\/mL ， 500pg\/mL ， 250 pg\/mL ， 125pg\/mL ， 62.5pg\/mL ， 31.25pg\/mL ， 15.63pg\/mL  Standard dilution ( 0pg\/mL ) is a blank hole. Prepare the standards for use according to the amount you need. It is recommended that the configured standards be 15 Add the sample within minutes; it is not recommended to leave it for too long. \u003cbr\u003e\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20261009\/a34815e3cf064a2d984ab6cdb50dd508.jpg\" alt=\"\" width=\"616\" height=\"256\"\u003e\u003c\/div\u003e4 Biotinylated antibody working solution: Calculate the amount required for this experiment before the experiment (according to 100uL\/ Hole meters should be configured multiple times during actual configuration. 100-200 uL ), before use 15 Minutes, dilute the concentrated biotinylated antibody with biotinylated antibody diluent ( 1:100 The working concentration is reached and used on the same day. Dilution principle is taken 1uL Concentrated biotinylated antibody added to 99uL In the biotinylated antibody dilution, mix well with a pipette. \u003cbr\u003e5 Enzyme conjugate working solution: Calculate the amount required for this experiment before the experiment (according to 100uL\/ Hole meters should be configured multiple times during actual configuration. 100-200uL ）。 Before use 15 Dilute the concentrate with enzyme conjugate diluent for minutes. HRP Enzyme conjugate ( 1:100 The working concentration is reached and used on the same day. Dilution principle is taken 1uL The concentrated enzyme conjugate was added to 99uL In the enzyme conjugate dilution, mix well with a pipette. \u003cbr\u003e6 、 TMB substrate —— Draw the required dose of solution with a pipette and do not pour the residual solution back into the vial. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e V. Operating Steps: \u003c\/strong\u003e\u003cbr\u003e1 Before the experiment begins, all reagents should be equilibrated to room temperature and all reagents should be prepared in advance. Calculate the number of samples that may be used throughout the test and reserve sufficient samples in advance. When diluting reagents or samples, they must be mixed well, and foaming should be avoided as much as possible during mixing. If the sample concentration is too high, dilute it with sample diluent to make the sample within the detection range of the kit. \u003cbr\u003e2 Add standard or sample to be tested 100uL (If the sample needs to be diluted, please refer to the sample dilution principle for the dilution method). Note that there are no air bubbles. When adding the sample, add the sample to the bottom of the enzyme label plate well, trying not to touch the well wall. Gently shake and mix well. Cover or cover the enzyme label plate. 37℃ incubation 80 minutes. To ensure the validity of the experimental results, please use a new standard solution for each experiment. \u003cbr\u003e3 Discard the liquid inside the well, spin dry, and wash the plate. 3 Next. Each hole 200uL Washing with detergent, soaking 1-2 For minutes, shake off the liquid from the enzyme label plate (or wash the plate using a plate washer). After the last wash was completed, the enzyme label plate was patted dry on absorbent paper. \u003cbr\u003e4 Add biotin antibody working solution to each well 100uL (Can be done in advance) 15 (Prepared in minutes), the enzyme label plate is coated with a film. 37℃ incubation 50 minutes. \u003cbr\u003e5 Discard the liquid inside the hole and wash the plate. 3 Next. Each hole 200uL Washing with detergent, soaking 1-2 For minutes, shake off the liquid from the enzyme label plate (or wash the plate using a plate washer). After the last wash was completed, the enzyme label plate was patted dry on absorbent paper. \u003cbr\u003e6 Add enzyme conjugate working solution to each well 100uL (Can be done in advance) 15 (Prepared in minutes) 37℃ incubation 50 minutes. \u003cbr\u003e7 Discard the liquid inside the hole and wash the plate. 5 Next. Each hole 200uL Washing with detergent, soaking 1-2 For minutes, shake off the liquid from the enzyme label plate (or wash the plate using a plate washer). After the last wash was completed, the enzyme label plate was patted dry on absorbent paper. \u003cbr\u003e8 Add to each well TMB chromogenic substrate solution 90uL ， 37℃ Incubation in the dark 20 Minutes (shortened or extended as appropriate depending on actual color development, but not exceeding 30 minutes). \u003cbr\u003e9 Add stop solution to each well 50uL The reaction is terminated (at which point the blue color immediately turns yellow). The order of addition of the stop solution should be as similar as possible to the order of addition of the color developer. To ensure the accuracy of the experimental results, the stop solution should be added as soon as possible after the substrate reaction time expires. \u003cbr\u003e10 Immediately use a microplate reader. 450nm Measure the optical density value of each hole at the wavelength ( OD value). The instrument should be preheated before use, and the testing procedure should be set. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e VI. Calculation of Experimental \u003c\/strong\u003e\u003cbr\u003e1 For each standard and sample OD The value should be subtracted from the blank hole. OD Value. If multiple holes are set, the average value should be used for calculation. \u003cdiv\u003e2 For ease of calculation, although concentration is an independent variable... OD The value is a dependent variable; we will still use the standard when drawing. OD Value as abscissa ( X Axis), and the concentration of the standard is on the ordinate ( Y axis). Also, for the intuitiveness of the test results, the figures provide raw data rather than logarithmic values. Due to differences in experimental operating conditions (such as operator, pipetting technique, plate washing technique, and temperature conditions), the standard curve... OD The values will vary. The standard curves provided are for reference only, and experimenters need to establish standard curves based on their own experiments. Used samples OD The value can be calculated from the standard curve to calculate the sample concentration, and then multiplied by the dilution factor, which is the actual concentration of the sample. It is recommended to use professional curve drawing software, such as curveexpert 。 \u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20261009\/5c397bbf45e8427ca8ca2af26e0fc72d.jpg\" alt=\"\" width=\"439\" height=\"245\"\u003e\u003cstrong\u003e\u003cbr\u003e VII. Kit performance: \u003c\/strong\u003e\n\u003c\/div\u003e1 , Precision: \u003cbr\u003e in-plate precision ( Precision within the assay ):CV%\u0026lt;8%\u003cbr\u003e Three samples with known concentrations were respectively 1 Enzyme-labeled plate test 20 Times to evaluate the in-plate precision of the assay. \u003cbr\u003e Interplate precision ( Determination of inter-plate precision ):CV%\u0026lt;10%\u003cbr\u003e Three samples with known concentrations were respectively 3 Tested on several different enzyme-labeled plates 40 This was performed several times to evaluate the precision of the analytical plate. \u003cbr\u003e2 Recovery: \u003cbr\u003e Add known concentrations of goats to different samples respectively.  TNFa The recovery rate experiment was conducted to obtain the recovery range and average recovery rate. \u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 95%; height: 88px; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 32.2148%; height: 22px; text-align: center;\"\u003e\u003cstrong\u003e Sample Type \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 32.2148%; height: 22px; text-align: center;\"\u003e\u003cstrong\u003e Recovery range \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 32.2148%; height: 22px; text-align: center;\"\u003e\u003cstrong\u003e average recovery \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 32.2148%; height: 22px; text-align: center;\"\u003e serum (n=5)\u003c\/td\u003e\n\u003ctd style=\"width: 32.2148%; height: 22px; text-align: center;\"\u003e78-95%\u003c\/td\u003e\n\u003ctd style=\"width: 32.2148%; height: 22px; text-align: center;\"\u003e86%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 32.2148%; height: 22px; text-align: center;\"\u003eEDTA  plasma (n=5)\u003c\/td\u003e\n\u003ctd style=\"width: 32.2148%; height: 22px; text-align: center;\"\u003e80-92%\u003c\/td\u003e\n\u003ctd style=\"width: 32.2148%; height: 22px; text-align: center;\"\u003e86%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 32.2148%; text-align: center; height: 22px;\"\u003eheparin  plasma (n=5)\u003c\/td\u003e\n\u003ctd style=\"width: 32.2148%; text-align: center; height: 22px;\"\u003e80-96%\u003c\/td\u003e\n\u003ctd style=\"width: 32.2148%; text-align: center; height: 22px;\"\u003e88%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e3 Linear dilution: \u003cbr\u003e Goats will be added  TNFa Dilute the samples separately 2 times, 4 times, 8 times, 16 Recovery experiments were conducted to determine the recovery range. \u003ctable style=\"border-collapse: collapse; width: 95%; height: 64px; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 18.2105%; height: 22px; text-align: center;\"\u003e\u003cstrong\u003e Sample Type \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.9506%; height: 22px; text-align: center;\"\u003e\u003cstrong\u003e1:2\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 20.042%; height: 22px; text-align: center;\"\u003e\u003cstrong\u003e1:4\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.3616%; height: 22px; text-align: center;\"\u003e\u003cstrong\u003e1:8\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.4533%; height: 22px; text-align: center;\"\u003e\u003cstrong\u003e1:16\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 10px; width: 18.2105%; text-align: center;\"\u003e serum (n=5)\u003c\/td\u003e\n\u003ctd style=\"width: 19.9506%; height: 10px; text-align: center;\"\u003e91-99%\u003c\/td\u003e\n\u003ctd style=\"width: 20.042%; height: 10px; text-align: center;\"\u003e83-89%\u003c\/td\u003e\n\u003ctd style=\"width: 19.3616%; height: 10px; text-align: center;\"\u003e88-94%\u003c\/td\u003e\n\u003ctd style=\"width: 19.4533%; height: 10px; text-align: center;\"\u003e87-93%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 18.2105%; height: 22px; text-align: center;\"\u003eEDTA  plasma (n=5)\u003c\/td\u003e\n\u003ctd style=\"width: 19.9506%; height: 22px; text-align: center;\"\u003e88-97%\u003c\/td\u003e\n\u003ctd style=\"width: 20.042%; height: 22px; text-align: center;\"\u003e86-92%\u003c\/td\u003e\n\u003ctd style=\"width: 19.3616%; height: 22px; text-align: center;\"\u003e94-103%\u003c\/td\u003e\n\u003ctd style=\"width: 19.4533%; height: 22px; text-align: center;\"\u003e89-97%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 10px; width: 18.2105%; text-align: center;\"\u003eheparin  plasma (n=5)\u003c\/td\u003e\n\u003ctd style=\"width: 19.9506%; height: 10px; text-align: center;\"\u003e85-94%\u003c\/td\u003e\n\u003ctd style=\"width: 20.042%; height: 10px; text-align: center;\"\u003e97-106%\u003c\/td\u003e\n\u003ctd style=\"width: 19.3616%; height: 10px; text-align: center;\"\u003e82-95%\u003c\/td\u003e\n\u003ctd style=\"width: 19.4533%; height: 10px; text-align: center;\"\u003e93-105%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e5.9pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecies Reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eGoat\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis kit adopts the principle of sandwich method. Specific anti-goat TNFa antibodies were coated in 96-well microplates, goat TNFa standards or samples were added to the microwells respectively, the goat TNFa protein in the standards or the goat TNFa protein in the samples were bound to the anti-goat TNFa antibodies immobilized on the microplates, then biotinylated anti-goat TNFa antibodies were added, the unbound biotinylated antibodies were washed, HRP-labeled streptavidin was added, and after thorough washing again, TMB substrate was added for color development. TMB turns blue under peroxidase catalysis and eventually yellow under the action of acid. The depth of color was positively correlated with the goat TNFα protein in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the sample concentration was calculated by plotting a standard curve.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eGoat Tumor Necrosis Factor Alpha ELISA Kit;DIF; TNF-A; TNFSF2; Cachectin; Tumor Necrosis Factor Ligand Superfamily Member 2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDetection Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003esandwich method\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"border-collapse: collapse; width: 96.0313%; height: 163px; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cstrong\u003e Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cstrong\u003e96T configuration \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 14.99%; text-align: center;\" width=\"29.9800%\"\u003e\u003cstrong\u003e Storage conditions \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e Enzyme label plate (removable) \u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e12 strip ×8 hole \u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 14.99%; text-align: center;\" width=\"29.9800%\"\u003e4℃\/-20℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e lyophilized standard \u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 14.99%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv style=\"text-align: center;\"\u003e4℃\/-20℃\u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e Standard \u0026amp; Sample dilution \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e20mL\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 14.99%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e4℃\/-20℃\u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e Concentrated biotinylated antibodies ( 100× ） \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e120uL\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 14.99%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e4℃\/-20℃\u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e biotinylated antibody dilution \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e12mL\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 14.99%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e4℃\/-20℃\u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e concentrate HRP Enzyme conjugate ( 100× ） \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e120uL\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 14.99%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e4℃\/-20℃\u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e enzyme conjugate dilution \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e12mL\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 14.99%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e4℃\/-20℃\u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e Concentrated washing solution ( 25× ） \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e20mL\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 14.99%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e4℃\/-20℃\u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px; text-align: center;\"\u003e\n\u003ctd style=\"height: 10px; width: 31.98%; text-align: center;\" width=\"31.9800%\"\u003e\u003cdiv\u003e Chromogenic substrate solution ( TMB ） \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 19%; text-align: center;\" width=\"19.0000%\"\u003e\u003cdiv\u003e10mL\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 14.99%; text-align: center;\" width=\"29.9800%\"\u003e\u003cdiv\u003e4℃\/-20℃( (Protected from light) \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.98%; text-align: center; height: 22px;\"\u003e\u003cdiv\u003e reaction stopping solution \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19%; text-align: center; height: 22px;\"\u003e\u003cdiv\u003e6mL\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 14.99%; text-align: center; height: 22px;\"\u003e\u003cdiv\u003e4℃\/-20℃\u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 31.98%; text-align: center; height: 19px;\"\u003e\u003cdiv\u003e sealing plate coating \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19%; text-align: center; height: 19px;\"\u003e\u003cdiv\u003e2\u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 14.99%; text-align: center; height: 19px;\"\u003e\u003cdiv\u003e normal temperature \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 31.98%; text-align: center; height: 22px;\"\u003e\u003cdiv\u003e Product instructions \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19%; text-align: center; height: 22px;\"\u003e\u003cdiv\u003e1 portion \u003c\/div\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 14.99%; text-align: center; height: 22px;\"\u003e\u003cdiv\u003e normal temperature \u003c\/div\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1 If the entire kit is stored in -20°C Please place the reagent kit the night before the experiment. 4°C 。 \u003cbr\u003e2 Salt will precipitate when the concentrated washing liquid is stored at low temperature. When diluted, it can be heated in a water bath to help dissolve. \u003cbr\u003e3 The newly opened enzyme label plate wells may contain a small amount of water-like substance, which is normal and will not affect the experimental results. \u003cbr\u003e4 All kit components have undergone formulation and quality control testing to successfully function as a kit. Do not mix or substitute reagents or materials from other kits; performance cannot be guaranteed if used alone or substituted. \u003cbr\u003e5 This kit is for laboratory research and development purposes only and is not intended for use in humans or animals. \u003cbr\u003e6 Reagents should be treated as hazardous substances, and should be handled carefully and properly. \u003cbr\u003e7 Gloves, lab gowns, and protective glasses should always be worn to avoid skin and eye contact with the termination solution. TMB 。 In case of accidental contact, please rinse thoroughly with water. \u003cbr\u003e8 Before using the kit, please ensure that all components are dissolved and mixed well. If the reconstituted standard is not used up, discard it. \u003cbr\u003e9 Concentrated biotinylated antibodies. The concentrated enzyme conjugate is relatively small in size and may be scattered in various parts of the tube during transportation. Please note this before use. 1000×g  centrifuge 1 Minutes to allow the liquid from the tube wall or bottle cap to settle to the bottom of the tube. Carefully blow with a pipette before use 4-5 Next, mix the solution well. Please prepare the standard, biotinylated antibody working solution, and enzyme conjugate working solution according to the required dosage and use the corresponding diluent to prepare them, and do not confuse them. \u003cbr\u003e10 The concentrated washing liquid taken out of the refrigerator may have crystals, which is normal. You can use a water bath or incubator to completely dissolve the crystals before preparing the washing liquid (the heating temperature should not exceed...). 40°C ）。 The washing solution should be at room temperature during use. \u003cbr\u003e11 Sample addition should be done quickly, and each sample addition should ideally be controlled within the 10 Within minutes, to ensure experimental accuracy, it is recommended to use duplicate wells. When pipetting reagents, a consistent addition sequence is maintained from one well to another,   This will ensure that all wells have the same hatching time. \u003cbr\u003e12 During the washing process, the remaining washing liquid in the reaction hole should be patted dry on absorbent paper. Do not put the filter paper directly into the reaction hole to absorb water. Before reading, be sure to remove any remaining liquid and fingerprints from the bottom to avoid affecting the microplate reader reading. \u003cbr\u003e13 Color developer TMB Direct exposure to strong light should be avoided during storage and use. After adding the substrate, pay attention to the color change in the reaction well. If the gradient is obvious, please stop the reaction in advance to avoid the color being too dark and affecting the reading of the microplate reader. \u003cbr\u003e14 All test tubes and reagents used during the experiment are disposable and are strictly prohibited from being reused, otherwise it will affect the experimental results. \u003cbr\u003e15 During the experiment, please wear a lab coat and latex gloves to take protective measures. In particular, when testing blood or other bodily fluid samples, please comply with the National Biological Laboratory Safety Protection Regulations. \u003cbr\u003e16 Kit components of different batch numbers cannot be mixed (except for washing solution and reaction stopping solution). \u003cbr\u003e17 The enzyme label strips in the kit are removable plates. Please use them in batches according to experimental needs. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eInstructions\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eSerum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUnopened kit, stored at 4℃, shelf life 6 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTest Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e15.63-1000pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":67241212575819,"sku":"abs554759-96T","price":381.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.antbioinc.com\/products\/goat-tnfa-elisa-kit-abs554759","provider":"AntBio","version":"1.0","type":"link"}