{"product_id":"gaster-tissue-digestion-solution-abs9783","title":"Gaster Tissue Digestion Solution","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Before digesting the tissue sample, use ophthalmic scissors or a scalpel to cut the tissue into a volume of approximately 1-3mm.\u003csup\u003e3\u003c\/sup\u003eTissue blocks.\u003cbr\u003e2. Add an appropriate volume of tissue digestive fluid (the volume of digestive fluid should be 5-10 times the volume of the original tissue) according to the size of the original tissue block; Tissue digestion is carried out in a 4 DEG C constant temperature incubator or a constant temperature shaker, and the digestion time is 15-20 minutes.\u003cstrong\u003eNote:\u003c\/strong\u003eThe digestion process must be carefully monitored because excessive digestion may affect cell viability and significantly reduce the success rate of organoid construction. During the digestion process, the digestion suspension can be microscopically examined. After a large number of single cells or cell clusters below 70um are observed under the microscope, the digestion can be considered complete.\u003cbr\u003e3. Add 3 times primary tissue culture buffer to the tissue suspension after confirming the completion of digestion to terminate the digestion.\u003cbr\u003e4. The digestion suspension obtained in the above steps can be directly used for cell separation operations such as centrifugation or screen filtration. Before using the separated cells, the sample must be centrifuged and washed more than twice using organoid buffer before use (recommended centrifugation speed 200-300g for 3 minutes).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eGaster Tissue Digestion Solution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHuman normal gastric primary tissue digestive fluid can quickly and efficiently dissociate tissue samples into cell suspensions or cell masses for the construction of organoids. The tissue digestive fluid is suitable for digestion and dissociation of normal gastrointestinal tissue specimens in vitro primary culture.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20℃ for 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100mL","offer_id":43224142053451,"sku":"abs9783-100mL","price":214.0,"currency_code":"USD","in_stock":true},{"title":"500mL","offer_id":43224142086219,"sku":"abs9783-500mL","price":894.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.antbioinc.com\/products\/gaster-tissue-digestion-solution-abs9783","provider":"AntBio","version":"1.0","type":"link"}