{"product_id":"fixable-viability-dye-452-s0b88801","title":"Fixable Viability Dye 452","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e12 months from date of receipt, -20 °C as supplied\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003eFixable Viability Dye 452 is excited by the Violet laser (with an excitation maximum of 405 nm) and has fluorescence emission maximum at 452 nm, Fixable Viability Dye 452 has similar emission to Brilliant Violet 421™. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eFixable Viability Dye 452 can intracellular diffusion of the dye and covalent binding to higher overall concentrations of amines, \u003c\/span\u003e\u003cspan\u003eEach\u003c\/span\u003e\u003cspan\u003e lot of this product is quality control tested, is useful for discrimination of viable from non-viable mammalian cells in multicolor flow cytometric applications. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eEach lot of this product is quality control tested. The labeled cells can be fixed with formaldehyde for downstream decontamination, freezing and\/or permeabilization and subsequent intracellular staining while maintaining stable viability stain fluorescence.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003e1.\u003c\/span\u003e\u003cspan\u003eWash cells one time in PBS buffer.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.\u003c\/span\u003e\u003cspan\u003eAdd 1 µl of Fixable Viability Dye 452 Stock Solution for each 0.5~1 ml of cell suspension\/PBS (1:500~1:1000) and vortex immediately. It is recommended that the reagent be titrated for optimal performance for each application.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3.\u003c\/span\u003e\u003cspan\u003eResuspend cells at 1*10^7 cells\/ml in Fixable Viability Dye 452, Incubate the mixture for 10-15 minutes at room temperature protected \u003c\/span\u003e\u003cspan\u003efrom light.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e4.\u003c\/span\u003e\u003cspan\u003eWash cells twice with PBS buffer or equivalent buffer containing serum or BSA.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e5.\u003c\/span\u003e\u003cspan\u003eResuspend the cells in Stain Buffer.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-style:italic\"\u003eNote: This protocol applicable to Human PBMCs (human peripheral blood mononuclear cells) Dying, the reagent be titrated for optimal performance for different cell types. Stain, fix and permeabilize cells as desired for downstream applications.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"150μg","offer_id":42302121279563,"sku":null,"price":105.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_ead31099-703f-4a9d-93f7-b753f9444874.png?v=1776073103","url":"https:\/\/www.antbioinc.com\/products\/fixable-viability-dye-452-s0b88801","provider":"AntBio","version":"1.0","type":"link"}