{"product_id":"endo-beta-n-acetylglucosaminidase-endos2-mutant-ua070139","title":"Endo-beta-N-acetylglucosaminidase EndoS2 mutant","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eStreptococcus pyogenes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eEndo-beta-N-acetylglucosaminidase EndoS2、Endoglycosidase S2、EndoS2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eE.coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e93 Da (Reducing)\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e＞95% by SDS-PAGE \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHis Tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e20 mM Tris-HCl、50 mM NaCl、pH 7.5 @ 25°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore at -25 ~ -15℃ for 2 years\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e[1] Sjögren Jonathan, et al. \"EndoS and EndoS2 hydrolyze Fc-glycans on therapeutic antibodies with different glycoform selectivity and can be used for rapid quantification of high-mannose glycans.\" Glycobiology 10:1053-1063.\u003cbr\u003e[2] Li T, et al. \"Glycosynthase Mutants of Endoglycosidase S2 Show Potent Transglycosylation Activity and Remarkably Relaxed Substrate Specificity for Antibody Glycosylation Remodeling. \" J Biol Chem. 2016 Aug 5;291(32):16508-18. \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003eEndoS2 is an endo-β-N-acetylglucosaminidase derived from \u003c\/span\u003e\u003cspan style=\"font-style:italic;color:#000000\"\u003eStreptococcus pyogenes\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e. It hydrolyzes the chitobiose core of N-linked glycans, cleaving between the two innermost GlcNAc residues. A key advantage of EndoS2 is its remarkably broad substrate specificity; unlike the original EndoS, it​ efficiently processes high-mannose, hybrid, and complex-type glycans. This makes it a universal tool for glycan remodeling across various species, including human, mouse, rat, and other mammalian IgGs.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003eTo enhance its synthetic utility, a representative glycosynthase mutant has​ been engineered. This mutation diminishes​ the enzyme's hydrolytic activity while retaining its ability to utilize an​ activated sugar oxazoline donor. This modification shifts the reaction equilibrium overwhelmingly toward synthesis, enabling the production of homogeneous glycoproteins with high yield.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003eThis mutant is​ particularly valuable in therapeutic antibody development. It facilitates the creation of defined glycoforms to enhance effector functions like ADCC. Consequently, the EndoS2 mutant system provides a robust platform for producing next-generation biotherapeutics with improved efficacy.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003eStorage Solution\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e :\u003c\/span\u003e\u003cspan\u003e 2.32\u003c\/span\u003e\u003cspan\u003emg\/mL Endo\u003c\/span\u003e\u003cspan\u003eS2 \u003c\/span\u003e\u003cspan\u003emutant、\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e20 mM Tris-HCl, 50 mM NaCl. (pH 7.5)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003e10*Reaction Buffer\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e：\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e 500 mM sodium acetate\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e \u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e(pH 6.0)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003e1. Combine 100 µg of native IgG, 1 µl of 10*Reaction Buffer and \u003c\/span\u003e\u003cspan\u003ed\u003c\/span\u003e\u003cspan\u003edH\u003c\/span\u003e\u003csub\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/sub\u003e\u003cspan\u003e0 (if necessary) to make a 10 µl total reaction volume.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2. Add 1 µl EndoS2.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3. Incubate reaction at 37°C for 1 hour\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e4\u003c\/span\u003e\u003cspan\u003e. Add 1 µl EndoS2 \u003c\/span\u003e\u003cspan\u003emutant(\u003c\/span\u003e\u003cspan\u003eThe molar ratio of\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003edonor to acceptor was 20:1)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e5. Incubate reaction at 37°C for 1 hour\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eGuidelines\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"margin-bottom: 0px;\"\u003ePlease avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eUnit Definition\u003c\/h4\u003e\u003cdiv\u003eOne unit is defined as the amount of enzyme required to remove \u0026gt; 95% of the carbohydrate from 5 μg of native mouse monoclonal IgG in 1 hour at 37°C in a total reaction volume of 10 µl.\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"100μg","offer_id":43096731385931,"sku":"UA070139-100μg","price":810.0,"currency_code":"USD","in_stock":true},{"title":"500μg","offer_id":43096731418699,"sku":"UA070139-500μg","price":2430.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/1b0af0784a894923947cc3d993b1a4be.png?v=1788242452","url":"https:\/\/www.antbioinc.com\/products\/endo-beta-n-acetylglucosaminidase-endos2-mutant-ua070139","provider":"AntBio","version":"1.0","type":"link"}