{"product_id":"cyquantifluortm-cell-viability-detection-kit-ua079047","title":"CyQuantiFluorTM Cell Viability Detection Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eDry ice transport. Store protected from light at −20°C. After first use, it can be stored at 4°C; for long-term storage, it is recommended to aliquot as needed and keep at −20°C to avoid repeated freeze-thaw cycles. Refer to the reagent vial label for the expiration date.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003eYouai Bio's CyQuantiFluor™ Cell Viability Assay Kit includes a DNA‑specific binding dye and a background‑quenching dye. The DNA‑specific binding dye exhibits only weak fluorescence in the absence of DNA; however, once it crosses the live cell membrane into the cell and specifically binds to intracellular DNA—primarily within the nucleus—it emits much stronger fluorescence. The fluorescence intensity is proportional to the amount of bound DNA, allowing direct quantification of live cells. The background‑quenching dye cannot penetrate the live cell membrane; instead, it quenches fluorescence arising from DNA bound by the dye but originating outside live cells, such as DNA released from dead cells with ruptured membranes, thereby ensuring that only fluorescence from the dye bound to DNA within live cells is detected. The CyQuantiFluor™ Cell Viability Assay measures the DNA content of cells with intact membranes directly, independent of cellular metabolic status, enabling more accurate counting of live cells. Moreover, its specific binding to DNA further enhances the precision of the results.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe CyQuantiFluor™ Cell Viability Assay Kit is a homogeneous reagent that requires only a single addition for detection, thereby reducing experimental errors that may arise from multiple additions. This reagent features a high signal-to-noise ratio, excellent reproducibility, and superior stability. It can quantify the number of viable cells in both cell proliferation and cytotoxicity assays, making it particularly well-suited for tumor cell proliferation studies and high-throughput screening of compounds.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eCyQuantiFluor\u003c\/span\u003e\u003csup\u003e\u003cspan\u003eTM\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eThe components and specifications of the cell viability assay kit are as follows. Among them,\u003c\/span\u003e\u003cspan\u003efor the\u003c\/span\u003e\u003cspan\u003e96-\u003c\/span\u003e\u003cspan\u003ewell plate and the\u003c\/span\u003e\u003cspan\u003e384-\u003c\/span\u003e\u003cspan\u003ewell plate, the number of detection reactions is calculated based on a medium volume of\u003c\/span\u003e\u003cspan\u003e100 μL\u003c\/span\u003e\u003cspan\u003eper well and\u003c\/span\u003e\u003cspan\u003e, with the test reagent added at\u003c\/span\u003e\u003cspan\u003e100 μL\u003c\/span\u003e\u003cspan\u003eand\u003c\/span\u003e\u003cspan\u003e25 μL\u003c\/span\u003e\u003cspan\u003eper well, respectively.\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eSpecifications\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eComponents and Specifications\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eAssay\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e96-\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eNumber of Wells in 96-Well Plate\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eAssay\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e384-\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eNumber of Wells in 384-Well Plate\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e250 assays\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDNA\u003c\/span\u003e\u003cspan\u003ebinding dye:\u003c\/span\u003e\u003cspan\u003e0.1 mL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003ebackground-quenching dye:\u003c\/span\u003e\u003cspan\u003e0.5 mL\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e250\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1,000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1,250 assays\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDNA\u003c\/span\u003e\u003cspan\u003ebinding dye:\u003c\/span\u003e\u003cspan\u003e0.5 mL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003ebackground-quenching dye:\u003c\/span\u003e\u003cspan\u003e2.5 mL\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1,250\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5,000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e12,500 assays\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDNA\u003c\/span\u003e\u003cspan\u003ebinding dye:\u003c\/span\u003e\u003cspan\u003e5 mL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003ebackground-quenching dye:\u003c\/span\u003e\u003cspan\u003e25 mL\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e12,500\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50,000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eApplicable under most experimental conditions\u003c\/span\u003e\u003cspan\u003eCyQuantiFluor\u003c\/span\u003e\u003csup\u003e\u003cspan\u003eTM\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003eIt is not recommended to directly compare the absolute readings of samples using the cell viability assay kit. It is advised to set the same reference on each experimental plate—for example, in tumor cell proliferation assays, use the same toxic reference compound and solvent carrier control. Normalize the sample readings against those of the toxic reference compound and solvent carrier control before data processing and comparison.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eThe following uses as an example\u003c\/span\u003e\u003cspan\u003e96\u003c\/span\u003e\u003cspan\u003ewell cell culture plates for\u003c\/span\u003e\u003cspan\u003edetection.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan style=\"margin-right: 8px;\"\u003e1)\u003c\/span\u003e\u003cspan\u003eOn a\u003c\/span\u003e\u003cspan\u003e96-\u003c\/span\u003e\u003cspan\u003eA transparent black-bottomed 96-well cell culture plate is seeded with experimental cells at an appropriate density.\u003c\/span\u003e\u003cspan\u003e100\u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan style=\"margin-right: 8px;\"\u003e2)\u003c\/span\u003e\u003cspan\u003ePerform cell treatment according to experimental requirements; for example,\u003c\/span\u003e\u003cspan\u003ein tumor cell proliferation experiments, add the corresponding test compound and incubate for a certain period.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan style=\"margin-right: 8px;\"\u003e3)\u003c\/span\u003e\u003cspan\u003eAfter cell treatment,\u003c\/span\u003e\u003cspan\u003etake out\u003c\/span\u003e\u003cspan\u003eCyQuantiFluor\u003c\/span\u003e\u003csup\u003e\u003cspan\u003eTM\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ecell viability assay kit\u003c\/span\u003e\u003cspan\u003eand perform\u003c\/span\u003e\u003cspan\u003ecell viability\u003c\/span\u003e\u003cspan\u003edetection\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan style=\"margin-right: 8px;\"\u003e4)\u003c\/span\u003e\u003cspan\u003e2x CyQuantiFluor\u003c\/span\u003e\u003csup\u003e\u003cspan\u003eTM\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ePrepare the detection reagent: you can use\u003c\/span\u003e\u003cspan\u003eHBSS (Hank's buffered saline solution), PBS,\u003c\/span\u003e\u003cspan\u003ePreparation of culture media and other solutions, with or without serum\u003c\/span\u003e\u003cspan\u003e2x\u003c\/span\u003e\u003cspan\u003eTest reagent. Dispense the above solution as needed, then add\u003c\/span\u003e\u003cspan\u003e250x\u003c\/span\u003e\u003cspan\u003eof\/'s\u003c\/span\u003e\u003cspan\u003eDNA\u003c\/span\u003e\u003cspan\u003eCombining dyes and\u003c\/span\u003e\u003cspan\u003e50x\u003c\/span\u003e\u003cspan\u003eYou can simply use a background‑blocking dye, for example, to test a piece.\u003c\/span\u003e\u003cspan\u003e96-\u003c\/span\u003e\u003cspan\u003eThe breadboard can be provided as needed.\u003c\/span\u003e\u003cspan\u003ePreparation\u003c\/span\u003e\u003cspan\u003e12 mL\u003c\/span\u003e\u003cspan\u003eof\/'s\u003c\/span\u003e\u003cspan\u003e2x\u003c\/span\u003e\u003cspan\u003eTesting reagents can be directed to\u003c\/span\u003e\u003cspan\u003e11.7 mL\u003c\/span\u003e\u003cspan\u003eof\/'s\u003c\/span\u003e\u003cspan\u003eHank's Balanced Salt Solution\u003c\/span\u003e\u003cspan\u003eAdd to\u003c\/span\u003e\u003cspan\u003eforty-eight\u003c\/span\u003e\u003cspan\u003emu\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003cspan\u003eof\/'s\u003c\/span\u003e\u003cspan\u003eDNA\u003c\/span\u003e\u003cspan\u003eCombination dye\u003c\/span\u003e\u003cspan\u003eand\u003c\/span\u003e\u003cspan\u003e240\u003c\/span\u003e\u003cspan\u003emu\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003cspan\u003eof\/'s\u003c\/span\u003e\u003cspan\u003eBackground-suppressing dye\u003c\/span\u003e\u003cspan\u003eThat's all. Shake to mix.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan style=\"margin-right: 8px;\"\u003e5)\u003c\/span\u003e\u003cspan\u003eTo each well containing\u003c\/span\u003e\u003cspan\u003e100\u003c\/span\u003e\u003cspan\u003emu\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003cspan\u003eThe culture medium's\u003c\/span\u003e\u003cspan\u003e96-\u003c\/span\u003e\u003cspan\u003eConfucius\u003c\/span\u003e\u003cspan\u003eAdd to the experimental cell plate\u003c\/span\u003e\u003cspan\u003e100\u003c\/span\u003e\u003cspan\u003emu\u003c\/span\u003e\u003cspan\u003eL 2x\u003c\/span\u003e\u003cspan\u003eDetection reagents. If each well contains a different volume of culture medium, it should be maintained…\u003c\/span\u003e\u003cspan\u003eThe volume ratio of culture medium to added reagent per well is\u003c\/span\u003e\u003cspan\u003e1 : 1\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan style=\"margin-right: 8px;\"\u003e6)\u003c\/span\u003e\u003cspan\u003e37\u003c\/span\u003e\u003cspan\u003e℃\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e5% CO\u003c\/span\u003e\u003csub\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/sub\u003e\u003cspan\u003eIncubation in an incubator. The test reagent can be added.\u003c\/span\u003e\u003cspan\u003e0.5 hour - 6 hours\u003c\/span\u003e\u003cspan\u003ePlate reading can generally be done in\u003c\/span\u003e\u003cspan\u003e1 hour\u003c\/span\u003e\u003cspan\u003e. The optimal reading time depends on the specific cells and experimental conditions and can be optimized through preliminary experiments. Fluorescence signal intensity typically reaches its peak within\u003c\/span\u003e\u003cspan\u003e30–60 minutes\u003c\/span\u003e\u003cspan\u003eand remains essentially stable for up to\u003c\/span\u003e\u003cspan\u003e6 hours\u003c\/span\u003e\u003cspan\u003e. Note that if the test cells are suspended, ensure they have settled at the bottom of the assay plate before reading; if necessary, gently centrifuge the plate to bring the suspended cells to the bottom.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan style=\"margin-right: 8px;\"\u003e7)\u003c\/span\u003e\u003cspan\u003eUse a\u003c\/span\u003e\u003cspan\u003eFITC\u003c\/span\u003e\u003cspan\u003efilter\u003c\/span\u003e\u003cspan\u003e(Em 490nm\/Ex 520nm)\u003c\/span\u003e\u003cspan\u003eor a similar filter to read the fluorescence signal from the\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003ebottom\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eGuidelines\u003c\/h4\u003e\u003cdiv\u003e1) Mixing different batches is not recommended.\u003cbr\u003e2) Without rigorous validation, it is not recommended to alter the volume of the test reagent.\u003cbr\u003e3) For research use only.\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"250tests","offer_id":42989602111563,"sku":"UA079047-250tests","price":175.0,"currency_code":"USD","in_stock":true},{"title":"1250tests","offer_id":42989602144331,"sku":"UA079047-1250tests","price":545.0,"currency_code":"USD","in_stock":true},{"title":"12500tests","offer_id":42989602177099,"sku":"UA079047-12500tests","price":3750.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_df9ea4bd-3e4d-4dc5-b180-934171b0c69c.png?v=1785916847","url":"https:\/\/www.antbioinc.com\/products\/cyquantifluortm-cell-viability-detection-kit-ua079047","provider":"AntBio","version":"1.0","type":"link"}