{"product_id":"cell-recycling-solution-abs90549","title":"Cell Recovery Solution","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1. Reagent preparation steps:\u003c\/strong\u003e\u003cbr\u003eCell working solution (1X): Cell recovery solution (10X) was diluted into cell working solution (1X) using 4℃ PBS (1X).\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e2. Hydrogel dissolution step:\u003c\/strong\u003e\u003cbr\u003eAll steps were performed in a sterile operating bench, and the operating steps were as follows:\u003cbr\u003e1. After the 3D cell culture is formed, carefully aspirate the culture medium to remove it and wash it with PBS (1X). The PBS was removed by pipetting, and 1 mL of pre-cooled cell working solution (1X) was added to cover the droplets and reacted at room temperature for 5 min.\u003cbr\u003e2. Gently blow with a pipette until the hydrogel is completely dissolved.\u003cbr\u003e3. The solution containing the cell spheres was drawn into a centrifuge tube, centrifuged at 250g for 5 minutes, the supernatant liquid was removed, and the pelleted cell spheres were collected for subsequent experimental analysis.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe cell recovery solution can recover cells cultured in a hydrogel for downstream protein analysis. This solution does not contain any enzyme components, can dissolve the hydrogel without affecting cell activity, does not require enzyme digestion or long-term high-temperature incubation, and can quickly and efficiently recover organoids\/cells from the hydrogel system within 5 minutes.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at 2-8°C with a shelf life of 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe hydrogel was dissolved and the cells were recovered.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"25mL","offer_id":43224150736971,"sku":"abs90549-25mL","price":262.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.antbioinc.com\/products\/cell-recycling-solution-abs90549","provider":"AntBio","version":"1.0","type":"link"}