{"product_id":"cd3ecd3g-heterodimer-fc-his-tagfc-flag-tag-protein-mouse-ua016047","title":"CD3E\u0026CD3G Heterodimer, Fc, His Tag\u0026Fc, Flag Tag Protein, Mouse","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eMouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eCD3E\u0026amp;CD3G Heterodimer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAccession\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eP22646(CD3E)、P11942(CD3G)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAmino Acid Sequence\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eAsp 23 - Asp 108 (CD3E) with hIgG1 Fc Tag at the C-Terminus, followed by His Tag \u003cbr\u003eGln 23 - Ser 116 (CD3G) with hIgG1 Fc Tag at the C-Terminus, followed by Flag Tag\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHEK293\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e55-70kDa (Reducing) \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e＞90% by SDS-PAGE \u0026amp; HPLC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHis Tag, Human IgG1 Fc\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLyophilized powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003ePBS, pH7.4, 5% trehalose\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReconstitution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003eReconstitute at 0.1-1 mg\/ml according to the size in ultrapure water after rapid centrifugation.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.\u003cbr\u003e· 3 months, -20 to -80℃ under sterile conditions after reconstitution.\u003cbr\u003e· 1 week, 2 to 8℃ under sterile conditions after reconstitution.\u003cbr\u003e· Please avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e1. Sun, Z. J., et al. (2001). Mechanisms contributing to T cell receptor signaling and assembly revealed by the solution structure of a heterodimeric complex of the T cell receptor ζ and ε subunits. Nature Immunology, 2(3), 245-251.\u003cbr\u003e2. Kuhns, M. S., Davis, M. M., \u0026amp; Garcia, K. C. (2006). Deconstructing the form and function of the TCR\/CD3 complex. Immunity, 24(2), 133-139.\u003cbr\u003e3. Call, M. E., Pyrdol, J., Wiedmann, M., \u0026amp; Wucherpfennig, K. W. (2002). The organizing principle in the formation of the T cell receptor-CD3 complex. Cell, 111(7), 967-979.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003eCD3ε and CD3γ are two essential and homologous subunits of the T cell receptor (TCR) complex. They are type I transmembrane glycoproteins belonging to the immunoglobulin superfamily. Unlike the variable TCRαβ or TCRγδ chains that recognize antigens, CD3ε and CD3γ, along with CD3δ and the ζ-chain homodimer, form the invariant CD3 signaling module.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThese subunits exist primarily as a CD3εγ heterodimer on the cell surface. Their primary function is to mediate signal transduction from the engaged TCR to the T cell interior. Each subunit contains three immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic tails. Upon TCR-pMHC engagement, these ITAMs are phosphorylated by Src-family kinases, initiating a downstream cascade that leads to T cell activation, proliferation, and differentiation.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eStructurally, the CD3εγ heterodimer plays a critical role in the assembly, surface expression, and conformational stability of the TCR complex. Their extracellular immunoglobulin domains interact with the TCRαβ constant regions, while their unique transmembrane charged residues are crucial for interactions within the complete TCR-CD3 assembly. CD3γ is particularly notable for having a conserved di-leucine-based motif in its cytoplasmic tail that can mediate TCR-CD3 complex internalization and downregulation, a key mechanism for modulating T cell responses. Defects in CD3γ are a known cause of severe combined immunodeficiency (SCID). The CD3εγ pair is also a stable surface marker for T cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"25μg","offer_id":43093872836683,"sku":"UA016047-25μg","price":215.0,"currency_code":"USD","in_stock":true},{"title":"100μg","offer_id":43093872869451,"sku":"UA016047-100μg","price":430.0,"currency_code":"USD","in_stock":true},{"title":"500μg","offer_id":43093872902219,"sku":"UA016047-500μg","price":1205.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/0b464751937047e99f3e8b47c1e7d842.jpg?v=1788199339","url":"https:\/\/www.antbioinc.com\/products\/cd3ecd3g-heterodimer-fc-his-tagfc-flag-tag-protein-mouse-ua016047","provider":"AntBio","version":"1.0","type":"link"}