{"product_id":"cd3ecd3d-heterodimer-fc-his-tagfc-flag-tag-protein-cynomolgus-ua016156","title":"CD3E\u0026CD3D Heterodimer, Fc, His Tag\u0026Fc, Flag Tag Protein, Cynomolgus","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eCD3 epsilon\u0026amp;CD3 delta, CD3E \u0026amp; CD3D\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAccession\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eQ95LI5-1(CD3E)、Q95LI8-1 (CD3D)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAmino Acid Sequence\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eGln 22 - Asp 117 (CD3E) with hIgG1 Fc Tag at the C-Terminus, followed by polyhistidine tag; Phe 22 - Ala 105 (CD3D) with hIgG1 Fc Tag at the C-Terminus, followed by Flag tag\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHEK293\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e40-55kDa\u0026amp;55-70kDa (Reducing)  \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e＞95% by SDS-PAGE \u0026amp; HPLC \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLyophilized Powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003ePBS, pH7.4, 5% trehalose\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReconstitution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eReconstitute at 0.1-1 mg\/ml according to the size in ultrapure water after rapid centrifugation.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e·\u003c\/span\u003e\u003cspan\u003e 12 months from date of receipt, lyophilized powder stored at -20 to -80\u003c\/span\u003e\u003cspan\u003e℃\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e·\u003c\/span\u003e\u003cspan\u003e 3 months, -20 to -80\u003c\/span\u003e\u003cspan\u003e℃\u003c\/span\u003e\u003cspan\u003e under sterile conditions after reconstitution.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e·\u003c\/span\u003e\u003cspan\u003e 1 week, 2 to 8\u003c\/span\u003e\u003cspan\u003e℃\u003c\/span\u003e\u003cspan\u003e under sterile conditions after reconstitution.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e·\u003c\/span\u003e\u003cspan\u003e Please avoid repeated freeze-thaw cycles.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e1. Wang, Z., et al. (2004). Expression and characterization of recombinant soluble monkey CD3 molecules: mapping the FN18 polymorphic epitope. Molecular Immunology, 40(16), 1179-1188. \u003cbr\u003e2. Uda, A., et al. (2003). Identification of an amino acid responsible for the CD3 polymorphism in cynomolgus monkeys (Macaca fascicularis). Journal of Medical Primatology, 32(2), 105-110. \u003cbr\u003e3. Uda, A., et al. (2004). CD3 polymorphism in cynomolgus monkeys (Macaca fascicularis). Journal of Medical Primatology, 33(1), 34-40.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003eCynomolgus macaque (Macaca \u003c\/span\u003e\u003cspan\u003efascicularis\u003c\/span\u003e\u003cspan\u003e) CD3E (CD3 epsilon chain) and CD3D (CD3 delta chain) are two key subunits of the T cell receptor (TCR)-CD3 complex, both of which are single-pass type I membrane glycoproteins. These two subunits form a CD3εδ heterodimer on the cell surface, serving as one of the core signal-transducing components of the TCR-CD3 complex.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe ectodomains of cynomolgus CD3E and CD3D correspond to Gln22-Asp117 of CD3E and Phe22-Ala105 of CD3D, respectively. The \u003c\/span\u003e\u003cspan\u003eUniProt\u003c\/span\u003e\u003cspan\u003e accession numbers are Q95LI5 for CD3E and Q95LI8 for CD3D. Due to glycosylation, recombinant CD3E\u0026amp;CD3D heterodimers typically exhibit higher apparent molecular weights on SDS-PAGE than their calculated values\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eCD3E and CD3D, together with CD3G and CD3Z (CD247), constitute the signaling module of the TCR-CD3 complex. When antigen-presenting cells (APCs) activate the T cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains (CD3D, CD3E, CD3G, and CD3Z). The immunoreceptor tyrosine-based activation motifs (ITAMs) within the cytoplasmic tails of CD3 subunits become phosphorylated, creating docking sites for the protein kinase ZAP-70 and initiating downstream signaling cascades\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"25μg","offer_id":42939117043787,"sku":"UA016156-25μg","price":200.0,"currency_code":"USD","in_stock":true},{"title":"100μg","offer_id":42939117076555,"sku":"UA016156-100μg","price":585.0,"currency_code":"USD","in_stock":true},{"title":"500μg","offer_id":42939117109323,"sku":"UA016156-500μg","price":1750.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_66dcf9db-6105-41da-b8fa-15bbf576363f.png?v=1784822409","url":"https:\/\/www.antbioinc.com\/products\/cd3ecd3d-heterodimer-fc-his-tagfc-flag-tag-protein-cynomolgus-ua016156","provider":"AntBio","version":"1.0","type":"link"}