{"product_id":"cd3-epsilon-fchis-tag-protein-human-ua016209","title":"CD3 epsilon Fc\u0026His Tag Protein, Human","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eCD3 epsilon, CD3E\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAccession\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eP07766-1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAmino Acid Sequence\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eAsp 23 - Asp 126 (CD3E) with hIgG1 Fc Tag at the C-Terminus, followed by polyhistidine tag\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHEK293\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e40-55kDa (Reducing)  \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e＞95% by SDS-PAGE \u0026amp; HPLC \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman IgG1 Fc\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLyophilized Powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003ePBS, pH7.4, 5% trehalose\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReconstitution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eReconstitute at 0.1-1 mg\/ml according to the size in ultrapure water after rapid centrifugation.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e·\u003c\/span\u003e\u003cspan\u003e 12 months from date of receipt, lyophilized powder stored at -20 to -80\u003c\/span\u003e\u003cspan\u003e℃\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e·\u003c\/span\u003e\u003cspan\u003e 3 months, -20 to -80\u003c\/span\u003e\u003cspan\u003e℃\u003c\/span\u003e\u003cspan\u003e under sterile conditions after reconstitution.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e·\u003c\/span\u003e\u003cspan\u003e 1 week, 2 to 8\u003c\/span\u003e\u003cspan\u003e℃\u003c\/span\u003e\u003cspan\u003e under sterile conditions after reconstitution.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e·\u003c\/span\u003e\u003cspan\u003e Please avoid repeated freeze-thaw cycles.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e1. Severe combined immunodeficiency caused by deficiency in either the CD3 epsilon or the CD3 gamma subunit of the T-cell antigen receptor complex. Journal of Clinical Investigation, 114(10), 1512-1517.\u003cbr\u003e2. Structural basis of assembly of the human T cell receptor-CD3 complex. Nature, 585(7823), 116-121.\u003cbr\u003e3. The TCR-CD3 complex: a 3:1 stoichiometry of signaling subunits. Trends in Immunology, 44(5), 345-358.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003eThe CD3E (CD3 epsilon subunit of T-cell receptor complex) gene encodes the CD3ε chain of the T-cell surface glycoprotein, an essential component of the T cell receptor (TCR)-CD3 complex. The gene is located on human chromosome 11q23.3, and the CD3ε protein is a single-pass type I transmembrane protein belonging to the immunoglobulin (Ig) superfamily.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eStructure and Function: Human CD3ε protein consists of an extracellular domain (104 amino acids), a transmembrane domain (26 amino acids), and a cytoplasmic domain (55 amino acids). The extracellular region contains an immunoglobulin-like domain, and the cytoplasmic tail contains an immunoreceptor tyrosine-based activation motif (ITAM) domain. CD3ε, together with CD3γ, CD3δ, and CD3ζ, forms the signaling module of the TCR-CD3 complex. Upon TCR engagement by antigen-presenting cells, the ITAM motifs of CD3 chains become phosphorylated, initiating downstream signaling cascades that convert antigen recognition into intracellular activation signals.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"25μg","offer_id":43089700388939,"sku":"UA016209-25μg","price":235.0,"currency_code":"USD","in_stock":true},{"title":"100μg","offer_id":43089700421707,"sku":"UA016209-100μg","price":470.0,"currency_code":"USD","in_stock":true},{"title":"500μg","offer_id":43089700454475,"sku":"UA016209-500μg","price":1495.0,"currency_code":"USD","in_stock":true},{"title":"1mg","offer_id":43089700487243,"sku":"UA016209-1mg","price":2240.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/afc2a4131f4045949a536b107afa8924.png?v=1788109398","url":"https:\/\/www.antbioinc.com\/products\/cd3-epsilon-fchis-tag-protein-human-ua016209","provider":"AntBio","version":"1.0","type":"link"}