{"product_id":"cd11b-nanobeads-human-and-mouseruo-s0k0007","title":"CD11b NanoBeads, human and mouse（RUO）","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eDextran-coated iron oxide nanomagnetic beads conjugated with anti-human and anti-mouse CD11b monoclonal antibodies, in a PBS buffer system containing BSA and Poloxamer 188.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003e1. For human whole blood or mouse bone marrow cells: Add 20 μL of nanobeads per 10⁷ cells, allowing for up to 100 separations. 2. For human PBMCs: Add 6 μL of nanobeads per 10⁷ cells, allowing for up to 333 separations. 3. For mouse splenocytes: Add 2 μL of nanobeads per 10⁷ cells, allowing for up to 1,000 separations.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eAdopt\u003c\/span\u003e\u003cspan style=\"color:#404040\"\u003eCD11b NanoBeads, human and mouse\u003c\/span\u003e\u003cspan\u003eMagnetic labeling of CD11b⁺ cells. Subsequently, the cell suspension is loaded into a separation column placed within the sorter's magnetic field. The magnetically labeled CD11b⁺ cells remain retained in the column, while the unlabeled cells elute; this fraction constitutes the negative fraction after removal of CD11b⁺ cells. After removing the separation column from the magnetic field, the magnetically captured CD11b⁺ cells can be eluted, yielding the positive sorted fraction.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman, Mouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003ePositive Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8 °C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eCD11b NanoBeads, human and mouse are designed to isolate human and murine cells based on the expression of the CD11b antigen. In humans, CD11b is highly expressed on myeloid cells and weakly expressed on NK cells and some activated lymphocytes. In mice, the CD11b antigen is expressed on monocytes\/macrophages, with lower levels observed on granulocytes, NK cells, CD5+ B1 cells, and certain subsets of dendritic cells. This CD11b antibody (Mac-1 α chain; integrin αM chain) binds to the 170 kDa αM subunit within the CD11b\/CD18 heterodimer (Mac-1, αMβ2 integrin). It functions as a receptor for complement components (C3bi), fibrinogen, or coagulation factor X.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1ml","offer_id":43030517383243,"sku":"S0K0007-1ml","price":0.1,"currency_code":"USD","in_stock":true},{"title":"2ml","offer_id":43030517416011,"sku":"S0K0007-2ml","price":0.1,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/090e7d07900c4e018316021794bf0593.jpg?v=1786510948","url":"https:\/\/www.antbioinc.com\/products\/cd11b-nanobeads-human-and-mouseruo-s0k0007","provider":"AntBio","version":"1.0","type":"link"}