{"product_id":"cd117-nanobeads-mouse-ruo-s0k0031","title":"CD117 Nanobeads, mouse (RUO)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eDextran-coated iron oxide nanomagnetic beads coupled with an anti-mouse CD117 monoclonal antibody are stored in a PBS buffer system containing BSA, Poloxamer 188, and NaN.\u003c\/span\u003e\u003csub\u003e\u003cspan\u003e3\u003c\/span\u003e\u003c\/sub\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e1 mL magnetic beads: suitable for 1 × 10\u003csup\u003e\u003cspan\u003e9\u003c\/span\u003e\u003c\/sup\u003e\u003c\/span\u003e\u003cspan\u003eTotal cell count: up to 100 sorting runs can be performed.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2 mL magnetic beads: suitable for 2 × 10\u003csup\u003e\u003cspan\u003e9\u003c\/span\u003e\u003c\/sup\u003e\u003c\/span\u003e\u003cspan\u003eTotal cell count: up to 200 sorting runs can be performed.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eFirst, CD117-positive cells are magnetically labeled using CD117 Nanobeads, mouse. After labeling, the cell suspension is loaded into a separation column placed within the sorter's magnetic field. The magnetically labeled CD117⁺ cells are retained in the column, while the unlabeled cells flow through. At this point, the cells that elute from the column constitute the negative fraction after removal of CD117⁺ cells. Once the column is removed from the magnetic field, the CD117⁺ cells captured by the magnetic beads are eluted, yielding the positive fraction.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eApplications in cell sorting\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eMouse cells enriched for or depleted of CD117+ cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eMouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003ePositive Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8°C; avoid freezing.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan\u003eMouse CD117, often also referred to as c-Kit or the stem cell factor receptor (SCFR), is a crucial type III receptor tyrosine kinase. It is expressed on most hematopoietic progenitor cells, including multipotent hematopoietic stem cells as well as differentiated myeloid, erythroid, and lymphoid progenitors. It has been reported that, in addition to their hematopoietic differentiation potential, CD117-positive stem cells in mouse bone marrow can also differentiate into smooth muscle cells, muscle cells, and endothelial cells in vivo. CD117 is also expressed on certain mature hematopoietic cells, such as mast cells.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003eNote\u003c\/span\u003e\u003cspan\u003e: This kit does not include the sorting buffer; it must be provided separately. The corresponding Stater product number is S0D3018.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eTaking S\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eSeparation Column\u003c\/span\u003e\u003cspan\u003eas an example\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse:collapse;width:auto;border:1px solid #000000\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eSteps\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eOperating Procedure\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eDosage \/ Time\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"7\" style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eMagnetic Labeling\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTake a mouse tissue sample containing CD117⁺\u003c\/span\u003e\u003cspan\u003ecells, grind it on a 40 μm sterile filter to prepare a single-cell suspension; after red blood cell lysis, perform live cell counting.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eIt is recommended to count cells after staining with AO\/PI.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e—\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCentrifuge the cell suspension at 400×g for 7 minutes, and completely discard the supernatant.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e400×g, 7 min\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eResuspend every 1 × 10⁷ cells in 90 μL of buffer.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eAdjust the volume of buffer proportionally according to the sample volume; (e.g., resuspend 2 × 10⁷ cells in 180 μL of sorting buffer).\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eResuspend the cells.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 10 μL per 1 × 10⁷ cells.\u003c\/span\u003e\u003cspan style=\"color:#404040\"\u003eCD117 Nanobeads, mouse,\u003c\/span\u003e\u003cspan\u003eAnd mix the cell suspension thoroughly, avoiding excessive bubble formation during mixing.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eWhen the cell number increases, increase the volume of all reagents proportionally; (e.g., for 2 × 10⁷ cells, add 20 μL of sorting magnetic beads.)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eFor 1 × 10⁷ cells, add 10 μL.\u003c\/span\u003e\u003cspan style=\"color:#404040\"\u003eCD117 Nanobeads, mouse\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eMix thoroughly and incubate in the dark at 2–8°C for 15 minutes.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate for 15 minutes.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e6\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 1 mL of buffer per 1 × 10⁷ cells to wash, centrifuge at 400 × g for 7 minutes, and completely discard the supernatant.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 1 mL of buffer for 1 × 10⁷ cells.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eResuspend the cells in 500 μL of sorting buffer.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003e500 μL of buffer can resuspend up to 1 × 10⁸ cells.\u003c\/span\u003e\u003cspan\u003eCells\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eResuspend the cells.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"5\" style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eMagnetic separation.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003ePlace the S-shaped separation column into the magnetic field of the corresponding magnetic separator, and rinse the column with 500 μL of buffer.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eRinse the separation column.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e9\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eLoad the cell suspension onto the separation column and collect the flow-through (unlabeled cells).\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e—\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eWash the column with buffer three times, each time using 500 μL; add the next wash only after the liquid in the column has completely drained.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3 × 500 μL of buffer.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e11\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCombine all flow-through fractions as the negative fraction; remove the separation column from the magnetic field and place it over a collection tube.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e—\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e12\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 1 mL of buffer to the column, then quickly and firmly push down with the provided plunger to elute the magnetically labeled positive cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eFor higher purity, use a new column and repeat the separation once.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCollect the target cells.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eIn L\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eTaking the Separation Column\u003c\/span\u003e\u003cspan\u003eas an example.\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse:collapse;width:auto;border:1px solid #000000\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eSteps\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eOperating Procedure\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eDosage \/ Time\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"7\" style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eMagnetic Labeling\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTake CD117⁺ containing\u003c\/span\u003e\u003cspan\u003eMouse tissue samples are ground on a 40 μm sterile filter to prepare a single-cell suspension; after red blood cell lysis, live cell counting is performed.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eNote: It is recommended to count cells after staining with AO\/PI.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e—\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCentrifuge the cell suspension at 400×g for 7 minutes, and completely discard the supernatant.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e400×g, 7 min\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eResuspend every 1 × 10⁷ cells in 90 μL of buffer.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eAdjust the volume of buffer proportionally according to the sample volume; (e.g., resuspend 2 × 10⁷ cells in 180 μL of sorting buffer).\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eResuspend the cells\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 10 μL per 1 × 10⁷ cells\u003c\/span\u003e\u003cspan style=\"color:#404040\"\u003eCD117 Nanobeads, mouse,\u003c\/span\u003e\u003cspan\u003eand mix the cell suspension thoroughly, avoiding excessive bubble formation during mixing.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eWhen the cell number increases, all reagent volumes increase proportionally; (e.g., for 2×10⁷ cells, add 20 μL of sorting magnetic beads)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eFor 1×10⁷ cells, add 10 μL\u003c\/span\u003e\u003cspan style=\"color:#404040\"\u003eCD117 Nanobeads, mouse\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eMix thoroughly and incubate in the dark at 2–8°C for 15 minutes\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate for 15 min\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e6\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eWash with 1 mL of buffer, centrifuge at 400×g for 7 minutes, and completely discard the supernatant\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eFor 1×10⁷ cells, add 1 mL of buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eResuspend the cells in 1 mL of sorting buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eA maximum of 1×10⁸ cells can be resuspended in 500 μL of buffer\u003c\/span\u003e\u003cspan\u003eCells\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eResuspend cells\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"5\" style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eMagnetic separation\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003ePlace the L-shaped separation column into the magnetic field of the corresponding magnetic separator, and rinse the column with 3 mL of buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eRinse the separation column\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e9\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eLoad the cell suspension onto the separation column and collect the flow-through (unlabeled cells)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e—\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eWash the column with buffer 3 times, 3 mL each time, and add the next wash only after the liquid in the column has completely drained.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3 × 3 mL of buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e11\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCombine all eluates into the negative fraction; remove the separation column from the magnetic field and place it over a collection tube.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e—\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e12\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 5 mL of buffer to the column, then quickly and firmly push down with the provided plunger to elute the magnetically labeled positive cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eIf higher purity is required, use a new column and repeat the separation once.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000;border:1px solid #CCCCCC;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCollect the target cells.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1 mL","offer_id":67434522935371,"sku":"S0K0031-1 mL","price":550.0,"currency_code":"USD","in_stock":true},{"title":"2 mL","offer_id":67434522968139,"sku":"S0K0031-2 mL","price":920.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_09cd98fb-07c1-4910-9a35-04cb7917b389.png?v=1794250813","url":"https:\/\/www.antbioinc.com\/products\/cd117-nanobeads-mouse-ruo-s0k0031","provider":"AntBio","version":"1.0","type":"link"}