{"product_id":"cck-8-cell-viability-detection-kit-ua079046","title":"CCK-8 Cell Viability Detection Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003eShipped on dry ice. Store protected fromlight at –20 °C. After the first use, the reagent may be stored at 4 °C. For long‑term storage, aliquot and store at –20 °C. The expiration date is printed on the reagent bottle label.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan lang=\"EN-US\"\u003eThe CCK-8 Cell Viability Detection Kit measures cell viability by detecting the metabolic state of cells using WST\u003c\/span\u003e\u003cspan style=\"font-family:宋体;\"\u003e‑\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e8 (2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium monosodium salt). The colorless, water-soluble tetrazolium salt WST\u003c\/span\u003e\u003cspan style=\"font-family:宋体;\"\u003e‑\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e8 is reduced by intracellular dehydrogenases in the presence of the electron carrier 1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;\"\u003e‑\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003eMethoxy PMS to produce a water\u003c\/span\u003e\u003cspan style=\"font-family:宋体;\"\u003e‑\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003esoluble orange\u003c\/span\u003e\u003cspan style=\"font-family:宋体;\"\u003e‑\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003eyellow formazan dye. The amount of formazan is determined by colorimetry and is directly proportional to the number of living cells. CCK\u003c\/span\u003e\u003cspan style=\"font-family:宋体;\"\u003e‑\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e8 Cell Viability Detection Kit is a homogeneous reagent that can be added directly to cells to quantify viable cells in cell proliferation and cytotoxicity assays. The reagent exhibits very low cytotoxicity and permits prolonged incubation. The detection sensitivity of this CCK\u003c\/span\u003e\u003cspan style=\"font-family:宋体;\"\u003e‑\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e8 kit is superior to that of other tetrazolium salts such as MTT, XTT or MTS.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan lang=\"EN-US\"\u003eThe specifications of CCK\u003c\/span\u003e\u003cspan style=\"font-family:宋体;\"\u003e‑\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e8 Cell Viability Detection Kit are shown below. The number of reactions for 96\u003c\/span\u003e\u003cspan style=\"font-family:宋体;\"\u003e‑\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003ewell and 384\u003c\/span\u003e\u003cspan style=\"font-family:宋体;\"\u003e‑\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003ewell plates is calculated based on a culture medium volume of 100\u003c\/span\u003e\u003cspan style=\"font-family:宋体;\"\u003e μ\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003eL and 40\u003c\/span\u003e\u003cspan style=\"font-family:宋体;\"\u003e μ\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003eL per well, respectively, and addition of 10\u003c\/span\u003e\u003cspan style=\"font-family:宋体;\"\u003e μ\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003eL and 4\u003c\/span\u003e\u003cspan style=\"font-family:宋体;\"\u003e μ\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003eL of detection reagent per well, respectively.\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv align=\"center\"\u003e\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:none;width:317.55pt;\" border=\"1\" cellspacing=\"0\" cellpadding=\"0\" width=\"423\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height:18.85pt;\"\u003e\n\u003ctd style=\"border:solid black 1.0pt;height:18.85pt;padding:0cm 5.4pt 0cm 5.4pt;width:112.05pt;\" width=\"149\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:10.0pt;' lang=\"EN-US\"\u003e\u003cstrong\u003eSize\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid black 1.0pt;height:18.85pt;padding:0cm 5.4pt 0cm 5.4pt;width:97.65pt;\" width=\"130\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:10.0pt;' lang=\"EN-US\"\u003e\u003cstrong\u003eNo. of assays for 96\u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:10.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e‑\u003c\/strong\u003e\u003c\/span\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:10.0pt;' lang=\"EN-US\"\u003e\u003cstrong\u003ewell plates\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid black 1.0pt;height:18.85pt;padding:0cm 5.4pt 0cm 5.4pt;width:107.85pt;\" width=\"144\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:10.0pt;' lang=\"EN-US\"\u003e\u003cstrong\u003eNo. of assays for 384\u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:10.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e‑\u003c\/strong\u003e\u003c\/span\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:10.0pt;' lang=\"EN-US\"\u003e\u003cstrong\u003ewell plates\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:15.7pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid black 1.0pt;height:15.7pt;padding:0cm 5.4pt 0cm 5.4pt;width:112.05pt;\" width=\"149\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:10.0pt;' lang=\"EN-US\"\u003e1mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:15.7pt;padding:0cm 5.4pt 0cm 5.4pt;width:97.65pt;\" width=\"130\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:10.0pt;' lang=\"EN-US\"\u003e100 \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:15.7pt;padding:0cm 5.4pt 0cm 5.4pt;width:107.85pt;\" width=\"144\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:10.0pt;' lang=\"EN-US\"\u003e250 \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:15.7pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid black 1.0pt;height:15.7pt;padding:0cm 5.4pt 0cm 5.4pt;width:112.05pt;\" width=\"149\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:10.0pt;' lang=\"EN-US\"\u003e5 mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:15.7pt;padding:0cm 5.4pt 0cm 5.4pt;width:97.65pt;\" width=\"130\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:10.0pt;' lang=\"EN-US\"\u003e500\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:15.7pt;padding:0cm 5.4pt 0cm 5.4pt;width:107.85pt;\" width=\"144\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:10.0pt;' lang=\"EN-US\"\u003e1,250\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:16.8pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid black 1.0pt;height:16.8pt;padding:0cm 5.4pt 0cm 5.4pt;width:112.05pt;\" width=\"149\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:10.0pt;' lang=\"EN-US\"\u003e10 mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:16.8pt;padding:0cm 5.4pt 0cm 5.4pt;width:97.65pt;\" width=\"130\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:10.0pt;' lang=\"EN-US\"\u003e1,000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:16.8pt;padding:0cm 5.4pt 0cm 5.4pt;width:107.85pt;\" width=\"144\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:10.0pt;' lang=\"EN-US\"\u003e2,500\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:16.8pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid black 1.0pt;height:16.8pt;padding:0cm 5.4pt 0cm 5.4pt;width:112.05pt;\" width=\"149\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:10.0pt;' lang=\"EN-US\"\u003e30 mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:16.8pt;padding:0cm 5.4pt 0cm 5.4pt;width:97.65pt;\" width=\"130\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:10.0pt;' lang=\"EN-US\"\u003e3,000 \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:16.8pt;padding:0cm 5.4pt 0cm 5.4pt;width:107.85pt;\" width=\"144\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:10.0pt;' lang=\"EN-US\"\u003e7,500 \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:15.7pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid black 1.0pt;height:15.7pt;padding:0cm 5.4pt 0cm 5.4pt;width:112.05pt;\" width=\"149\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:10.0pt;' lang=\"EN-US\"\u003e100 mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:15.7pt;padding:0cm 5.4pt 0cm 5.4pt;width:97.65pt;\" width=\"130\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:10.0pt;' lang=\"EN-US\"\u003e10,000 \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:15.7pt;padding:0cm 5.4pt 0cm 5.4pt;width:107.85pt;\" width=\"144\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:10.0pt;' lang=\"EN-US\"\u003e25,000 \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003c\/div\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan lang=\"EN-US\"\u003eThe CCK\u003c\/span\u003e\u003cspan style=\"font-family:宋体;\"\u003e‑\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e8 Cell Viability Detection Kit measures the number of viable cells through a WST\u003c\/span\u003e\u003cspan style=\"font-family:宋体;\"\u003e‑\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e8 redox reaction. Redox substances present in the culture medium may affect the reaction and thus the results. A cell\u003c\/span\u003e\u003cspan style=\"font-family:宋体;\"\u003e‑\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003efree medium control can be included to assess the magnitude of this effect, or the experimental values can be corrected by subtracting the cell\u003c\/span\u003e\u003cspan style=\"font-family:宋体;\"\u003e‑\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003efree medium control values. Under most experimental conditions, direct comparison of absolute sample readings is not recommended. It is advisable to include the same reference controls on each plate (e.g., when testing tumor cell proliferation, include the same reference toxic compound and solvent vehicle control). Normalize sample readings to those of the reference toxic compound and solvent vehicle control before data processing and comparison.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan lang=\"EN-US\"\u003eThe procedure below is described for 96\u003c\/span\u003e\u003cspan style=\"font-family:宋体;\"\u003e‑\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003ewell cell culture plates.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan lang=\"EN-US\"\u003e1. Seed the desired cell density in 100\u003c\/span\u003e\u003cspan style=\"font-family:宋体;\"\u003e μ\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003eL of medium per well of a 96\u003c\/span\u003e\u003cspan style=\"font-family:宋体;\"\u003e‑\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003ewell plate with a clear flat bottom.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan lang=\"EN-US\"\u003e2. Treat the cells according to the experimental design (e.g., for tumor cell proliferation assays, add the test compounds and incubate for the required time).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan lang=\"EN-US\"\u003e3. After cell treatment, remove the CCK\u003c\/span\u003e\u003cspan style=\"font-family:宋体;\"\u003e‑\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e8 Cell Viability Detection Kit reagent from storage for cell viability detection.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan lang=\"EN-US\"\u003e4. Add 10\u003c\/span\u003e\u003cspan style=\"font-family:宋体;\"\u003e μ\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003eL of CCK\u003c\/span\u003e\u003cspan style=\"font-family:宋体;\"\u003e‑\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e8 Cell Viability Detection reagent to each well of the 96\u003c\/span\u003e\u003cspan style=\"font-family:宋体;\"\u003e‑\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003ewell plate that contains 100\u003c\/span\u003e\u003cspan style=\"font-family:宋体;\"\u003e μ\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003eL of culture medium. If a different culture volume is used per well, maintain a ratio of culture medium to added reagent of 10\u003c\/span\u003e\u003cspan style=\"font-family:宋体;\"\u003e \u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e:\u003c\/span\u003e\u003cspan style=\"font-family:宋体;\"\u003e \u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e1.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan lang=\"EN-US\"\u003e5.  Incubate the plate at 37\u003c\/span\u003e\u003cspan style=\"font-family:宋体;\"\u003e °\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003eC in a 5\u003c\/span\u003e\u003cspan style=\"font-family:宋体;\"\u003e \u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e% CO\u003c\/span\u003e\u003cspan style=\"font-family:宋体;\"\u003e₂\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e incubator. Read the plate after 1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;\"\u003e–\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e4\u003c\/span\u003e\u003cspan style=\"font-family:宋体;\"\u003e \u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003ehours; the earliest readout is 0.5\u003c\/span\u003e\u003cspan style=\"font-family:宋体;\"\u003e \u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003ehour. The optimal reading time depends on the specific cells and experimental conditions and can be determined by preliminary experiments.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan lang=\"EN-US\"\u003e6.  Measure the absorbance at 450\u003c\/span\u003e\u003cspan style=\"font-family:宋体;\"\u003e \u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003enm. Other filters with wavelengths between 430 and 490\u003c\/span\u003e\u003cspan style=\"font-family:宋体;\"\u003e \u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003enm can also be used, but the 450\u003c\/span\u003e\u003cspan style=\"font-family:宋体;\"\u003e \u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003enm filter provides the highest detection sensitivity.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eGuidelines\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e1. Do not mix different batches of the kit.\u003c\/p\u003e\n\u003cp\u003e2. Do not alter the volume of detection reagent without rigorousvalidation.\u003c\/p\u003e\n\u003cp\u003e3. For research use only.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"1ml","offer_id":43095292870731,"sku":"UA079046-1ml","price":23.0,"currency_code":"USD","in_stock":true},{"title":"5ml","offer_id":43095292903499,"sku":"UA079046-5ml","price":75.0,"currency_code":"USD","in_stock":true},{"title":"10ml","offer_id":43095292936267,"sku":"UA079046-10ml","price":145.0,"currency_code":"USD","in_stock":true},{"title":"30ml","offer_id":43095292969035,"sku":"UA079046-30ml","price":280.0,"currency_code":"USD","in_stock":true},{"title":"100ml","offer_id":43095293001803,"sku":"UA079046-100ml","price":815.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_88526b21-eda9-4917-8a0e-533d241f9b7e.png?v=1788213788","url":"https:\/\/www.antbioinc.com\/products\/cck-8-cell-viability-detection-kit-ua079046","provider":"AntBio","version":"1.0","type":"link"}