{"product_id":"cathepsin-s-his-tag-protein-human-ua011220","title":"Cathepsin S His Tag Protein, Human","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eCTSS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAccession\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eP25774\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAmino Acid Sequence\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eGln17-Ile331 with His Tag at the C-Terminus\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHEK293\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e35-40kDa (Reducing)\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e＞90% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHis Tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLyophilized powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003ePBS, PH7.4, 5% trehalose\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReconstitution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003eReconstitute at 0.1-1 mg\/ml according to the size in ultrapure water after rapid centrifugation.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.\u003cbr\u003e· 3 months, -20 to -80℃ under sterile conditions after reconstitution.\u003cbr\u003e· 1 week, 2 to 8℃ under sterile conditions after reconstitution.\u003cbr\u003e· Please avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e1.Taheri Baghmisheh S, Chen CH, Yeh YM, Lin PC, Chen PC, Chan RH, Kang JW, Lee CT, Tsai HJ, Fang YC, Cheung CHA, Chang KY, Chang JY, Chen SH. Cathepsin S regulates antitumor immunity through autophagic degradation of PD-L1 in colorectal cancer cells. Cancer Immunol Immunother. 2025 Aug 12;74(9):287.\u003cbr\u003e2.Geetha D, Skaria T. Cathepsin S: A key drug target and signalling hub in immune system diseases. Int Immunopharmacol. 2025 May 16;155:114622.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eCathepsin S (CTSS) is a member of the lysosomal cysteine protease C1 family. It is synthesized as an inactive precursor of 331 amino acids and activated in lysosomes into a mature protease of 217 amino acids through proteolytic removal of its 99-amino acid propeptide. It is primarily expressed in the lysosomes of antigen-presenting cells, such as dendritic cells, B cells, and macrophages.Its core functions are characterized by unique substrate specificity:1) Key Enzyme in Antigen Presentation: It specifically degrades the invariant chain of MHC class II molecules, an essential step for presenting antigenic peptides to CD4⁺ T cells, and also participates in the direct processing and cross-presentation of exogenous antigens.2) Extracellular Matrix Degradation: As an elastase, it can degrade extracellular matrix components over a broad pH range.It holds significant clinical relevance, as dysregulation of its activity is closely associated with various diseases, including arthritis,atherosclerosis,neurological disorders, and chronic obstructive pulmonary disease. It primarily contributes to pathological processes by regulating inflammatory responses, immune reactions, and tissue remodeling, making it a potential therapeutic target for these conditions.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;' lang=\"EN-US\"\u003e\u003cstrong\u003eAssay protocol\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"line-height:150%;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;line-height:150%;' lang=\"EN-US\"\u003e\u003cstrong\u003ePrinciple:\u003c\/strong\u003e Measured by its ability to cleave the fluorogenic peptide substrate, MCA RPKPVE-Nval-WRK(Dnp)-NH2.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"line-height:150%;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;line-height:150%;' lang=\"EN-US\"\u003e\u003cstrong\u003eMaterials\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003col style=\"padding-left:24px;\"\u003e\n\u003cli\u003e\u003cp style=\"line-height:150%;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;line-height:150%;' lang=\"EN-US\"\u003eAssay Buffer: 50 mM NaOAc, 5 mM DTT, 250 mM NaCl, pH 4.5\u003c\/span\u003e\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp style=\"line-height:150%;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;line-height:150%;' lang=\"EN-US\"\u003eCathepsin S His Tag Protein, Human \u003c\/span\u003e\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp style=\"line-height:150%;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;line-height:150%;' lang=\"EN-US\"\u003eSubstrate: MCA-Arg-Pro-Lys-Pro-Val-Glu-NVAL-Trp-Arg-Lys(DNP) NH2 (ES002)\u003c\/span\u003e\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp style=\"line-height:150%;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;line-height:150%;' lang=\"EN-US\"\u003e96 ELISA Removable Plate, Black, High binding (GENEVER, Catalog # GMO2-96H)\u003c\/span\u003e\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp style=\"line-height:150%;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;line-height:150%;' lang=\"EN-US\"\u003ePlate Reader (PerkinElmer, excitation 320 nm and emission 405 nm)\u003c\/span\u003e\u003c\/p\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp style=\"line-height:150%;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;line-height:150%;' lang=\"EN-US\"\u003e\u003cstrong\u003eProduce\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003col style=\"padding-left:24px;\"\u003e\n\u003cli\u003e\u003cp style=\"line-height:150%;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;line-height:150%;' lang=\"EN-US\"\u003eDilute Cathepsin S protein to 10 µg\/mL in Assay Buffer.\u003c\/span\u003e\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp style=\"line-height:150%;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;line-height:150%;' lang=\"EN-US\"\u003eIncubate at room temperature for 2 hours.\u003c\/span\u003e\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp style=\"line-height:150%;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;line-height:150%;' lang=\"EN-US\"\u003eDilute Cathepsin S protein to 0.25 µg\/mL\u003c\/span\u003e\u003cspan style=\"line-height:150%;\"\u003e、\u003c\/span\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;line-height:150%;' lang=\"EN-US\"\u003e0.125 µg\/mL in Assay Buffer.\u003c\/span\u003e\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp style=\"line-height:150%;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;line-height:150%;' lang=\"EN-US\"\u003eDilute substrate to 80 µM in Assay Buffer.\u003c\/span\u003e\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp style=\"line-height:150%;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;line-height:150%;' lang=\"EN-US\"\u003eLoad 50 µL of the 0.25 µg\/mL\u003c\/span\u003e\u003cspan style=\"line-height:150%;\"\u003e、\u003c\/span\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;line-height:150%;' lang=\"EN-US\"\u003e0.125 µg\/mL Cathepsin S protein in a black well plate and start the reaction by adding 50 µL of 80 µM Substrate. Include a Substrate with Assay Buffer as Blank.\u003c\/span\u003e\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp style=\"line-height:150%;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;line-height:150%;' lang=\"EN-US\"\u003eRead at excitation and emission wavelengths of 320 nm and 405 nm, kinetic mode,60s\/cycle, 5 cycles.\u003c\/span\u003e\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp style=\"line-height:150%;text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;line-height:150%;' lang=\"EN-US\"\u003eCalculate specific activity:\u003c\/span\u003e\u003c\/p\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;\" border=\"0\" cellspacing=\"0\" cellpadding=\"0\" width=\"574\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding:7.5pt 7.5pt 7.5pt 7.5pt;width:89.2pt;\" rowspan=\"2\" width=\"119\"\u003e\u003cp style=\"line-height:150%;margin-bottom:7.5pt;text-align:center;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;line-height:150%;' lang=\"EN-US\"\u003eSpecific Activity (pmol\/min\/µg) =\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border:none;padding:7.5pt 7.5pt 1.5pt 7.5pt;width:341.1pt;\" width=\"455\"\u003e\u003cp style=\"line-height:150%;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;line-height:150%;' lang=\"EN-US\"\u003eAdjusted Fluorescence* (RFU) xConversion Factor** (pmol\/RFU)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border:none;padding:7.5pt 7.5pt 7.5pt 7.5pt;width:341.1pt;\" width=\"455\"\u003e\u003cp style=\"line-height:150%;text-indent:10.5pt;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;line-height:150%;' lang=\"EN-US\"\u003eIncubation time (min) x amount of enzyme (µg)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp style=\"line-height:150%;text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:white;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;line-height:150%;' lang=\"EN-US\"\u003e*Adjusted for Substrate Blank\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"line-height:150%;text-align:left;\" align=\"left\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;line-height:150%;' lang=\"EN-US\"\u003e**Derived using calibration standard MCA-Pro-Leu-OH.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"line-height:150%;text-align:center;\"\u003e \u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"25μg","offer_id":42332764373067,"sku":null,"price":215.0,"currency_code":"USD","in_stock":true},{"title":"100μg","offer_id":42332764405835,"sku":null,"price":615.0,"currency_code":"USD","in_stock":true},{"title":"500μg","offer_id":42332764438603,"sku":null,"price":1095.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_a6f06f7d-f804-4c05-bb2a-846d53c1fc14.png?v=1776073649","url":"https:\/\/www.antbioinc.com\/products\/cathepsin-s-his-tag-protein-human-ua011220","provider":"AntBio","version":"1.0","type":"link"}