{"product_id":"cathepsin-f-his-tag-protein-human-ua011221","title":"Cathepsin F His Tag Protein, Human","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eCathepsin F,CTSF\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAccession\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eQ9UBX1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAmino Acid Sequence\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eAla20-Asp484 with His Tag at the C-Terminus\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHEK293\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e60-75kDa (Reducing)\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e＞95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHis Tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLyophilized powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003ePBS, PH7.4, 5% trehalose\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReconstitution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003eReconstitute at 0.1-1 mg\/ml according to the size in ultrapure water after rapid centrifugation.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.\u003cbr\u003e· 3 months, -20 to -80℃ under sterile conditions after reconstitution.\u003cbr\u003e· 1 week, 2 to 8℃ under sterile conditions after reconstitution.\u003cbr\u003e· Please avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e1.Wang X, Zhu Z. Unraveling the causal relationship and underlying mechanisms between cathepsins on liver cancer: findings from mendelian randomization and bioinformatics analysis. Discov Oncol. 2025 Mar 7;16(1):277.\u003cbr\u003e2.Mohan S, Sampognaro PJ, Argouarch AR, Maynard JC, Welch M, Patwardhan A, Courtney EC, Zhang J, Mason A, Li KH, Huang EJ, Seeley WW, Miller BL, Burlingame A, Jacobson MP, Kao AW. Processing of progranulin into granulins involves multiple lysosomal proteases and is affected in frontotemporal lobar degeneration. Mol Neurodegener.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eCTSF (Cathepsin F) is a cysteine protease primarily localized in lysosomes. Its protein structure contains a typical catalytic domain of cysteine proteases, which specifically cleaves peptide bonds through its active site. Within the cell, it is responsible for degrading abnormal or damaged proteins and participates in antigen processing and presentation, thereby regulating immune responses. Clinically, aberrant expression or dysfunction of CTSF is closely associated with various diseases: its overexpression may promote tumor invasion and metastasis, while in autoimmune diseases, it may lead to immune imbalance due to disrupted antigen processing. Consequently, CTSF has emerged as a potential disease biomarker and therapeutic target.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cstrong\u003eExperimental Methods\u003c\/strong\u003e\u003cbr\u003ePrinciple: To assess the ability of Cathepsin F His Tag Protein, Human to cleave the fluorescent peptide substrate Z-FR-AMC.\u003cbr\u003eMaterials:\u003cbr\u003eProtein activation buffer: 50 mM sodium acetate pH 4.5, 5 mM DTT\u003cbr\u003eAssay buffer: 100 mM Sodium acetate (pH 5.5), 2 mM EDTA, 8 mM DTT\u003cbr\u003eCathepsin F His Tag Protein, Human (UA011221)\u003cbr\u003eSubstrate: Z-FR-AMC (C350145)\u003cbr\u003e96-well ELISA Removable Plate, Black, High binding (GENEVER, Catalog # GMO2-96H)\u003cbr\u003ePlate reader (PerkinElmer, excitation wavelength 360 nm, emission wavelength 460 nm)\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eExperimental Procedure\u003c\/strong\u003e\u003cbr\u003eActivate Cathepsin F His Tag Protein, Human in protein activation buffer at room temperature for 90 minutes before the reaction.\u003cbr\u003eDilute the activated Cathepsin F His Tag Protein, Human in assay buffer to concentrations of 15 µg\/mL, 7.5 µg\/mL, 3.75 µg\/mL, 1.875 µg\/mL, 0.93575 µg\/mL, 0.48 µg\/mL, and 0 µg\/mL.\u003cbr\u003eDilute Z-FR-AMC to 40 µM in assay buffer.\u003cbr\u003eAdd 50 µL of the diluted Cathepsin F His Tag Protein, Human to each well of a black plate, followed by 50 µL of 40 µM substrate, and incubate at 30°C for 40-120 min.\u003cbr\u003eMeasure fluorescence at excitation and emission wavelengths of 360 nm and 460 nm, respectively.\u003cbr\u003eCalculate specific activity:\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:none;margin-left:6.7500pt;margin-right:6.7500pt;width:430.3000pt;\" border=\"0\" cellspacing=\"0\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-bottom:1.0000pt solid windowtext;border-left:1.0000pt solid windowtext;border-right:1.0000pt solid windowtext;border-top:1.0000pt solid windowtext;padding:7.5000pt 7.5000pt 7.5000pt 7.5000pt;width:112.0500pt;\" rowspan=\"2\" width=\"224\" valign=\"center\"\u003e\u003cspan style=\"font-family:Calibri;font-size:10.5000pt;\"\u003eSpecific Activity (pmol\/min\/µg) =\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:1.0000pt solid windowtext;border-left:none;border-right:1.0000pt solid windowtext;border-top:1.0000pt solid windowtext;padding:7.5000pt 7.5000pt 1.5000pt 7.5000pt;width:318.2500pt;\" width=\"636\" valign=\"center\"\u003e\u003cspan style=\"font-family:Calibri;font-size:10.5000pt;\"\u003eAdjusted Fluorescence* (RFU) x Conversion Factor** (pmol\/RFU)\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border-bottom:1.0000pt solid windowtext;border-left:none;border-right:1.0000pt solid windowtext;border-top:none;padding:7.5000pt 7.5000pt 7.5000pt 7.5000pt;width:318.2500pt;\" width=\"636\" valign=\"center\"\u003e\u003cspan style=\"font-family:Calibri;font-size:10.5000pt;\"\u003eIncubation time (min) x amount of enzyme (µg)\u003c\/span\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e*Blank-corrected substrate fluorescence\u003cbr\u003e**Derived using the calibration standard 7-amino-4-Methyl Coumarin\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"25μg","offer_id":42332760080459,"sku":null,"price":250.0,"currency_code":"USD","in_stock":true},{"title":"100μg","offer_id":42332760113227,"sku":null,"price":700.0,"currency_code":"USD","in_stock":true},{"title":"500μg","offer_id":42332760145995,"sku":null,"price":1680.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_35186740-4eb6-4ea1-b740-bd8b54ceedbd.png?v=1780633678","url":"https:\/\/www.antbioinc.com\/products\/cathepsin-f-his-tag-protein-human-ua011221","provider":"AntBio","version":"1.0","type":"link"}