{"product_id":"cathepsin-b-his-tag-protein-human-ua100041","title":"Cathepsin B His tag Protein, Human","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eAPP secretase (APPS), Cathepsin B1, CPSB\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAccession\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eP07858\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAmino Acid Sequence\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eMet1-Ile339, with C-10*His\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHEK293\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003ePredicted MW: 39.5 kDa Observed MW: 43 kDa\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u0026gt;95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eEndotoxin\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u0026lt;1EU\/μg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHis Tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLyophilized powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e0.2M PBS, pH7.4.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReconstitution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003eReconstitute no more than 1 mg\/mL according to the size in deionized water after rapid centrifugation.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cspan\u003e\u003cbr\u003e12 months from date of receipt, -20 to -70 °C as supplied.\u003cbr\u003e6 months, -20 to -70 °C under sterile conditions after reconstitution.\u003cbr\u003e1 week, 2 to 8 °C under sterile conditions after reconstitution.\u003cbr\u003ePlease avoid repeated freeze-thaw cycles.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eCathepsin B belongs to a family of lysosomal cysteine proteases known as the cysteine cathepsins and plays an important role in intracellular proteolysis. In humans, cathepsin B is encoded by the CTSB gene. Cathepsin B is upregulated in certain cancers, in pre-malignant lesions, and in various other pathological conditions. Cathepsin B may enhance the activity of other proteases, including matrix metalloproteinase, urokinase (serine protease urokinase plasminogen activator), and cathepsin D, and thus it has an essential position for the proteolysis of extracellular matrix components, intercellular communication disruption, and reduced protease inhibitor expression. Cells may become carcinogenic when cathepsin B is unregulated. Cathepsin B has been proposed as a potentially effective biomarker for a variety of cancers. Overexpression of cathepsin B is correlated with invasive and metastatic cancers.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003eAssay protocol\u003cbr\u003ePrinciple: Measured by its ability to cleave the fluorogenic peptide substrate, Z-LR-AMC.\u003cbr\u003eMaterials\u003cbr\u003e1.Protein Activation Buffer: 25 mM MES, 5 mM DTT, pH 5.0\u003cbr\u003e2.Assay Buffer: 25 mM MES,0.02% Brij-35, pH 5.0\u003cbr\u003e3.Human Cathepsin B, His tag)\u003cbr\u003e4.Substrate: Z-LR-AMC (ES008) \u003cbr\u003e5.96 ELISA Removable Plate, Black, High binding (GENEVER, Catalog # GMO2-96H)\u003cbr\u003e6.Plate Reader (PerkinElmer, excitation 380 nm and emission 460 nm)\u003cbr\u003eProduce\u003cbr\u003e1.Dilute Cathepsin B to 10 μg\/mL in Activation Buffer.\u003cbr\u003e2.Incubate at room temperature for 15 minutes.\u003cbr\u003e3.Dilute Cathepsin B to 0.4, 0.2 and 0.1 μg\/mL in Assay Buffer.\u003cbr\u003e4.Dilute substrate to 80 μM in Assay Buffer.\u003cbr\u003e5.Load 50 μL of the 0.4, 0.2 and 0.1 μg\/mL Cathepsin B in a black well plate, and start the reaction by adding 50 μL of 80 μM Substrate. Include a Substrate Blank containing 50 μL Assay Buffer and 50 μL of 80 μM Substrate.\u003cbr\u003e6.Read at excitation and emission wavelengths of 380 nm and 460 nm (top read), respectively, in kinetic mode for 5 minutes. Calculate specific activity:\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:none;margin-left:-2.1000pt;\" border=\"0\" cellspacing=\"0\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-bottom:none;border-left:none;border-right:none;border-top:none;padding:7.5000pt 7.5000pt 7.5000pt 7.5000pt;\" rowspan=\"2\" valign=\"center\"\u003e\u003cp style=\"line-height:150%;margin-bottom:7.5000pt;text-align:center;\"\u003e\u003cspan style=\"font-family:'Times New Roman';font-size:12.0000pt;line-height:150%;\"\u003eSpecific Activity (pmol\/min\/µg) =\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:1.0000pt solid rgb(0,0,0);border-left:none;border-right:none;border-top:none;padding:7.5000pt 7.5000pt 1.5000pt 7.5000pt;\" valign=\"center\"\u003e\u003cp style=\"line-height:150%;text-align:center;\"\u003e\u003cspan style=\"font-family:'Times New Roman';font-size:12.0000pt;line-height:150%;\"\u003eAdjusted Vmax* (RFU\/min) x Conversion Factor** (pmol\/RFU)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border-bottom:1.0000pt solid rgb(0,0,0);border-left:none;border-right:none;border-top:none;padding:7.5000pt 7.5000pt 7.5000pt 7.5000pt;\" valign=\"center\"\u003e\u003cp style=\"line-height:150%;text-align:center;\"\u003e\u003cspan style=\"font-family:'Times New Roman';font-size:12.0000pt;line-height:150%;\"\u003eamount of enzyme (µg)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e*Adjusted for Substrate Blank\u003cbr\u003e**Derived using calibration standard 7-amino-4-Methyl Coumarin\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"25μg","offer_id":42332759228491,"sku":null,"price":310.0,"currency_code":"USD","in_stock":true},{"title":"50μg","offer_id":42332759261259,"sku":null,"price":570.0,"currency_code":"USD","in_stock":true},{"title":"100μg","offer_id":42332759294027,"sku":null,"price":910.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_9f42080d-07ce-415b-8ff5-9e44c442f50c.png?v=1776073502","url":"https:\/\/www.antbioinc.com\/products\/cathepsin-b-his-tag-protein-human-ua100041","provider":"AntBio","version":"1.0","type":"link"}