{"product_id":"bcr-flbd-ua086054","title":"Human Single-Cell BCR FL Amplification Kit (BD Platform)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eThis product is mainly applied to the determination of paired full-length VDJ sequences in human single B cells. It is compatible with the BD Rhapsody single-cell sequencing platform and uses optimized targeted amplification primer sequences and the most suitable enzyme reaction system. It can simultaneously effectively amplify and detect heavy and light chain sequence information such as IGHG, IGHM, IGHA, IGKC, and IGLC of human single B cells.\u003cbr\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cfigure class=\"table\"\u003e\u003ctable style='-webkit-tap-highlight-color:transparent;-webkit-text-stroke-width:0px;background-color:rgb(255, 255, 255);border-collapse:collapse;border-spacing:0px;box-sizing:border-box;color:rgb(0, 0, 0);font-family:\"Microsoft YaHei\";font-size:14px;font-style:normal;font-variant-caps:normal;font-variant-ligatures:normal;font-weight:400;height:358px;letter-spacing:normal;margin:0px auto;max-width:100%;orphans:2;text-align:center;text-decoration-color:initial;text-decoration-style:initial;text-decoration-thickness:initial;text-transform:none;white-space:normal;widows:2;width:588.458px;word-spacing:0px;' border=\"1\"\u003e\u003ctbody style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;\"\u003e\n\u003ctr style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:40px;\"\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:40px;padding:0px;text-align:center;width:257.698px;word-break:inherit;\"\u003e\u003cstrong style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;\"\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:40px;padding:0px;text-align:center;width:165.031px;word-break:inherit;\"\u003e\u003cstrong style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;\"\u003eSize\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:40px;padding:0px;text-align:center;width:165.062px;word-break:inherit;\"\u003e\u003cstrong style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;\"\u003eVolume\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;\"\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:257.698px;word-break:inherit;\"\u003eTSO\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:165.031px;word-break:inherit;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:165.062px;word-break:inherit;\"\u003e30uL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;\"\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:257.698px;word-break:inherit;\"\u003e1M MgCl\u003csub style=\"-webkit-tap-highlight-color:transparent;bottom:-0.25em;box-sizing:border-box;font-size:10.5px;line-height:0;position:relative;vertical-align:baseline;\"\u003e2\u003c\/sub\u003e\n\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:165.031px;word-break:inherit;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:165.062px;word-break:inherit;\"\u003e15uL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;\"\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:257.698px;word-break:inherit;\"\u003eHybridization Buffer\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:165.031px;word-break:inherit;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:165.062px;word-break:inherit;\"\u003e1.6mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;\"\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:257.698px;word-break:inherit;\"\u003eExtension Enzyme\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:165.031px;word-break:inherit;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:165.062px;word-break:inherit;\"\u003e70uL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;\"\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:257.698px;word-break:inherit;\"\u003eExtension Buffer\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:165.031px;word-break:inherit;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:165.062px;word-break:inherit;\"\u003e160uL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;\"\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:257.698px;word-break:inherit;\"\u003eExtension Primers\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:165.031px;word-break:inherit;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:165.062px;word-break:inherit;\"\u003e30uL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;\"\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:257.698px;word-break:inherit;\"\u003edNTP\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:165.031px;word-break:inherit;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:165.062px;word-break:inherit;\"\u003e200uL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;\"\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:257.698px;word-break:inherit;\"\u003eNuclease Free Water\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:165.031px;word-break:inherit;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:165.062px;word-break:inherit;\"\u003e1mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;\"\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:257.698px;word-break:inherit;\"\u003ePCR MasterMix\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:165.031px;word-break:inherit;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:165.062px;word-break:inherit;\"\u003e1.2mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;\"\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:257.698px;word-break:inherit;\"\u003eBead RT\/PCR Enhancer\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:165.031px;word-break:inherit;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:165.062px;word-break:inherit;\"\u003e200uL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;\"\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:257.698px;word-break:inherit;\"\u003eVDJ Universal Oligo N1\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:165.031px;word-break:inherit;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:165.062px;word-break:inherit;\"\u003e65uL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;\"\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:257.698px;word-break:inherit;\"\u003eVDJ Universal Oligo N2\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:165.031px;word-break:inherit;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:165.062px;word-break:inherit;\"\u003e30uL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;\"\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:257.698px;word-break:inherit;\"\u003eBCR N1 Primer\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:165.031px;word-break:inherit;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:165.062px;word-break:inherit;\"\u003e16uL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;\"\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:257.698px;word-break:inherit;\"\u003eBCR N2 Primer\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:165.031px;word-break:inherit;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;padding:0px;width:165.062px;word-break:inherit;\"\u003e40uL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:22px;\"\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:10px;padding:0px;width:257.698px;word-break:inherit;\"\u003eElution Buffer\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:10px;padding:0px;width:165.031px;word-break:inherit;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"-webkit-tap-highlight-color:transparent;box-sizing:border-box;height:10px;padding:0px;width:165.062px;word-break:inherit;\"\u003e1.8mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp class=\"ds-markdown-paragraph\" style='-webkit-text-stroke-width:0px;background-color:rgb(255, 255, 255);color:rgb(15, 17, 21);font-family:quote-cjk-patch, Inter, system-ui, -apple-system, BlinkMacSystemFont, \"Segoe UI\", Roboto, Oxygen, Ubuntu, Cantarell, \"Open Sans\", \"Helvetica Neue\", sans-serif;font-size:16px;font-style:normal;font-variant-caps:normal;font-variant-ligatures:normal;font-weight:400;letter-spacing:normal;margin:0px !important 0px 16px;orphans:2;text-align:start;text-decoration-color:initial;text-decoration-style:initial;text-indent:0px;text-transform:none;white-space:normal;widows:2;word-spacing:0px;'\u003e\u003cspan\u003eRefer to the BD Rhapsody Full-Length VDJ Library Construction Method.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"ds-markdown-paragraph\" style='-webkit-text-stroke-width:0px;background-color:rgb(255, 255, 255);color:rgb(15, 17, 21);font-family:quote-cjk-patch, Inter, system-ui, -apple-system, BlinkMacSystemFont, \"Segoe UI\", Roboto, Oxygen, Ubuntu, Cantarell, \"Open Sans\", \"Helvetica Neue\", sans-serif;font-size:16px;font-style:normal;font-variant-caps:normal;font-variant-ligatures:normal;font-weight:400;letter-spacing:normal;margin:16px 0px;orphans:2;text-align:start;text-decoration-color:initial;text-decoration-style:initial;text-indent:0px;text-transform:none;white-space:normal;widows:2;word-spacing:0px;'\u003e\u003cspan\u003eFile Name: \u003c\/span\u003e\u003ci\u003e\u003cspan\u003eBD Rhapsody™ System Library Preparation Protocol TCR\/BCR Full Length and mRNA Whole Transcriptome Analysis (WTA)\u003c\/span\u003e\u003c\/i\u003e\u003c\/p\u003e\n\u003cp class=\"ds-markdown-paragraph\" style='-webkit-text-stroke-width:0px;background-color:rgb(255, 255, 255);color:rgb(15, 17, 21);font-family:quote-cjk-patch, Inter, system-ui, -apple-system, BlinkMacSystemFont, \"Segoe UI\", Roboto, Oxygen, Ubuntu, Cantarell, \"Open Sans\", \"Helvetica Neue\", sans-serif;font-size:16px;font-style:normal;font-variant-caps:normal;font-variant-ligatures:normal;font-weight:400;letter-spacing:normal;margin:16px 0px;orphans:2;text-align:start;text-decoration-color:initial;text-decoration-style:initial;text-indent:0px;text-transform:none;white-space:normal;widows:2;word-spacing:0px;'\u003e\u003cspan\u003eDocument Number: 23-24017(03)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"ds-markdown-paragraph\" style='-webkit-text-stroke-width:0px;background-color:rgb(255, 255, 255);color:rgb(15, 17, 21);font-family:quote-cjk-patch, Inter, system-ui, -apple-system, BlinkMacSystemFont, \"Segoe UI\", Roboto, Oxygen, Ubuntu, Cantarell, \"Open Sans\", \"Helvetica Neue\", sans-serif;font-size:16px;font-style:normal;font-variant-caps:normal;font-variant-ligatures:normal;font-weight:400;letter-spacing:normal;margin:16px 0px;orphans:2;text-align:start;text-decoration-color:initial;text-decoration-style:initial;text-indent:0px;text-transform:none;white-space:normal;widows:2;word-spacing:0px;'\u003e\u003cspan\u003eDownload Link: \u003c\/span\u003e\u003ca style=\"border-bottom:2px solid rgba(var(--ds-rgba-transparent));border-left:3px solid rgba(var(--ds-rgba-transparent));border-right:3px solid rgba(var(--ds-rgba-transparent));border-top:2px solid rgba(var(--ds-rgba-transparent));color:var(--dsw-alias-brand-text);margin-left:-3px;margin-right:-3px;position:relative;text-decoration:none;transition:box-shadow var(--ds-transition-duration)var(--ds-ease-in-out);\" target=\"_blank\" rel=\"noreferrer\"\u003e\u003cspan\u003ehttps:\/\/scomix.bd.com\/hc\/en-us\/articles\/9285990045197-VDJ-full-length-TCR-BCR-assay-protocols-Human\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp class=\"ds-markdown-paragraph\" style='-webkit-text-stroke-width:0px;background-color:rgb(255, 255, 255);color:rgb(15, 17, 21);font-family:quote-cjk-patch, Inter, system-ui, -apple-system, BlinkMacSystemFont, \"Segoe UI\", Roboto, Oxygen, Ubuntu, Cantarell, \"Open Sans\", \"Helvetica Neue\", sans-serif;font-size:16px;font-style:normal;font-variant-caps:normal;font-variant-ligatures:normal;font-weight:400;letter-spacing:normal;margin:16px 0px 0px !important;orphans:2;text-align:start;text-decoration-color:initial;text-decoration-style:initial;text-indent:0px;text-transform:none;white-space:normal;widows:2;word-spacing:0px;'\u003e\u003cspan\u003eNote: In this kit, the amount of Bead RT\/PCR Enhancer is 40 µL, and the corresponding amount of Nuclease-Free Water is 45.2 µL.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"4T","offer_id":42302160306251,"sku":null,"price":1667.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_50ddf852-0048-4b7a-b6fb-656bcfe31cf0.png?v=1776073538","url":"https:\/\/www.antbioinc.com\/products\/bcr-flbd-ua086054","provider":"AntBio","version":"1.0","type":"link"}