{"product_id":"bax-knockout-hela-cell-lysate-s0y0028","title":"Bax-Knockout HeLa Cell Lysate","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMutations\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eMutation in exon3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eWB\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e2 mg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eSupplied in SDS Sample Buffer: 50 mM Tris-HCl (pH 6.8 at 25°C), 2% w\/v SDS, 10% glycerol, 1% v\/v β-Mercaptoethanol, 0.01% w\/v bromophenol blue or phenol red.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-20 °C for 1 months under sterile conditions; \u003cbr\u003e-80 °C for 24 months under sterile conditions.\u003cbr\u003ePlease avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan\u003eBax (Bcl-2-associated X protein) is a pro-apoptotic member of the Bcl-2 protein family and a key regulator of the intrinsic, mitochondria-mediated apoptotic pathway. In response to cellular stress, Bax undergoes conformational activation and translocates from the cytosol to the mitochondrial outer membrane, where it oligomerizes with other pro-apoptotic Bcl-2 family proteins to promote mitochondrial outer membrane permeabilization and the release of cytochrome c. Cytochrome c subsequently contributes to apoptosome formation and activation of caspase-9 and downstream executioner caspases, including caspase-3, ultimately leading to apoptotic cell death. Bax antibodies are widely used in Western blotting, immunohistochemistry, immunofluorescence, and flow cytometry to assess Bax expression, subcellular localization, and apoptosis-related signaling. Caspase-3 knockout may inhibit or delay the downstream execution phase of apoptosis and increase cellular resistance to apoptosis-inducing stimuli, while Bax activation and mitochondrial apoptotic signaling may remain relatively intact, depending on the cell type and apoptotic stimulus.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1kit","offer_id":43160888344651,"sku":"S0Y0028-1kit","price":665.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/8c119f69cb494a82ba08b75b32f3d02d.jpg?v=1788768060","url":"https:\/\/www.antbioinc.com\/products\/bax-knockout-hela-cell-lysate-s0y0028","provider":"AntBio","version":"1.0","type":"link"}