{"product_id":"advanced-dmem-f-12-dulbeccos-modified-eagle-medium-hams-f-16-medium-abs90041","title":"Advanced DMEM\/F-12 (Dulbecco's Modified Eagle Medium\/Ham's F-16) Medium","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eInstructions for use\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eUsing the colon cancer organoid medium configuration as a reference: add to 500ml of organoid basal medium : N2 (abs9121-5mL), B27 (abs9272-10mL), R-spondin1 (abs04398-50ug), Noggin (abs04695-50ug), EGF (abs05152-100ug), FGF10, Glutamax (abs9295-100mL), P\/S (abs9244-100mL), HEPES (abs42150994-100mL), N-Acetylcysteine (abs822057-10mg), Nicotinamide (abs816969-200mg), A8301 (abs817694-25mg), Gastrin (abs814916-1mg), SB202190 (abs810009-50mg), Y27632 (abs812864-50mg), Can be directly used for intestinal cancer organoid culture.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProduct Advantage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eSpecially developed basal medium for organoid culture, produced in accordance with GMP regulations, with stable process and small batch benefit.\u003c\/li\u003e\n\u003cli\u003eEffectively increases the cell proliferation rate under serum-reduced culture without affecting cell viability and morphology.\u003c\/li\u003e\n\u003cli\u003eAll raw materials are selected from self-produced or strictly tested and purified low-endotoxin high-purity medicinal grade raw materials, which have low pyrogen levels and reduce the interference of exogenous endotoxins.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eOrganoid basal culture medium is a serum-reduced basal culture medium that requires the addition of related protein factors and inhibitors such as R-spondin1, EGF, B27, N2, and Y27632. It can be directly used for various organoid cultures, and can also be used to cultivate mammalian cells with a reduced amount of fetal bovine serum (FBS).\u003c\/p\u003e\n\u003cp\u003eThe organoid basal medium was supplemented with: insulin, transferrin, bovine serum albumin, ethanolamine, ascorbic acid, glutathione, bispecific antibodies, and various trace elements (sodium selenite, ammonium metavanadate, manganese chloride, and copper sulfate).\u003c\/p\u003e\n\u003cp\u003eThis product contains: D-glucose, non-essential amino acids, phenol red, sodium pyruvate.\u003c\/p\u003e\n\u003cp\u003eDoes not contain: L-glutamine, HEPES.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eStore at 4℃ for 1 year\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"500mL","offer_id":43224150048843,"sku":"abs90041-500mL","price":63.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.antbioinc.com\/products\/advanced-dmem-f-12-dulbeccos-modified-eagle-medium-hams-f-16-medium-abs90041","provider":"AntBio","version":"1.0","type":"link"}