{"product_id":"acss1-his-tag-protein-human-ua085075","title":"ACSS1 His Tag Protein, Human","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eACSS1, ACAS2L, KIAA1846, Acetate--CoA ligase 2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAccession\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eQ9NUB1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAmino Acid Sequence\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eAla38-Lys689 with His Tag at the N-Terminus\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eE.coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e70-100kDa (Reducing)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e＞95% by SDS-PAGE \u0026amp; HPLC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHis Tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e50mM Tris, 150mM NaCl, pH7.5, 1mM DTT, 10%Glycerol\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eStable for 12 months upon stored at -80\u003c\/span\u003e\u003cspan\u003e℃\u003c\/span\u003e\u003cspan\u003e from the date of receipt. And avoid repeated freeze-thaws cycles.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e1. Fu, X., et al. (2025). ACSS1 co-opts acetyl-CoA metabolism to drive DNA repair and undermine radiotherapy efficacy in breast cancer. Cell Death \u0026amp; Disease, 17, 119.\u003cbr\u003e2. Schug, Z.T., et al. (2015). Acetyl-CoA synthetase 2 promotes acetate utilization and maintains cancer cell growth under metabolic stress. Cancer Cell, 27(1), 57-71.\u003cbr\u003e3. Fujino, T., et al. (2001). Acetyl-CoA synthetase 2, a mitochondrial matrix enzyme involved in the oxidation of acetate. Journal of Biological Chemistry, 276(14), 11420-11426.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003eACSS1 (Acyl-CoA Synthetase Short-Chain Family Member 1) is a key metabolic enzyme localized in the mitochondrial matrix. Encoded by the human ACSS1 gene on chromosome 20p11.21, the protein is 689 amino acids long and features an N-terminal acetyl-CoA synthetase domain and two AMP-binding domains.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eFunctionally, ACSS1 catalyzes the ATP-dependent conversion of acetate and CoA into acetyl-CoA, a critical step in mitochondrial energy metabolism that supplies the TCA cycle. Under glucose deprivation, ACSS1 utilizes alternative carbon sources like acetate to sustain energy production in high-energy organs such as the heart and brain. Its enzymatic activity is regulated by SIRT3-mediated deacetylation at a key lysine residue (K642 in humans).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eDysregulation of ACSS1 expression and function is implicated in various diseases. In oncology, ACSS1 is aberrantly overexpressed in breast cancer, AML, and melanoma, where it rewires acetyl-CoA metabolism to drive DNA repair, promote tumor growth and metastasis, and is associated with poor prognosis and therapy resistance. Furthermore, reduced ACSS1 function may contribute to energy failure in neurodegenerative diseases and is linked to metabolic disorders including nonalcoholic fatty liver disease and sarcopenia.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"10μg","offer_id":42887926972491,"sku":"UA085075-10μg","price":200.0,"currency_code":"USD","in_stock":true},{"title":"50μg","offer_id":42887927005259,"sku":"UA085075-50μg","price":560.0,"currency_code":"USD","in_stock":true},{"title":"100μg","offer_id":42887927038027,"sku":"UA085075-100μg","price":955.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_23606078-0a46-486f-9e4d-a720bf78757c.png?v=1783774842","url":"https:\/\/www.antbioinc.com\/products\/acss1-his-tag-protein-human-ua085075","provider":"AntBio","version":"1.0","type":"link"}