{"product_id":"acheacetylcholinesterase-his-tag-protein-human-ua011275","title":"ACHE\/Acetylcholinesterase His Tag Protein, Human","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eARACHE,apoptosis-related acetylcholinesterase,N-ACHE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAccession\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eP22303-1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAmino Acid Sequence\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eGlu32-Leu614 with His Tag at the C-Terminus\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHEK293\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e65-80kDa (Reducing)\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e＞90% by SDS-PAGE \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHis Tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLyophilized powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003ePBS, PH7.4, 5% trehalose\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReconstitution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003eReconstitute at 0.1-1 mg\/ml according to the size in ultrapure water after rapid centrifugation.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.\u003cbr\u003e· 3 months, -20 to -80℃ under sterile conditions after reconstitution.\u003cbr\u003e· 1 week, 2 to 8℃ under sterile conditions after reconstitution.\u003cbr\u003e· Please avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e1.Rawat K, Tewari D, Bisht A, Chandra S, Tiruneh YK, Hassan HM, Al-Emam A, Sindi ER, Al-Dies AM. Identification of AChE targeted therapeutic compounds for Alzheimer's disease: an in-silico study with DFT integration. Sci Rep. 2024 Dec 5;14(1):30356.\u003cbr\u003e2.Reynoso-García MF, Nicolás-Álvarez DE, Tenorio-Barajas AY, Reyes-Chaparro A. Structural Bioinformatics Applied to Acetylcholinesterase Enzyme Inhibition. Int J Mol Sci. 2025 Apr 17;26(8):3781.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eAcetylcholinesterase (AChE), also known as acetylhydrolase, is a crucial serine hydrolase that efficiently terminates signal transmission at neuromuscular junctions and cholinergic synapses by rapidly hydrolyzing acetylcholine (ACh) in the synaptic cleft. Beyond its fundamental role in neurotransmitter regulation, AChE has been found to participate in neuronal apoptosis and other cellular processes, suggesting a broader involvement in programmed cell death. In neurodegenerative diseases such as Alzheimer's disease, acetylcholine deficiency is closely associated with cognitive decline, making AChE an important therapeutic target. Currently, acetylcholinesterase inhibitors (AChEIs) are widely used in clinical practice for the targeted treatment of Alzheimer's disease and other forms of dementia.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"text-align:center;\"\u003e \u003c\/p\u003e\n\u003cp style=\"line-height:150%;text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;line-height:150%;'\u003e\u003cstrong\u003eExperimental Method\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"line-height:150%;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;line-height:150%;'\u003e\u003cstrong\u003ePrinciple\u003c\/strong\u003e: Detect the ability of ACHE protein to cleave Acetylthiocholine.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"line-height:150%;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;line-height:150%;'\u003e\u003cstrong\u003eMaterials\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003col style=\"padding-left:24px;\"\u003e\n\u003cli\u003e\u003cp style=\"line-height:150%;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;line-height:150%;'\u003eAssay buffer: \u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;line-height:150%;' lang=\"EN-US\"\u003e0.1 M Sodium Phosphate, 0.05% (w\/v) Brij-35, pH 7.5\u003c\/span\u003e\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eACHE\/Acetylcholinesterase His Tag Protein, Human (SPA1701)\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp style=\"line-height:150%;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;line-height:150%;'\u003eSubstrate: \u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;line-height:150%;' lang=\"EN-US\"\u003eAcetylthiocholine (ATC) (A5626)\u003c\/span\u003e\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp style=\"line-height:150%;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;line-height:150%;' lang=\"EN-US\"\u003e5’,5’-Dithiobis (2-nitrobenzoic acid) (DTNB) (D8130)\u003c\/span\u003e\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp style=\"line-height:150%;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;line-height:150%;' lang=\"EN-US\"\u003e96 Well Clear Plate (701311)\u003c\/span\u003e\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp style=\"line-height:150%;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;line-height:150%;'\u003ePlate reader (\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;line-height:150%;' lang=\"EN-US\"\u003ePerkinElmer\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;line-height:150%;'\u003e, \u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;line-height:150%;' lang=\"EN-US\"\u003e405 nm\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;line-height:150%;'\u003e)\u003c\/span\u003e\u003c\/p\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp style=\"line-height:150%;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;line-height:150%;'\u003e\u003cstrong\u003eProcedure\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003col style=\"padding-left:24px;\"\u003e\n\u003cli\u003e\u003cp style=\"line-height:150%;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;line-height:150%;'\u003eDilute ACHE protein to \u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;line-height:150%;' lang=\"EN-US\"\u003e0.02 μg\/mL\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;line-height:150%;'\u003e in assay buffer.\u003c\/span\u003e\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp style=\"line-height:150%;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;line-height:150%;'\u003eDilute substrate to \u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;line-height:150%;' lang=\"EN-US\"\u003e200 μM\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;line-height:150%;'\u003e in assay buffer containing \u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;line-height:150%;' lang=\"EN-US\"\u003e100 μM DTNB\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;line-height:150%;'\u003e.\u003c\/span\u003e\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp style=\"line-height:150%;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;line-height:150%;'\u003eAdd \u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;line-height:150%;' lang=\"EN-US\"\u003e50 μL\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;line-height:150%;'\u003e of diluted ACHE protein to the clear plate. Include a control group with assay buffer instead of ACHE protein.\u003c\/span\u003e\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp style=\"line-height:150%;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;line-height:150%;'\u003eAdd \u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;line-height:150%;' lang=\"EN-US\"\u003e50 μL\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;line-height:150%;'\u003e of substrate\/DTNB mixture to initiate the reaction.\u003c\/span\u003e\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp style=\"line-height:150%;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;line-height:150%;'\u003eRead the signal at \u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;line-height:150%;' lang=\"EN-US\"\u003e405 nm\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;line-height:150%;'\u003e in kinetic mode for \u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;line-height:150%;' lang=\"EN-US\"\u003e10\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;line-height:150%;'\u003e minutes.\u003c\/span\u003e\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp style=\"line-height:150%;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;line-height:150%;'\u003eCalculate specific activity:\u003c\/span\u003e\u003c\/p\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;\" border=\"0\" cellspacing=\"0\" cellpadding=\"0\" width=\"574\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding:7.5pt 7.5pt 7.5pt 7.5pt;width:82.7pt;\" rowspan=\"2\" width=\"110\"\u003e\u003cp style=\"line-height:150%;margin-bottom:7.5pt;text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;line-height:150%;' lang=\"EN-US\"\u003eSpecific Activity (pmol\/min\/µg) =\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border:none;padding:7.5pt 7.5pt 1.5pt 7.5pt;width:347.6pt;\" width=\"463\"\u003e\u003cp style=\"line-height:150%;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;line-height:150%;' lang=\"EN-US\"\u003eAdjusted Vmax* (OD\/min) x well volume (L) x 10\u003csup\u003e9\u003c\/sup\u003e nmol\/mol\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border:none;padding:7.5pt 7.5pt 7.5pt 7.5pt;width:347.6pt;\" width=\"463\"\u003e\n\u003cp style=\"line-height:150%;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;line-height:150%;' lang=\"EN-US\"\u003eext. coeff** (M\u003csup\u003e--1\u003c\/sup\u003ecm\u003csup\u003e--1\u003c\/sup\u003e) x path corr.*** (cm) \u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"line-height:150%;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;line-height:150%;' lang=\"EN-US\"\u003ex amount of enzyme (μg)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp style=\"line-height:150%;\"\u003e\u003cspan style=\"color:white;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;line-height:150%;' lang=\"EN-US\"\u003e*\u003c\/span\u003e\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;line-height:150%;'\u003eSubstrate blank correction\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"line-height:150%;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;line-height:150%;' lang=\"EN-US\"\u003e**\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;line-height:150%;'\u003eExtinction coefficient \u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;line-height:150%;' lang=\"EN-US\"\u003e13260 M\u003c\/span\u003e\u003cspan style='font-family:\"MS Gothic\";font-size:11.0pt;line-height:150%;'\u003e⁻\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;line-height:150%;' lang=\"EN-US\"\u003e¹cm\u003c\/span\u003e\u003cspan style='font-family:\"MS Gothic\";font-size:11.0pt;line-height:150%;'\u003e⁻\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;line-height:150%;' lang=\"EN-US\"\u003e¹\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e***\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;'\u003ePath length correction \u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e0.32 cm\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"25μg","offer_id":42332763750475,"sku":null,"price":415.0,"currency_code":"USD","in_stock":true},{"title":"100μg","offer_id":42332763783243,"sku":null,"price":830.0,"currency_code":"USD","in_stock":true},{"title":"500μg","offer_id":42332763816011,"sku":null,"price":1985.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_a82b4d79-8cbb-4444-92b2-a1d154c6df4d.png?v=1780633724","url":"https:\/\/www.antbioinc.com\/products\/acheacetylcholinesterase-his-tag-protein-human-ua011275","provider":"AntBio","version":"1.0","type":"link"}