{"product_id":"3d-cell-spheroid-hydrogel-kit-abs90548","title":"3D Cell Spheroid Hydrogel Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e 1. Reagent preparation steps: \u003c\/strong\u003e\u003cbr\u003e1 、 Gel  At  37℃ water bath preheating  10 min Confirm complete melting, and place before use.  37℃ The water bath can reduce the viscosity of the colloid. \u003cbr\u003e2 、 Buffer (1X) ：   Use  4℃ DMEM  The culture medium will  Buffer (10X)  Dilute into  Buffer (1X) Place in the refrigerator or on crushed ice before use. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e II. 3D  cell sphere - Droplet Culture Steps: \u003cbr\u003e\u003c\/strong\u003e All steps were performed in a sterile operating bench, and the operating steps were as follows: \u003cbr\u003e1 Prepared using complete culture medium containing  2×10\u003csup\u003e5\u003c\/sup\u003e~10\u003csup\u003e7\u003c\/sup\u003ecells\/mL  cell suspension and  Gel  According to  1 ： 1  Uniformly mix to prepare a cell suspension, so that the final cell density is  10\u003csup\u003e5\u003c\/sup\u003e~10\u003csup\u003e7\u003c\/sup\u003ecells\/mL , get  Gel  Cell suspension. \u003cbr\u003e\u003cstrong\u003e Note  1 ： A Gel  The solution is below  22℃ It is easy to form a gel and melt; please proceed promptly. \u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e Note  2 If different hardness requirements are required, please refer to the colloid hardness adjustment ratio.  Q\u0026amp;A 。 \u003c\/strong\u003e\u003cbr\u003e2 , take  20 ~ 40 μL  Gel  The cell suspension is dropped into the cell culture plate, and the culture plate is carefully transferred to crushed ice or  4℃ Inside the refrigerator, glue droplets  5 min  Internal gel formation. \u003cbr\u003e\u003cstrong\u003e Note  3 It is recommended that the gel droplets be as small and round as possible. \u003c\/strong\u003e\u003cbr\u003e3 After the gel droplets form gel, they are added to the culture plate along the sidewall of the well.  1 mL  pre-cooled  Buffer (1X)  Cover with glue droplets and let stand on crushed ice.  15 min Crosslinking. \u003cbr\u003e4 After crosslinking,  Buffer (1X)  Aspirate out and replace with complete medium suitable for cell growth. \u003cbr\u003e5 Place the cell culture plate in  37 °C  Culture in carbon dioxide incubator 5~14  Days, and observe the formation of cell spheres. Perform the medium change operation according to the normal medium change frequency. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e III. Sols and Collection of Cell Spheres: \u003c\/strong\u003e\u003cbr\u003e If required, the cell recovery solution can be ordered separately (item number: abs90549 ） \u003cbr\u003e1 Reagent preparation: \u003cbr\u003e cell recovery solution  (1X) : Use  4℃ PBS (1X) Cell recovery solution (10X) Diluted celluloblast recovery solution  (1X) 。 \u003cbr\u003e2 Collecting Cell Spheres: \u003cbr\u003e （ 1 Carefully aspirate and remove the culture medium, and use...  PBS (1X) Perform cleaning. will  PBS  Remove by suction and add  1 mL  cell recovery solution  (1X) Cover the gel droplets and react at room temperature.  5 min 。 \u003cbr\u003e （ 2 Gently blow the gel droplets with a pipette until the gel droplets are completely dissolved. \u003cbr\u003e （ 3 The solution containing cell spheres is drawn into a centrifuge tube to...  250g  centrifuge  5 min The supernatant was removed and the pelleted cell spheres were collected for subsequent experimental analysis. \u003cbr\u003e3 Collection of single cells: \u003cbr\u003e （ 1 Before separating the cell mass, follow the steps described above. “ Sols and Collection of Cell Spheres ” The method is used to perform the operation. \u003cbr\u003e （ 2 ) Add  0.25% Pancreatin ( abs47014938  ) and the collected cell spheres in  37°C  Mixing reaction. use  1 mL  The pipette gently blows the cell pellet until the cell spheres are completely digested. \u003cbr\u003e3 After the cell spheres are completely digested, add...  3  double volume  PBS (1X) , with  250g  centrifuge  5 min The supernatant was removed and the pelleted single cells were collected for subsequent experimental analysis. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e3D  The cell sphere hydrogel kit contains a complete set of colloids and reagents, the polymer colloids of which can quickly form hydrogels.  3D Applications related to cell culture and microenvironment research. This kit is convenient to operate and can regulate colloidal hardness according to the characteristics of the source tissue for various cell culture tests. \u003cbr\u003e\u003cstrong\u003e Product Composition: \u003c\/strong\u003e\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 95%; height: 66px; margin-left: auto; margin-right: auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 19.3431%; text-align: center; height: 22px;\"\u003e\u003cstrong\u003e Product Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 26.7823%; text-align: center; height: 22px;\"\u003e\u003cstrong\u003e5mL\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.0627%; text-align: center; height: 22px;\"\u003e\u003cstrong\u003e10mL\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.0627%; text-align: center; height: 22px;\"\u003e\u003cstrong\u003e Preserve \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 19.3431%; text-align: center; height: 22px;\"\u003eGel\u003c\/td\u003e\n\u003ctd style=\"width: 26.7823%; text-align: center; height: 22px;\"\u003e5mL\u003c\/td\u003e\n\u003ctd style=\"width: 23.0627%; text-align: center; height: 22px;\"\u003e10mL\u003c\/td\u003e\n\u003ctd style=\"width: 23.0627%; text-align: center; height: 22px;\"\u003e-20℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 19.3431%; text-align: center; height: 22px;\"\u003eBuffer(10X)\u003c\/td\u003e\n\u003ctd style=\"width: 26.7823%; text-align: center; height: 22px;\"\u003e25mL\u003c\/td\u003e\n\u003ctd style=\"width: 23.0627%; text-align: center; height: 22px;\"\u003e50mL\u003c\/td\u003e\n\u003ctd style=\"width: 23.0627%; text-align: center; height: 22px;\"\u003e-20℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cstrong\u003e Note: \u003c\/strong\u003e\u003cbr\u003e1 This product is shipped with blue ice. Please transfer it to the appropriate temperature as soon as possible after receiving it according to the product's storage requirements. This product is recommended to be used immediately after thawing. If not for immediate use, please repackage and store. \u003cbr\u003e2 Please use this product within six months after opening. \u003cbr\u003e\u003cstrong\u003e Applicable cell types: \u003c\/strong\u003e\u003cbr\u003e1 Normal cell lines \u003cbr\u003e2 tumor cell lines, \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at -20℃ for 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e3D cell sphere culture test; 3D cell sphere drug screening platform; Cancer cell line invasion assay; Hydrogel Coating Test\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"5mL","offer_id":43224140972107,"sku":"abs90548-5mL","price":244.0,"currency_code":"USD","in_stock":true},{"title":"10mL","offer_id":43224141004875,"sku":"abs90548-10mL","price":459.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.antbioinc.com\/products\/3d-cell-spheroid-hydrogel-kit-abs90548","provider":"AntBio","version":"1.0","type":"link"}