{"product_id":"10xc-ua085056","title":"10x Kinase Buffer C","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eFor long term storage, keep 10X buffer at -20°C. If buffer will be continually used, it is recommended that the 10X buffer be kept at 4°C for 1-2 weeks. For optimal storage, aliquot buffer into smaller quantities after centrifugation and store at recommended temperature. For most favorable \u003cbr\u003eperformance, avoid repeated handling and multiple freeze\/thaw cycles.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan\u003eKise Buffer can be used to assay protein kise activity.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan\u003e250 mM Tris-HCl (pH 7.5), 50 mM beta-glycerophosphate, 20 mM dithiothreitol (DTT), 1 mM \u003c\/span\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003eVO\u003c\/span\u003e\u003cspan\u003e4\u003c\/span\u003e\u003cspan\u003e, 100 mM MgCl\u003c\/span\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003e1. Thaw 10X buffer at 24-30°C, mixing end-over-end.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2. Dilute the 10X buffer to the desired concentration using ddH\u003c\/span\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003eO.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eGuidelines\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"margin-bottom: 0px;\"\u003eKise Buffer must be supplemented with ATP.\u003c\/p\u003e\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"1mL","offer_id":42302218960971,"sku":null,"price":25.0,"currency_code":"USD","in_stock":true},{"title":"5mL","offer_id":42302218993739,"sku":null,"price":85.0,"currency_code":"USD","in_stock":true},{"title":"10mL","offer_id":42302219026507,"sku":null,"price":135.0,"currency_code":"USD","in_stock":true},{"title":"20mL","offer_id":42302219059275,"sku":null,"price":175.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_a209e673-c219-4988-b9be-6c32ce1f6d2b.png?v=1776073873","url":"https:\/\/www.antbioinc.com\/products\/10xc-ua085056","provider":"AntBio","version":"1.0","type":"link"}