{"title":"TR-FRET Kit","description":null,"products":[{"product_id":"human-stat6crbn-protac-binding-kit-ua085003","title":"UniOne® TR-FRET Human STAT6\/CRBN PROTAC Binding Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-80℃\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003eThe kit employs homogeneous time-resolved fluorescence (TR-FRET) technology to measure the interaction between molecular glue AK-1690 and test compounds mediating STAT6 and CRBN. This method enables high-throughput screening of small molecules capable of mediating the interaction between STAT6 and CRBN in a simple and rapid manner.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAs shown, the interaction between STAT6 and CRBN is detected using Eu-labeled anti-Tag1 antibody (TR-FRET donor) and Ac-labeled anti-Tag2 antibody (TR-FRET acceptor). Since molecular glue AK-1690 mediates the interaction between STAT6 and CRBN, the proximity of the donor and acceptor antibodies allows fluorescence resonance energy transfer (FRET) from the donor to the acceptor upon excitation, resulting in a specific emission signal at 665 nm. This signal is proportional to the extent of interaction mediated by AK-1690 between STAT6 and CRBN. The homogeneous assay is simple to perform and requires no washing steps.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/ea03772f-6e06-4254-8d49-8ec8aa6d7d4b.png\" style=\"margin-left:30px; width:393px; height:auto; vertical-align:middle;\" alt=\"\"\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003ctable border=\"1\" style=\"border-collapse:collapse;width:auto;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eComponent\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eConcentration\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"\"\u003e100T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"\"\u003e500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"\"\u003e2500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"\"\u003e10000T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eStorage Temperature\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag1-STAT6 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e40μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e200μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e800μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag2- CRBN protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:极1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e40μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e200μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e800μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAK-1690 (150μM)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #极000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e200μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e极p\u0026gt;\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAnti-Tag1 Eu antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e250μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1000μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAnti-Tag2 Ac antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e12.5×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e40μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e200μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:极center\"\u003e\u003cspan\u003e1000μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4000μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #极000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000极;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e40mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003e.\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003e Reagent Preparation\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003e1\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e.1 \u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eThaw all reagents at room temperature before use (equilibrate at room temperature for at least 30 min). The reaction volume for the 384-well shallow plate is 20\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eμ\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eL (reagent volumes for the reaction system are shown in the table). Calculate the required volume for the experiment before preparation and prepare as needed; the following preparation is for reference only, using 500 assays as an example.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"color:#000000\"\u003eTable 1\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e. \u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eReagent Preparation\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eReagent Name\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003ePreparation\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eVolume per Assay Well (μL)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDetection buffer 1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan\u003eTake\u003c\/span\u003e\u003cspan\u003e2mL 10\u003c\/span\u003e\u003cspan\u003e ×\u003c\/span\u003e\u003cspan\u003e Detection buffer1\u003c\/span\u003e\u003cspan\u003eand add\u003c\/span\u003e\u003cspan\u003e18mL deionized water to dilute\u003c\/span\u003e\u003cspan\u003eto\u003c\/span\u003e\u003cspan\u003e1×. Mix\u003c\/span\u003e\u003cspan\u003ewell and set\u003c\/span\u003e\u003cspan\u003easide\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAK-1690\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp\u003e\u003cspan\u003eAccording to the reaction system, dilute the compound with\u003c\/span\u003e\u003cspan\u003e1×Detection buffer to the desired testing concentration\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eEnsure consistent\u003c\/span\u003e\u003cspan\u003eDMSO concentration across all assay wells\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag1-STAT6 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan\u003eTake\u003c\/span\u003e\u003cspan\u003e40μL Tag1-STAT6 protein stock solution and dilute to 2mL with 1× Detection buffer.\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eMix well and set aside.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag2- CRBN protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan\u003eTake\u003c\/span\u003e\u003cspan\u003e40μL Tag2-CRBN protein stock solution and dilute to 2mL with 1×Detection buffer.\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eMix well and set aside.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAntibody Mix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan\u003eTake\u003c\/span\u003e\u003cspan\u003e50μL Anti-Tag1 Eu antibody \u003c\/span\u003e\u003cspan\u003estock solution and add\u003c\/span\u003e\u003cspan\u003e 1×Detection buffer 2.45mL.\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eMix well;\u003c\/span\u003e\u003cspan\u003etake\u003c\/span\u003e\u003cspan\u003e200μL Anti-Tag2 Ac antibody \u003c\/span\u003e\u003cspan\u003estock solution and add\u003c\/span\u003e\u003cspan\u003e 1× Detection buffer 2.3mL.\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eMix well;\u003c\/span\u003e\u003cspan\u003emix the two solutions at a\u003c\/span\u003e\u003cspan\u003e1:1 ratio to prepare the Antibody Mix.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan style=\"color:#000000\"\u003e1.2 \u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eGradient Dilution of Test Samples\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eUsing AK-1690 as an example, the diluent is 1× Detection buffer. To minimize the impact of matrix effects, it is recommended to dilute with a solution matching the matrix of the test sample; adjust the test sample concentrations according to actual conditions.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"color:#000000\"\u003eTable\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e2. AK-1690\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e Gradient Dilution\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e (Adjust according to actual conditions)\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eFinal Concentration of AK-1690 (nM)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003ePreparation Concentration of AK-1690 (nM)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003ePreparation Method\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e①\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e150\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e1500\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003eμL 150μM \u003c\/span\u003e\u003cspan\u003eAK-1690 \u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e99\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e②\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e100\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e1000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003e0μL \u003c\/span\u003e\u003cspan\u003e①\u003c\/span\u003e\u003cspan\u003e +\u003c\/span\u003e\u003cspan\u003e15\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e③\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e66.67 \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e666.67 \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003e0μL \u003c\/span\u003e\u003cspan\u003e②\u003c\/span\u003e\u003cspan\u003e +\u003c\/span\u003e\u003cspan\u003e15\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e④\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e44.44 \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e444.44 \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003e0μL \u003c\/span\u003e\u003cspan\u003e③\u003c\/span\u003e\u003cspan\u003e +\u003c\/span\u003e\u003cspan\u003e15\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑤\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e29.63 \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e296.30 \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003e0μL \u003c\/span\u003e\u003cspan\u003e④\u003c\/span\u003e\u003cspan\u003e +\u003c\/span\u003e\u003cspan\u003e15\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑥\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e19.75 \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e197.53 \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003e0μL \u003c\/span\u003e\u003cspan\u003e⑤\u003c\/span\u003e\u003cspan\u003e +\u003c\/span\u003e\u003cspan\u003e15\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑦\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e13.17 \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e131.69 \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003e0μL \u003c\/span\u003e\u003cspan\u003e⑥\u003c\/span\u003e\u003cspan\u003e +\u003c\/span\u003e\u003cspan\u003e15\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑧\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e8.78 \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e87.79 \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003e0μL \u003c\/span\u003e\u003cspan\u003e⑦\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e15\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑨\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e5.85 \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e58.53 \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003e0μL \u003c\/span\u003e\u003cspan\u003e⑧\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e15\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eBlank\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003e0μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003e2\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003e.\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003e Sample Addition and Controls\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003e2.1 Test Samples: Add 2μL of gradient-diluted test sample, 4μL of Tag1-STAT6 protein working solution, 4μL of Tag2-CRBN protein working solution, and 10μL of mixed Antibody Mix (Tag2-CRBN protein can be mixed with Antibody Mix at a proportional ratio before addition) sequentially into the 384-well shallow plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003e2.2  Positive Control Standard Curve: 2μL of gradient-diluted AK-1690, 4μL of Tag1-STAT6 protein working solution, 4μL of Tag2-CRBN protein working solution, and 10μL of Antibody Mix.\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse:collapse;width:auto;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003ePositive Control Standard Curve\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eTest Samples\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eBlank Control Wells\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNC\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"2\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eStep 1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eGradient-diluted\u003c\/span\u003e\u003cspan\u003eAK-1690\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eGradient-diluted Test Samples\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"4\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e0\u003c\/span\u003e\u003cspan\u003eμL 1\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003cspan\u003e Detection buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 10μL Antibody Mix\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4μL Tag1- STAT6 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eStep 2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4μL Tag2-CRBN protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10μL Antibody Mix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"4\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eSeal the plate wells with a sealing film\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e.\u003c\/span\u003e\u003cspan\u003e Incubate at room temperature for 2 hours;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan style=\"color:#000000\"\u003e2.3 Blank Control Wells: Replace test samples with 2μL of 1× Detection buffer;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003e2.4 NC: 10μL of 1× Detection buffer plus 10μL of Antibody Mi\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003ex\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003eAfter adding all samples, centrifuge, seal with a sealing film, and incubate at room temperature for 2 hours.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e3.\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003e Detection\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003eDetect using a microplate reader compatible with TR-FRET\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e.\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e Excitation wavelength is 320\/340nm, and emission wavelengths are 620nm and 665nm.\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e[Result Calculation]\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003e1)\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e Calculate the signal value\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e (Ratio)\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e: Divide the 665nm fluorescence signal by the 620nm fluorescence signal, then multiply by 10000\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"color:#000000\"\u003eRatio\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e \u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e=\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e \u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e(665\/620)\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e ×\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e10000\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003e2)\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e Calculate\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eNet signal\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e based on the signal value: \u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"color:#000000\"\u003eNet signal\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e = (Std-\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eNC\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e)\/\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eNC\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e×100\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003e3)\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e Calculate CV（%）\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"color:#000000\"\u003eCV（%）= Standard Deviation\/Mean Ratio × 100%\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e[Data Example]\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003eThe following data cannot replace data obtained from actual experiments and is provided for illustration only. Results may vary depending on the plate reader.\u003c\/span\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/91c17fac-7963-4ecc-b512-2ee55dff5bc2.jpeg\" style=\"margin-left:30px; width:464px; height:auto; vertical-align:middle;\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003eNote: Recommended microplate (384-well plate, white, shallow well)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"100T","offer_id":43088829481035,"sku":"UA085003-100T","price":730.0,"currency_code":"USD","in_stock":true},{"title":"500T","offer_id":41887881855051,"sku":"UA085003-500T","price":2180.0,"currency_code":"USD","in_stock":true},{"title":"2500T","offer_id":41887881887819,"sku":"UA085003-2500T","price":8175.0,"currency_code":"USD","in_stock":true},{"title":"10000T","offer_id":41887881920587,"sku":"UA085003-10000T","price":21800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_cfe23af2-3739-4b19-b328-b146b32b1187.png?v=1788519701"},{"product_id":"unionetr-frethumaniggsandwichassaykit-ua085006","title":"UniOne® TR-FRET Human IgG Sandwich Assay Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-80℃\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003eThe kit employs homogeneous time-resolved fluorescence (HTRF) technology for the precise measurement of human IgG antibody or hFc-tagged protein concentrations in cell culture supernatants. The kit includes an antibody-labeled donor Eu (Anti-human IgG-Eu, Donor) and another antibody-labeled acceptor Ac (Anti-Human IgG-Ac, Acceptor) targeting a different epitope. When Anti-human IgG-Eu and Anti-human IgG-Ac bind to Human IgG, the Donor releases energy under 320 nm excitation and transfers it to the Acceptor, which then emits light at a specific wavelength (665 nm).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003eIn the kit, Anti-human IgG-Eu and Anti-human IgG-Ac form a sandwich complex with Human IgG in samples or standards, leading to an increase in the 665 nm signal. This signal is proportional to the concentration of Human IgG in the standards, enabling accurate quantification of Human IgG in samples via a standard curve. The homogeneous assay is simple to perform, requiring no washing steps, and can be completed in a single step.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003cspan\u003e    \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/6fa0fb00-17fc-4f67-95af-68b7f6e1b354.png\" style=\"margin-left:30px; width:598px; height:auto; vertical-align:middle;\" alt=\"\"\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003eTable 1. Kit Components\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse:collapse;width:auto;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eComponent\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eConcentration\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e100 Tests\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e500 Tests\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e2,500 Tests\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e10,000 Tests\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eStorage Temperature\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAnti\u003c\/span\u003e\u003cspan\u003e-\u003c\/span\u003e\u003cspan\u003eHuman IgG-Eu\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e250\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAnti\u003c\/span\u003e\u003cspan\u003e-\u003c\/span\u003e\u003cspan\u003eHuman IgG-Ac\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e250\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIgG Standard\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e40μg\/m\u003c\/span\u003e\u003cspan\u003el\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e200\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e40\u003c\/span\u003e\u003cspan\u003e0μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDetection Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1.5mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e6ml\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e3\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e0\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003emL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e1\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e20\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003emL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDiluent Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1.5mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e6ml\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e3\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e0\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003emL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e1\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e20\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003emL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan\u003eCaution: After the first thaw, immediately aliquot and store at the recommended temperature. Avoid storing diluted samples or subjecting them to repeated freeze-thaw cycles.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"margin-right:8px;\"\u003e1.\u003c\/span\u003e\u003cstrong\u003eReagent Preparation\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003eThaw all reagents at room temperature before use (equilibrate at room temperature for at least 30 min). The reaction volume for the 384-well shallow plate is 20 μL (reagent volumes for the reaction system are shown in the table). Calculate the required volume for the experiment before preparation and prepare as needed; the following preparation is for reference only, using 500 assays as an example.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"margin-right:8px;\"\u003e1.1\u003c\/span\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003e Reagent Preparation:\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan\u003eTable 2. Reagent Preparation and Volumes\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:1px solid #000000;width:auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eReagent Name\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003ePreparation\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eVolume (μL)\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eIgG Standard or Test Sample\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:justify;\"\u003e\u003cspan\u003eDilute the compound to the desired concentration using Diluent Buffer according to the reaction system.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eAntibody Mix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:justify;\"\u003e\u003cspan\u003eTake 50 μL of Anti-Human IgG-Eu and dilute to 2500 μL with 1× Detection Buffer, mix well; take 50 μL of Anti-Human IgG-Ac and dilute to 2500 μL with 1× Detection Buffer, mix well, then mix 1:1 to prepare Antibody Mix.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"margin-right:8px;\"\u003e1.2\u003c\/span\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003eStandard or Sample Dilution\u003c\/strong\u003e\u003c\/span\u003e\u003cstrong\u003e:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003eUse 1× Diluent Buffer to prepare the test samples and standards. If the sample stock is in DMSO, it is recommended to keep the DMSO concentration consistent in the system (the Detection Buffer does not contain DMSO; please add DMSO to the 1× Detection Buffer according to the dilution of the standard or test sample), with a maximum of 2%. Prepare the total volume of standards according to experimental needs; the following content is for reference only.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan\u003eTable 3. Serial Dilution of Standards\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:1px solid #000000;width:auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eStandard Preparation Concentration (ng\/mL)\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003ePreparation Method\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e①\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e4000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e5 μL of 40 μg\/mL standard stock solution + 45 μL of 1× Diluent Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e②\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e2000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e25 μL of ① + 25 μL of 1× Diluent Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e③\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e1000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e25 μL of ② + 25 μL of 1× Diluent Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e④\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e500\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e25 μL of ③ + 25 μL of 1× Diluent Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑤\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e250\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e25 μL of ④ + 25 μL of 1× Diluent Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑥\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e125\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e25 μL of ⑤ + 25 μL of 1× Diluent Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑦\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e62.5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e25 μL of ⑥ + 25 μL of 1× Diluent Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑧\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e31.25\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e25 μL of ⑦ + 25 μL of 1× Diluent Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑨\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e15.63\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e25 μL of ⑧ + 25 μL of 1× Diluent Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑩\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e7.81\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e25 μL of ⑨ + 25 μL of 1× Diluent Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003ci\u003e⑪\u003c\/i\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e3.91\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e25 μL of ⑩ + 25 μL of 1× Diluent Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eBlank\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e25 μL of 1× Diluent Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003e*The Blank normally receives Anti-human IgG-Eu and Anti-human IgG-Ac. The NC group receives only Anti-human IgG-Eu, used for calculating\u003c\/span\u003e\u003cspan\u003eNet Signal\u003c\/span\u003e\u003cspan style=\"color:#000000;\"\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003eDilute all test samples with dilution buffer or culture medium. Serial dilutions should be performed within the range of 2000-3.9 ng\/mL (working solution); if the test sample concentration is within the 2000-3.9 ng\/mL range, directly take 10 μL of supernatant for detection.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2. Sample Addition and Controls\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan\u003e2.1 Sample addition order for experimental wells: 10 μL of well-mixed\u003c\/span\u003e\u003cspan style=\"color:#000000;\"\u003eAntibody Mix\u003c\/span\u003e\u003cspan\u003e, 10 μL of standard or test sample, added sequentially into the 384-well shallow plate;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan\u003e2.2 Blank control well (Blank): 1× Detection Buffer;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.3 NC: 15 μL of 1× Detection Buffer plus 5 μL of diluted\u003c\/span\u003e\u003cspan style=\"color:#000000;\"\u003eAnti-Human IgG-Eu.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan\u003eTable 4. Sample Addition and Controls\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:1px solid #000000;width:auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eBlank\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eSample\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eNC\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" rowspan=\"2\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eStep 1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10 μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e1× Detection Buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10 μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eSerially diluted test sample\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" rowspan=\"2\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e15 μL 1× Detection Buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eplus 5 μL 1× Anti-Human IgG-Eu\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"2\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10 μL Antibody Mix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eStep 2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"3\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eSeal the plate wells with a plate sealer\u003c\/span\u003e\u003cstrong\u003e,\u003c\/strong\u003e\u003cspan\u003e and incubate at room temperature for 2 h.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:justify;\"\u003e\u003cspan\u003e*For negative controls and quality control, it is recommended to use a solution with the same matrix as the test sample to replace 1× Detection Buffer.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify;\"\u003e\u003cspan\u003e*Keep DMSO or other matrices consistent across all detection wells.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"margin-right:8px;\"\u003e3.\u003c\/span\u003e\u003cstrong\u003eDetection\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003eSeal the plate wells with a plate sealer, centrifuge at 1000 rpm for 1 min, and incubate at room temperature for 2 h. Detect on a TR-FRET compatible microplate reader (excitation wavelength 320 nm, emission wavelengths 620 nm and 665 nm).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#1655A5;\"\u003e\u003cstrong\u003e[Result Calculation]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify;\"\u003e\u003cspan\u003e1. Calculate the signal value: Divide the 665 nm fluorescence signal by the 620 nm fluorescence signal and multiply by 10000, i.e., Signal Value = (665\/620) * 10000\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan\u003e2. Calculate Net Signal based on the signal value: Net Signal = (Std - NC) \/ NC * 100\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#1655A5;\"\u003e\u003cstrong\u003e[Data Example]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003ePlease note that the following data cannot replace the data obtained in experiments and is provided only as an example. Results may vary depending on the TR-FRET compatible instrument.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"image_resized\" style=\"height:auto;margin-left:30px;vertical-align:middle;width:293px;\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/2b3758af-7c49-4017-a81e-f45706c2c7b7.png\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"image_resized\" style=\"height:auto;margin-left:30px;vertical-align:middle;width:318px;\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/38b2c450-146b-464d-95e0-133fd08d006b.png\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote: Recommended microplate (384-well plate, white, shallow)\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"100T","offer_id":42332756246603,"sku":"UA085006-100T","price":300.0,"currency_code":"USD","in_stock":true},{"title":"500T","offer_id":42332756279371,"sku":"UA085006-500T","price":996.67,"currency_code":"USD","in_stock":true},{"title":"2500T","offer_id":42332756312139,"sku":"UA085006-2500T","price":2951.67,"currency_code":"USD","in_stock":true},{"title":"10000T","offer_id":42332756344907,"sku":"UA085006-10000T","price":5425.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_7584fc87-d03d-4d88-a95a-5ce2ca2e454f.png?v=1788519771"},{"product_id":"unione-tr-fret-human-ddb1-crbnirak4-protac-binding-kit-ua086005","title":"UniOne® TR-FRET Human RAK4\/CRBN PROTAC Binding KIT","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-80℃\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-size:11.0pt;color:black\"\u003eThis kit utilizes Time-Resolved Fluorescence ResonanceEnergy Transfer (TR-FRET) technology to measure the interaction between IRAK4and CRBN mediated by the molecular glue PROTAC IRAK4 DEGRADER-1 and testcompounds. This method provides a simple, rapid, and high-throughput format fordetecting small molecules capable of mediating IRAK4-CRBN interactions.\u003c\/span\u003e\u003cspan style=\"font-size:11.0pt\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:11.0pt;color:black\"\u003eAs shown, the interaction between IRAK4 and CRBN isdetected using an anti-Tag1 antibody labeled with Eu (TR-FRET donor) and ananti-Tag2 antibody labeled with Ac (TR-FRET acceptor). Since the molecular gluePROTAC IRAK4 DEGRADER-1 mediates the interaction between IRAK4 and CRBN, thedonor and acceptor antibodies are brought into proximity. Excitation of thedonor antibody triggers fluorescence resonance energy transfer (FRET) to theacceptor antibody, resulting in specific emission of a 665 nm signal. Thisspecific signal is proportional to the degree of interaction between PROTACIRAK4 DEGRADER-1, IRAK4, and CRBN. This homogeneous assay is simple to performand requires no wash steps.\u003c\/span\u003e\u003cspan style=\"font-size:11.0pt\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg 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\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lang=\"EN-US\"\u003e\u003cstrong\u003e500 TESTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:11.0pt;font-weight:normal;' lang=\"EN-US\"\u003e\u003cstrong\u003e2500 TESTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:11.0pt;font-weight:normal;' lang=\"EN-US\"\u003e\u003cstrong\u003e10000 TESTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:11.0pt;font-weight:normal;' lang=\"EN-US\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003eTag1-IRAK4 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e50×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e8 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e40 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e200 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e800 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003eTag2-DDB1\/CRBN protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e50×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e8 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e40 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e200 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e800 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003ePROTAC IRAK4 degrader-1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e3 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e5 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e25 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e125 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003eAnti-Tag1 Eu dye antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e50×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e10 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e50 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e250 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e1000 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003eAnti-Tag2 Ac antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e12.5×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e40 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e200 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e1000 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e5000 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e10×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e1 mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e2 mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e6 mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e25 mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\u003c\/figure\u003e\u003cp style=\"background-color:white;margin-bottom:8.0pt;text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"background-color:white;color:black;\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e*Avoid repeated freeze-thaw cycles of reagents. Recommend aliquoting after first thaw and storing according to conditions specified for a shelf life of one year.\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"500T","offer_id":42332756836427,"sku":"UA086005-500T","price":2180.0,"currency_code":"USD","in_stock":true},{"title":"2500T","offer_id":42332756869195,"sku":"UA086005-2500T","price":8175.0,"currency_code":"USD","in_stock":true},{"title":"10000T","offer_id":42332756901963,"sku":"UA086005-10000T","price":21800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/09ff6734d3744c5c85bcc84b3c80d9f2.png?v=1788123633"},{"product_id":"unione-tr-fret-human-ddb1-crbngspt1-protac-binding-kit-ua086006","title":"UniOne® TR-FRET Human GSPT1\/CRBN PROTAC Binding Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003eThe kit employs homogeneous time-resolved fluorescence technology (\u003c\/span\u003e\u003cspan\u003eTR-FRET\u003c\/span\u003e\u003cspan\u003e) to measure the interaction between the molecular glue\u003c\/span\u003e\u003cspan\u003eCC-885\u003c\/span\u003e\u003cspan\u003eand the compound under test, mediated by the\u003c\/span\u003e\u003cspan\u003eDDB1\/CRBN\u003c\/span\u003e\u003cspan\u003ecomplex and\u003c\/span\u003e\u003cspan\u003eGSPT1.\u003c\/span\u003e\u003cspan\u003eThis method provides a simple and rapid high-throughput assay for identifying small molecules capable of mediating the interaction between the\u003c\/span\u003e\u003cspan\u003eDDB1\/CRBN\u003c\/span\u003e\u003cspan\u003ecomplex and\u003c\/span\u003e\u003cspan\u003eGSPT1.\u003c\/span\u003e\u003cspan\u003eAs shown in the figure,\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003ethe interaction between\u003c\/span\u003e\u003cspan\u003eDDB1\/CRBN\u003c\/span\u003e\u003cspan\u003eand\u003c\/span\u003e\u003cspan\u003eGSPT1\u003c\/span\u003e\u003cspan\u003eis detected using an\u003c\/span\u003e\u003cspan\u003eEu\u003c\/span\u003e\u003cspan\u003elabeled anti-\u003c\/span\u003e\u003cspan\u003eTag1\u003c\/span\u003e\u003cspan\u003eantibody (\u003c\/span\u003e\u003cspan\u003eTR-FRET\u003c\/span\u003e\u003cspan\u003edonor) and an\u003c\/span\u003e\u003cspan\u003eaccepter\u003c\/span\u003e\u003cspan\u003elabeled anti-\u003c\/span\u003e\u003cspan\u003eTag2\u003c\/span\u003e\u003cspan\u003eantibody (\u003c\/span\u003e\u003cspan\u003eTR-FRET\u003c\/span\u003e\u003cspan\u003eacceptor). Because the molecular glue\u003c\/span\u003e\u003cspan\u003eCC-885\u003c\/span\u003e\u003cspan\u003emediates the interaction between\u003c\/span\u003e\u003cspan\u003eDDB1\/CRBN\u003c\/span\u003e\u003cspan\u003eand\u003c\/span\u003e\u003cspan\u003eGSPT1,\u003c\/span\u003e\u003cspan\u003ethe donor antibody and the acceptor antibody come into close proximity, and excitation of the donor antibody triggers fluorescence resonance energy transfer (\u003c\/span\u003e\u003cspan\u003eFRET\u003c\/span\u003e\u003cspan\u003e) to the acceptor antibody, causing the acceptor antibody to emit fluorescence specifically at\u003c\/span\u003e\u003cspan\u003e665 nm.\u003c\/span\u003e\u003cspan\u003eA signal with a specific wavelength. This particular signal is directly proportional to the extent of interaction between\u003c\/span\u003e\u003cspan\u003eCC-885\u003c\/span\u003e\u003cspan\u003eand\u003c\/span\u003e\u003cspan\u003eDDB1\/CRBN\u003c\/span\u003e\u003cspan\u003e,\u003c\/span\u003e\u003cspan\u003eGSPT1\u003c\/span\u003e\u003cspan\u003e. This homogeneous assay is easy to perform and requires no washing steps.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/f01ccbcb-026a-44a3-b1f1-ddd5c955c484.png\" style=\"margin-left:30px; width:441px; height:auto; vertical-align:middle;\"\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003ctable border=\"1\" style=\"border-collapse:collapse;width:auto;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eComponent\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eConcentration\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e100T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e2500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e10000T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eStorage Temperature\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag1-DDB1\/CRBN protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003e0×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e400\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag2-GSPT1 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003e0×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCC-885\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4mM\u003c\/span\u003e\u003cspan\u003ein\u003c\/span\u003e\u003cspan\u003eDMSO\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e25\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAnti-Tag1 Eu antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003e0\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e250\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003e00\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAnti-Tag2 Ac antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e125\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e500\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e0\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e400μ\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003emL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003emL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e40\u003c\/span\u003e\u003cspan\u003emL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan\u003eNote: After the first thaw, aliquot immediately and store at the recommended temperature. Avoid storing after dilution and repeated freeze-thaw cycles.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e,\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eReagent preparation\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e.1\u003c\/span\u003e\u003cspan\u003eBefore use, allow all reagents to thaw at room temperature (equilibrate at room temperature for at least 30 minutes). The reaction volume in a 384-well plate is 20.\u003c\/span\u003e\u003cspan\u003emu\u003c\/span\u003e\u003cspan\u003eL (the reagent volumes for the reaction system are shown in the table). Calculate the required volume before preparation and prepare accordingly. The following formulation is for reference only, using 500 reactions as an example.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eTable 1\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003cspan\u003eReagent preparation and usage amounts\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eReagent name\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eConfiguration\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eμL per test well\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003etake\u003c\/span\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003em\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003cspan\u003eDetection buffer stock solution,\u003c\/span\u003e\u003cspan\u003eAdd to 18 ml ddH₂O.\u003c\/span\u003e\u003csub\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/sub\u003e\u003cspan\u003eIn O, mix thoroughly to obtain\u003c\/span\u003e\u003cspan\u003e1× Detection buffer\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eStandard substance\u003c\/span\u003e\u003cspan\u003e\/\u003c\/span\u003e\u003cspan\u003eSample to be tested\u003c\/span\u003e\u003cspan\u003eDilution\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eUse diluted to\u003c\/span\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003etimes\u003c\/span\u003e\u003cspan\u003eof\u003c\/span\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003cspan\u003ePrepare the samples to be tested and the standard solutions. If the stock solution of the sample is…\u003c\/span\u003e\u003cspan\u003eDMSO\u003c\/span\u003e\u003cspan\u003e, it is recommended to maintain the system.\u003c\/span\u003e\u003cspan\u003eDMSO\u003c\/span\u003e\u003cspan\u003eThe content is consistent and does not exceed the maximum limit.\u003c\/span\u003e\u003cspan\u003e2%\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003cspan\u003eStandard dilutions are shown in the table below.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag1-DDB1\/CRBN protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003etake\u003c\/span\u003e\u003cspan\u003e20 μL Tag1-DDB1\/CRBN protein\u003c\/span\u003e\u003cspan\u003eUndiluted solution,\u003c\/span\u003e\u003cspan\u003e1× Detection buffer\u003c\/span\u003e\u003cspan\u003eDilute to\u003c\/span\u003e\u003cspan\u003e2 mL,\u003c\/span\u003e\u003cspan\u003eMix well and set aside.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag2-GSPT1 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003etake\u003c\/span\u003e\u003cspan\u003e20 μL Tag2-GSPT1 protein\u003c\/span\u003e\u003cspan\u003eUndiluted solution,\u003c\/span\u003e\u003cspan\u003eDilute 1× detection buffer to a final volume of 2 mL.\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eMix well and set aside.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAntibody\u003c\/span\u003e\u003cspan\u003eMix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003etake\u003c\/span\u003e\u003cspan\u003eFifty\u003c\/span\u003e\u003cspan\u003eμL Anti-Tag1 Eu antibody\u003c\/span\u003e\u003cspan\u003eConcentrate, add\u003c\/span\u003e\u003cspan\u003e1× Detection buffer 2.45 mL,\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e \u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eSufficient\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eMix well\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e;\u003c\/span\u003e\u003cspan\u003etake\u003c\/span\u003e\u003cspan\u003e25 μL Anti-Tag2 Ac antibody\u003c\/span\u003e\u003cspan\u003eConcentrate, add\u003c\/span\u003e\u003cspan\u003e1× Detection buffer 2.4\u003c\/span\u003e\u003cspan\u003eseventy-five\u003c\/span\u003e\u003cspan\u003emL,\u003c\/span\u003e\u003cspan\u003eMix thoroughly; combine the above two components in a 1:1 ratio to obtain the Antibody Mix.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan\u003e1.2\u003c\/span\u003e\u003cspan\u003eStandard Preparation Table\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003ePlease prepare the total amount of standard solutions based on the experimental conditions; the following information is for reference only.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eUse diluted to\u003c\/span\u003e\u003cspan\u003e1X\u003c\/span\u003e\u003cspan\u003ePrepare the samples to be tested and the standards using the assay buffer. If the stock solution of the sample is…\u003c\/span\u003e\u003cspan\u003eDMSO\u003c\/span\u003e\u003cspan\u003e, it is recommended to maintain within the system.\u003c\/span\u003e\u003cspan\u003eDMSO\u003c\/span\u003e\u003cspan\u003eConsistent content,\u003c\/span\u003e\u003cspan\u003e(\u003c\/span\u003e\u003cspan\u003eThe detection buffer does not contain\u003c\/span\u003e\u003cspan\u003eDMSO\u003c\/span\u003e\u003cspan\u003ePlease, based on the dilution of the standard or the sample to be tested, proceed to…\u003c\/span\u003e\u003cspan\u003e1X\u003c\/span\u003e\u003cspan\u003eAdded to the detection buffer\u003c\/span\u003e\u003cspan\u003eDMSO)\u003c\/span\u003e\u003cspan\u003eand not exceeding a maximum of\u003c\/span\u003e\u003cspan\u003e2%\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eTable\u003c\/span\u003e\u003cspan\u003e2.\u003c\/span\u003e\u003cspan\u003eStandard Preparation Table\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eCC-885\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eFinal concentration (\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003enM\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eCC-885\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003ePreparation concentration (nM)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eConfiguration method\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4000.00\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e40000.0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1 μL 4 mM\u003c\/span\u003e\u003cspan\u003eMother liquor\u003c\/span\u003e\u003cspan\u003e+99 μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1333.33\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e13333.33\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e30 μL\u003c\/span\u003e\u003cspan\u003e①\u003c\/span\u003e\u003cspan\u003e+60 μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e3\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e444.44\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4444.44\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e30 μL\u003c\/span\u003e\u003cspan\u003e②\u003c\/span\u003e\u003cspan\u003e+60 μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e148.15\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1481.48\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e30 μL\u003c\/span\u003e\u003cspan\u003e③\u003c\/span\u003e\u003cspan\u003e+60 μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e49.38\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e493.83\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e30 μL\u003c\/span\u003e\u003cspan\u003e④\u003c\/span\u003e\u003cspan\u003e+60 μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e6\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e16.46\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e164.61\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e30 μL\u003c\/span\u003e\u003cspan\u003e⑤\u003c\/span\u003e\u003cspan\u003e+60 μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e7\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5.49\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e54.87\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e30 μL\u003c\/span\u003e\u003cspan\u003e⑥\u003c\/span\u003e\u003cspan\u003e+60 μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e8\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1.83\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e18.29\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e30 μL\u003c\/span\u003e\u003cspan\u003e⑥\u003c\/span\u003e\u003cspan\u003e+60 μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e9\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e0.61\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e6.1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e30 μL\u003c\/span\u003e\u003cspan\u003e⑥\u003c\/span\u003e\u003cspan\u003e+60 μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eBlank\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e60 μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e2.\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eSample addition and controls\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.1 Sample to be tested: Add 2 μL of the gradient-diluted sample, 4 μL of Tag1-DDB1\/CRBN protein working solution, and 4 μL of Tag2-GSPT1 protein into a 384-well plate in sequence.\u003c\/span\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003cspan\u003eAntibody\u003c\/span\u003e\u003cspan\u003eMix\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.2 Positive control standard curve: Add 2 μL of CC-885 diluted in a gradient, 4 μL of Tag1-DDB1\/CRBN protein working solution, 4 μL of Tag2-GSPT1 protein,\u003c\/span\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003cspan\u003eAntibody\u003c\/span\u003e\u003cspan\u003eMix\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e.3\u003c\/span\u003e\u003cspan\u003eNegative control well (NC): Replace the sample to be tested with 2 μL of 1× Detection buffer;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.4 Eu reference well: Add 10 μL of 1× Detection buffer plus\u003c\/span\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003cspan\u003eAntibody\u003c\/span\u003e\u003cspan\u003eMix\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse:collapse;width:auto;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003ePositive control standard curve\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eSample to be tested\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNegative control well (NC)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eEu reference well\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"4\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eStep one\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eGradient dilution\u003c\/span\u003e\u003cspan\u003eCC-885\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eGradient dilute the sample to be tested\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"5\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10μL 1\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e10μL Antibody Mix\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eTag1-DDB1\/CRBN protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4μL Tag2- GSPT1 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eSeal the plate,\u003c\/span\u003e\u003cspan\u003e1000rpm\u003c\/span\u003e\u003cspan\u003e, centrifuge\u003c\/span\u003e\u003cspan\u003e1min\u003c\/span\u003e\u003cspan\u003eMix well,\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eincubate at room temperature\u003c\/span\u003e\u003cspan\u003e10min\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"2\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eStep two\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10μL Antibody Mix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"4\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eSeal the plate,\u003c\/span\u003e\u003cspan\u003e1000rpm\u003c\/span\u003e\u003cspan\u003e, centrifuge\u003c\/span\u003e\u003cspan\u003e1min\u003c\/span\u003e\u003cspan\u003eMix well,\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eincubate at room temperature\u003c\/span\u003e\u003cspan\u003e120min\u003c\/span\u003e\u003cspan\u003e, read the plate\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e3,\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003edetection\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eDetect on a TR-FRET-compatible microplate reader\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003cspan\u003eExcitation light is 320\/340nm, detecting emission wavelengths at 620nm and 665nm.\u003c\/span\u003e\u003cspan\u003eEmission\u003c\/span\u003e\u003cspan\u003ewavelengths.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e[Result Calculation]\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1)\u003c\/span\u003e\u003cspan\u003eCalculate the signal value\u003c\/span\u003e\u003cspan\u003e(Ratio)\u003c\/span\u003e\u003cspan\u003e: The 665nm fluorescence signal is multiplied by 10,000 compared to the 620nm fluorescence signal.\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eRatio\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e=\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e(665\/620)\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003cspan\u003e10000\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2)\u003c\/span\u003e\u003cspan\u003eCalculate based on the signal value.\u003c\/span\u003e\u003cspan\u003eNet signal\u003c\/span\u003e\u003cspan\u003e:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eNet signal\u003c\/span\u003e\u003cspan\u003e= (Std-Blank)\/Blank × 100\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3)\u003c\/span\u003e\u003cspan\u003eCalculate CV (%).\u003c\/span\u003e\u003cspan\u003e:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eCV (%) = Standard Deviation \/ Mean Ratio × 100%\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e[Data Example]\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe following data cannot replace the data obtained in experiments; it is only for illustrative purposes, and the results may\u003c\/span\u003e\u003cspan\u003evary depending on the plate reader.\u003c\/span\u003e\u003cspan\u003eInstrument\u003c\/span\u003e\u003cspan\u003emay vary.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e(1) Standard Curve*\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe following data cannot replace the data obtained in experiments; it is only for illustrative purposes, and the results may vary depending on\u003c\/span\u003e\u003cspan\u003eTR-FRET\u003c\/span\u003e\u003cspan\u003ecompatible instruments.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#FFFFFF\"\u003e \u003c\/span\u003e\u003cspan style=\"color:#FFFFFF\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/5ca31b5e-ff97-4d7e-96de-6d62acab7f50.png\" style=\"margin-left:30px; width:480px; height:auto; vertical-align:middle;\"\u003e\u003c\/p\u003e\n\u003cspan style=\"color:#FFFFFF\"\u003e \u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e*\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eData are provided for illustrative reference only; a microplate is recommended\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e(384-well plate, white, shallow wells).\u003c\/span\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"100T","offer_id":43184007708747,"sku":"UA086006-100T","price":730.0,"currency_code":"USD","in_stock":true},{"title":"500T","offer_id":42332756934731,"sku":"UA086006-500T","price":2180.0,"currency_code":"USD","in_stock":true},{"title":"2500T","offer_id":42332756967499,"sku":"UA086006-2500T","price":8175.0,"currency_code":"USD","in_stock":true},{"title":"10000T","offer_id":42332757000267,"sku":"UA086006-10000T","price":21800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_be70ef2a-c8ee-4eb0-a048-ccfe92354305.png?v=1789621350"},{"product_id":"unione-tr-fret-human-tnf-tnfr1-binding-kit-ua086007","title":"UniOne® TR-FRET Human TNF-α TNFR1 Binding Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-80℃\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003eThe kit utilizes homogeneous time-resolved fluorescence technology (TR-FRET) to measure the interaction between TNF-α and TNFR1. This method enables simple and rapid high-throughput screening of inhibitors and antibody blockers.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAs shown in the figure, the interaction between TNF-α and TNFR1 is detected using a Eu-labeled anti-Tag1 antibody (TR-FRET donor) and an Ac-labeled anti-Tag2 antibody (TR-FRET acceptor). The binding of TNF-α to TNFR1 brings the donor and acceptor antibodies into close proximity, allowing fluorescence resonance energy transfer (FRET) from the donor to the acceptor, resulting in a specific emission signal at 665 nm.\u003c\/span\u003e\u003cspan\u003e The positive control drug Adalimumab blocks the binding of TNF-α to TNFR1, preventing FRET signal generation. The stronger the inhibitory effect of the screened drug on the TNF-α\/TNFR1 interaction, the lower the signal.\u003c\/span\u003e\u003cspan\u003e This specific signal is proportional to the extent of TNF-α\/TNFR1 interaction. The homogeneous assay is simple to perform and requires no washing steps.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/ed3f7e22-b0a2-46dc-b580-565e2c90ec7e.png\" style=\"margin-left:30px; width:373px; height:auto; vertical-align:middle;\" alt=\"\"\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003ctable border=\"1\" style=\"border-collapse:collapse;width:auto;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eComponent\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eConcentration\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e100T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e2500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e10000T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eStorage Temperature\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag1-TNF-α protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100 ×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag2- TNFR1 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100 ×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eEu-anti-Tag1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50 ×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e250μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1000μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAc-anti-Tag2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e12.5 ×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e40μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e200μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10 ×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e40mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"color:#000000\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"color:#000000\"\u003eNote: Aliquot immediately after initial thawing and store at recommended temperatures. Avoid storage after dilution and repeated freeze-thaw cycles.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003eHere is the English translation while preserving the original formatting:```html\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003e. \u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003eReagent Preparation\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003e1.1 \u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eThaw all reagents at room temperature before use (equilibrate for at least 30 min). The reaction system in 384-well plates is 20\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eμ\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eL (reagent volumes are shown in the table below). Calculate the required volume before preparation. The following preparation is for reference only, using 500 reactions as an example.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"color:#000000\"\u003eTable 1\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e. \u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eReagent Preparation\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eReagent Name\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003ePreparation\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eVolume per Well (μL)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan\u003eAdd 2\u003c\/span\u003e\u003cspan\u003emL of 10× Detection buffer to 18 mL deionized water, dilute to 1×, and mix well.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag1-TNF-α protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan\u003eAdd 20μL of 100× Tag1-TNFα protein stock to 1.98 mL 1× Detection buffer, dilute to 1×, and mix well.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag2-TNFR1 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan\u003eAdd 20μL of 100× Tag2-TNFR1 protein stock to 1.98 mL 1× Detection buffer, dilute to 1×, and mix well.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDetection Mix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp\u003e\u003cspan\u003eAdd 50\u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan\u003eL of 50× Eu-anti-Tag1 stock to 2\u003c\/span\u003e\u003cspan\u003e.4\u003c\/span\u003e\u003cspan\u003e5 mL 1× Detection buffer, dilute to 2.5 mL, and mix well;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAdd 200μL of 12.5× Ac-anti-Tag2 stock to 2.30 mL 1× Detection buffer, dilute to 2.5 mL, and mix well;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eMix the two solutions at 1:1 ratio to prepare the Detection Mix.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan style=\"color:#000000\"\u003e1.2 \u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eSample serial dilution\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eTake \u003c\/span\u003e\u003cspan\u003eAdalimumab\u003c\/span\u003e\u003cspan\u003e as an example. The diluent is 1× Detection buffer. To minimize matrix effects, it is recommended to use a solution with the same matrix as the sample. Adjust the dilution according to the actual sample concentration.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"color:#000000\"\u003eTable 2. \u003c\/span\u003e\u003cspan\u003eAdalimumab serial dilution (adjust as needed)\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eAdalimumab Final Conc. (nM)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eAdalimumab Prep. Conc. (nM)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003ePreparation Method\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e①\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1000.00 \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10000.00 \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003eμL \u003c\/span\u003e\u003cspan\u003e375\u003c\/span\u003e\u003cspan\u003eμM stock + \u003c\/span\u003e\u003cspan\u003e36.5\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e②\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e400.000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4000.000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003eμL \u003c\/span\u003e\u003cspan\u003e①\u003c\/span\u003e\u003cspan\u003e +\u003c\/span\u003e\u003cspan\u003e15\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e③\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e160.000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1600.000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003eμL \u003c\/span\u003e\u003cspan\u003e②\u003c\/span\u003e\u003cspan\u003e +\u003c\/span\u003e\u003cspan\u003e15\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e④\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e64.000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e640.000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003eμL \u003c\/span\u003e\u003cspan\u003e③\u003c\/span\u003e\u003cspan\u003e +\u003c\/span\u003e\u003cspan\u003e15\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑤\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e25.600\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e256.000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003eμL \u003c\/span\u003e\u003cspan\u003e④\u003c\/span\u003e\u003cspan\u003e +\u003c\/span\u003e\u003cspan\u003e15\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑥\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10.240\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e102.400\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003eμL \u003c\/span\u003e\u003cspan\u003e⑤\u003c\/span\u003e\u003cspan\u003e +\u003c\/span\u003e\u003cspan\u003e15\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑦\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4.096\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e40.960\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003eμL \u003c\/span\u003e\u003cspan\u003e⑥\u003c\/span\u003e\u003cspan\u003e +\u003c\/span\u003e\u003cspan\u003e15\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑧\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1.638\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e16.384\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003eμL \u003c\/span\u003e\u003cspan\u003e⑦\u003c\/span\u003e\u003cspan\u003e +\u003c\/span\u003e\u003cspan\u003e15\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑨\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e0.655\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e6.554\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003eμL \u003c\/span\u003e\u003cspan\u003e⑧\u003c\/span\u003e\u003cspan\u003e +\u003c\/span\u003e\u003cspan\u003e15\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑩\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e0.262\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2.621\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003eμL \u003c\/span\u003e\u003cspan\u003e⑨\u003c\/span\u003e\u003cspan\u003e +\u003c\/span\u003e\u003cspan\u003e15\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑪\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e0.105\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1.049\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003eμL \u003c\/span\u003e\u003cspan\u003e⑩\u003c\/span\u003e\u003cspan\u003e +\u003c\/span\u003e\u003cspan\u003e15\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eBlank\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e15\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003e2\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003e. \u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003eSample Loading and Controls\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.1 \u003c\/span\u003e\u003cspan\u003eSample wells: Add 2μL of sample (serial dilution), 4μL Tag1-TNF-α protein working solution, 4μL Tag2-TNFR1 protein working solution, and 10μL Detection Mix sequentially to 384-well plates.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.2 \u003c\/span\u003e\u003cspan\u003eMax signal control: Add 2μL Detection buffer, 4μL Tag1-TNF-α protein working solution, 4μL Tag2-TNFR1 protein working solution, and 10μL Detection Mix sequentially to 384-well plates.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.3 \u003c\/span\u003e\u003cspan\u003eNegative control (NC): Add 10μL Detection buffer and 10μL Detection Mix sequentially to 384-well plates.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAfter loading all samples, centrifuge, seal with plate seal, and incubate at room temperature for 2 hours.\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse:collapse;width:auto;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e　\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eSample\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eMax Signal Control\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNegative Control (NC)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eStep 1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2μL sample\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2μL 1×Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd rowspan=\"4\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10μL 1×Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"2\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4μL Tag1-TNF-α protein working solution\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"2\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate at RT for 10 min\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eStep 2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd colspan=\"2\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4μL Tag2-TNFR1 protein working solution\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10μL Detection Mix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eSeal plate and incubate at RT for 2h\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e3. \u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003eDetection\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003eRead plates using a TR-FRET compatible microplate reader. Excitation wavelength: 320\/340 nm; emission wavelengths: 620 nm and 665 nm. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e[Data Analysis]\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003e1)\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e Calculate signal ratio: Divide 665 nm fluorescence signal by 620 nm fluorescence signal and multiply by 10000.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"color:#000000\"\u003eRatio\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e \u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e=\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e \u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e(665\/620)\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e ×\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e10000\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003e2)\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e Calculate Net signal based on ratio: \u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"color:#000000\"\u003eNet signal\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e = (Std-\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eNC\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e)\/\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eNC\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e×100\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003e3)\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e Calculate CV (%):\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"color:#000000\"\u003eCV (%) = Standard Deviation\/Mean Ratio × 100%\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e[Example Data]\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003eThe following data is for reference only and may vary depending on the plate reader used.\u003c\/span\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/2e04f06b-536a-4283-92a8-c805188355c3.png\" style=\"margin-left:30px; width:450px; height:auto; vertical-align:middle;\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003eNote: Recommended microplate (384-well plate, white, shallow well)\u003c\/span\u003e\u003c\/p\u003e```\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"500T","offer_id":42332757033035,"sku":"UA086007-500T","price":2180.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":43090348736587,"sku":"UA086007-100T","price":730.0,"currency_code":"USD","in_stock":true},{"title":"2500T","offer_id":42332757065803,"sku":"UA086007-2500T","price":8175.0,"currency_code":"USD","in_stock":true},{"title":"10000T","offer_id":42332757098571,"sku":"UA086007-10000T","price":21800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_b60baccc-8934-43ee-b312-d3e7d9156f50.png?v=1788519774"},{"product_id":"unione-tr-fret-human-tnf-tnfr2-binding-kit-ua086008","title":"UniOne® TR-FRET Human TNF-α\/TNFR2 Binding Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-80℃\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-size:11.0pt;color:black\"\u003eThis kit employsHomogeneous Time-Resolved Fluorescence (TR-FRET) technology to measure the interactionbetween TNF-α and TNFR2. The method enables simple and rapid high-throughputscreening of inhibitors and antibody blockers.\u003c\/span\u003e\u003cspan style=\"font-size:11.0pt\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:11.0pt;color:black\"\u003eAs shown in thefigure, the TNF-α\/TNFR2 interaction is detected using an Eu-labeled anti-Tag1antibody (TR-FRET donor) and an Acceptor-labeled TNF-α protein (TR-FRETacceptor). Binding of TNF-α to TNFR2 brings the donor antibody into proximitywith the acceptor protein. Excitation of the donor antibody triggersFluorescence Resonance Energy Transfer (FRET) to the acceptor, resulting inspecific emission at 665 nm. This specific signal is proportional to the levelof TNF-α\/TNFR2 interaction. This homogeneous assay is simple to perform andrequires no wash steps.\u003c\/span\u003e\u003cspan style=\"font-size:11.0pt\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/product.template\/20289050\/d56942e8-3b74-4090-a93e-d1e49944b314.png?access_token=363abac1-8e97-4f7b-b34e-5d6afc6d8a1e\" class=\"img img-fluid o_we_custom_image\" style=\"width: 50%;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:none;\" border=\"1\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003cthead\u003e\u003ctr\u003e\n\u003cth style=\"border:solid windowtext 1.0pt;padding:7.5pt 7.5pt 7.5pt 7.5pt;\" valign=\"bottom\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 7.5pt 7.5pt 7.5pt;\" valign=\"bottom\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 7.5pt 7.5pt 7.5pt;\" valign=\"bottom\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e500 TESTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 7.5pt 7.5pt 7.5pt;\" valign=\"bottom\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e2500 TESTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 7.5pt 7.5pt 7.5pt;\" valign=\"bottom\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e10000 TESTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 7.5pt 7.5pt 7.5pt;\" valign=\"bottom\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003eStorage Temperature\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 7.5pt 7.5pt 7.5pt;\" valign=\"bottom\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eTNF\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eΑ\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-01A protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 7.5pt 7.5pt 7.5pt;\" valign=\"bottom\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 7.5pt 7.5pt 7.5pt;\" valign=\"bottom\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50 \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eμ\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 7.5pt 7.5pt 7.5pt;\" valign=\"bottom\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e250 \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eμ\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 7.5pt 7.5pt 7.5pt;\" valign=\"bottom\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e500 \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eμ\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 7.5pt 7.5pt 7.5pt;\" valign=\"bottom\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e°\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eC\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 7.5pt 7.5pt 7.5pt;\" valign=\"bottom\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eTag1-TNFR2 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 7.5pt 7.5pt 7.5pt;\" valign=\"bottom\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e100\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 7.5pt 7.5pt 7.5pt;\" valign=\"bottom\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e40 \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eμ\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 7.5pt 7.5pt 7.5pt;\" valign=\"bottom\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e200 \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eμ\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 7.5pt 7.5pt 7.5pt;\" valign=\"bottom\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e800 \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eμ\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 7.5pt 7.5pt 7.5pt;\" valign=\"bottom\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e°\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eC\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 7.5pt 7.5pt 7.5pt;\" valign=\"bottom\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eAnti-Tag1 Eu-dye antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 7.5pt 7.5pt 7.5pt;\" valign=\"bottom\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 7.5pt 7.5pt 7.5pt;\" valign=\"bottom\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50 \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eμ\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 7.5pt 7.5pt 7.5pt;\" valign=\"bottom\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e250 \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eμ\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 7.5pt 7.5pt 7.5pt;\" valign=\"bottom\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1000 \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eμ\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 7.5pt 7.5pt 7.5pt;\" valign=\"bottom\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e°\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eC\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 7.5pt 7.5pt 7.5pt;\" valign=\"bottom\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 7.5pt 7.5pt 7.5pt;\" valign=\"bottom\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 7.5pt 7.5pt 7.5pt;\" valign=\"bottom\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2 mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 7.5pt 7.5pt 7.5pt;\" valign=\"bottom\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10 mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 7.5pt 7.5pt 7.5pt;\" valign=\"bottom\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e40 mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 7.5pt 7.5pt 7.5pt;\" valign=\"bottom\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e°\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eC\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cspan style=\"background-color:rgb(255,255,255);color:rgb(0,0,0);\"\u003e\u003cspan style=\"-webkit-text-stroke-width:0px;display:inline !important;float:none;font-family:宋体;font-size:14.6667px;font-style:normal;font-variant-caps:normal;font-variant-ligatures:normal;font-weight:400;letter-spacing:normal;orphans:2;text-align:left;text-decoration-color:initial;text-decoration-style:initial;text-decoration-thickness:initial;text-indent:0px;text-transform:none;white-space:normal;widows:2;word-spacing:0px;\"\u003e* Avoid repeated freeze-thaw cycles. Aliquot after first thaw and store according to conditions indicated. Shelf life is one year.\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e1\u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003eReagent Preparation\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;text-autospace:none;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eThaw all reagents to room temperature before use (equilibrate at room temperature for at least\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e30min\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e).\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e384\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e-well low-volume plate reaction volume is\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e20μL\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e (reagent volumes for the reaction system are shown in the table). Calculate the required volume for the experiment before preparation and prepare as needed; the following preparation is for reference only, using\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e500\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003ereactions as an example.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e1.1 \u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003ePreparation of Buffer (\u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e1\u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e×\u003cstrong\u003e)\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"Default\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;font-weight:normal;\" lang=\"EN-US\"\u003e\u003cstrong\u003e2mL\u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e Detection buffer \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e(\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e×)\u003c\/span\u003e\u003cspan style=\"color:windowtext;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eadd\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e18mL\u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;font-weight:normal;\"\u003e\u003cstrong\u003esterile water, mix well and set aside;\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"Default\"\u003e \u003c\/p\u003e\n\u003cp class=\"Default\" style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eTable\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2. \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eReagent Preparation and Volumes\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:none;margin-left:10.15pt;\" border=\"1\" cellspacing=\"0\" cellpadding=\"0\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:114.2pt;\" width=\"152\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003eReagent Name\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:232.85pt;\" width=\"310\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003ePreparation\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:61.7pt;\" width=\"82\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003eVolume (\u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003eμL\u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003e)\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:114.2pt;\" width=\"152\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eTest Sample\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:232.85pt;\" width=\"310\" valign=\"top\"\u003e\n\u003cp\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eDilute the test sample to the desired concentration using\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e×\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e according to the reaction system.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eIf\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDMSO\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eis used, ensure the\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDMSO\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003econcentration is consistent across all detection wells.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:61.7pt;\" width=\"82\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:114.2pt;\" width=\"152\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eTNFΑ-01A protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:232.85pt;\" width=\"310\" valign=\"top\"\u003e\n\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eTake\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50μL TNFΑ-01A protein\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003estock solution,\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e×\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003edilute to\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2.5mL ,\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;font-weight:normal;\"\u003e\u003cstrong\u003emix well and set aside.\u003c\/strong\u003e\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:61.7pt;\" width=\"82\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:114.2pt;\" width=\"152\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eTag1- TNFR2 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:232.85pt;\" width=\"310\" valign=\"top\"\u003e\n\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eTake\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e40μL Tag1- TNFR2 protein\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003estock solution,\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e×\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003edilute to\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e4mL ,\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;font-weight:normal;\"\u003e\u003cstrong\u003emix well and set aside.\u003c\/strong\u003e\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:61.7pt;\" width=\"82\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e8\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:114.2pt;\" width=\"152\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eAnti-Tag1 Eu dye antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:232.85pt;\" width=\"310\" valign=\"top\"\u003e\n\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eTake\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50μL Anti-Tag1 Eu dye antibody\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e, using\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e×\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003edilute to\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2500μL\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e, mix well and set aside.\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:61.7pt;\" width=\"82\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp class=\"Default\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e1.2 \u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003eSerial Dilution of Test Samples\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;text-indent:21.0pt;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003ePrepare test samples using\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e×\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e. If the sample stock is in\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDMSO\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e, it is recommended to maintain a consistent\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDMSO \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003econcentration in the system,\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e(\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003ethe detection buffer does not contain\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDMSO\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e; please add\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDMSO\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eto the\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e×\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eaccording to the dilution of the standard or test sample)\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e, with a maximum concentration not exceeding\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2%\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e2 \u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003eSample Addition and Controls\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;text-autospace:none;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2.1 \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eAddition order for experimental wells:\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2μL \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003etest sample,\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e8μL Tag1- TNFR2 protein\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eworking solution,\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e5μL TNFΑ-01A protein protein\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e,\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e5μL Anti-Tag1 Eu dye antibody\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e, sequentially added into the\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e384\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e-well low-volume plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;text-autospace:none;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2.2 \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eMinimum control well:\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10μL 1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e× \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e (containing\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDMSO\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e),\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e5μL TNFΑ-01A protein\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e,\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e5μL Anti-Tag1 Eu dye antibody\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;text-autospace:none;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2.3 \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eMaximum control well:\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2μL 1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e× \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e (containing\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDMSO\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e),\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e8μL Tag1- TNFR2 protein\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e,\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e5μL TNFΑ-01A protein\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e,\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e5μL Anti-Tag1 Eu dye antibody\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2.4 Eu only\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e:\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e15μL 1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e× buffer plus\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e5μL\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003ediluted\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eAnti-Tag1 Eu dye antibody\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eTable\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e4. \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eSample Addition and Controls\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:none;\" border=\"1\" cellspacing=\"0\" cellpadding=\"0\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:44.0pt;\" width=\"59\" valign=\"top\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:96.8pt;\" width=\"129\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eMinimum Control\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:115.75pt;\" width=\"154\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eMaximum Control\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:102.05pt;\" width=\"136\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eSample\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:67.5pt;\" width=\"90\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eEu Only\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:44.0pt;\" rowspan=\"2\" width=\"59\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eStep 1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:96.8pt;\" width=\"129\" valign=\"top\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e× \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e (containing\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDMSO\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:115.75pt;\" width=\"154\" valign=\"top\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e× \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e (containing\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDMSO\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:102.05pt;\" width=\"136\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eSerially diluted test sample\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:67.5pt;\" rowspan=\"3\" width=\"90\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e15uL 1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e× \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:19.25pt;\"\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:19.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:96.8pt;\" width=\"129\" valign=\"top\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e8μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e× \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e (containing\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDMSO\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:19.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:217.8pt;\" colspan=\"2\" width=\"290\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e8μL Tag1- TNFR2 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:44.0pt;\" rowspan=\"2\" width=\"59\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eStep 2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:314.6pt;\" colspan=\"3\" width=\"419\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e5μL TNFΑ-01A protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:382.1pt;\" colspan=\"4\" width=\"509\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e5μL Anti-Tag1 Eu dye antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:44.0pt;\" width=\"59\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eStep 3\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:382.1pt;\" colspan=\"4\" width=\"509\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eSeal the plate with a sealing film\u003cstrong\u003e,\u003c\/strong\u003e and incubate at room temperature for\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2h\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e*\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eFor negative controls and quality control, it is recommended to use\u003c\/span\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003ea solution with the same matrix as the test sample to replace\u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e×\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e.\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e*\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eEnsure consistent\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDMSO\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eor other matrix concentrations across all detection wells.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e3.\u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003eDetection\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;text-autospace:none;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eDetect using a microplate reader compatible with\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eTR-FRET\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e (excitation wavelength at\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e320nm\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e, emission wavelengths at\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e620nm\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e and\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e665nm\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;text-autospace:none;\" align=\"left\"\u003e \u003c\/p\u003e\n\u003cp style=\"background-color:white;line-height:150%;margin:0cm 9.0pt 0cm 0cm;\"\u003e\u003cspan style=\"color:#7030A0;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;line-height:150%;\"\u003e\u003cstrong\u003e[Result Calculation]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;text-autospace:none;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1. \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eCalculate signal value:\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e665nm\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003efluorescence signal divided by\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e620nm\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003efluorescence signal, then multiplied by\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10000\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e, i.e.,\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;text-autospace:none;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eSignal Value\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e= (665\/620)*10000\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;text-autospace:none;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2. \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eCalculate the δ value based on the signal value:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;text-autospace:none;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eδ Value\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e= (Std-NC)\/NC*100\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;text-autospace:none;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e3.\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eCalculate\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eCV\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e (\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e%\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;text-autospace:none;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eCV\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e (\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e%\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e)\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e= Standard Deviation\/Mean Ratio\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e×\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e100%\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"background-color:white;line-height:150%;margin:0cm 9.0pt 0cm 0cm;\"\u003e\u003cspan style=\"color:#7030A0;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;line-height:150%;\"\u003e\u003cstrong\u003e[Data Example]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eThe following data cannot replace data obtained from actual experiments and is provided for illustration only. Results may vary depending on the\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eTR-FRET\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003ecompatible instrument.\u003c\/span\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"500T","offer_id":42332757131339,"sku":"UA086008-500T","price":2180.0,"currency_code":"USD","in_stock":true},{"title":"2500T","offer_id":42332757164107,"sku":"UA086008-2500T","price":8175.0,"currency_code":"USD","in_stock":true},{"title":"10000T","offer_id":42332757196875,"sku":"UA086008-10000T","price":21800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/554b829102e244258451bea2c91ca38e.png?v=1788123757"},{"product_id":"unione-tr-fret-human-brd4crbn-protac-binding-kit-ua086009","title":"UniOne® TR-FRET Human BRD4\/CRBN PROTAC Binding Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-80℃\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-size:11.0pt;color:#0F1115\"\u003eThis kit utilizes Homogeneous Time-Resolved Fluorescence(TR-FRET) technology to measure the Molecular Glue BET Degrader-1 and testcompound-mediated interaction between the DDB1\/CRBN complex and BRD4. BRD4contains bromodomain 1 and 2. This method enables a simple and rapidhigh-throughput format for detecting small molecules capable of mediating theinteraction between the DDB1\/CRBN complex and BRD4.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:11.0pt;color:#0F1115\"\u003eAs shown, the interaction between DDB1\/CRBN and BRD4 isdetected using a Eu-labeled Anti-Tag1 Antibody (TR-FRET donor) and anAcceptor-labeled Anti-Tag2 Antibody (TR-FRET acceptor). As the Molecular GlueBET Degrader-1 mediates the interaction between DDB1\/CRBN and BRD4, the donorand acceptor antibodies are brought into proximity. Excitation of the donor antibodytriggers Fluorescence Resonance Energy Transfer (FRET) to the acceptorantibody, resulting in a specific emission signal at 665nm. This specificsignal is proportional to the degree of interaction mediated by BET Degrader-1between DDB1\/CRBN and BRD4. This homogeneous assay is simple to perform andrequires no washing steps.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/product.template\/20289075\/bce87b3c-36fe-4f6f-a172-3262c154d777.png?access_token=ccc20f25-7f3b-4ebe-8eb2-1a373a3d129d\" class=\"img img-fluid o_we_custom_image\" style=\"width: 50%;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:none;\" border=\"1\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003cthead\u003e\u003ctr\u003e\n\u003cth style=\"border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e500 TESTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e2500 TESTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e10000 TESTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eTag1-DDB1\/CRBN protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e25×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e80μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1600μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eTag2-BRD4 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e20μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e100μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eBET Degrader-1 (100μM)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10μM\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e200μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eAnti-Tag1 Eu dye antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e250μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1000μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eAnti-Tag2 Ac antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e12.5×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e200μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1000μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e4000μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e40mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\u003c\/figure\u003e\u003cp style=\"background-color:white;margin:12.0pt 0cm;text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:#0F1115;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e*Repeated freeze-thaw cycles should be avoided. Aliquot upon first thaw and store according to the specified conditions. Valid for one year.\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"100T","offer_id":43090353160267,"sku":"UA086009-100T","price":730.0,"currency_code":"USD","in_stock":true},{"title":"500T","offer_id":42332757229643,"sku":"UA086009-500T","price":2180.0,"currency_code":"USD","in_stock":true},{"title":"2500T","offer_id":42332757262411,"sku":"UA086009-2500T","price":8175.0,"currency_code":"USD","in_stock":true},{"title":"10000T","offer_id":42332757295179,"sku":"UA086009-10000T","price":21800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/61d1e2c39c194f5f868ca8aa8376fcb7.png?v=1788123761"},{"product_id":"unione-tr-fret-human-il2il2r-binding-kit-ua086010","title":"UniOne® TR-FRET Human IL2\/IL2R Binding Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-80℃\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-size:11.0pt\"\u003eThis kit utilizes HomogeneousTime-Resolved Fluorescence (TR-FRET) technology to measure the interactionbetween IL2 and IL2R. This method enables simple and rapid high-throughputscreening of inhibitors and blocking antibodies.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:11.0pt\"\u003eAs shown, the interaction betweenIL2 and IL2R is detected using a Eu-labeled Anti-Tag1 Antibody (TR-FRET donor)and an Acceptor-labeled Anti-Tag2 Antibody (TR-FRET acceptor). The binding ofIL2 to IL2R brings the donor and acceptor antibodies into proximity. Excitationof the donor antibody triggers Fluorescence Resonance Energy Transfer (FRET) tothe acceptor antibody, resulting in a specific emission signal at 665nm. Thisspecific signal is proportional to the degree of interaction between IL2 andIL2R. This homogeneous assay is simple to perform and requires no washingsteps.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/product.template\/20289090\/60a97f5e-3025-41a5-ac48-c80b44b68564.png?access_token=fb1b24b4-7371-4afb-bbb2-27a1a4373224\" class=\"img img-fluid o_we_custom_image\" style=\"width: 50%;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:none;\" border=\"1\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003cthead\u003e\u003ctr\u003e\n\u003cth style=\"border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e100 TESTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e500 TESTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e2500 TESTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e10000 TESTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eTag1-IL2 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e40×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e250μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1000μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eTag2-IL2R protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e4μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e20μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e100μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eBasiliximab\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e3μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e200μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eAnti-Tag1 Eu dye antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e250μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1000μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eAnti-Tag2 Ac antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e12.5×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e40μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e200μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1000μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e4000μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e40mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\u003c\/figure\u003e\u003cp style=\"margin:12.0pt 0cm;text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:white;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e*Repeated freeze-thaw cycles should be avoided. Aliquot upon first thaw and store according to the specified conditions. Valid for one year.\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"500T","offer_id":42332757327947,"sku":"UA086010-500T","price":2180.0,"currency_code":"USD","in_stock":true},{"title":"2500T","offer_id":42332757360715,"sku":"UA086010-2500T","price":8175.0,"currency_code":"USD","in_stock":true},{"title":"10000T","offer_id":42332757393483,"sku":"UA086010-10000T","price":21800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/006f0129-38bb-4cb7-8c83-46a74d74e72d.png?v=1788123766"},{"product_id":"unione-tr-fret-human-il3il3r-binding-kit-ua086011","title":"UniOne® TR-FRET Human IL3\/IL3R Binding Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-80℃\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-size:11.0pt;color:#0F1115\"\u003eThis kit utilizes Homogeneous Time-Resolved Fluorescence(TR-FRET) technology to measure the interaction between IL3 and IL3R. Thismethod enables simple and rapid high-throughput screening of inhibitors andblocking antibodies.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:11.0pt;color:#0F1115\"\u003eAs shown, the interaction between IL3 and IL3R isdetected using a Eu-labeled Anti-Tag1 Antibody (TR-FRET donor) and anAcceptor-labeled IL3 protein (TR-FRET acceptor). The binding of IL3 to IL3Rbrings the donor and acceptor antibodies into proximity. Excitation of thedonor antibody triggers Fluorescence Resonance Energy Transfer (FRET) to theacceptor antibody, resulting in a specific emission signal at 665nm. Thisspecific signal is proportional to the degree of interaction between IL3 andIL3R. This homogeneous assay is simple to perform and requires no washingsteps.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/product.template\/20289120\/6411c6e9-9655-4c5a-bb02-059d4c8f3994.png?access_token=921a4dc3-028f-495d-8357-3ec7279889c8\" class=\"img img-fluid o_we_custom_image\" style=\"width: 50%;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"background-color:white;margin:12.0pt 0cm;text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:#0F1115;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eTable 1. Kit Components and Storage Conditions\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse !msorm;border:none !msorm;\" border=\"1\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003cthead\u003e\u003ctr\u003e\n\u003cth style=\"border:solid windowtext 1.0pt !msorm;padding:7.5pt 12.0pt 7.5pt 0cm !msorm;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none !msorm;border:solid windowtext 1.0pt !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none !msorm;border:solid windowtext 1.0pt !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e100 TESTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none !msorm;border:solid windowtext 1.0pt !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e500 TESTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none !msorm;border:solid windowtext 1.0pt !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e2500 TESTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none !msorm;border:solid windowtext 1.0pt !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e10000 TESTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none !msorm;border:solid windowtext 1.0pt !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none !msorm;border:solid windowtext 1.0pt !msorm;padding:7.5pt 12.0pt 7.5pt 0cm !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eIL3-01A protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e250μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1000μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 0cm 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none !msorm;border:solid windowtext 1.0pt !msorm;padding:7.5pt 12.0pt 7.5pt 0cm !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eTag1-IL3R protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e40μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e200μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e800μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 0cm 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none !msorm;border:solid windowtext 1.0pt !msorm;padding:7.5pt 12.0pt 7.5pt 0cm !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eAnti-Tag1 Eu dye antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e250μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1000μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 0cm 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none !msorm;border:solid windowtext 1.0pt !msorm;padding:7.5pt 12.0pt 7.5pt 0cm !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e40mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 0cm 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\u003c\/figure\u003e\u003cp style=\"background-color:white;margin:12.0pt 0cm;text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:#0F1115;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e*Repeated freeze-thaw cycles should be avoided. Aliquot upon first thaw and store according to the specified conditions. Valid for one year.\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"500T","offer_id":42332757426251,"sku":"UA086011-500T","price":2180.0,"currency_code":"USD","in_stock":true},{"title":"2500T","offer_id":42332757459019,"sku":"UA086011-2500T","price":8175.0,"currency_code":"USD","in_stock":true},{"title":"10000T","offer_id":42332757491787,"sku":"UA086011-10000T","price":21800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/2fe4d8233caa40e88a50c03bb3e73ad8.png?v=1788123768"},{"product_id":"unione-tr-fret-human-il4il4ra-binding-kit-ua086012","title":"UniOne® TR-FRET Human IL4\/IL4RA Binding Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-80℃\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-size:11.0pt;color:#0F1115\"\u003eThis kit employs Homogeneous Time-Resolved Fluorescence(TR-FRET) technology to measure the interaction between IL4 and IL4R. Thismethod enables simple and rapid high-throughput screening of inhibitors andantibody blockers.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:11.0pt;color:#0F1115\"\u003eAs illustrated, the interaction between IL4 and IL4R isdetected using an Eu-labeled Anti-Tag1 antibody (TR-FRET donor) and anAcceptor-labeled IL4 protein (TR-FRET acceptor). The binding of IL4 to IL4Rbrings the donor antibody and the acceptor-labeled IL4 into close proximity.Excitation of the donor antibody triggers Fluorescence Resonance EnergyTransfer (FRET) to the acceptor antibody, resulting in a specific emissionsignal at 665 nm. The intensity of this specific signal is proportional to theextent of the IL4 and IL4R interaction. This homogeneous assay is simple toperform and requires no washing steps.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/product.template\/20289127\/2b66edf2-28be-408e-8be4-e8e4f3da762c.png?access_token=79d64206-7eb9-406a-a400-2d1b3dbebd63\" class=\"img img-fluid o_we_custom_image\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"500T","offer_id":42332757524555,"sku":"UA086012-500T","price":2180.0,"currency_code":"USD","in_stock":true},{"title":"2500T","offer_id":42332757557323,"sku":"UA086012-2500T","price":8175.0,"currency_code":"USD","in_stock":true},{"title":"10000T","offer_id":42332757590091,"sku":"UA086012-10000T","price":21800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_aa136b79-84b5-4ce0-ac5e-94bd4ec0eb4b.png?v=1788519779"},{"product_id":"unione-tr-fret-human-il6il6r-binding-kit-ua086013","title":"UniOne® TR-FRET Human IL6\/IL6R Binding Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-80℃\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003eThe assay kit employs Homogeneous Time-Resolved Fluorescence (TR-FRET) technology to measure the interaction between IL-6 and IL-6R. This method enables simple, rapid, and high-throughput identification of inhibitors and antibody-based blockers. As illustrated, the interaction between IL-6 and IL-6R is detected using a Eu-labeled anti-Tag1 antibody (TR-FRET donor) and an Acceptor-labeled anti-Tag2 antibody (TR-FRET acceptor). Upon binding of IL-6 to IL-6R, the donor and acceptor antibodies are brought into close proximity. Excitation of the donor antibody then triggers Fluorescence Resonance Energy Transfer (FRET) to the acceptor antibody, resulting in specific emission of a 665 nm wavelength signal from the acceptor. The intensity of this specific signal is proportional to the extent of IL-6\/IL-6R interaction. This homogeneous assay features a straightforward workflow with no washing steps required.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cimg class=\"img img-fluid o_we_custom_image\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/product.template\/20289155\/f37bd3c3-2703-471c-af33-1397de31484d.png?access_token=52356388-1326-4177-80f8-4fd79adabe7e\" alt=\"\" width=\"641\" height=\"285\"\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"background-color:white;margin:12.0pt 0cm;text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:#0F1115;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eTable 1. Reagents and Storage Conditions\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse !msorm;border:none !msorm;\" border=\"1\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003cthead\u003e\u003ctr\u003e\n\u003cth style=\"border:solid windowtext 1.0pt !msorm;padding:7.5pt 12.0pt 7.5pt 0cm !msorm;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none !msorm;border:solid windowtext 1.0pt !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none !msorm;border:solid windowtext 1.0pt !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e500 TESTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none !msorm;border:solid windowtext 1.0pt !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e2500 TESTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none !msorm;border:solid windowtext 1.0pt !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e10000 TESTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none !msorm;border:solid windowtext 1.0pt !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none !msorm;border:solid windowtext 1.0pt !msorm;padding:7.5pt 12.0pt 7.5pt 0cm !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eTag1-IL6 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e20μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e100μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 0cm 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none !msorm;border:solid windowtext 1.0pt !msorm;padding:7.5pt 12.0pt 7.5pt 0cm !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eTag2-IL-6R protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e20μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e100μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 0cm 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none !msorm;border:solid windowtext 1.0pt !msorm;padding:7.5pt 12.0pt 7.5pt 0cm !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eTocilizumab\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10 μM\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e200 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 0cm 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none !msorm;border:solid windowtext 1.0pt !msorm;padding:7.5pt 12.0pt 7.5pt 0cm !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eAnti-Tag1 Eu cryptate antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e250μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1000μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 0cm 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none !msorm;border:solid windowtext 1.0pt !msorm;padding:7.5pt 12.0pt 7.5pt 0cm !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eAnti-Tag2 Acceptor antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e12.5×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e200μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1000μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e4000μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 0cm 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none !msorm;border:solid windowtext 1.0pt !msorm;padding:7.5pt 12.0pt 7.5pt 0cm !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e40mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 0cm 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cspan style=\"color:#0F1115;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e* Avoid repeated freeze-thaw cycles of reagents. Aliquot upon first th\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"100T","offer_id":42332757622859,"sku":"UA086013-100T","price":730.0,"currency_code":"USD","in_stock":true},{"title":"500T","offer_id":42332757655627,"sku":"UA086013-500T","price":2180.0,"currency_code":"USD","in_stock":true},{"title":"2500T","offer_id":42332757688395,"sku":"UA086013-2500T","price":8175.0,"currency_code":"USD","in_stock":true},{"title":"10000T","offer_id":42332757721163,"sku":"UA086013-10000T","price":21800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_b0ba9107-59a9-4818-892e-3f99712372f4.png?v=1788519781"},{"product_id":"unione-tr-fret-human-il17ail17ra-binding-kit-ua086014","title":"Unione® TR-FRET Human IL17A\/IL17RA Binding Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe kit employs homogeneous time-resolved fluorescence technology.\u003c\/span\u003e\u003cspan\u003eTR-FRET\u003c\/span\u003e\u003cspan\u003e), used for the determination of\u003c\/span\u003e\u003cspan\u003eIL17A\u003c\/span\u003e\u003cspan\u003ewith\u003c\/span\u003e\u003cspan\u003eIL17RA\u003c\/span\u003e\u003cspan\u003ethe interactions between them. This method enables simple and rapid high-throughput identification of inhibitors and antibody blockers.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/36a8a3aa-491e-4f32-9790-1cac4a63505e.png\" style=\"margin-left:30px; width:324px; height:auto; vertical-align:middle;\"\u003e\u003c\/p\u003e\n\u003cspan\u003eAs shown in the figure,\u003c\/span\u003e\u003cspan\u003eIL17A\u003c\/span\u003e\u003cspan\u003ewith\u003c\/span\u003e\u003cspan\u003eIL17 RA\u003c\/span\u003e\u003cspan\u003eThe interaction between them is achieved by using\u003c\/span\u003e\u003cspan\u003eI\u003c\/span\u003e\u003cspan\u003eMarked anti-\u003c\/span\u003e\u003cspan\u003eTag1\u003c\/span\u003e\u003cspan\u003eAntibody (\u003c\/span\u003e\u003cspan\u003eTR-FRET\u003c\/span\u003e\u003cspan\u003eDonor) and\u003c\/span\u003e\u003cspan\u003eAc\u003c\/span\u003e\u003cspan\u003eMarked\u003c\/span\u003e\u003cspan\u003eTag2\u003c\/span\u003e\u003cspan\u003eAntibody (\u003c\/span\u003e\u003cspan\u003eTR-FRET\u003c\/span\u003e\u003cspan\u003e(receptor) testing. Due to\u003c\/span\u003e\u003cspan\u003eIL17A\u003c\/span\u003e\u003cspan\u003ewith\u003c\/span\u003e\u003cspan\u003eIL17 RA\u003c\/span\u003e\u003cspan\u003eBy bringing the donor antibody and the acceptor antibody into close proximity, excitation of the donor antibody triggers fluorescence resonance energy transfer to the acceptor antibody.\u003c\/span\u003e\u003cspan\u003eFörster resonance energy transfer\u003c\/span\u003e\u003cspan\u003e), enabling the receptor antibody to emit specifically.\u003c\/span\u003e\u003cspan\u003e665 nm\u003c\/span\u003e\u003cspan\u003eA signal of a specific wavelength.\u003c\/span\u003e\u003cspan\u003eSecukinumab\u003c\/span\u003e\u003cspan\u003ePositive drugs can block.\u003c\/span\u003e\u003cspan\u003eIL17A\u003c\/span\u003e\u003cspan\u003ewith\u003c\/span\u003e\u003cspan\u003eIL17 RA\u003c\/span\u003e\u003cspan\u003eThe combination of, makes\u003c\/span\u003e\u003cspan\u003eFRET\u003c\/span\u003e\u003cspan\u003eThe signal cannot be generated, and the screened drug affects…\u003c\/span\u003e\u003cspan\u003eIL17A\u003c\/span\u003e\u003cspan\u003ewith\u003c\/span\u003e\u003cspan\u003eIL17 RA\u003c\/span\u003e\u003cspan\u003eThe stronger the blocking effect, the lower the signal. This specific signal is directly proportional to the extent of interaction between\u003c\/span\u003e\u003cspan\u003eIL17A\u003c\/span\u003e\u003cspan\u003eand\u003c\/span\u003e\u003cspan\u003eIL17 RA.\u003c\/span\u003e\u003cspan\u003eThis homogeneous assay is easy to perform and requires no washing steps.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003ctable border=\"1\" style=\"border-collapse:collapse;width:auto;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eComponent\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eConcentration\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e100T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e2500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e10000T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eStorage Temperature\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag1- IL17A protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e400\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag2- IL17 RA protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e400\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAnti-Tag1 Eu antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003e0\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e250\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100\u003c\/span\u003e\u003cspan\u003e0μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAnti-Tag2 Ac antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e12.5\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e40\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e0\u003c\/span\u003e\u003cspan\u003e0μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100\u003c\/span\u003e\u003cspan\u003e0μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e40\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e400\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003emL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003emL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e40\u003c\/span\u003e\u003cspan\u003emL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan\u003eCaution: After the first thaw, immediately aliquot and store at the recommended temperature. Avoid storing diluted samples or subjecting them to repeated freeze-thaw cycles.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e,\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eReagent preparation\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e.1\u003c\/span\u003e\u003cspan\u003eBefore use, thaw all reagents at room temperature (allow to equilibrate at room temperature for at least 30 minutes). The reaction volume in a 384-well plate is 20 µL.\u003c\/span\u003e\u003cspan\u003emu\u003c\/span\u003e\u003cspan\u003eL (the reagent volumes for the reaction system are shown in the table). Calculate the required volume before preparation and prepare accordingly. The following formulation is for reference only, using 500 reactions as an example.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eTable 1\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003cspan\u003eReagent preparation\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eReagent name\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eConfiguration\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eμL per test well\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDetection\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003ebuffer\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eTake 2\u003c\/span\u003e\u003cspan\u003e10 mL\u003c\/span\u003e\u003cspan\u003etimes\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eDetection\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eAdd 18 mL of deionized water to the buffer, dilute.\u003c\/span\u003e\u003cspan\u003eExplanation\u003c\/span\u003e\u003cspan\u003eto\u003c\/span\u003e\u003cspan\u003e1×, mixed\u003c\/span\u003e\u003cspan\u003eEvenly prepared\u003c\/span\u003e\u003cspan\u003euse\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eSample to be tested\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eAccording to the reaction system, the sample to be tested is diluted with 1× Detection buffer to the desired concentration.\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003cspan\u003eIf DMSO is used, ensure that the DMSO concentration is consistent across all assay wells.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag1- IL17A protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003etake\u003c\/span\u003e\u003cspan\u003e20 μL Tag1-IL17A protein\u003c\/span\u003e\u003cspan\u003eUndiluted solution,\u003c\/span\u003e\u003cspan\u003e1× Detection Buffer\u003c\/span\u003e\u003cspan\u003eDilute to\u003c\/span\u003e\u003cspan\u003e2 mL,\u003c\/span\u003e\u003cspan\u003eMix well and set aside.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag2- IL17 RA protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003etake\u003c\/span\u003e\u003cspan\u003e20 μL Tag2-IL17 RA protein\u003c\/span\u003e\u003cspan\u003eUndiluted solution,\u003c\/span\u003e\u003cspan\u003e1× Detection Buffer\u003c\/span\u003e\u003cspan\u003eDilute to\u003c\/span\u003e\u003cspan\u003e2 mL,\u003c\/span\u003e\u003cspan\u003eMix well and set aside.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAntibody Mix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003etake\u003c\/span\u003e\u003cspan\u003e50 μL Anti-Tag1 Eu antibody\u003c\/span\u003e\u003cspan\u003eConcentrate, add\u003c\/span\u003e\u003cspan\u003e1× Detection buffer 2.45 mL,\u003c\/span\u003e\u003cspan\u003eMix well and set aside.\u003c\/span\u003e\u003cspan\u003e;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003etake\u003c\/span\u003e\u003cspan\u003e200 μL Anti-Tag2 Ac antibody\u003c\/span\u003e\u003cspan\u003eConcentrate, add\u003c\/span\u003e\u003cspan\u003e1× Detection buffer 2.3 mL,\u003c\/span\u003e\u003cspan\u003eMix well and set aside.\u003c\/span\u003e\u003cspan\u003e;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eAnti-Tag1 Eu antibody\u003c\/span\u003e\u003cspan\u003eand\u003c\/span\u003e\u003cspan\u003eAnti-Tag2 Ac antibody\u003c\/span\u003e\u003cspan\u003eAfter separate dilutions\u003c\/span\u003e\u003cspan\u003e1:1\u003c\/span\u003e\u003cspan\u003eMix well and set aside.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan\u003e1.2\u003c\/span\u003e\u003cspan\u003eSample to be tested\u003c\/span\u003e\u003cspan\u003eGradient dilution\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003ewith\u003c\/span\u003e\u003cspan\u003eSecukinumab\u003c\/span\u003e\u003cspan\u003eFor example, the diluent is 1× Detection buffer.\u003c\/span\u003e\u003cspan\u003eTo minimize the impact of matrix effects, it is recommended to dilute the sample with a solution that has the same matrix as the analyte; and\u003c\/span\u003e\u003cspan\u003eThe sample to be tested should be adjusted according to its actual concentration.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eTable\u003c\/span\u003e\u003cspan\u003e2.\u003c\/span\u003e\u003cspan\u003ePositive drug S\u003c\/span\u003e\u003cspan\u003esecukinumab\u003c\/span\u003e\u003cspan\u003eGradient dilution\u003c\/span\u003e\u003cspan\u003e(Adjust according to actual conditions)\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eSecukinumab\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eFinal concentration\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003enM\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eSecukinumab\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003ePreparation concentration\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003enM\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003ePreparation method\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e①\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003cspan\u003e100\u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan\u003eM\u003c\/span\u003e\u003cspan\u003eS\u003c\/span\u003e\u003cspan\u003esecukinumab\u003c\/span\u003e\u003cspan\u003e+99\u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e②\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e33.33\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e333.33\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20\u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e①\u003c\/span\u003e\u003cspan\u003e+40\u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e③\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e11.11\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e111.11\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20\u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e②\u003c\/span\u003e\u003cspan\u003e+40\u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e④\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3.70\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e37.04\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20\u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e③\u003c\/span\u003e\u003cspan\u003e+40\u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑤\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1.23\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e12.35\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20\u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e④\u003c\/span\u003e\u003cspan\u003e+40\u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑥\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e0.41\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4.12\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20\u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e⑤\u003c\/span\u003e\u003cspan\u003e+40\u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑦\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e0.14\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1.37\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20\u003c\/span\u003e\u003cspan\u003emu\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e⑥\u003c\/span\u003e\u003cspan\u003e+40\u003c\/span\u003e\u003cspan\u003emu\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑧\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e0.05\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e0.46\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20\u003c\/span\u003e\u003cspan\u003emu\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e⑦\u003c\/span\u003e\u003cspan\u003e+40\u003c\/span\u003e\u003cspan\u003emu\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑨\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e0.015\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e0.15\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20\u003c\/span\u003e\u003cspan\u003emu\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e⑧\u003c\/span\u003e\u003cspan\u003e+40\u003c\/span\u003e\u003cspan\u003emu\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eBlank\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e40\u003c\/span\u003e\u003cspan\u003emu\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003e2\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e,\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eSample addition and controls\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.1\u003c\/span\u003e\u003cspan\u003eSample addition order for the experimental wells:\u003c\/span\u003e\u003cspan\u003e2 μL\u003c\/span\u003e\u003cspan\u003eSample to be tested,\u003c\/span\u003e\u003cspan\u003e4 μL Tag1-IL17A protein\u003c\/span\u003e\u003cspan\u003eWorking fluid,\u003c\/span\u003e\u003cspan\u003e4 μL Tag2-IL17 RA protein\u003c\/span\u003e\u003cspan\u003eWorking fluid,\u003c\/span\u003e\u003cspan\u003e10 μL Antibody Mix\u003c\/span\u003e\u003cspan\u003e, add in sequence\u003c\/span\u003e\u003cspan\u003e384\u003c\/span\u003e\u003cspan\u003eIn the shallow-hole plate;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.2\u003c\/span\u003e\u003cspan\u003eMaximum value reference hole:\u003c\/span\u003e\u003cspan\u003e2 μL 1× Detection buffer\u003c\/span\u003e\u003cspan\u003e,\u003c\/span\u003e\u003cspan\u003e4 μL Tag1-IL17A protein\u003c\/span\u003e\u003cspan\u003eWorking fluid,\u003c\/span\u003e\u003cspan\u003e4 μL Tag2-IL17 RA protein\u003c\/span\u003e\u003cspan\u003eWorking fluid\u003c\/span\u003e\u003cspan\u003e,\u003c\/span\u003e\u003cspan\u003e10 μL Antibody Mix\u003c\/span\u003e\u003cspan\u003e, add in sequence\u003c\/span\u003e\u003cspan\u003e384\u003c\/span\u003e\u003cspan\u003eIn the shallow-hole plate\u003c\/span\u003e\u003cspan\u003e;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.3 Quality control well(\u003c\/span\u003e\u003cspan\u003eNC\u003c\/span\u003e\u003cspan\u003e):\u003c\/span\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003eAdd μL of 1× Detection buffer.\u003c\/span\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eAntibody\u003c\/span\u003e\u003cspan\u003eMix\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAfter all samples have been added, centrifuge the plate, seal it with a plate seal, and incubate at room temperature for 2 hours.\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse:collapse;width:auto;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eMaximum value\u003c\/span\u003e\u003cspan\u003eComparison\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003esample\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eNC\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eStep one\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2 μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1× Detection\u003c\/span\u003e\u003cspan\u003eB\u003c\/span\u003e\u003cspan\u003euffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003emu\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eGradient dilution of the sample to be tested\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"4\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e0\u003c\/span\u003e\u003cspan\u003euL 1× Detection\u003c\/span\u003e\u003cspan\u003eB\u003c\/span\u003e\u003cspan\u003euffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"2\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4 μL Tag1-IL17A protein\u003c\/span\u003e\u003cspan\u003eWorking fluid\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"2\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate for 10 minutes.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"2\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eStep two\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"2\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4 μL\u003c\/span\u003e\u003cspan\u003eTag2- IL17 RA protein\u003c\/span\u003e\u003cspan\u003en\u003c\/span\u003e\u003cspan\u003eWorking fluid\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003cspan\u003eMix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eStep three\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCover with a sealing film and incubate at room temperature for 2 hours.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e3.\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eDetection\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eDetect on a TR-FRET-compatible microplate reader.\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003cspan\u003eThe excitation light is 320\/340 nm, and detection is performed at 620 nm and 665 nm.\u003c\/span\u003e\u003cspan\u003epost\u003c\/span\u003e\u003cspan\u003eWavelength of the radiation.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e[Result Calculation]\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1)\u003c\/span\u003e\u003cspan\u003eCalculate signal value\u003c\/span\u003e\u003cspan\u003e(Ratio)\u003c\/span\u003e\u003cspan\u003eThe 665 nm fluorescence signal is multiplied by 10,000 compared to the 620 nm fluorescence signal.\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eRatio\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e=\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e(665\/620)\u003c\/span\u003e\u003cspan\u003etimes\u003c\/span\u003e\u003cspan\u003e10000\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2)\u003c\/span\u003e\u003cspan\u003eCalculate based on the signal value.\u003c\/span\u003e\u003cspan\u003eNet signal\u003c\/span\u003e\u003cspan\u003e:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eNet signal\u003c\/span\u003e\u003cspan\u003e= (Std-\u003c\/span\u003e\u003cspan\u003eNC\u003c\/span\u003e\u003cspan\u003e)\/\u003c\/span\u003e\u003cspan\u003eNC\u003c\/span\u003e\u003cspan\u003e×100\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3)\u003c\/span\u003e\u003cspan\u003eCalculate CV (%)\u003c\/span\u003e\u003cspan\u003e:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eCV (%) = Standard Deviation\/Mean Ratio × 100%\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e[Data Example]\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe following data should not be used as a substitute for the data obtained in the experiment; it is provided for illustrative purposes only, and the results may\u003c\/span\u003e\u003cspan\u003eDue to the reading board\u003c\/span\u003e\u003cspan\u003eInstrument\u003c\/span\u003e\u003cspan\u003eIt varies from device to device.\u003c\/span\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/de72a334-14cf-469a-be84-cd799d92d8f8.png\" style=\"margin-left:30px; width:494px; height:auto; vertical-align:middle;\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003eNote: Recommended\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eMicroplate (384-well plate, white, flat-bottom)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"500T","offer_id":42332757786699,"sku":"UA086014-500T","price":2180.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":42332757753931,"sku":"UA086014-100T","price":730.0,"currency_code":"USD","in_stock":true},{"title":"2500T","offer_id":42332757819467,"sku":"UA086014-2500T","price":8175.0,"currency_code":"USD","in_stock":true},{"title":"10000T","offer_id":42332757852235,"sku":"UA086014-10000T","price":21800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_e5500213-5fbb-46a5-a68f-d6bb77fd0986.png?v=1790280035"},{"product_id":"unione-tr-fret-human-il21il21r-binding-kit-ua086015","title":"UniOne® TR-FRET Human IL21\/IL21R Binding Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-80℃\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-size:11.0pt;color:#0F1115\"\u003eThis kit utilizes Homogeneous Time-Resolved Fluorescence(TR-FRET) technology to measure the interaction between IL21 and IL21R. Thismethod enables simple and rapid high-throughput screening of inhibitors andantibody blockers.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:11.0pt;color:#0F1115\"\u003eAs illustrated, the interaction between IL21 and IL21R isdetected using an Eu-labeled anti-Tag1 antibody (TR-FRET donor) and anAcceptor-labeled anti-Tag2 antibody (TR-FRET acceptor). The binding of IL21 toIL21R brings the donor and acceptor antibodies into proximity. Excitation ofthe donor antibody then triggers Fluorescence Resonance Energy Transfer (FRET)to the acceptor antibody, resulting in a specific emission signal at 665 nm.The intensity of this specific signal is proportional to the extent of theIL21\/IL21R interaction. This homogeneous assay is simple to perform andrequires no washing steps.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/product.template\/20289179\/ad38ddef-93c6-442c-bda0-bed4c75750c3.png?access_token=173fdb60-349a-40e7-9c54-7ca3c75912cd\" class=\"img img-fluid o_we_custom_image\" style=\"width: 50%;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"background-color:white;margin:12.0pt 0cm;text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:#0F1115;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eTable 1. Kit Components and Storage Temperature\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse !msorm;border:none !msorm;\" border=\"1\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003cthead\u003e\u003ctr\u003e\n\u003cth style=\"border:solid windowtext 1.0pt !msorm;padding:7.5pt 12.0pt 7.5pt 0cm !msorm;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none !msorm;border:solid windowtext 1.0pt !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none !msorm;border:solid windowtext 1.0pt !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e500 TESTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none !msorm;border:solid windowtext 1.0pt !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e2500 TESTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none !msorm;border:solid windowtext 1.0pt !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e10000 TESTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none !msorm;border:solid windowtext 1.0pt !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none !msorm;border:solid windowtext 1.0pt !msorm;padding:7.5pt 12.0pt 7.5pt 0cm !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eTag1-IL21 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e20μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e100μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 0cm 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none !msorm;border:solid windowtext 1.0pt !msorm;padding:7.5pt 12.0pt 7.5pt 0cm !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eTag2-IL21R protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e20μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e100μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 0cm 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none !msorm;border:solid windowtext 1.0pt !msorm;padding:7.5pt 12.0pt 7.5pt 0cm !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eAnti-Tag1 Eu dye antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e250μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1000μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 0cm 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none !msorm;border:solid windowtext 1.0pt !msorm;padding:7.5pt 12.0pt 7.5pt 0cm !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eAnti-Tag2 Ac antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e12.5×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e200μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1000μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e4000μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 0cm 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none !msorm;border:solid windowtext 1.0pt !msorm;padding:7.5pt 12.0pt 7.5pt 0cm !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 12.0pt 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e40mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt !msorm;border-left:none !msorm;border-right:solid windowtext 1.0pt !msorm;border-top:none !msorm;padding:7.5pt 0cm 7.5pt 12.0pt !msorm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\u003c\/figure\u003e\u003cp style=\"background-color:white;margin:12.0pt 0cm;text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:#0F1115;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e*Reagents should be protected from repeated freeze-thaw cycles. It is recommended to aliquot upon first thaw and store under the specified conditions. The shelf life is one year.\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"500T","offer_id":42332757885003,"sku":"UA086015-500T","price":2180.0,"currency_code":"USD","in_stock":true},{"title":"2500T","offer_id":42332757917771,"sku":"UA086015-2500T","price":8175.0,"currency_code":"USD","in_stock":true},{"title":"10000T","offer_id":42332757950539,"sku":"UA086015-10000T","price":21800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/117190f2c3444222bdee4b4f44b533cb.png?v=1788123804"},{"product_id":"unione-tr-fret-human-il10il10ra-binding-kit-ua086016","title":"UniOne® TR-FRET Human IL10\/IL10RA Binding Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-80℃\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-size:11.0pt\"\u003eThis kit utilizes HomogeneousTime-Resolved Fluorescence (TR-FRET) technology to measure the interactionbetween IL10A and IL10RA. This method enables simple and rapid high-throughputscreening of inhibitors and antibody blockers.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:11.0pt\"\u003eAs illustrated, the interactionbetween IL10A and IL10RA is detected using an Eu-labeled Anti-Tag1 antibody(TR-FRET donor) and an Acceptor-labeled IL10A protein (TR-FRET acceptor). Thebinding of IL10A to IL10RA brings the donor antibody and the acceptor proteininto close proximity. Excitation of the donor antibody then triggersFluorescence Resonance Energy Transfer (FRET) to the acceptor antibody,resulting in the emission of a specific signal at 665nm. The intensity of thisspecific signal is proportional to the extent of the IL10A and IL10RAinteraction. This homogeneous assay is simple to perform and requires nowashing steps.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/product.template\/20289188\/5d36d3ee-7a0f-4843-9ba6-d5dcda751b45.png?access_token=b0ee5034-0573-484c-aff5-defd9b923e29\" class=\"img img-fluid o_we_custom_image\" style=\"width: 50%;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"margin:12.0pt 0cm;text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003eKit Components\u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003e】\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:none;\" border=\"1\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003cthead\u003e\u003ctr\u003e\n\u003cth style=\"border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e500 TESTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e2500 TESTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e10000 TESTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eIL10A-01A protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e25μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e125μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e500μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eTag1- IL10 RA protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e40μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e200μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e800μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eAnti-Tag1 Eu dye antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e250μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1000μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e40mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cspan style=\"color:white;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e*Reagents should be protected from repeated freeze-thaw cycles. It is recommended to aliquot upon first thaw and store according to the \u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003especified conditions. The shelf life is one year.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"500T","offer_id":42332757983307,"sku":"UA086016-500T","price":2180.0,"currency_code":"USD","in_stock":true},{"title":"2500T","offer_id":42332758016075,"sku":"UA086016-2500T","price":8175.0,"currency_code":"USD","in_stock":true},{"title":"10000T","offer_id":42332758048843,"sku":"UA086016-10000T","price":21800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/97e91f2349b64ea091fa355991825d10.png?v=1788123807"},{"product_id":"unione-tr-fret-human-bcl-xlcrbn-protac-binding-kit-ua086017","title":"UniOne® TR-FRET Human BCL-XL\/CRBN PROTAC Binding Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-80℃\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eThe assay kit employs homogeneous time-resolved fluorescence (TR-FRET) technology to measure the interaction between the DDB1\/CRBN complex and BCL-XL mediated by the molecular glue XZ739 and test compounds. This method enables a simple, rapid, and high-throughput screening of small molecules capable of mediating the interaction between the DDB1\/CRBN complex and BCL-XL.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eAs shown in the figure below, the interaction between DDB1\/CRBN and BCL-XL is detected using a Eu-labeled anti-Tag1 antibody (TR-FRET donor) and an acceptor-labeled anti-Tag2 antibody (TR-FRET acceptor). The molecular glue XZ739 mediates the interaction between DDB1\/CRBN and BCL-XL, bringing the donor and acceptor antibodies into proximity. Excitation of the donor antibody triggers fluorescence resonance energy transfer (FRET) to the acceptor antibody, resulting in a specific emission signal at 665 nm. This signal is proportional to the extent of interaction mediated by XZ739 with DDB1\/CRBN and BCL-XL. The homogeneous assay is simple to perform and requires no washing steps.\u003c\/span\u003e\u003cspan\u003e      \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/a87a39c5-d556-4218-818b-a4632c8ae8d6.png\" style=\"margin-left:30px; width:544px; height:auto; vertical-align:middle;\" alt=\"\"\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003eHere is the translated content in English while preserving the original formatting:```html\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse:collapse;width:auto;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eComponent\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eConcentration\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e100T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e2500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e10000T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eStorage Temperature\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag1-CRBN protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e8\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e40μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e200μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e800μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag2- \u003c\/span\u003e\u003cspan\u003eBCL-XL \u003c\/span\u003e\u003cspan\u003eprotein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eXZ739\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4m\u003c\/span\u003e\u003cspan\u003eM\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e25\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAnti-Tag1 Eu antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003e0×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e25\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e125\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAnti-Tag2 Ac antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e25\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e000μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e40mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan\u003eNote: Aliquot all components immediately after first thawing and store at the recommended temperature. Avoid storage after dilution and repeated freeze-thaw cycles.\u003c\/span\u003e\u003c\/p\u003e```\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003e1. Reagent Preparation\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003e1.1 Before use, thaw all reagents at room temperature (equilibrate at room temperature for at least 30 min). The reaction volume for a 384-well shallow well plate is 20 μL (reagent volumes for the reaction system are shown in the table). Calculate the required volume before preparation and prepare as needed; the following preparation is for reference only, using 500 tests as an example.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003eTable 1. Reagent Preparation\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\" style=\"width:100%;\"\u003e\u003ctable class=\"ck-table-resized\" style=\"border-collapse:collapse;border:1px solid #000000;width:auto;\"\u003e\n\u003ccolgroup\u003e\n\u003ccol style=\"width:9.98%;\"\u003e\n\u003ccol style=\"width:81.14%;\"\u003e\n\u003ccol style=\"width:8.88%;\"\u003e\n\u003c\/colgroup\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003eReagent Name\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003ePreparation\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003eVolume per Detection Well (μL)\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eDetection buffer \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:justify;\"\u003e\u003cspan style=\"color:#000000;\"\u003eAdd 2 mL of 10× Detection buffer 1 to 18 mL of deionized water, dilute to 1×, mix well, and set aside.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e-\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eXZ739\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:justify;\"\u003e\u003cspan style=\"color:#000000;\"\u003eAccording to the reaction system, dilute the compound to the desired concentration using 1× Detection buffer.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eTag1-CRBN protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:justify;\"\u003e\u003cspan style=\"color:#000000;\"\u003eTake 40 μL of Tag1-CRBN protein stock solution, dilute to 2 mL with 1× Detection buffer, \u003cstrong\u003emix well, and set aside.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eTag2- BCL-XL protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:justify;\"\u003e\u003cspan style=\"color:#000000;\"\u003eTake 20 μL of Tag2-BCL-XL protein stock solution, dilute to 2 mL with 1× Detection buffer, \u003cstrong\u003emix well, and set aside.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eAntibody Mix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:justify;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003eTake 25 μL of Anti-Tag1 Eu antibody \u003c\/strong\u003estock solution, add 2.475 mL of 1× Detection buffer, \u003cstrong\u003emix well; take 100 μL of Anti-Tag2 Ac antibody \u003c\/strong\u003estock solution, add 2.4 mL of 1× Detection buffer, \u003cstrong\u003emix well;\u003c\/strong\u003e mix both solutions 1:1 to prepare the Antibody Mix.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e10\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003e1.2 Gradient Dilution of Test Samples\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003eUsing XZ739 as an example, the diluent is 1× Detection buffer. To minimize matrix effect interference, it is recommended to dilute with a solution matching the test sample matrix; adjust the test samples according to actual concentrations.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003eTable 2. XZ739 Gradient Dilution (Adjust According to Actual Conditions)\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:1px solid #000000;width:auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eXZ739 \u003cstrong\u003eFinal Concentration (nM)\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eXZ739 \u003cstrong\u003ePreparation Concentration (nM)\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003ePreparation Method\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e①\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e10000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e100000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e1μL 4mM XZ739 +39μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e②\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e5000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e50000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e20μL ① +20μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e③\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e2500\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e25000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e20μL ② +20μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e④\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e1250\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e12500\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e20μL ③ +20μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑤\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e625\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e6250\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e20μL ④ +20μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑥\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e312.50\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e3125\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e20μL ⑤ +20μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑦\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e156.25\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e1562.50\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e20μL ⑥ +20μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑧\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e78.13\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e781.25\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e20μL ⑦+20μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑨\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e39.06\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e390.63\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e20μL ⑧+20μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑩\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e19.53\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e195.31\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e20μL ⑨+20μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#333333;\"\u003e⑪\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e9.77\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e97.66\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e20μL ⑩+20μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eBlank\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e20μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003e2. Sample Addition and Controls\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003e2.1 Test Samples: Add 4 μL Tag1-CRBN protein working solution, 4 μL Tag2-BCL-XL protein working solution, 2 μL gradient-diluted test samples, and 10 μL mixed Antibody Mix sequentially into the 384-well shallow well plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003e2.2 Positive Control Standard Curve: 4 μL Tag1-CRBN protein working solution, 4 μL Tag2-BCL-XL protein working solution, 2 μL gradient-diluted XZ739, and 10 μL Antibody Mix.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003e2.3 Blank Control Wells: Replace test samples with 2 μL 1× Detection buffer;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003e2.4 NC (Negative Control): Add 10 μL 1× Detection buffer and 10 μL Antibody Mix.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003eAfter adding all samples, centrifuge, seal with a plate sealing film, and incubate at room temperature for 2 hours.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"margin-right:8px;\"\u003e3. \u003c\/span\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003eDetection\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:1px solid #000000;width:auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003ePositive Control Standard Curve\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003eTest Samples\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003eBlank Control Wells\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003eNC\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e2 μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eGradient-diluted XZ739\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e2 μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eGradient-diluted test samples\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e2 μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" rowspan=\"4\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e10 μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eAdd 10 μL Antibody Mix\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"3\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e4 μL Tag1-CRBN protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"3\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e4 μL Tag2-BCL-XL protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"3\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e10 μL Antibody Mix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"4\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eSeal the plate wells with a sealing film \u003cstrong\u003e,\u003c\/strong\u003e and incubate at room temperature for 2 hours;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003eDetect using a microplate reader compatible with TR-FRET. Excitation wavelength is 320\/340 nm, and emission wavelengths detected are 620 nm and 665 nm.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#1655A5;\"\u003e\u003cstrong\u003e[Result Calculation]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003e1) Calculate signal value (Ratio): Divide the fluorescence signal at 665 nm by the signal at 620 nm, then multiply by 10000.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003eRatio = (665\/620) ×10000\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003e2) Calculate Net signal based on the signal value:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003eNet signal = (Std-NC)\/NC×100\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003e3) Calculate CV (%):\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003eCV（%）= Standard Deviation\/Mean Ratio × 100%\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#1655A5;\"\u003e\u003cstrong\u003e[Data Example]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003eThe following data cannot replace experimental results and is provided only as an example; results may vary depending on the plate reader used.   \u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"image_resized\" style=\"height:auto;margin-left:30px;vertical-align:middle;width:465px;\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/e74f52f1-a2fc-45dd-82c0-20a39d9b871d.png\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#FFFFFF;\"\u003e3 \/3\u003c\/span\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003eNote: Recommended microplates (384-well, white, shallow well);\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"100T","offer_id":42968279023691,"sku":"UA086017-100T","price":730.0,"currency_code":"USD","in_stock":true},{"title":"500T","offer_id":42332758081611,"sku":"UA086017-500T","price":2180.0,"currency_code":"USD","in_stock":true},{"title":"2500T","offer_id":42332758114379,"sku":"UA086017-2500T","price":8175.0,"currency_code":"USD","in_stock":true},{"title":"10000T","offer_id":42332758147147,"sku":"UA086017-10000T","price":21800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_62ceec70-168b-4efd-8cd5-7d062632604a.png?v=1788519784"},{"product_id":"unione-tr-fret-human-epigeneous-bromodomain-binding-kit-ua086018","title":"UniOne® TR-FRET Human BRD4（BD1+BD2）\/H4 Binding Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-80℃\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-size:11.0pt;color:#0F1115\"\u003eThis kit utilizes Homogeneous Time-Resolved Fluorescence(TR-FRET) technology to measure the interaction between BRD4 and the[LYS(5,8,12,16)AC] H4(1-21) peptide. This method enables simple and rapidhigh-throughput screening of inhibitors and blocking antibodies.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:11.0pt;color:#0F1115\"\u003eAs illustrated, the interaction between BRD4 and the[LYS(5,8,12,16)AC] H4(1-21) peptide is detected using a Eu-labeled Anti-Tag1antibody (TR-FRET donor) and an Acceptor-labeled Anti-Tag2 antibody (TR-FRETacceptor). The binding of BRD4 to the [LYS(5,8,12,16)AC] H4(1-21) peptidebrings the donor and acceptor antibodies into close proximity. Excitation ofthe donor antibody then triggers Fluorescence Resonance Energy Transfer (FRET)to the acceptor antibody, resulting in a specific emission signal at 665 nm.The intensity of this specific signal is proportional to the degree ofinteraction between BRD4 and the [LYS(5,8,12,16)AC] H4(1-21) peptide. Thishomogeneous assay is simple to perform and requires no washing steps.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/product.template\/20289217\/d21586d2-da2e-423d-9d31-f6102103f404.png?access_token=275c5a7e-33c6-46f5-855b-74a1207e1d39\" class=\"img img-fluid o_we_custom_image\" style=\"width: 50%;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"background-color:white;margin:12.0pt 0cm;text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:#0F1115;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eTable 1. Kit Components and Storage Conditions\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:none;\" border=\"1\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003cthead\u003e\u003ctr\u003e\n\u003cth style=\"border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e100 TESTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e500 TESTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e2500 TESTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e10000 TESTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e(+)-JQ-1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e300μM\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e3μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e200μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eTag1-BRD4 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e4μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e20μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e100μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eTag2- peptide\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e4μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e20μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e100μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eAnti-Tag1 Eu dye antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e250μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1000μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eAnti-Tag2 Ac antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e12.5×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e40μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e200μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1000μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e4000μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e40mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\u003c\/figure\u003e\u003cp style=\"background-color:white;margin:12.0pt 0cm;text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:#0F1115;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e* Avoid repeated freeze-thaw cycles of reagents. Aliquot upon first thaw and store according to the specified conditions. Shelf life is one year.\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"500T","offer_id":42332758179915,"sku":"UA086018-500T","price":2180.0,"currency_code":"USD","in_stock":true},{"title":"2500T","offer_id":42332758212683,"sku":"UA086018-2500T","price":8175.0,"currency_code":"USD","in_stock":true},{"title":"10000T","offer_id":42332758245451,"sku":"UA086018-10000T","price":21800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/b1f107e0b02e46aca887bf23eb5a146a.png?v=1788123814"},{"product_id":"unione-tr-fret-human-bcl-xlvhl-protac-binding-kit-ua086019","title":"UniOne® TR-FRET Human BCL-XL\/VHL PROTAC Binding Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-80℃\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-size:11.0pt\"\u003eThis kit uses HomogeneousTime-Resolved Fluorescence (TR-FRET) technology to measure the molecular glueBCL-XL DEGRADER-2 and test compound-mediated interaction between VHL andBCL-XL. This method enables a simple and rapid high-throughput format for screeningsmall molecules that can mediate the interaction between VHL and BCL-XL.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:11.0pt\"\u003eAs shown, the interaction betweenVHL and BCL-XL is detected using an Eu-labeled anti-Tag1 antibody (TR-FRETdonor) and an Acceptor-labeled anti-Tag2 antibody (TR-FRET acceptor). Since themolecular glue BCL-XL DEGRADER-2 mediates the interaction between VHL andBCL-XL, the donor and acceptor antibodies are brought into proximity.Excitation of the donor antibody triggers Fluorescence Resonance EnergyTransfer (FRET) to the acceptor antibody, resulting in a specific emissionsignal at 665 nm from the acceptor. This specific signal is proportional to thedegree of interaction mediated by BCL-XL DEGRADER-2 between VHL and BCL-XL.This homogeneous assay is simple to perform and requires no washing steps.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/product.template\/20289224\/19013550-84c6-427b-a961-9cbf1af8ff95.png?access_token=cd9d7b6d-f159-4ed9-a40a-32b8fd42f52f\" class=\"img img-fluid o_we_custom_image\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"margin:12.0pt 0cm;text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eTable 1. Reagents and Storage Conditions\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:none;\" border=\"1\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003cthead\u003e\u003ctr\u003e\n\u003cth style=\"border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e100TESTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e500TESTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e2500TESTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e10000TESTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eTag1-VHL protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e40μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e200μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e800μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eTag2- BCL-XL protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e40μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e200μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e800μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eBCL-XL DEGRADER-2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e0.3 mM\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e5μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e20μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e200μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eAnti-Tag1 Eu dye antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e250μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1000μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eAnti-Tag2 AC antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e5μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e25μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e125μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e500μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2 mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10 mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e40 mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\u003c\/figure\u003e\u003cp style=\"margin:12.0pt 0cm;text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e*Reagents should be avoided from repeated freeze-thaw cycles. Aliquot upon first thaw and store under recommended conditions. Valid for one year.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"500T","offer_id":42332758278219,"sku":"UA086019-500T","price":2180.0,"currency_code":"USD","in_stock":true},{"title":"2500T","offer_id":42332758310987,"sku":"UA086019-2500T","price":8175.0,"currency_code":"USD","in_stock":true},{"title":"10000T","offer_id":42332758343755,"sku":"UA086019-10000T","price":21800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/955e8259abbe4154a604bba05e44709e.png?v=1788123818"},{"product_id":"unione-tr-fret-human-cd40cd40l-binding-kit-ua086020","title":"UniOne® TR-FRET Human CD40\/CD40L Binding Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan style=\"font-size:11.0pt\"\u003eStore the kit at -80°C or below.When stored properly, the reagents remain stable until the expiration dateprinted on the label. Once thawed, the CD40 and CD40L stock solutions canundergo one additional freeze-thaw cycle; the labeled antibody solutions canundergo one additional freeze-thaw cycle. To avoid repeated freeze-thaw cycles,it is recommended to aliquot the remaining stock solutions into single-usecontainers for storage at -80°C. Thawed dilutions and Detection Buffer can bestored at 2-8°C.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-size:11.0pt\"\u003eThis kit utilizes HomogeneousTime-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) technology tomeasure the interaction between CD40 and CD40L. This method enables simple andrapid high-throughput screening of inhibitors and blocking antibodies.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:11.0pt\"\u003eAs illustrated, the interactionbetween CD40 and CD40L is detected using an Eu-labeled Anti-Tag1 antibody(TR-FRET donor) and an Acceptor-labeled Anti-Tag2 antibody (TR-FRET acceptor).The binding of CD40 to CD40L brings the donor and acceptor antibodies intoproximity. Excitation of the donor antibody then triggers FRET to the acceptorantibody, resulting in a specific emission signal at 665 nm. The intensity ofthis specific signal is proportional to the degree of CD40\/CD40L interaction.This homogeneous assay is simple to perform and requires no washing steps.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/product.template\/20289284\/7dcfdacd-6947-435f-98a8-b5a5dc2232e1.png?access_token=96c15f41-5c7e-41dc-a494-67158b97013a\" class=\"img img-fluid o_we_custom_image\" style=\"width: 50%;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"margin:12.0pt 0cm;text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eTable 1. Kit Components and Storage Conditions\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:none;\" border=\"1\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003cthead\u003e\u003ctr\u003e\n\u003cth style=\"border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e100 TESTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e500 TESTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e2500 TESTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e10000 TESTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eTag1-CD40 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e4μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e20μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e100μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eTag2-CD40L protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e4μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e20μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e100μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eSelicrelumab (12.5μM)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e3μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e200μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eAnti-Tag1 Eu dye antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e250μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eAnti-Tag2 Acceptor antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e12.5×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e40μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e200μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e4mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e40mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\u003c\/figure\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"100T","offer_id":43090354110539,"sku":"UA086020-100T","price":730.0,"currency_code":"USD","in_stock":true},{"title":"500T","offer_id":42332758376523,"sku":"UA086020-500T","price":2180.0,"currency_code":"USD","in_stock":true},{"title":"2500T","offer_id":42332758409291,"sku":"UA086020-2500T","price":8175.0,"currency_code":"USD","in_stock":true},{"title":"10000T","offer_id":42332758442059,"sku":"UA086020-10000T","price":21800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/93ea1bb24437414fb7cbcffc9df38448.png?v=1788123820"},{"product_id":"unione-tr-fret-human-bcl6crbn-protac-binding-kit-ua086021","title":"UniOne® TR-FRET Human BCL6\/CRBN PROTAC Binding Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan style=\"font-size:11.0pt;color:#0F1115\"\u003eStore the kit at -80°C or below. When storedappropriately, the reagents remain stable until the expiration date marked onthe label. Once thawed, BCL6 and CRBN stock solutions can undergo oneadditional freeze-thaw cycle; labeled antibody solutions can undergo oneadditional freeze-thaw cycle. To avoid repeated freeze-thaw cycles, it isrecommended to aliquot the remaining stock solutions into single-use containersfor storage at -80°C. Thawed diluents and Detection Buffer can be stored at2-8°C.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-size:11.0pt;color:#0F1115\"\u003eThis kit uses Homogeneous Time-Resolved Fluorescence(TR-FRET) technology to measure the molecular glue ARV-393 and testcompound-mediated interaction between the DDB1\/CRBN complex and BCL6. Thismethod enables simple and rapid high-throughput screening of small moleculescapable of mediating the interaction between the DDB1\/CRBN complex and BCL6.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:11.0pt;color:#0F1115\"\u003eAs shown, the interaction between DDB1\/CRBN and BCL6 isdetected using a Eu-labeled Anti-Tag1 Antibody (TR-FRET donor) and anAcceptor-labeled Anti-Tag2 Antibody (TR-FRET acceptor). As the molecular glueARV-393 mediates the interaction between DDB1\/CRBN and BCL6, the donor andacceptor antibodies are brought into proximity. Excitation of the donorantibody triggers Fluorescence Resonance Energy Transfer (FRET) to the acceptorantibody, resulting in a specific emission signal at 665 nm. This specificsignal is proportional to the degree of interaction mediated by ARV-393 betweenDDB1\/CRBN and BCL6. This homogeneous assay is simple to perform and requires nowashing steps.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/product.template\/20289289\/9244b132-962e-4998-8ab0-699b7ea1602d.png?access_token=1e46193c-b79e-4f03-aa69-29ed8eac8258\" class=\"img img-fluid o_we_custom_image\" style=\"width: 50%;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"background-color:white;margin:12.0pt 0cm;text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:#0F1115;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eTable 1. Kit Components and Storage Conditions\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:none;\" border=\"1\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003cthead\u003e\u003ctr\u003e\n\u003cth style=\"border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e100 TESTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e500 TESTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e2500 TESTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e10000 TESTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003eStorage Temperature\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eTag1-DDB1\/CRBN protein,\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e250μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1000μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eTag2- BCL6 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e250μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1000μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eARV-393 (100 μM)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e5μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e25μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e125μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e500μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eAnti-Tag1 Eu dye antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e250μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1000μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eAnti-Tag2 Ac antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e20×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e25μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e125μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e625μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2500μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2ml\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10ml\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e40mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\u003c\/figure\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"500T","offer_id":42332758474827,"sku":"UA086021-500T","price":2180.0,"currency_code":"USD","in_stock":true},{"title":"2500T","offer_id":42332758507595,"sku":"UA086021-2500T","price":8175.0,"currency_code":"USD","in_stock":true},{"title":"10000T","offer_id":42332758540363,"sku":"UA086021-10000T","price":21800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/f4a2ef4aa23b45c4a1c14ee42d5f2556.png?v=1788123824"},{"product_id":"unione-tr-fret-human-vav1crbn-protac-binding-kit-ua086022","title":"UniOne® TR-FRET Human VAV1\/CRBN PROTAC Binding Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-80°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.0pt;\"\u003eThe kit employs homogeneous time-resolved fluorescence (TR-FRET) technology to measure the interaction between the DDB1\/CRBN complex and VAV1 mediated by the molecular glue MRT-6160 or test compounds. This method provides a high-throughput format for the simple and rapid detection of small molecules capable of mediating the interaction between the DDB1\/CRBN complex and VAV1.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.0pt;\"\u003eAs shown, the interaction between DDB1\/CRBN and VAV1 is detected using a Eu-labeled anti-Tag1 antibody (TR-FRET donor) and an Accepter-labeled anti-Tag2 antibody (TR-FRET acceptor). Due to the molecular glue MRT-6160 mediating the interaction between DDB1\/CRBN and VAV1, the donor and acceptor antibodies are brought into close proximity. Excitation of the donor antibody triggers fluorescence resonance energy transfer (FRET) to the acceptor antibody, causing the acceptor antibody to specifically emit a signal at a wavelength of 665 nm. This specific signal is proportional to the extent of the interaction mediated by MRT-6160 between DDB1\/CRBN and VAV1. This homogeneous assay is simple to perform and requires no washing steps.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"img img-fluid o_we_custom_image\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/product.template\/20289303\/93cfcbbd-4d52-449c-bdbf-e96f48c6043b.png?access_token=11f4906d-31b8-409f-b7d3-21d46539effe\" alt=\"\"\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;margin-left:49.3pt;width:509.8pt;\" border=\"0\" cellspacing=\"0\" cellpadding=\"0\" width=\"680\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height:28.75pt;\"\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:solid windowtext 1.0pt;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:137.9pt;\" width=\"184\"\u003e\u003cp style=\"layout-grid-mode:both;text-align:center;text-autospace:ideograph-numeric ideograph-other;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;layout-grid-mode:both;'\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border-left:none;border:solid windowtext 1.0pt;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:66.0pt;\" width=\"88\"\u003e\u003cp style=\"layout-grid-mode:both;text-align:center;text-autospace:ideograph-numeric ideograph-other;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;layout-grid-mode:both;'\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border-left:none;border:solid windowtext 1.0pt;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:58.65pt;\" width=\"78\"\u003e\u003cp style=\"layout-grid-mode:both;text-align:center;text-autospace:ideograph-numeric ideograph-other;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;layout-grid-mode:both;' lang=\"EN-US\"\u003e\u003cstrong\u003e100T\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border-left:none;border:solid windowtext 1.0pt;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:58.65pt;\" width=\"78\"\u003e\u003cp style=\"layout-grid-mode:both;text-align:center;text-autospace:ideograph-numeric ideograph-other;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;layout-grid-mode:both;' lang=\"EN-US\"\u003e\u003cstrong\u003e500T\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border-left:none;border:solid windowtext 1.0pt;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:58.65pt;\" width=\"78\"\u003e\u003cp style=\"layout-grid-mode:both;text-align:center;text-autospace:ideograph-numeric ideograph-other;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;layout-grid-mode:both;' lang=\"EN-US\"\u003e\u003cstrong\u003e2500T\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border-left:none;border:solid windowtext 1.0pt;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:58.65pt;\" width=\"78\"\u003e\u003cp style=\"layout-grid-mode:both;text-align:center;text-autospace:ideograph-numeric ideograph-other;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;layout-grid-mode:both;' lang=\"EN-US\"\u003e\u003cstrong\u003e10000T\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border-left:none;border:solid windowtext 1.0pt;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:71.3pt;\" width=\"95\"\u003e\u003cp style=\"layout-grid-mode:both;text-align:center;text-autospace:ideograph-numeric ideograph-other;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;layout-grid-mode:both;'\u003e\u003cstrong\u003eStorage Temperature\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:28.75pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:137.9pt;\" width=\"184\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003eTag1-DDB1\/CRBN protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:66.0pt;\" width=\"88\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:58.65pt;\" width=\"78\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e5μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:58.65pt;\" width=\"78\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e20μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:58.65pt;\" width=\"78\"\u003e\u003cp style=\"line-height:115%;text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;line-height:115%;' lang=\"EN-US\"\u003e100μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:58.65pt;\" width=\"78\"\u003e\u003cp style=\"line-height:115%;text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;line-height:115%;' lang=\"EN-US\"\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:71.3pt;\" width=\"95\"\u003e\u003cp style=\"line-height:115%;text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;line-height:115%;' lang=\"EN-US\"\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:28.75pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:137.9pt;\" width=\"184\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003eTag2- VAV1 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:66.0pt;\" width=\"88\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:58.65pt;\" width=\"78\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e5μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:58.65pt;\" width=\"78\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e20μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:58.65pt;\" width=\"78\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e100μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:58.65pt;\" width=\"78\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:71.3pt;\" width=\"95\"\u003e\u003cp style=\"line-height:115%;text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;line-height:115%;' lang=\"EN-US\"\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:28.75pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:137.9pt;\" width=\"184\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003eMRT-6160 （200μM）\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:66.0pt;\" width=\"88\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:58.65pt;\" width=\"78\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e5μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:58.65pt;\" width=\"78\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:58.65pt;\" width=\"78\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:58.65pt;\" width=\"78\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e200μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:71.3pt;\" width=\"95\"\u003e\u003cp style=\"line-height:115%;text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;line-height:115%;' lang=\"EN-US\"\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:28.75pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:137.9pt;\" width=\"184\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003eAnti-Tag1 Eu antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:66.0pt;\" width=\"88\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e50×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:58.65pt;\" width=\"78\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:58.65pt;\" width=\"78\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:58.65pt;\" width=\"78\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e250μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:58.65pt;\" width=\"78\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e1000μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:71.3pt;\" width=\"95\"\u003e\u003cp style=\"line-height:115%;text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;line-height:115%;' lang=\"EN-US\"\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:28.75pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:137.9pt;\" width=\"184\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003eAnti-Tag2 Ac antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:66.0pt;\" width=\"88\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:58.65pt;\" width=\"78\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e5μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:58.65pt;\" width=\"78\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e25μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:58.65pt;\" width=\"78\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e125μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:58.65pt;\" width=\"78\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e500μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:71.3pt;\" width=\"95\"\u003e\u003cp style=\"line-height:115%;text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;line-height:115%;' lang=\"EN-US\"\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:29.25pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:29.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:137.9pt;\" width=\"184\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:29.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:66.0pt;\" width=\"88\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e10×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:29.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:58.65pt;\" width=\"78\"\u003e\u003cp style=\"line-height:115%;text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;line-height:115%;' lang=\"EN-US\"\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:29.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:58.65pt;\" width=\"78\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e2mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:29.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:58.65pt;\" width=\"78\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e10mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:29.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:58.65pt;\" width=\"78\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e40mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:29.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:71.3pt;\" width=\"95\"\u003e\u003cp style=\"line-height:115%;text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;line-height:115%;' lang=\"EN-US\"\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp style=\"line-height:115%;margin-top:3.0pt;text-indent:39.6pt;\"\u003e\u003cspan style=\"font-size:10.0pt;letter-spacing:-.05pt;line-height:115%;\"\u003eNote: Aliquot immediately after the first thaw and store at the recommended storage temperature. Avoid storage after dilution and repeated freeze-thaw cycles.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"500T","offer_id":42332758605899,"sku":"UA086022-500T","price":2180.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":42332758573131,"sku":"UA086022-100T","price":730.0,"currency_code":"USD","in_stock":true},{"title":"2500T","offer_id":42332758638667,"sku":"UA086022-2500T","price":8175.0,"currency_code":"USD","in_stock":true},{"title":"10000T","offer_id":42332758671435,"sku":"UA086022-10000T","price":21800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_7bcf027e-71a9-4b69-8264-1ff1e277a148.png?v=1788519787"},{"product_id":"unione-tr-fret-human-crbn-ligand-screening-assay-kit-ua086023","title":"UniOne® TR-FRET Human CRBN Ligand Screening Assay Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003ePerson\u003c\/span\u003e\u003cspan\u003eCereblon (CRBN) protein\u003c\/span\u003e\u003cspan\u003eLigand\u003c\/span\u003e\u003cspan\u003eThe binding assay kit uses homogeneous time-resolved fluorescence technology (TR-FRET), which can be used for the detection and screening of CRBN inhibitors or ligands. It is a high-throughput screening method that enables rapid testing of CRBN inhibitors or ligands, with the basic principle shown in the figure below.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eEu\u003c\/span\u003e\u003cspan\u003eLabeled assay reagent (\u003c\/span\u003e\u003cspan\u003eAnti-Tag1-Eu\u003c\/span\u003e\u003cspan\u003e) specifically recognizes and binds to\u003c\/span\u003e\u003cspan\u003eTag1-CRBN\/DDB1\u003c\/span\u003e\u003cspan\u003eprotein, while the receptor-labeled thalidomide (\u003c\/span\u003e\u003cspan\u003eThalidomide-Ac\u003c\/span\u003e\u003cspan\u003e) combines with\u003c\/span\u003e\u003cspan\u003eCRBN\u003c\/span\u003e\u003cspan\u003e, bringing\u003c\/span\u003e\u003cspan\u003eEu\u003c\/span\u003e\u003cspan\u003eand\u003c\/span\u003e\u003cspan\u003eAc\u003c\/span\u003e\u003cspan\u003einto close proximity. Under external light excitation, energy resonance transfer occurs between the donor and acceptor, and by detecting the signal intensity at\u003c\/span\u003e\u003cspan\u003e665nm\u003c\/span\u003e\u003cspan\u003ewavelength, one can determine\u003c\/span\u003e\u003cspan\u003ethe binding of\u003c\/span\u003e\u003cspan\u003eCRBN\/DDB1\u003c\/span\u003e\u003cspan\u003ewith\u003c\/span\u003e\u003cspan\u003eThalidomide-Ac.\u003c\/span\u003e\u003cspan\u003eIn the reaction system, the added\u003c\/span\u003e\u003cspan\u003eCRBN\u003c\/span\u003e\u003cspan\u003eligand or\u003c\/span\u003e\u003cspan\u003ePROTAC\u003c\/span\u003e\u003cspan\u003ecompetitively binds to\u003c\/span\u003e\u003cspan\u003eCEBN\/DDB1,\u003c\/span\u003e\u003cspan\u003ecausing the signal to decrease, and the ligand or\u003c\/span\u003e\u003cspan\u003ePROTAC\u003c\/span\u003e\u003cspan\u003eAs the concentration increases, the signal decreases. This homogeneous assay is easy to perform and requires no washing steps.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/0aceabf0-49f0-43b5-bee5-38a1eea29b09.png\" style=\"margin-left:30px; width:685px; height:auto; vertical-align:middle;\"\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003ctable border=\"1\" style=\"border-collapse:collapse;width:auto;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eComponent\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eConcentration\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e100T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e2500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e10000T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eStorage Temperature\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eThalidomide\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10 mM in DMSO\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e25\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag1-CRBN\/DDB1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003e0×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e25\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e125\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eThalidomide-Ac\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e25\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e125\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAnti-Tag1-Eu\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e250μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1000μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e40\u003c\/span\u003e\u003cspan\u003e0μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003emL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e40\u003c\/span\u003e\u003cspan\u003emL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan\u003eNote: All\u003c\/span\u003e\u003cspan\u003eafter first thawing\u003c\/span\u003e\u003cspan\u003eimmediately\u003c\/span\u003e\u003cspan\u003edivide into aliquots\u003c\/span\u003e\u003cspan\u003eand\u003c\/span\u003e\u003cspan\u003estore\u003c\/span\u003e\u003cspan\u003eat the recommended storage temperature\u003c\/span\u003e\u003cspan\u003e,\u003c\/span\u003e\u003cspan\u003eAvoid storing after dilution and repeated freeze-thaw cycles.\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e,\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eReagent Preparation\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e.1\u003c\/span\u003e\u003cspan\u003eBefore use, allow all reagents to thaw at room temperature (equilibrate at room temperature for at least 30 minutes). The reaction volume in a 384‑well plate is 20 µL.\u003c\/span\u003e\u003cspan\u003emu\u003c\/span\u003e\u003cspan\u003eL (reagent volumes for the reaction system are shown in the table). Calculate the required reaction volume before preparation and prepare accordingly. The following formulation is for reference only, using 500 reactions as an example.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eTable 1\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003cspan\u003eReagent preparation\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eReagent name\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eConfiguration\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eμL per test well\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003etake\u003c\/span\u003e\u003cspan\u003e2 mL 10\u003c\/span\u003e\u003cspan\u003etimes\u003c\/span\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003cspan\u003eJoin\u003c\/span\u003e\u003cspan\u003e18 mL deionized water, dilute\u003c\/span\u003e\u003cspan\u003eExplanation\u003c\/span\u003e\u003cspan\u003eTo\u003c\/span\u003e\u003cspan\u003e1×, mixed\u003c\/span\u003e\u003cspan\u003eEvenly prepared\u003c\/span\u003e\u003cspan\u003eUse\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eStandard substance\u003c\/span\u003e\u003cspan\u003e\/\u003c\/span\u003e\u003cspan\u003eSample to be tested\u003c\/span\u003e\u003cspan\u003eDilution\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eUse diluted to\u003c\/span\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003etimes\u003c\/span\u003e\u003cspan\u003ePrepare the samples to be tested and the standards using the assay buffer. If the stock solution of the sample is…\u003c\/span\u003e\u003cspan\u003eDMSO\u003c\/span\u003e\u003cspan\u003e, it is recommended to maintain the system within\u003c\/span\u003e\u003cspan\u003eDMSO\u003c\/span\u003e\u003cspan\u003eThe content is consistent, and the maximum does not exceed\u003c\/span\u003e\u003cspan\u003e2%\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003cspan\u003eStandard dilutions are shown in the table below.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag1-CRBN\/DDB1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003etake\u003c\/span\u003e\u003cspan\u003e25 μL of Tag1-CRBN\/DDB1 stock solution, diluted with 1× Detection Buffer to a final volume of 2.5 mL,\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eMix thoroughly and set aside.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eThalidomide-Ac\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003etake\u003c\/span\u003e\u003cspan\u003e25 μL of thalidomide-ac stock solution, diluted with 1× detection buffer to a final volume of 2.5 mL,\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eMix thoroughly and set aside.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAnti-Tag1-Eu\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003etake\u003c\/span\u003e\u003cspan\u003e50 μL Anti-Tag1 Eu\u003c\/span\u003e\u003cspan\u003eOriginal solution, add\u003c\/span\u003e\u003cspan\u003e1× Detection buffer 2.45 mL,\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e \u003c\/span\u003e\u003cspan\u003eMix thoroughly and set aside.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan\u003e1.2\u003c\/span\u003e\u003cspan\u003eStandard Reference Material Preparation Table\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eIn the reagent kit\u003c\/span\u003e\u003cspan\u003eThalidomide\u003c\/span\u003e\u003cspan\u003eThe standard is\u003c\/span\u003e\u003cspan\u003e10 mM DMSO\u003c\/span\u003e\u003cspan\u003eMother solution. Please prepare the total amount of standard according to the experimental conditions; the following information is for reference only.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eUse diluted to\u003c\/span\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003etimes\u003c\/span\u003e\u003cspan\u003ePrepare the samples to be tested and the standards using the assay buffer. If the stock solution of the sample is…\u003c\/span\u003e\u003cspan\u003eDMSO\u003c\/span\u003e\u003cspan\u003e, it is recommended to maintain the system within\u003c\/span\u003e\u003cspan\u003eDMSO\u003c\/span\u003e\u003cspan\u003eConsistent content,\u003c\/span\u003e\u003cspan\u003e(\u003c\/span\u003e\u003cspan\u003eThe detection buffer does not contain\u003c\/span\u003e\u003cspan\u003eDMSO\u003c\/span\u003e\u003cspan\u003e, please add according to the dilution of the standard or sample to be tested into\u003c\/span\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003cspan\u003edetection buffer\u003c\/span\u003e\u003cspan\u003eDMSO)\u003c\/span\u003e\u003cspan\u003eand the maximum concentration should not exceed\u003c\/span\u003e\u003cspan\u003e2%\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eTable\u003c\/span\u003e\u003cspan\u003e2.\u003c\/span\u003e\u003cspan\u003eStandard Preparation Table\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eThalidomide\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003ePreparation Concentration (nM)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eThalidomide\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eConcentration (nM)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003ePreparation Method\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e120000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e30000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1 μL 10\u003c\/span\u003e\u003cspan\u003em\u003c\/span\u003e\u003cspan\u003eM\u003c\/span\u003e\u003cspan\u003estock solution\u003c\/span\u003e\u003cspan\u003e+ 82.3 μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e40000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10 μL\u003c\/span\u003e\u003cspan\u003e①\u003c\/span\u003e\u003cspan\u003e+ 20 μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e3\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e13333.3\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3333.33\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10 μL\u003c\/span\u003e\u003cspan\u003e②\u003c\/span\u003e\u003cspan\u003e+ 20 μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4444.4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1111.11\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10 μL\u003c\/span\u003e\u003cspan\u003e③\u003c\/span\u003e\u003cspan\u003e+ 20 μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1481.5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e370.37\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10 μL\u003c\/span\u003e\u003cspan\u003e④\u003c\/span\u003e\u003cspan\u003e+20 μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e6\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e493.8\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e123.46\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10 μL\u003c\/span\u003e\u003cspan\u003e⑤\u003c\/span\u003e\u003cspan\u003e+20 μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e7\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e164.6\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e41.15\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10μL\u003c\/span\u003e\u003cspan\u003e⑥\u003c\/span\u003e\u003cspan\u003e+20μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e8\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e54.9\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e13.72\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10μL\u003c\/span\u003e\u003cspan\u003e⑦\u003c\/span\u003e\u003cspan\u003e+20μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e9\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e18.3\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4.57\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10μL\u003c\/span\u003e\u003cspan\u003e⑧\u003c\/span\u003e\u003cspan\u003e+20μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e10\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e6.1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1.52\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10μL\u003c\/span\u003e\u003cspan\u003e⑨\u003c\/span\u003e\u003cspan\u003e+20μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eBlank\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003ctable border=\"1\" style=\"border-collapse:collapse;width:auto;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003ePositive drug standard curve\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eSample to be tested\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNegative control well (NC)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eEu control well\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eStep one\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eGradient dilution\u003c\/span\u003e\u003cspan\u003eThalidomide\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eGradient dilution of the sample to be tested\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"4\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e15μL 1\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd\u003c\/span\u003e\u003cspan\u003e5μL 1\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003cspan\u003eAnti-Tag1 Eu antibody\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5 μL Tag1-CRBN\/DDB1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eSeal the plate,\u003c\/span\u003e\u003cspan\u003e1000 rpm\u003c\/span\u003e\u003cspan\u003e, centrifuge\u003c\/span\u003e\u003cspan\u003efor 1 minute\u003c\/span\u003e\u003cspan\u003emix well,\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eincubate at room temperature\u003c\/span\u003e\u003cspan\u003efor 1\u003c\/span\u003e\u003cspan\u003e0 minutes\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"2\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eStep two\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10μL Thalidomide-Ac + Anti-Tag1-Eu 1:1 Mix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"4\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eSeal the plate,\u003c\/span\u003e\u003cspan\u003e1000 rpm\u003c\/span\u003e\u003cspan\u003e, centrifuge\u003c\/span\u003e\u003cspan\u003efor 1 minute\u003c\/span\u003e\u003cspan\u003emix well,\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eincubate at room temperature\u003c\/span\u003e\u003cspan\u003efor 120 minutes\u003c\/span\u003e\u003cspan\u003e, read the plate\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003e2\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eand\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eSample addition and controls\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.1\u003c\/span\u003e\u003cspan\u003eSamples to be tested\u003c\/span\u003e\u003cspan\u003e:\u003c\/span\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003egradient dilution\u003c\/span\u003e\u003cspan\u003esamples to be tested\u003c\/span\u003e\u003cspan\u003e,\u003c\/span\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003cspan\u003eTag1-CRBN\/DDB1\u003c\/span\u003e\u003cspan\u003eworking solution,\u003c\/span\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003cspan\u003eThalidomide-Ac\u003c\/span\u003e\u003cspan\u003eworking solution,\u003c\/span\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003cspan\u003eAnti-Tag1-Eu\u003c\/span\u003e\u003cspan\u003eworking solution (\u003c\/span\u003e\u003cspan\u003eT\u003c\/span\u003e\u003cspan\u003ehalidomide-Ac\u003c\/span\u003e\u003cspan\u003ecan be mixed with\u003c\/span\u003e\u003cspan\u003eAnti-Tag1-Eu\u003c\/span\u003e\u003cspan\u003ein proportion\u003c\/span\u003e\u003cspan\u003eAfter mixing, add it sequentially to a 384-well plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.2\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003ePositive drug standard curve:\u003c\/span\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL gradient dilution\u003c\/span\u003e\u003cspan\u003eThalidomide\u003c\/span\u003e\u003cspan\u003e,\u003c\/span\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003cspan\u003eTag1-CRBN\/DDB1\u003c\/span\u003e\u003cspan\u003eWorking fluid,\u003c\/span\u003e\u003cspan\u003e5 μL Thalidomide-Ac\u003c\/span\u003e\u003cspan\u003eWorking fluid,\u003c\/span\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003cspan\u003eAnti-Tag1-Eu\u003c\/span\u003e\u003cspan\u003eWorking fluid.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e.3\u003c\/span\u003e\u003cspan\u003eNegative\u003c\/span\u003e\u003cspan\u003eComparison\u003c\/span\u003e\u003cspan\u003eHole (NC)\u003c\/span\u003e\u003cspan\u003e:\u003c\/span\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer instead\u003c\/span\u003e\u003cspan\u003eTo be tested\u003c\/span\u003e\u003cspan\u003eSample;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.4\u003c\/span\u003e\u003cspan\u003eEu control hole\u003c\/span\u003e\u003cspan\u003e: 1\u003c\/span\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eAdd μL of 1× Detection buffer\u003c\/span\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL of diluted Anti-Tag1 Eu antibody.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAfter adding all samples, centrifuge, seal with a plate cover, and incubate at room temperature for 2 hours.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e3.\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eDetection\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eDetection on a plate reader compatible with TR-FRET\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003cspan\u003eThe excitation wavelength is 320\/340 nm, with detection at 620 nm and 665 nm.\u003c\/span\u003e\u003cspan\u003esend\u003c\/span\u003e\u003cspan\u003eWavelength of the radiation.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e[Result Calculation]\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1)\u003c\/span\u003e\u003cspan\u003eCalculate the signal value\u003c\/span\u003e\u003cspan\u003e(Ratio)\u003c\/span\u003e\u003cspan\u003e: Multiply the 665nm fluorescence signal by the 620nm fluorescence signal, then multiply by 10000\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eRatio\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e=\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e(665\/620)\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003cspan\u003e10000\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003cspan\u003eCalculate CV (%)\u003c\/span\u003e\u003cspan\u003e:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eCV (%) = Standard Deviation \/ Mean Ratio × 100%\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e[Data Example]\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe following data cannot replace the data obtained in experiments; it is only for illustration, and results may\u003c\/span\u003e\u003cspan\u003evary depending on the plate reader\u003c\/span\u003e\u003cspan\u003eand\u003c\/span\u003e\u003cspan\u003einstrument.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e(1) Standard Curve*\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAs shown in the chart, due to\u003c\/span\u003e\u003cspan\u003ethalidomide\u003c\/span\u003e\u003cspan\u003estandards competing with\u003c\/span\u003e\u003cspan\u003ethalidomide-Ac\u003c\/span\u003e\u003cspan\u003efor binding to\u003c\/span\u003e\u003cspan\u003eCRBN\/DDB1\u003c\/span\u003e\u003cspan\u003e, the signal value decreases.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/e3a9635f-a3f6-4d76-90a0-4b4a0bbf0b03.jpeg\" style=\"margin-left:30px; width:497px; height:auto; vertical-align:middle;\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e(2) Binding activity and inhibitor validation*\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThalidomide-Ac binding to CRBN\/DDB1 was verified. The results show that when the protein concentration remains constant, the detection signal increases with increasing concentrations of Thalidomide-Ac. Lenalidomide, Pomalidomide, and PROTAC BET Degrader-1 can compete with Thalidomide-Ac for binding to CRBN\/DDB1.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/8c29295e-0163-47ba-9b46-6e8afcea6de9.jpeg\" style=\"margin-left:30px; width:496px; height:auto; vertical-align:middle;\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e*\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eData are for illustrative purposes only; recommended\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eMicroplate (384-well plate, white, shallow wells)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"500T","offer_id":42332758769739,"sku":"UA086023-500T","price":2180.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":43090712395851,"sku":"UA086023-100T","price":730.0,"currency_code":"USD","in_stock":true},{"title":"2500T","offer_id":42332758802507,"sku":"UA086023-2500T","price":5489.0,"currency_code":"USD","in_stock":true},{"title":"10000T","offer_id":42332758835275,"sku":"UA086023-10000T","price":13098.83,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_52cca4b2-a1f1-41d9-ae77-18f12f4ba331.png?v=1788894011"},{"product_id":"unione-tr-fret-human-pd1pd-l1-binding-kit-ua086026","title":"UniOne® TR-FRET Human PD1\/PD-L1 Binding Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-80℃\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003eThe assay kit utilizes homogeneous time-resolved fluorescence (TR-FRET) technology to measure the interaction between PD1 and PD-L1. This method enables simple, rapid, and high-throughput screening of inhibitors and antibody blockers.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAs shown in the figure, the interaction between PD1 and PD-L1 is detected using an Eu-labeled anti-Tag1 antibody (TR-FRET donor) and an Ac-labeled anti-Tag2 antibody (TR-FRET acceptor). The binding of PD1 and PD-L1 brings the donor and acceptor antibodies into proximity, allowing the excitation of the donor antibody to trigger fluorescence resonance energy transfer (FRET) to the acceptor antibody, resulting in a specific emission signal at 665 nm. Pembrolizumab, as a positive control, blocks the interaction between PD1 and PD-L1, preventing FRET signal generation. The stronger the blocking effect of the screened drug on the PD1\/PD-L1 interaction, the lower the signal. The specific signal is proportional to the extent of the PD1\/PD-L1 interaction. This homogeneous assay is simple to perform and requires no washing steps.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/d8a622aa-fff7-42b1-9d10-036eedd16c60.png\" style=\"margin-left:30px; width:329px; height:auto; vertical-align:middle;\" alt=\"\"\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"text-align:left;\"\u003e \u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:1px solid #000000;width:auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003e100T\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003e500T\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003e2500T\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003e10000T\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003eStorage Temperature\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003ePembrolizumab\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e5μM\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e5μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e200μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eTag1-PD-L1 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e5μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e20μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e100μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eTag2- PD1 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e5μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e20μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e100μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eAnti-Tag1 Eu antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e50×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e250μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e1000μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eAnti-Tag2 Ac antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e12.5×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e40μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e200μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e1000μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e4000μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e10×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e2mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e10mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e40mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp style=\"text-align:left;\"\u003e\u003cbr\u003e\u003cspan style=\"color:#000000;\"\u003eNote: Aliquot immediately upon first thaw and store at the recommended temperature. Avoid storage after dilution and repeated freeze-thaw cycles.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003e1. Reagent Preparation\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003e1.1 Thaw all reagents to room temperature before use (equilibrate at room temperature for at least 30 min). The reaction volume for the 384-well shallow plate is 20 μL (reagent volumes for the reaction system are shown in the table). Calculate the required volume for the experiment before preparation and prepare as needed; the following preparation is for reference only, using 500 reactions as an example.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"color:#000000\"\u003eTable 1. Reagent Preparation\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003eReagent Name\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003ePreparation\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003eVolume per Assay Well (μL)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003eTag1-PD-L1 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:justify\"\u003e\u003cspan style=\"color:#000000\"\u003eTake 20 μL of Tag1-PD-L1 protein stock solution and dilute to 2 mL with 1× Detection buffer,\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003emix well and set aside.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003eTag2- PD1 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:justify\"\u003e\u003cspan style=\"color:#000000\"\u003eTake 20 μL of Tag2-PD1 protein stock solution and dilute to 2 mL with 1× Detection buffer,\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003emix well and set aside.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003eMix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan style=\"color:#000000\"\u003eTake 50 μL of Anti-Tag1 Eu antibody stock solution and add 2.45 mL of 1× Detection buffer,\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003e mix well and set aside;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan style=\"color:#000000\"\u003eTake 200 μL of Anti-Tag2 Ac antibody stock solution and add 2.3 mL of 1× Detection buffer,\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003e mix well and set aside;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan style=\"color:#000000\"\u003eAfter diluting Anti-Tag1 Eu antibody and Anti-Tag2 Ac antibody separately, mix them at a 1:1 ratio\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003emix well and set aside.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e10\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003ePembrolizumab\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003eAccording to the reaction system, dilute the compound with 1× Detection buffer to 10 times the final concentration in the system to serve as a positive control. For example, if the final concentration in the system is 10 nM, the dilution concentration should be 100 nM.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003eSimultaneously, use DMSO diluted with 1× Detection buffer as the negative control well.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan style=\"color:#000000\"\u003e1.2 \u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eGradient Dilution of Test Samples\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003eUsing \u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003ePembrolizumab\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e as an example, the diluent is 1× Detection buffer. To minimize the impact of matrix effect interference, it is recommended to dilute with a solution having the same matrix as the test sample; adjust the test sample according to its actual concentration.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"color:#000000\"\u003eTable 2. Gradient Dilution of Positive Drug \u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003ePembrolizumab\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e (Adjust according to actual conditions)\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003ePembrolizumab\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003e Final Concentration (nM)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003ePembrolizumab\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003e Preparation Concentration (nM)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003ePreparation Method\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e①\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e10\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e100\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e1\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eμL\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e 5\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eμ\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eM \u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003ePembrolizumab\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e+49\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eμL\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e \u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e1× Detection buffer \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e②\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e50\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e25\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eμL\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e \u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e①\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e +25\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eμL\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e \u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e③\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e2.5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e25\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e25\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eμL\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e \u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e②\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e +25\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eμL\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e \u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e④\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e1.25\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e12.5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e25\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eμL\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e \u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e③\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e +25\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eμL\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e \u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e⑤\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e0.63\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e6.3\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e25\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eμL\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e \u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e④\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e +25\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eμL\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e \u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e⑥\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e0.31\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e3.1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e25\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eμL\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e \u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e⑤\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e +25\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eμL\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e \u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e⑦\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e0.16\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e1.6\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e25\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eμL\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e \u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e⑥\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e +25\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eμL\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e \u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e⑧\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e0.08\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e0.8\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e25\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eμL\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e \u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e⑦\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e +25\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eμL\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e \u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e⑨\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e0.04\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e0.4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e25\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eμL\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e \u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e⑧\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e +25\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eμL\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e \u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e⑩\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e0.02\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e0.2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e25\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eμL\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e \u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e⑩\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e +25\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eμL\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e \u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003eBlank\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e25\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eμL\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e \u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003eEu+Ac\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003e2. Sample Addition and Controls\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003e2.1 Sample addition order for assay wells: Add 2 μL of test sample, 4 μL of Tag2-PD1 protein working solution, 4 μL of Tag1-PD-L1 protein working solution, and 10 μL of mixed Mix sequentially into the 384-well shallow plate;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003e2.2 \u003c\/span\u003e\u003cspan\u003eMaximum Control\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e: 2 μL of 1× Detection buffer, 4 μL of Tag2-PD1 protein working solution, 4 μL of Tag1-PD-L1 protein working solution, and 10 μL of mixed Mix;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003e2.3 Quality Control Well (NC): 10 μL of 1× Detection buffer plus 10 μL of Mix.\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse:collapse;width:auto;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003eMaximum Control\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003eSample\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003eNC\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003eStep 1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e1× Detection Buffer (containing DMSO)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003eGradient diluted test sample\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"4\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e10uL 1× Detection Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"2\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e4μL Tag2-PD1 protein working solution\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"2\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003eIncubate for 10 min\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"2\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003eStep 2\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"2\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e4μL Tag1-PD-L1 protein working solution\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e10μL Mix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003eStep 3\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003eSeal with plate sealer, incubate at room temperature for 2 h\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan style=\"color:#000000\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e3. \u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003eDetection\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003eDetect on a microplate reader compatible with TR-FRET. Excitation wavelength is 320\/340 nm, and emission wavelengths are 620 nm and 665 nm. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e[Result Calculation]\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003e1) Calculate the signal value (Ratio): Divide the 665 nm fluorescence signal by the 620 nm fluorescence signal, then multiply by 10,000.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"color:#000000\"\u003eRatio\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e \u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e=\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e \u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e(665\/620)\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e ×\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e10000\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003e2) Calculate \u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eNet signal\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e based on the signal value: \u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"color:#000000\"\u003eNet signal\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e = (Std-NC)\/NC×100\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003e3) Calculate CV (%):\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"color:#000000\"\u003eCV (%) = Standard Deviation\/Mean Ratio × 100%\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"color:#000000\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"color:#000000\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e[Data Example]\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003eThe following data cannot replace the data obtained in experiments and is only for illustration; results may vary depending on the plate reader.\u003c\/span\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"color:#000000\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/d19d787a-f70e-4b4f-a89a-1ca93486c163.png\" style=\"margin-left:30px; width:366px; height:auto; vertical-align:middle;\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/37286bb3-96dc-470d-9450-7ca26b1c3fe0.jpeg\" style=\"margin-left:30px; width:364px; height:auto; vertical-align:middle;\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cspan style=\"color:#000000\"\u003e \u003c\/span\u003e\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"color:#000000\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003eNote: Recommended \u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003emicroplate (384-well plate, white, shallow well)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"500T","offer_id":42332760801355,"sku":"UA086026-500T","price":2180.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":43091042664523,"sku":"UA086026-100T","price":730.0,"currency_code":"USD","in_stock":true},{"title":"2500T","offer_id":43091042697291,"sku":"UA086026-2500T","price":8175.0,"currency_code":"USD","in_stock":true},{"title":"10000T","offer_id":43091042730059,"sku":"UA086026-10000T","price":21800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_2e3458af-050c-443b-ad71-223023780588.png?v=1790560808"},{"product_id":"unione-tr-fret-human-camp-hirange-detection-kit-ua086053","title":"UniOne® TR-FRET Human cAMP Hirange Detection Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003e-80\u003c\/span\u003e\u003cspan\u003e℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003eThe kit employs homogeneous time-resolved fluorescence technology (TR-FRET) to precisely measure cAMP produced by cells. This method can rapidly detect changes in cAMP accumulation upon inhibition or activation of Gs- or Gi-mediated signaling pathways.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAs shown in the figure, the Eu-labeled anti-cAMP antibody (TR-FRET donor) and the Ac-labeled cAMP (TR-FRET acceptor) bind directly, bringing the donor and acceptor antibodies into close proximity. Excitation of the donor antibody triggers fluorescence resonance energy transfer (FRET) to the acceptor antibody, resulting in a specific emission signal at 665 nm. When cAMP is present in cell samples or standard curves, it competes with the Ac-labeled cAMP for binding to the Eu-labeled anti-cAMP antibody, thereby reducing the FRET signal. This specific signal is proportional to the extent of interaction between the Eu-labeled anti-cAMP antibody and the Ac-labeled cAMP. The homogeneous assay is easy to perform and requires no washing steps.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/8ceb77b6-336b-4d0d-a764-d8fb096f9ebb.png\" style=\"margin-left:30px; width:485px; height:auto; vertical-align:middle;\"\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003ctable border=\"1\" style=\"border-collapse:collapse;width:auto;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eComponent\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eConcentration\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e250T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e1000T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e5000T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e20000T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eStorage Temperature\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003ecAMP\u003c\/span\u003e\u003cspan\u003eCalibrator\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4mM\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e200 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80\u003c\/span\u003e\u003cspan\u003e℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAnti-cAMP Eu antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e12.5 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e250 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1000 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80\u003c\/span\u003e\u003cspan\u003e℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003ecAMP-Ac\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e12.5 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e250 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1000 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80\u003c\/span\u003e\u003cspan\u003e℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eLysis \u0026amp; Detect Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5 mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20 mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100 mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e400 mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80\u003c\/span\u003e\u003cspan\u003e℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eStimulation Buffer (without IBMX)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5 mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20 mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100 mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e400 mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80\u003c\/span\u003e\u003cspan\u003e℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan\u003eCaution: After the first thaw, immediately aliquot and store at the recommended temperature. Avoid storing diluted samples or subjecting them to repeated freeze-thaw cycles.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"250T","offer_id":42332760834123,"sku":"UA086053-250T","price":470.0,"currency_code":"USD","in_stock":true},{"title":"1000T","offer_id":42332760866891,"sku":"UA086053-1000T","price":1100.0,"currency_code":"USD","in_stock":true},{"title":"5000T","offer_id":42332760899659,"sku":"UA086053-5000T","price":3000.0,"currency_code":"USD","in_stock":true},{"title":"20000T","offer_id":42332760932427,"sku":"UA086053-20000T","price":7500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_6a9e7f78-59fa-4935-a1c7-e8d04ffafd84.png?v=1788523226"},{"product_id":"unione-tr-fret-human-pcsk9-ldlr-assay-kit-ua086058","title":"UniOne® TR-FRET Human PCSK9\/LDLR Assay Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-80℃\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003eThe kit employs homogeneous time-resolved fluorescence technology (TR-FRET) for measuring the interaction between Human PCSK9 and Human LDLR. This method enables simple, rapid, and high-throughput screening of inhibitors and antibody blockers.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAs shown in the figure, the interaction between PCSK9 and LDLR is detected using an Eu-labeled anti-Tag1 antibody (TR-FRET donor) and an Ac-labeled Tag2 antibody (TR-FRET acceptor). The binding of PCSK9 to LDLR brings the donor and acceptor antibodies into proximity, allowing the excitation of the donor antibody to trigger fluorescence resonance energy transfer (FRET) to the acceptor antibody, resulting in a specific emission signal at 665 nm. Screened compounds, such as Pep 2-8, block the binding of PCSK9 to LDLR, preventing FRET signal generation. The stronger the blocking effect of the screened compound on PCSK9 and LDLR, the lower the signal. The signal is proportional to the degree of interaction between PCSK9 and LDLR. No washing steps are required.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"img img-fluid o_we_custom_image\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/product.template\/20294079\/3244ab13-4543-46f8-b61b-3273c58d5e71.png?access_token=73a7957c-7b87-411f-9acb-a0c0d6c84fb9\" alt=\"\"\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003eHere is the translated table with all formatting preserved:```html\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse:collapse;width:auto;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eComponent\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eConcentration\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e100T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e2500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e10000T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eStorage Temperature\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag1-LDLR protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag2-PCSK9 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAnti-Tag1 Eu antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e250μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1000μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAnti-Tag2 Ac antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e12.5×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e40μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e200μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e14mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e60mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e240mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e```\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003e1. Reagent Preparation\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003e1.1 Thaw all reagents at room temperature before use (allow at least 30 minutes for equilibration). The reaction system for the 384-well plate is 20μL (reagent volumes are shown in the table below). Calculate the required volume before preparation and prepare accordingly. The following preparation is for reference only, using 500 reactions as an example.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003eTable 1. Reagent Preparation\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:1px solid #000000;width:auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"background-color:#8BAAD2;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eReagent Name\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003ePreparation\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eVolume per Well (μL)\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eSample Dilution\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan\u003eDilute the sample to 1× using detection buffer. If the sample stock is in DMSO, ensure the DMSO content in the system is consistent and does not exceed 2%.\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eTag1-LDLR protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan\u003eTake 20μL of Tag1-LDLR protein stock, dilute to 2mL with 1× Detection buffer, and mix well.\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eTag2-PCSK9 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\n\u003cspan\u003eTake 20μL of Tag2-PCSK9 protein stock, dilute to 2mL with 1× Detection buffer, \u003c\/span\u003e\u003cstrong\u003eand mix well.\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eAntibody Mix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\n\u003cspan\u003eTake 50μL of Anti-Tag1 Eu antibody stock, add 2.45mL of 1× Detection buffer, \u003c\/span\u003e\u003cstrong\u003eand mix;\u003c\/strong\u003e\u003cspan\u003e take 200μL of Anti-Tag2 Ac antibody stock, add 2.3mL of 1× Detection buffer, and mix. Combine Anti-Tag1 Eu antibody and Anti-Tag2 Ac antibody in a 1:1 volume ratio to obtain the Antibody Mix.\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003e1.2 Gradient Dilution of Test Samples\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eTaking Pep 2-8 as an example, the diluent is 1× Detection buffer. To reduce the interference of matrix effects, it is recommended to use a solution with the same matrix as the test sample for dilution. The dilution of the test sample should be adjusted according to the actual concentration.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003eTable 2. \u003c\/span\u003e\u003cspan\u003ePep 2-8 Preparation Table\u003c\/span\u003e\u003cspan style=\"color:#000000;\"\u003e (Adjust according to actual conditions)\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:1px solid #000000;width:auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"background-color:#8BAAD2;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003ePep 2-8 Preparation Concentration (nM)\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003ePep 2-8 Final Concentration (nM)\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003ePreparation Method\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"margin-right:8px;\"\u003e1\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e200000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e20000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e4μL 1mM Pep 2-8 + 16μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"margin-right:8px;\"\u003e2\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e66666.67\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e6666.67\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e7μL ① + 14μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"margin-right:8px;\"\u003e3\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e22222.22\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e2222.22\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e7μL ② + 14μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"margin-right:8px;\"\u003e4\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e7407.41\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e740.74\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e7μL ③ + 14μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"margin-right:8px;\"\u003e5\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e2469.14\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e246.91\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e7μL ④ + 14μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"margin-right:8px;\"\u003e6\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e823.05\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e82.30\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e7μL ⑤ + 14μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"margin-right:8px;\"\u003e7\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e274.35\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e27.43\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e7μL ⑥ + 14μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"margin-right:8px;\"\u003e8\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e91.45\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e9.14\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e7μL ⑦ + 14μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"margin-right:8px;\"\u003e9\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e30.48\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e3.05\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e7μL ⑧ + 14μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eBlank\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e7μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003e2. Sample Loading and Controls\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.1 Test samples: Add 2μL gradient-diluted test samples, 4μL Tag2-PCSK9 protein, 4μL Tag1-LDLR protein working solution, and 10μL Antibody Mix sequentially into a 384-well shallow plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.2 Blank control wells (Blank): Replace test samples with 2μL 1× Detection buffer.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003e2.3 Eu control wells (NC): Add 10μL 1× Detection buffer and 10μL Antibody Mix.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003eAfter all samples are loaded, centrifuge, cover with sealing film, and incubate at room temperature for 2 hours.\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:1px solid #000000;width:auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eTest Samples\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eBlank Control Wells (Blank)\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eEu Control Wells (NC)\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" rowspan=\"3\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eStep 1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eGradient-diluted test samples\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" rowspan=\"5\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e+10μL Antibody Mix\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"2\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e4μL Tag2-PCSK9 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"2\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eSeal plate, mix by centrifugation at 1000rpm for 1min,\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eincubate at room temperature for 10min\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" rowspan=\"3\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eStep 2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"2\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e4μL Tag1-LDLR protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"2\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL Antibody Mix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"3\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eSeal plate, mix by centrifugation at 1000rpm for 1min, incubate at room temperature for 120min, then read plate\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"margin-right:8px;\"\u003e3、\u003c\/span\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003eDetection\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003ePerform detection on a TR-FRET-compatible microplate reader. Excitation wavelength is 320\/340 nm, with emission wavelengths detected at 620 nm and 665 nm.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#1655A5;\"\u003e\u003cstrong\u003e【Result Calculation】\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1) Calculate the signal value (Ratio): Divide the 665 nm fluorescence signal by the 620 nm fluorescence signal and multiply by 10,000.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan\u003eRatio = (665\/620) ×10000\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2) Calculate the Net signal based on the signal value:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan\u003eNet signal = (Std-NC)\/NC×100\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3) Calculate CV (%):\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan\u003eCV (%) = Standard Deviation\/Mean Ratio × 100%\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#1655A5;\"\u003e\u003cstrong\u003e【Data Example】\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003eThe following data cannot replace the data obtained in experiments and is only provided as an example. Results may vary depending on the plate reader instrument.\u003c\/span\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"img img-fluid o_we_custom_image\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/product.template\/20294079\/9581bf5e-bc6e-471e-bbd4-734056440288.png?access_token=bb8e0be4-8d88-41e7-813b-6e8bb33ac8a4\" alt=\"\"\u003e\u003cimg style=\"height:auto;margin-left:30px;max-width:100%;vertical-align:middle;\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/f82dd0f7-d5f3-49db-81e5-7a0e332aeb82.emf\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003eNote: Recommended microplate (384-well plate, white, shallow well)\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"500T","offer_id":42332762046539,"sku":"UA086058-500T","price":2180.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":42332762013771,"sku":"UA086058-100T","price":730.0,"currency_code":"USD","in_stock":true},{"title":"2500T","offer_id":42332762079307,"sku":"UA086058-2500T","price":8175.0,"currency_code":"USD","in_stock":true},{"title":"10000T","offer_id":42332762112075,"sku":"UA086058-10000T","price":21800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_c38feb01-db81-4863-815c-51dce9fdc25c.png?v=1788523317"},{"product_id":"unione-tr-fret-human-btkcrbn-protac-binding-kit-ua086079","title":"UniOne® TR-FRET Human BTK\/CRBN PROTAC Binding Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-80℃\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eThe assay kit utilizes homogeneous time-resolved fluorescence (TR-FRET) technology to measure the interaction between the DDB1\/CRBN complex and BTK mediated by molecular glue SJF620 and test compounds. This method enables a high-throughput format for simple and rapid detection of small molecules that can mediate the interaction between the DDB1\/CRBN complex and BTK.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eAs shown, the interaction between DDB1\/CRBN and BTK is detected using a Eu-labeled anti-Tag1 antibody (TR-FRET donor) and an Accepter-labeled anti-Tag2 antibody (TR-FRET acceptor). Since molecular glue SJF620 mediates the interaction between DDB1\/CRBN and BTK, the donor antibody and acceptor antibody come into proximity, allowing the excitation of the donor antibody to trigger fluorescence resonance energy transfer (FRET) to the acceptor antibody, resulting in a specific emission signal at 665 nm. This specific signal is proportional to the extent of interaction mediated by SJF620 between DDB1\/CRBN and BTK. The homogeneous assay is simple to perform and requires no washing steps.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e                                           \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/10183402-e97a-4597-8b19-a4b26f9fc349.png\" style=\"margin-left:30px; width:531px; height:auto; vertical-align:middle;\" alt=\"\"\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003ctable border=\"1\" style=\"border-collapse:collapse;width:auto;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eComponent\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eConcentration\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e100T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e2500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e10000T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eStorage Temperature\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag1-DDB1\/CRBN protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e8\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e40\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e200\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e800\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag2- \u003c\/span\u003e\u003cspan\u003eBTK\u003c\/span\u003e\u003cspan\u003e protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e500\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2m\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eSJF620\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1m\u003c\/span\u003e\u003cspan\u003eM\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e200μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAnti-Tag1 Eu antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e250μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003em\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAnti-Tag2 \u003c\/span\u003e\u003cspan\u003eAc\u003c\/span\u003e\u003cspan\u003e antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e12.5\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003cspan\u003e0μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e200μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003em\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003cspan\u003em\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e40mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e[Experimental Procedure and Operation]\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e.\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eReagent Preparation\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e.1 \u003c\/span\u003e\u003cspan\u003eMelt all reagents at room temperature before use (equilibrate at room temperature for at least 30 min). The reaction volume for the 384-well shallow well plate is 20\u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan\u003eL (reagent volumes for the reaction system are shown in the table). Calculate the required volume for the experiment prior to preparation and prepare as needed; the following preparation is for reference only, using 500 tests as an example.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eTable 1\u003c\/span\u003e\u003cspan\u003e. \u003c\/span\u003e\u003cspan\u003eReagent Preparation\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eReagent Name\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003ePreparation\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eVolume per Detection Well (μL)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eTake\u003c\/span\u003e\u003cspan\u003e 2 mL 10\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003cspan\u003e Detection buffer 1\u003c\/span\u003e\u003cspan\u003e and add\u003c\/span\u003e\u003cspan\u003e 18 mL deionized water, di\u003c\/span\u003e\u003cspan\u003elute\u003c\/span\u003e\u003cspan\u003e to\u003c\/span\u003e\u003cspan\u003e 1×, mix\u003c\/span\u003e\u003cspan\u003e well and\u003c\/span\u003e\u003cspan\u003e set aside\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eSJF620\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eAccording to the reaction system, dilute the compound to the desired concentration using\u003c\/span\u003e\u003cspan\u003e 1× Detection buffer\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eEnsure consistent DMSO concentration across all detection wells\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag1-DDB1\/CRBN protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eTake\u003c\/span\u003e\u003cspan\u003e 40\u003c\/span\u003e\u003cspan\u003eμL \u003c\/span\u003e\u003cspan\u003eTag1-DDB1\/CRBN protein\u003c\/span\u003e\u003cspan\u003e stock solution, dilute to 2 mL with 1× Detection buffer,\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e mix well and set aside for later use.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag2- \u003c\/span\u003e\u003cspan\u003eBTK\u003c\/span\u003e\u003cspan\u003e protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eTake\u003c\/span\u003e\u003cspan\u003e 100\u003c\/span\u003e\u003cspan\u003eμL \u003c\/span\u003e\u003cspan\u003eTag2- \u003c\/span\u003e\u003cspan\u003eBTK\u003c\/span\u003e\u003cspan\u003e protein\u003c\/span\u003e\u003cspan\u003e stock solution, dilute to 2 mL with 1× Detection buffer,\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e mix well and set aside for later use.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAntibody Mix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eTake\u003c\/span\u003e\u003cspan\u003e 50μL Anti-Tag1 Eu antibody \u003c\/span\u003e\u003cspan\u003estock solution, add\u003c\/span\u003e\u003cspan\u003e 2.45 mL of 1× Detection buffer,\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e \u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003emix well;\u003c\/span\u003e\u003cspan\u003e take\u003c\/span\u003e\u003cspan\u003e 200μL Anti-Tag2 Ac antibody \u003c\/span\u003e\u003cspan\u003estock solution, add\u003c\/span\u003e\u003cspan\u003e 2.3 mL of 1× Detection buffer,\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e \u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003emix well\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e;\u003c\/span\u003e\u003cspan\u003e mix the two solutions 1:1 to prepare Antibody Mix.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan\u003e1.2 \u003c\/span\u003e\u003cspan\u003eGradient Dilution of Test Samples\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eUsing SJF620 as an example, the diluent is 1× Detection buffer. To minimize matrix effect interference, it is recommended to dilute with a solution matching the sample matrix; adjust the test samples according to actual concentrations.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eTable\u003c\/span\u003e\u003cspan\u003e2. \u003c\/span\u003e\u003cspan\u003ePositive Control Drug Gradient Dilution\u003c\/span\u003e\u003cspan\u003e (Adjust according to actual conditions)\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eSJF620 Final Concentration (nM)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eSJF620 Prepared Concentration (nM)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003ePreparation Method\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10000.00\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100000.00\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003eμL \u003c\/span\u003e\u003cspan\u003e1 mM\u003c\/span\u003e\u003cspan\u003e Stock Solution\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e18\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2500.00\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e25000.00\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL \u003c\/span\u003e\u003cspan\u003e①\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e15\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e3\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e625.00\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e6250.00\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL \u003c\/span\u003e\u003cspan\u003e②\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e15\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e156.25\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1562.50\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL \u003c\/span\u003e\u003cspan\u003e③\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e15\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e39.06\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e390.63\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5μL \u003c\/span\u003e\u003cspan\u003e④\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e15\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e6\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e9.77\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e97.66\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5μL \u003c\/span\u003e\u003cspan\u003e⑤\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e15\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e7\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2.44\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e24.41\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5μL \u003c\/span\u003e\u003cspan\u003e⑥\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e15\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e8\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e0.61\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e6.10\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5μL \u003c\/span\u003e\u003cspan\u003e⑦\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e15\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e9\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e0.15\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1.53\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5μL\u003c\/span\u003e\u003cspan\u003e⑧\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e15\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e10\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e0.04\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e0.38\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5μL \u003c\/span\u003e\u003cspan\u003e⑨\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e15\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan\u003eBlank\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e15\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003e2\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e.\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eSample Addition and Controls\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse:collapse;width:auto;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003ePositive Control Standard Curve\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eTest Samples\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eBlank\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNC\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eGradient Diluted SJF620\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eGradient Diluted Test Samples\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"4\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003eμL 1\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003cspan\u003e Detection buffer\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eAntibody Mix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4μL Tag1- CRBN protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4μL Tag2-\u003c\/span\u003e\u003cspan\u003eBTK \u003c\/span\u003e\u003cspan\u003eprotein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10μL Antibody Mix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"4\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eSeal the plate wells with a sealing film\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e,\u003c\/span\u003e\u003cspan\u003eincubate at room temperature for 2 hours;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan\u003e2.1 \u003c\/span\u003e\u003cspan\u003eTest Samples: 2μL gradient diluted test samples, 4μL Tag1-DDB1\/CRBN protein working solution, 4μL Tag2-\u003c\/span\u003e\u003cspan\u003eBTK \u003c\/span\u003e\u003cspan\u003eprotein working solution, 10μL mixed Antibody Mix (Tag2-\u003c\/span\u003e\u003cspan\u003eBTK \u003c\/span\u003e\u003cspan\u003eprotein can be mixed with Antibody Mix in proportion before addition), added sequentially into the 384-well shallow well plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.2 \u003c\/span\u003e\u003cspan\u003e Positive Control Standard Curve: 2μL gradient diluted SJF620, 4μL Tag1-DDB1\/CRBN protein working solution, 4μL Tag2-\u003c\/span\u003e\u003cspan\u003eBTK \u003c\/span\u003e\u003cspan\u003eprotein working solution, 10μL Antibody Mix.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e.3 \u003c\/span\u003e\u003cspan\u003eBlank: 2μL 1× Detection buffer replaces test samples;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.4 \u003c\/span\u003e\u003cspan\u003eNegative Control Wells (NC): 10μL 1× Detection buffer plus 10μL Antibody Mix.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAfter adding all samples, centrifuge, cover with a sealing film, and incubate at room temperature for 2 hours.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e3.\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eDetection\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eDetect using a microplate reader compatible with TR-FRET. Excitation wavelength is 320\/340nm, and emission wavelengths detected are 620nm and 665nm. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e[Result Calculation]\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1)\u003c\/span\u003e\u003cspan\u003e Calculate Signal Value (Ratio): Divide the 665nm fluorescence signal by the 620nm fluorescence signal, then multiply by 10000.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eRatio\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e=\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e(665\/620)\u003c\/span\u003e\u003cspan\u003e ×\u003c\/span\u003e\u003cspan\u003e10000\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2)\u003c\/span\u003e\u003cspan\u003e Calculate Net signal based on signal values:\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eNet signal\u003c\/span\u003e\u003cspan\u003e = (Std-\u003c\/span\u003e\u003cspan\u003eNC\u003c\/span\u003e\u003cspan\u003e)\/\u003c\/span\u003e\u003cspan\u003eNC\u003c\/span\u003e\u003cspan\u003e×100\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3)\u003c\/span\u003e\u003cspan\u003e Calculate CV (%):\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eCV (%) = Standard Deviation\/Mean Ratio × 100%\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e[Data Example]\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe following data cannot replace data obtained from experiments and is for illustration only. Results may vary depending on the microplate reader used.\u003c\/span\u003e  \u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/88659b1f-2bc4-402d-a705-621fe33d4dcb.png\" style=\"margin-left:30px; width:376px; height:auto; vertical-align:middle;\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003eNote: Recommended microplate (384-well plate, white, shallow well)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"100T","offer_id":42332763258955,"sku":"UA086079-100T","price":730.0,"currency_code":"USD","in_stock":true},{"title":"500T","offer_id":42332763291723,"sku":"UA086079-500T","price":2180.0,"currency_code":"USD","in_stock":true},{"title":"2500T","offer_id":42332763324491,"sku":"UA086079-2500T","price":8175.0,"currency_code":"USD","in_stock":true},{"title":"10000T","offer_id":42332763357259,"sku":"UA086079-10000T","price":21800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_31ca40a8-ec3f-4a55-98a8-454432c32aa7.png?v=1788523449"},{"product_id":"unione-tr-fret-human-bafftaci-binding-kit-ua086120","title":"UniOne® TR-FRET Human BAFF\/TACI Binding Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-80℃\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003eThe kit employs homogeneous time-resolved fluorescence (TR-FRET) technology to measure the interaction between Human BAFF and Human TACI. This method enables simple, rapid, and high-throughput screening of inhibitors and antibody blockers.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAs shown in the figure, the interaction between BAFF and TACI is detected using an Eu-labeled anti-Tag1 antibody (TR-FRET donor) and an acceptor-labeled Tag2 antibody (TR-FRET acceptor). The binding of BAFF and TACI brings the donor and acceptor antibodies into close proximity, allowing the excitation of the donor antibody to trigger fluorescence resonance energy transfer (FRET) to the acceptor antibody, resulting in a specific 665 nm emission signal. The positive control drug Tabalumab blocks the binding of BAFF and TACI, preventing FRET signal generation. The stronger the inhibitory effect of the screened drug on BAFF and TACI interaction, the lower the signal. The signal intensity is proportional to the degree of BAFF-TACI interaction. No washing steps are required.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/8fd2d3d9-9dac-4696-8027-2c28247962a0.png\" style=\"margin-left: 30px; max-width: 100%; height: auto; vertical-align: middle;\" alt=\"\"\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003ctable border=\"1\" style=\"border-collapse:collapse;width:auto;\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border-top:1px solid #000000;border-left:1px solid #000000;border-right:1px solid #000000;border-bottom:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003eComponent\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border-top:1px solid #000000;border-right:1px solid #000000;border-bottom:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003eConcentration\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border-top:1px solid #000000;border-right:1px solid #000000;border-bottom:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003e100T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border-top:1px solid #000000;border-right:1px solid #000000;border-bottom:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003e500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border-top:1px solid #000000;border-right:1px solid #000000;border-bottom:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003e2500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border-top:1px solid #000000;border-right:1px solid #000000;border-bottom:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003e10000T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border-top:1px solid #000000;border-right:1px solid #000000;border-bottom:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003eStorage Temperature\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border-left:1px solid #000000;border-right:1px solid #000000;border-bottom:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003eTag1-BAFF protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border-right:1px solid #000000;border-bottom:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e100 ×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border-right:1px solid #000000;border-bottom:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e5μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border-right:1px solid #000000;border-bottom:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e20μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border-right:1px solid #000000;border-bottom:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e100μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border-right:1px solid #000000;border-bottom:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border-right:1px solid #000000;border-bottom:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border-left:1px solid #000000;border-right:1px solid #000000;border-bottom:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003eTag2-\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e \u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eTACI protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border-right:1px solid #000000;border-bottom:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e100 ×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border-right:1px solid #000000;border-bottom:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e5μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border-right:1px solid #000000;border-bottom:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e20μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border-right:1px solid #000000;border-bottom:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e100μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border-right:1px solid #000000;border-bottom:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border-right:1px solid #000000;border-bottom:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border-left:1px solid #000000;border-right:1px solid #000000;border-bottom:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003eTabalumab\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border-right:1px solid #000000;border-bottom:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e25μM\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border-right:1px solid #000000;border-bottom:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e5μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border-right:1px solid #000000;border-bottom:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e15μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border-right:1px solid #000000;border-bottom:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e75μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border-right:1px solid #000000;border-bottom:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e300μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border-right:1px solid #000000;border-bottom:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border-top:1px solid #000000;border-left:1px solid #000000;border-right:1px solid #000000;border-bottom:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003eEu-anti-Tag1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border-top:1px solid #000000;border-right:1px solid #000000;border-bottom:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e50 ×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border-top:1px solid #000000;border-right:1px solid #000000;border-bottom:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border-top:1px solid #000000;border-right:1px solid #000000;border-bottom:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border-top:1px solid #000000;border-right:1px solid #000000;border-bottom:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e250μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border-top:1px solid #000000;border-right:1px solid #000000;border-bottom:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e1000μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border-top:1px solid #000000;border-right:1px solid #000000;border-bottom:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border-top:1px solid #000000;border-left:1px solid #000000;border-right:1px solid #000000;border-bottom:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003eAc-anti-Tag2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border-top:1px solid #000000;border-right:1px solid #000000;border-bottom:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e12.5 ×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border-top:1px solid #000000;border-right:1px solid #000000;border-bottom:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e40μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border-top:1px solid #000000;border-right:1px solid #000000;border-bottom:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e200μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border-top:1px solid #000000;border-right:1px solid #000000;border-bottom:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e1mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border-top:1px solid #000000;border-right:1px solid #000000;border-bottom:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e4mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border-top:1px solid #000000;border-right:1px solid #000000;border-bottom:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border-top:1px solid #000000;border-left:1px solid #000000;border-right:1px solid #000000;border-bottom:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border-top:1px solid #000000;border-right:1px solid #000000;border-bottom:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e10 ×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border-top:1px solid #000000;border-right:1px solid #000000;border-bottom:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border-top:1px solid #000000;border-right:1px solid #000000;border-bottom:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e2mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border-top:1px solid #000000;border-right:1px solid #000000;border-bottom:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e10mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border-top:1px solid #000000;border-right:1px solid #000000;border-bottom:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e40mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border-top:1px solid #000000;border-right:1px solid #000000;border-bottom:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"color:#000000\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"color:#000000\"\u003eAttention: After the first thaw, aliquot immediately and store at the recommended temperature. Avoid storing diluted samples or subjecting them to repeated freeze-thaw cycles.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003e1. Reagent Preparation\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003e1.1 Thaw all reagents at room temperature before use (allow at least 30 minutes for equilibration). The reaction system for the 384-well plate is 20μL (reagent volumes per reaction are shown in the table below). Calculate the required volume before preparation and prepare accordingly. The following preparation is for reference only, using 500 reactions as an example.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003eTable 1. Reagent Preparation\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-bottom:1px solid #000000;border-collapse:collapse;border-left:1px solid #000000;border-right:1px solid #000000;border-top:1px solid #000000;width:auto;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"background-color:#8BAAD2;border-bottom:1px solid #000000;border-right:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003eReagent Name\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border-bottom:1px solid #000000;border-right:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003ePreparation\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border-bottom:1px solid #000000;border-right:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003eVolume per Well (μL)\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-bottom:1px solid #000000;border-right:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:1px solid #000000;border-right:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:justify;\"\u003e\u003cspan style=\"color:#000000;\"\u003eTake 2 mL of 10× Detection buffer and add 18 mL of deionized water to dilute to 1×. Mix well and set aside.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:1px solid #000000;border-right:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e-\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-bottom:1px solid #000000;border-right:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eTag1-BAFF protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:1px solid #000000;border-right:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:justify;\"\u003e\u003cspan style=\"color:#000000;\"\u003eTake 20 μL of 100× Tag1-BAFF protein stock solution and add to 1.98 mL of 1× Detection buffer to dilute to 1×. Mix well and set aside.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:1px solid #000000;border-right:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e4μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-bottom:1px solid #000000;border-right:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eTag2-TACI protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:1px solid #000000;border-right:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:justify;\"\u003e\u003cspan style=\"color:#000000;\"\u003eTake 20 μL of 100× Tag2-TACI protein stock solution and add to 1.98 mL of 1× Detection buffer to dilute to 1×. Mix well and set aside.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:1px solid #000000;border-right:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e4μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-bottom:1px solid #000000;border-right:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eDetection Reagent Mix\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom:1px solid #000000;border-right:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\n\u003cp style=\"text-align:justify;\"\u003e\u003cspan style=\"color:#000000;\"\u003eTake 50 μL of 50× Eu-anti-Tag1 stock solution and add to 2.45 mL of 1× Detection buffer to dilute to 2.5 mL. Mix well.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify;\"\u003e\u003cspan style=\"color:#000000;\"\u003eTake 200 μL of 12.5× Ac-anti-Tag2 stock solution and add to 2.30 mL of 1× Detection buffer to dilute to 2.5 mL. Mix well.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify;\"\u003e\u003cspan style=\"color:#000000;\"\u003eMix the two solutions at a 1:1 ratio to prepare the Detection Mix.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom:1px solid #000000;border-right:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e \u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003e1.2 Gradient Dilution of Test Samples\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003eTaking Tabalumab as an example, the dilution buffer is 1× Detection buffer. To minimize matrix interference effects, it is recommended to use a solution with the same matrix as the test sample for dilution. Adjustments should be made based on the actual concentration of the sample.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003eTable 2. Gradient Dilution of Positive Control Tabalumab (Adjust as Needed)\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-bottom:1px solid #000000;border-collapse:collapse;border-left:1px solid #000000;border-right:1px solid #000000;border-top:1px solid #000000;width:auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"background-color:#8BAAD2;border-bottom:1px solid #000000;border-right:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border-bottom:1px solid #000000;border-right:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003eTabalumab Preparation Concentration (nM)\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border-bottom:1px solid #000000;border-right:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003eTabalumab Final Concentration (nM)\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border-bottom:1px solid #000000;border-right:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003ePreparation Method\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-bottom:1px solid #000000;border-right:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e①\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:1px solid #000000;border-right:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e2500\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:1px solid #000000;border-right:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e250\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:1px solid #000000;border-right:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e3μL 25μM stock solution + 27μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd\u003e\n\u003cstrong\u003e2. Sample Loading and Controls\u003c\/strong\u003e\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003e2.1 Sample Well: Add 2μL of test sample (gradient dilution), 4μL Tag1-BAFF protein working solution, 4μL Tag2-TACI protein working solution, and 10μL detection reagent Mix sequentially into a 384-well shallow plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003e2.2 Maximum Signal Control: Add 2μL Detection buffer, 4μL Tag1-BAFF protein working solution, 4μL Tag2-TACI protein working solution, and 10μL detection reagent Mix sequentially into a 384-well shallow plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003e2.3 Negative Control: Add 10μL Detection buffer and 10μL detection reagent Mix sequentially into a 384-well shallow plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003eAfter loading all samples, centrifuge, seal with plate film, and incubate at room temperature for 2 hours.\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;width:auto;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"background-color:#8BAAD2;border-bottom:1px solid #000000;border-left:1px solid #000000;border-right:1px solid #000000;border-top:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003e \u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border-bottom:1px solid #000000;border-right:1px solid #000000;border-top:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003eSample\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border-bottom:1px solid #000000;border-right:1px solid #000000;border-top:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003eMaximum Signal Control\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border-bottom:1px solid #000000;border-right:1px solid #000000;border-top:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003eNegative Control (NC)\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-bottom:1px solid #000000;border-left:1px solid #000000;border-right:1px solid #000000;text-align:center;vertical-align:middle;\" rowspan=\"3\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eStep 1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:1px solid #000000;border-right:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e2μL Test Sample\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-right:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e2μL 1×Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-right:1px solid #000000;text-align:center;vertical-align:middle;\" rowspan=\"4\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e10μL 1×Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border-bottom:1px solid #000000;border-right:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"2\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e4μL Tag1-BAFF protein working solution\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border-bottom:1px solid #000000;border-right:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"2\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eIncubate at room temperature for 10min\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-bottom:1px solid #000000;border-left:1px solid #000000;border-right:1px solid #000000;text-align:center;vertical-align:middle;\" rowspan=\"3\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eStep 2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:1px solid #000000;border-right:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"2\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e4μL Tag2-TACI protein working solution\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border-bottom:1px solid #000000;border-right:1px solid #000000;border-top:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"3\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e10μL Detection reagent Mix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border-bottom:1px solid #000000;border-right:1px solid #000000;border-top:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"3\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eSeal with plate film, incubate at room temperature for 2h\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/tbody\u003e \u003c\/table\u003e```\u003c\/figure\u003e\u003cp\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"margin-right:8px;\"\u003e3、\u003c\/span\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003eDetection\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003ePerform detection on a TR-FRET-compatible microplate reader. The excitation wavelength is 320\/340 nm, and the emission wavelengths are 620 nm and 665 nm.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#1655A5;\"\u003e\u003cstrong\u003e【Result Calculation】\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003e1) Calculate the signal value (Ratio): Divide the 665 nm fluorescence signal by the 620 nm fluorescence signal and multiply by 10,000.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003eRatio = (665\/620) × 10,000\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003e2) Calculate the Net signal based on the signal value:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003eNet signal = (Std - NC)\/NC × 100\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003e3) Calculate CV (%):\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003eCV (%) = Standard Deviation\/Mean Ratio × 100%\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#1655A5;\"\u003e\u003cstrong\u003e【Data Example】\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003eThe following data is for illustrative purposes only and cannot replace the data obtained in experiments. Results may vary depending on the plate reader.\u003c\/span\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"img img-fluid o_we_custom_image\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/product.template\/20966904\/3a7eb968-96fa-43aa-8b78-639844198405.png?access_token=d2d859c1-8714-4c1b-ac67-3447825ddf64\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003eNote: Recommended microplate (384-well plate, white, shallow well)\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"500T","offer_id":42332765716555,"sku":"UA086120-500T","price":2180.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":42332765683787,"sku":"UA086120-100T","price":730.0,"currency_code":"USD","in_stock":true},{"title":"2500T","offer_id":42332765749323,"sku":"UA086120-2500T","price":8175.0,"currency_code":"USD","in_stock":true},{"title":"10000T","offer_id":42332765782091,"sku":"UA086120-10000T","price":21800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_5ac5c245-8703-401f-82b5-7916c1f4e69c.png?v=1788530484"},{"product_id":"unione-tr-fret-human-vcbvhl-complexbinding-kit-ua086119","title":"UniOne® TR-FRET Human VCB（VHL Complex）Binding Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-80℃\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003eThe reagent kit employs homogeneous time-resolved fluorescence technology (TR-FRET) for measuring the interaction between Human VCB (VHL\/Elongin-C\/Elongin-B Complex) and Human HIF-1α. The Tag2-Tracer is a fluorescently labeled HIF-1α peptide fragment. This method enables simple and rapid high-throughput screening of inhibitors and antibody blockers.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAs shown in the figure, the interaction between VCB and Tracer is detected using a Eu-labeled anti-Tag1 antibody (TR-FRET donor) and an Acceptor-labeled SA (TR-FRET acceptor). Since the binding of VCB and Tracer brings the donor antibody and acceptor antibody into proximity, excitation of the donor antibody triggers fluorescence resonance energy transfer (FRET) to the acceptor antibody, resulting in a specific emission signal at 665 nm. VH032 can block the binding of VCB and Tracer, preventing the generation of FRET signals. The stronger the blocking effect of the screened drug on the VCB-Tracer interaction, the lower the signal. The signal is proportional to the degree of interaction between VCB and Tracer. No washing steps are required.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/c6fc677d-95f7-49ab-b09b-c1bec72d8cb3.png\" style=\"margin-left: 30px; max-width: 100%; height: auto; vertical-align: middle;\" alt=\"\"\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003ctable border=\"1\" style=\"border-collapse:collapse;width:auto;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eComponent\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eConcentration\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e100T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e2500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e10000T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eStorage Temperature\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag1-VCB protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100 ×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag2-Tracer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100 ×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eEu-anti-Tag1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50 ×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e250μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1000μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eSA-Ac\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100 ×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e25μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e125μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e500μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1 ×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e14mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e70mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e240mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003e1. Reagent Preparation\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003e1.1 Before use, thaw all reagents at room temperature (room temperature equilibration for at least 30 min). The 384-well shallow well plate reaction system volume is 20 μL (reagent volumes for the reaction system are shown in the table); calculate the required volume before preparation and prepare as needed; the following preparation is for reference only, taking 500 times as an example.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003eTable 1. Reagent Preparation\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:1px solid #000000;width:auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"background-color:#8BAAD2;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eReagent Name\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003ePreparation\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eVolume per Detection Well μL\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eTag1-VCB protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan\u003eTake 20 μL 100 × Tag1-VCB protein stock solution, add to 1.98 mL 1×Detection buffer to dilute to 1×, mix and set aside.\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e4μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eTag2-Tracer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan\u003eTake 20 μL 100 × Tag2-Tracer stock solution, add to 1.98 mL 1×Detection buffer to dilute to 1×, mix and set aside.\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e4μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eDetection Reagent Mix\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\n\u003cp\u003e\u003cspan\u003eTake 50 μL 50× Eu-anti-Tag1 stock solution, add 2.45 mL 1×Detection buffer to dilute to 2.5 mL, mix;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eTake 25 μL 100× SA-Ac stock solution, add 1×Detection buffer 2.475 mL to dilute to 2.5 mL, mix;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eMix both 1:1, this is Detection Mix.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003e1.2 Gradient Dilution of Test Samples\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eTaking VH032 as an example, the diluent is 1× Detection buffer. To reduce the interference of matrix effects, it is recommended to use a solution with the same matrix as the test sample for dilution; and adjust the test samples according to actual concentration.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003eTable 2. \u003c\/span\u003e\u003cspan\u003ePositive Drug VH032 Gradient Dilution (Adjust According to Actual Situation)\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:1px solid #000000;width:auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"background-color:#8BAAD2;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eVH032 Prepared Concentration (nM)\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eVH032 Final Concentration (nM)\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003ePreparation Method\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e①\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e30,000.0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e300,000.0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e2μL 3mM Stock Solution + 18μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e②\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e10000.0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e100000.0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL ① +20μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e③\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e3333.3\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e33333.3\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL ② +20μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e④\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e1111.1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e11111.1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL ③ +20μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑤\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e370.4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e3703.7\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL ④ +20μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑥\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e123.5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e1234.6\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL ⑤ +20μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑦\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e41.2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e411.5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL ⑥ +20μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑧\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e13.7\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e137.2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL ⑦ +20μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑨\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e4.6\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e45.7\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL ⑧ +20μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eBlank\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e0.0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e20μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003e2. Sample Addition and Controls\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003e2.1 Sample Well (Sample): Add 2 μL test sample (gradient dilution), 4 μL Tag1-VCB protein working solution, 4 μL Tag2-Tracer working solution, 10 μL Detection Reagent Mix sequentially into the 384-well shallow well plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003e2.2 Maximum Signal Control: Add 2 μL Detection buffer, 4 μL Tag1-VCB protein working solution, 4 μL Tag2-Tracer working solution, 10 μL Detection Reagent Mix sequentially into the 384-well shallow well plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003e2.3 Negative Control (Negative control): Add 10 μL Detection buffer, 10 μL Detection Reagent Mix sequentially into the 384-well shallow well plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003eAfter adding all samples, centrifuge, seal the plate film, and incubate at room temperature for 2 hours.\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:1px solid #000000;width:auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e　\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eSample (Sample)\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eMaximum Signal Control\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eNegative Control (NC)\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" rowspan=\"3\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eStep 1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e2μL Test Sample\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e2μL 1×Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" rowspan=\"4\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL 1×Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"2\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e4μL Tag1-VCB protein Working Solution\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"2\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eIncubate at Room Temperature 10 min\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" rowspan=\"3\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eStep 2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"2\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e4μL Tag2-Tracer Working Solution\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"3\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL Detection Reagent Mix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"3\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eSeal plate with film, incubate at room temperature for 2h\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"margin-right:8px;\"\u003e3.\u003c\/span\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003eDetection\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003eDetect on a microplate reader compatible with TR-FRET. Excitation light is 320\/340 nm, detect emission wavelengths of 620 nm and 665 nm.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#1655A5;\"\u003e\u003cstrong\u003e[Result Calculation]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1) Calculate Signal Value (Ratio): Fluorescence signal at 665 nm divided by fluorescence signal at 620 nm, then multiplied by 10000.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan\u003eRatio = (665\/620) ×10000\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2) Calculate Net signal based on signal value:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan\u003eNet signal = (Std-NC)\/NC×100\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3) Calculate CV (%):\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan\u003eCV (%) = Standard Deviation\/Mean Ratio × 100%\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#1655A5;\"\u003e\u003cstrong\u003e[Data Example]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003eThe data below cannot replace data obtained from experiments, it is for demonstration purposes only; results may vary depending on the plate reader instrument.\u003c\/span\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"img img-fluid o_we_custom_image\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/product.template\/20992085\/5de11f0c-0bb4-4101-94bb-9d2ec47e2284.png?access_token=b07117e4-7211-4263-a785-a64035e93e1a\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cimg style=\"height:auto;margin-left:30px;max-width:100%;vertical-align:middle;\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/2c2b03a1-42f2-4ccb-b7e3-4f8ca73eff0f.emf\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003eNote: Recommended Microplate (384-well plate, White, Shallow Well)\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"500T","offer_id":42332766339147,"sku":"UA086119-500T","price":2180.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":42332766306379,"sku":"UA086119-100T","price":730.0,"currency_code":"USD","in_stock":true},{"title":"2500T","offer_id":42332766371915,"sku":"UA086119-2500T","price":8175.0,"currency_code":"USD","in_stock":true},{"title":"10000T","offer_id":42332766404683,"sku":"UA086119-10000T","price":21800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_f8fe438f-d895-41d3-b4b2-3ea565be98d1.png?v=1788530493"},{"product_id":"unione-tr-fret-human-il33st2-binding-kit-ua086118","title":"UniOne® TR-FRET Human IL33\/ST2 Binding Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-80℃\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003eThe reagent kit employs homogeneous time-resolved fluorescence technology (TR-FRET) for measuring the interaction between Human IL33 (Interleukin-33) and Human ST2 (also known as IL1RL1, Interleukin 1 Receptor Like 1). This method enables simple and rapid high-throughput screening of inhibitors and antibody blockers.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAs illustrated, the interaction between IL33 and ST2 is detected using Eu-labeled anti-Tag1 antibody (TR-FRET donor) and Accepter-labeled Tag2 antibody (TR-FRET acceptor). The binding of IL33 and ST2 brings the donor and acceptor antibodies into proximity, allowing the excitation of the donor antibody to trigger fluorescence resonance energy transfer (FRET) to the acceptor antibody, resulting in a specific emission signal at 665 nm. The positive control drug Itepekimab blocks the binding of IL33 and ST2, preventing FRET signal generation. The stronger the blocking effect of the screened drug on the IL33-ST2 interaction, the lower the signal. The signal is proportional to the degree of IL33-ST2 interaction. No washing steps are required.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/95cd4b5d-8f4a-40bf-85e3-be56b11af2cf.png\" style=\"margin-left: 30px; max-width: 100%; height: auto; vertical-align: middle;\" alt=\"\"\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003ctable border=\"1\" style=\"border-collapse:collapse;width:auto;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e组分\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e浓度\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e100T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e2500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e10000T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e储存温度\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag1-ST2 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100 ×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag2-\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eIL33 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100 ×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eEu-anti-Tag1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50 ×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e250μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1000μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAc-anti-Tag2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e12.5 ×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e40μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e200μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10 ×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e40mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003eHere is the translated content in English with original formatting preserved:```html\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003e1. Reagent Preparation\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003e1.1 Thaw all reagents at room temperature before use (equilibrate for at least 30 min). The reaction system for 384-well shallow plates is 20μL (reagent volumes are shown in the table below). Calculate the required volume before preparation and prepare accordingly. The following preparation is for reference only, using 500 reactions as an example.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003eTable 1. Reagent Preparation\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:1px solid #000000;width:auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"background-color:#8BAAD2;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eReagent Name\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003ePreparation\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eVolume per Well (μL)\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan\u003eTake 2 mL of 10× Detection buffer and add 18 mL deionized water to dilute to 1×. Mix well.\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e-\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eTag1-ST2 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan\u003eTake 20 μL of 100× Tag1-ST2 protein stock and add to 1.98 mL 1× Detection buffer to dilute to 1×. Mix well.\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e4μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eTag2-IL33 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan\u003eTake 20 μL of 100× Tag2-IL33 protein stock and add to 1.98 mL 1× Detection buffer to dilute to 1×. Mix well.\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e4μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eDetection Mix\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\n\u003cp\u003e\u003cspan\u003eTake 50 μL of 50× Eu-anti-Tag1 stock and add 2.45 mL 1× Detection buffer to dilute to 2.5 mL. Mix well;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eTake 200 μL of 12.5× Ac-anti-Tag2 stock and add 2.30 mL 1× Detection buffer to dilute to 2.5 mL. Mix well;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eMix the two solutions 1:1 to prepare the Detection Mix.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003e1.2 Sample Serial Dilution\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eUsing Itepekimab as an example, dilute with 1× Detection buffer. To minimize matrix interference, it is recommended to use a solution with the same matrix as the sample. Adjust dilution based on actual sample concentration.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003eTable 2. Serial Dilution of Positive Control (Itepekimab) (Adjust as needed)\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:1px solid #000000;width:auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"background-color:#8BAAD2;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003eFinal Conc. (nM)\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003ePrep. Conc. (nM)\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003ePreparation Method\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e①\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e100.000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e1000.000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e2 μL of 10 μM stock + 18 μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e②\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e25.000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"#000000;\"\u003e250.000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e5 μL of ① + 15 μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e③\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e6.250\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e62.500\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e5 μL of ② + 15 μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e④\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e1.563\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e15.625\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e5 μL of ③ + 15 μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑤\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e0.391\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e3.906\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e5 μL of ④ + 15 μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑥\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e0.098\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e0.977\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e5 μL of ⑤ + 15 μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑦\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e0.024\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e0.244\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e5 μL of ⑥ + 15 μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑧\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e0.006\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e0.061\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e5 μL of ⑦ + 15 μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eBlank\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e20 μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003e2. Sample Loading and Controls\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003e2.1 Sample wells: Add sequentially to 384-well shallow plates—2 μL diluted sample, 4 μL Tag2-IL33 protein working solution, 4 μL Tag1-ST2 protein working solution, and 10 μL Detection Mix.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003e2.2 Maximum signal control: Add sequentially—2 μL 1× Detection buffer, 4 μL Tag2-IL33 protein working solution, 4 μL Tag1-ST2 protein working solution, and 10 μL Detection Mix.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003e2.3 Negative control (NC): Add sequentially—10 μL 1× Detection buffer and 10 μL Detection Mix.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003eAfter loading, centrifuge and seal with plate film. Incubate at room temperature for 2 hours.\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:1px solid #000000;width:auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eSample\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eMax Signal Control\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eNegative Control (NC)\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" rowspan=\"3\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eStep 1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e2 μL sample\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e2 μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" rowspan=\"4\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10 μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"2\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e4 μL Tag2-IL33 protein working solution\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"2\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eIncubate at RT for 10 min\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" rowspan=\"3\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eStep 2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"2\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e4 μL Tag1-ST2 protein working solution\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"3\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10 μL Detection Mix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"3\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eSeal and incubate at RT for 2 h\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"margin-right:8px;\"\u003e3.\u003c\/span\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003eDetection\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003eRead on a TR-FRET-compatible microplate reader. Excitation wavelength: 320\/340 nm; emission wavelengths: 620 nm and 665 nm.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#1655A5;\"\u003e\u003cstrong\u003e[Data Analysis]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003e1) Calculate signal ratio (Ratio): Divide 665 nm fluorescence by 620 nm fluorescence, then multiply by 10,000.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003eRatio = (665\/620) × 10000\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003e2) Calculate Net signal:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003eNet signal = (Std - NC)\/NC × 100\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003e3) Calculate CV (%):\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003eCV (%) = Standard Deviation\/Mean Ratio × 100%\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#1655A5;\"\u003e\u003cstrong\u003e[Example Data]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003eThe following data is for illustration only and may vary depending on the plate reader used.\u003c\/span\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"img img-fluid o_we_custom_image\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/product.template\/20992086\/564e40ae-019e-4771-9e20-ffae7bccaeca.png?access_token=6e4469b0-20f3-45c0-9bb2-5afdd7506be9\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cimg style=\"height:auto;margin-left:30px;max-width:100%;vertical-align:middle;\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/3c12ca49-8089-407d-ae50-d40ddc30403b.emf\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003eNote: Recommended microplate (384-well, white, shallow)\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e```\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"500T","offer_id":42332766470219,"sku":"UA086118-500T","price":2180.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":42332766437451,"sku":"UA086118-100T","price":730.0,"currency_code":"USD","in_stock":true},{"title":"2500T","offer_id":42332766502987,"sku":"UA086118-2500T","price":8175.0,"currency_code":"USD","in_stock":true},{"title":"10000T","offer_id":42332766535755,"sku":"UA086118-10000T","price":21800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_f255baad-e4eb-4227-b887-32ad7d83a4a3.png?v=1788530495"},{"product_id":"unione-tr-fret-his-check-kit-ua086117","title":"UniOne® TR-FRET His Check kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-80℃\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003eThe kit employs homogeneous time-resolved fluorescence technology (\u003c\/span\u003e\u003cspan\u003eTR-FRET) to quantify His-tagged proteins. This kit can be used both to confirm whether the His-tag motif of a protein is recognized by anti-His antibodies and to estimate the concentration of His-tagged proteins.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAs shown in the figure, the protein with a\u003c\/span\u003e\u003cspan\u003eHis tag is labeled with the Acceptor, namely Ac-Protein His Tag, while the antibody that recognizes the His tag is labeled with Eu, i.e., Eu-anti-His. After the antibody binds to the His tag on the protein, the distance between the donor and acceptor decreases, and excitation of the donor antibody triggers fluorescence resonance energy transfer (FRET) to the acceptor antibody, causing the acceptor antibody to emit a signal at a wavelength of 665 nm. When other His-tagged proteins are present, they compete with Ac-Protein His Tag for binding to Eu-anti-His, preventing FRET signals from occurring; the higher the concentration of these other His-tagged proteins, the greater the competition with Ac-Protein His Tag.\u003c\/span\u003e\u003cspan\u003eCombine\u003c\/span\u003e\u003cspan\u003eThe stronger the Eu-anti-His binding capacity, the lower the signal. The signal is directly proportional to the binding of Ac-Protein His Tag and Eu-anti-His, and no washing steps are required.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/4133b89b-8c3c-4abc-abaa-22e56c79d8b7.png\" style=\"margin-left:30px; width:476px; height:auto; vertical-align:middle;\"\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003ctable border=\"1\" style=\"border-collapse:collapse;width:auto;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eComponent\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eConcentration\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e100T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e2500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e10000T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eStorage Temperature\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eHis Tag Control\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1mM\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eEu-anti-His\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100 ×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e250μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1000μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAc-Protein His Tag\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e40μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e200μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10 ×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e40mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003e1. Reagent Preparation\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003e1.1 Before use, thaw all reagents at room temperature (allow them to equilibrate at room temperature for at least 30 minutes). The reaction system in a 384-well plate is 20 μL (the amount of reagents for the reaction system is shown in the table below). Calculate the required volume for your experiment before preparation and prepare accordingly; the following preparation is for reference only, using 500 reactions as an example.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003eTable 1. Reagent Preparation\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:1px solid #000000;width:auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eReagent Name\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003ePreparation\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eVolume per Detection Well (μL)\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cspan\u003eTake 2 mL of 10× Detection buffer and add 18 mL of deionized water to dilute it to 1×, mix well, and set aside.\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e-\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eEu-anti-His\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cspan\u003eTake 25 μL of 100× Eu-anti-His stock solution and add it to 2.745 mL of 1× Detection buffer to dilute it to 1×, then mix well and set aside.\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e5 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eAc-Protein His Tag\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cspan\u003eTake 25 μL of the 100× Ac-Protein His Tag stock solution and add it to 2.745 mL of 1× Detection buffer to dilute to 1×, mix well, and set aside.\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e5 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003e1.2 Gradient Dilution of Samples to Be Measured\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eTaking His Tag Control as an example, the diluent is 1× Detection buffer. To reduce the impact of matrix effects, it is recommended to dilute with a solution that has the same matrix as the sample to be tested; moreover, the concentration of the sample should be adjusted according to its actual level.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003eTable 2. Gradient Dilution of Positive Control His Tag (Adjust According to Actual Conditions)\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:1px solid #000000;width:auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eFinal Concentration of His Tag Control (nM)\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003ePreparation Concentration of His Tag Control (nM)\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003ePreparation Method\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e①\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e5000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e1 μL of 1 mM stock solution + 99 μL of 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e②\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e1666.67\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e3333.33\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10 μL ① + 20 μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e③\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e555.56\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e1111.11\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10 μL ② + 20 μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e④\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e185.19\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e370.37\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10 μL ③ + 20 μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑤\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e61.73\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e123.46\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10 μL ④ + 20 μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑥\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e20.58\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e41.15\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10 μL ⑤ + 20 μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑦\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e6.86\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e13.72\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10 μL ⑥ + 20 μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑧\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e2.29\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e4.57\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10 μL ⑦ + 20 μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑨\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e0.76\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e1.52\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10 μL ⑧ + 20 μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑩\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e0.25\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e0.51\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10 μL ⑨ + 20 μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eBlank\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e0.0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e20 μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003e2. Sample Addition and Controls\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.1 Sample Wells: Add 10 μL of the sample to be tested (gradient dilution), 5 μL of Eu-anti-His working solution, and 5 μL of Ac-Protein His Tag sequentially into the 384-well plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.2 Positive Control: Add 10 μL of 1× Detection buffer, 5 μL of Eu-anti-His working solution, and 5 μL of Ac-Protein His Tag sequentially into the 384-well plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.3 Negative control: Add 15 μL of Detection buffer and 5 μL of Eu-anti-His working solution sequentially into the 384-well plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAfter all samples have been added, centrifuge, seal with a plate cover, and incubate at room temperature for 2 hours.\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable border=\"1\" style=\"border-collapse:collapse;border:1px solid #000000;width:auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e　\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eSample\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003ePositive control (maximum signal)\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eNegative control (NC)\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"3\" style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eStep 1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10 μL of sample to be tested\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10 μL of 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd rowspan=\"4\" style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e15 μL of 1× Detection buffer plus 5 μL of Eu-anti-His working solution\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"2\" style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e5 μL of Eu-anti-His working solution\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"2\" style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eIncubate at room temperature for 10 minutes\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"2\" style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eStep two\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd colspan=\"2\" style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e5 μL of Ac-Protein His Tag\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"3\" style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eSeal the plate with a membrane, incubate at room temperature for 2 hours\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"margin-right:8px;\"\u003e3.\u003c\/span\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003eDetection\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003eDetect on an ELISA reader compatible with TR-FRET. Use an excitation wavelength of 320\/340 nm, and measure emission wavelengths at 620 nm and 665 nm.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#1655A5;\"\u003e\u003cstrong\u003e[Result Calculation]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003e1) Calculate the signal value (Ratio): Multiply the 665nm fluorescence signal by the 620nm fluorescence signal, then multiply by 10000.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003eRatio = (665\/620) ×10000\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003e2) Calculate the Net signal based on the signal value:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003eNet signal = (Std-NC)\/NC×100\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003e3) Calculate CV (%):\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003eCV (%) = Standard Deviation\/Mean Ratio × 100%\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#1655A5;\"\u003e\u003cstrong\u003e[Data Example]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003eThe following data cannot replace the data obtained in experiments; it is only for illustration, and results may vary depending on the plate reader.\u003c\/span\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" class=\"image_resized\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/5fbb9c91-9745-4105-9a34-53dee3f6beca.png\" style=\"height:auto;margin-left:30px;vertical-align:middle;width:380px;\"\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable border=\"1\" style=\"border-collapse:collapse;border:1px solid #000000;width:auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003eName\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003eMolecular weight kDa\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003eHis\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003eIC50\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eHis-Peptide\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e3.328\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e10×His\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e44.29\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eHis-Protein 1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e20\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e10×His\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e1.817\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eHis-Protein 2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e49\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e10×His\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e6.671\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eHis-Protein 3\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e62\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e6×His\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e138\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eHis-Protein 4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e180\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e6×His\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e101.6\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote: Microplates are recommended (384-well plates, white, shallow wells)\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"100T","offer_id":42332766830667,"sku":"UA086117-100T","price":300.0,"currency_code":"USD","in_stock":true},{"title":"500T","offer_id":42332766863435,"sku":"UA086117-500T","price":1000.0,"currency_code":"USD","in_stock":true},{"title":"2500T","offer_id":42332766896203,"sku":"UA086117-2500T","price":2955.0,"currency_code":"USD","in_stock":true},{"title":"10000T","offer_id":42332766928971,"sku":"UA086117-10000T","price":5425.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_44131323-3fc0-4cea-90dd-f8e25208389e.png?v=1788530501"},{"product_id":"unione-tr-fret-human-pcsk9d347yldlr-assay-kit-ua086124","title":"UniOne® TR FRET Human PCSK9(D347Y)\/LDLR Assay Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-80℃\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003eThe kit utilizes homogeneous time-resolved fluorescence (TR-FRET) technology to measure the interaction between Human PCSK9(D347Y) and Human LDLR. PCSK9(D347Y) is a mutant form of PCSK9 with higher affinity for LDLR. This method enables simple, rapid, and high-throughput screening of inhibitors and antibody blockers.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAs shown in the figure, the interaction between PCSK9(D347Y) and LDLR is detected using Eu-labeled anti-Tag1 antibody (TR-FRET donor) and Ac-labeled Tag2 antibody (TR-FRET acceptor). The binding of PCSK9(D347Y) to LDLR brings the donor and acceptor antibodies into proximity, allowing the excitation of the donor antibody to trigger fluorescence resonance energy transfer (FRET) to the acceptor antibody, resulting in a specific emission signal at 665 nm. Screened compounds, such as Pep 2-8, block the binding of PCSK9(D347Y) to LDLR, preventing FRET signal generation. The stronger the blocking effect of the screened compound, the lower the signal. The signal is proportional to the degree of interaction between PCSK9(D347Y) and LDLR. No washing steps are required.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"image_resized img img-fluid o_we_custom_image\" style=\"aspect-ratio:647\/582;width:55.21%;\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/product.template\/20998036\/f7f950f0-1148-438d-ab04-cef1e877d15f.png?access_token=fa60d4fb-f898-4480-bfc0-23866b7bd5de\" alt=\"\" width=\"647\" height=\"582\"\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003ctable border=\"1\" style=\"border-collapse:collapse;width:auto;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eComponent\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eConcentration\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e100T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e2500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e10000T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eStorage Temperature\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag1-LDLR protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003e0×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e400\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag2-PCSK9\u003c\/span\u003e\u003cspan\u003e(D347Y)\u003c\/span\u003e\u003cspan\u003e protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003e0×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAnti-Tag1 Eu antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003e0\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e250\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1000\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAnti-Tag2 Ac antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e12.5×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e40\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e200\u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4m\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003emL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e14\u003c\/span\u003e\u003cspan\u003emL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e60\u003c\/span\u003e\u003cspan\u003emL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e240\u003c\/span\u003e\u003cspan\u003emL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003eHere is the translated content in English, preserving the original formatting:```markdown\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003e1. Reagent Preparation\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003e1.1 Thaw all reagents to room temperature before use (allow at least 30 min for equilibration). The reaction system for 384-well plates is 20μL (reagent volumes are shown in the table below). Calculate the required volume before preparation and prepare accordingly. The following preparation is for reference only, using 500 reactions as an example.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003eTable 1. Reagent Preparation\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:1px solid #000000;width:auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"background-color:#8BAAD2;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eReagent Name\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003ePreparation\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eVolume per Well (μL)\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eSample Dilution\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan\u003eUse 1× Detection Buffer to dilute samples. If the sample stock is in DMSO, maintain consistent DMSO concentration in the system (≤2%).\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eTag1-LDLR Protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan\u003eDilute 20μL Tag1-LDLR stock with 1× Detection Buffer to 2mL, mix well.\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eTag2-PCSK9(D347Y) Protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\n\u003cspan\u003eDilute 20μL Tag2-PCSK9(D347Y) stock with 1× Detection Buffer to 2mL, \u003c\/span\u003e\u003cstrong\u003emix well.\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eAntibody Mix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\n\u003cspan\u003eDilute 50μL Anti-Tag1 Eu antibody with 2.45mL 1× Detection Buffer, \u003c\/span\u003e\u003cstrong\u003emix well;\u003c\/strong\u003e\u003cspan\u003e dilute 200μL Anti-Tag2 Ac antibody with 2.3mL 1× Detection Buffer, mix well. Combine Anti-Tag1 Eu and Anti-Tag2 Ac antibodies at 1:1 ratio to prepare Antibody Mix.\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003e1.2 Sample Gradient Dilution\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eUsing Pep 2-8 as an example, dilute with 1× Detection Buffer. To minimize matrix effects, use the same matrix as the sample. Adjust concentrations as needed.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003eTable 2. \u003c\/span\u003e\u003cspan\u003ePep 2-8 Preparation Table\u003c\/span\u003e\u003cspan style=\"color:#000000;\"\u003e (adjust as needed)\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:1px solid #000000;width:auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"background-color:#8BAAD2;text-align:center;vertical-align:middle;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003ePep 2-8 Prep Conc. (nM)\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eFinal Conc. (nM)\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003ePreparation Method\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"margin-right:8px;\"\u003e1\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e1000000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e100000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e12μL 1mM Pep 2-8\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"margin-right:8px;\"\u003e2\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e333333.33\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e33333.33\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e6μL ① +12μL 1× Detection Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"margin-right:8px;\"\u003e3\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e111111.11\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e11111.11\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e5μL ② +10μL 1× Detection Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"margin-right:8px;\"\u003e4\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e37037.04\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e3703.70\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e5μL ③ +10μL 1× Detection Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"margin-right:8px;\"\u003e5\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e12345.68\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e1234.57\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e5μL ④ +10μL 1× Detection Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"margin-right:8px;\"\u003e6\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e4115.23\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e411.52\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e5μL ⑤ +10μL 1× Detection Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"margin-right:8px;\"\u003e7\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e1371.74\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e137.17\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e5μL ⑥ +10μL 1× Detection Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"margin-right:8px;\"\u003e8\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e457.25\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e45.72\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e5μL ⑦ +10μL 1× Detection Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"margin-right:8px;\"\u003e9\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e152.42\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e15.24\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e5μL ⑧ +10μL 1× Detection Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eBlank\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL 1× Detection Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003e2. Sample Loading \u0026amp; Controls\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003e2.1 Test Samples: Add sequentially to 384-well plate—2μL gradient-diluted sample, 4μL Tag2-PCSK9(D347Y), 4μL Tag1-LDLR, 10μL Antibody Mix.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003e2.2 Blank Control (Blank): Replace sample with 2μL 1× Detection Buffer.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003e2.3 Eu Control (NC): 10μL 1× Detection Buffer + 10μL Antibody Mix.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003eAfter loading, centrifuge, seal, and incubate at RT for 2 hours.\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:1px solid #000000;width:auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eTest Sample\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eBlank Control\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eEu Control (NC)\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" rowspan=\"3\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eStep 1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e2μL gradient-diluted sample\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e2μL 1× Detection Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" rowspan=\"5\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL 1× Detection Buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e+10μL Antibody Mix\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"2\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e4μL Tag2-PCSK9(D347Y)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"2\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eSeal, centrifuge at 1000rpm for 1min,\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eincubate at RT for 10min\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" rowspan=\"3\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eStep 2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"2\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e4μL Tag1-LDLR\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"2\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL Antibody Mix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"3\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eSeal, centrifuge at 1000rpm for 1min, incubate at RT for 120min, read plate\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"margin-right:8px;\"\u003e3.\u003c\/span\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003eDetection\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003eMeasure using a TR-FRET-compatible microplate reader. Excitation: 320\/340nm; Emission: 620nm and 665nm.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#1655A5;\"\u003e\u003cstrong\u003e[Data Analysis]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1) Calculate Signal Ratio: (665nm\/620nm) ×10000.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan\u003eRatio = (665\/620) ×10000\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2) Calculate Net Signal:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan\u003eNet Signal = (Std-NC)\/NC×100\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3) Calculate CV (%):\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan\u003eCV (%) = Standard Deviation\/Mean Ratio × 100%\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003eRatio × 100%\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#1655A5;\"\u003e\u003cstrong\u003e[Example Data]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003eThe following data is illustrative and may vary based on instrumentation.\u003c\/span\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cimg class=\"image_resized\" style=\"aspect-ratio:672\/445;margin-left:30px;max-width:100%;vertical-align:middle;width:47.17%;\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/ae22ba3c-cf5c-4ae7-8d95-fef5b3386d69.png\" alt=\"\" width=\"672\" height=\"445\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003eNote: Recommended microplate (384-well, white, shallow well)\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e```\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"100T","offer_id":42332767649867,"sku":"UA086124-100T","price":730.0,"currency_code":"USD","in_stock":true},{"title":"500T","offer_id":42332767682635,"sku":"UA086124-500T","price":2180.0,"currency_code":"USD","in_stock":true},{"title":"2500T","offer_id":42332767715403,"sku":"UA086124-2500T","price":8175.0,"currency_code":"USD","in_stock":true},{"title":"10000T","offer_id":42332767748171,"sku":"UA086124-10000T","price":21800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_88a85b7c-5c0a-446f-9d5b-45e7077a2d2f.png?v=1788530520"},{"product_id":"unione-tr-fret-human-tslptslpr-binding-kit-ua086125","title":"Unione® TR-FRET Human TSLP\/TSLPR Binding Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e[\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003eBackground Introduction\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e]\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe kit employs homogeneous time-resolved fluorescence technology (TR-FRET) to measure\u003c\/span\u003e\u003cspan\u003eTSLP\u003c\/span\u003e\u003cspan\u003eand its interaction with\u003c\/span\u003e\u003cspan\u003eTSLPR\u003c\/span\u003e\u003cspan\u003e. This method enables simple and rapid high-throughput screening of inhibitors and antibody blockers.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/823af8fb-5d28-428f-86f3-08003668a835.png\" style=\"margin-left:30px; width:317px; height:auto; vertical-align:middle;\"\u003e\u003c\/p\u003e\n\u003cspan\u003eAs shown in the figure,\u003c\/span\u003e\u003cspan\u003ethe interaction between\u003c\/span\u003e\u003cspan\u003eTSLP\u003c\/span\u003e\u003cspan\u003eand\u003c\/span\u003e\u003cspan\u003eTSLPR\u003c\/span\u003e\u003cspan\u003eis detected using an Eu-labeled anti-Tag1 antibody (TR-FRET donor) and an Ac-labeled anti-Tag2 antibody (TR-FRET acceptor). Because\u003c\/span\u003e\u003cspan\u003eTSLP\u003c\/span\u003e\u003cspan\u003eand\u003c\/span\u003e\u003cspan\u003eTSLPRbind, bringing the donor antibody close to the acceptor antibody, excitation of the donor antibody triggers fluorescence resonance energy transfer (FRET) to the acceptor antibody, causing the acceptor antibody to emit a signal at a wavelength of 665 nm.\u003c\/span\u003e\u003cspan\u003eBosakitug\u003c\/span\u003e\u003cspan\u003eA positive drug can block\u003c\/span\u003e\u003cspan\u003eTSLP\u003c\/span\u003e\u003cspan\u003eand\u003c\/span\u003e\u003cspan\u003eTSLPR\u003c\/span\u003e\u003cspan\u003efrom binding, preventing the FRET signal from being produced; thus, the screened drug targets\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eTSLP\u003c\/span\u003e\u003cspan\u003eand\u003c\/span\u003e\u003cspan\u003eTSLPR\u003c\/span\u003e\u003cspan\u003eThe stronger the blocking effect, the lower the signal.\u003c\/span\u003e\u003cspan\u003eThis specific signal is related to\u003c\/span\u003e\u003cspan\u003eTSLP\u003c\/span\u003e\u003cspan\u003eand\u003c\/span\u003e\u003cspan\u003eTSLPR\u003c\/span\u003e\u003cspan\u003eis proportional to the degree of their interaction. This homogeneous assay is easy to perform and requires no washing steps.\u003c\/span\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e[\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003eProduct Components\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003eand Storage Conditions]\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse:collapse;width:auto;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eComponents\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eConcentration\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e100T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e2500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e10000T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eStorage Temperature\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag1-\u003c\/span\u003e\u003cspan\u003eTSLP\u003c\/span\u003e\u003cspan\u003eR\u003c\/span\u003e\u003cspan\u003eprotein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e400\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag2-\u003c\/span\u003e\u003cspan\u003eTSLP\u003c\/span\u003e\u003cspan\u003eprotein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e400\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAnti-Tag1 Eu antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e25\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e125\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003e0\u003c\/span\u003e\u003cspan\u003e0μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAnti-Tag2 Ac antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e25\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e0\u003c\/span\u003e\u003cspan\u003e0μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003e0\u003c\/span\u003e\u003cspan\u003e0μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e0\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e400\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003emL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003emL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e40\u003c\/span\u003e\u003cspan\u003emL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan\u003eCaution: After the first thaw, immediately aliquot and store at the recommended temperature. Avoid storing diluted samples or subjecting them to repeated freeze-thaw cycles.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e[Experimental Procedure and Operations]\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e,\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eReagent Preparation\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e.1\u003c\/span\u003e\u003cspan\u003eBefore use, thaw all reagents at room temperature (allow them to equilibrate at room temperature for at least 30 minutes). The reaction system in a 384-well plate is 20\u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan\u003eL (the amount of reagents for the reaction system is shown in the table). Calculate the required volume before preparation and prepare accordingly; the following preparation is for reference only, using 500 reactions as an example.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eTable 1\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003cspan\u003eReagent Preparation\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eReagent Name\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eConfiguration\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eAmount per Detection Well (μL)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDetection\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003ebuffer\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eTake 2\u003c\/span\u003e\u003cspan\u003emL of 10\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eDetection\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eAdd 18 mL of deionized water to the buffer, dilute\u003c\/span\u003e\u003cspan\u003eto\u003c\/span\u003e\u003cspan\u003e1×, mix\u003c\/span\u003e\u003cspan\u003ethoroughly and set aside\u003c\/span\u003e\u003cspan\u003efor use\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003cspan\u003e-\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag1-\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTSLP\u003c\/span\u003e\u003cspan\u003eR\u003c\/span\u003e\u003cspan\u003eprotein\u003c\/span\u003e\u003cspan\u003eTake 2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003e0\u003c\/span\u003e\u003cspan\u003eμL of Tag1-\u003c\/span\u003e\u003cspan\u003eTSLP\u003c\/span\u003e\u003cspan\u003eR\u003c\/span\u003e\u003cspan\u003eprotein stock solution\u003c\/span\u003e\u003cspan\u003e,\u003c\/span\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003cspan\u003ediluted with Detection buffer\u003c\/span\u003e\u003cspan\u003eto\u003c\/span\u003e\u003cspan\u003e2mL ,\u003c\/span\u003e\u003cspan\u003emix well and set aside\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003efor use\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag2-\u003c\/span\u003e\u003cspan\u003eTSLP\u003c\/span\u003e\u003cspan\u003eprotein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003etake 2\u003c\/span\u003e\u003cspan\u003e0\u003c\/span\u003e\u003cspan\u003eμL of Tag2-\u003c\/span\u003e\u003cspan\u003eTSLP\u003c\/span\u003e\u003cspan\u003eprotein stock solution, diluted 1\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003cspan\u003ewith Detection buffer\u003c\/span\u003e\u003cspan\u003eto\u003c\/span\u003e\u003cspan\u003e2mL ,\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eMix well and set aside\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eMix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eTake\u003c\/span\u003e\u003cspan\u003e25\u003c\/span\u003e\u003cspan\u003eμL of Anti-Tag1 Eu antibody stock solution, add 1\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003cspan\u003eDetection buffer 2.4\u003c\/span\u003e\u003cspan\u003e75\u003c\/span\u003e\u003cspan\u003emL,\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003emix well and set aside\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003etake\u003c\/span\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e00 μL of Anti-Tag2\u003c\/span\u003e\u003cspan\u003eAc\u003c\/span\u003e\u003cspan\u003eantibody stock solution, add 1\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003cspan\u003eDetection buffer 2.\u003c\/span\u003e\u003cspan\u003e4\u003c\/span\u003e\u003cspan\u003emL,\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003emix well and set aside\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eAnti-Tag1 Eu antibody and Anti-Tag2\u003c\/span\u003e\u003cspan\u003eAc\u003c\/span\u003e\u003cspan\u003eantibody are each diluted and then mixed 1:1\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003emix well and set aside\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan\u003e1.2\u003c\/span\u003e\u003cspan\u003esample to be tested\u003c\/span\u003e\u003cspan\u003egradient dilution\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003etaking\u003c\/span\u003e\u003cspan\u003eBosakitug\u003c\/span\u003e\u003cspan\u003eas an example, the diluent is 1× Detection buffer,\u003c\/span\u003e\u003cspan\u003eto reduce the influence of matrix effect interference, it is recommended to use a solution with the same matrix as the sample to be tested for dilution; and\u003c\/span\u003e\u003cspan\u003ethe sample to be tested should be adjusted according to its actual concentration.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eTable\u003c\/span\u003e\u003cspan\u003e2.\u003c\/span\u003e\u003cspan\u003ePositive drug\u003c\/span\u003e\u003cspan\u003eBosakitug\u003c\/span\u003e\u003cspan\u003eGradient dilution\u003c\/span\u003e\u003cspan\u003e(adjusted according to the actual situation)\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eBosakitug\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eFinal concentration\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003enM\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eBosakitug\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eConfiguration concentration\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003enM\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003ePreparation method\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e①\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-style:italic;text-decoration:underline;color:#000000\"\u003e10.00\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-style:italic;text-decoration:underline;color:#000000\"\u003e100.00\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan\u003eM\u003c\/span\u003e\u003cspan\u003eBosakitug\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e57\u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e②\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-style:italic;text-decoration:underline;color:#000000\"\u003e5.00\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-style:italic;text-decoration:underline;color:#000000\"\u003e50.00\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e30\u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e①\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e30\u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e③\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-style:italic;text-decoration:underline;color:#000000\"\u003e2.50\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-style:italic;text-decoration:underline;color:#000000\"\u003e25.00\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e30\u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e②\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e30\u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e④\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-style:italic;text-decoration:underline;color:#000000\"\u003e1.25\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-style:italic;text-decoration:underline;color:#000000\"\u003e12.50\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e30\u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e③\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e30\u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑤\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-style:italic;text-decoration:underline;color:#000000\"\u003e0.63\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-style:italic;text-decoration:underline;color:#000000\"\u003e6.25\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e30\u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e④\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e30\u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑥\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-style:italic;text-decoration:underline;color:#000000\"\u003e0.31\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-style:italic;text-decoration:underline;color:#000000\"\u003e3.13\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e30\u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e⑤\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e30\u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑦\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-style:italic;text-decoration:underline;color:#000000\"\u003e0.16\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-style:italic;text-decoration:underline;color:#000000\"\u003e1.56\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e30\u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e⑥\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e30\u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑧\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-style:italic;text-decoration:underline;color:#000000\"\u003e0.08\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-style:italic;text-decoration:underline;color:#000000\"\u003e0.78\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e30\u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e⑦\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e30\u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑨\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-style:italic;text-decoration:underline;color:#000000\"\u003e0.04\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-style:italic;text-decoration:underline;color:#000000\"\u003e0.39\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e30\u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e⑧\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e30\u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eBlank\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e30\u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003e2\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e,\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eSample addition and controls\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.1 Sample addition order for experimental wells: 2 μL of the sample to be tested, 4 μL\u003c\/span\u003e\u003cspan\u003eTag\u003c\/span\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e-\u003c\/span\u003e\u003cspan\u003eTSLP\u003c\/span\u003e\u003cspan\u003eprotein\u003c\/span\u003e\u003cspan\u003eworking solution, 4 μL\u003c\/span\u003e\u003cspan\u003eTag\u003c\/span\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e-\u003c\/span\u003e\u003cspan\u003eTSLPR\u003c\/span\u003e\u003cspan\u003eprotein\u003c\/span\u003e\u003cspan\u003eworking solution, a mixed solution of 10 μL, added sequentially into the 384-well plate\u003c\/span\u003e\u003cspan\u003e;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.2\u003c\/span\u003e\u003cspan\u003emaximum value\u003c\/span\u003e\u003cspan\u003econtrol\u003c\/span\u003e\u003cspan\u003e: 2 μL of 1× Detection buffer diluted\u003c\/span\u003e\u003cspan\u003eliquid,\u003c\/span\u003e\u003cspan\u003e4 μL\u003c\/span\u003e\u003cspan\u003eTag\u003c\/span\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e-\u003c\/span\u003e\u003cspan\u003eTSLP\u003c\/span\u003e\u003cspan\u003eprotein\u003c\/span\u003e\u003cspan\u003eworking solution, 4 μL\u003c\/span\u003e\u003cspan\u003eTag\u003c\/span\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e-\u003c\/span\u003e\u003cspan\u003eTSLPR\u003c\/span\u003e\u003cspan\u003eprotein\u003c\/span\u003e\u003cspan\u003eworking solution, a mixed solution of 10 μL\u003c\/span\u003e\u003cspan\u003e,\u003c\/span\u003e\u003cspan\u003eadded sequentially into the 384-well plate\u003c\/span\u003e\u003cspan\u003e;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.3 Quality control well (\u003c\/span\u003e\u003cspan\u003eNC\u003c\/span\u003e\u003cspan\u003e):\u003c\/span\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003eμL of 1× Detection buffer plus\u003c\/span\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003cspan\u003eMix\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAfter all samples have been added, centrifuge, cover with a plate seal, and incubate at room temperature\u003c\/span\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003ehour.\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse:collapse;width:auto;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eMaximum value\u003c\/span\u003e\u003cspan\u003eControl\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eSample\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eNC\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eStep one\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2 μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1× Detection\u003c\/span\u003e\u003cspan\u003eB\u003c\/span\u003e\u003cspan\u003euffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eGradient dilution of the sample to be tested\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"4\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e0\u003c\/span\u003e\u003cspan\u003euL 1× Detection\u003c\/span\u003e\u003cspan\u003eB\u003c\/span\u003e\u003cspan\u003euffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"2\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4 μL\u003c\/span\u003e\u003cspan\u003eTag\u003c\/span\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e-\u003c\/span\u003e\u003cspan\u003eTSLP\u003c\/span\u003e\u003cspan\u003eprotein\u003c\/span\u003e\u003cspan\u003eworking solution\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"2\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eincubate for 10 min\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"2\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eStep two\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"2\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4 μL\u003c\/span\u003e\u003cspan\u003eTag\u003c\/span\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e-\u003c\/span\u003e\u003cspan\u003eTSLPR\u003c\/span\u003e\u003cspan\u003eprotein\u003c\/span\u003e\u003cspan\u003eworking solution\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003cspan\u003eMix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eStep three\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eseal the plate with a membrane, incubate at room temperature\u003c\/span\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003eh\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e3,\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003edetection\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eDetect on an ELISA reader compatible with TR-FRET\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003cspan\u003eExcitation light is 320\/340 nm, detecting emission wavelengths at 620 nm and 665 nm\u003c\/span\u003e\u003cspan\u003eemission\u003c\/span\u003e\u003cspan\u003ewavelengths.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e[Result Calculation]\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1)\u003c\/span\u003e\u003cspan\u003eCalculate the signal value\u003c\/span\u003e\u003cspan\u003e(Ratio)\u003c\/span\u003e\u003cspan\u003e: The fluorescence signal at 665 nm divided by the fluorescence signal at 620 nm, then multiplied by 10,000\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eRatio\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e=\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e(665\/620)\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003cspan\u003e10000\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2)\u003c\/span\u003e\u003cspan\u003eCalculated based on the signal value\u003c\/span\u003e\u003cspan\u003eNet signal\u003c\/span\u003e\u003cspan\u003e:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eNet signal\u003c\/span\u003e\u003cspan\u003e= (Std-\u003c\/span\u003e\u003cspan\u003eNC\u003c\/span\u003e\u003cspan\u003e)\/\u003c\/span\u003e\u003cspan\u003eNC\u003c\/span\u003e\u003cspan\u003e×100\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3)\u003c\/span\u003e\u003cspan\u003eCalculate CV (%)\u003c\/span\u003e\u003cspan\u003e:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eCV (%) = Standard Deviation\/Mean Ratio × 100%\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e[Data Example]\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe following data cannot replace the data obtained in experiments; it is only for illustration, and the results may\u003c\/span\u003e\u003cspan\u003edepend on the plate reader\u003c\/span\u003e\u003cspan\u003einstrument\u003c\/span\u003e\u003cspan\u003evaries.\u003c\/span\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/f427108f-edbc-4213-a6ce-b52fcecd7d36.png\" style=\"margin-left:30px; width:319px; height:auto; vertical-align:middle;\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003eNote: Recommended\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003emicroplate (384-well plate, white, shallow wells)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"100T","offer_id":42332770041931,"sku":"UA086125-100T","price":730.0,"currency_code":"USD","in_stock":true},{"title":"500T","offer_id":42332770074699,"sku":"UA086125-500T","price":2180.0,"currency_code":"USD","in_stock":true},{"title":"2500T","offer_id":42332770107467,"sku":"UA086125-2500T","price":8175.0,"currency_code":"USD","in_stock":true},{"title":"10000T","offer_id":42332770140235,"sku":"UA086125-10000T","price":21800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_e0b656a9-3c0e-4ebe-a93c-ce6e291e948d.png?v=1790650806"},{"product_id":"unione-tr-fret-human-cgmp-detection-kit-ua086127","title":"UniOne® TR-FRET Human cGMP Detection Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-80℃\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003eThe kit employs homogeneous time-resolved fluorescence technology (TR-FRET)\u003c\/span\u003e\u003cspan\u003e,\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eenabling precise measurement of cellular cGMP production.\u003c\/span\u003e\u003cspan\u003e This method provides a high-throughput format for simple and rapid screening of molecules that modulate phosphodiesterase activity.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAs illustrated, biotin-labeled cGMP can bind to streptavidin (SA), while the Eu-labeled anti-cGMP antibody (TR-FRET donor) and the Ac-labeled anti-Tag2 antibody (TR-FRET acceptor) bind to cGMP and biotin, respectively. The proximity of the donor and acceptor antibodies allows the excitation of the donor antibody to trigger fluorescence resonance energy transfer (FRET) to the acceptor antibody, resulting in a specific 665 nm emission signal. When cGMP is present in cell samples or standard curves, it competes with biotin-labeled cGMP for binding to the Eu-labeled anti-cGMP antibody, thereby reducing the FRET signal. This specific signal is proportional to the degree of interaction between the Eu-labeled anti-cGMP antibody, biotin-labeled cGMP, and \u003c\/span\u003e\u003cspan\u003eSA\u003c\/span\u003e\u003cspan\u003e. This homogeneous assay is simple to perform and requires no washing steps.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/999c3963-fd43-4082-b051-065fb068a173.png\" style=\"margin-left:30px; width:622px; height:auto; vertical-align:middle;\" alt=\"\"\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:none;margin-left:6.7500pt;margin-right:6.7500pt;width:510.7500pt;\" border=\"0\" cellspacing=\"0\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height:29.1500pt;\"\u003e\n\u003ctd style=\"background-color:rgb(139,170,210);border-bottom:1.0000pt solid windowtext;border-left:1.0000pt solid windowtext;border-right:1.0000pt solid windowtext;border-top:1.0000pt solid windowtext;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;width:144.4000pt;\" width=\"288\" valign=\"center\"\u003e\u003cp style=\"text-align:center;text-autospace:ideograph-numeric;\"\u003e\u003cspan style=\"color:rgb(0,0,0);\"\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/span\u003e\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:rgb(139,170,210);border-bottom:1.0000pt solid windowtext;border-left:none;border-right:1.0000pt solid windowtext;border-top:1.0000pt solid windowtext;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;width:59.9000pt;\" width=\"119\" valign=\"center\"\u003e\u003cp style=\"text-align:center;text-autospace:ideograph-numeric;\"\u003e\u003cspan style=\"color:rgb(0,0,0);\"\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/span\u003e\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:rgb(139,170,210);border-bottom:1.0000pt solid windowtext;border-left:none;border-right:1.0000pt solid windowtext;border-top:1.0000pt solid windowtext;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;width:58.7500pt;\" width=\"117\" valign=\"center\"\u003e\u003cp style=\"text-align:center;text-autospace:ideograph-numeric;\"\u003e\u003cspan style=\"color:rgb(0,0,0);\"\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e\u003cstrong\u003e100T\u003c\/strong\u003e\u003c\/span\u003e\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:rgb(139,170,210);border-bottom:1.0000pt solid windowtext;border-left:none;border-right:1.0000pt solid windowtext;border-top:1.0000pt solid windowtext;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;width:58.7500pt;\" width=\"117\" valign=\"center\"\u003e\u003cp style=\"text-align:center;text-autospace:ideograph-numeric;\"\u003e\u003cspan style=\"color:rgb(0,0,0);\"\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e\u003cstrong\u003e500T\u003c\/strong\u003e\u003c\/span\u003e\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:rgb(139,170,210);border-bottom:1.0000pt solid windowtext;border-left:none;border-right:1.0000pt solid windowtext;border-top:1.0000pt solid windowtext;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;width:58.7500pt;\" width=\"117\" valign=\"center\"\u003e\u003cp style=\"text-align:center;text-autospace:ideograph-numeric;\"\u003e\u003cspan style=\"color:rgb(0,0,0);\"\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e\u003cstrong\u003e2500T\u003c\/strong\u003e\u003c\/span\u003e\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:rgb(139,170,210);border-bottom:1.0000pt solid windowtext;border-left:none;border-right:1.0000pt solid windowtext;border-top:1.0000pt solid windowtext;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;width:58.7500pt;\" width=\"117\" valign=\"center\"\u003e\u003cp style=\"text-align:center;text-autospace:ideograph-numeric;\"\u003e\u003cspan style=\"color:rgb(0,0,0);\"\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e\u003cstrong\u003e10000T\u003c\/strong\u003e\u003c\/span\u003e\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:rgb(139,170,210);border-bottom:1.0000pt solid windowtext;border-left:none;border-right:1.0000pt solid windowtext;border-top:1.0000pt solid windowtext;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;width:71.4500pt;\" width=\"142\" valign=\"center\"\u003e\u003cp style=\"text-align:center;text-autospace:ideograph-numeric;\"\u003e\u003cspan style=\"color:rgb(0,0,0);\"\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e\u003cstrong\u003eStorage Temperature\u003c\/strong\u003e\u003c\/span\u003e\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:29.1500pt;\"\u003e\n\u003ctd style=\"border-bottom:1.0000pt solid windowtext;border-left:1.0000pt solid windowtext;border-right:1.0000pt solid windowtext;border-top:none;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;width:144.4000pt;\" width=\"288\" valign=\"center\"\u003e\u003cp style=\"line-height:114%;text-align:center;\"\u003e\u003cspan style=\"color:rgb(0,0,0);\"\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003eBiotin\u003c\/span\u003e\u003c\/font\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e-cGMP protein\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:1.0000pt solid windowtext;border-left:none;border-right:1.0000pt solid windowtext;border-top:none;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;width:59.9000pt;\" width=\"119\" valign=\"center\"\u003e\u003cp style=\"line-height:114%;text-align:center;\"\u003e\u003cspan style=\"color:rgb(0,0,0);\"\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e100×\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:1.0000pt solid windowtext;border-left:none;border-right:1.0000pt solid windowtext;border-top:none;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;width:58.7500pt;\" width=\"117\" valign=\"center\"\u003e\u003cp style=\"line-height:114%;text-align:center;\"\u003e\u003cspan style=\"color:rgb(0,0,0);\"\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e5\u003c\/span\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003eμ\u003c\/span\u003e\u003c\/font\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003eL\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:1.0000pt solid 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5.4000pt;width:71.4500pt;\" width=\"142\" valign=\"center\"\u003e\u003cp style=\"line-height:114%;text-align:center;\"\u003e\u003cspan style=\"color:rgb(0,0,0);\"\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e-80℃\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:29.1500pt;\"\u003e\n\u003ctd style=\"border-bottom:1.0000pt solid windowtext;border-left:1.0000pt solid windowtext;border-right:1.0000pt solid windowtext;border-top:none;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;width:144.4000pt;\" width=\"288\" valign=\"center\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:rgb(0,0,0);\"\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003ecGMP \u003c\/span\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:10.0000pt;\"\u003ecalibrator\u003c\/span\u003e\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd 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style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003eμ\u003c\/span\u003e\u003c\/font\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003eL\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:1.0000pt solid windowtext;border-left:none;border-right:1.0000pt solid windowtext;border-top:none;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;width:58.7500pt;\" width=\"117\" valign=\"center\"\u003e\u003cp style=\"line-height:114%;text-align:center;\"\u003e\u003cspan style=\"color:rgb(0,0,0);\"\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e50\u003c\/span\u003e\u003c\/font\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e0\u003c\/span\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003eμ\u003c\/span\u003e\u003c\/font\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003eL\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:1.0000pt solid windowtext;border-left:none;border-right:1.0000pt solid windowtext;border-top:none;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;width:58.7500pt;\" width=\"117\" valign=\"center\"\u003e\u003cp style=\"line-height:114%;text-align:center;\"\u003e\u003cspan style=\"color:rgb(0,0,0);\"\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e2\u003c\/span\u003e\u003c\/font\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003emL\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:1.0000pt solid windowtext;border-left:none;border-right:1.0000pt solid windowtext;border-top:none;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;width:71.4500pt;\" width=\"142\" valign=\"center\"\u003e\u003cp style=\"line-height:114%;text-align:center;\"\u003e\u003cspan style=\"color:rgb(0,0,0);\"\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e-80℃\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:29.1500pt;\"\u003e\n\u003ctd style=\"border-bottom:1.0000pt solid windowtext;border-left:1.0000pt solid windowtext;border-right:1.0000pt solid windowtext;border-top:none;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;width:144.4000pt;\" width=\"288\" valign=\"center\"\u003e\u003cp style=\"line-height:114%;text-align:center;\"\u003e\u003cspan style=\"color:rgb(0,0,0);\"\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003eLysis \u0026amp; Detect Buffer\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:1.0000pt solid windowtext;border-left:none;border-right:1.0000pt solid windowtext;border-top:none;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;width:59.9000pt;\" width=\"119\" valign=\"center\"\u003e\u003cp style=\"line-height:114%;text-align:center;\"\u003e\u003cspan style=\"color:rgb(0,0,0);\"\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e1\u003c\/span\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e×\u003c\/span\u003e\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:1.0000pt solid windowtext;border-left:none;border-right:1.0000pt solid windowtext;border-top:none;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;width:58.7500pt;\" width=\"117\" valign=\"center\"\u003e\u003cp style=\"line-height:114%;text-align:center;\"\u003e\u003cspan style=\"color:rgb(0,0,0);\"\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e2m\u003c\/span\u003e\u003c\/font\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003eL\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:1.0000pt solid windowtext;border-left:none;border-right:1.0000pt solid windowtext;border-top:none;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;width:58.7500pt;\" width=\"117\" valign=\"center\"\u003e\u003cp style=\"line-height:114%;text-align:center;\"\u003e\u003cspan style=\"color:rgb(0,0,0);\"\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e10\u003c\/span\u003e\u003c\/font\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003emL\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:1.0000pt solid windowtext;border-left:none;border-right:1.0000pt solid windowtext;border-top:none;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;width:58.7500pt;\" width=\"117\" valign=\"center\"\u003e\u003cp style=\"line-height:114%;text-align:center;\"\u003e\u003cspan style=\"color:rgb(0,0,0);\"\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e5\u003c\/span\u003e\u003c\/font\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e0mL\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:1.0000pt solid windowtext;border-left:none;border-right:1.0000pt solid windowtext;border-top:none;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;width:58.7500pt;\" width=\"117\" valign=\"center\"\u003e\u003cp style=\"line-height:114%;text-align:center;\"\u003e\u003cspan style=\"color:rgb(0,0,0);\"\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e20\u003c\/span\u003e\u003c\/font\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e0mL\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:1.0000pt solid windowtext;border-left:none;border-right:1.0000pt solid windowtext;border-top:none;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;width:71.4500pt;\" width=\"142\" valign=\"center\"\u003e\u003cp style=\"line-height:114%;text-align:center;\"\u003e\u003cspan style=\"color:rgb(0,0,0);\"\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e-80℃\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:29.6500pt;\"\u003e\n\u003ctd style=\"border-bottom:1.0000pt solid windowtext;border-left:1.0000pt solid windowtext;border-right:1.0000pt solid windowtext;border-top:none;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;width:144.4000pt;\" width=\"288\" valign=\"center\"\u003e\u003cp style=\"line-height:114%;text-align:center;\"\u003e\u003cspan style=\"color:rgb(0,0,0);\"\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003eStimulation Buffer (without IBMX)\u003c\/span\u003e\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:1.0000pt solid windowtext;border-left:none;border-right:1.0000pt solid windowtext;border-top:none;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;width:59.9000pt;\" width=\"119\" valign=\"center\"\u003e\u003cp style=\"line-height:114%;text-align:center;\"\u003e\u003cspan style=\"color:rgb(0,0,0);\"\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e1\u003c\/span\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e×\u003c\/span\u003e\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:1.0000pt solid windowtext;border-left:none;border-right:1.0000pt solid windowtext;border-top:none;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;width:58.7500pt;\" width=\"117\" valign=\"center\"\u003e\u003cp style=\"line-height:114%;text-align:center;\"\u003e\u003cspan style=\"color:rgb(0,0,0);\"\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e2m\u003c\/span\u003e\u003c\/font\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003eL\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:1.0000pt solid windowtext;border-left:none;border-right:1.0000pt solid windowtext;border-top:none;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;width:58.7500pt;\" width=\"117\" valign=\"center\"\u003e\u003cp style=\"line-height:114%;text-align:center;\"\u003e\u003cspan style=\"color:rgb(0,0,0);\"\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e10\u003c\/span\u003e\u003c\/font\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003emL\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:1.0000pt solid windowtext;border-left:none;border-right:1.0000pt solid windowtext;border-top:none;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;width:58.7500pt;\" width=\"117\" valign=\"center\"\u003e\u003cp style=\"line-height:114%;text-align:center;\"\u003e\u003cspan style=\"color:rgb(0,0,0);\"\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e5\u003c\/span\u003e\u003c\/font\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e0mL\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:1.0000pt solid windowtext;border-left:none;border-right:1.0000pt solid windowtext;border-top:none;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;width:58.7500pt;\" width=\"117\" valign=\"center\"\u003e\u003cp style=\"line-height:114%;text-align:center;\"\u003e\u003cspan style=\"color:rgb(0,0,0);\"\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e20\u003c\/span\u003e\u003c\/font\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e0mL\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:1.0000pt solid windowtext;border-left:none;border-right:1.0000pt solid windowtext;border-top:none;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;width:71.4500pt;\" width=\"142\" valign=\"center\"\u003e\u003cp style=\"line-height:114%;text-align:center;\"\u003e\u003cspan style=\"color:rgb(0,0,0);\"\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e-80℃\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003eHere is the English translation of the provided content while preserving the exact HTML structure and formatting: ```html\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e1. Reagent Preparation\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1.1 Thaw all reagents to room temperature before use. The reaction system for the 384-well plate is 20μL (reagent volumes are shown in the table below). Calculate the required volume before preparation. The following preparation is for reference only, using 500 reactions as an example.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan\u003eTable 1. Reagent Preparation and Volumes\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:none;width:505.0000pt;\" border=\"1\" cellspacing=\"0\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height:30.0000pt;\"\u003e\n\u003ctd style=\"background-color:rgb(139,170,210);border-bottom:1.0000pt solid windowtext;border-left:1.0000pt solid windowtext;border-right:1.0000pt solid windowtext;border-top:1.0000pt solid windowtext;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;width:119.0000pt;\" width=\"238\" valign=\"center\"\u003e\u003cp style=\"text-align:center;text-autospace:ideograph-numeric;\"\u003e\u003cspan style=\"color:rgb(0,0,0);\"\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e\u003cstrong\u003eReagent Name\u003c\/strong\u003e\u003c\/span\u003e\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:rgb(139,170,210);border-bottom:1.0000pt solid windowtext;border-left:1.0000pt solid windowtext;border-right:1.0000pt solid windowtext;border-top:1.0000pt solid windowtext;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;width:300.3500pt;\" width=\"600\" valign=\"center\"\u003e\u003cp style=\"text-align:center;text-autospace:ideograph-numeric;\"\u003e\u003cspan style=\"color:rgb(0,0,0);\"\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e\u003cstrong\u003ePreparation\u003c\/strong\u003e\u003c\/span\u003e\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:rgb(139,170,210);border-bottom:1.0000pt solid windowtext;border-left:1.0000pt solid windowtext;border-right:1.0000pt solid windowtext;border-top:1.0000pt solid windowtext;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;width:85.6500pt;\" width=\"171\" valign=\"center\"\u003e\u003cp style=\"text-align:center;text-autospace:ideograph-numeric;\"\u003e\u003cspan style=\"color:rgb(0,0,0);\"\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e\u003cstrong\u003eVolume per Well (μL)\u003c\/strong\u003e\u003c\/span\u003e\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:30.0000pt;\"\u003e\n\u003ctd style=\"border-bottom:1.0000pt solid windowtext;border-left:1.0000pt solid windowtext;border-right:1.0000pt solid windowtext;border-top:none;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;width:119.0000pt;\" width=\"238\" valign=\"center\"\u003e\u003cp style=\"line-height:114%;text-align:center;\"\u003e\u003cspan style=\"color:rgb(0,0,0);\"\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003eAnti-\u003c\/span\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003ecGMP\u003c\/span\u003e\u003c\/font\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e Eu antibody\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:1.0000pt solid windowtext;border-left:1.0000pt solid windowtext;border-right:1.0000pt solid windowtext;border-top:none;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;width:300.3500pt;\" width=\"600\" valign=\"top\"\u003e\u003cp style=\"line-height:114%;text-align:justify;text-justify:inter-ideograph;\"\u003e\u003cspan style=\"color:rgb(0,0,0);\"\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003eTake 25μL of \u003c\/span\u003e\u003c\/font\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003eAnti-cGMP Eu antibody\u003c\/span\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e stock solution and dilute to 2.5mL with \u003c\/span\u003e\u003c\/font\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003eLysis \u0026amp; Detect Buffer\u003c\/span\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e, mix well.\u003c\/span\u003e\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:1.0000pt solid windowtext;border-left:1.0000pt solid windowtext;border-right:1.0000pt solid windowtext;border-top:none;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;width:85.6500pt;\" width=\"171\" valign=\"center\"\u003e\u003cp style=\"line-height:114%;text-align:center;\"\u003e\u003cspan style=\"color:rgb(0,0,0);\"\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e5μL\u003c\/span\u003e\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:2.7000pt;\"\u003e\n\u003ctd style=\"border-bottom:1.0000pt solid windowtext;border-left:1.0000pt solid windowtext;border-right:1.0000pt solid windowtext;border-top:none;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;width:119.0000pt;\" width=\"238\" valign=\"center\"\u003e\u003cp style=\"line-height:114%;text-align:center;\"\u003e\u003cspan style=\"color:rgb(0,0,0);\"\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003ecGMP Ac Mix\u003c\/span\u003e\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:1.0000pt solid windowtext;border-left:1.0000pt solid windowtext;border-right:1.0000pt solid windowtext;border-top:none;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;width:300.3500pt;\" width=\"600\" valign=\"top\"\u003e\u003cp style=\"line-height:114%;text-align:justify;text-justify:inter-ideograph;\"\u003e\u003cspan style=\"color:rgb(0,0,0);\"\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003eTake 12.5μL of Biotin\u003c\/span\u003e\u003c\/font\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e-\u003c\/span\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003ecGMP protein stock solution and dilute to 1.25mL with \u003c\/span\u003e\u003c\/font\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003eLysis \u0026amp; Detect Buffer\u003c\/span\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e, mix well; take 100μL of \u003c\/span\u003e\u003c\/font\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003eSA-Ac\u003c\/span\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e stock solution and add to 1.25mL of \u003c\/span\u003e\u003c\/font\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003eLysis \u0026amp; Detect Buffer\u003c\/span\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e, mix well. Mix the diluted Biotin\u003c\/span\u003e\u003c\/font\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e-\u003c\/span\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003ecGMP protein and \u003c\/span\u003e\u003c\/font\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003eSA-Ac\u003c\/span\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e in a 1:1 ratio to prepare the cGMP Ac Mix.\u003c\/span\u003e\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:1.0000pt solid windowtext;border-left:1.0000pt solid windowtext;border-right:1.0000pt solid windowtext;border-top:none;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;width:85.6500pt;\" width=\"171\" valign=\"center\"\u003e\u003cp style=\"line-height:114%;text-align:center;\"\u003e\u003cspan style=\"color:rgb(0,0,0);\"\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e5μL\u003c\/span\u003e\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1.2 Gradient Dilution of Test Samples\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003ePrepare according to experimental requirements. The following is for reference only.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eUsing cGMP calibrator as an example, the dilution buffer is Stimulation Buffer. Adjust the sample concentration as needed.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan\u003eTable 2. cGMP Calibrator Preparation\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:1px solid #000000;width:auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eFinal Concentration (nM)\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003ePreparation Concentration (nM)\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003ePreparation Method\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"margin-right:8px;\"\u003e1\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e1,250.000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e5,000.000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e1μL of 500μM stock + 99μL Stimulation Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"margin-right:8px;\"\u003e2\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e416.667\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e1,666.667\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e20μL of ① + 40μL Stimulation Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"margin-right:8px;\"\u003e3\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e138.889\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e555.556\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e20μL of ② + 40μL Stimulation Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"margin-right:8px;\"\u003e4\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e46.296\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e185.185\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e20μL of ③ + 40μL Stimulation Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"margin-right:8px;\"\u003e5\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e15.432\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e61.728\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e20μL of ④ + 40μL Stimulation Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"margin-right:8px;\"\u003e6\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e5.144\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e20.576\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e20μL of ⑤ + 40μL Stimulation Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"margin-right:8px;\"\u003e7\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e1.715\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e6.859\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e20μL of ⑥ + 40μL Stimulation Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"margin-right:8px;\"\u003e8\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e0.572\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e2.286\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e20μL of ⑦ + 40μL Stimulation Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"margin-right:8px;\"\u003e9\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e0.191\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e0.762\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e20μL of ⑧ + 40μL Stimulation Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"margin-right:8px;\"\u003e10\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e0.064\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e0.254\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e20μL of ⑨ + 40μL Stimulation Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"margin-right:8px;\"\u003e11\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e0.021\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e0.085\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e20μL of ⑩ + 40μL Stimulation Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eBlank\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e40μL of 1× Stimulation Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1.3 Pre-Treatment for Cell-Based Experiments\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e① Stimulation Buffer is used to dilute cells and stimulant drugs. The Stimulation Buffer provided in the kit does not contain IBMX. If the experiment aims to quantify the final concentration of cGMP in cells, it is necessary to add an appropriate concentration of IBMX to prevent cGMP degradation. If the experiment aims to evaluate the performance of PDE inhibitors, IBMX should not be added.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e② Before conducting formal experiments, determine the number of cells per well and the incubation time with drugs based on cell and drug characteristics to ensure that the cGMP levels produced by cells fall within the linear range of the calibration curve.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2. Sample Addition and Controls\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.1 cGMP Standard Curve: 5μL of gradient-diluted cGMP reference, 5μL Stimulation Buffer, 5μL Anti-cGMP Eu antibody working solution, incubate for 10min, then add 5μL cGMP Ac Mix.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.2 Test Samples: 5μL of gradient-diluted test sample, 5μL Stimulation Buffer, 5μL Anti-cGMP Eu antibody working solution, incubate for 10min, then add 5μL cGMP Ac Mix.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.3 Cell-Based Experiments: Based on pre-experiment results, add 5μL of cells, 5μL of gradient-diluted drug, and incubate for the determined time. After incubation, add 5μL Anti-cGMP Eu antibody working solution, incubate at room temperature for 10min, then add 5μL cGMP Ac Mix.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.4 Negative Control (NC): 5μL Stimulation Buffer instead of test sample or 5μL of untreated cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:1px solid #000000;width:auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003ecGMP Standard Curve\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eTest Samples\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eCell Experiments\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eNegative Control (NC)\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" rowspan=\"4\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eStep 1\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e5μL cGMP calibrator\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e5μL test sample\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e5μL cells\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e5μL Stimulation Buffer or 5μL cells\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e5μL Stimulation Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e5μL Stimulation Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e5μL drug※\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e5μL Stimulation Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"4\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e5μL Anti-cGMP Eu antibody working solution\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"4\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eIncubate at room temperature for 10min\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" rowspan=\"2\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eStep 2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"4\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e5μL cGMP Ac Mix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"4\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eSeal the plate, centrifuge at 1000rpm for 1min to mix, incubate at room temperature for 120min, then read the plate.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"margin-right:8px;\"\u003e•\u003c\/span\u003e\u003cspan\u003eThe incubation time of cells with drugs needs to be determined experimentally. Add subsequent reagents after drug stimulation of cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"margin-right:8px;\"\u003e3.\u003c\/span\u003e\u003cstrong\u003eDetection\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eMeasure using a TR-FRET-compatible microplate reader. Excitation wavelength: 320\/340nm, emission wavelengths: 620nm and 665nm.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#1655A5;\"\u003e\u003cstrong\u003e【Result Calculation】\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1) Calculate the signal value (Ratio): Multiply the 665nm fluorescence signal by the 620nm fluorescence signal and then by 10,000.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan\u003eRatio = (665\/620) × 10,000\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2) Calculate CV (%):\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan\u003eCV (%) = Standard Deviation \/ Mean Ratio × 100%\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#1655A5;\"\u003e\u003cstrong\u003e【Data Example】\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe following data is for reference only and may vary depending on the plate reader used.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"image_resized\" style=\"height:auto;margin-left:30px;vertical-align:middle;width:492px;\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/6b148df9-8915-4ecf-bd83-f9ed2ecadddb.png\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#FFFFFF;\"\u003e3 \/3\u003c\/span\u003e\u003cstrong\u003eNote: *Data is for reference only. Recommended microplate: 384-well plate, white, shallow well.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"100T","offer_id":42332771876939,"sku":"UA086127-100T","price":530.0,"currency_code":"USD","in_stock":true},{"title":"500T","offer_id":42332771909707,"sku":"UA086127-500T","price":1315.0,"currency_code":"USD","in_stock":true},{"title":"2500T","offer_id":42332771942475,"sku":"UA086127-2500T","price":4925.0,"currency_code":"USD","in_stock":true},{"title":"10000T","offer_id":42332771975243,"sku":"UA086127-10000T","price":13135.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_a428dd8d-e1fc-4445-b4e7-90041b16995d.png?v=1788530559"},{"product_id":"unione-tr-fret-human-cdk2crbn-protac-binding-kit-ua086128","title":"UniOne® TR-FRET Human CDK2\/CRBN PROTAC Binding Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-80℃\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eThe kit employs homogeneous time-resolved fluorescence resonance energy transfer (TR‑FRET) technology for the quantification of molecular glue.\u003c\/span\u003e\u003cspan\u003eCDK2 Degrader7\u003c\/span\u003e\u003cspan\u003eand mediated by the compound to be detected\u003c\/span\u003e\u003cspan\u003eCRBN\u003c\/span\u003e\u003cspan\u003e(\u003c\/span\u003e\u003cspan\u003eDDB1\/CRBN complex\u003c\/span\u003e\u003cspan\u003e)\u003c\/span\u003e\u003cspan\u003ewith\u003c\/span\u003e\u003cspan\u003eCDK2(\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eCDK2\/Cyclin E1 complex)\u003c\/span\u003e\u003cspan\u003eInteraction. This method is simple and fast to implement.\u003c\/span\u003e\u003cspan\u003eland\u003c\/span\u003e\u003cspan\u003eThe assay is capable of mediating the DDB1\/CRBN complex with\u003c\/span\u003e\u003cspan\u003eCDK2\u003c\/span\u003e\u003cspan\u003eHigh-throughput screening of small-molecule interactions.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eAs\u003c\/span\u003e\u003cspan\u003eThe following figure\u003c\/span\u003e\u003cspan\u003eAs shown,\u003c\/span\u003e\u003cspan\u003eCRBN\u003c\/span\u003e\u003cspan\u003eand\u003c\/span\u003e\u003cspan\u003eCDK2\u003c\/span\u003e\u003cspan\u003eThe interaction between them was detected using an Eu-labeled anti-Tag1 antibody (TR‑FRET donor) and an Ac‑labeled anti-Tag2 antibody (TR‑FRET acceptor). Due to the molecular glue\u003c\/span\u003e\u003cspan\u003eCDK2 Degrader7\u003c\/span\u003e\u003cspan\u003eMediated CRBN and\u003c\/span\u003e\u003cspan\u003eCDK2\u003c\/span\u003e\u003cspan\u003eof the interaction, when the donor antibody approaches the receptor antibody, excitation of the donor antibody triggers fluorescence resonance energy transfer (FRET) to the receptor antibody, causing the receptor antibody to emit a signal at a wavelength of 665 nm. This specific signal is correlated with\u003c\/span\u003e\u003cspan\u003eCDK2 Degrader7\u003c\/span\u003e\u003cspan\u003eand CRBN,\u003c\/span\u003e\u003cspan\u003eCDK2\u003c\/span\u003e\u003cspan\u003ethe extent of their interaction is directly proportional. This homogeneous assay is easy to perform and requires no washing.\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/6f64731c-0d3d-416d-ab75-2119270bb798.png\" style=\"margin-left:30px; width:538px; height:auto; vertical-align:middle;\"\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003eProduct Components\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003eand Storage Conditions]\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse:collapse;width:auto;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eComponents\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eConcentration\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e100T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e2500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e10000T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eStorage Temperature\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag1-\u003c\/span\u003e\u003cspan\u003eCRBN\u003c\/span\u003e\u003cspan\u003eprotein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003e0×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag2-\u003c\/span\u003e\u003cspan\u003eCDK2\u003c\/span\u003e\u003cspan\u003eprotein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCDK2 Degrader 7\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1mM\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e25\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAnti-Tag1 Eu antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003e0×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e250\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1m\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAnti-Tag2 Ac antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e25\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e125\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e40mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan\u003eNote: After the first thaw, aliquot immediately and store at the recommended temperature. Avoid storing diluted samples or subjecting them to repeated freeze-thaw cycles.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e,\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eReagent Preparation\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e.1\u003c\/span\u003e\u003cspan\u003eBefore use, allow all reagents to thaw at room temperature (equilibrate at room temperature for at least 30 minutes). The reaction volume in a 384‑well plate is 20 µL.\u003c\/span\u003e\u003cspan\u003emu\u003c\/span\u003e\u003cspan\u003eL (reagent volumes for the reaction system are shown in the table). Calculate the required reaction volume before preparation and prepare accordingly. The following formulation is for reference only, using 500 reactions as an example.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eTable 1\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003cspan\u003eReagent Preparation\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eReagent name\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eConfiguration\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eμL per test well\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003etake\u003c\/span\u003e\u003cspan\u003e2 mL 10\u003c\/span\u003e\u003cspan\u003etimes\u003c\/span\u003e\u003cspan\u003eDetection buffer1\u003c\/span\u003e\u003cspan\u003eJoin\u003c\/span\u003e\u003cspan\u003e18 mL deionized water, dilute\u003c\/span\u003e\u003cspan\u003eExplanation\u003c\/span\u003e\u003cspan\u003eTo\u003c\/span\u003e\u003cspan\u003e1×, mixed\u003c\/span\u003e\u003cspan\u003eEvenly prepared\u003c\/span\u003e\u003cspan\u003eUse\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCDK2 Degrader7\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eAccording to the reaction system, the compound is used with\u003c\/span\u003e\u003cspan\u003eDilute the 1× detection buffer to the desired concentration.\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag1-CRBN protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003etake\u003c\/span\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e0 μL\u003c\/span\u003e\u003cspan\u003eTag1-CRBN protein\u003c\/span\u003e\u003cspan\u003eStock solution, 1× Detection buffer diluted to 2 mL,\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eMix well and set aside.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag2-\u003c\/span\u003e\u003cspan\u003eCDK2\u003c\/span\u003e\u003cspan\u003eprotein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003etake\u003c\/span\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e0 μL\u003c\/span\u003e\u003cspan\u003eTag2-\u003c\/span\u003e\u003cspan\u003eCDK2\u003c\/span\u003e\u003cspan\u003eprotein\u003c\/span\u003e\u003cspan\u003eStock solution, diluted with 1× Detection buffer to 2 mL,\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003emix well and set aside.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAntibody Mix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:justify\"\u003e\u003cspan style=\"font-weight:bold\"\u003eTake\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e50\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eμL Anti-Tag1 Eu antibody\u003c\/span\u003e\u003cspan\u003estock solution, add\u003c\/span\u003e\u003cspan\u003e1× Detection buffer 2.4\u003c\/span\u003e\u003cspan\u003e50\u003c\/span\u003e\u003cspan\u003emL,\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e \u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003emix well;\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003etake\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e25\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eμL Anti-Tag2 Ac antibody\u003c\/span\u003e\u003cspan\u003estock solution, add\u003c\/span\u003e\u003cspan\u003e1× Detection buffer 2.\u003c\/span\u003e\u003cspan\u003e475\u003c\/span\u003e\u003cspan\u003emL,\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e \u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003emix well\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e;\u003c\/span\u003e\u003cspan\u003ethe two\u003c\/span\u003e\u003cspan\u003eare mixed 1:1 to form the Antibody Mix.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan\u003e1.2\u003c\/span\u003e\u003cspan\u003esample to be tested\u003c\/span\u003e\u003cspan\u003egradient dilution\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eusing\u003c\/span\u003e\u003cspan\u003eCDK2 Degrader7\u003c\/span\u003e\u003cspan\u003eas an example, the diluent is 1× Detection buffer,\u003c\/span\u003e\u003cspan\u003eto reduce the impact of matrix effect interference, it is recommended to use a solution with the same matrix as the sample being tested; and\u003c\/span\u003e\u003cspan\u003ethe sample to be tested should be adjusted according to its actual concentration.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eTable\u003c\/span\u003e\u003cspan\u003e2.\u003c\/span\u003e\u003cspan\u003eCDK2 Degrader7\u003c\/span\u003e\u003cspan\u003egradient dilution\u003c\/span\u003e\u003cspan\u003e(adjusted based on actual conditions)\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eCDK2 Degrader7\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003efinal concentration (nM)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eCDK2 Degrader7\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003ePreparation concentration (nM)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003ePreparation method\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e①\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-style:italic;text-decoration:underline;color:#000000\"\u003e1000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-style:italic;text-decoration:underline;color:#000000\"\u003e10000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003cspan\u003e1m\u003c\/span\u003e\u003cspan\u003eM\u003c\/span\u003e\u003cspan\u003eCDK2 Degrader7\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e99\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e②\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-style:italic;text-decoration:underline;color:#000000\"\u003e333.3333\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-style:italic;text-decoration:underline;color:#000000\"\u003e3333.333\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e0μL\u003c\/span\u003e\u003cspan\u003e①\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e40\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e③\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-style:italic;text-decoration:underline;color:#000000\"\u003e111.1111\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-style:italic;text-decoration:underline;color:#000000\"\u003e1111.111\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e0μL\u003c\/span\u003e\u003cspan\u003e②\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e40\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e④\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-style:italic;text-decoration:underline;color:#000000\"\u003e37.0370\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-style:italic;text-decoration:underline;color:#000000\"\u003e370.370\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e0μL\u003c\/span\u003e\u003cspan\u003e③\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e40\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑤\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-style:italic;text-decoration:underline;color:#000000\"\u003e12.3457\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-style:italic;text-decoration:underline;color:#000000\"\u003e123.457\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e0μL\u003c\/span\u003e\u003cspan\u003e④\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e40\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑥\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-style:italic;text-decoration:underline;color:#000000\"\u003e4.1152\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-style:italic;text-decoration:underline;color:#000000\"\u003e41.152\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e0μL\u003c\/span\u003e\u003cspan\u003e⑤\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e40\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑦\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-style:italic;text-decoration:underline;color:#000000\"\u003e1.3717\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-style:italic;text-decoration:underline;color:#000000\"\u003e13.717\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e0μL\u003c\/span\u003e\u003cspan\u003e⑥\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e40\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑧\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-style:italic;text-decoration:underline;color:#000000\"\u003e0.4572\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-style:italic;text-decoration:underline;color:#000000\"\u003e4.572\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e0μL\u003c\/span\u003e\u003cspan\u003e⑦\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e40\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑨\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-style:italic;text-decoration:underline;color:#000000\"\u003e0.1524\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-style:italic;text-decoration:underline;color:#000000\"\u003e1.524\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e0μL\u003c\/span\u003e\u003cspan\u003e⑧\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e40\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eBlank\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-style:italic;text-decoration:underline;color:#000000\"\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-style:italic;text-decoration:underline;color:#000000\"\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003cspan\u003e0μL of 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003e2\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e,\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eSample addition and controls\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003ePositive drug standard curve\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eSample to be tested\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eBlank\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eControl well\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNC\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eGradient dilution\u003c\/span\u003e\u003cspan\u003eCDK2 Degrader7\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eGradient-diluted sample to be tested\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"4\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e0\u003c\/span\u003e\u003cspan\u003eμL 1\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd\u003c\/span\u003e\u003cspan\u003e10μL Antibody Mix\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4μL\u003c\/span\u003e\u003cspan\u003eTag1-CRBN protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4μL\u003c\/span\u003e\u003cspan\u003eTag2-\u003c\/span\u003e\u003cspan\u003eCDK2\u003c\/span\u003e\u003cspan\u003eprotein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10μL Antibody Mix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"4\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eSeal the plate wells with a sealing film.\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e,\u003c\/span\u003e\u003cspan\u003eIncubate at room temperature for 2 hours;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan\u003e2.1\u003c\/span\u003e\u003cspan\u003eSample to be tested\u003c\/span\u003e\u003cspan\u003e: 4 μL\u003c\/span\u003e\u003cspan\u003eTag1-CRBN protein\u003c\/span\u003e\u003cspan\u003eworking solution, 4 μL\u003c\/span\u003e\u003cspan\u003eTag2-\u003c\/span\u003e\u003cspan\u003eCDK2\u003c\/span\u003e\u003cspan\u003eprotein\u003c\/span\u003e\u003cspan\u003eworking solution, 2μL\u003c\/span\u003e\u003cspan\u003egradient dilution\u003c\/span\u003e\u003cspan\u003esample to be tested\u003c\/span\u003e\u003cspan\u003e,\u003c\/span\u003e\u003cspan\u003e10μL of mixed Antibody Mix, added sequentially into the 384-well plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.2\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003epositive drug standard curve: 4μL\u003c\/span\u003e\u003cspan\u003eTag1-CRBN protein\u003c\/span\u003e\u003cspan\u003eworking solution, 4μL\u003c\/span\u003e\u003cspan\u003eTag2-\u003c\/span\u003e\u003cspan\u003eCDK2\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eprotein\u003c\/span\u003e\u003cspan\u003eworking solution\u003c\/span\u003e\u003cspan\u003e,\u003c\/span\u003e\u003cspan\u003e2μL gradient-diluted\u003c\/span\u003e\u003cspan\u003eCDK2 Degrader7\u003c\/span\u003e\u003cspan\u003e, 10μL Antibody Mix.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e.3\u003c\/span\u003e\u003cspan\u003eblank\u003c\/span\u003e\u003cspan\u003econtrol\u003c\/span\u003e\u003cspan\u003ewell\u003c\/span\u003e\u003cspan\u003e: 2μL of 1× Detection buffer instead\u003c\/span\u003e\u003cspan\u003eof the sample to be tested\u003c\/span\u003e\u003cspan\u003e;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.4\u003c\/span\u003e\u003cspan\u003eNC\u003c\/span\u003e\u003cspan\u003e: 1\u003c\/span\u003e\u003cspan\u003e0\u003c\/span\u003e\u003cspan\u003eμL 1\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003cspan\u003eadd\u003c\/span\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eAntibody Mix\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAfter adding all samples, centrifuge, seal with a plate cover, and incubate at room temperature for 2 hours.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e3,\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eDetection\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eDetect on an ELISA reader compatible with TR-FRET.\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003cspan\u003eThe excitation light is 320\/340 nm, and the emission wavelengths are detected at 620 nm and 665 nm.\u003c\/span\u003e\u003cspan\u003eEmission\u003c\/span\u003e\u003cspan\u003ewavelengths.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e[Result Calculation]\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1)\u003c\/span\u003e\u003cspan\u003eCalculate the signal value\u003c\/span\u003e\u003cspan\u003e(Ratio)\u003c\/span\u003e\u003cspan\u003e: Multiply the 665 nm fluorescence signal by the 620 nm fluorescence signal, then multiply by 10,000\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eRatio\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e=\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e(665\/620)\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003cspan\u003e10,000\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2)\u003c\/span\u003e\u003cspan\u003eCalculate based on the signal value\u003c\/span\u003e\u003cspan\u003eNet signal\u003c\/span\u003e\u003cspan\u003e:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eNet signal\u003c\/span\u003e\u003cspan\u003e= (Std-\u003c\/span\u003e\u003cspan\u003eNC\u003c\/span\u003e\u003cspan\u003e)\/\u003c\/span\u003e\u003cspan\u003eNC\u003c\/span\u003e\u003cspan\u003e×100\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3)\u003c\/span\u003e\u003cspan\u003eCalculate CV (%)\u003c\/span\u003e\u003cspan\u003e:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eCV (%) = Standard Deviation \/ Mean Ratio × 100%\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e[[Data Example]\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe following data cannot replace the data obtained in experiments; it is only for illustration, and results may\u003c\/span\u003e\u003cspan\u003evary depending on the plate reader\u003c\/span\u003e\u003cspan\u003eand\u003c\/span\u003e\u003cspan\u003eequipment.\u003c\/span\u003e  \u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/951e80f9-7316-42ea-9bf5-57e4b518838f.png\" style=\"margin-left:30px; width:424px; height:auto; vertical-align:middle;\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/7473c47a-7f85-4425-8b40-65e58f9f00ed.png\" style=\"margin-left:30px; width:277px; height:auto; vertical-align:middle;\"\u003e\u003c\/p\u003e\n\u003cspan\u003e \u003c\/span\u003e\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/b3ffcf38-ad79-4f80-8abb-8fbd6134ac93.png\" style=\"margin-left:30px; width:343px; height:auto; vertical-align:middle;\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003eNote: Recommended\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eMicroplate (384-well plate, white, shallow wells)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"100T","offer_id":42332773613643,"sku":"UA086128-100T","price":730.0,"currency_code":"USD","in_stock":true},{"title":"500T","offer_id":42332773646411,"sku":"UA086128-500T","price":2180.0,"currency_code":"USD","in_stock":true},{"title":"2500T","offer_id":42332773679179,"sku":"UA086128-2500T","price":8175.0,"currency_code":"USD","in_stock":true},{"title":"10000T","offer_id":42332773711947,"sku":"UA086128-10000T","price":21800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_8e449840-3ecd-4cdf-8436-93b277c07154.png?v=1788530621"},{"product_id":"human-fc-quantification-kit-human-fc-ua085005","title":"Human Fc quantification kit - Human Fc quantitative kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-80℃\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003eThis kit employs homogeneous time-resolved fluorescence technology and can be used to detect Human IgG antibodies in cell culture supernatants. The kit includes an antibody-labeled donor Eu (anti-Human IgG-Eu) and a Human IgG-labeled acceptor (Human IgG-Ac). The anti-Human IgG-Eu binds to Human IgG-Ac, and when excited by 320 nm light, the donor transfers energy to the acceptor, which then emits light at a specific wavelength (665 nm).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003eHuman IgG antibodies in the sample competitively bind to anti-Human IgG-Eu, resulting in a signal inversely proportional to the concentration of Human IgG in the standard. This allows quantification of Human IgG in the sample based on a standard curve. This homogeneous assay requires no washing and enables accurate quantification of total Human IgG.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/6c92386a-5f2d-487b-af66-4f92000fe773.jpeg\" style=\"margin-left:30px; width:661px; height:auto; vertical-align:middle;\" alt=\"\"\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003ctable border=\"1\" style=\"border-collapse:collapse;width:auto;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eComponent\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eConcentration\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e100T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e2500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"\"\u003e10000T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eStorage Temperature\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAnti-Human IgG-Eu*\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e250μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e1000μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eHuman IgG-Ac*\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e250μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e1000μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIgG Standard\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1mg\/ml\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:极align:center\"\u003e\u003cspan\u003e10μL\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e200μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #极000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAssay Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #极000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1.5mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e6ml\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e30mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e120mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eStandard Diluent\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1.5mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e6ml\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:极1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e30mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e120mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan\u003eNote: Aliquot immediately after first thaw and store at recommended temperatures. Avoid storage after dilution or repeated freeze-thaw cycles.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cstrong\u003e1. Reagent Preparation\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThaw all reagents at room temperature before use (equilibrate at room temperature for at least 30 min). The reaction volume for the 384-well shallow plate is 20 μL (reagent volumes for the reaction system are shown in Table 2). Calculate the required volume for the experiment before preparation and prepare as needed; the following preparation is for reference only, using 500 assays as an example.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan\u003eTable 1. Reagent Preparation\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:1px solid #000000;width:auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eReagent Name\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003ePreparation\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eVolume per Assay Well (μL)\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eAnti-Human IgG-Eu\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eAdd 2450 μL Detection Buffer to 50 μL Anti-Human IgG-Eu, mix well, and keep on standby.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003e5\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eHuman IgG-Ac\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eAdd 2450 μL Detection Buffer to 50 μL Human IgG-Ac, mix well, and keep on standby.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003e5\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eIgG Standard\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eTake 10 μL of the standard stock solution, add 115 μL of standard diluent, mix thoroughly. Standard curve dilutions are as follows.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003e10\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan\u003eTable 2. Preparation of Standard Working Solutions\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:1px solid #000000;width:auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\n\u003cp\u003e\u003cspan\u003eStandard Working Solution Concentration\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e(ng\/mL)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003ePreparation Method\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e①\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cspan\u003e80000.0\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\n\u003cspan\u003e10\u003c\/span\u003e\u003cspan style=\"color:#000000;\"\u003eμ\u003c\/span\u003e\u003cspan\u003eL standard original stock solution + 115\u003c\/span\u003e\u003cspan style=\"color:#000000;\"\u003eμ\u003c\/span\u003e\u003cspan\u003eL standard diluent\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e②\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cspan\u003e20000.0\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\n\u003cspan\u003e20\u003c\/span\u003e\u003cspan style=\"color:#000000;\"\u003eμ\u003c\/span\u003e\u003cspan\u003eL \u003c\/span\u003e\u003cspan style=\"color:#000000;\"\u003e①\u003c\/span\u003e\u003cspan\u003e+60\u003c\/span\u003e\u003cspan style=\"color:#000000;\"\u003eμ\u003c\/span\u003e\u003cspan\u003eL standard diluent\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e③\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cspan\u003e5000.0\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\n\u003cspan\u003e20\u003c\/span\u003e\u003cspan style=\"color:#000000;\"\u003eμ\u003c\/span\u003e\u003cspan\u003eL \u003c\/span\u003e\u003cspan style=\"color:#000000;\"\u003e②\u003c\/span\u003e\u003cspan\u003e+60\u003c\/span\u003e\u003cspan style=\"color:#000000;\"\u003eμ\u003c\/span\u003e\u003cspan\u003eL standard diluent\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e④\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cspan\u003e1250.0\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\n\u003cspan\u003e20\u003c\/span\u003e\u003cspan style=\"color:#000000;\"\u003eμ\u003c\/span\u003e\u003cspan\u003eL ③+60\u003c\/span\u003e\u003cspan style=\"color:#000000;\"\u003eμ\u003c\/span\u003e\u003cspan\u003eL standard diluent\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e⑤\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cspan\u003e312.5\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\n\u003cspan\u003e20\u003c\/span\u003e\u003cspan style=\"color:#000000;\"\u003eμ\u003c\/span\u003e\u003cspan\u003eL ④+60\u003c\/span\u003e\u003cspan style=\"color:#000000;\"\u003eμ\u003c\/span\u003e\u003cspan\u003eL standard diluent\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e⑥\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cspan\u003e78.125\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\n\u003cspan\u003e20\u003c\/span\u003e\u003cspan style=\"color:#000000;\"\u003eμ\u003c\/span\u003e\u003cspan\u003eL ⑤+60\u003c\/span\u003e\u003cspan style=\"color:#000000;\"\u003eμ\u003c\/span\u003e\u003cspan\u003eL standard diluent\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e⑦\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cspan\u003e19.531\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\n\u003cspan\u003e20\u003c\/span\u003e\u003cspan style=\"color:#000000;\"\u003eμ\u003c\/span\u003e\u003cspan\u003eL ⑥+60\u003c\/span\u003e\u003cspan style=\"color:#000000;\"\u003eμ\u003c\/span\u003e\u003cspan\u003eL standard diluent\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e⑧\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cspan\u003e4.88\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\n\u003cspan\u003e20\u003c\/span\u003e\u003cspan style=\"color:#000000;\"\u003eμ\u003c\/span\u003e\u003cspan\u003eL ⑦+60\u003c\/span\u003e\u003cspan style=\"color:#000000;\"\u003eμ\u003c\/span\u003e\u003cspan\u003eL standard diluent\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e⑨\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cspan\u003e1.220\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\n\u003cspan\u003e20\u003c\/span\u003e\u003cspan style=\"color:#000000;\"\u003eμ\u003c\/span\u003e\u003cspan\u003eL ⑧+60\u003c\/span\u003e\u003cspan style=\"color:#000000;\"\u003eμ\u003c\/span\u003e\u003cspan\u003eL standard diluent\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e⑩\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cspan\u003e0.305\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\n\u003cspan\u003e20\u003c\/span\u003e\u003cspan style=\"color:#000000;\"\u003eμ\u003c\/span\u003e\u003cspan\u003eL ⑨+60\u003c\/span\u003e\u003cspan style=\"color:#000000;\"\u003eμ\u003c\/span\u003e\u003cspan\u003eL standard diluent\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003eBlank\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cspan\u003e0.0\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\n\u003cspan\u003e60\u003c\/span\u003e\u003cspan style=\"color:#000000;\"\u003eμ\u003c\/span\u003e\u003cspan\u003eL standard diluent\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2. Sample Addition and Controls\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003eOrder of sample addition: 10\u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan style=\"color:#000000;\"\u003eL standard or sample; 5\u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan style=\"color:#000000;\"\u003eL Anti-Human IgG-Eu; 5\u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan style=\"color:#000000;\"\u003eL Human IgG-Ac are added sequentially to the 384-well shallow plate. (Note: Human IgG-Ac is added last)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003eNC: Replace 5\u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan style=\"color:#000000;\"\u003eL Human IgG-Ac with 5\u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan style=\"color:#000000;\"\u003eL Detection Buffer, and replace sample with 10\u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan style=\"color:#000000;\"\u003eL standard diluent.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003eMaximum Control: Replace sample with 10\u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan style=\"color:#000000;\"\u003eL standard diluent.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"margin-right:8px;\"\u003e3.\u003c\/span\u003e\u003cstrong\u003eDetection\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000;\"\u003eSeal the plate wells with a plate sealer, centrifuge at 1000 rpm for 1 min, and incubate at room temperature for 1 h. Detect on a TR-FRET compatible microplate reader (excitation wavelength 320 nm, emission wavelengths 620 nm and 665 nm).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#1655A5;\"\u003e\u003cstrong\u003e[Result Calculation]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1. TR-FRET Ratio Calculation\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eTR-FRET Ratio = 665nm \/ 620nm *10000\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2. Calculate CV (%)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eCV（%）= Standard Deviation\/Mean Ratio×100%\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#1655A5;\"\u003e\u003cstrong\u003e[Data Example]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003ePlease note that the following data cannot replace the data obtained in experiments and is provided only as an example. Results may vary depending on the TR-FRET compatible instrument.\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:1px solid #000000;width:auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" rowspan=\"2\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003eStd\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" rowspan=\"2\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003e \u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003eConcentration (ng\/mL)\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"2\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003eStandard\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003eRatio\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003eCV%\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e①\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e80000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e163.65\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e7.65%\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e②\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e20000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e255.25\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e0.86%\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e③\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e5000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e467.2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e0.29%\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e④\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e1250\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e1581\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e6.71%\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e⑤\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e312.5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e3420.5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e4.53%\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e⑥\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e78.125\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e3980.1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e3.49%\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e⑦\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e19.531\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e3704.5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e0.02%\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e⑧\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e4.883\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e3785.5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e3.90%\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e⑨\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e1.221\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e3673.5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e1.56%\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e⑩\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e0.305\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e3940.5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e8.06%\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eBlank\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e3965\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e3.92%\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eNC\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eNC\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e121.75\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e5.52%\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"image_resized\" style=\"height:auto;margin-left:30px;vertical-align:middle;width:387px;\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/c0bcf475-af00-4450-b37a-4caf6d62aafd.png\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote: Recommended microplate (384-well plate, white, shallow well)\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"100T","offer_id":42558366416971,"sku":"UA085005-100T","price":300.0,"currency_code":"USD","in_stock":true},{"title":"500T","offer_id":42558366449739,"sku":"UA085005-500T","price":996.67,"currency_code":"USD","in_stock":true},{"title":"2500T","offer_id":42558366482507,"sku":"UA085005-2500T","price":2951.67,"currency_code":"USD","in_stock":true},{"title":"10000T","offer_id":42558366515275,"sku":"UA085005-10000T","price":5425.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_0cd1baec-281d-4b2c-90bb-3b20a483edb6.png?v=1788519762"},{"product_id":"camp-stimulation-buffer-1xibmx-ua086057","title":"cAMP Stimulation buffer (1X) containing IBMX","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore at 2-8℃ in the dark for 12 months\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"line-height:150%;margin:11.5pt 36.0pt .0001pt 51.0pt;text-autospace:ideograph-numeric ideograph-other;text-indent:20.0pt;\"\u003e\u003cspan style=\"font-size:10.0pt;line-height:150%;\"\u003eThis reagent can be used as a supplement to the \u003c\/span\u003e\u003cspan style=\"font-size:10.0pt;line-height:150%;\" lang=\"EN-US\"\u003eUniOne® TR-FRET cAMP HiRange Assay Kit\u003c\/span\u003e\u003cspan style=\"font-size:10.0pt;line-height:150%;\"\u003e (\u003c\/span\u003e\u003cspan style=\"font-size:10.0pt;line-height:150%;\" lang=\"EN-US\"\u003eUA086053\u003c\/span\u003e\u003cspan style=\"font-size:10.0pt;line-height:150%;\"\u003e). The reagent contains \u003c\/span\u003e\u003cspan style=\"font-size:10.0pt;line-height:150%;\" lang=\"EN-US\"\u003eIBMX\u003c\/span\u003e\u003cspan style=\"font-size:10.0pt;line-height:150%;\"\u003e to inhibit \u003c\/span\u003e\u003cspan style=\"font-size:10.0pt;line-height:150%;\" lang=\"EN-US\"\u003ePDE\u003c\/span\u003e\u003cspan style=\"font-size:10.0pt;line-height:150%;\"\u003e activity and maintain \u003c\/span\u003e\u003cspan style=\"font-size:10.0pt;line-height:150%;\" lang=\"EN-US\"\u003ecAMP\u003c\/span\u003e\u003cspan style=\"font-size:10.0pt;line-height:150%;\"\u003e levels. It should be selected based on experimental purposes.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eGuidelines\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"5ml","offer_id":42558367137867,"sku":"UA086057-5ml","price":40.0,"currency_code":"USD","in_stock":true},{"title":"15ml","offer_id":42558367170635,"sku":"UA086057-15ml","price":120.0,"currency_code":"USD","in_stock":true},{"title":"70ml","offer_id":42558367203403,"sku":"UA086057-70ml","price":357.0,"currency_code":"USD","in_stock":true},{"title":"220ml","offer_id":42558367236171,"sku":"UA086057-220ml","price":715.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_a63ef11b-f667-45d7-aa1f-b0e5134f7c9b.png?v=1788523312"},{"product_id":"unione-tr-fret-human-brd4bd2h4-binding-kit-ua086144","title":"UniOne® TR-FRET Human BRD4(BD2)\/H4 Binding Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e【\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003eASSAY PRINCIPLE\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e】\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eThe kit is based on homogeneous time-resolved fluorescence technology (TR-FRET) technology，which is used to assess the molecular-mediated interaction between the BRD4 (BD2) and the H4 peptide. This method provides a high-throughput approach to detect the small molecules capable of mediating the interaction between BRD4 (BD2) and H4 peptide simply and rapidly.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eAs shown in the figure below, the interaction between BRD4 (BD2) and H4 peptide was detected by an Eu-labeled anti-Tag1 antibody (TR-FRET donor) and an Ac-labeled anti-Tag2 antibody (TR-FRET receptor). The donor is in close proximity to the receptor antibody，excitation of the donor antibody triggers fluorescence resonance energy transfer (FRET) to the receptor antibody, resulting in specific emission of a signal at a wavelength of 665 nm from the receptor antibody. Since the molecular (+)\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eJQ-1 can block the interaction between BRD4 (BD2) and H4 peptide, \u003c\/span\u003e\u003cspan\u003ethe FRET signal is reduced.\u003c\/span\u003e\u003cspan\u003e This specific signal is proportional to the degree of interaction between BRD4 (BD2) and H4 peptide. This homogeneous assay is simple to perform and requires no washing steps.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003e            \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/286e88ea-ec7d-4790-8802-e1716f1f439f.png\" style=\"margin-left:30px; width:522px; height:auto; vertical-align:middle;\" alt=\"\"\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e【Composition and Storage Conditions】\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse:collapse;width:auto;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eComposition \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003econcentration\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e100T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e2500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e10000T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eStorage \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag\u003c\/span\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e-\u003c\/span\u003e\u003cspan\u003eBRD4 (BD2)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag\u003c\/span\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e-\u003c\/span\u003e\u003cspan\u003eH4 peptide\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAnti-Tag\u003c\/span\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e Eu antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003e0×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e25\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e125\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAnti-Tag\u003c\/span\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e Ac antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e12.5\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e40\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e200\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1m\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003cspan\u003em\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e0\u003c\/span\u003e\u003cspan\u003emL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003e0\u003c\/span\u003e\u003cspan\u003emL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e40\u003c\/span\u003e\u003cspan\u003e0\u003c\/span\u003e\u003cspan\u003emL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eNote: Aliquot into appropriate volumes, store at the recommended temperature and avoid repeated freeze-thaw cycle.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"color:#1655A5;\"\u003e\u003cstrong\u003e【Process and Operations】\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e1、Reagent Preparation\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e1.1 Compositions equilibration\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify;\"\u003e\u003cspan\u003eBefore use, dissolve all reagents at room temperature (allow at least 30 minutes for temperature equilibration). For the 384-well plate assay, use 20 μL of reaction mixture (the reagent quantities are listed in the table below). Calculate the required volume prior to preparation accordingly. The following preparation steps are provided for reference only, using a 500-well plate as an example.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan\u003eTable 1. Reagent Preparation\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:1px solid #000000;width:auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eConfiguration\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eμL\/well\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eTag1-BRD4 (BD2)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:justify;\"\u003e\u003cspan\u003eTake 20 μL of the 100× Tag1-BRD4 (BD2) stock solution, add 1.98 mL of Detection buffer to dilute it to 1× concentration, and mix thoroughly for later use.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eTag2-H4 peptide\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:justify;\"\u003e\u003cspan\u003eTake 20 μL of 100× Tag2-H4 peptide, add it to 1.98 mL of Detection buffer to dilute to 1× concentration, and mix thoroughly for later use.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eAntibody Mix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eTake 25 μL of the 100× Anti-Tag1 Eu antibody stock solution, add 2475 μL of Detection buffer to dilute to 2.5 mL, and mix thoroughly; take 200 μL of the 12.5× Anti-Tag2 Ac antibody stock solution, add 2300 μL of Detection buffer to dilute to 2.5 mL, and mix thoroughly; combine the two solutions in a 1:1 ratio to obtain the Antibody Mix.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e1.2 Gradient dilution of the sample\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify;\"\u003e\u003cspan\u003e(+) JQ-1 as an example, the dilution solution is Detection buffer. To minimize interference from matrix effects, it is recommended to use a solution with the same matrix composition as the sample being tested; additionally, the sample concentration should be adjusted according to its actual situation.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan\u003eTable 2. (+) JQ-1 gradient dilution（Adjust according to the actual situation）\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:1px solid #000000;width:auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003e(+) JQ-1 final concentration（nM）\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003e(+) JQ-1 preparation concentration（nM）\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eDilution\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e①\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e300\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e3000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e0.5μL 500 μM (+) JQ1 + 82.85μL Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e②\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e100.00\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e1000.00\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL ① +20μL Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e③\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e33.33\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e333.33\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL ② +20μL Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e④\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e11.11\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e111.11\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL ③ +20μL Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑤\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e3.70\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e37.04\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL ④ +20μL Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑥\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e1.23\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e12.35\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL ⑤ +20μL Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑦\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e0.41\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e4.12\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL ⑥ +20μL Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑧\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e0.14\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e1.37\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL ⑦+20μL Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑨\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e0.05\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e0.46\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL ⑧+20μL Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑩\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e0.02\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e0.15\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL ⑨+20μL Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eBlank\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e20μL Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2、Sample and control\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify;\"\u003e\u003cstrong\u003e2.1 Sample：\u003c\/strong\u003e\u003cspan\u003eAdd 4 μL of Tag1-BRD4 (BD2) working solution, 4 μL of Tag2-H4 peptide working solution, 2 μL of the gradient-diluted sample , and 10 μL of the mixed Antibody Mix sequentially into the 384-well plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify;\"\u003e\u003cstrong\u003e2.2 (+) JQ-1 : \u003c\/strong\u003e\u003cspan\u003e4 μL of Tag1-BRD4 (BD2) working solution, 4 μL of Tag2-H4 peptide working solution, 2 μL of gradient-diluted (+) JQ-1\u003c\/span\u003e\u003cstrong\u003e \u003c\/strong\u003e\u003cspan\u003e, and 10 μL of the mixed Antibody Mix sequentially into the 384-well plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify;\"\u003e\u003cstrong\u003e2.3 Blank：\u003c\/strong\u003e\u003cspan\u003e4 μL of Tag1-BRD4 (BD2) working solution, 4 μL of Tag2-H4 peptide working solution, 2 μL of Detection buffer, and 10 μL of the mixed Antibody Mix sequentially into the 384-well plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify;\"\u003e\u003cstrong\u003e2.3 Negative control：\u003c\/strong\u003e\u003cspan\u003e10μL Detection buffer, and 10 μL of the mixed Antibody Mix sequentially into the 384-well plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify;\"\u003e\u003cspan\u003eAfter adding all samples, centrifuge, seal with a cover membrane, and incubate at room temperature for 2 hours.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan\u003eTable 3. Sample and control spotting process\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:1px solid #000000;width:auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003e(+)JQ-1\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eSample\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eBlank\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eNegative control(NC)\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003egradient-diluted (+) JQ-1\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003egradient-diluted sample\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" rowspan=\"5\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL Detection buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eand 10μL Antibody Mix\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"3\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e4μL Tag1-BRD4 (BD2)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"3\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eIncubate at room temperature for 10min\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"3\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e4μL Tag2-H4 peptide\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"3\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL Antibody Mix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"4\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eSeal the plate wells with a sealing film and incubate at room temperature for 2 hours\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"margin-right:8px;\"\u003e3、\u003c\/span\u003e\u003cstrong\u003eRead Data\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe detection was performed on a TR-FRET microplate reader. The excitation wavelength was 320\/340 nm, and the emission wavelengths at 620 nm and 665 nm were detected.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#1655A5;\"\u003e\u003cstrong\u003e【DATA REDUCTION 】\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1) Ratio：\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan\u003eRatio = (665\/620) ×10000\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2) Net signal：\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan\u003eNet signal = (Std-NC)\/NC×100\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eStd：the ratio of sample and control\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eNC: the ratio of negative control\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3) CV（%）：\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify;\"\u003e\u003cspan\u003eCV（%）= Standard Deviation\/Mean Ratio × 100%\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify;\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:justify;\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:justify;\"\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#1655A5;\"\u003e\u003cstrong\u003e【Data Example】\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan\u003eThe following data cannot replace experimental results obtained in experiments; they are provided solely for illustrative purposes, and outcomes may vary depending on the plate reader used.\u003c\/span\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"image_resized\" style=\"height:auto;margin-left:30px;vertical-align:middle;width:323px;\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/5a3877ea-5ad7-4fb5-af07-3d963bcb83ff.png\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"image_resized\" style=\"height:auto;margin-left:30px;vertical-align:middle;width:320px;\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/963c4956-5bbc-4afe-b924-157ede8d1335.png\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#FFFFFF;\"\u003e\/\u003c\/span\u003e\u003cstrong\u003eNote: Recommended microplate (384-well plate, white, shallow wells).\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"100T","offer_id":42883379331147,"sku":"UA086144-100T","price":730.0,"currency_code":"USD","in_stock":true},{"title":"500T","offer_id":42883379363915,"sku":"UA086144-500T","price":2180.0,"currency_code":"USD","in_stock":true},{"title":"2500T","offer_id":42883379396683,"sku":"UA086144-2500T","price":8175.0,"currency_code":"USD","in_stock":true},{"title":"10000T","offer_id":42883379429451,"sku":"UA086144-10000T","price":21800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_a08c2c06-1d15-48b6-b380-aa9c820e759f.png?v=1788534142"},{"product_id":"unione-tr-fret-human-brd4bd1h4-binding-kit-ua086143","title":"UniOne® TR-FRET Human BRD4(BD1)\/H4 Binding Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e【\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003eASSAY PRINCIPLE\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e】\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eThe kit is based on homogeneous time-resolved fluorescence technology (TR-FRET) technology，which is used to assess the molecular-mediated interaction between the BRD4 (BD1) and the H4 peptide. This method provides a high-throughput approach to detect the small molecules capable of mediating the interaction between BRD4 (BD1) and H4 peptide simply and rapidly.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eAs shown in the figure below, the interaction between BRD4 (BD1) and H4 peptide was detected by an Eu-labeled anti-Tag1 antibody (TR-FRET donor) and an Ac-labeled anti-Tag2 antibody (TR-FRET receptor). The donor is in close proximity to the receptor antibody，excitation of the donor antibody triggers fluorescence resonance energy transfer (FRET) to the receptor antibody, resulting in specific emission of a signal at a wavelength of 665 nm from the receptor antibody. Since the molecular (+)\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eJQ-1 block the interaction between BRD4 (BD1) and H4 peptide, \u003c\/span\u003e\u003cspan\u003ethe FRET signal is reduced.\u003c\/span\u003e\u003cspan\u003e This specific signal is proportional to the degree of interaction between BRD4\u003c\/span\u003e\u003cspan\u003e(\u003c\/span\u003e\u003cspan\u003eBD1)and H4 peptide. This homogeneous assay is simple to perform and requires no washing steps.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003e             \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/1ed1a1fa-e05f-4b92-81a8-cfdd67549457.png\" style=\"margin-left:30px; width:523px; height:auto; vertical-align:middle;\" alt=\"\"\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e【Composition and Storage Conditions】\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse:collapse;width:auto;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eComposition \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003econcentration\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e100T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e2500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e10000T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eStorage \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag\u003c\/span\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e-\u003c\/span\u003e\u003cspan\u003eBRD4 (BD1)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag\u003c\/span\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e-\u003c\/span\u003e\u003cspan\u003eH4 peptide\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAnti-Tag\u003c\/span\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e Eu antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003e0×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e25\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e125\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAnti-Tag\u003c\/span\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e Ac antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e12.5\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e40\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e200\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1m\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003cspan\u003em\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e0\u003c\/span\u003e\u003cspan\u003emL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003e0\u003c\/span\u003e\u003cspan\u003emL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e40\u003c\/span\u003e\u003cspan\u003e0\u003c\/span\u003e\u003cspan\u003emL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eNote: Aliquot into appropriate volumes, store at the recommended temperature and avoid repeated freeze-thaw cycle.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"color:#1655A5;\"\u003e\u003cstrong\u003e【Process and Operations】\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e1、Reagent Preparation\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e1.1 Compositions equilibration\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify;\"\u003e\u003cspan\u003eBefore use, dissolve all reagents at room temperature (allow at least 30 minutes for temperature equilibration). For the 384-well plate assay, use 20 μL of reaction mixture (the reagent quantities are listed in the table below). Calculate the required volume prior to preparation accordingly. The following preparation steps are provided for reference only, using a 500-well plate as an example.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan\u003eTable 1. Reagent Preparation\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:1px solid #000000;width:auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eConfiguration\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eμL\/well\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eTag1-BRD4 (BD1)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:justify;\"\u003e\u003cspan\u003eTake 20 μL of the 100× Tag1-BRD4 (BD1) stock solution, add 1.98 mL of Detection buffer to dilute it to 1× concentration, and mix thoroughly for later use.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eTag2-H4 peptide\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:justify;\"\u003e\u003cspan\u003eTake 20 μL of 100× Tag2-H4 peptide, add it to 1.98 mL of Detection buffer to dilute to 1× concentration, and mix thoroughly for later use.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eAntibody Mix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eTake 25 μL of the 100× Anti-Tag1 Eu antibody stock solution, add 2475 μL of Detection buffer to dilute to 2.5 mL, and mix thoroughly; take 200 μL of the 12.5× Anti-Tag2 Ac antibody stock solution, add 2300 μL of Detection buffer to dilute to 2.5 mL, and mix thoroughly; combine the two solutions in a 1:1 ratio to obtain the Antibody Mix.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e1.2 Gradient dilution of the sample\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify;\"\u003e\u003cspan\u003e(+) JQ-1 as an example, the dilution solution is Detection buffer. To minimize interference from matrix effects, it is recommended to use a solution with the same matrix composition as the sample being tested; additionally, the sample concentration should be adjusted according to its actual situation.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan\u003eTable 2. (+) JQ-1 gradient dilution（Adjust according to the actual situation）\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:1px solid #000000;width:auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003e(+) JQ-1 final concentration（nM）\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003e(+) JQ-1 preparation concentration（nM）\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eDilution\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e①\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e300\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e3000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e0.5μL 500 μM (+)JQ1 + 82.85μL Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e②\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e100.00\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e1000.00\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL ① +20μL Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e③\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e33.33\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e333.33\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL ② +20μL Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e④\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e11.11\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e111.11\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL ③ +20μL Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑤\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e3.70\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e37.04\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL ④ +20μL Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑥\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e1.23\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e12.35\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL ⑤ +20μL Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑦\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e0.41\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e4.12\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL ⑥ +20μL Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑧\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e0.14\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e1.37\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL ⑦+20μL Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑨\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e0.05\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e0.46\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL ⑧+20μL Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑩\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e0.02\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e0.15\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL ⑨+20μL Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eBlank\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e20μL Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2、Sample and control\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify;\"\u003e\u003cstrong\u003e2.1 Sample：\u003c\/strong\u003e\u003cspan\u003eAdd 4 μL of Tag1-BRD4 (BD1) working solution, 4 μL of Tag2-H4 peptide working solution, 2 μL of the gradient-diluted sample , and 10 μL of the mixed Antibody Mix sequentially into the 384-well plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify;\"\u003e\u003cstrong\u003e2.2 (+) JQ-1 : \u003c\/strong\u003e\u003cspan\u003e4 μL of Tag1-BRD4 (BD1) working solution, 4 μL of Tag2-H4 peptide working solution, 2 μL of gradient-diluted (+) JQ-1\u003c\/span\u003e\u003cstrong\u003e \u003c\/strong\u003e\u003cspan\u003e, and 10 μL of the mixed Antibody Mix sequentially into the 384-well plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify;\"\u003e\u003cstrong\u003e2.3 Blank：\u003c\/strong\u003e\u003cspan\u003e4 μL of Tag1-BRD4 (BD1) working solution, 4 μL of Tag2-H4 peptide working solution,, 2 μL of Detection buffer, and 10 μL of the mixed Antibody Mix sequentially into the 384-well plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify;\"\u003e\u003cstrong\u003e2.3 Negative control：\u003c\/strong\u003e\u003cspan\u003e10μL Detection buffer, and 10 μL of the mixed Antibody Mix sequentially into the 384-well plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify;\"\u003e\u003cspan\u003eAfter adding all samples, centrifuge, seal with a cover membrane, and incubate at room temperature for 2 hours.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan\u003eTable 3. Sample and control spotting process\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:1px solid #000000;width:auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003e(+) JQ-1\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eSample\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eBlank\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eNegative control (NC)\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003egradient-diluted (+) JQ-1\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003egradient-diluted sample\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" rowspan=\"5\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL Detection buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eand 10μL Antibody Mix\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"3\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e4μL Tag1-BRD4 (BD1)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"3\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eIncubate at room temperature for 10min\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"3\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e4μL Tag2-H4 peptide\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"3\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL Antibody Mix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"4\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eSeal the plate wells with a sealing film and incubate at room temperature for 2 hours.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"margin-right:8px;\"\u003e3、\u003c\/span\u003e\u003cstrong\u003eRead Data\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe detection was performed on a TR-FRET microplate reader. The excitation wavelength was 320\/340 nm, and the emission wavelengths at 620 nm and 665 nm were detected.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#1655A5;\"\u003e\u003cstrong\u003e【DATA REDUCTION 】\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1) Ratio：\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan\u003eRatio = (665\/620) ×10000\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2) Net signal：\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan\u003eNet signal = (Std-NC)\/NC×100\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eStd：the ratio of sample and control\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eNC: the ratio of negative control\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3) CV（%）：\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify;\"\u003e\u003cspan\u003eCV（%）= Standard Deviation\/Mean Ratio × 100%\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify;\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:justify;\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:justify;\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:justify;\"\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#1655A5;\"\u003e\u003cstrong\u003e【Data Example】\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan\u003eThe following data cannot replace experimental results obtained in experiments; they are provided solely for illustrative purposes, and outcomes may vary depending on the plate reader used.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"image_resized\" style=\"height:auto;margin-left:30px;vertical-align:middle;width:278px;\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/6207e499-89d8-4f2a-bdb2-fba3412c0ea9.png\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"image_resized\" style=\"height:auto;margin-left:30px;vertical-align:middle;width:317px;\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/092b56ae-cd8e-4f9a-853a-3365750bae6c.png\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote: Recommended microplate (384-well plate, white, shallow wells).\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"100T","offer_id":42883379462219,"sku":"UA086143-100T","price":730.0,"currency_code":"USD","in_stock":true},{"title":"500T","offer_id":42883379494987,"sku":"UA086143-500T","price":2180.0,"currency_code":"USD","in_stock":true},{"title":"2500T","offer_id":42883379527755,"sku":"UA086143-2500T","price":8175.0,"currency_code":"USD","in_stock":true},{"title":"10000T","offer_id":42883379560523,"sku":"UA086143-10000T","price":21800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_363cf2a6-6492-4c61-8ca7-10f64e405aa6.png?v=1788534138"},{"product_id":"unione-tr-fret-human-il23il23r-binding-kit-ua086129","title":"UniOne® TR-FRET Human IL23\/IL23R Binding Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-80℃\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003eThe kit utilizes homogeneous time-resolved fluorescence technology (TR-FRET) to measure the interaction between \u003c\/span\u003e\u003cspan\u003eIL23 (IL-23 alpha \u0026amp; IL-12 beta Heterodimer)\u003c\/span\u003e\u003cspan\u003e and \u003c\/span\u003e\u003cspan\u003eIL23R\u003c\/span\u003e\u003cspan\u003e. This method enables simple, rapid, and high-throughput screening of inhibitors and antibody blockers.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAs shown in the figure, the interaction between \u003c\/span\u003e\u003cspan\u003eIL23\u003c\/span\u003e\u003cspan\u003e and \u003c\/span\u003e\u003cspan\u003eIL23R\u003c\/span\u003e\u003cspan\u003e is detected using \u003c\/span\u003e\u003cspan\u003eEu-labeled anti-Tag1 antibody (TR-FRET donor) and Ac-labeled anti-Tag2 antibody (TR-FRET acceptor). The binding of \u003c\/span\u003e\u003cspan\u003eIL23\u003c\/span\u003e\u003cspan\u003e to \u003c\/span\u003e\u003cspan\u003eIL23R\u003c\/span\u003e\u003cspan\u003e brings the donor and acceptor antibodies into proximity, allowing the donor's excitation to trigger fluorescence resonance energy transfer (FRET) to the acceptor, resulting in a specific emission signal at 665 nm. The \u003c\/span\u003e\u003cspan\u003eIcotrokinra\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e inhibitor\u003c\/span\u003e\u003cspan\u003e blocks the binding of \u003c\/span\u003e\u003cspan\u003eIL23\u003c\/span\u003e\u003cspan\u003e to \u003c\/span\u003e\u003cspan\u003eIL23R\u003c\/span\u003e\u003cspan\u003e, preventing FRET signal generation. The stronger the blocking effect of the screened drug, the lower the signal. This specific signal is proportional to the extent of the \u003c\/span\u003e\u003cspan\u003eIL23\u003c\/span\u003e\u003cspan\u003e-\u003c\/span\u003e\u003cspan\u003eIL23R\u003c\/span\u003e\u003cspan\u003e interaction. The homogeneous assay is simple to perform and requires no washing steps.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/6d97dada-f6cd-4f68-851f-ab551da84fdc.png\" style=\"margin-left:30px; width:355px; height:auto; vertical-align:middle;\" alt=\"\"\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003ctable border=\"1\" style=\"border-collapse:collapse;width:auto;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eComponent\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eConcentration\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10000T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eStorage Temperature\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag1-23 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag2- IL23R protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAnti-Tag1 Eu antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e25μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e125μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e500μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAnti-Tag2 Ac antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e25×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e500μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2000μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e40mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan\u003eCaution: After the first thaw, immediately aliquot and store at the recommended temperature. Avoid storing diluted samples or subjecting them to repeated freeze-thaw cycles.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003e1. Reagent Preparation\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003e1.1 Thaw all reagents to room temperature before use (equilibrate at room temperature for at least 30 min). The reaction volume for a 384-well low-profile plate is 20 μL (reagent volumes per reaction are shown in the table). Calculate the required volume before preparation and prepare as needed; the following preparation is for reference only, using 500 tests as an example.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003eTable 1. Reagent Preparation\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:1px solid #000000;width:auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003eReagent Name\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003ePreparation\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003eVolume per Well (μL)\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:justify;\"\u003e\u003cspan style=\"color:#000000;\"\u003eAdd 2 mL of 10× Detection buffer to 18 mL of deionized water to dilute to 1×, mix well, and set aside.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e-\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eTag1-23 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eTake 20 μL of Tag1-23 protein stock solution, dilute to 2 mL with 1× Detection buffer, mix well, and set aside.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eTag2- IL23R protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:justify;\"\u003e\u003cspan style=\"color:#000000;\"\u003eTake 20 μL of Tag2-IL23R protein stock solution, dilute to 2 mL with 1× Detection buffer, mix well, and set aside.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eAntibody Mix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\n\u003cp style=\"text-align:justify;\"\u003e\u003cspan style=\"color:#000000;\"\u003eTake 25 μL of Anti-Tag1 Eu antibody stock solution, add 2.475 mL of 1× Detection buffer, mix well, and set aside; take 100 μL of Anti-Tag2 Ac antibody stock solution, add 2.4 mL of 1× Detection buffer, mix well, and set aside;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify;\"\u003e\u003cspan style=\"color:#000000;\"\u003eAfter dilution, mix the Anti-Tag1 Eu antibody and Anti-Tag2 Ac antibody solutions at a 1:1 ratio and set aside.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e10\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eIcotrokinra\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eAccording to the reaction system, dilute the compound with 1× Detection buffer to 10 times the final concentration in the system. For example, if the final concentration is 10 nM, the dilution concentration should be 100 nM.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eSimultaneously, use DMSO diluted with 1× Detection buffer as the negative control well.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003e1.2 Serial Dilution of Test Samples\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003eTaking \u003ci\u003eIcotrokinra\u003c\/i\u003e as an example, the diluent is 1× Detection buffer. To minimize matrix effect interference, it is recommended to use a solution with the same matrix as the test sample for dilution; and the test sample should be adjusted according to its actual concentration.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003eTable 2. Serial Dilution of Positive Control \u003ci\u003eIcotrokinra\u003c\/i\u003e (adjust according to actual conditions)\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:1px solid #000000;width:auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003eIcotrokinra Final Concentration (nM)\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003eIcotrokinra Preparation Concentration (nM)\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003ePreparation Method\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e①\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e40.00\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e400.00\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e1\u003c\/i\u003e μL\u003ci\u003e 20\u003c\/i\u003e μM\u003ci\u003e Icotrokinra +49\u003c\/i\u003e μL\u003ci\u003e \u003c\/i\u003e1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e②\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e26.67\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e266.67\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e30\u003c\/i\u003e μL\u003ci\u003e \u003c\/i\u003e①\u003ci\u003e +25\u003c\/i\u003e μL\u003ci\u003e \u003c\/i\u003e1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e③\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e17.78\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e177.78\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e30\u003c\/i\u003e μL\u003ci\u003e \u003c\/i\u003e②\u003ci\u003e +15\u003c\/i\u003e μL\u003ci\u003e \u003c\/i\u003e1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e④\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e11.85\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e118.52\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e30\u003c\/i\u003e μL\u003ci\u003e \u003c\/i\u003e③\u003ci\u003e +15\u003c\/i\u003e μL\u003ci\u003e \u003c\/i\u003e1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e⑤\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e7.90\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e79.01\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e30\u003c\/i\u003e μL\u003ci\u003e \u003c\/i\u003e④\u003ci\u003e +15\u003c\/i\u003e μL\u003ci\u003e \u003c\/i\u003e1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e⑥\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e5.27\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e52.67\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e30\u003c\/i\u003e μL\u003ci\u003e \u003c\/i\u003e⑤\u003ci\u003e +15\u003c\/i\u003e μL\u003ci\u003e \u003c\/i\u003e1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e⑦\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e3.51\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e35.12\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e30\u003c\/i\u003e μL\u003ci\u003e \u003c\/i\u003e⑥\u003ci\u003e +15\u003c\/i\u003e μL\u003ci\u003e \u003c\/i\u003e1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e⑧\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e2.34\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e23.41\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e30\u003c\/i\u003e μL\u003ci\u003e \u003c\/i\u003e⑦\u003ci\u003e +15\u003c\/i\u003e μL\u003ci\u003e \u003c\/i\u003e1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eBlank\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e0.00\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e0.00\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e30\u003c\/i\u003e μL\u003ci\u003e \u003c\/i\u003e1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003e2. Sample Addition and Controls\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003e2.1 Order of addition for experimental wells: Add 2 μL of test sample, 4 μL of Tag2-IL23R protein working solution, \u003cstrong\u003e4 μL\u003c\/strong\u003e of Tag1-23 \u003cstrong\u003eprotein working solution,\u003c\/strong\u003e and 10 μL of mixed Mix sequentially into the 384-well low-profile plate;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003e2.2 Maximum control: 2 μL of 1× Detection buffer diluent, 4 μL of Tag2-IL23R protein working solution, \u003cstrong\u003e4 μL\u003c\/strong\u003e of Tag1-23 \u003cstrong\u003eprotein working solution,\u003c\/strong\u003e and 10 μL of mixed Mix;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003e2.3 Quality control well (NC): 10 μL of 1× Detection buffer plus 10 μL of Mix.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003eNote: It is recommended to incubate the inhibitor with the inhibited component first. Since Icotrokinra is an inhibitor of IL23R, Icotrokinra and IL23R are incubated first in this experiment.\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:1px solid #000000;width:auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eMaximum Control\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eSample\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eNC\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" rowspan=\"3\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eStep 1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e1× Detection Buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eGradient diluted test sample\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" rowspan=\"4\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e10uL 1× Detection Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"2\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e4μL Tag2-IL23R protein working solution\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"2\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eIncubate for 10 min\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" rowspan=\"2\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eStep 2\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"2\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e4μL Tag1-23\u003cstrong\u003e protein\u003c\/strong\u003e working solution\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"3\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e10μL \u003cstrong\u003eAntibody Mix\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eStep 3\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"3\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eSeal with plate seal \u003cstrong\u003e,\u003c\/strong\u003e incubate at room temperature for 2 h\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"margin-right:8px;\"\u003e3.\u003c\/span\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003eDetection\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003eDetect using a TR-FRET-compatible microplate reader. Excitation wavelength is 320\/340 nm, and emission wavelengths are detected at 620 nm and 665 nm.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#1655A5;\"\u003e\u003cstrong\u003e【Result Calculation】\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003e1) Calculate the signal value (Ratio): Divide the 665 nm fluorescence signal by the 620 nm fluorescence signal and multiply by 10,000.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003eRatio = (665\/620) ×10000\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003e2) Calculate Net signal based on the signal value:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003eNet signal = (Std-NC)\/NC×100\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003e3) Calculate CV (%):\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003eCV (%) = Standard Deviation\/Mean Ratio × 100%\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify;\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#1655A5;\"\u003e\u003cstrong\u003e【Data Example】\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003eThe following data cannot replace data obtained from experiments and is provided only as an example. Results may vary depending on the plate reader.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"image_resized\" style=\"height:auto;margin-left:30px;vertical-align:middle;width:328px;\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/05274cf7-99bf-421f-8893-9d098cbfad16.png\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eNote: Recommended microplate (384-well plate, white, low-profile)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"100T","offer_id":42887927857227,"sku":"UA086129-100T","price":730.0,"currency_code":"USD","in_stock":true},{"title":"500T","offer_id":42887927889995,"sku":"UA086129-500T","price":2180.0,"currency_code":"USD","in_stock":true},{"title":"2500T","offer_id":42887927922763,"sku":"UA086129-2500T","price":8175.0,"currency_code":"USD","in_stock":true},{"title":"10000T","offer_id":42887927955531,"sku":"UA086129-10000T","price":21800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_fcb39384-ce8a-4a00-8457-5b32e0790013.png?v=1788534040"},{"product_id":"unione-tr-fret-human-krasg12dcypa-protac-binding-kit-ua086153","title":"UniOne® TR-FRET Human KRAS[G12D]\/CypA Ternary Binding Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003eThe kit is based on homogeneous time-resolved fluorescence technology (TR-FRET) technology\u003c\/span\u003e\u003cspan\u003e，\u003c\/span\u003e\u003cspan\u003ewhich is used to assess the molecular-mediated interaction between the KRAS[G12D] and \u003c\/span\u003e\u003cspan\u003eCypA\u003c\/span\u003e\u003cspan\u003e. This method provides a high-throughput approach to detect the small molecules capable of mediating the interaction between the KRAS[G12D] and \u003c\/span\u003e\u003cspan\u003eCypA\u003c\/span\u003e\u003cspan\u003e simply and rapidly.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAs shown in the figure below, the interaction between KRAS[G12D] and \u003c\/span\u003e\u003cspan\u003eCypA\u003c\/span\u003e\u003cspan\u003e was detected by an Eu-labeled anti-Tag1 antibody (TR-FRET donor) and an Ac-labeled anti-Tag2 antibody (TR-FRET receptor). Since the molecular adhesive \u003c\/span\u003e\u003cspan\u003eDaraxonrasib\u003c\/span\u003e\u003cspan\u003e mediates the interaction between KRAS[G12D] and \u003c\/span\u003e\u003cspan\u003eCypA\u003c\/span\u003e\u003cspan\u003e, the donor antibody is in close proximity to the receptor antibody. Excitation of the donor antibody triggers fluorescence resonance energy transfer (FRET) to the receptor antibody, resulting in specific emission of a signal at a wavelength of 665 nm from the receptor antibody. This \u003c\/span\u003e\u003cspan\u003especific signal is proportional to the degree of interaction between \u003c\/span\u003e\u003cspan\u003eDaraxonrasib\u003c\/span\u003e\u003cspan\u003e, KRAS[G12D] and \u003c\/span\u003e\u003cspan\u003eCypA\u003c\/span\u003e\u003cspan\u003e. This homogeneous assay is simple to perform and requires no washing steps.\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/19762506-313d-43fd-baf2-cfca2f4b67b5.png\" style=\"margin-left:30px; width:453px; height:auto; vertical-align:middle;\" alt=\"\"\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e【Composition and Storage Conditions】\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse:collapse;width:auto;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eComposition \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003econcentration\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e100T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e2500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e10000T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eStorage \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag1-KRAS[G12D] protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag2-\u003c\/span\u003e\u003cspan\u003eC\u003c\/span\u003e\u003cspan\u003eyp\u003c\/span\u003e\u003cspan\u003eA\u003c\/span\u003e\u003cspan\u003e protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDaraxonrasib\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e400 μM\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e200μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAnti-Tag1 Eu antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e250μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1000μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAnti-Tag2 Ac antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e25μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e125μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e500μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e40mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan\u003eNote: Aliquot into appropriate volumes, store at the recommended temperature and avoid repeated freeze-thaw cycle.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"color:#000000\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"100T","offer_id":42935117545547,"sku":"UA086153-100T","price":730.0,"currency_code":"USD","in_stock":true},{"title":"500T","offer_id":42935117578315,"sku":"UA086153-500T","price":2180.0,"currency_code":"USD","in_stock":true},{"title":"2500T","offer_id":42935117611083,"sku":"UA086153-2500T","price":8175.0,"currency_code":"USD","in_stock":true},{"title":"10000T","offer_id":42935117643851,"sku":"UA086153-10000T","price":21800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_94809046-3674-498f-bf9f-f0c38f7fa12d.png?v=1788537668"},{"product_id":"unione-tr-fret-human-krasg12ccypa-protac-binding-kit-ua086152","title":"UniOne® TR-FRET Human KRAS[G12C]\/CypA Ternary Binding Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eThe kit is based on homogeneous time-resolved fluorescence technology (TR-FRET) technology，which is used to assess the molecular-mediated interaction between the KRAS[G12C] and \u003c\/span\u003e\u003cspan\u003eCypA\u003c\/span\u003e\u003cspan\u003e. This method provides a high-throughput approach to detect the small molecules capable of mediating the interaction between the KRAS[G12C] and \u003c\/span\u003e\u003cspan\u003eCypA\u003c\/span\u003e\u003cspan\u003e simply and rapidly.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eAs shown in the figure below, the interaction between KRAS[G12C] and \u003c\/span\u003e\u003cspan\u003eCypA\u003c\/span\u003e\u003cspan\u003e was detected by an Eu-labeled anti-Tag1 antibody (TR-FRET donor) and an Ac-labeled anti-Tag2 antibody (TR-FRET receptor). Since the molecular adhesive \u003c\/span\u003e\u003cspan\u003eDaraxonrasib\u003c\/span\u003e\u003cspan\u003e mediates the interaction between KRAS[G12C] and \u003c\/span\u003e\u003cspan\u003eCypA\u003c\/span\u003e\u003cspan\u003e, the donor antibody is in close proximity to the receptor antibody. Excitation of the donor \u003c\/span\u003e\u003cspan\u003eantibody triggers fluorescence resonance energy transfer (FRET) to the receptor antibody, resulting in specific emission of a signal at a wavelength of 665 nm from the receptor antibody. This specific signal is proportional to the degree of interaction between \u003c\/span\u003e\u003cspan\u003eDaraxonrasib\u003c\/span\u003e\u003cspan\u003e, KRAS[G12C] and \u003c\/span\u003e\u003cspan\u003eCypA\u003c\/span\u003e\u003cspan\u003e. This homogeneous assay is simple to perform and requires no washing steps.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/9552aea7-8784-4a4b-9724-afaf8249713e.png\" style=\"margin-left:30px; width:463px; height:auto; vertical-align:middle;\" alt=\"\"\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e【Composition and Storage Conditions】\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse:collapse;width:auto;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eComposition \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003econcentration\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e100T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e2500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e10000T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eStorage \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag1-KRAS[G12C] protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag2-\u003c\/span\u003e\u003cspan\u003eC\u003c\/span\u003e\u003cspan\u003eyp\u003c\/span\u003e\u003cspan\u003eA\u003c\/span\u003e\u003cspan\u003e protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDaraxonrasib\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e400 μM\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e200μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAnti-Tag1 Eu antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e150μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1000μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAnti-Tag2 Ac antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e25μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e125μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e500μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e40mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan\u003eNote: Aliquot into appropriate volumes, store at the recommended temperature and avoid repeated freeze-thaw cycle.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"500T","offer_id":42935117742155,"sku":"UA086152-500T","price":2180.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":42935117709387,"sku":"UA086152-100T","price":730.0,"currency_code":"USD","in_stock":true},{"title":"2500T","offer_id":42935117774923,"sku":"UA086152-2500T","price":8175.0,"currency_code":"USD","in_stock":true},{"title":"10000T","offer_id":42935117807691,"sku":"UA086152-10000T","price":21800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_8d7b3595-e99a-424a-9a46-9163cb2c1997.png?v=1788537669"},{"product_id":"thunder-extreme-phospho-erk12-t202y204-tr-fret-cell-signaling-assay-kit-kit-xtm-erkp","title":"THUNDER™ Extreme Phospho-ERK1\/2 (T202\/Y204) TR-FRET Cell Signaling Assay Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eMouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAntigen\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eERK1\/2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman, Mouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cbr\u003eEu-phospho-XTMERK1\/2\u003cbr\u003eAcc-phospho-XTMERK1\/2\u003cbr\u003eERK1\/2 control lysate\u003cbr\u003e5X Lysis Buffer 1\u003cbr\u003e10X TR-FRET Detection buffer\u003cbr\u003e100X Phosphatase Inhibitor Cocktail\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eSandwich TR-FRET immunoassay for semi-quantitative detection of low levels of Phospho-ERK1\/2 (T202\/Y204) in cell lysates. This high-sensitivity kit is suitable for measuring the inhibition of basal phospho-ERK1\/2 levels. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual. \u003cspan style=\"color: #d00400;\"\u003e\u003cstrong\u003eBEST SELLER\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e","brand":"Bioauxilium","offers":[{"title":"100points","offer_id":42952126038091,"sku":"KIT-XTM-ERKP-100","price":409.0,"currency_code":"USD","in_stock":true},{"title":"500points","offer_id":42952126070859,"sku":"KIT-XTM-ERKP-500","price":1283.0,"currency_code":"USD","in_stock":true},{"title":"2500points","offer_id":42952126103627,"sku":"KIT-XTM-ERKP-2500","price":5441.0,"currency_code":"USD","in_stock":true},{"title":"5000points","offer_id":42952126136395,"sku":"KIT-XTM-ERKP-5000","price":6763.0,"currency_code":"USD","in_stock":true},{"title":"10000points","offer_id":42952126169163,"sku":"KIT-XTM-ERKP-10000","price":8390.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/wp-content_uploads_2019_12_Phospho-600x577-1-600x577_62a43336-a731-4585-9ef1-e72a879d7dab.jpg?v=1788314525"},{"product_id":"thunder-human-ifn-tr-fret-biomarker-assay-kit-kit-ifng-10000","title":"THUNDER™ Human IFNγ TR-FRET Biomarker Assay Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eMouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cbr\u003eEu-anti-IFNγ\u003cbr\u003eAcc-anti-IFNγ\u003cbr\u003e5X Assay Buffer 1\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003eSandwich TR-FRET immunoassay for the quantitative measurement of human IFNγ (IFN-gamma; IFN; IFG; type II or immune interferon) in cell culture supernatants. The kit \u003cu\u003edoes not\u003c\/u\u003e include a recombinant human IFNγ standard, which must be purchased separately from R\u0026amp;D Systems® (catalogue number 285-IF-100). A detailed assay protocol and full assay validation can be found in the Technical Data Sheet. \u003cspan style=\"color: #d00400;\"\u003e\u003cstrong\u003eBEST SELLER\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e \u003cp\u003eKey features and benefits:\u003c\/p\u003e \u003cul\u003e  \u003cli\u003eHomogeneous: no wash, easy and rapid protocol\u003c\/li\u003e  \u003cli\u003eHigh sensitivity (15 µL sample), specificity, robustness, and reproducibility\u003c\/li\u003e  \u003cli\u003eHigh-quality antibody pairs ensure lot-to-lot consistency\u003c\/li\u003e  \u003cli\u003eReliable: rigorous development, validation, and quality control testing\u003c\/li\u003e  \u003cli\u003eCost-effective: 5 flexible formats; larger quantities available upon request\u003c\/li\u003e \u003c\/ul\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e","brand":"Bioauxilium","offers":[{"title":"10000points","offer_id":42957122732107,"sku":"KIT-IFNG-10000","price":6862.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/wp-content_uploads_2021_02_Biomarker-600x577-1.jpg?v=1788310806"},{"product_id":"thunder-human-ifn-tr-fret-biomarker-assay-kit-kit-ifng-500","title":"THUNDER™ Human IFNγ TR-FRET Biomarker Assay Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eMouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cbr\u003eEu-anti-IFNγ\u003cbr\u003eAcc-anti-IFNγ\u003cbr\u003e5X Assay Buffer 1\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003eSandwich TR-FRET immunoassay for the quantitative measurement of human IFNγ (IFN-gamma; IFN; IFG; type II or immune interferon) in cell culture supernatants. 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