{"title":"TPD Assay Kits","description":null,"products":[{"product_id":"human-stat6crbn-protac-binding-kit-ua085003","title":"UniOne® TR-FRET Human STAT6\/CRBN PROTAC Binding Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-80℃\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003eThe kit employs homogeneous time-resolved fluorescence (TR-FRET) technology to measure the interaction between molecular glue AK-1690 and test compounds mediating STAT6 and CRBN. This method enables high-throughput screening of small molecules capable of mediating the interaction between STAT6 and CRBN in a simple and rapid manner.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAs shown, the interaction between STAT6 and CRBN is detected using Eu-labeled anti-Tag1 antibody (TR-FRET donor) and Ac-labeled anti-Tag2 antibody (TR-FRET acceptor). Since molecular glue AK-1690 mediates the interaction between STAT6 and CRBN, the proximity of the donor and acceptor antibodies allows fluorescence resonance energy transfer (FRET) from the donor to the acceptor upon excitation, resulting in a specific emission signal at 665 nm. This signal is proportional to the extent of interaction mediated by AK-1690 between STAT6 and CRBN. The homogeneous assay is simple to perform and requires no washing steps.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/ea03772f-6e06-4254-8d49-8ec8aa6d7d4b.png\" style=\"margin-left:30px; width:393px; height:auto; vertical-align:middle;\" alt=\"\"\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003ctable border=\"1\" style=\"border-collapse:collapse;width:auto;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eComponent\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eConcentration\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"\"\u003e100T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"\"\u003e500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"\"\u003e2500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"\"\u003e10000T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eStorage Temperature\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag1-STAT6 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e40μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e200μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e800μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag2- CRBN protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:极1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e40μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e200μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e800μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAK-1690 (150μM)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #极000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e200μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e极p\u0026gt;\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAnti-Tag1 Eu antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e250μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1000μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAnti-Tag2 Ac antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e12.5×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e40μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e200μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:极center\"\u003e\u003cspan\u003e1000μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4000μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #极000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000极;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e40mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003e.\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003e Reagent Preparation\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003e1\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e.1 \u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eThaw all reagents at room temperature before use (equilibrate at room temperature for at least 30 min). The reaction volume for the 384-well shallow plate is 20\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eμ\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eL (reagent volumes for the reaction system are shown in the table). Calculate the required volume for the experiment before preparation and prepare as needed; the following preparation is for reference only, using 500 assays as an example.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"color:#000000\"\u003eTable 1\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e. \u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eReagent Preparation\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eReagent Name\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003ePreparation\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eVolume per Assay Well (μL)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDetection buffer 1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan\u003eTake\u003c\/span\u003e\u003cspan\u003e2mL 10\u003c\/span\u003e\u003cspan\u003e ×\u003c\/span\u003e\u003cspan\u003e Detection buffer1\u003c\/span\u003e\u003cspan\u003eand add\u003c\/span\u003e\u003cspan\u003e18mL deionized water to dilute\u003c\/span\u003e\u003cspan\u003eto\u003c\/span\u003e\u003cspan\u003e1×. Mix\u003c\/span\u003e\u003cspan\u003ewell and set\u003c\/span\u003e\u003cspan\u003easide\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAK-1690\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp\u003e\u003cspan\u003eAccording to the reaction system, dilute the compound with\u003c\/span\u003e\u003cspan\u003e1×Detection buffer to the desired testing concentration\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eEnsure consistent\u003c\/span\u003e\u003cspan\u003eDMSO concentration across all assay wells\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag1-STAT6 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan\u003eTake\u003c\/span\u003e\u003cspan\u003e40μL Tag1-STAT6 protein stock solution and dilute to 2mL with 1× Detection buffer.\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eMix well and set aside.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag2- CRBN protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan\u003eTake\u003c\/span\u003e\u003cspan\u003e40μL Tag2-CRBN protein stock solution and dilute to 2mL with 1×Detection buffer.\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eMix well and set aside.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAntibody Mix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan\u003eTake\u003c\/span\u003e\u003cspan\u003e50μL Anti-Tag1 Eu antibody \u003c\/span\u003e\u003cspan\u003estock solution and add\u003c\/span\u003e\u003cspan\u003e 1×Detection buffer 2.45mL.\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eMix well;\u003c\/span\u003e\u003cspan\u003etake\u003c\/span\u003e\u003cspan\u003e200μL Anti-Tag2 Ac antibody \u003c\/span\u003e\u003cspan\u003estock solution and add\u003c\/span\u003e\u003cspan\u003e 1× Detection buffer 2.3mL.\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eMix well;\u003c\/span\u003e\u003cspan\u003emix the two solutions at a\u003c\/span\u003e\u003cspan\u003e1:1 ratio to prepare the Antibody Mix.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan style=\"color:#000000\"\u003e1.2 \u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eGradient Dilution of Test Samples\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eUsing AK-1690 as an example, the diluent is 1× Detection buffer. To minimize the impact of matrix effects, it is recommended to dilute with a solution matching the matrix of the test sample; adjust the test sample concentrations according to actual conditions.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"color:#000000\"\u003eTable\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e2. AK-1690\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e Gradient Dilution\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e (Adjust according to actual conditions)\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eFinal Concentration of AK-1690 (nM)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003ePreparation Concentration of AK-1690 (nM)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003ePreparation Method\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e①\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e150\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e1500\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003eμL 150μM \u003c\/span\u003e\u003cspan\u003eAK-1690 \u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e99\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e②\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e100\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e1000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003e0μL \u003c\/span\u003e\u003cspan\u003e①\u003c\/span\u003e\u003cspan\u003e +\u003c\/span\u003e\u003cspan\u003e15\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e③\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e66.67 \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e666.67 \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003e0μL \u003c\/span\u003e\u003cspan\u003e②\u003c\/span\u003e\u003cspan\u003e +\u003c\/span\u003e\u003cspan\u003e15\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e④\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e44.44 \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e444.44 \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003e0μL \u003c\/span\u003e\u003cspan\u003e③\u003c\/span\u003e\u003cspan\u003e +\u003c\/span\u003e\u003cspan\u003e15\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑤\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e29.63 \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e296.30 \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003e0μL \u003c\/span\u003e\u003cspan\u003e④\u003c\/span\u003e\u003cspan\u003e +\u003c\/span\u003e\u003cspan\u003e15\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑥\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e19.75 \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e197.53 \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003e0μL \u003c\/span\u003e\u003cspan\u003e⑤\u003c\/span\u003e\u003cspan\u003e +\u003c\/span\u003e\u003cspan\u003e15\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑦\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e13.17 \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e131.69 \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003e0μL \u003c\/span\u003e\u003cspan\u003e⑥\u003c\/span\u003e\u003cspan\u003e +\u003c\/span\u003e\u003cspan\u003e15\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑧\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e8.78 \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e87.79 \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003e0μL \u003c\/span\u003e\u003cspan\u003e⑦\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e15\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑨\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e5.85 \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e58.53 \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003e0μL \u003c\/span\u003e\u003cspan\u003e⑧\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e15\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eBlank\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003e0μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003e2\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003e.\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003e Sample Addition and Controls\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003e2.1 Test Samples: Add 2μL of gradient-diluted test sample, 4μL of Tag1-STAT6 protein working solution, 4μL of Tag2-CRBN protein working solution, and 10μL of mixed Antibody Mix (Tag2-CRBN protein can be mixed with Antibody Mix at a proportional ratio before addition) sequentially into the 384-well shallow plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003e2.2  Positive Control Standard Curve: 2μL of gradient-diluted AK-1690, 4μL of Tag1-STAT6 protein working solution, 4μL of Tag2-CRBN protein working solution, and 10μL of Antibody Mix.\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse:collapse;width:auto;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003ePositive Control Standard Curve\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eTest Samples\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eBlank Control Wells\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNC\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"2\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eStep 1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eGradient-diluted\u003c\/span\u003e\u003cspan\u003eAK-1690\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eGradient-diluted Test Samples\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"4\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e0\u003c\/span\u003e\u003cspan\u003eμL 1\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003cspan\u003e Detection buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 10μL Antibody Mix\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4μL Tag1- STAT6 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eStep 2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4μL Tag2-CRBN protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10μL Antibody Mix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"4\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eSeal the plate wells with a sealing film\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e.\u003c\/span\u003e\u003cspan\u003e Incubate at room temperature for 2 hours;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan style=\"color:#000000\"\u003e2.3 Blank Control Wells: Replace test samples with 2μL of 1× Detection buffer;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003e2.4 NC: 10μL of 1× Detection buffer plus 10μL of Antibody Mi\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003ex\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003eAfter adding all samples, centrifuge, seal with a sealing film, and incubate at room temperature for 2 hours.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e3.\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003e Detection\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003eDetect using a microplate reader compatible with TR-FRET\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e.\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e Excitation wavelength is 320\/340nm, and emission wavelengths are 620nm and 665nm.\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e[Result Calculation]\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003e1)\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e Calculate the signal value\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e (Ratio)\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e: Divide the 665nm fluorescence signal by the 620nm fluorescence signal, then multiply by 10000\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"color:#000000\"\u003eRatio\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e \u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e=\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e \u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e(665\/620)\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e ×\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e10000\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003e2)\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e Calculate\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eNet signal\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e based on the signal value: \u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"color:#000000\"\u003eNet signal\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e = (Std-\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eNC\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e)\/\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eNC\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e×100\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003e3)\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e Calculate CV（%）\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"color:#000000\"\u003eCV（%）= Standard Deviation\/Mean Ratio × 100%\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e[Data Example]\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003eThe following data cannot replace data obtained from actual experiments and is provided for illustration only. Results may vary depending on the plate reader.\u003c\/span\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/91c17fac-7963-4ecc-b512-2ee55dff5bc2.jpeg\" style=\"margin-left:30px; width:464px; height:auto; vertical-align:middle;\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003eNote: Recommended microplate (384-well plate, white, shallow well)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"100T","offer_id":43088829481035,"sku":"UA085003-100T","price":730.0,"currency_code":"USD","in_stock":true},{"title":"500T","offer_id":41887881855051,"sku":"UA085003-500T","price":2180.0,"currency_code":"USD","in_stock":true},{"title":"2500T","offer_id":41887881887819,"sku":"UA085003-2500T","price":8175.0,"currency_code":"USD","in_stock":true},{"title":"10000T","offer_id":41887881920587,"sku":"UA085003-10000T","price":21800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_03e22ed7-830b-4077-babd-5a66e8a20011.png?v=1793379860"},{"product_id":"unione-tr-fret-human-ddb1-crbnirak4-protac-binding-kit-ua086005","title":"UniOne® TR-FRET Human RAK4\/CRBN PROTAC Binding KIT","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-80℃\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-size:11.0pt;color:black\"\u003eThis kit utilizes Time-Resolved Fluorescence ResonanceEnergy Transfer (TR-FRET) technology to measure the interaction between IRAK4and CRBN mediated by the molecular glue PROTAC IRAK4 DEGRADER-1 and testcompounds. This method provides a simple, rapid, and high-throughput format fordetecting small molecules capable of mediating IRAK4-CRBN interactions.\u003c\/span\u003e\u003cspan style=\"font-size:11.0pt\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:11.0pt;color:black\"\u003eAs shown, the interaction between IRAK4 and CRBN isdetected using an anti-Tag1 antibody labeled with Eu (TR-FRET donor) and ananti-Tag2 antibody labeled with Ac (TR-FRET acceptor). Since the molecular gluePROTAC IRAK4 DEGRADER-1 mediates the interaction between IRAK4 and CRBN, thedonor and acceptor antibodies are brought into proximity. Excitation of thedonor antibody triggers fluorescence resonance energy transfer (FRET) to theacceptor antibody, resulting in specific emission of a 665 nm signal. Thisspecific signal is proportional to the degree of interaction between PROTACIRAK4 DEGRADER-1, IRAK4, and CRBN. This homogeneous assay is simple to performand requires no wash steps.\u003c\/span\u003e\u003cspan style=\"font-size:11.0pt\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg 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\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lang=\"EN-US\"\u003e\u003cstrong\u003e500 TESTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:11.0pt;font-weight:normal;' lang=\"EN-US\"\u003e\u003cstrong\u003e2500 TESTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:11.0pt;font-weight:normal;' lang=\"EN-US\"\u003e\u003cstrong\u003e10000 TESTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:11.0pt;font-weight:normal;' lang=\"EN-US\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003eTag1-IRAK4 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e50×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e8 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e40 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e200 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e800 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003eTag2-DDB1\/CRBN protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e50×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e8 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e40 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e200 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e800 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003ePROTAC IRAK4 degrader-1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e3 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e5 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e25 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e125 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003eAnti-Tag1 Eu dye antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e50×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e10 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e50 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e250 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e1000 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003eAnti-Tag2 Ac antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e12.5×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e40 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e200 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e1000 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e5000 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e10×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e1 mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e2 mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e6 mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e25 mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\u003c\/figure\u003e\u003cp style=\"background-color:white;margin-bottom:8.0pt;text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"background-color:white;color:black;\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e*Avoid repeated freeze-thaw cycles of reagents. Recommend aliquoting after first thaw and storing according to conditions specified for a shelf life of one year.\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan style=\"background-color:white;color:black;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:11.0pt;font-weight:normal;' lang=\"EN-US\"\u003e\u003cstrong\u003e1. Reagent Preparation\u003c\/strong\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"background-color:white;margin-bottom:8.0pt;text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"background-color:white;color:black;\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003eThaw all reagents at room temperature (equilibrate at room temperature for at least 30 min) before use. The reaction system for a 384-well shallow plate is 20 μL (reagent volumes per well shown in table). Calculate the required volume based on your experimental needs and prepare accordingly. The following preparation is for reference only, using the 500-test kit as an example.\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"background-color:white;margin-bottom:8.0pt;text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"background-color:white;color:black;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:11.0pt;font-weight:normal;' lang=\"EN-US\"\u003e\u003cstrong\u003e1.1 Buffer (1×):\u003c\/strong\u003e\u003c\/span\u003e\u003c\/span\u003e\u003cbr\u003e\u003cspan style=\"background-color:white;color:black;\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003eAdd \u003c\/span\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:11.0pt;font-weight:normal;' lang=\"EN-US\"\u003e\u003cstrong\u003e18 mL\u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e sterile water to \u003c\/span\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:11.0pt;font-weight:normal;' lang=\"EN-US\"\u003e\u003cstrong\u003e2 mL\u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e Detection buffer (10×) and mix well.\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"background-color:white;margin-bottom:8.0pt;text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"background-color:white;color:black;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:11.0pt;font-weight:normal;' lang=\"EN-US\"\u003e\u003cstrong\u003eTable 2. Reagent Preparation and Volumes\u003c\/strong\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:none;\" border=\"1\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003cthead\u003e\u003ctr\u003e\n\u003cth style=\"border:solid windowtext 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:11.0pt;font-weight:normal;' lang=\"EN-US\"\u003e\u003cstrong\u003eReagent Name\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:11.0pt;font-weight:normal;' lang=\"EN-US\"\u003e\u003cstrong\u003ePreparation\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:11.0pt;font-weight:normal;' lang=\"EN-US\"\u003e\u003cstrong\u003eVolume per well (μL)\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003ePROTAC IRAK4 DEGRADER-1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003eDilute compound to test concentration using 1× Detection buffer according to the reaction system.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003eTag1-IRAK4 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003eTake 40 μL Tag1-IRAK4 protein stock, dilute to 2 mL with 1× Detection buffer, mix well and set aside.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003eTag2-DDB1\/CRBN protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003eTake 40 μL Tag2-DDB1\/CRBN protein stock, dilute to 2 mL with 1× Detection buffer, mix well and set aside.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003eAntibody Mix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003eTake 50 μL Anti-Tag1 Eu dye antibody stock, add 1× Detection buffer to 2.5 mL, mix well; take 200 μL Anti-Tag2 Ac antibody stock, add 1× Detection buffer to 2.5 mL, mix well. Combine 1:1 to make Antibody Mix.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e10\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\u003c\/figure\u003e\u003cp style=\"background-color:white;margin-bottom:8.0pt;text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"background-color:white;color:black;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:11.0pt;font-weight:normal;' lang=\"EN-US\"\u003e\u003cstrong\u003e1.2 Dilution of Test Samples\u003c\/strong\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"background-color:white;margin-bottom:8.0pt;text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"background-color:white;color:black;\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003eUsing PROTAC IRAK4 DEGRADER-1 as an example, diluent is 1× Detection buffer. To minimize matrix interference, it is recommended to dilute with the same matrix as the test sample; adjust based on actual sample concentration.\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"background-color:white;margin-bottom:8.0pt;text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"background-color:white;color:black;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:11.0pt;font-weight:normal;' lang=\"EN-US\"\u003e\u003cstrong\u003eTable 3. Sample Dilution Series\u003c\/strong\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:none;\" border=\"1\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003cthead\u003e\u003ctr\u003e\n\u003cth style=\"border:solid windowtext 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e \u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:11.0pt;font-weight:normal;' lang=\"EN-US\"\u003e\u003cstrong\u003eFinal PROTAC IRAK4 DEGRADER-1 Concentration (nM)\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:11.0pt;font-weight:normal;' lang=\"EN-US\"\u003e\u003cstrong\u003ePrepared PROTAC IRAK4 DEGRADER-1 Concentration (nM)\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:11.0pt;font-weight:normal;' lang=\"EN-US\"\u003e\u003cstrong\u003ePreparation Method\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e150\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e1500\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e2 μL of 150 μM stock + 198 μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e75\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e750\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e50 μL of ① + 50 μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e3\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e37.5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e375\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e50 μL of ② + 50 μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e18.75\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e187.5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e50 μL of ③ + 50 μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e9.38\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e93.75\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e50 μL of ④ + 50 μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e6\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e4.69\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e46.88\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e50 μL of ⑤ + 50 μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e7\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e2.34\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e23.44\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e50 μL of ⑥ + 50 μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e8\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e1.17\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e11.72\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e50 μL of ⑦ + 50 μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e9\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e0.59\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e5.86\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e50 μL of ⑧ + 50 μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003eBlank\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e100 μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\u003c\/figure\u003e\u003cp style=\"background-color:white;margin-bottom:8.0pt;text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"background-color:white;color:black;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:11.0pt;font-weight:normal;' lang=\"EN-US\"\u003e\u003cstrong\u003e2. Sample and Control Setup\u003c\/strong\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"background-color:white;margin-bottom:8.0pt;text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"background-color:white;color:black;\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e2.1 Pipetting order for assay wells: Add 4 μL Tag1-IRAK4 protein working solution, 2 μL test sample, then add 4 μL Tag2-DDB1\/CRBN protein working solution, and 10 μL mixed Antibody Mix sequentially into a 384-well shallow plate.\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"background-color:white;margin-bottom:8.0pt;text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"background-color:white;color:black;\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e2.2 Minimum Control Wells: Use 2 μL 1× Detection buffer in place of the test sample.\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"background-color:white;margin-bottom:8.0pt;text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"background-color:white;color:black;\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e2.3 Maximum Control Wells: Use 2 μL 40000 nM PROTAC IRAK4 DEGRADER-1 in place of the test sample.\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"background-color:white;margin-bottom:8.0pt;text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"background-color:white;color:black;\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e2.4 NC (Eu only): Add 16 μL 1× Detection buffer + 4 μL diluted Anti-Tag1 Eu dye antibody.\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"background-color:white;margin-bottom:8.0pt;text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"background-color:white;color:black;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:11.0pt;font-weight:normal;' lang=\"EN-US\"\u003e\u003cstrong\u003eTable 4. Plate Layout and Controls\u003c\/strong\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:none;\" border=\"1\" cellspacing=\"0\" cellpadding=\"0\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:59.6pt;\" width=\"79\" valign=\"top\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:98.5pt;\" width=\"131\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003eMinimum Control\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:97.45pt;\" width=\"130\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003eMaximum Control\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:93.45pt;\" width=\"125\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003eSample\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:76.95pt;\" width=\"103\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003eNC\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:59.6pt;\" rowspan=\"2\" width=\"79\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003eStep 1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:98.5pt;\" width=\"131\" valign=\"top\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:97.45pt;\" width=\"130\" valign=\"top\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e1500nM \u003c\/span\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003ePROTAC IRAK4 DEGRADER-1\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:93.45pt;\" width=\"125\" valign=\"top\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003eSerial dilution of test sample\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:76.95pt;\" rowspan=\"4\" width=\"103\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e15μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003eadd 5μL 1×Anti-Tag1 Eu dye antibody\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:31.7pt;\"\u003e\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:31.7pt;padding:0cm 5.4pt 0cm 5.4pt;width:289.4pt;\" colspan=\"3\" width=\"386\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e4μL Tag1-IRAK4 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:59.6pt;\" rowspan=\"3\" width=\"79\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003eStep 2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:289.4pt;\" colspan=\"3\" width=\"386\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e4μL Tag2-DDB1\/CRBN protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:289.4pt;\" colspan=\"3\" width=\"386\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e10μL Antibody Mix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:366.35pt;\" colspan=\"4\" width=\"488\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003eSeal plate with plate sealer and incubate at room temperature for 2 hours\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp style=\"background-color:white;margin-bottom:8.0pt;text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"background-color:white;color:black;\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e*For negative controls and NC, it is recommended to use matrix-matched solution instead of 1× Detection buffer.\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"background-color:white;margin-bottom:8.0pt;text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"background-color:white;color:black;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:11.0pt;font-weight:normal;' lang=\"EN-US\"\u003e\u003cstrong\u003e3. Detection\u003c\/strong\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"background-color:white;margin-bottom:8.0pt;text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"background-color:white;color:black;\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003eRead on a TR-FRET-compatible plate reader (excitation 320 nm, emission detection at 620 nm and 665 nm).\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"background-color:white;margin-bottom:8.0pt;text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"background-color:white;color:black;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:11.0pt;font-weight:normal;' lang=\"EN-US\"\u003e\u003cstrong\u003e[Data Analysis]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"background-color:white;text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"background-color:white;color:black;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:11.0pt;font-weight:normal;' lang=\"EN-US\"\u003e\u003cstrong\u003eCalculate Signal Value:\u003c\/strong\u003e\u003c\/span\u003e\u003c\/span\u003e\u003cbr\u003e\u003cspan style=\"background-color:white;color:black;\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003eSignal Value = (665 nm fluorescence \/ 620 nm fluorescence) × 10,000\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"margin-bottom:6.0pt;vertical-align:baseline;\"\u003e \u003c\/p\u003e\n\u003cp style=\"background-color:white;text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"background-color:white;color:black;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:11.0pt;font-weight:normal;' lang=\"EN-US\"\u003e\u003cstrong\u003eCalculate Net Signal:\u003c\/strong\u003e\u003c\/span\u003e\u003c\/span\u003e\u003cbr\u003e\u003cspan style=\"background-color:white;color:black;\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003eNet Signal = (Std – NC) \/ NC × 100\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"vertical-align:baseline;\"\u003e \u003c\/p\u003e\n\u003cp style=\"background-color:white;text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"background-color:white;color:black;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:11.0pt;font-weight:normal;' lang=\"EN-US\"\u003e\u003cstrong\u003eCalculate CV (%):\u003c\/strong\u003e\u003c\/span\u003e\u003c\/span\u003e\u003cbr\u003e\u003cspan style=\"background-color:white;color:black;\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003eCV (%) = (Standard Deviation \/ Mean Ratio) × 100%\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"margin-left:-18.0pt;vertical-align:baseline;\"\u003e \u003c\/p\u003e\n\u003cp style=\"background-color:white;margin-bottom:8.0pt;text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"background-color:white;color:black;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;font-size:11.0pt;font-weight:normal;' lang=\"EN-US\"\u003e\u003cstrong\u003e[Example Data]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"background-color:white;margin-bottom:8.0pt;text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"background-color:white;color:black;\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003eThe following data are for illustrative purposes only and cannot replace data obtained from your experiment. Results may vary depending on the TR-FRET-compatible instrument used.\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:none;\" border=\"1\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003cthead\u003e\u003ctr\u003e\n\u003cth style=\"border:solid windowtext 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003ePROTAC IRAK4 degrader-1 (nM)\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003eAverage Ratio\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003eCV\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003eAverage Net Signal\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e125\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e231.3\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e5.0%\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e621.35\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e62.5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e225.4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e5.7%\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e602.95\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e31.25\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e169.4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e2.7%\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e428.30\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e15.625\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e102.335\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e5.2%\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e219.15\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e7.8125\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e63.97\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e0.4%\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e99.50\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e3.90625\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e50.34\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e11.3%\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e56.99\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e0 (DMSO)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e38.455\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e4.1%\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e19.93\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003eEu + AC\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e32.155\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e2.9%\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e0.28\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003eS\/B\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e6.01\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003eIC₅₀\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:5.0pt 5.0pt 5.0pt 5.0pt;\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:baseline;\" align=\"left\"\u003e\u003cspan style=\"font-size:11.0pt;\" lang=\"EN-US\"\u003e23.96\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e \u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"500T","offer_id":42332756836427,"sku":"UA086005-500T","price":2180.0,"currency_code":"USD","in_stock":true},{"title":"2500T","offer_id":42332756869195,"sku":"UA086005-2500T","price":8175.0,"currency_code":"USD","in_stock":true},{"title":"10000T","offer_id":42332756901963,"sku":"UA086005-10000T","price":21800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/37f11b0e40d04782925eef0914f69818.png?v=1793422861"},{"product_id":"unione-tr-fret-human-ddb1-crbngspt1-protac-binding-kit-ua086006","title":"UniOne® TR-FRET Human GSPT1\/CRBN PROTAC Binding Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003eThe kit employs homogeneous time-resolved fluorescence technology (\u003c\/span\u003e\u003cspan\u003eTR-FRET\u003c\/span\u003e\u003cspan\u003e) to measure the interaction between the molecular glue\u003c\/span\u003e\u003cspan\u003eCC-885\u003c\/span\u003e\u003cspan\u003eand the compound under test, mediated by the\u003c\/span\u003e\u003cspan\u003eDDB1\/CRBN\u003c\/span\u003e\u003cspan\u003ecomplex and\u003c\/span\u003e\u003cspan\u003eGSPT1.\u003c\/span\u003e\u003cspan\u003eThis method provides a simple and rapid high-throughput assay for identifying small molecules capable of mediating the interaction between the\u003c\/span\u003e\u003cspan\u003eDDB1\/CRBN\u003c\/span\u003e\u003cspan\u003ecomplex and\u003c\/span\u003e\u003cspan\u003eGSPT1.\u003c\/span\u003e\u003cspan\u003eAs shown in the figure,\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003ethe interaction between\u003c\/span\u003e\u003cspan\u003eDDB1\/CRBN\u003c\/span\u003e\u003cspan\u003eand\u003c\/span\u003e\u003cspan\u003eGSPT1\u003c\/span\u003e\u003cspan\u003eis detected using an\u003c\/span\u003e\u003cspan\u003eEu\u003c\/span\u003e\u003cspan\u003elabeled anti-\u003c\/span\u003e\u003cspan\u003eTag1\u003c\/span\u003e\u003cspan\u003eantibody (\u003c\/span\u003e\u003cspan\u003eTR-FRET\u003c\/span\u003e\u003cspan\u003edonor) and an\u003c\/span\u003e\u003cspan\u003eaccepter\u003c\/span\u003e\u003cspan\u003elabeled anti-\u003c\/span\u003e\u003cspan\u003eTag2\u003c\/span\u003e\u003cspan\u003eantibody (\u003c\/span\u003e\u003cspan\u003eTR-FRET\u003c\/span\u003e\u003cspan\u003eacceptor). Because the molecular glue\u003c\/span\u003e\u003cspan\u003eCC-885\u003c\/span\u003e\u003cspan\u003emediates the interaction between\u003c\/span\u003e\u003cspan\u003eDDB1\/CRBN\u003c\/span\u003e\u003cspan\u003eand\u003c\/span\u003e\u003cspan\u003eGSPT1,\u003c\/span\u003e\u003cspan\u003ethe donor antibody and the acceptor antibody come into close proximity, and excitation of the donor antibody triggers fluorescence resonance energy transfer (\u003c\/span\u003e\u003cspan\u003eFRET\u003c\/span\u003e\u003cspan\u003e) to the acceptor antibody, causing the acceptor antibody to emit fluorescence specifically at\u003c\/span\u003e\u003cspan\u003e665 nm.\u003c\/span\u003e\u003cspan\u003eA signal with a specific wavelength. This particular signal is directly proportional to the extent of interaction between\u003c\/span\u003e\u003cspan\u003eCC-885\u003c\/span\u003e\u003cspan\u003eand\u003c\/span\u003e\u003cspan\u003eDDB1\/CRBN\u003c\/span\u003e\u003cspan\u003e,\u003c\/span\u003e\u003cspan\u003eGSPT1\u003c\/span\u003e\u003cspan\u003e. This homogeneous assay is easy to perform and requires no washing steps.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/f01ccbcb-026a-44a3-b1f1-ddd5c955c484.png\" style=\"margin-left:30px; width:441px; height:auto; vertical-align:middle;\"\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003ctable border=\"1\" style=\"border-collapse:collapse;width:auto;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eComponent\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eConcentration\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e100T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e2500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e10000T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eStorage Temperature\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag1-DDB1\/CRBN protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003e0×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e400\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag2-GSPT1 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003e0×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCC-885\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4mM\u003c\/span\u003e\u003cspan\u003ein\u003c\/span\u003e\u003cspan\u003eDMSO\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e25\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAnti-Tag1 Eu antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003e0\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e250\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003e00\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAnti-Tag2 Ac antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e125\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e500\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e0\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e400μ\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003emL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003emL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e40\u003c\/span\u003e\u003cspan\u003emL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan\u003eNote: After the first thaw, aliquot immediately and store at the recommended temperature. Avoid storing after dilution and repeated freeze-thaw cycles.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e,\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eReagent preparation\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e.1\u003c\/span\u003e\u003cspan\u003eBefore use, allow all reagents to thaw at room temperature (equilibrate at room temperature for at least 30 minutes). The reaction volume in a 384-well plate is 20.\u003c\/span\u003e\u003cspan\u003emu\u003c\/span\u003e\u003cspan\u003eL (the reagent volumes for the reaction system are shown in the table). Calculate the required volume before preparation and prepare accordingly. The following formulation is for reference only, using 500 reactions as an example.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eTable 1\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003cspan\u003eReagent preparation and usage amounts\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eReagent name\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eConfiguration\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eμL per test well\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003etake\u003c\/span\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003em\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003cspan\u003eDetection buffer stock solution,\u003c\/span\u003e\u003cspan\u003eAdd to 18 ml ddH₂O.\u003c\/span\u003e\u003csub\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/sub\u003e\u003cspan\u003eIn O, mix thoroughly to obtain\u003c\/span\u003e\u003cspan\u003e1× Detection buffer\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eStandard substance\u003c\/span\u003e\u003cspan\u003e\/\u003c\/span\u003e\u003cspan\u003eSample to be tested\u003c\/span\u003e\u003cspan\u003eDilution\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eUse diluted to\u003c\/span\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003etimes\u003c\/span\u003e\u003cspan\u003eof\u003c\/span\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003cspan\u003ePrepare the samples to be tested and the standard solutions. If the stock solution of the sample is…\u003c\/span\u003e\u003cspan\u003eDMSO\u003c\/span\u003e\u003cspan\u003e, it is recommended to maintain the system.\u003c\/span\u003e\u003cspan\u003eDMSO\u003c\/span\u003e\u003cspan\u003eThe content is consistent and does not exceed the maximum limit.\u003c\/span\u003e\u003cspan\u003e2%\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003cspan\u003eStandard dilutions are shown in the table below.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag1-DDB1\/CRBN protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003etake\u003c\/span\u003e\u003cspan\u003e20 μL Tag1-DDB1\/CRBN protein\u003c\/span\u003e\u003cspan\u003eUndiluted solution,\u003c\/span\u003e\u003cspan\u003e1× Detection buffer\u003c\/span\u003e\u003cspan\u003eDilute to\u003c\/span\u003e\u003cspan\u003e2 mL,\u003c\/span\u003e\u003cspan\u003eMix well and set aside.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag2-GSPT1 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003etake\u003c\/span\u003e\u003cspan\u003e20 μL Tag2-GSPT1 protein\u003c\/span\u003e\u003cspan\u003eUndiluted solution,\u003c\/span\u003e\u003cspan\u003eDilute 1× detection buffer to a final volume of 2 mL.\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eMix well and set aside.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAntibody\u003c\/span\u003e\u003cspan\u003eMix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003etake\u003c\/span\u003e\u003cspan\u003eFifty\u003c\/span\u003e\u003cspan\u003eμL Anti-Tag1 Eu antibody\u003c\/span\u003e\u003cspan\u003eConcentrate, add\u003c\/span\u003e\u003cspan\u003e1× Detection buffer 2.45 mL,\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e \u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eSufficient\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eMix well\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e;\u003c\/span\u003e\u003cspan\u003etake\u003c\/span\u003e\u003cspan\u003e25 μL Anti-Tag2 Ac antibody\u003c\/span\u003e\u003cspan\u003eConcentrate, add\u003c\/span\u003e\u003cspan\u003e1× Detection buffer 2.4\u003c\/span\u003e\u003cspan\u003eseventy-five\u003c\/span\u003e\u003cspan\u003emL,\u003c\/span\u003e\u003cspan\u003eMix thoroughly; combine the above two components in a 1:1 ratio to obtain the Antibody Mix.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan\u003e1.2\u003c\/span\u003e\u003cspan\u003eStandard Preparation Table\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003ePlease prepare the total amount of standard solutions based on the experimental conditions; the following information is for reference only.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eUse diluted to\u003c\/span\u003e\u003cspan\u003e1X\u003c\/span\u003e\u003cspan\u003ePrepare the samples to be tested and the standards using the assay buffer. If the stock solution of the sample is…\u003c\/span\u003e\u003cspan\u003eDMSO\u003c\/span\u003e\u003cspan\u003e, it is recommended to maintain within the system.\u003c\/span\u003e\u003cspan\u003eDMSO\u003c\/span\u003e\u003cspan\u003eConsistent content,\u003c\/span\u003e\u003cspan\u003e(\u003c\/span\u003e\u003cspan\u003eThe detection buffer does not contain\u003c\/span\u003e\u003cspan\u003eDMSO\u003c\/span\u003e\u003cspan\u003ePlease, based on the dilution of the standard or the sample to be tested, proceed to…\u003c\/span\u003e\u003cspan\u003e1X\u003c\/span\u003e\u003cspan\u003eAdded to the detection buffer\u003c\/span\u003e\u003cspan\u003eDMSO)\u003c\/span\u003e\u003cspan\u003eand not exceeding a maximum of\u003c\/span\u003e\u003cspan\u003e2%\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eTable\u003c\/span\u003e\u003cspan\u003e2.\u003c\/span\u003e\u003cspan\u003eStandard Preparation Table\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eCC-885\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eFinal concentration (\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003enM\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eCC-885\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003ePreparation concentration (nM)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eConfiguration method\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4000.00\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e40000.0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1 μL 4 mM\u003c\/span\u003e\u003cspan\u003eMother liquor\u003c\/span\u003e\u003cspan\u003e+99 μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1333.33\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e13333.33\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e30 μL\u003c\/span\u003e\u003cspan\u003e①\u003c\/span\u003e\u003cspan\u003e+60 μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e3\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e444.44\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4444.44\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e30 μL\u003c\/span\u003e\u003cspan\u003e②\u003c\/span\u003e\u003cspan\u003e+60 μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e148.15\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1481.48\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e30 μL\u003c\/span\u003e\u003cspan\u003e③\u003c\/span\u003e\u003cspan\u003e+60 μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e49.38\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e493.83\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e30 μL\u003c\/span\u003e\u003cspan\u003e④\u003c\/span\u003e\u003cspan\u003e+60 μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e6\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e16.46\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e164.61\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e30 μL\u003c\/span\u003e\u003cspan\u003e⑤\u003c\/span\u003e\u003cspan\u003e+60 μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e7\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5.49\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e54.87\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e30 μL\u003c\/span\u003e\u003cspan\u003e⑥\u003c\/span\u003e\u003cspan\u003e+60 μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e8\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1.83\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e18.29\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e30 μL\u003c\/span\u003e\u003cspan\u003e⑥\u003c\/span\u003e\u003cspan\u003e+60 μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e9\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e0.61\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e6.1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e30 μL\u003c\/span\u003e\u003cspan\u003e⑥\u003c\/span\u003e\u003cspan\u003e+60 μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eBlank\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e60 μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e2.\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eSample addition and controls\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.1 Sample to be tested: Add 2 μL of the gradient-diluted sample, 4 μL of Tag1-DDB1\/CRBN protein working solution, and 4 μL of Tag2-GSPT1 protein into a 384-well plate in sequence.\u003c\/span\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003cspan\u003eAntibody\u003c\/span\u003e\u003cspan\u003eMix\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.2 Positive control standard curve: Add 2 μL of CC-885 diluted in a gradient, 4 μL of Tag1-DDB1\/CRBN protein working solution, 4 μL of Tag2-GSPT1 protein,\u003c\/span\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003cspan\u003eAntibody\u003c\/span\u003e\u003cspan\u003eMix\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e.3\u003c\/span\u003e\u003cspan\u003eNegative control well (NC): Replace the sample to be tested with 2 μL of 1× Detection buffer;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.4 Eu reference well: Add 10 μL of 1× Detection buffer plus\u003c\/span\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003cspan\u003eAntibody\u003c\/span\u003e\u003cspan\u003eMix\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse:collapse;width:auto;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003ePositive control standard curve\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eSample to be tested\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNegative control well (NC)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eEu reference well\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"4\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eStep one\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eGradient dilution\u003c\/span\u003e\u003cspan\u003eCC-885\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eGradient dilute the sample to be tested\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"5\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10μL 1\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e10μL Antibody Mix\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eTag1-DDB1\/CRBN protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4μL Tag2- GSPT1 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eSeal the plate,\u003c\/span\u003e\u003cspan\u003e1000rpm\u003c\/span\u003e\u003cspan\u003e, centrifuge\u003c\/span\u003e\u003cspan\u003e1min\u003c\/span\u003e\u003cspan\u003eMix well,\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eincubate at room temperature\u003c\/span\u003e\u003cspan\u003e10min\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"2\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eStep two\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10μL Antibody Mix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"4\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eSeal the plate,\u003c\/span\u003e\u003cspan\u003e1000rpm\u003c\/span\u003e\u003cspan\u003e, centrifuge\u003c\/span\u003e\u003cspan\u003e1min\u003c\/span\u003e\u003cspan\u003eMix well,\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eincubate at room temperature\u003c\/span\u003e\u003cspan\u003e120min\u003c\/span\u003e\u003cspan\u003e, read the plate\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e3,\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003edetection\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eDetect on a TR-FRET-compatible microplate reader\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003cspan\u003eExcitation light is 320\/340nm, detecting emission wavelengths at 620nm and 665nm.\u003c\/span\u003e\u003cspan\u003eEmission\u003c\/span\u003e\u003cspan\u003ewavelengths.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e[Result Calculation]\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1)\u003c\/span\u003e\u003cspan\u003eCalculate the signal value\u003c\/span\u003e\u003cspan\u003e(Ratio)\u003c\/span\u003e\u003cspan\u003e: The 665nm fluorescence signal is multiplied by 10,000 compared to the 620nm fluorescence signal.\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eRatio\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e=\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e(665\/620)\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003cspan\u003e10000\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2)\u003c\/span\u003e\u003cspan\u003eCalculate based on the signal value.\u003c\/span\u003e\u003cspan\u003eNet signal\u003c\/span\u003e\u003cspan\u003e:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eNet signal\u003c\/span\u003e\u003cspan\u003e= (Std-Blank)\/Blank × 100\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3)\u003c\/span\u003e\u003cspan\u003eCalculate CV (%).\u003c\/span\u003e\u003cspan\u003e:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eCV (%) = Standard Deviation \/ Mean Ratio × 100%\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e[Data Example]\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe following data cannot replace the data obtained in experiments; it is only for illustrative purposes, and the results may\u003c\/span\u003e\u003cspan\u003evary depending on the plate reader.\u003c\/span\u003e\u003cspan\u003eInstrument\u003c\/span\u003e\u003cspan\u003emay vary.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e(1) Standard Curve*\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe following data cannot replace the data obtained in experiments; it is only for illustrative purposes, and the results may vary depending on\u003c\/span\u003e\u003cspan\u003eTR-FRET\u003c\/span\u003e\u003cspan\u003ecompatible instruments.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#FFFFFF\"\u003e \u003c\/span\u003e\u003cspan style=\"color:#FFFFFF\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/5ca31b5e-ff97-4d7e-96de-6d62acab7f50.png\" style=\"margin-left:30px; width:480px; height:auto; vertical-align:middle;\"\u003e\u003c\/p\u003e\n\u003cspan style=\"color:#FFFFFF\"\u003e \u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e*\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eData are provided for illustrative reference only; a microplate is recommended\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e(384-well plate, white, shallow wells).\u003c\/span\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"100T","offer_id":43184007708747,"sku":"UA086006-100T","price":730.0,"currency_code":"USD","in_stock":true},{"title":"500T","offer_id":42332756934731,"sku":"UA086006-500T","price":2180.0,"currency_code":"USD","in_stock":true},{"title":"2500T","offer_id":42332756967499,"sku":"UA086006-2500T","price":8175.0,"currency_code":"USD","in_stock":true},{"title":"10000T","offer_id":42332757000267,"sku":"UA086006-10000T","price":21800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_a877105b-87a4-42ec-8683-1c68d2e464d6.png?v=1793422865"},{"product_id":"unione-tr-fret-human-brd4crbn-protac-binding-kit-ua086009","title":"UniOne® TR-FRET Human BRD4\/CRBN PROTAC Binding Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-80℃\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-size:11.0pt;color:#0F1115\"\u003eThis kit utilizes Homogeneous Time-Resolved Fluorescence(TR-FRET) technology to measure the Molecular Glue BET Degrader-1 and testcompound-mediated interaction between the DDB1\/CRBN complex and BRD4. BRD4contains bromodomain 1 and 2. This method enables a simple and rapidhigh-throughput format for detecting small molecules capable of mediating theinteraction between the DDB1\/CRBN complex and BRD4.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:11.0pt;color:#0F1115\"\u003eAs shown, the interaction between DDB1\/CRBN and BRD4 isdetected using a Eu-labeled Anti-Tag1 Antibody (TR-FRET donor) and anAcceptor-labeled Anti-Tag2 Antibody (TR-FRET acceptor). As the Molecular GlueBET Degrader-1 mediates the interaction between DDB1\/CRBN and BRD4, the donorand acceptor antibodies are brought into proximity. Excitation of the donor antibodytriggers Fluorescence Resonance Energy Transfer (FRET) to the acceptorantibody, resulting in a specific emission signal at 665nm. This specificsignal is proportional to the degree of interaction mediated by BET Degrader-1between DDB1\/CRBN and BRD4. This homogeneous assay is simple to perform andrequires no washing steps.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/product.template\/20289075\/bce87b3c-36fe-4f6f-a172-3262c154d777.png?access_token=ccc20f25-7f3b-4ebe-8eb2-1a373a3d129d\" class=\"img img-fluid o_we_custom_image\" style=\"width: 50%;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:none;\" border=\"1\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003cthead\u003e\u003ctr\u003e\n\u003cth style=\"border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e500 TESTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e2500 TESTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e10000 TESTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eTag1-DDB1\/CRBN protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e25×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e80μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1600μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eTag2-BRD4 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e20μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e100μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eBET Degrader-1 (100μM)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10μM\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e200μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eAnti-Tag1 Eu dye antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e250μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1000μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eAnti-Tag2 Ac antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e12.5×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e200μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1000μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e4000μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e40mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\u003c\/figure\u003e\u003cp style=\"background-color:white;margin:12.0pt 0cm;text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:#0F1115;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e*Repeated freeze-thaw cycles should be avoided. Aliquot upon first thaw and store according to the specified conditions. Valid for one year.\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e1\u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003eReagent Preparation\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;text-autospace:none;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eThaw all reagents to room temperature before use (equilibrate at room temperature for at least\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e30min\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e).\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e384\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e-well shallow plate reaction volume is\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e20uL\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e (reagent volumes for the reaction system are shown in the table). Calculate the required volume for the experiment before preparation and prepare accordingly; the following preparation is for reference only, using\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e500\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e assays as an example.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;text-autospace:none;\" align=\"left\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e1.1 \u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003ePreparation of Buffer (\u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e1\u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003e×)\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"Default\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e2mL\u003c\/strong\u003e Detection buffer \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e(\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e×)\u003c\/span\u003e\u003cspan style=\"color:windowtext;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003e, add\u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e18mL\u003c\/strong\u003e\u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003esterile water, mix well, and set aside.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"Default\"\u003e \u003c\/p\u003e\n\u003cp style=\"background-color:white;margin-right:9.0pt;text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eTable\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2. \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eReagent Preparation and Volumes\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:none;margin-left:10.15pt;\" border=\"1\" cellspacing=\"0\" cellpadding=\"0\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:114.2pt;\" width=\"152\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003eReagent Name\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:232.85pt;\" width=\"310\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003ePreparation\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:61.7pt;\" width=\"82\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003eVolume (\u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003eμL\u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003e)\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:114.2pt;\" width=\"152\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eBET Degrader-1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:232.85pt;\" width=\"310\" valign=\"top\"\u003e\n\u003cp\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eAccording to the reaction system, dilute the compound to the desired concentration using\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e×\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eEnsure consistent\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDMSO\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003econcentration across all assay wells.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:61.7pt;\" width=\"82\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:114.2pt;\" width=\"152\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eTag1-DDB1\/CRBN protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:232.85pt;\" width=\"310\" valign=\"top\"\u003e\n\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eTake\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e80μL Tag1-DDB1\/CRBN protein\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003estock solution,\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e× \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003edilute to\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2mL ,\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003emix well, and set aside.\u003c\/strong\u003e\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:61.7pt;\" width=\"82\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:114.2pt;\" width=\"152\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eTag2- BRD4 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:232.85pt;\" width=\"310\" valign=\"top\"\u003e\n\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eTake\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e20μL Tag2- BRD4 protein\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003estock solution,\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e×\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003edilute to\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2mL ,\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003emix well, and set aside.\u003c\/strong\u003e\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:61.7pt;\" width=\"82\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:114.2pt;\" width=\"152\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eAnti-Tag1 Eu dye antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:232.85pt;\" width=\"310\" valign=\"top\"\u003e\n\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eTake\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50μL Anti-Tag1 Eu dye antibody \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003estock solution, add \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e×\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer 2.45mL,\u003cstrong\u003e \u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003emix well, and set aside.\u003c\/strong\u003e\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:61.7pt;\" width=\"82\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:114.2pt;\" width=\"152\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eAnti-Tag2 Ac antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:232.85pt;\" width=\"310\" valign=\"top\"\u003e\n\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eTake\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e200μL Anti-Tag2 Ac antibody \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003estock solution, add \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e× \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer 2.3mL,\u003cstrong\u003e \u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003emix well, and set aside.\u003c\/strong\u003e\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:61.7pt;\" width=\"82\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp class=\"Default\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e1.2 Mix-1\u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003ePreparation\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;text-autospace:none;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eAnti-Tag1 Eu dye antibody\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eand\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eAnti-Tag2 Ac antibody\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eafter diluting separately,\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1:1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003emix well, and set aside.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e1.3 \u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eSample\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e-Fold Serial Dilution\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;text-indent:21.0pt;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eUsing\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eBET Degrader-1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eas an example, the diluent is\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e× \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e. To minimize matrix effect interference, it is recommended to dilute with a solution matching the matrix of the test sample; adjust the test sample concentration according to actual conditions.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eTable\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e3. \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eSerial Dilution of Samples\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;margin-left:6.75pt;margin-right:6.75pt;\" border=\"0\" cellspacing=\"0\" cellpadding=\"0\" align=\"left\" width=\"95%\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height:25.15pt;\"\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;height:25.15pt;padding:0cm 5.4pt 0cm 5.4pt;width:9.28%;\" width=\"9%\" nowrap valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid windowtext 1.0pt;height:25.15pt;padding:0cm 5.4pt 0cm 5.4pt;width:20.64%;\" width=\"20%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eBET Degrader-1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eFinal Concentration (\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003enM\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid windowtext 1.0pt;height:25.15pt;padding:0cm 5.4pt 0cm 5.4pt;width:26.72%;\" width=\"26%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eSample or Standard\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid windowtext 1.0pt;height:25.15pt;padding:0cm 5.4pt 0cm 5.4pt;width:43.32%;\" width=\"43%\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eSolution\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:25.15pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:25.15pt;padding:0cm 5.4pt 0cm 5.4pt;width:9.28%;\" width=\"9%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003ePositive Control\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:25.15pt;padding:0cm 5.4pt 0cm 5.4pt;width:20.64%;\" width=\"20%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:25.15pt;padding:0cm 5.4pt 0cm 5.4pt;width:26.72%;\" width=\"26%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1 μL BET Degrader-1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e(\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e100 μM\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:25.15pt;padding:0cm 5.4pt 0cm 5.4pt;width:43.32%;\" width=\"43%\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e99 \u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eμL 1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e× \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:17.95pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:17.95pt;padding:0cm 5.4pt 0cm 5.4pt;width:9.28%;\" width=\"9%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e①\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:17.95pt;padding:0cm 5.4pt 0cm 5.4pt;width:20.64%;\" width=\"20%\" nowrap\u003e\u003cp style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e100.00\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:17.95pt;padding:0cm 5.4pt 0cm 5.4pt;width:26.72%;\" width=\"26%\" nowrap valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e100\u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eμL\u003c\/span\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e 1\u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eμ\u003c\/span\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eM \u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eBET Degrader-1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:17.95pt;padding:0cm 5.4pt 0cm 5.4pt;width:43.32%;\" width=\"43%\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e0\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:16.55pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:16.55pt;padding:0cm 5.4pt 0cm 5.4pt;width:9.28%;\" width=\"9%\" nowrap valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e②\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:16.55pt;padding:0cm 5.4pt 0cm 5.4pt;width:20.64%;\" width=\"20%\" nowrap\u003e\u003cp style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50.00\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:16.55pt;padding:0cm 5.4pt 0cm 5.4pt;width:26.72%;\" width=\"26%\" nowrap valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50\u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eμL\u003c\/span\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e \u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e①\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:16.55pt;padding:0cm 5.4pt 0cm 5.4pt;width:43.32%;\" width=\"43%\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50\u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eμL\u003c\/span\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e \u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e× \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e(\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1%DMSO\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:17.6pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:17.6pt;padding:0cm 5.4pt 0cm 5.4pt;width:9.28%;\" width=\"9%\" nowrap valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e③\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:17.6pt;padding:0cm 5.4pt 0cm 5.4pt;width:20.64%;\" width=\"20%\" nowrap\u003e\u003cp style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e25.00\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:17.6pt;padding:0cm 5.4pt 0cm 5.4pt;width:26.72%;\" width=\"26%\" nowrap valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50\u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eμL\u003c\/span\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e \u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e②\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:17.6pt;padding:0cm 5.4pt 0cm 5.4pt;width:43.32%;\" width=\"43%\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50\u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eμL\u003c\/span\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e \u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e× \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e(\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1%DMSO\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:16.25pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:16.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:9.28%;\" width=\"9%\" nowrap valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e④\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:16.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:20.64%;\" width=\"20%\" nowrap\u003e\u003cp style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e12.50\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:16.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:26.72%;\" width=\"26%\" nowrap valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50\u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eμL\u003c\/span\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e \u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e③\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:16.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:43.32%;\" width=\"43%\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50\u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eμL\u003c\/span\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e \u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e× \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e(\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1%DMSO\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:14.85pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:14.85pt;padding:0cm 5.4pt 0cm 5.4pt;width:9.28%;\" width=\"9%\" nowrap valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e⑤\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:14.85pt;padding:0cm 5.4pt 0cm 5.4pt;width:20.64%;\" width=\"20%\" nowrap\u003e\u003cp style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e6.25\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:14.85pt;padding:0cm 5.4pt 0cm 5.4pt;width:26.72%;\" width=\"26%\" nowrap valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50\u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eμL\u003c\/span\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e \u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e④\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:14.85pt;padding:0cm 5.4pt 0cm 5.4pt;width:43.32%;\" width=\"43%\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50\u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eμL\u003c\/span\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e \u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e× \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e(\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1%DMSO\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:4.5pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:4.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:9.28%;\" width=\"9%\" nowrap valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e⑥\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:4.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:20.64%;\" width=\"20%\" nowrap\u003e\u003cp style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e3.13\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:4.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:26.72%;\" width=\"26%\" nowrap valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50\u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eμL\u003c\/span\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e \u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e⑤\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:4.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:43.32%;\" width=\"43%\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50\u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eμL\u003c\/span\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e \u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e× \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e(\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1%DMSO\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:18.0pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:18.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:9.28%;\" width=\"9%\" nowrap valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e⑦\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:18.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:20.64%;\" width=\"20%\" nowrap\u003e\u003cp style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1.56\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:18.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:26.72%;\" width=\"26%\" nowrap valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50\u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eμL\u003c\/span\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e \u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e⑥\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:18.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:43.32%;\" width=\"43%\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50\u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eμL\u003c\/span\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e \u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e× \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e(\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1%DMSO\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:16.9pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:16.9pt;padding:0cm 5.4pt 0cm 5.4pt;width:9.28%;\" width=\"9%\" nowrap valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e⑧\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:16.9pt;padding:0cm 5.4pt 0cm 5.4pt;width:20.64%;\" width=\"20%\" nowrap\u003e\u003cp style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e0.78\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:16.9pt;padding:0cm 5.4pt 0cm 5.4pt;width:26.72%;\" width=\"26%\" nowrap valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50\u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eμL\u003c\/span\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e \u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e⑦\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:16.9pt;padding:0cm 5.4pt 0cm 5.4pt;width:43.32%;\" width=\"43%\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50\u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eμL\u003c\/span\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e \u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e× \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e(\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1%DMSO\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:17.25pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:17.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:9.28%;\" width=\"9%\" nowrap valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e⑨\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:17.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:20.64%;\" width=\"20%\" nowrap\u003e\u003cp style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e0.39\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:17.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:26.72%;\" width=\"26%\" nowrap valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50\u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eμL\u003c\/span\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e \u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e⑧\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:17.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:43.32%;\" width=\"43%\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50\u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eμL\u003c\/span\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e \u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e× \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e(\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1%DMSO\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:17.25pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:17.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:9.28%;\" width=\"9%\" nowrap valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e⑩\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:17.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:20.64%;\" width=\"20%\" nowrap\u003e\u003cp style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e0.20\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:17.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:26.72%;\" width=\"26%\" nowrap valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50\u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eμL\u003c\/span\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e⑨\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:17.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:43.32%;\" width=\"43%\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50\u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eμL\u003c\/span\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e \u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e× \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e(\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1%DMSO\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:16.8pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:16.8pt;padding:0cm 5.4pt 0cm 5.4pt;width:9.28%;\" width=\"9%\" nowrap valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eBlank\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:16.8pt;padding:0cm 5.4pt 0cm 5.4pt;width:20.64%;\" width=\"20%\" nowrap valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:16.8pt;padding:0cm 5.4pt 0cm 5.4pt;width:26.72%;\" width=\"26%\" nowrap valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:16.8pt;padding:0cm 5.4pt 0cm 5.4pt;width:43.32%;\" width=\"43%\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e100\u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eμL\u003c\/span\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e \u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e× \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e(\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1%DMSO\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp style=\"clear:both;\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e2. \u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003eSample Addition and Controls\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;text-autospace:none;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2.1 \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eAddition order for assay wells:\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e4uL Tag1-DDB1\/CRBN protein\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e,\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e4μL Tag2- BRD4 protein\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e,\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2μL BET Degrader-1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eor test sample,\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10μL\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eof mixed\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eMix-1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e, add sequentially into the\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e384\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e-well shallow plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;text-autospace:none;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2.2 \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eNegative control wells (\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eBlank\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e):\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e BET Degrader-1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eor test compound is replaced with\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2μL\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eof\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDMSO\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003esolution at the same percentage.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2.3 NC\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e:\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e16uL 1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e× buffer plus\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e4μL\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eof diluted\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eAnti-Tag1 Eu dye antibody\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eTable\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e4. \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eSample Addition and Controls\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:none;\" border=\"1\" cellspacing=\"0\" cellpadding=\"0\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:59.6pt;\" width=\"79\" valign=\"top\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:98.5pt;\" width=\"131\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eNegative Control\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:97.45pt;\" width=\"130\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003ePositive Control\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:93.45pt;\" width=\"125\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eSample\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:76.95pt;\" width=\"103\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eNC\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:59.6pt;\" rowspan=\"4\" width=\"79\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eStep 1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:98.5pt;\" width=\"131\" valign=\"top\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e× \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:97.45pt;\" width=\"130\" valign=\"top\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e100nM BET Degrader-1\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:93.45pt;\" width=\"125\" valign=\"top\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eSerially diluted test sample\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:76.95pt;\" rowspan=\"5\" width=\"103\" valign=\"top\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e15uL 1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e× \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eplus\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e5uL 1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e×\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eAnti-Tag1 Eu dye antibody\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:289.4pt;\" colspan=\"3\" width=\"386\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e4μL Tag1-DDB1\/CRBN protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:289.4pt;\" colspan=\"3\" width=\"386\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e4μL Tag2- BRD4 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:289.4pt;\" colspan=\"3\" width=\"386\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eIncubate at room temperature for\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10 min\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:59.6pt;\" rowspan=\"2\" width=\"79\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eStep 2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:289.4pt;\" colspan=\"3\" width=\"386\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10μL Mix-1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:366.35pt;\" colspan=\"4\" width=\"488\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eSeal the plate with an adhesive seal\u003cstrong\u003e,\u003c\/strong\u003e incubate at room temperature for\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2h\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e*\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eFor negative controls and quality control, it is recommended to replace\u003c\/span\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e1\u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e×\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003ewith a solution matching the matrix of the test sample.\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e3.\u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003eDetection\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;text-autospace:none;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eDetect using a\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eTR-FRET\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e-compatible microplate reader (excitation wavelength:\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e320nm\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e; emission wavelengths:\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e620nm\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eand\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e665nm\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;text-autospace:none;\" align=\"left\"\u003e \u003c\/p\u003e\n\u003cp style=\"background-color:white;line-height:150%;margin:0cm 9.0pt 0cm 0cm;\"\u003e\u003cspan style=\"color:#7030A0;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;line-height:150%;\"\u003e\u003cstrong\u003e[Result Calculation]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"Default\"\u003e\u003cspan style=\"color:windowtext;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1. \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eCalculate signal value:\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e665nm\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003efluorescence signal divided by\u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e620nm\u003c\/span\u003e\u003cspan style=\"color:windowtext;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003efluorescence signal, then multiplied by\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10000\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e, i.e.,\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"Default\"\u003e\u003cspan style=\"color:windowtext;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eSignal Value\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e=\u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e \u003c\/span\u003e\u003cspan style=\"color:windowtext;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e(665\/620)*10000\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"Default\"\u003e\u003cspan style=\"color:windowtext;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2. \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eCalculate the δ value based on the signal value:\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"Default\"\u003e\u003cspan style=\"color:windowtext;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eδ Value\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e= (Std-NC)\/NC*100\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;text-autospace:none;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e3.\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eCalculate\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eCV\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e(\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e%\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eCV\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e(\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e%\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e)\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e= Standard Deviation\/Mean Ratio×100%\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"background-color:white;line-height:150%;margin:0cm 9.0pt 0cm 0cm;\"\u003e\u003cspan style=\"color:#7030A0;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;line-height:150%;\"\u003e\u003cstrong\u003e[Data Example]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eThe following data cannot replace data obtained from actual experiments and is provided for illustration only. Results may vary depending on the\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eTR-FRET\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e-compatible instrument used.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"100T","offer_id":43090353160267,"sku":"UA086009-100T","price":730.0,"currency_code":"USD","in_stock":true},{"title":"500T","offer_id":42332757229643,"sku":"UA086009-500T","price":2180.0,"currency_code":"USD","in_stock":true},{"title":"2500T","offer_id":42332757262411,"sku":"UA086009-2500T","price":8175.0,"currency_code":"USD","in_stock":true},{"title":"10000T","offer_id":42332757295179,"sku":"UA086009-10000T","price":21800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/253c778b82d44bcc8ee19df0a72e50c2.png?v=1793422984"},{"product_id":"unione-tr-fret-human-bcl-xlcrbn-protac-binding-kit-ua086017","title":"UniOne® TR-FRET Human BCL-XL\/CRBN PROTAC Binding Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-80℃\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eThe assay kit employs homogeneous time-resolved fluorescence (TR-FRET) technology to measure the interaction between the DDB1\/CRBN complex and BCL-XL mediated by the molecular glue XZ739 and test compounds. This method enables a simple, rapid, and high-throughput screening of small molecules capable of mediating the interaction between the DDB1\/CRBN complex and BCL-XL.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eAs shown in the figure below, the interaction between DDB1\/CRBN and BCL-XL is detected using a Eu-labeled anti-Tag1 antibody (TR-FRET donor) and an acceptor-labeled anti-Tag2 antibody (TR-FRET acceptor). The molecular glue XZ739 mediates the interaction between DDB1\/CRBN and BCL-XL, bringing the donor and acceptor antibodies into proximity. Excitation of the donor antibody triggers fluorescence resonance energy transfer (FRET) to the acceptor antibody, resulting in a specific emission signal at 665 nm. This signal is proportional to the extent of interaction mediated by XZ739 with DDB1\/CRBN and BCL-XL. The homogeneous assay is simple to perform and requires no washing steps.\u003c\/span\u003e\u003cspan\u003e      \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/a87a39c5-d556-4218-818b-a4632c8ae8d6.png\" style=\"margin-left:30px; width:544px; height:auto; vertical-align:middle;\" alt=\"\"\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003eHere is the translated content in English while preserving the original formatting:```html\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse:collapse;width:auto;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eComponent\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eConcentration\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e100T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e2500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e10000T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eStorage Temperature\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag1-CRBN protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e8\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e40μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e200μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e800μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag2- \u003c\/span\u003e\u003cspan\u003eBCL-XL \u003c\/span\u003e\u003cspan\u003eprotein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eXZ739\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4m\u003c\/span\u003e\u003cspan\u003eM\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e25\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAnti-Tag1 Eu antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003e0×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e25\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e125\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAnti-Tag2 Ac antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e25\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e000μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e40mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan\u003eNote: Aliquot all components immediately after first thawing and store at the recommended temperature. Avoid storage after dilution and repeated freeze-thaw cycles.\u003c\/span\u003e\u003c\/p\u003e```\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003e1. Reagent Preparation\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003e1.1 Before use, thaw all reagents at room temperature (equilibrate at room temperature for at least 30 min). The reaction volume for a 384-well shallow well plate is 20 μL (reagent volumes for the reaction system are shown in the table). Calculate the required volume before preparation and prepare as needed; the following preparation is for reference only, using 500 tests as an example.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003eTable 1. Reagent Preparation\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\" style=\"width:100%;\"\u003e\u003ctable class=\"ck-table-resized\" style=\"border-collapse:collapse;border:1px solid #000000;width:auto;\"\u003e\n\u003ccolgroup\u003e\n\u003ccol style=\"width:9.98%;\"\u003e\n\u003ccol style=\"width:81.14%;\"\u003e\n\u003ccol style=\"width:8.88%;\"\u003e\n\u003c\/colgroup\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003eReagent Name\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003ePreparation\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003eVolume per Detection Well (μL)\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eDetection buffer \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:justify;\"\u003e\u003cspan style=\"color:#000000;\"\u003eAdd 2 mL of 10× Detection buffer 1 to 18 mL of deionized water, dilute to 1×, mix well, and set aside.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e-\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eXZ739\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:justify;\"\u003e\u003cspan style=\"color:#000000;\"\u003eAccording to the reaction system, dilute the compound to the desired concentration using 1× Detection buffer.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eTag1-CRBN protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:justify;\"\u003e\u003cspan style=\"color:#000000;\"\u003eTake 40 μL of Tag1-CRBN protein stock solution, dilute to 2 mL with 1× Detection buffer, \u003cstrong\u003emix well, and set aside.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eTag2- BCL-XL protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:justify;\"\u003e\u003cspan style=\"color:#000000;\"\u003eTake 20 μL of Tag2-BCL-XL protein stock solution, dilute to 2 mL with 1× Detection buffer, \u003cstrong\u003emix well, and set aside.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eAntibody Mix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:justify;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003eTake 25 μL of Anti-Tag1 Eu antibody \u003c\/strong\u003estock solution, add 2.475 mL of 1× Detection buffer, \u003cstrong\u003emix well; take 100 μL of Anti-Tag2 Ac antibody \u003c\/strong\u003estock solution, add 2.4 mL of 1× Detection buffer, \u003cstrong\u003emix well;\u003c\/strong\u003e mix both solutions 1:1 to prepare the Antibody Mix.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e10\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003e1.2 Gradient Dilution of Test Samples\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003eUsing XZ739 as an example, the diluent is 1× Detection buffer. To minimize matrix effect interference, it is recommended to dilute with a solution matching the test sample matrix; adjust the test samples according to actual concentrations.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003eTable 2. XZ739 Gradient Dilution (Adjust According to Actual Conditions)\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:1px solid #000000;width:auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eXZ739 \u003cstrong\u003eFinal Concentration (nM)\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eXZ739 \u003cstrong\u003ePreparation Concentration (nM)\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003ePreparation Method\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e①\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e10000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e100000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e1μL 4mM XZ739 +39μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e②\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e5000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e50000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e20μL ① +20μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e③\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e2500\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e25000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e20μL ② +20μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e④\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e1250\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e12500\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e20μL ③ +20μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑤\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e625\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e6250\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e20μL ④ +20μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑥\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e312.50\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e3125\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e20μL ⑤ +20μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑦\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e156.25\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e1562.50\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e20μL ⑥ +20μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑧\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e78.13\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e781.25\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e20μL ⑦+20μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑨\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e39.06\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e390.63\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e20μL ⑧+20μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑩\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e19.53\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e195.31\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e20μL ⑨+20μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#333333;\"\u003e⑪\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e9.77\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e97.66\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e20μL ⑩+20μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eBlank\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e20μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003e2. Sample Addition and Controls\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003e2.1 Test Samples: Add 4 μL Tag1-CRBN protein working solution, 4 μL Tag2-BCL-XL protein working solution, 2 μL gradient-diluted test samples, and 10 μL mixed Antibody Mix sequentially into the 384-well shallow well plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003e2.2 Positive Control Standard Curve: 4 μL Tag1-CRBN protein working solution, 4 μL Tag2-BCL-XL protein working solution, 2 μL gradient-diluted XZ739, and 10 μL Antibody Mix.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003e2.3 Blank Control Wells: Replace test samples with 2 μL 1× Detection buffer;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003e2.4 NC (Negative Control): Add 10 μL 1× Detection buffer and 10 μL Antibody Mix.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003eAfter adding all samples, centrifuge, seal with a plate sealing film, and incubate at room temperature for 2 hours.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"margin-right:8px;\"\u003e3. \u003c\/span\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003eDetection\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:1px solid #000000;width:auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003ePositive Control Standard Curve\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003eTest Samples\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003eBlank Control Wells\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003eNC\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e2 μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eGradient-diluted XZ739\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e2 μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eGradient-diluted test samples\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e2 μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" rowspan=\"4\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e10 μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eAdd 10 μL Antibody Mix\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"3\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e4 μL Tag1-CRBN protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"3\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e4 μL Tag2-BCL-XL protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"3\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e10 μL Antibody Mix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"4\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003eSeal the plate wells with a sealing film \u003cstrong\u003e,\u003c\/strong\u003e and incubate at room temperature for 2 hours;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003eDetect using a microplate reader compatible with TR-FRET. Excitation wavelength is 320\/340 nm, and emission wavelengths detected are 620 nm and 665 nm.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#1655A5;\"\u003e\u003cstrong\u003e[Result Calculation]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003e1) Calculate signal value (Ratio): Divide the fluorescence signal at 665 nm by the signal at 620 nm, then multiply by 10000.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003eRatio = (665\/620) ×10000\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003e2) Calculate Net signal based on the signal value:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003eNet signal = (Std-NC)\/NC×100\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003e3) Calculate CV (%):\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003eCV（%）= Standard Deviation\/Mean Ratio × 100%\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#1655A5;\"\u003e\u003cstrong\u003e[Data Example]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#000000;\"\u003eThe following data cannot replace experimental results and is provided only as an example; results may vary depending on the plate reader used.   \u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"image_resized\" style=\"height:auto;margin-left:30px;vertical-align:middle;width:465px;\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/e74f52f1-a2fc-45dd-82c0-20a39d9b871d.png\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan style=\"color:#FFFFFF;\"\u003e3 \/3\u003c\/span\u003e\u003cspan style=\"color:#000000;\"\u003e\u003cstrong\u003eNote: Recommended microplates (384-well, white, shallow well);\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"100T","offer_id":42968279023691,"sku":"UA086017-100T","price":730.0,"currency_code":"USD","in_stock":true},{"title":"500T","offer_id":42332758081611,"sku":"UA086017-500T","price":2180.0,"currency_code":"USD","in_stock":true},{"title":"2500T","offer_id":42332758114379,"sku":"UA086017-2500T","price":8175.0,"currency_code":"USD","in_stock":true},{"title":"10000T","offer_id":42332758147147,"sku":"UA086017-10000T","price":21800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_94b1e408-ea4b-45b1-bbe0-114160e92c3d.png?v=1793423032"},{"product_id":"unione-tr-fret-human-bcl-xlvhl-protac-binding-kit-ua086019","title":"UniOne® TR-FRET Human BCL-XL\/VHL PROTAC Binding Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-80℃\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-size:11.0pt\"\u003eThis kit uses HomogeneousTime-Resolved Fluorescence (TR-FRET) technology to measure the molecular glueBCL-XL DEGRADER-2 and test compound-mediated interaction between VHL andBCL-XL. This method enables a simple and rapid high-throughput format for screeningsmall molecules that can mediate the interaction between VHL and BCL-XL.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:11.0pt\"\u003eAs shown, the interaction betweenVHL and BCL-XL is detected using an Eu-labeled anti-Tag1 antibody (TR-FRETdonor) and an Acceptor-labeled anti-Tag2 antibody (TR-FRET acceptor). Since themolecular glue BCL-XL DEGRADER-2 mediates the interaction between VHL andBCL-XL, the donor and acceptor antibodies are brought into proximity.Excitation of the donor antibody triggers Fluorescence Resonance EnergyTransfer (FRET) to the acceptor antibody, resulting in a specific emissionsignal at 665 nm from the acceptor. This specific signal is proportional to thedegree of interaction mediated by BCL-XL DEGRADER-2 between VHL and BCL-XL.This homogeneous assay is simple to perform and requires no washing steps.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/product.template\/20289224\/19013550-84c6-427b-a961-9cbf1af8ff95.png?access_token=cd9d7b6d-f159-4ed9-a40a-32b8fd42f52f\" class=\"img img-fluid o_we_custom_image\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"margin:12.0pt 0cm;text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eTable 1. Reagents and Storage Conditions\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:none;\" border=\"1\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003cthead\u003e\u003ctr\u003e\n\u003cth style=\"border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e100TESTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e500TESTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e2500TESTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e10000TESTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eTag1-VHL protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e40μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e200μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e800μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eTag2- BCL-XL protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e40μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e200μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e800μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eBCL-XL DEGRADER-2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e0.3 mM\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e5μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e20μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e200μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eAnti-Tag1 Eu dye antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e250μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1000μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eAnti-Tag2 AC antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e5μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e25μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e125μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e500μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2 mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10 mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e40 mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80°C\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\u003c\/figure\u003e\u003cp style=\"margin:12.0pt 0cm;text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e*Reagents should be avoided from repeated freeze-thaw cycles. Aliquot upon first thaw and store under recommended conditions. Valid for one year.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e1\u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003eReagent Preparation\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;text-autospace:none;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eBefore use, thaw all reagents at room temperature (equilibrate at room temperature for at least\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e30min\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e).\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e384\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e well plate reaction volume is\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e20uL\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e (reagent volumes for the reaction system are shown in the table); calculate the required volume before preparation and prepare as needed; the following preparation is for reference only, taking\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e500\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e assays as an example.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;text-autospace:none;\" align=\"left\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e1.1 \u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003ePreparation of Buffer (\u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e1\u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003e×\u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003e)\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"Default\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e2mL\u003c\/strong\u003e Detection buffer \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e（\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e×）\u003c\/span\u003e\u003cspan style=\"color:windowtext;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003e, add \u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e18mL\u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003e sterile water, mix well and set aside\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;text-autospace:none;\" align=\"left\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:center;text-autospace:none;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eTable\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2. \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eReagent Preparation and Volumes\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:none;\" border=\"1\" cellspacing=\"0\" cellpadding=\"0\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:145.9pt;\" width=\"195\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003eReagent Name\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:196.85pt;\" width=\"262\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003ePreparation\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:75.6pt;\" width=\"101\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003eVolume (\u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003eμL\u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003e)\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:145.9pt;\" width=\"195\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eTag1-VHL protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:196.85pt;\" width=\"262\" valign=\"top\"\u003e\n\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eTake \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e40μL Tag1-VHL protein\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e stock solution, dilute with \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e× \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e to \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2mL ,\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003emix well and set aside.\u003c\/strong\u003e\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:75.6pt;\" width=\"101\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:145.9pt;\" width=\"195\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eTag2- BCL-XL protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:196.85pt;\" width=\"262\" valign=\"top\"\u003e\n\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eTake \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e40μL Tag2- BCL-XL protein\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e stock solution, dilute with \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e×\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e to \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2mL ,\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003emix well and set aside.\u003c\/strong\u003e\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:75.6pt;\" width=\"101\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:145.9pt;\" width=\"195\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eBCL-XL DEGRADER-2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:196.85pt;\" width=\"262\" valign=\"top\"\u003e\n\u003cp\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eAccording to the reaction system, dilute the compound with \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e×\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e to the desired concentration.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eEnsure consistent \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDMSO\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e concentration in all assay wells.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:75.6pt;\" width=\"101\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:145.9pt;\" width=\"195\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eAntibody Mix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:196.85pt;\" width=\"262\" valign=\"top\"\u003e\n\u003cp\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eTake \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50μL Anti-Tag1 Eu antibody  \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003estock solution, add \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e×\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer 2.45mL,\u003cstrong\u003e \u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003emix well;\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eTake \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e25μL Anti-Tag2 Ac antibody \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003estock solution, add \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e× \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer 2.475mL,\u003cstrong\u003e \u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003emix well,\u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1:1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e mix to form \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eAntibody Mix\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:75.6pt;\" width=\"101\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e1.2 \u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003e2-fold Serial Dilution of Standards for Testing\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;text-indent:21.0pt;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eUsing \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eBCL-XL DEGRADER-2\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e as an example, the diluent is \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e×\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e. \u003c\/span\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eTo minimize matrix effect interference, it is recommended to dilute with a solution matching the sample matrix; adjust tested samples according to actual concentrations.\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eTable\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e3. \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eSample Serial Dilution\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv align=\"center\"\u003e\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;\" border=\"0\" cellspacing=\"0\" cellpadding=\"0\" width=\"97%\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height:27.95pt;\"\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;height:27.95pt;padding:0cm 5.4pt 0cm 5.4pt;width:10.12%;\" width=\"10%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid windowtext 1.0pt;height:27.95pt;padding:0cm 5.4pt 0cm 5.4pt;width:18.8%;\" width=\"18%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eBCL-XL DEGRADER-2\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e Final Concentration (\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003enM\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid windowtext 1.0pt;height:27.95pt;padding:0cm 5.4pt 0cm 5.4pt;width:19.76%;\" width=\"19%\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eBCL-XL DEGRADER-2\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e Preparation Concentration (\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003enM\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid windowtext 1.0pt;height:27.95pt;padding:0cm 5.4pt 0cm 5.4pt;width:51.32%;\" width=\"51%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003ePreparation Method\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:15.25pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:15.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:10.12%;\" width=\"10%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e①\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:15.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:18.8%;\" width=\"18%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e300\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:15.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:19.76%;\" width=\"19%\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e3000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:15.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:51.32%;\" width=\"51%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2μL 0.3mM \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003estock solution\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e+198μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:15.25pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:15.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:10.12%;\" width=\"10%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e②\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:15.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:18.8%;\" width=\"18%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e150\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:15.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:19.76%;\" width=\"19%\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1500\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:15.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:51.32%;\" width=\"51%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50μL \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e①\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e +50μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:15.25pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:15.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:10.12%;\" width=\"10%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e③\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:15.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:18.8%;\" width=\"18%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e75\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:15.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:19.76%;\" width=\"19%\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e750\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:15.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:51.32%;\" width=\"51%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50μL \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e②\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e +50μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:15.25pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:15.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:10.12%;\" width=\"10%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e④\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:15.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:18.8%;\" width=\"18%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e37.5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:15.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:19.76%;\" width=\"19%\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e375\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:15.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:51.32%;\" width=\"51%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50μL \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e③\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e +50μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:15.25pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:15.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:10.12%;\" width=\"10%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e⑤\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:15.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:18.8%;\" width=\"18%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e18.75\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:15.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:19.76%;\" width=\"19%\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e187.5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:15.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:51.32%;\" width=\"51%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50μL \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e④\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e +50μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:15.25pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:15.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:10.12%;\" width=\"10%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e⑥\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:15.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:18.8%;\" width=\"18%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e9.38\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:15.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:19.76%;\" width=\"19%\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e93.75\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:15.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:51.32%;\" width=\"51%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50μL \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e⑤\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e +50μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:15.25pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:15.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:10.12%;\" width=\"10%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e⑦\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:15.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:18.8%;\" width=\"18%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e4.69\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:15.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:19.76%;\" width=\"19%\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e46.88\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:15.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:51.32%;\" width=\"51%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50μL \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e⑥\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e +50μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:15.25pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:15.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:10.12%;\" width=\"10%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e⑧\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:15.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:18.8%;\" width=\"18%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2.34\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:15.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:19.76%;\" width=\"19%\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e23.44\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:15.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:51.32%;\" width=\"51%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50μL \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e⑦\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e +50μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:15.25pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:15.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:10.12%;\" width=\"10%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e⑨\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:15.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:18.8%;\" width=\"18%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1.17\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:15.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:19.76%;\" width=\"19%\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e11.72\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:15.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:51.32%;\" width=\"51%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50μL \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e⑧\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e +50μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:15.25pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:15.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:10.12%;\" width=\"10%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e⑩\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:15.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:18.8%;\" width=\"18%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e0.59\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:15.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:19.76%;\" width=\"19%\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e5.86\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:15.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:51.32%;\" width=\"51%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50μL \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e⑨\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e +50μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:15.25pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:15.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:10.12%;\" width=\"10%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eBlank\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:15.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:18.8%;\" width=\"18%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:15.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:19.76%;\" width=\"19%\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:15.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:51.32%;\" width=\"51%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e100μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003c\/div\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e2 \u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003ePipetting and Controls\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;text-autospace:none;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2.1 \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003ePipetting order for assay wells:\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e4μL Tag1-VHL protein\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e, \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e4μL Tag2- BCL-XL protein\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e, \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2μL BCL-XL DEGRADER-2 \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eor tested samples, \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10μL\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e mixed \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eAntibody Mix\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e, add sequentially to \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e384\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e well plates.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;text-autospace:none;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2.2 \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eNegative control wells (\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eBlank\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e): Replace \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eBCL-XL DEGRADER-2 \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eor tested compounds with \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2uL\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e of \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDMSO\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e solution at the same percentage.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2.3 \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eQuality control wells: Add \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e5uL\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e of diluted \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eAnti-Tag1 Eu dye antibody\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e to \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e15uL Detection buffer\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eTable\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e4. \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003ePipetting and Controls\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:none;\" border=\"1\" cellspacing=\"0\" cellpadding=\"0\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:59.6pt;\" width=\"79\" valign=\"top\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:98.5pt;\" width=\"131\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eNegative Control\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:89.45pt;\" width=\"119\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003ePositive Control\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:101.45pt;\" width=\"135\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eSample\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:76.95pt;\" width=\"103\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eNC\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:59.6pt;\" rowspan=\"3\" width=\"79\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eStep 1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:98.5pt;\" width=\"131\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e× \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e diluted \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDMSO\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:89.45pt;\" width=\"119\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e3μM BCL-XL DEGRADER-2\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:101.45pt;\" width=\"135\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eSerially diluted tested samples\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:76.95pt;\" rowspan=\"4\" width=\"103\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e15μL 1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e× \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eplus \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e5μL 1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e×\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eAnti-Tag1 Eu dye antibody\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:19.6pt;\"\u003e\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:19.6pt;padding:0cm 5.4pt 0cm 5.4pt;width:289.4pt;\" colspan=\"3\" width=\"386\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e4μL Tag1-VHL protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr style=\"height:20.9pt;\"\u003e\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:20.9pt;padding:0cm 5.4pt 0cm 5.4pt;width:289.4pt;\" colspan=\"3\" width=\"386\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e4μL Tag2- BCL-XL protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:59.6pt;\" rowspan=\"2\" width=\"79\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eStep 2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:289.4pt;\" colspan=\"3\" width=\"386\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10μL Antibody Mix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:366.35pt;\" colspan=\"4\" width=\"488\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eSeal plate wells with a sealing film and incubate at room temperature for \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1-2\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e hours;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e*\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eFor negative control and \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eNC\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e, it is recommended to replace \u003c\/span\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003ewith a solution matching the sample matrix instead of \u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e×\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e.\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e3.\u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003eDetection\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;text-autospace:none;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eDetect on a microplate reader compatible with \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eTR-FRET\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e (excitation at \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e320nm\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e, detection of emission wavelengths at \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e620nm\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e and \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e665nm\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;text-autospace:none;\" align=\"left\"\u003e \u003c\/p\u003e\n\u003cp style=\"background-color:white;line-height:150%;margin:0cm 9.0pt 0cm 0cm;\"\u003e\u003cspan style=\"color:#7030A0;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;line-height:150%;\"\u003e\u003cstrong\u003e[Result Calculation]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"Default\"\u003e\u003cspan style=\"color:windowtext;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1. \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eCalculate signal value: Divide the fluorescence signal at \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e665nm\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e by the fluorescence signal at \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e620nm\u003c\/span\u003e\u003cspan style=\"color:windowtext;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e and then multiply by \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10000\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e, i.e.,\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"Default\"\u003e\u003cspan style=\"color:windowtext;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eSignal Value\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e=\u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e \u003c\/span\u003e\u003cspan style=\"color:windowtext;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e(665\/620)*10000\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"Default\"\u003e\u003cspan style=\"color:windowtext;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2. \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eCalculate \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eNet Signal\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e value based on signal value:\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"Default\"\u003e\u003cspan style=\"color:windowtext;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eNet Signal\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e Value\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e= (Std-NC)\/NC*100\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;text-autospace:none;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e3.\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eCalculate \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eCV\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e (\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e%\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eCV\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e (\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e%\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e)\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e= Standard Deviation\/Mean Ratio×100%\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"background-color:white;line-height:150%;margin:0cm 9.0pt 0cm 0cm;\"\u003e\u003cspan style=\"color:#7030A0;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;line-height:150%;\"\u003e\u003cstrong\u003e[Data Example]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eThe following data cannot replace experimental results and is for illustration only; results may vary depending on the \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eTR-FRET\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e compatible instrument used.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e \u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;width:269.05pt;\" border=\"0\" cellspacing=\"0\" cellpadding=\"0\" width=\"359\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height:20.8pt;\"\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;height:20.8pt;padding:0cm 5.4pt 0cm 5.4pt;width:96.55pt;\" width=\"129\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eBCL-XL DEGRADER-2,nM\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid windowtext 1.0pt;height:20.8pt;padding:0cm 5.4pt 0cm 5.4pt;width:57.8pt;\" width=\"77\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eRatio\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid windowtext 1.0pt;height:20.8pt;padding:0cm 5.4pt 0cm 5.4pt;width:57.8pt;\" width=\"77\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eCV\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid windowtext 1.0pt;height:20.8pt;padding:0cm 5.4pt 0cm 5.4pt;width:56.9pt;\" width=\"76\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eNet Signal\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:12.5pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:12.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:96.55pt;\" width=\"129\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e300\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:12.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:57.8pt;\" width=\"77\" nowrap valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e247.9\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:12.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:57.8pt;\" width=\"77\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e3.0%\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:12.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:56.9pt;\" width=\"76\" nowrap valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e881.59\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:12.5pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:12.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:96.55pt;\" width=\"129\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e150\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:12.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:57.8pt;\" width=\"77\" nowrap valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e247.2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:12.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:57.8pt;\" width=\"77\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1.5%\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:12.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:56.9pt;\" width=\"76\" nowrap valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e878.82\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:12.5pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:12.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:96.55pt;\" width=\"129\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e75\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:12.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:57.8pt;\" width=\"77\" nowrap valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e193.6\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:12.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:57.8pt;\" width=\"77\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e6.1%\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:12.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:56.9pt;\" width=\"76\" nowrap valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e666.58\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:12.5pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:12.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:96.55pt;\" width=\"129\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e37.5\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:12.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:57.8pt;\" width=\"77\" nowrap valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e119.34\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:12.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:57.8pt;\" width=\"77\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e16.5%\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:12.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:56.9pt;\" width=\"76\" nowrap valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e372.54\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:12.5pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:12.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:96.55pt;\" width=\"129\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e18.75\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:12.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:57.8pt;\" width=\"77\" nowrap valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e67.44\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:12.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:57.8pt;\" width=\"77\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e16.6%\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:12.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:56.9pt;\" width=\"76\" nowrap valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e167.04\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:12.5pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:12.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:96.55pt;\" width=\"129\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" 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1.0pt;border-top:none;height:12.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:56.9pt;\" width=\"76\" nowrap valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e95.17\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:12.5pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:12.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:96.55pt;\" width=\"129\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e4.69\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:12.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:57.8pt;\" width=\"77\" nowrap valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e41.885\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:12.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:57.8pt;\" width=\"77\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2.7%\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:12.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:56.9pt;\" width=\"76\" nowrap valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e65.85\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:12.5pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:12.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:96.55pt;\" width=\"129\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2.34\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:12.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:57.8pt;\" width=\"77\" nowrap valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e42.345\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:12.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:57.8pt;\" width=\"77\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e0.6%\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:12.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:56.9pt;\" width=\"76\" nowrap valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e67.67\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:12.5pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:12.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:96.55pt;\" width=\"129\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1.17\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:12.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:57.8pt;\" width=\"77\" nowrap valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e39.86\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:12.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:57.8pt;\" width=\"77\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e3.6%\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:12.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:56.9pt;\" width=\"76\" nowrap valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e57.83\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:12.5pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:12.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:96.55pt;\" width=\"129\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e0 (DMSO)\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:12.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:57.8pt;\" width=\"77\" nowrap valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e40.085\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:12.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:57.8pt;\" width=\"77\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e0.4%\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:12.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:56.9pt;\" width=\"76\" nowrap valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e58.72\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:12.5pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:12.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:96.55pt;\" width=\"129\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eEu only\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:12.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:57.8pt;\" width=\"77\" nowrap valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e25.255\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:12.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:57.8pt;\" width=\"77\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e7.0%\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:12.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:56.9pt;\" width=\"76\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e \u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"500T","offer_id":42332758278219,"sku":"UA086019-500T","price":2180.0,"currency_code":"USD","in_stock":true},{"title":"2500T","offer_id":42332758310987,"sku":"UA086019-2500T","price":8175.0,"currency_code":"USD","in_stock":true},{"title":"10000T","offer_id":42332758343755,"sku":"UA086019-10000T","price":21800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/c967a5369e5e4f139e4bfb740b84b8d9.png?v=1793423037"},{"product_id":"unione-tr-fret-human-bcl6crbn-protac-binding-kit-ua086021","title":"UniOne® TR-FRET Human BCL6\/CRBN PROTAC Binding Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan style=\"font-size:11.0pt;color:#0F1115\"\u003eStore the kit at -80°C or below. When storedappropriately, the reagents remain stable until the expiration date marked onthe label. Once thawed, BCL6 and CRBN stock solutions can undergo oneadditional freeze-thaw cycle; labeled antibody solutions can undergo oneadditional freeze-thaw cycle. To avoid repeated freeze-thaw cycles, it isrecommended to aliquot the remaining stock solutions into single-use containersfor storage at -80°C. Thawed diluents and Detection Buffer can be stored at2-8°C.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-size:11.0pt;color:#0F1115\"\u003eThis kit uses Homogeneous Time-Resolved Fluorescence(TR-FRET) technology to measure the molecular glue ARV-393 and testcompound-mediated interaction between the DDB1\/CRBN complex and BCL6. Thismethod enables simple and rapid high-throughput screening of small moleculescapable of mediating the interaction between the DDB1\/CRBN complex and BCL6.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:11.0pt;color:#0F1115\"\u003eAs shown, the interaction between DDB1\/CRBN and BCL6 isdetected using a Eu-labeled Anti-Tag1 Antibody (TR-FRET donor) and anAcceptor-labeled Anti-Tag2 Antibody (TR-FRET acceptor). As the molecular glueARV-393 mediates the interaction between DDB1\/CRBN and BCL6, the donor andacceptor antibodies are brought into proximity. Excitation of the donorantibody triggers Fluorescence Resonance Energy Transfer (FRET) to the acceptorantibody, resulting in a specific emission signal at 665 nm. This specificsignal is proportional to the degree of interaction mediated by ARV-393 betweenDDB1\/CRBN and BCL6. This homogeneous assay is simple to perform and requires nowashing steps.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/product.template\/20289289\/9244b132-962e-4998-8ab0-699b7ea1602d.png?access_token=1e46193c-b79e-4f03-aa69-29ed8eac8258\" class=\"img img-fluid o_we_custom_image\" style=\"width: 50%;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"background-color:white;margin:12.0pt 0cm;text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:#0F1115;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eTable 1. Kit Components and Storage Conditions\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:none;\" border=\"1\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003cthead\u003e\u003ctr\u003e\n\u003cth style=\"border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e100 TESTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e500 TESTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e2500 TESTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e10000 TESTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003eStorage Temperature\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eTag1-DDB1\/CRBN protein,\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e250μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1000μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eTag2- BCL6 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e250μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1000μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eARV-393 (100 μM)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e5μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e25μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e125μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e500μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eAnti-Tag1 Eu dye antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e250μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1000μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eAnti-Tag2 Ac antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e20×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e25μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e125μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e625μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2500μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 12.0pt 7.5pt 0cm;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2ml\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10ml\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 12.0pt 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e40mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 0cm 7.5pt 12.0pt;\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\u003c\/figure\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e1\u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003eReagent Preparation\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;text-autospace:none;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eBefore use, thaw all reagents at room temperature (equilibrate at room temperature for at least\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e30min\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e).\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e384\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e shallow well plate reaction system is\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e20uL\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e (reagent volumes for the reaction system are shown in the table), calculate the required volume before preparation and prepare as needed; the following preparation is for reference only, taking\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e500\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e times as an example.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;text-autospace:none;\" align=\"left\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:center;text-autospace:none;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eTable\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2. \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eReagent Preparation and Volumes\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:none;margin-left:10.15pt;\" border=\"1\" cellspacing=\"0\" cellpadding=\"0\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:114.2pt;\" width=\"152\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003eReagent Name\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:232.85pt;\" width=\"310\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003ePreparation\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:61.7pt;\" width=\"82\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003eVolume (\u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003eμL\u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003e)\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:114.2pt;\" width=\"152\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eARV-393\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:232.85pt;\" width=\"310\" valign=\"top\"\u003e\n\u003cp\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eAccording to the reaction system, dilute the compound with\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e to the desired concentration.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eEnsure consistent\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDMSO\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e content in all detection wells.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:61.7pt;\" width=\"82\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:114.2pt;\" width=\"152\"\u003e\u003cp style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eTag1-DDB1\/CRBN protein,\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:232.85pt;\" width=\"310\" valign=\"top\"\u003e\n\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eTake\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e80μL Tag1-DDB1\/CRBN protein\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e stock solution, dilute with\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e to\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2mL,\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003emix well and set aside.\u003c\/strong\u003e\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:61.7pt;\" width=\"82\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:114.2pt;\" width=\"152\"\u003e\u003cp style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eTag2- BCL6 protein\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:232.85pt;\" width=\"310\" valign=\"top\"\u003e\n\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eTake\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e20μL Tag2- BRD4 protein\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e stock solution, dilute with\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e to\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2mL,\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003emix well and set aside.\u003c\/strong\u003e\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:61.7pt;\" width=\"82\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:114.2pt;\" width=\"152\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eAntibody Mix\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:232.85pt;\" width=\"310\" valign=\"top\"\u003e\n\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eTake\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50μL Anti-Tag1 Eu dye antibody\u003c\/span\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e,\u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e dilute with\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e to\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2500μL\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e, mix well; take\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50μL Anti-Tag2 LA\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e, dilute with\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e to\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2500μL\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e, mix well,\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1:1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e mix to form\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eAntibody Mix\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e.\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:61.7pt;\" width=\"82\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp class=\"Default\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e1.1 \u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003eGradient Dilution of Test Samples\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;text-indent:21.0pt;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eUse\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e×\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e to prepare test samples and standards. If the sample stock solution is\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDMSO\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e, it is recommended to maintain consistent\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDMSO \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003econtent in the system,\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e(\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eThe Detection buffer does not contain\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDMSO\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e, please add\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDMSO\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e to\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e×\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e according to the dilution requirements of the standard or test sample) with a maximum of\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2%\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e. The\u003c\/span\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eARV-393\u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e standard in the kit is a\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e100μM DMSO \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003estock solution. Please prepare the total amount of standard based on experimental needs; the following content is for reference only.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;text-indent:21.0pt;\" align=\"left\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eTable\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e3. \u003c\/span\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eARV-393\u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e Gradient Dilution\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;\" border=\"0\" cellspacing=\"0\" cellpadding=\"0\" width=\"100%\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height:1.0pt;\"\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;height:1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:10.14%;\" width=\"10%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid windowtext 1.0pt;height:1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:18.82%;\" width=\"18%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eARV-393\u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e Final Concentration (\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003enM\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid windowtext 1.0pt;height:1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:19.76%;\" width=\"19%\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eARV-393\u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e Preparation Concentration (\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003enM\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid windowtext 1.0pt;height:1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:51.28%;\" width=\"51%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003ePreparation Method\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:1.0pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:10.14%;\" width=\"10%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e①\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:80.15pt;\" width=\"107\" nowrap\u003e\u003cp style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e400\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:84.2pt;\" width=\"112\"\u003e\u003cp style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e4000\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:51.28%;\" width=\"51%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e4μL 100μM stock solution +96μL Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:1.0pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:10.14%;\" width=\"10%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e②\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:80.15pt;\" width=\"107\" nowrap\u003e\u003cp style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e200\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:84.2pt;\" width=\"112\"\u003e\u003cp style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2000\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:51.28%;\" width=\"51%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50μL ① +50μL Detection buffer (1%DMSO)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:1.0pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:10.14%;\" width=\"10%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e③\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:80.15pt;\" width=\"107\" nowrap\u003e\u003cp style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e100\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:84.2pt;\" width=\"112\"\u003e\u003cp style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1000\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:51.28%;\" width=\"51%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50μL ② +50μL Detection buffer (1%DMSO)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:1.0pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:10.14%;\" width=\"10%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e④\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:80.15pt;\" width=\"107\" nowrap\u003e\u003cp style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:84.2pt;\" width=\"112\"\u003e\u003cp style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e500\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:51.28%;\" width=\"51%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50μL ③ +50μL Detection buffer (1%DMSO)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:1.0pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:10.14%;\" width=\"10%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e⑤\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:80.15pt;\" width=\"107\" nowrap\u003e\u003cp style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e25\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:84.2pt;\" width=\"112\"\u003e\u003cp style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e250\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:51.28%;\" width=\"51%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50μL ④ +50μL Detection buffer (1%DMSO)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:1.0pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:10.14%;\" width=\"10%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e⑥\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:80.15pt;\" width=\"107\" nowrap\u003e\u003cp style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e12.5\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:84.2pt;\" width=\"112\"\u003e\u003cp style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e125\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:51.28%;\" width=\"51%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50μL ⑤ +50μL Detection buffer (1%DMSO)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:1.0pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:10.14%;\" width=\"10%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e⑦\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:80.15pt;\" width=\"107\" nowrap\u003e\u003cp style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e6.25\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:84.2pt;\" width=\"112\"\u003e\u003cp style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e62.5\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:51.28%;\" width=\"51%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50μL ⑥ +50μL Detection buffer (1%DMSO)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:1.0pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:10.14%;\" width=\"10%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e⑧\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:80.15pt;\" width=\"107\" nowrap\u003e\u003cp style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e3.125\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:84.2pt;\" width=\"112\"\u003e\u003cp style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e31.25\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:51.28%;\" width=\"51%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50μL ⑦ +50μL Detection buffer (1%DMSO)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:1.0pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:10.14%;\" width=\"10%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e⑨\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:80.15pt;\" width=\"107\" nowrap\u003e\u003cp style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1.5625\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:84.2pt;\" width=\"112\"\u003e\u003cp style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e15.625\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:51.28%;\" width=\"51%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50μL ⑧ +50μL Detection buffer (1%DMSO)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:1.0pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:10.14%;\" width=\"10%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e⑩\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:80.15pt;\" width=\"107\" nowrap\u003e\u003cp style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e0.78125\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:84.2pt;\" width=\"112\"\u003e\u003cp style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e7.8125\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:51.28%;\" width=\"51%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50μL ⑨ +50μL Detection buffer (1%DMSO)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:1.0pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:10.14%;\" width=\"10%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eBlank\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:18.82%;\" width=\"18%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:19.76%;\" width=\"19%\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:51.28%;\" width=\"51%\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e100μL Detection buffer (1%DMSO)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e2 \u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003eSample Addition and Controls\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;text-autospace:none;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2.1 \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eSample addition order for experimental wells:\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e4uL Tag1-DDB1\/CRBN protein\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e,\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e4uL L Tag2- BCL6 protein\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e,\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2uL ARV-393 \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eor compounds to be tested,\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10uL\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e mixed\u003c\/span\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eAntibody Mix\u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e, add sequentially to\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e384\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e shallow well plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;text-autospace:none;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2.2 \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eNegative control wells (\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eBlank\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e):\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e ARV-393 \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eor compounds to be tested replaced with\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2uL\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e of\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDMSO\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e solution with the same percentage content.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2.3 NC\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e:\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e15uL Detection buffer\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e plus\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e5uL\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e diluted\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eAnti-Tag1 Eu dye antibody\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eTable\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e4. \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eSample Addition and Controls\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:none;\" border=\"1\" cellspacing=\"0\" cellpadding=\"0\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:59.6pt;\" width=\"79\" valign=\"top\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:98.5pt;\" width=\"131\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eNegative Control\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:97.45pt;\" width=\"130\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003ePositive Control\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:93.45pt;\" width=\"125\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eSample\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:76.95pt;\" width=\"103\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eNC\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:59.6pt;\" rowspan=\"4\" width=\"79\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eStep 1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:98.5pt;\" width=\"131\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e diluted\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDMSO\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e (\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1%DMSO\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:97.45pt;\" width=\"130\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e4μM ARV-393\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:93.45pt;\" width=\"125\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003egradient diluted test samples\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:76.95pt;\" rowspan=\"4\" width=\"103\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e15μL 1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e× Detection buffer (\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1%DMSO\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eplus\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e5uL 1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e×\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eAnti-Tag1 Eu dye antibody\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:289.4pt;\" colspan=\"3\" width=\"386\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eTag1-DDB1\/CRBN protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:289.4pt;\" colspan=\"3\" width=\"386\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e4uL L Tag2- BCL6 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:289.4pt;\" colspan=\"3\" width=\"386\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10μL Antibody Mix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:59.6pt;\" width=\"79\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eStep 2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:366.35pt;\" colspan=\"4\" width=\"488\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eSeal plate wells with sealing film\u003cstrong\u003e,\u003c\/strong\u003e incubate at room temperature for\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2h\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e3.\u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003eDetection\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;text-autospace:none;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eDetect on a microplate reader compatible with\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eTR-FRET\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e (excitation wavelength is\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e320nm\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e, detect emission wavelengths of\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e620nm\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e and\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e665nm\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;text-autospace:none;\" align=\"left\"\u003e \u003c\/p\u003e\n\u003cp style=\"background-color:white;line-height:150%;margin:0cm 9.0pt 0cm 0cm;\"\u003e\u003cspan style=\"color:#7030A0;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;line-height:150%;\"\u003e\u003cstrong\u003e[Result Calculation]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"Default\"\u003e\u003cspan style=\"color:windowtext;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1. \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eCalculate signal value:\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e665nm\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e fluorescence signal ratio\u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e620nm\u003c\/span\u003e\u003cspan style=\"color:windowtext;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e fluorescence signal multiplied by\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10000\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e, i.e.,\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"Default\"\u003e\u003cspan style=\"color:windowtext;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eSignal Value\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e=\u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e \u003c\/span\u003e\u003cspan style=\"color:windowtext;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e(665\/620)*10000\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"Default\"\u003e\u003cspan style=\"color:windowtext;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2. \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eCalculate δ value based on signal value:\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"Default\"\u003e\u003cspan style=\"color:windowtext;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eδ Value\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e= (Std-NC)\/NC*100\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;text-autospace:none;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e3.\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eCalculate\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eCV\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e (\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e%\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eCV\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e (\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e%\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e)\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e= Standard Deviation\/Mean Ratio×100%\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"background-color:white;line-height:150%;margin:0cm 9.0pt 0cm 0cm;\"\u003e\u003cspan style=\"color:#7030A0;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;line-height:150%;\"\u003e\u003cstrong\u003e[Data Example]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eThe following data cannot replace data obtained from experiments and is only for illustration; results may vary depending on\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eTR-FRET\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e compatible instruments.\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:none;width:272.25pt;\" border=\"1\" cellspacing=\"0\" cellpadding=\"0\" width=\"363\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height:13.85pt;\"\u003e\n\u003ctd style=\"background-color:white;border:solid windowtext 1.0pt;height:13.85pt;padding:0cm 5.4pt 0cm 5.4pt;width:72.3pt;\" width=\"96\"\u003e\u003cp style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eARV-393 ,nM\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:white;border-left:none;border:solid windowtext 1.0pt;height:13.85pt;padding:0cm 5.4pt 0cm 5.4pt;width:67.15pt;\" width=\"90\"\u003e\u003cp style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eRatio\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid windowtext 1.0pt;height:13.85pt;padding:0cm 5.4pt 0cm 5.4pt;width:50.85pt;\" width=\"68\" nowrap\u003e\u003cp style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eCV(%)\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:white;border-left:none;border:solid windowtext 1.0pt;height:13.85pt;padding:0cm 5.4pt 0cm 5.4pt;width:82.1pt;\" width=\"109\"\u003e\u003cp style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eNet Signal\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:15.35pt;\"\u003e\n\u003ctd style=\"background-color:white;border-top:none;border:solid windowtext 1.0pt;height:15.35pt;padding:0cm 5.4pt 0cm 5.4pt;width:72.3pt;\" width=\"96\"\u003e\u003cp style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e400\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:white;border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:15.35pt;padding:0cm 5.4pt 0cm 5.4pt;width:67.15pt;\" width=\"90\"\u003e\u003cp style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1568.00\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:15.35pt;padding:0cm 5.4pt 0cm 5.4pt;\" nowrap\u003e\u003cp style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e30%\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:white;border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:15.35pt;padding:0cm 5.4pt 0cm 5.4pt;width:82.1pt;\" width=\"109\"\u003e\u003cp style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e317.47\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:15.35pt;\"\u003e\n\u003ctd style=\"background-color:white;border-top:none;border:solid windowtext 1.0pt;height:15.35pt;padding:0cm 5.4pt 0cm 5.4pt;width:72.3pt;\" width=\"96\"\u003e\u003cp style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e200\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:white;border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:15.35pt;padding:0cm 5.4pt 0cm 5.4pt;width:67.15pt;\" width=\"90\"\u003e\u003cp style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e3186.00\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:15.35pt;padding:0cm 5.4pt 0cm 5.4pt;\" nowrap\u003e\u003cp style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e9%\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:white;border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:15.35pt;padding:0cm 5.4pt 0cm 5.4pt;width:82.1pt;\" width=\"109\"\u003e\u003cp style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e748.24\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:15.35pt;\"\u003e\n\u003ctd style=\"background-color:white;border-top:none;border:solid windowtext 1.0pt;height:15.35pt;padding:0cm 5.4pt 0cm 5.4pt;width:72.3pt;\" width=\"96\"\u003e\u003cp style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e100\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:white;border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:15.35pt;padding:0cm 5.4pt 0cm 5.4pt;width:67.15pt;\" width=\"90\"\u003e\u003cp style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e3240.50\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:15.35pt;padding:0cm 5.4pt 0cm 5.4pt;\" nowrap\u003e\u003cp style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2%\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:white;border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:15.35pt;padding:0cm 5.4pt 0cm 5.4pt;width:82.1pt;\" width=\"109\"\u003e\u003cp style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e762.75\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:15.35pt;\"\u003e\n\u003ctd style=\"background-color:white;border-top:none;border:solid windowtext 1.0pt;height:15.35pt;padding:0cm 5.4pt 0cm 5.4pt;width:72.3pt;\" width=\"96\"\u003e\u003cp style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:white;border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:15.35pt;padding:0cm 5.4pt 0cm 5.4pt;width:67.15pt;\" width=\"90\"\u003e\u003cp style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1637.50\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:15.35pt;padding:0cm 5.4pt 0cm 5.4pt;\" nowrap\u003e\u003cp style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e31%\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:white;border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:15.35pt;padding:0cm 5.4pt 0cm 5.4pt;width:82.1pt;\" width=\"109\"\u003e\u003cp style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e335.97\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:15.35pt;\"\u003e\n\u003ctd style=\"background-color:white;border-top:none;border:solid windowtext 1.0pt;height:15.35pt;padding:0cm 5.4pt 0cm 5.4pt;width:72.3pt;\" width=\"96\"\u003e\u003cp style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e25\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:white;border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:15.35pt;padding:0cm 5.4pt 0cm 5.4pt;width:67.15pt;\" width=\"90\"\u003e\u003cp style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e575.90\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:15.35pt;padding:0cm 5.4pt 0cm 5.4pt;\" nowrap\u003e\u003cp style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" 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style=\"text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e71\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"500T","offer_id":42332758474827,"sku":"UA086021-500T","price":2180.0,"currency_code":"USD","in_stock":true},{"title":"2500T","offer_id":42332758507595,"sku":"UA086021-2500T","price":8175.0,"currency_code":"USD","in_stock":true},{"title":"10000T","offer_id":42332758540363,"sku":"UA086021-10000T","price":21800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/2d0a143c3f9b40b18477d786d99961c4.png?v=1793423044"},{"product_id":"unione-tr-fret-human-vav1crbn-protac-binding-kit-ua086022","title":"UniOne® TR-FRET Human VAV1\/CRBN PROTAC Binding Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-80°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.0pt;\"\u003eThe kit employs homogeneous time-resolved fluorescence (TR-FRET) technology to measure the interaction between the DDB1\/CRBN complex and VAV1 mediated by the molecular glue MRT-6160 or test compounds. This method provides a high-throughput format for the simple and rapid detection of small molecules capable of mediating the interaction between the DDB1\/CRBN complex and VAV1.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.0pt;\"\u003eAs shown, the interaction between DDB1\/CRBN and VAV1 is detected using a Eu-labeled anti-Tag1 antibody (TR-FRET donor) and an Accepter-labeled anti-Tag2 antibody (TR-FRET acceptor). Due to the molecular glue MRT-6160 mediating the interaction between DDB1\/CRBN and VAV1, the donor and acceptor antibodies are brought into close proximity. Excitation of the donor antibody triggers fluorescence resonance energy transfer (FRET) to the acceptor antibody, causing the acceptor antibody to specifically emit a signal at a wavelength of 665 nm. This specific signal is proportional to the extent of the interaction mediated by MRT-6160 between DDB1\/CRBN and VAV1. This homogeneous assay is simple to perform and requires no washing steps.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"img img-fluid o_we_custom_image\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/product.template\/20289303\/93cfcbbd-4d52-449c-bdbf-e96f48c6043b.png?access_token=11f4906d-31b8-409f-b7d3-21d46539effe\" alt=\"\"\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;margin-left:49.3pt;width:509.8pt;\" border=\"0\" cellspacing=\"0\" cellpadding=\"0\" width=\"680\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height:28.75pt;\"\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:solid windowtext 1.0pt;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:137.9pt;\" width=\"184\"\u003e\u003cp style=\"layout-grid-mode:both;text-align:center;text-autospace:ideograph-numeric ideograph-other;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;layout-grid-mode:both;'\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border-left:none;border:solid windowtext 1.0pt;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:66.0pt;\" width=\"88\"\u003e\u003cp style=\"layout-grid-mode:both;text-align:center;text-autospace:ideograph-numeric ideograph-other;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;layout-grid-mode:both;'\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border-left:none;border:solid windowtext 1.0pt;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:58.65pt;\" width=\"78\"\u003e\u003cp style=\"layout-grid-mode:both;text-align:center;text-autospace:ideograph-numeric ideograph-other;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;layout-grid-mode:both;' lang=\"EN-US\"\u003e\u003cstrong\u003e100T\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border-left:none;border:solid windowtext 1.0pt;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:58.65pt;\" width=\"78\"\u003e\u003cp style=\"layout-grid-mode:both;text-align:center;text-autospace:ideograph-numeric ideograph-other;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;layout-grid-mode:both;' lang=\"EN-US\"\u003e\u003cstrong\u003e500T\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border-left:none;border:solid windowtext 1.0pt;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:58.65pt;\" width=\"78\"\u003e\u003cp style=\"layout-grid-mode:both;text-align:center;text-autospace:ideograph-numeric ideograph-other;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;layout-grid-mode:both;' lang=\"EN-US\"\u003e\u003cstrong\u003e2500T\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border-left:none;border:solid windowtext 1.0pt;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:58.65pt;\" width=\"78\"\u003e\u003cp style=\"layout-grid-mode:both;text-align:center;text-autospace:ideograph-numeric ideograph-other;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;layout-grid-mode:both;' lang=\"EN-US\"\u003e\u003cstrong\u003e10000T\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border-left:none;border:solid windowtext 1.0pt;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:71.3pt;\" width=\"95\"\u003e\u003cp style=\"layout-grid-mode:both;text-align:center;text-autospace:ideograph-numeric ideograph-other;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;layout-grid-mode:both;'\u003e\u003cstrong\u003eStorage Temperature\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:28.75pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:137.9pt;\" width=\"184\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003eTag1-DDB1\/CRBN protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:66.0pt;\" width=\"88\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:58.65pt;\" width=\"78\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e5μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:58.65pt;\" width=\"78\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e20μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:58.65pt;\" width=\"78\"\u003e\u003cp style=\"line-height:115%;text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;line-height:115%;' lang=\"EN-US\"\u003e100μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:58.65pt;\" width=\"78\"\u003e\u003cp style=\"line-height:115%;text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;line-height:115%;' lang=\"EN-US\"\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:71.3pt;\" width=\"95\"\u003e\u003cp style=\"line-height:115%;text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;line-height:115%;' lang=\"EN-US\"\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:28.75pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:137.9pt;\" width=\"184\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003eTag2- VAV1 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:66.0pt;\" width=\"88\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:58.65pt;\" width=\"78\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e5μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:58.65pt;\" width=\"78\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e20μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:58.65pt;\" width=\"78\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e100μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:58.65pt;\" width=\"78\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:71.3pt;\" width=\"95\"\u003e\u003cp style=\"line-height:115%;text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;line-height:115%;' lang=\"EN-US\"\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:28.75pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:137.9pt;\" width=\"184\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003eMRT-6160 （200μM）\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:66.0pt;\" width=\"88\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:58.65pt;\" width=\"78\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e5μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:58.65pt;\" width=\"78\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:58.65pt;\" width=\"78\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:58.65pt;\" width=\"78\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e200μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:71.3pt;\" width=\"95\"\u003e\u003cp style=\"line-height:115%;text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;line-height:115%;' lang=\"EN-US\"\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:28.75pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:137.9pt;\" width=\"184\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003eAnti-Tag1 Eu antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:66.0pt;\" width=\"88\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e50×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:58.65pt;\" width=\"78\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:58.65pt;\" width=\"78\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:58.65pt;\" width=\"78\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e250μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:58.65pt;\" width=\"78\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e1000μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:71.3pt;\" width=\"95\"\u003e\u003cp style=\"line-height:115%;text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;line-height:115%;' lang=\"EN-US\"\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:28.75pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:137.9pt;\" width=\"184\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003eAnti-Tag2 Ac antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:66.0pt;\" width=\"88\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:58.65pt;\" width=\"78\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e5μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:58.65pt;\" width=\"78\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e25μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:58.65pt;\" width=\"78\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e125μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:58.65pt;\" width=\"78\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e500μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:28.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:71.3pt;\" width=\"95\"\u003e\u003cp style=\"line-height:115%;text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;line-height:115%;' lang=\"EN-US\"\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:29.25pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:29.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:137.9pt;\" width=\"184\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:29.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:66.0pt;\" width=\"88\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e10×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:29.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:58.65pt;\" width=\"78\"\u003e\u003cp style=\"line-height:115%;text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;line-height:115%;' lang=\"EN-US\"\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:29.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:58.65pt;\" width=\"78\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e2mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:29.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:58.65pt;\" width=\"78\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e10mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:29.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:58.65pt;\" width=\"78\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e40mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:29.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:71.3pt;\" width=\"95\"\u003e\u003cp style=\"line-height:115%;text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;line-height:115%;' lang=\"EN-US\"\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp style=\"line-height:115%;margin-top:3.0pt;text-indent:39.6pt;\"\u003e\u003cspan style=\"font-size:10.0pt;letter-spacing:-.05pt;line-height:115%;\"\u003eNote: Aliquot immediately after the first thaw and store at the recommended storage temperature. Avoid storage after dilution and repeated freeze-thaw cycles.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\u003cdiv class=\"WordSection1\"\u003e\n\u003cp style=\"line-height:115%;margin:9.65pt 0cm .0001pt 52.05pt;\"\u003e\u003cspan style=\"letter-spacing:-.3pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e1\u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"letter-spacing:-.3pt;\"\u003e\u003cstrong\u003e. Reagent Preparation\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"line-height:115%;margin:8.4pt 39.1pt .0001pt 51.05pt;text-indent:20.0pt;\"\u003e\u003cspan style=\"font-size:10.0pt;line-height:115%;\" lang=\"EN-US\"\u003e1.1 \u003c\/span\u003e\u003cspan style=\"font-size:10.0pt;line-height:115%;\"\u003eThaw all reagents at room temperature before use (equilibrate at room temperature for at least\u003c\/span\u003e\u003cspan style=\"font-size:10.0pt;line-height:115%;\" lang=\"EN-US\"\u003e30min\u003c\/span\u003e\u003cspan style=\"font-size:10.0pt;line-height:115%;\"\u003e). The reaction system for the 384-well shallow plate is\u003c\/span\u003e\u003cspan style=\"font-size:10.0pt;line-height:115%;\" lang=\"EN-US\"\u003e20μL\u003c\/span\u003e\u003cspan style=\"font-size:10.0pt;line-height:115%;\"\u003e (reagent volumes for the reaction system are shown in the table). Calculate the required volume for the experiment before preparation and prepare as needed; the following preparation is for reference only, using\u003c\/span\u003e\u003cspan style=\"font-size:10.0pt;line-height:115%;\" lang=\"EN-US\"\u003e500\u003c\/span\u003e\u003cspan style=\"font-size:10.0pt;line-height:115%;\"\u003eassays as an example.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"line-height:115%;margin:8.4pt 39.1pt .0001pt 51.05pt;text-align:center;text-indent:19.8pt;\"\u003e\u003cspan style=\"font-size:10.0pt;letter-spacing:-.05pt;line-height:115%;\"\u003eTable\u003c\/span\u003e\u003cspan style=\"font-size:10.0pt;letter-spacing:-.05pt;line-height:115%;\" lang=\"EN-US\"\u003e1. \u003c\/span\u003e\u003cspan style=\"font-size:10.0pt;letter-spacing:-.05pt;line-height:115%;\"\u003eReagent Preparation\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:none;margin-left:55.05pt;width:505.0pt;\" border=\"1\" cellspacing=\"0\" cellpadding=\"0\" width=\"673\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height:30.0pt;\"\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:solid windowtext 1.0pt;height:30.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:119.0pt;\" width=\"159\"\u003e\u003cp style=\"layout-grid-mode:both;text-align:center;text-autospace:ideograph-numeric ideograph-other;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;layout-grid-mode:both;'\u003e\u003cstrong\u003eReagent Name\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border-left:none;border:solid windowtext 1.0pt;height:30.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:300.35pt;\" width=\"400\"\u003e\u003cp style=\"layout-grid-mode:both;text-align:center;text-autospace:ideograph-numeric ideograph-other;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;layout-grid-mode:both;'\u003e\u003cstrong\u003ePreparation\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border-left:none;border:solid windowtext 1.0pt;height:30.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:85.65pt;\" width=\"114\"\u003e\u003cp style=\"layout-grid-mode:both;text-align:center;text-autospace:ideograph-numeric ideograph-other;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;layout-grid-mode:both;'\u003e\u003cstrong\u003eVolume per Assay Well μ\u003c\/strong\u003e\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;layout-grid-mode:both;' lang=\"EN-US\"\u003e\u003cstrong\u003eL\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:30.0pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:30.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:119.0pt;\" width=\"159\"\u003e\u003cp style=\"layout-grid-mode:both;line-height:115%;text-align:center;text-autospace:ideograph-numeric ideograph-other;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;layout-grid-mode:both;line-height:115%;' lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:30.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:300.35pt;\" width=\"400\"\u003e\u003cp style=\"layout-grid-mode:both;line-height:115%;text-align:justify;text-autospace:ideograph-numeric ideograph-other;text-justify:inter-ideograph;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;layout-grid-mode:both;line-height:115%;'\u003eTake\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;layout-grid-mode:both;line-height:115%;' lang=\"EN-US\"\u003e2mL 10\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;layout-grid-mode:both;line-height:115%;'\u003e×\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;layout-grid-mode:both;line-height:115%;' lang=\"EN-US\"\u003e Detection buffer1\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;layout-grid-mode:both;line-height:115%;'\u003eadd\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;layout-grid-mode:both;line-height:115%;' lang=\"EN-US\"\u003e18mL\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;layout-grid-mode:both;line-height:115%;'\u003edeionized water, dilute to\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;layout-grid-mode:both;line-height:115%;' lang=\"EN-US\"\u003e1×\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;layout-grid-mode:both;line-height:115%;'\u003e, mix well and set aside.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:30.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:85.65pt;\" width=\"114\"\u003e\u003cp style=\"layout-grid-mode:both;line-height:115%;text-align:center;text-autospace:ideograph-numeric ideograph-other;\"\u003e\u003cspan style='font-family:\"Times New Roman\",serif;font-size:9.0pt;layout-grid-mode:both;line-height:115%;' lang=\"EN-US\"\u003e-\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:30.0pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:30.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:119.0pt;\" width=\"159\"\u003e\u003cp style=\"line-height:115%;text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;line-height:115%;' lang=\"EN-US\"\u003eMRT-6160\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:30.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:300.35pt;\" width=\"400\" valign=\"top\"\u003e\n\u003cp style=\"line-height:115%;text-align:justify;text-justify:inter-ideograph;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;line-height:115%;' lang=\"EN-US\"\u003eAccording to the reaction system, dilute the compound to the desired concentration using 1× Detection buffer.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"line-height:115%;text-align:justify;text-justify:inter-ideograph;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;line-height:115%;' lang=\"EN-US\"\u003eEnsure consistent DMSO concentration across all assay wells.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:30.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:85.65pt;\" width=\"114\"\u003e\u003cp style=\"line-height:115%;text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;line-height:115%;' lang=\"EN-US\"\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:30.0pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:30.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:119.0pt;\" width=\"159\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003eTag1-DDB1\/CRBN protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:30.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:300.35pt;\" width=\"400\" valign=\"top\"\u003e\u003cp style=\"line-height:115%;text-align:justify;text-justify:inter-ideograph;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;line-height:115%;' lang=\"EN-US\"\u003eTake 20μL Tag1-DDB1\/CRBN protein stock solution, dilute to 2mL with 1× Detection buffer,\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;font-weight:normal;line-height:115%;' lang=\"EN-US\"\u003e\u003cstrong\u003emix well and set aside.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:30.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:85.65pt;\" width=\"114\"\u003e\u003cp style=\"line-height:115%;text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;line-height:115%;' lang=\"EN-US\"\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:30.0pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:30.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:119.0pt;\" width=\"159\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003eTag2- VAV1 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:30.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:300.35pt;\" width=\"400\" valign=\"top\"\u003e\u003cp style=\"line-height:115%;text-align:justify;text-justify:inter-ideograph;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;line-height:115%;' lang=\"EN-US\"\u003eTake 20μL Tag2- VAV1 protein stock solution, dilute to 2mL with 1× Detection buffer,\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;font-weight:normal;line-height:115%;' lang=\"EN-US\"\u003e\u003cstrong\u003emix well and set aside.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:30.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:85.65pt;\" width=\"114\"\u003e\u003cp style=\"line-height:115%;text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;line-height:115%;' lang=\"EN-US\"\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:30.0pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:30.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:119.0pt;\" width=\"159\"\u003e\u003cp style=\"line-height:115%;text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;line-height:115%;' lang=\"EN-US\"\u003eAntibody Mix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:30.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:300.35pt;\" width=\"400\" valign=\"top\"\u003e\u003cp style=\"line-height:115%;text-align:justify;text-justify:inter-ideograph;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;line-height:115%;' lang=\"EN-US\"\u003eTake 50μL Anti-Tag1 Eu antibody stock solution, add 2.45mL of 1× Detection buffer,\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;font-weight:normal;line-height:115%;' lang=\"EN-US\"\u003e\u003cstrong\u003e mix well;\u003c\/strong\u003e\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;line-height:115%;' lang=\"EN-US\"\u003etake 25μL Anti-Tag2 Ac antibody stock solution, add 2.485mL of 1× Detection buffer,\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;font-weight:normal;line-height:115%;' lang=\"EN-US\"\u003e\u003cstrong\u003e mix well\u003c\/strong\u003e\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;font-weight:normal;line-height:115%;'\u003e\u003cstrong\u003e;\u003c\/strong\u003e\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;line-height:115%;' lang=\"EN-US\"\u003emix the two solutions in a 1:1 ratio to prepare the Antibody Mix.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:30.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:85.65pt;\" width=\"114\"\u003e\u003cp style=\"line-height:115%;text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;line-height:115%;' lang=\"EN-US\"\u003e10\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp style=\"line-height:115%;margin:8.4pt 39.1pt .0001pt 51.05pt;text-indent:20.0pt;\"\u003e\u003cspan style=\"font-size:10.0pt;line-height:115%;\" lang=\"EN-US\"\u003e1.2 \u003c\/span\u003e\u003cspan style=\"font-size:10.0pt;letter-spacing:-.05pt;line-height:115%;\"\u003ePositive Control Drug\u003c\/span\u003e\u003cspan style=\"font-size:10.0pt;letter-spacing:-.05pt;line-height:115%;\" lang=\"EN-US\"\u003e\/\u003c\/span\u003e\u003cspan style=\"font-size:10.0pt;letter-spacing:-.05pt;line-height:115%;\"\u003eTest Sample Gradient\u003c\/span\u003e\u003cspan style=\"font-size:10.0pt;line-height:115%;\"\u003eDilution\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"line-height:115%;margin:8.4pt 39.1pt .0001pt 51.05pt;text-indent:20.0pt;\"\u003e\u003cspan style=\"font-size:10.0pt;line-height:115%;\"\u003eUsing\u003c\/span\u003e\u003cspan style=\"font-size:10.0pt;line-height:115%;\" lang=\"EN-US\"\u003eMRT-6160\u003c\/span\u003e\u003cspan style=\"font-size:10.0pt;line-height:115%;\"\u003e as an example, the diluent is\u003c\/span\u003e\u003cspan style=\"font-size:10.0pt;line-height:115%;\" lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-size:10.0pt;line-height:115%;\"\u003e×\u003c\/span\u003e\u003cspan style=\"font-size:10.0pt;line-height:115%;\" lang=\"EN-US\"\u003e Detection buffer\u003c\/span\u003e\u003cspan style=\"font-size:10.0pt;line-height:115%;\"\u003e, to minimize the impact of matrix effects, it is recommended to dilute using a solution with the same matrix as the test sample; adjust the test sample concentration according to actual conditions.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"line-height:115%;margin:8.4pt 39.1pt .0001pt 51.05pt;text-align:center;text-indent:19.8pt;\"\u003e\u003cspan style=\"font-size:10.0pt;letter-spacing:-.05pt;line-height:115%;\"\u003eTable\u003c\/span\u003e\u003cspan style=\"font-size:10.0pt;letter-spacing:-.05pt;line-height:115%;\" lang=\"EN-US\"\u003e2. \u003c\/span\u003e\u003cspan style=\"font-size:10.0pt;letter-spacing:-.05pt;line-height:115%;\"\u003ePositive Control Drug Gradient Dilution\u003c\/span\u003e\u003cspan style=\"font-size:10.0pt;line-height:115%;\"\u003e (adjust according to actual conditions)\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:none;margin-left:60.4pt;\" border=\"1\" cellspacing=\"0\" cellpadding=\"0\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height:25.15pt;\"\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:solid windowtext 1.0pt;height:25.15pt;padding:0cm 5.4pt 0cm 5.4pt;width:101.2pt;\" width=\"135\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border-left:none;border:solid windowtext 1.0pt;height:25.15pt;padding:0cm 5.4pt 0cm 5.4pt;width:85.95pt;\" width=\"115\" nowrap\u003e\u003cp style=\"layout-grid-mode:both;text-align:center;text-autospace:ideograph-numeric ideograph-other;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;layout-grid-mode:both;' lang=\"EN-US\"\u003e\u003cstrong\u003eMRT-6160\u003c\/strong\u003e\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;layout-grid-mode:both;'\u003e\u003cstrong\u003eFinal Concentration (\u003c\/strong\u003e\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;layout-grid-mode:both;' lang=\"EN-US\"\u003e\u003cstrong\u003enM\u003c\/strong\u003e\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;layout-grid-mode:both;'\u003e\u003cstrong\u003e)\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border-left:none;border:solid windowtext 1.0pt;height:25.15pt;padding:0cm 5.4pt 0cm 5.4pt;width:95.3pt;\" width=\"127\"\u003e\u003cp style=\"layout-grid-mode:both;text-align:center;text-autospace:ideograph-numeric ideograph-other;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;layout-grid-mode:both;' lang=\"EN-US\"\u003e\u003cstrong\u003eMRT-6160\u003c\/strong\u003e\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;layout-grid-mode:both;'\u003e\u003cstrong\u003ePreparation Concentration (\u003c\/strong\u003e\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;layout-grid-mode:both;' lang=\"EN-US\"\u003e\u003cstrong\u003enM\u003c\/strong\u003e\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;layout-grid-mode:both;'\u003e\u003cstrong\u003e)\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border-left:none;border:solid windowtext 1.0pt;height:25.15pt;padding:0cm 5.4pt 0cm 5.4pt;width:221.4pt;\" width=\"295\" nowrap\u003e\u003cp style=\"layout-grid-mode:both;text-align:center;text-autospace:ideograph-numeric ideograph-other;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;layout-grid-mode:both;'\u003e\u003cstrong\u003ePreparation Method\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:17.95pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:17.95pt;padding:0cm 5.4pt 0cm 5.4pt;width:101.2pt;\" width=\"135\" nowrap\u003e\u003col style=\"padding-left:28px;\"\u003e\u003cli\u003e \u003c\/li\u003e\u003c\/ol\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:17.95pt;padding:0cm 5.4pt 0cm 5.4pt;width:85.95pt;\" width=\"115\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e200\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:17.95pt;padding:0cm 5.4pt 0cm 5.4pt;width:95.3pt;\" width=\"127\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e2000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:17.95pt;padding:0cm 5.4pt 0cm 5.4pt;width:221.4pt;\" width=\"295\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e1μL 200μM stock solution+99μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:16.55pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:16.55pt;padding:0cm 5.4pt 0cm 5.4pt;width:101.2pt;\" width=\"135\" nowrap\u003e\u003col style=\"padding-left:28px;\" start=\"2\"\u003e\u003cli\u003e \u003c\/li\u003e\u003c\/ol\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:16.55pt;padding:0cm 5.4pt 0cm 5.4pt;width:85.95pt;\" width=\"115\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e100\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:16.55pt;padding:0cm 5.4pt 0cm 5.4pt;width:95.3pt;\" width=\"127\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e1000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:16.55pt;padding:0cm 5.4pt 0cm 5.4pt;width:221.4pt;\" width=\"295\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e50μL ① +50μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:17.6pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:17.6pt;padding:0cm 5.4pt 0cm 5.4pt;width:101.2pt;\" width=\"135\" nowrap\u003e\u003col style=\"padding-left:28px;\" start=\"3\"\u003e\u003cli\u003e \u003c\/li\u003e\u003c\/ol\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:17.6pt;padding:0cm 5.4pt 0cm 5.4pt;width:85.95pt;\" width=\"115\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e50\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:17.6pt;padding:0cm 5.4pt 0cm 5.4pt;width:95.3pt;\" width=\"127\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e500\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:17.6pt;padding:0cm 5.4pt 0cm 5.4pt;width:221.4pt;\" width=\"295\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e50μL ② +50μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:16.25pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:16.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:101.2pt;\" width=\"135\" nowrap\u003e\u003col style=\"padding-left:28px;\" start=\"4\"\u003e\u003cli\u003e \u003c\/li\u003e\u003c\/ol\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:16.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:85.95pt;\" width=\"115\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e25\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:16.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:95.3pt;\" width=\"127\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e250\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:16.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:221.4pt;\" width=\"295\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e50μL ③ +50μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:14.85pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:14.85pt;padding:0cm 5.4pt 0cm 5.4pt;width:101.2pt;\" width=\"135\" nowrap\u003e\u003col style=\"padding-left:28px;\" start=\"5\"\u003e\u003cli\u003e \u003c\/li\u003e\u003c\/ol\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:14.85pt;padding:0cm 5.4pt 0cm 5.4pt;width:85.95pt;\" width=\"115\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e12.5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:14.85pt;padding:0cm 5.4pt 0cm 5.4pt;width:95.3pt;\" width=\"127\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e125\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:14.85pt;padding:0cm 5.4pt 0cm 5.4pt;width:221.4pt;\" width=\"295\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e50μL ④ +50μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:4.5pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:4.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:101.2pt;\" width=\"135\" nowrap\u003e\u003col style=\"padding-left:28px;\" start=\"6\"\u003e\u003cli\u003e \u003c\/li\u003e\u003c\/ol\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:4.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:85.95pt;\" width=\"115\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e6.25\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:4.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:95.3pt;\" width=\"127\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e62.5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:4.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:221.4pt;\" width=\"295\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e50μL ⑤ +50μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:18.0pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:18.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:101.2pt;\" width=\"135\" nowrap\u003e\u003col style=\"padding-left:28px;\" start=\"7\"\u003e\u003cli\u003e \u003c\/li\u003e\u003c\/ol\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:18.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:85.95pt;\" width=\"115\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e3.125\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:18.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:95.3pt;\" width=\"127\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e31.25\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:18.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:221.4pt;\" width=\"295\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e50μL ⑥ +50μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:16.9pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:16.9pt;padding:0cm 5.4pt 0cm 5.4pt;width:101.2pt;\" width=\"135\" nowrap\u003e\u003col style=\"padding-left:28px;\" start=\"8\"\u003e\u003cli\u003e \u003c\/li\u003e\u003c\/ol\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:16.9pt;padding:0cm 5.4pt 0cm 5.4pt;width:85.95pt;\" width=\"115\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e1.56\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:16.9pt;padding:0cm 5.4pt 0cm 5.4pt;width:95.3pt;\" width=\"127\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e15.63\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:16.9pt;padding:0cm 5.4pt 0cm 5.4pt;width:221.4pt;\" width=\"295\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e50μL ⑦+50μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:16.9pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:16.9pt;padding:0cm 5.4pt 0cm 5.4pt;width:101.2pt;\" width=\"135\" nowrap\u003e\u003col style=\"padding-left:28px;\" start=\"9\"\u003e\u003cli\u003e \u003c\/li\u003e\u003c\/ol\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:16.9pt;padding:0cm 5.4pt 0cm 5.4pt;width:85.95pt;\" width=\"115\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e0.78\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:16.9pt;padding:0cm 5.4pt 0cm 5.4pt;width:95.3pt;\" width=\"127\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e7.81\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:16.9pt;padding:0cm 5.4pt 0cm 5.4pt;width:221.4pt;\" width=\"295\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e50μL\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;'\u003e⑧\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e+50μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:16.9pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:16.9pt;padding:0cm 5.4pt 0cm 5.4pt;width:101.2pt;\" width=\"135\" nowrap\u003e\u003col style=\"padding-left:28px;\" start=\"10\"\u003e\u003cli\u003e \u003c\/li\u003e\u003c\/ol\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:16.9pt;padding:0cm 5.4pt 0cm 5.4pt;width:85.95pt;\" width=\"115\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e0.39\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:16.9pt;padding:0cm 5.4pt 0cm 5.4pt;width:95.3pt;\" width=\"127\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e3.91\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:16.9pt;padding:0cm 5.4pt 0cm 5.4pt;width:221.4pt;\" width=\"295\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e50μL \u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;'\u003e⑨\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e+50μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:16.9pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:16.9pt;padding:0cm 5.4pt 0cm 5.4pt;width:101.2pt;\" width=\"135\" nowrap\u003e\u003cp style=\"margin-left:21.0pt;text-indent:9.0pt;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003eBlank\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:16.9pt;padding:0cm 5.4pt 0cm 5.4pt;width:85.95pt;\" width=\"115\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e0 \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:16.9pt;padding:0cm 5.4pt 0cm 5.4pt;width:95.3pt;\" width=\"127\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:16.9pt;padding:0cm 5.4pt 0cm 5.4pt;width:221.4pt;\" width=\"295\" nowrap\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;' lang=\"EN-US\"\u003e50μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp style=\"line-height:115%;margin:9.65pt 0cm .0001pt 52.05pt;\"\u003e\u003cspan style=\"letter-spacing:-.3pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e2\u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"letter-spacing:-.3pt;\"\u003e\u003cstrong\u003e. Sample Addition and Controls\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\" style=\"width:87.84%;\"\u003e\u003ctable class=\"ck-table-resized\" style=\"border-collapse:collapse;margin-left:60.75pt;margin-right:6.75pt;width:491.85pt;\" border=\"0\" cellspacing=\"0\" cellpadding=\"0\" align=\"left\" width=\"656\"\u003e\n\u003ccolgroup\u003e\n\u003ccol style=\"width:11.42%;\"\u003e\n\u003ccol style=\"width:18.94%;\"\u003e\n\u003ccol style=\"width:17.6%;\"\u003e\n\u003ccol style=\"width:20.83%;\"\u003e\n\u003ccol style=\"width:31.21%;\"\u003e\n\u003c\/colgroup\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height:30.65pt;\"\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:solid windowtext 1.0pt;height:30.65pt;padding:0cm 5.4pt 0cm 5.4pt;width:63.85pt;\" width=\"85\"\u003e\u003cp style=\"text-align:center;\"\u003e \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border-left:none;border:solid windowtext 1.0pt;height:30.65pt;padding:0cm 5.4pt 0cm 5.4pt;width:106.45pt;\" width=\"142\"\u003e\u003cp style=\"layout-grid-mode:both;text-align:center;text-autospace:ideograph-numeric ideograph-other;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;layout-grid-mode:both;'\u003e\u003cstrong\u003ePositive Control Standard Curve\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border-left:none;border:solid windowtext 1.0pt;height:30.65pt;padding:0cm 5.4pt 0cm 5.4pt;width:98.85pt;\" width=\"132\"\u003e\u003cp style=\"layout-grid-mode:both;text-align:center;text-autospace:ideograph-numeric ideograph-other;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;layout-grid-mode:both;'\u003e\u003cstrong\u003eTest Sample\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border-left:none;border:solid windowtext 1.0pt;height:30.65pt;padding:0cm 5.4pt 0cm 5.4pt;width:116.55pt;\" width=\"155\"\u003e\u003cp style=\"layout-grid-mode:both;text-align:center;text-autospace:ideograph-numeric ideograph-other;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;layout-grid-mode:both;'\u003e\u003cstrong\u003eNegative Control Well (\u003c\/strong\u003e\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;layout-grid-mode:both;' lang=\"EN-US\"\u003e\u003cstrong\u003eNC\u003c\/strong\u003e\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;layout-grid-mode:both;'\u003e\u003cstrong\u003e)\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border-left:none;border:solid windowtext 1.0pt;height:30.65pt;padding:0cm 5.4pt 0cm 5.4pt;width:106.15pt;\" width=\"142\"\u003e\u003cp style=\"layout-grid-mode:both;text-align:center;text-autospace:ideograph-numeric ideograph-other;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;layout-grid-mode:both;' lang=\"EN-US\"\u003e\u003cstrong\u003eEu\u003c\/strong\u003e\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:9.0pt;layout-grid-mode:both;'\u003e\u003cstrong\u003eControl Well\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:34.9pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:34.9pt;padding:0cm 5.4pt 0cm 5.4pt;width:63.85pt;\" rowspan=\"2\" width=\"85\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:10.0pt;' lang=\"EN-US\"\u003eStep 1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:34.9pt;padding:0cm 5.4pt 0cm 5.4pt;width:106.45pt;\" width=\"142\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:10.0pt;' lang=\"EN-US\"\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:10.0pt;'\u003eGradient Diluted\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:10.0pt;' lang=\"EN-US\"\u003eMRT-6160\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:34.9pt;padding:0cm 5.4pt 0cm 5.4pt;width:98.85pt;\" width=\"132\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:10.0pt;' lang=\"EN-US\"\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:10.0pt;'\u003eGradient Diluted Test Sample\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:34.9pt;padding:0cm 5.4pt 0cm 5.4pt;width:116.55pt;\" width=\"155\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:10.0pt;' lang=\"EN-US\"\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:10.0pt;' lang=\"EN-US\"\u003e1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:34.9pt;padding:0cm 5.4pt 0cm 5.4pt;width:106.15pt;\" rowspan=\"4\" width=\"142\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:10.0pt;' lang=\"EN-US\"\u003e15μL 1\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:10.0pt;'\u003e×\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:10.0pt;' lang=\"EN-US\"\u003e Detection buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:10.0pt;'\u003eAdd\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:10.0pt;' lang=\"EN-US\"\u003e5μL 1\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:10.0pt;'\u003e×\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:10.0pt;' lang=\"EN-US\"\u003eAnti-Tag1 Eu antibody\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:30.65pt;\"\u003e\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:30.65pt;padding:0cm 5.4pt 0cm 5.4pt;width:321.85pt;\" colspan=\"3\" width=\"429\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:10.0pt;' lang=\"EN-US\"\u003e4μL Tag1- CRBN protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr style=\"height:30.65pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;height:30.65pt;padding:0cm 5.4pt 0cm 5.4pt;width:63.85pt;\" rowspan=\"3\" width=\"85\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:10.0pt;' lang=\"EN-US\"\u003eStep 2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:30.65pt;padding:0cm 5.4pt 0cm 5.4pt;width:321.85pt;\" colspan=\"3\" width=\"429\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:10.0pt;' lang=\"EN-US\"\u003e4μL Tag2-VAV1 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:30.65pt;\"\u003e\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:30.65pt;padding:0cm 5.4pt 0cm 5.4pt;width:321.85pt;\" colspan=\"3\" width=\"429\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:10.0pt;' lang=\"EN-US\"\u003e10μL Antibody Mix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr style=\"height:31.65pt;\"\u003e\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:31.65pt;padding:0cm 5.4pt 0cm 5.4pt;width:428.0pt;\" colspan=\"4\" width=\"571\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:10.0pt;'\u003eSeal the plate with a sealing film and incubate at room temperature for\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:10.0pt;' lang=\"EN-US\"\u003e2\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:10.0pt;'\u003ehours;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\u003c\/figure\u003e\u003cp style=\"line-height:115%;margin:8.4pt 39.1pt .0001pt 71.0pt;\"\u003e \u003c\/p\u003e\n\u003cp style=\"line-height:115%;margin:8.4pt 39.1pt .0001pt 71.0pt;\"\u003e \u003c\/p\u003e\n\u003cp style=\"clear:both;\"\u003e \u003c\/p\u003e\n\u003cp style=\"line-height:115%;margin:8.4pt 39.1pt .0001pt 71.0pt;\"\u003e\u003cspan style=\"font-size:10.0pt;line-height:115%;\" lang=\"EN-US\"\u003e2.1 \u003c\/span\u003e\u003cspan style=\"font-size:10.0pt;line-height:115%;\"\u003eTest Sample\u003c\/span\u003e\u003cspan style=\"font-size:10.0pt;line-height:115%;\" lang=\"EN-US\"\u003e: 2μL\u003c\/span\u003e\u003cspan style=\"font-size:10.0pt;line-height:115%;\"\u003egradient-diluted\u003c\/span\u003e\u003cspan style=\"font-size:10.0pt;line-height:115%;\" lang=\"EN-US\"\u003etest sample\u003c\/span\u003e\u003cspan style=\"font-size:10.0pt;line-height:115%;\"\u003e, \u003c\/span\u003e\u003cspan style=\"font-size:10.0pt;line-height:115%;\" lang=\"EN-US\"\u003e4μL \u003c\/span\u003e\u003cspan style=\"font-size:9.0pt;line-height:115%;\" lang=\"EN-US\"\u003eTag1-DDB1\/CRBN protein\u003c\/span\u003e\u003cspan style=\"font-size:10.0pt;line-height:115%;\" lang=\"EN-US\"\u003eworking solution, 4μL Tag2-VAV1 protein working solution, 10μL mixed Antibody Mix, add sequentially into the 384-well shallow plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"line-height:115%;margin:8.4pt 39.1pt .0001pt 71.0pt;\"\u003e\u003cspan style=\"font-size:10.0pt;line-height:115%;\" lang=\"EN-US\"\u003e2.2  Positive Control Standard Curve: 2μL gradient-diluted MRT-6160, 4μL \u003c\/span\u003e\u003cspan style=\"font-size:9.0pt;line-height:115%;\" lang=\"EN-US\"\u003eTag1-DDB1\/CRBN protein\u003c\/span\u003e\u003cspan style=\"font-size:10.0pt;line-height:115%;\" lang=\"EN-US\"\u003eworking solution, 4μL Tag2-VAV1 protein working solution\u003c\/span\u003e\u003cspan style=\"font-size:10.0pt;line-height:115%;\"\u003e, \u003c\/span\u003e\u003cspan style=\"font-size:10.0pt;line-height:115%;\" lang=\"EN-US\"\u003e10μL Antibody Mix.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"line-height:115%;margin:8.4pt 39.1pt .0001pt 51.05pt;text-indent:20.0pt;\"\u003e\u003cspan style=\"font-size:10.0pt;line-height:115%;\" lang=\"EN-US\"\u003e2.3 \u003c\/span\u003e\u003cspan style=\"font-size:10.0pt;line-height:115%;\"\u003eNegative\u003c\/span\u003e\u003cspan style=\"font-size:10.0pt;line-height:115%;\" lang=\"EN-US\"\u003eControl\u003c\/span\u003e\u003cspan style=\"font-size:10.0pt;line-height:115%;\"\u003e Well (\u003c\/span\u003e\u003cspan style=\"font-size:10.0pt;line-height:115%;\" lang=\"EN-US\"\u003eNC\u003c\/span\u003e\u003cspan style=\"font-size:10.0pt;line-height:115%;\"\u003e): \u003c\/span\u003e\u003cspan style=\"font-size:10.0pt;line-height:115%;\" lang=\"EN-US\"\u003e2μL 1× Detection buffer replaces the\u003c\/span\u003e\u003cspan style=\"font-size:10.0pt;line\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"500T","offer_id":42332758605899,"sku":"UA086022-500T","price":2180.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":42332758573131,"sku":"UA086022-100T","price":730.0,"currency_code":"USD","in_stock":true},{"title":"2500T","offer_id":42332758638667,"sku":"UA086022-2500T","price":8175.0,"currency_code":"USD","in_stock":true},{"title":"10000T","offer_id":42332758671435,"sku":"UA086022-10000T","price":21800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_42d54618-438f-4009-9b9d-36e8edd30b58.png?v=1793423048"},{"product_id":"unione-tr-fret-human-btkcrbn-protac-binding-kit-ua086079","title":"UniOne® TR-FRET Human BTK\/CRBN PROTAC Binding Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-80℃\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eThe assay kit utilizes homogeneous time-resolved fluorescence (TR-FRET) technology to measure the interaction between the DDB1\/CRBN complex and BTK mediated by molecular glue SJF620 and test compounds. This method enables a high-throughput format for simple and rapid detection of small molecules that can mediate the interaction between the DDB1\/CRBN complex and BTK.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eAs shown, the interaction between DDB1\/CRBN and BTK is detected using a Eu-labeled anti-Tag1 antibody (TR-FRET donor) and an Accepter-labeled anti-Tag2 antibody (TR-FRET acceptor). Since molecular glue SJF620 mediates the interaction between DDB1\/CRBN and BTK, the donor antibody and acceptor antibody come into proximity, allowing the excitation of the donor antibody to trigger fluorescence resonance energy transfer (FRET) to the acceptor antibody, resulting in a specific emission signal at 665 nm. This specific signal is proportional to the extent of interaction mediated by SJF620 between DDB1\/CRBN and BTK. The homogeneous assay is simple to perform and requires no washing steps.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e                                           \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/10183402-e97a-4597-8b19-a4b26f9fc349.png\" style=\"margin-left:30px; width:531px; height:auto; vertical-align:middle;\" alt=\"\"\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003ctable border=\"1\" style=\"border-collapse:collapse;width:auto;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eComponent\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eConcentration\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e100T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e2500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e10000T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eStorage Temperature\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag1-DDB1\/CRBN protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e8\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e40\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e200\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e800\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag2- \u003c\/span\u003e\u003cspan\u003eBTK\u003c\/span\u003e\u003cspan\u003e protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e500\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2m\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eSJF620\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1m\u003c\/span\u003e\u003cspan\u003eM\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e200μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAnti-Tag1 Eu antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e250μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003em\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAnti-Tag2 \u003c\/span\u003e\u003cspan\u003eAc\u003c\/span\u003e\u003cspan\u003e antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e12.5\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003cspan\u003e0μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e200μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003em\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003cspan\u003em\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e40mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e[Experimental Procedure and Operation]\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e.\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eReagent Preparation\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e.1 \u003c\/span\u003e\u003cspan\u003eMelt all reagents at room temperature before use (equilibrate at room temperature for at least 30 min). The reaction volume for the 384-well shallow well plate is 20\u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan\u003eL (reagent volumes for the reaction system are shown in the table). Calculate the required volume for the experiment prior to preparation and prepare as needed; the following preparation is for reference only, using 500 tests as an example.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eTable 1\u003c\/span\u003e\u003cspan\u003e. \u003c\/span\u003e\u003cspan\u003eReagent Preparation\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eReagent Name\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003ePreparation\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eVolume per Detection Well (μL)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eTake\u003c\/span\u003e\u003cspan\u003e 2 mL 10\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003cspan\u003e Detection buffer 1\u003c\/span\u003e\u003cspan\u003e and add\u003c\/span\u003e\u003cspan\u003e 18 mL deionized water, di\u003c\/span\u003e\u003cspan\u003elute\u003c\/span\u003e\u003cspan\u003e to\u003c\/span\u003e\u003cspan\u003e 1×, mix\u003c\/span\u003e\u003cspan\u003e well and\u003c\/span\u003e\u003cspan\u003e set aside\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eSJF620\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eAccording to the reaction system, dilute the compound to the desired concentration using\u003c\/span\u003e\u003cspan\u003e 1× Detection buffer\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eEnsure consistent DMSO concentration across all detection wells\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag1-DDB1\/CRBN protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eTake\u003c\/span\u003e\u003cspan\u003e 40\u003c\/span\u003e\u003cspan\u003eμL \u003c\/span\u003e\u003cspan\u003eTag1-DDB1\/CRBN protein\u003c\/span\u003e\u003cspan\u003e stock solution, dilute to 2 mL with 1× Detection buffer,\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e mix well and set aside for later use.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag2- \u003c\/span\u003e\u003cspan\u003eBTK\u003c\/span\u003e\u003cspan\u003e protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eTake\u003c\/span\u003e\u003cspan\u003e 100\u003c\/span\u003e\u003cspan\u003eμL \u003c\/span\u003e\u003cspan\u003eTag2- \u003c\/span\u003e\u003cspan\u003eBTK\u003c\/span\u003e\u003cspan\u003e protein\u003c\/span\u003e\u003cspan\u003e stock solution, dilute to 2 mL with 1× Detection buffer,\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e mix well and set aside for later use.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAntibody Mix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eTake\u003c\/span\u003e\u003cspan\u003e 50μL Anti-Tag1 Eu antibody \u003c\/span\u003e\u003cspan\u003estock solution, add\u003c\/span\u003e\u003cspan\u003e 2.45 mL of 1× Detection buffer,\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e \u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003emix well;\u003c\/span\u003e\u003cspan\u003e take\u003c\/span\u003e\u003cspan\u003e 200μL Anti-Tag2 Ac antibody \u003c\/span\u003e\u003cspan\u003estock solution, add\u003c\/span\u003e\u003cspan\u003e 2.3 mL of 1× Detection buffer,\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e \u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003emix well\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e;\u003c\/span\u003e\u003cspan\u003e mix the two solutions 1:1 to prepare Antibody Mix.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan\u003e1.2 \u003c\/span\u003e\u003cspan\u003eGradient Dilution of Test Samples\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eUsing SJF620 as an example, the diluent is 1× Detection buffer. To minimize matrix effect interference, it is recommended to dilute with a solution matching the sample matrix; adjust the test samples according to actual concentrations.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eTable\u003c\/span\u003e\u003cspan\u003e2. \u003c\/span\u003e\u003cspan\u003ePositive Control Drug Gradient Dilution\u003c\/span\u003e\u003cspan\u003e (Adjust according to actual conditions)\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eSJF620 Final Concentration (nM)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eSJF620 Prepared Concentration (nM)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003ePreparation Method\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10000.00\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100000.00\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003eμL \u003c\/span\u003e\u003cspan\u003e1 mM\u003c\/span\u003e\u003cspan\u003e Stock Solution\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e18\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2500.00\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e25000.00\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL \u003c\/span\u003e\u003cspan\u003e①\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e15\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e3\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e625.00\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e6250.00\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL \u003c\/span\u003e\u003cspan\u003e②\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e15\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e156.25\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1562.50\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL \u003c\/span\u003e\u003cspan\u003e③\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e15\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e39.06\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e390.63\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5μL \u003c\/span\u003e\u003cspan\u003e④\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e15\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e6\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e9.77\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e97.66\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5μL \u003c\/span\u003e\u003cspan\u003e⑤\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e15\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e7\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2.44\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e24.41\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5μL \u003c\/span\u003e\u003cspan\u003e⑥\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e15\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e8\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e0.61\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e6.10\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5μL \u003c\/span\u003e\u003cspan\u003e⑦\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e15\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e9\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e0.15\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1.53\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5μL\u003c\/span\u003e\u003cspan\u003e⑧\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e15\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e10\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e0.04\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e0.38\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5μL \u003c\/span\u003e\u003cspan\u003e⑨\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e15\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan\u003eBlank\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e15\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003e2\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e.\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eSample Addition and Controls\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse:collapse;width:auto;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003ePositive Control Standard Curve\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eTest Samples\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eBlank\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNC\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eGradient Diluted SJF620\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eGradient Diluted Test Samples\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"4\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003eμL 1\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003cspan\u003e Detection buffer\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eAntibody Mix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4μL Tag1- CRBN protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4μL Tag2-\u003c\/span\u003e\u003cspan\u003eBTK \u003c\/span\u003e\u003cspan\u003eprotein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10μL Antibody Mix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"4\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eSeal the plate wells with a sealing film\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e,\u003c\/span\u003e\u003cspan\u003eincubate at room temperature for 2 hours;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan\u003e2.1 \u003c\/span\u003e\u003cspan\u003eTest Samples: 2μL gradient diluted test samples, 4μL Tag1-DDB1\/CRBN protein working solution, 4μL Tag2-\u003c\/span\u003e\u003cspan\u003eBTK \u003c\/span\u003e\u003cspan\u003eprotein working solution, 10μL mixed Antibody Mix (Tag2-\u003c\/span\u003e\u003cspan\u003eBTK \u003c\/span\u003e\u003cspan\u003eprotein can be mixed with Antibody Mix in proportion before addition), added sequentially into the 384-well shallow well plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.2 \u003c\/span\u003e\u003cspan\u003e Positive Control Standard Curve: 2μL gradient diluted SJF620, 4μL Tag1-DDB1\/CRBN protein working solution, 4μL Tag2-\u003c\/span\u003e\u003cspan\u003eBTK \u003c\/span\u003e\u003cspan\u003eprotein working solution, 10μL Antibody Mix.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e.3 \u003c\/span\u003e\u003cspan\u003eBlank: 2μL 1× Detection buffer replaces test samples;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.4 \u003c\/span\u003e\u003cspan\u003eNegative Control Wells (NC): 10μL 1× Detection buffer plus 10μL Antibody Mix.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAfter adding all samples, centrifuge, cover with a sealing film, and incubate at room temperature for 2 hours.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e3.\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eDetection\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eDetect using a microplate reader compatible with TR-FRET. Excitation wavelength is 320\/340nm, and emission wavelengths detected are 620nm and 665nm. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e[Result Calculation]\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1)\u003c\/span\u003e\u003cspan\u003e Calculate Signal Value (Ratio): Divide the 665nm fluorescence signal by the 620nm fluorescence signal, then multiply by 10000.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eRatio\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e=\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e(665\/620)\u003c\/span\u003e\u003cspan\u003e ×\u003c\/span\u003e\u003cspan\u003e10000\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2)\u003c\/span\u003e\u003cspan\u003e Calculate Net signal based on signal values:\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eNet signal\u003c\/span\u003e\u003cspan\u003e = (Std-\u003c\/span\u003e\u003cspan\u003eNC\u003c\/span\u003e\u003cspan\u003e)\/\u003c\/span\u003e\u003cspan\u003eNC\u003c\/span\u003e\u003cspan\u003e×100\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3)\u003c\/span\u003e\u003cspan\u003e Calculate CV (%):\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eCV (%) = Standard Deviation\/Mean Ratio × 100%\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e[Data Example]\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe following data cannot replace data obtained from experiments and is for illustration only. Results may vary depending on the microplate reader used.\u003c\/span\u003e  \u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/88659b1f-2bc4-402d-a705-621fe33d4dcb.png\" style=\"margin-left:30px; width:376px; height:auto; vertical-align:middle;\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003eNote: Recommended microplate (384-well plate, white, shallow well)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"100T","offer_id":42332763258955,"sku":"UA086079-100T","price":730.0,"currency_code":"USD","in_stock":true},{"title":"500T","offer_id":42332763291723,"sku":"UA086079-500T","price":2180.0,"currency_code":"USD","in_stock":true},{"title":"2500T","offer_id":42332763324491,"sku":"UA086079-2500T","price":8175.0,"currency_code":"USD","in_stock":true},{"title":"10000T","offer_id":42332763357259,"sku":"UA086079-10000T","price":21800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_2c75b103-29f6-4ff6-add6-0ed76493bf47.png?v=1793451851"},{"product_id":"unione-tr-fret-human-cdk2crbn-protac-binding-kit-ua086128","title":"UniOne® TR-FRET Human CDK2\/CRBN PROTAC Binding Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-80℃\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eThe kit employs homogeneous time-resolved fluorescence resonance energy transfer (TR‑FRET) technology for the quantification of molecular glue.\u003c\/span\u003e\u003cspan\u003eCDK2 Degrader7\u003c\/span\u003e\u003cspan\u003eand mediated by the compound to be detected\u003c\/span\u003e\u003cspan\u003eCRBN\u003c\/span\u003e\u003cspan\u003e(\u003c\/span\u003e\u003cspan\u003eDDB1\/CRBN complex\u003c\/span\u003e\u003cspan\u003e)\u003c\/span\u003e\u003cspan\u003ewith\u003c\/span\u003e\u003cspan\u003eCDK2(\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eCDK2\/Cyclin E1 complex)\u003c\/span\u003e\u003cspan\u003eInteraction. This method is simple and fast to implement.\u003c\/span\u003e\u003cspan\u003eland\u003c\/span\u003e\u003cspan\u003eThe assay is capable of mediating the DDB1\/CRBN complex with\u003c\/span\u003e\u003cspan\u003eCDK2\u003c\/span\u003e\u003cspan\u003eHigh-throughput screening of small-molecule interactions.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eAs\u003c\/span\u003e\u003cspan\u003eThe following figure\u003c\/span\u003e\u003cspan\u003eAs shown,\u003c\/span\u003e\u003cspan\u003eCRBN\u003c\/span\u003e\u003cspan\u003eand\u003c\/span\u003e\u003cspan\u003eCDK2\u003c\/span\u003e\u003cspan\u003eThe interaction between them was detected using an Eu-labeled anti-Tag1 antibody (TR‑FRET donor) and an Ac‑labeled anti-Tag2 antibody (TR‑FRET acceptor). Due to the molecular glue\u003c\/span\u003e\u003cspan\u003eCDK2 Degrader7\u003c\/span\u003e\u003cspan\u003eMediated CRBN and\u003c\/span\u003e\u003cspan\u003eCDK2\u003c\/span\u003e\u003cspan\u003eof the interaction, when the donor antibody approaches the receptor antibody, excitation of the donor antibody triggers fluorescence resonance energy transfer (FRET) to the receptor antibody, causing the receptor antibody to emit a signal at a wavelength of 665 nm. This specific signal is correlated with\u003c\/span\u003e\u003cspan\u003eCDK2 Degrader7\u003c\/span\u003e\u003cspan\u003eand CRBN,\u003c\/span\u003e\u003cspan\u003eCDK2\u003c\/span\u003e\u003cspan\u003ethe extent of their interaction is directly proportional. This homogeneous assay is easy to perform and requires no washing.\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/6f64731c-0d3d-416d-ab75-2119270bb798.png\" style=\"margin-left:30px; width:538px; height:auto; vertical-align:middle;\"\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003eProduct Components\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003eand Storage Conditions]\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse:collapse;width:auto;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eComponents\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eConcentration\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e100T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e2500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e10000T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eStorage Temperature\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag1-\u003c\/span\u003e\u003cspan\u003eCRBN\u003c\/span\u003e\u003cspan\u003eprotein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003e0×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag2-\u003c\/span\u003e\u003cspan\u003eCDK2\u003c\/span\u003e\u003cspan\u003eprotein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCDK2 Degrader 7\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1mM\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e25\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAnti-Tag1 Eu antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003e0×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e250\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1m\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAnti-Tag2 Ac antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e25\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e125\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e40mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan\u003eNote: After the first thaw, aliquot immediately and store at the recommended temperature. Avoid storing diluted samples or subjecting them to repeated freeze-thaw cycles.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e,\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eReagent Preparation\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e.1\u003c\/span\u003e\u003cspan\u003eBefore use, allow all reagents to thaw at room temperature (equilibrate at room temperature for at least 30 minutes). The reaction volume in a 384‑well plate is 20 µL.\u003c\/span\u003e\u003cspan\u003emu\u003c\/span\u003e\u003cspan\u003eL (reagent volumes for the reaction system are shown in the table). Calculate the required reaction volume before preparation and prepare accordingly. The following formulation is for reference only, using 500 reactions as an example.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eTable 1\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003cspan\u003eReagent Preparation\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eReagent name\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eConfiguration\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eμL per test well\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003etake\u003c\/span\u003e\u003cspan\u003e2 mL 10\u003c\/span\u003e\u003cspan\u003etimes\u003c\/span\u003e\u003cspan\u003eDetection buffer1\u003c\/span\u003e\u003cspan\u003eJoin\u003c\/span\u003e\u003cspan\u003e18 mL deionized water, dilute\u003c\/span\u003e\u003cspan\u003eExplanation\u003c\/span\u003e\u003cspan\u003eTo\u003c\/span\u003e\u003cspan\u003e1×, mixed\u003c\/span\u003e\u003cspan\u003eEvenly prepared\u003c\/span\u003e\u003cspan\u003eUse\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCDK2 Degrader7\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eAccording to the reaction system, the compound is used with\u003c\/span\u003e\u003cspan\u003eDilute the 1× detection buffer to the desired concentration.\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag1-CRBN protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003etake\u003c\/span\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e0 μL\u003c\/span\u003e\u003cspan\u003eTag1-CRBN protein\u003c\/span\u003e\u003cspan\u003eStock solution, 1× Detection buffer diluted to 2 mL,\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eMix well and set aside.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag2-\u003c\/span\u003e\u003cspan\u003eCDK2\u003c\/span\u003e\u003cspan\u003eprotein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003etake\u003c\/span\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e0 μL\u003c\/span\u003e\u003cspan\u003eTag2-\u003c\/span\u003e\u003cspan\u003eCDK2\u003c\/span\u003e\u003cspan\u003eprotein\u003c\/span\u003e\u003cspan\u003eStock solution, diluted with 1× Detection buffer to 2 mL,\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003emix well and set aside.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAntibody Mix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:justify\"\u003e\u003cspan style=\"font-weight:bold\"\u003eTake\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e50\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eμL Anti-Tag1 Eu antibody\u003c\/span\u003e\u003cspan\u003estock solution, add\u003c\/span\u003e\u003cspan\u003e1× Detection buffer 2.4\u003c\/span\u003e\u003cspan\u003e50\u003c\/span\u003e\u003cspan\u003emL,\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e \u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003emix well;\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003etake\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e25\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eμL Anti-Tag2 Ac antibody\u003c\/span\u003e\u003cspan\u003estock solution, add\u003c\/span\u003e\u003cspan\u003e1× Detection buffer 2.\u003c\/span\u003e\u003cspan\u003e475\u003c\/span\u003e\u003cspan\u003emL,\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e \u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003emix well\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e;\u003c\/span\u003e\u003cspan\u003ethe two\u003c\/span\u003e\u003cspan\u003eare mixed 1:1 to form the Antibody Mix.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan\u003e1.2\u003c\/span\u003e\u003cspan\u003esample to be tested\u003c\/span\u003e\u003cspan\u003egradient dilution\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eusing\u003c\/span\u003e\u003cspan\u003eCDK2 Degrader7\u003c\/span\u003e\u003cspan\u003eas an example, the diluent is 1× Detection buffer,\u003c\/span\u003e\u003cspan\u003eto reduce the impact of matrix effect interference, it is recommended to use a solution with the same matrix as the sample being tested; and\u003c\/span\u003e\u003cspan\u003ethe sample to be tested should be adjusted according to its actual concentration.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eTable\u003c\/span\u003e\u003cspan\u003e2.\u003c\/span\u003e\u003cspan\u003eCDK2 Degrader7\u003c\/span\u003e\u003cspan\u003egradient dilution\u003c\/span\u003e\u003cspan\u003e(adjusted based on actual conditions)\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eCDK2 Degrader7\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003efinal concentration (nM)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eCDK2 Degrader7\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003ePreparation concentration (nM)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003ePreparation method\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e①\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-style:italic;text-decoration:underline;color:#000000\"\u003e1000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-style:italic;text-decoration:underline;color:#000000\"\u003e10000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003cspan\u003e1m\u003c\/span\u003e\u003cspan\u003eM\u003c\/span\u003e\u003cspan\u003eCDK2 Degrader7\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e99\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e②\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-style:italic;text-decoration:underline;color:#000000\"\u003e333.3333\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-style:italic;text-decoration:underline;color:#000000\"\u003e3333.333\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e0μL\u003c\/span\u003e\u003cspan\u003e①\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e40\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e③\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-style:italic;text-decoration:underline;color:#000000\"\u003e111.1111\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-style:italic;text-decoration:underline;color:#000000\"\u003e1111.111\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e0μL\u003c\/span\u003e\u003cspan\u003e②\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e40\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e④\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-style:italic;text-decoration:underline;color:#000000\"\u003e37.0370\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-style:italic;text-decoration:underline;color:#000000\"\u003e370.370\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e0μL\u003c\/span\u003e\u003cspan\u003e③\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e40\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑤\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-style:italic;text-decoration:underline;color:#000000\"\u003e12.3457\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-style:italic;text-decoration:underline;color:#000000\"\u003e123.457\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e0μL\u003c\/span\u003e\u003cspan\u003e④\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e40\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑥\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-style:italic;text-decoration:underline;color:#000000\"\u003e4.1152\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-style:italic;text-decoration:underline;color:#000000\"\u003e41.152\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e0μL\u003c\/span\u003e\u003cspan\u003e⑤\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e40\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑦\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-style:italic;text-decoration:underline;color:#000000\"\u003e1.3717\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-style:italic;text-decoration:underline;color:#000000\"\u003e13.717\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e0μL\u003c\/span\u003e\u003cspan\u003e⑥\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e40\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑧\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-style:italic;text-decoration:underline;color:#000000\"\u003e0.4572\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-style:italic;text-decoration:underline;color:#000000\"\u003e4.572\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e0μL\u003c\/span\u003e\u003cspan\u003e⑦\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e40\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑨\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-style:italic;text-decoration:underline;color:#000000\"\u003e0.1524\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-style:italic;text-decoration:underline;color:#000000\"\u003e1.524\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e0μL\u003c\/span\u003e\u003cspan\u003e⑧\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e40\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eBlank\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-style:italic;text-decoration:underline;color:#000000\"\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-style:italic;text-decoration:underline;color:#000000\"\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003cspan\u003e0μL of 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003e2\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e,\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eSample addition and controls\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003ePositive drug standard curve\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eSample to be tested\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eBlank\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eControl well\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNC\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eGradient dilution\u003c\/span\u003e\u003cspan\u003eCDK2 Degrader7\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eGradient-diluted sample to be tested\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"4\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e0\u003c\/span\u003e\u003cspan\u003eμL 1\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd\u003c\/span\u003e\u003cspan\u003e10μL Antibody Mix\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4μL\u003c\/span\u003e\u003cspan\u003eTag1-CRBN protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4μL\u003c\/span\u003e\u003cspan\u003eTag2-\u003c\/span\u003e\u003cspan\u003eCDK2\u003c\/span\u003e\u003cspan\u003eprotein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10μL Antibody Mix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"4\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eSeal the plate wells with a sealing film.\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e,\u003c\/span\u003e\u003cspan\u003eIncubate at room temperature for 2 hours;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan\u003e2.1\u003c\/span\u003e\u003cspan\u003eSample to be tested\u003c\/span\u003e\u003cspan\u003e: 4 μL\u003c\/span\u003e\u003cspan\u003eTag1-CRBN protein\u003c\/span\u003e\u003cspan\u003eworking solution, 4 μL\u003c\/span\u003e\u003cspan\u003eTag2-\u003c\/span\u003e\u003cspan\u003eCDK2\u003c\/span\u003e\u003cspan\u003eprotein\u003c\/span\u003e\u003cspan\u003eworking solution, 2μL\u003c\/span\u003e\u003cspan\u003egradient dilution\u003c\/span\u003e\u003cspan\u003esample to be tested\u003c\/span\u003e\u003cspan\u003e,\u003c\/span\u003e\u003cspan\u003e10μL of mixed Antibody Mix, added sequentially into the 384-well plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.2\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003epositive drug standard curve: 4μL\u003c\/span\u003e\u003cspan\u003eTag1-CRBN protein\u003c\/span\u003e\u003cspan\u003eworking solution, 4μL\u003c\/span\u003e\u003cspan\u003eTag2-\u003c\/span\u003e\u003cspan\u003eCDK2\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eprotein\u003c\/span\u003e\u003cspan\u003eworking solution\u003c\/span\u003e\u003cspan\u003e,\u003c\/span\u003e\u003cspan\u003e2μL gradient-diluted\u003c\/span\u003e\u003cspan\u003eCDK2 Degrader7\u003c\/span\u003e\u003cspan\u003e, 10μL Antibody Mix.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e.3\u003c\/span\u003e\u003cspan\u003eblank\u003c\/span\u003e\u003cspan\u003econtrol\u003c\/span\u003e\u003cspan\u003ewell\u003c\/span\u003e\u003cspan\u003e: 2μL of 1× Detection buffer instead\u003c\/span\u003e\u003cspan\u003eof the sample to be tested\u003c\/span\u003e\u003cspan\u003e;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.4\u003c\/span\u003e\u003cspan\u003eNC\u003c\/span\u003e\u003cspan\u003e: 1\u003c\/span\u003e\u003cspan\u003e0\u003c\/span\u003e\u003cspan\u003eμL 1\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003cspan\u003eadd\u003c\/span\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eAntibody Mix\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAfter adding all samples, centrifuge, seal with a plate cover, and incubate at room temperature for 2 hours.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e3,\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eDetection\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eDetect on an ELISA reader compatible with TR-FRET.\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003cspan\u003eThe excitation light is 320\/340 nm, and the emission wavelengths are detected at 620 nm and 665 nm.\u003c\/span\u003e\u003cspan\u003eEmission\u003c\/span\u003e\u003cspan\u003ewavelengths.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e[Result Calculation]\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1)\u003c\/span\u003e\u003cspan\u003eCalculate the signal value\u003c\/span\u003e\u003cspan\u003e(Ratio)\u003c\/span\u003e\u003cspan\u003e: Multiply the 665 nm fluorescence signal by the 620 nm fluorescence signal, then multiply by 10,000\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eRatio\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e=\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e(665\/620)\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003cspan\u003e10,000\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2)\u003c\/span\u003e\u003cspan\u003eCalculate based on the signal value\u003c\/span\u003e\u003cspan\u003eNet signal\u003c\/span\u003e\u003cspan\u003e:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eNet signal\u003c\/span\u003e\u003cspan\u003e= (Std-\u003c\/span\u003e\u003cspan\u003eNC\u003c\/span\u003e\u003cspan\u003e)\/\u003c\/span\u003e\u003cspan\u003eNC\u003c\/span\u003e\u003cspan\u003e×100\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3)\u003c\/span\u003e\u003cspan\u003eCalculate CV (%)\u003c\/span\u003e\u003cspan\u003e:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eCV (%) = Standard Deviation \/ Mean Ratio × 100%\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e[[Data Example]\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe following data cannot replace the data obtained in experiments; it is only for illustration, and results may\u003c\/span\u003e\u003cspan\u003evary depending on the plate reader\u003c\/span\u003e\u003cspan\u003eand\u003c\/span\u003e\u003cspan\u003eequipment.\u003c\/span\u003e  \u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/951e80f9-7316-42ea-9bf5-57e4b518838f.png\" style=\"margin-left:30px; width:424px; height:auto; vertical-align:middle;\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/7473c47a-7f85-4425-8b40-65e58f9f00ed.png\" style=\"margin-left:30px; width:277px; height:auto; vertical-align:middle;\"\u003e\u003c\/p\u003e\n\u003cspan\u003e \u003c\/span\u003e\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/b3ffcf38-ad79-4f80-8abb-8fbd6134ac93.png\" style=\"margin-left:30px; width:343px; height:auto; vertical-align:middle;\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003eNote: Recommended\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eMicroplate (384-well plate, white, shallow wells)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"100T","offer_id":42332773613643,"sku":"UA086128-100T","price":730.0,"currency_code":"USD","in_stock":true},{"title":"500T","offer_id":42332773646411,"sku":"UA086128-500T","price":2180.0,"currency_code":"USD","in_stock":true},{"title":"2500T","offer_id":42332773679179,"sku":"UA086128-2500T","price":8175.0,"currency_code":"USD","in_stock":true},{"title":"10000T","offer_id":42332773711947,"sku":"UA086128-10000T","price":21800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_a3ea4df7-5597-4856-9810-2adc9a6d6ed3.png?v=1793487710"},{"product_id":"unione-tr-fret-human-itkcrbn-protac-binding-kit-ua086139","title":"UniOne® TR-FRET Human ITK\/CRBN PROTAC Binding Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-80℃\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e【\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003eASSAY PRINCIPLE\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e】\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eThe kit is based on homogeneous time-resolved fluorescence technology (TR-FRET) technology，which is used to assess the molecular-mediated interaction between the DDB1\/CRBN complex and\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eITK. This method provides a high-throughput approach to detect the small molecules capable of mediating the interaction between the DDB1\/CRBN complex and\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eITK simply and rapidly.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eAs shown in the figure below, the interaction between DDB1\/CRBN and\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eITK was detected by an Eu-labeled anti-Tag1 antibody (TR-FRET donor) and an Ac-labeled anti-Tag2 antibody (TR-FRET receptor). Since the molecular adhesive \u003c\/span\u003e\u003cspan\u003eBSJ-05-037\u003c\/span\u003e\u003cspan\u003e mediates the interaction between DDB1\/CRBN and\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eITK, the donor antibody is in close proximity to the receptor antibody. Excitation of the donor antibody triggers fluorescence resonance energy transfer (FRET) to the receptor antibody, resulting in specific emission of a signal at a wavelength of 665 nm from the receptor antibody. This specific signal is proportional to the degree of interaction between \u003c\/span\u003e\u003cspan\u003eBSJ-05-037\u003c\/span\u003e\u003cspan\u003e, DDB1\/CRBN and\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eITK. This homogeneous assay is simple to perform and requires no washing steps.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/78ad499b-bc46-46a9-8fa6-5b80d04eeace.png\" style=\"margin-left:30px; width:618px; height:auto; vertical-align:middle;\" alt=\"\"\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e【Composition and Storage Conditions】\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse:collapse;width:auto;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eComposition \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003econcentration\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e100T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e2500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e10000T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eStorage \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag1-CRBN protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e00\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag2-\u003c\/span\u003e\u003cspan\u003eITK \u003c\/span\u003e\u003cspan\u003eprotein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e7\u003c\/span\u003e\u003cspan\u003e8\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e6\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e26\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e130\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e520\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eBSJ-05-037\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e500μM\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e200μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAnti-Tag1 Eu antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003e0×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e250\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e000\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAnti-Tag2 Ac antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e2.5\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e40\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e200\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e000\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003cspan\u003emL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e40mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan\u003eNote: Aliquot into appropriate volumes, store at the recommended temperature and avoid repeated freeze-thaw cycle.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e[Process and Operations]\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e, Reagent Preparation\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e.1\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eCompositions\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eequilibration\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eBefore use, dissolve all reagents at room temperature (allow at least 30 minutes for temperature equilibration). For the 384-well plate assay, use 20 μL of reaction mixture (the reagent quantities are listed in the table below). Calculate the required volume prior to preparation accordingly. The following preparation steps are provided for reference only, using a 500-well plate as an example.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eTable 1. Reagent Preparation\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eComposition\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eConfiguration\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eμL\/well\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDetection\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003ebuffer\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan\u003eAdd 2 mL of 10× Detection buffer to 18 mL of deionized water, dilute to 1× Detection , and mix thoroughly for later use.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag1-CRBN protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan\u003eTake\u003c\/span\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e0 μL of the\u003c\/span\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e00× Tag1-CRBN protein stock solution, add 1.9\u003c\/span\u003e\u003cspan\u003e8\u003c\/span\u003e\u003cspan\u003emL of 1× Detection buffer to dilute it to 1× concentration, and mix thoroughly for later use.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag2-\u003c\/span\u003e\u003cspan\u003eITK\u003c\/span\u003e\u003cspan\u003eprotein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan\u003eTake\u003c\/span\u003e\u003cspan\u003e26\u003c\/span\u003e\u003cspan\u003eμL of\u003c\/span\u003e\u003cspan\u003e78\u003c\/span\u003e\u003cspan\u003e× Tag2-\u003c\/span\u003e\u003cspan\u003eITK\u003c\/span\u003e\u003cspan\u003eprotein, add it to\u003c\/span\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003cspan\u003e002\u003c\/span\u003e\u003cspan\u003emL of 1× Detection buffer to dilute to 1× concentration, and mix thoroughly for later use.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAntibody\u003c\/span\u003e\u003cspan\u003eMix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTake\u003c\/span\u003e\u003cspan\u003e50\u003c\/span\u003e\u003cspan\u003eμL of the\u003c\/span\u003e\u003cspan\u003e50\u003c\/span\u003e\u003cspan\u003e× Eu-anti-Tag1 stock solution, add 24\u003c\/span\u003e\u003cspan\u003e50\u003c\/span\u003e\u003cspan\u003eμL of 1× Detection buffer to dilute to 2.5 mL, and mix thoroughly; take\u003c\/span\u003e\u003cspan\u003e200\u003c\/span\u003e\u003cspan\u003eμL of the\u003c\/span\u003e\u003cspan\u003e12.5\u003c\/span\u003e\u003cspan\u003e× Ac-anti-Tag2 stock solution, add 2\u003c\/span\u003e\u003cspan\u003e300\u003c\/span\u003e\u003cspan\u003eμL of 1× Detection buffer to dilute to 2.\u003c\/span\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003emL, and mix thoroughly; combine the two solutions in a 1:1 ratio to obtain the\u003c\/span\u003e\u003cspan\u003eAntibody\u003c\/span\u003e\u003cspan\u003eMix.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003e1.2\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eGradient dilution of the sample\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eBSJ-05-037\u003c\/span\u003e\u003cspan\u003eas an example, the dilution solution is 1× Detection buffer. To minimize interference from matrix effects, it is recommended to use a solution with the same matrix composition as the sample being tested; additionally, the sample concentration should be adjusted according to its actual situation.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eTable\u003c\/span\u003e\u003cspan\u003e2.\u003c\/span\u003e\u003cspan\u003eBSJ-05-037\u003c\/span\u003e\u003cspan\u003egradient dilution(Adjust according to the actual situation)\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eBSJ-05-037\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003efinal concentration (nM)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eBSJ-05-037\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003epreparation concentration (nM)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eDilution\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e①\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2μL 5\u003c\/span\u003e\u003cspan\u003e00 μ\u003c\/span\u003e\u003cspan\u003eM\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eBSJ-05-037\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e9\u003c\/span\u003e\u003cspan\u003e8μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e②\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e333.33\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3333.33\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003e0μL\u003c\/span\u003e\u003cspan\u003e①\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e60\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e③\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e111.11\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1111.11\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003e0μL\u003c\/span\u003e\u003cspan\u003e②\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e60\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e④\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e37.04\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e370.37\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003e0μL\u003c\/span\u003e\u003cspan\u003e③\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e60\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑤\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e12.35\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e123.46\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003e0μL\u003c\/span\u003e\u003cspan\u003e④\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e60\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑥\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4.12\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e41.15\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003e0μL\u003c\/span\u003e\u003cspan\u003e⑤\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e60\u003c\/span\u003e\u003cspan\u003eμL of 1× Detection Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑦\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1.37\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e13.72\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003e0μL\u003c\/span\u003e\u003cspan\u003e⑥\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e60\u003c\/span\u003e\u003cspan\u003eμL of 1× Detection Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑧\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e0.46\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003cspan\u003e57\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003e0μL\u003c\/span\u003e\u003cspan\u003e⑦\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e60\u003c\/span\u003e\u003cspan\u003eμL of 1× Detection Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑨\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e0.15\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1.52\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003e0μL\u003c\/span\u003e\u003cspan\u003e⑧\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e60\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑩\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e0.05\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e0.51\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003e0μL\u003c\/span\u003e\u003cspan\u003e⑨\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e60\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eBlank\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e6\u003c\/span\u003e\u003cspan\u003e0μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003e2\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e, Sample and control\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003e2.1\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eSam\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eple:\u003c\/span\u003e\u003cspan\u003eAdd 4 μL of Tag1-CRBN protein working solution, 4 μL of Tag2-\u003c\/span\u003e\u003cspan\u003eITK\u003c\/span\u003e\u003cspan\u003eprotein working solution, 2 μL of the gradient-diluted sample , and 10 μL of the mixed Antibody Mix sequentially into the 384-well plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003e2.2\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eBSJ-05-037\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e:\u003c\/span\u003e\u003cspan\u003e4 μL of Tag1-CRBN protein working solution, 4 μL of Tag2-\u003c\/span\u003e\u003cspan\u003eITK\u003c\/span\u003e\u003cspan\u003eprotein working solution, 2 μL of gradient-diluted\u003c\/span\u003e\u003cspan\u003eBSJ-05-037\u003c\/span\u003e\u003cspan\u003e, and 10 μL of the mixed Antibody Mix sequentially into the 384-well plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003e2.3 Blank:\u003c\/span\u003e\u003cspan\u003e4 μL of Tag1-CRBN protein working solution, 4 μL of Tag2-\u003c\/span\u003e\u003cspan\u003eITK\u003c\/span\u003e\u003cspan\u003eprotein working solution, 2 μL of\u003c\/span\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003cspan\u003e, and 10 μL of the mixed Antibody Mix sequentially into the 384-well plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003e2.3\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eNegative\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e \u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003econtrol\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e:\u003c\/span\u003e\u003cspan\u003e10μL Detection buffer\u003c\/span\u003e\u003cspan\u003e,\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eand 10 μL of the mixed Antibody Mix sequentially into the 384-well plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAfter adding all samples, centrifuge, seal with a cover membrane, and incubate at room temperature for 2 hours.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eTable 3.\u003c\/span\u003e\u003cspan\u003eSample and control spotting process\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eDEG-35\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eSample\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eBlank\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNegative\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e \u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003econtrol(\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eNC)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003egradient-diluted\u003c\/span\u003e\u003cspan\u003eBSJ-05-037\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003egradient-diluted sample\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"4\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e0\u003c\/span\u003e\u003cspan\u003eμL 1\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eand 10μL Antibody Mix\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4μL Tag1-CRBN protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4μL Tag2-\u003c\/span\u003e\u003cspan\u003eITK\u003c\/span\u003e\u003cspan\u003eprotein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10 μL Antibody Mix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"4\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eSeal the plate wells with a sealing film and incubate at room temperature for 2 hours.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e3,\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eRead Data\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe detection was performed on a TR-FRET microplate reader. The excitation wavelength was 320\/340 nm, and the emission wavelengths at 620 nm and 665 nm were detected.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e[DATA REDUCTION]\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1)\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eRatio\u003c\/span\u003e\u003cspan\u003e:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eRatio\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e=\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e(665\/620)\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003cspan\u003e10000\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2)\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eNet signal\u003c\/span\u003e\u003cspan\u003e:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eNet signal\u003c\/span\u003e\u003cspan\u003e= (Std-\u003c\/span\u003e\u003cspan\u003eNC\u003c\/span\u003e\u003cspan\u003e)\/\u003c\/span\u003e\u003cspan\u003eNC\u003c\/span\u003e\u003cspan\u003e×100\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eStd: the ratio of sample and control\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eNC: the ratio of\u003c\/span\u003e\u003cspan\u003enegative\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003econtrol\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3)\u003c\/span\u003e\u003cspan\u003eCV (%)\u003c\/span\u003e\u003cspan\u003e:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eCV (%) = Standard Deviation\/Mean Ratio × 100%\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e[\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003eData Example\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e]\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe following data cannot replace experimental results obtained in experiments;\u003c\/span\u003e\u003cspan\u003ethey\u003c\/span\u003e\u003cspan\u003eare provided solely for illustrative purposes, and outcomes may vary depending on the plate reader used.\u003c\/span\u003e \u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/af810ad9-f909-4552-8bb2-d847566985df.png\" style=\"margin-left:30px; width:274px; height:auto; vertical-align:middle;\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003eNote: Recommended microplate (384-well plate, white, shallow wells).\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"100T","offer_id":42989602275403,"sku":"UA086139-100T","price":730.0,"currency_code":"USD","in_stock":true},{"title":"500T","offer_id":42989602308171,"sku":"UA086139-500T","price":2180.0,"currency_code":"USD","in_stock":true},{"title":"2500T","offer_id":42989602340939,"sku":"UA086139-2500T","price":8175.0,"currency_code":"USD","in_stock":true},{"title":"10000T","offer_id":42989602373707,"sku":"UA086139-10000T","price":21800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_e942791d-c3e5-4f58-a04e-bf8c5b042ccc.png?v=1793516543"},{"product_id":"unione-tr-fret-human-cdk4crbn-protac-binding-kit-ua086136","title":"UniOne® TR-FRET Human CDK4\/CRBN PROTAC Binding Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-80℃\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e【\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003eASSAY PRINCIPLE\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e】\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eThe kit is based on homogeneous time-resolved fluorescence technology (TR-FRET) technology，which is used to assess the molecular-mediated interaction between the DDB1\/CRBN complex and CDK4\/Cyclin D3 complex (CDK4). This method provides a high-throughput approach to detect the small molecules capable of mediating the interaction between the DDB1\/CRBN complex and CDK4\/Cyclin D3 complex simply and rapidly .\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eAs shown in the figure below, the interaction between DDB1\/CRBN and \u003c\/span\u003e\u003cspan\u003eCDK4\/Cyclin D3\u003c\/span\u003e\u003cspan\u003e was detected by an Eu-labeled anti-Tag1 antibody (TR-FRET donor) and an Ac-labeled anti-Tag2 antibody (TR-FRET receptor). Since the molecular adhesive BSJ-03-204 mediates the interaction between DDB1\/CRBN and CDK4\/Cyclin D3, the donor antibody is in close proximity to the receptor antibody. Excitation of the donor antibody triggers fluorescence resonance energy transfer (FRET) to the receptor antibody, resulting in specific emission of a signal at a wavelength of 665 nm from the receptor antibody. This specific signal is proportional to the degree of interaction between BSJ-03-204, DDB1\/CRBN and CDK4\/Cyclin D3. This homogeneous assay is simple to perform and requires no washing steps.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e      \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/b249b5ec-b24b-4781-bd56-8ad929411578.png\" style=\"margin-left:30px; width:526px; height:auto; vertical-align:middle;\" alt=\"\"\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e【Composition and Storage Conditions】\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse:collapse;width:auto;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eComposition \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003econcentration\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e100T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e2500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e10000T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eStorage \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag1-CRBN protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag2-CDK4 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e126×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e16μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e80μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e320μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eBSJ-03-204\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1 mM\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e200μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAnti-Tag1 Eu antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e250μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1000μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAnti-Tag2 Ac antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e25μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e125μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e500μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e15mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e75mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e300mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan\u003eNote: Aliquot into appropriate volumes, store at the recommended temperature and avoid repeated freeze-thaw cycle.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e[Process and Operations]\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e, Reagent Preparation\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e.1\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eCompositions\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eequilibration\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eBefore use, dissolve all reagents at room temperature (allow at least 30 minutes for temperature equilibration). For the 384-well plate assay, use 20 μL of reaction mixture (the reagent quantities are listed in the table below). Calculate the required volume prior to preparation accordingly. The following preparation steps are provided for reference only, using a 500-well plate as an example.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eTable 1. Reagent Preparation\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eComposition\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eConfiguration\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eμL\/well\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag1-CRBN protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan\u003eTake 20 μL of the 100× Tag1-CRBN protein stock solution, add 1.98 mL of Detection buffer to dilute it to 1× concentration, and mix thoroughly for later use.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag2-CDK4 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan\u003eTake 16 μL of 126×\u003c\/span\u003e\u003cspan\u003eTag2-CDK4 protein\u003c\/span\u003e\u003cspan\u003estock solution, add it to 2 mL of Detection buffer to dilute to 1× concentration, and mix thoroughly for later use.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAntibody\u003c\/span\u003e\u003cspan\u003eMix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTake 50 μL of the 50×\u003c\/span\u003e\u003cspan\u003eAnti-Tag1 Eu antibody\u003c\/span\u003e\u003cspan\u003estock solution, add 2450 μL of Detection buffer to dilute to 2.5 mL, and mix thoroughly; take 25 μL of the 100×\u003c\/span\u003e\u003cspan\u003eAnti-Tag2 Ac antibody\u003c\/span\u003e\u003cspan\u003estock solution, add 2475 μL of Detection buffer to dilute to 2.5 mL, and mix thoroughly; combine the two solutions in a 1:1 ratio to obtain the\u003c\/span\u003e\u003cspan\u003eAntibody\u003c\/span\u003e\u003cspan\u003eMix.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003e1.2\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eGradient dilution of the sample\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eBSJ-03-204 as an example, the dilution solution is Detection buffer. To minimize interference from matrix effects, it is recommended to use a solution with the same matrix composition as the sample being tested; additionally, the sample concentration should be adjusted according to its actual situation.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eTable\u003c\/span\u003e\u003cspan\u003e2.\u003c\/span\u003e\u003cspan\u003eBSJ-03-204 gradient dilution (Adjust according to the actual situation)\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eBSJ-03-204 final concentration (nM)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eBSJ-03-204 preparation concentration (nM)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eDilution\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e①\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003cspan\u003e1m\u003c\/span\u003e\u003cspan\u003eM\u003c\/span\u003e\u003cspan\u003eBSJ-03-204\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e98\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e②\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003e0μL\u003c\/span\u003e\u003cspan\u003e①\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e30\u003c\/span\u003e\u003cspan\u003eμL Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e③\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e500\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003e0μL\u003c\/span\u003e\u003cspan\u003e②\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e30\u003c\/span\u003e\u003cspan\u003eμL Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e④\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e250\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2500\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003e0μL\u003c\/span\u003e\u003cspan\u003e③\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e30\u003c\/span\u003e\u003cspan\u003eμL Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑤\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e125\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1250\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003e0μL\u003c\/span\u003e\u003cspan\u003e④\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e30\u003c\/span\u003e\u003cspan\u003eμL Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑥\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e125\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e625\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003e0μL\u003c\/span\u003e\u003cspan\u003e⑤\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e30\u003c\/span\u003e\u003cspan\u003eμL Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑦\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e31.25\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e312.5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003e0μL\u003c\/span\u003e\u003cspan\u003e⑥\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e30\u003c\/span\u003e\u003cspan\u003eμL Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑧\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e15.625\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e156.25\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003e0μL\u003c\/span\u003e\u003cspan\u003e⑦\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e30\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑨\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e7.8125\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e78.125\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003e0μL\u003c\/span\u003e\u003cspan\u003e⑧\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e30\u003c\/span\u003e\u003cspan\u003eμL Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑩\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3.90625\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e39.0625\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003e0μL\u003c\/span\u003e\u003cspan\u003e⑨\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e30\u003c\/span\u003e\u003cspan\u003eμL Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eBlank\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003e0μL Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003e2\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e,Sample and control\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003e2.1\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eSam\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eple:\u003c\/span\u003e\u003cspan\u003eAdd 4 μL of Tag1-CRBN protein working solution, 4 μL of\u003c\/span\u003e\u003cspan\u003eTag2-\u003c\/span\u003e\u003cspan\u003eCDK4\u003c\/span\u003e\u003cspan\u003eprotein\u003c\/span\u003e\u003cspan\u003eworking solution, 2 μL of the gradient-diluted sample , and 10 μL of the mixed Antibody Mix sequentially into the 384-well plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003e2.2 BSJ-03-204:\u003c\/span\u003e\u003cspan\u003e4 μL of Tag1-CRBN protein working solution, 4 μL of\u003c\/span\u003e\u003cspan\u003eTag2-\u003c\/span\u003e\u003cspan\u003eCDK4\u003c\/span\u003e\u003cspan\u003eprotein\u003c\/span\u003e\u003cspan\u003eworking solution, 2 μL of gradient-diluted BSJ-03-204, and 10 μL of the mixed Antibody Mix sequentially into the 384-well plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003e2.3 Blank:\u003c\/span\u003e\u003cspan\u003e4 μL of Tag1-CRBN protein working solution, 4 μL of\u003c\/span\u003e\u003cspan\u003eTag2-\u003c\/span\u003e\u003cspan\u003eCDK4\u003c\/span\u003e\u003cspan\u003eprotein\u003c\/span\u003e\u003cspan\u003eworking solution, 2 μL of\u003c\/span\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003cspan\u003e, and 10 μL of the mixed Antibody Mix sequentially into the 384-well plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003e2.3\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eNegative\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e \u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003econtrol\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e:\u003c\/span\u003e\u003cspan\u003e10μL Detection buffer\u003c\/span\u003e\u003cspan\u003e,\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eand 10 μL of the mixed Antibody Mix sequentially into the 384-well plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAfter adding all samples, centrifuge, seal with a cover membrane, and incubate at room temperature for 2 hours.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eTable 3.\u003c\/span\u003e\u003cspan\u003eSample and control spotting process\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eBSJ-03-204\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eSample\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eBlank\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNegative\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e \u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003econtrol(\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eNC)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003egradient-diluted BSJ-03-204\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003egradient-diluted sample\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"4\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e0\u003c\/span\u003e\u003cspan\u003eμL Detection buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eand 10μL Antibody Mix\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4μL Tag1-CRBN protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4μL Tag2-CDK4 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10μL Antibody Mix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"4\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eSeal the plate wells with a sealing film and incubate at room temperature more than 2 hours.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e3,\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eRead Data\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe detection was performed on a TR-FRET microplate reader. The excitation wavelength was 320\/340 nm, and the emission wavelengths at 620 nm and 665 nm were detected.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e[DATA REDUCTION ]\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/d3873be8-5060-4810-b64b-ddbeb870361c.png\" style=\"margin-left:30px; width:295px; height:auto; vertical-align:middle;\"\u003e\u003c\/p\u003e\n\u003cspan\u003e1)\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eRatio\u003c\/span\u003e\u003cspan\u003e:\u003c\/span\u003e\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eRatio\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e=\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e(665\/620)\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003cspan\u003e10000\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2)\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eNet signal\u003c\/span\u003e\u003cspan\u003e:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eNet signal\u003c\/span\u003e\u003cspan\u003e= (Std-\u003c\/span\u003e\u003cspan\u003eNC\u003c\/span\u003e\u003cspan\u003e)\/\u003c\/span\u003e\u003cspan\u003eNC\u003c\/span\u003e\u003cspan\u003e×100\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eStd:the ratio of sample and control\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eNC:   the ratio of\u003c\/span\u003e\u003cspan\u003enegative\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003econtrol\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3)\u003c\/span\u003e\u003cspan\u003eCV(%)\u003c\/span\u003e\u003cspan\u003e:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eCV(%)= Standard Deviation\/Mean Ratio × 100%\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e[\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003eData Example\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e]\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe following data cannot replace experimental results obtained in experiments;\u003c\/span\u003e\u003cspan\u003ethey\u003c\/span\u003e\u003cspan\u003eare provided solely for illustrative purposes, and outcomes may vary depending on the plate reader used.\u003c\/span\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003eNote: Recommended microplate (384-well plate, white, shallow wells).\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"100T","offer_id":42989602570315,"sku":"UA086136-100T","price":730.0,"currency_code":"USD","in_stock":true},{"title":"500T","offer_id":42989602603083,"sku":"UA086136-500T","price":2180.0,"currency_code":"USD","in_stock":true},{"title":"2500T","offer_id":42989602635851,"sku":"UA086136-2500T","price":8175.0,"currency_code":"USD","in_stock":true},{"title":"10000T","offer_id":42989602668619,"sku":"UA086136-10000T","price":21800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_71c496b5-0318-4c6b-ad64-7e461f85fcb9.png?v=1793509214"},{"product_id":"unione-tr-fret-human-cdk6crbn-protac-binding-kit-ua086137","title":"UniOne® TR-FRET Human CDK6\/CRBN PROTAC Binding Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-80℃\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e【\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003eASSAY PRINCIPLE\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e】\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eThe kit is based on homogeneous time-resolved fluorescence technology (TR-FRET) technology，which is used to assess the molecular-mediated interaction between the DDB1\/CRBN complex and CDK6\/Cyclin D3 complex (CDK6). This method provides a high-throughput approach to detect the small molecules capable of mediating the interaction between the DDB1\/CRBN complex and CDK6\/Cyclin D3 complex simply and rapidly .\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eAs shown in the figure below, the interaction between DDB1\/CRBN and \u003c\/span\u003e\u003cspan\u003eCDK6\/Cyclin D3\u003c\/span\u003e\u003cspan\u003e was detected by an Eu-labeled anti-Tag1 antibody (TR-FRET donor) and an Ac-labeled anti-Tag2 antibody (TR-FRET receptor). Since the molecular adhesive BSJ-03-204 mediates the interaction between DDB1\/CRBN and CDK6\/Cyclin D3, the donor antibody is in close proximity to the receptor antibody. Excitation of the donor antibody triggers fluorescence resonance energy transfer (FRET) to the receptor antibody, resulting in specific emission of a signal at a wavelength of 665 nm from the receptor antibody. This specific signal is proportional to the degree of interaction between BSJ-03-204, DDB1\/CRBN and CDK6\/Cyclin D3. This homogeneous assay is simple to perform and requires no washing steps.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e      \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/1ca0102e-cd74-4e93-b744-78587040179a.png\" style=\"margin-left:30px; width:537px; height:auto; vertical-align:middle;\" alt=\"\"\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e【Composition and Storage Conditions】\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse:collapse;width:auto;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eComposition \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003econcentration\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e100T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e2500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e10000T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eStorage \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag1-CRBN protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag2-\u003c\/span\u003e\u003cspan\u003eCDK6\u003c\/span\u003e\u003cspan\u003e protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e148×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e4\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e68μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e271μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eBSJ-03-204\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1 mM\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e200μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAnti-Tag1 Eu antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e25μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e125μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e500μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAnti-Tag2 Ac antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e25μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e125μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e500μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e15mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e75mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e300mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan\u003eNote: Aliquot into appropriate volumes, store at the recommended temperature and avoid repeated freeze-thaw cycle.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e[Process and Operations]\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e, Reagent Preparation\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e.1\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eCompositions\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eequilibration\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eBefore use, dissolve all reagents at room temperature (allow at least 30 minutes for temperature equilibration). For the 384-well plate assay, use 20 μL of reaction mixture (the reagent quantities are listed in the table below). Calculate the required volume prior to preparation accordingly. The following preparation steps are provided for reference only, using a 500-well plate as an example.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eTable 1. Reagent Preparation\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eComposition\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eConfiguration\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eμL\/well\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag1-CRBN protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan\u003eTake 20 μL of the 100× Tag1-CRBN protein stock solution, add 1.98 mL of Detection buffer to dilute it to 1× concentration, and mix thoroughly for later use.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag2-\u003c\/span\u003e\u003cspan\u003eCDK6\u003c\/span\u003e\u003cspan\u003eprotein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan\u003eTake 1\u003c\/span\u003e\u003cspan\u003e4\u003c\/span\u003e\u003cspan\u003eμL of 148×\u003c\/span\u003e\u003cspan\u003eTag2-\u003c\/span\u003e\u003cspan\u003eCDK6\u003c\/span\u003e\u003cspan\u003eprotein\u003c\/span\u003e\u003cspan\u003e, add it to\u003c\/span\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003cspan\u003e058\u003c\/span\u003e\u003cspan\u003emL of Detection buffer to dilute to 1× concentration, and mix thoroughly for later use.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAntibody\u003c\/span\u003e\u003cspan\u003eMix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTake 25 μL of the 100×\u003c\/span\u003e\u003cspan\u003eAnti-Tag1 Eu antibody\u003c\/span\u003e\u003cspan\u003estock solution, add 2475 μL of Detection buffer to dilute to 2.5 mL, and mix thoroughly; take 25 μL of the 100×\u003c\/span\u003e\u003cspan\u003eAnti-Tag2 Ac antibody\u003c\/span\u003e\u003cspan\u003estock solution, add 2475 μL of Detection buffer to dilute to 2.5 mL, and mix thoroughly; combine the two solutions in a 1:1 ratio to obtain the\u003c\/span\u003e\u003cspan\u003eAntibody\u003c\/span\u003e\u003cspan\u003eMix.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003e1.2\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eGradient dilution of the sample\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eBSJ-03-204 as an example, the dilution solution is Detection buffer. To minimize interference from matrix effects, it is recommended to use a solution with the same matrix composition as the sample being tested; additionally, the sample concentration should be adjusted according to its actual situation.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eTable\u003c\/span\u003e\u003cspan\u003e2.\u003c\/span\u003e\u003cspan\u003eBSJ-03-204 gradient dilution (Adjust according to the actual situation)\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eBSJ-03-204\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003efinal concentration (nM)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eBSJ-03-204\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003epreparation concentration (nM)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eDilution\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e①\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2000.00\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20000.00\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003cspan\u003e1 m\u003c\/span\u003e\u003cspan\u003eM\u003c\/span\u003e\u003cspan\u003eBSJ-03-204\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e98\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e②\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e800.00\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e8000.00\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003e0μL\u003c\/span\u003e\u003cspan\u003e①\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e45\u003c\/span\u003e\u003cspan\u003eμL Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e③\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e320.00\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3200.00\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003e0μL\u003c\/span\u003e\u003cspan\u003e②\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e45\u003c\/span\u003e\u003cspan\u003eμL Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e④\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e128.00\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1280.00\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003e0μL\u003c\/span\u003e\u003cspan\u003e③\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e45\u003c\/span\u003e\u003cspan\u003eμL Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑤\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e51.20\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e512.00\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003e0μL\u003c\/span\u003e\u003cspan\u003e④\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e45\u003c\/span\u003e\u003cspan\u003eμL Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑥\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e30.12\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e301.18\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003e0μL\u003c\/span\u003e\u003cspan\u003e⑤\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e21\u003c\/span\u003e\u003cspan\u003eμL Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑦\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20.08\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e200.78\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003e0μL\u003c\/span\u003e\u003cspan\u003e⑥\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e15\u003c\/span\u003e\u003cspan\u003eμL Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑧\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e8.03\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e80.31\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003e0μL\u003c\/span\u003e\u003cspan\u003e⑦\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e45\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑨\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3.21\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e32.13\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003e0μL\u003c\/span\u003e\u003cspan\u003e⑧\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e45\u003c\/span\u003e\u003cspan\u003eμL Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑩\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1.29\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e12.85\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003e0μL\u003c\/span\u003e\u003cspan\u003e⑨\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e45\u003c\/span\u003e\u003cspan\u003eμL Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑪\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e0.51\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5.14\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003e0 μL\u003c\/span\u003e\u003cspan\u003e⑩\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e45\u003c\/span\u003e\u003cspan\u003eμL Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eBlank\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e30\u003c\/span\u003e\u003cspan\u003eμL Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003e2\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e, Sample and control\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003e2.1\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eSam\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eple:\u003c\/span\u003e\u003cspan\u003eAdd 4 μL of Tag1-CRBN protein working solution, 4 μL of\u003c\/span\u003e\u003cspan\u003eTag2-\u003c\/span\u003e\u003cspan\u003eCDK6\u003c\/span\u003e\u003cspan\u003eprotein\u003c\/span\u003e\u003cspan\u003eworking solution, 2 μL of the gradient-diluted sample , and 10 μL of the mixed Antibody Mix sequentially into the 384-well plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003e2.2\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eBSJ-03-204\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e:\u003c\/span\u003e\u003cspan\u003e4 μL of Tag1-CRBN protein working solution, 4 μL of\u003c\/span\u003e\u003cspan\u003eTag2-\u003c\/span\u003e\u003cspan\u003eCDK6\u003c\/span\u003e\u003cspan\u003eprotein\u003c\/span\u003e\u003cspan\u003eworking solution, 2 μL of gradient-diluted BSJ-03-204, and 10 μL of the mixed Antibody Mix sequentially into the 384-well plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003e2.3 Blank:\u003c\/span\u003e\u003cspan\u003e4 μL of Tag1-CRBN protein working solution, 4 μL of\u003c\/span\u003e\u003cspan\u003eTag2-\u003c\/span\u003e\u003cspan\u003eCDK6\u003c\/span\u003e\u003cspan\u003eprotein\u003c\/span\u003e\u003cspan\u003eworking solution, 2 μL of\u003c\/span\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003cspan\u003e, and 10 μL of the mixed Antibody Mix sequentially into the 384-well plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003e2.3\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eNegative\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e \u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003econtrol\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e:\u003c\/span\u003e\u003cspan\u003e10μL Detection buffer\u003c\/span\u003e\u003cspan\u003e,\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eand 10 μL of the mixed Antibody Mix sequentially into the 384-well plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAfter adding all samples, centrifuge, seal with a cover membrane, and incubate at room temperature for 2 hours.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eTable 3.\u003c\/span\u003e\u003cspan\u003eSample and control spotting process\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eBSJ-03-204\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eSample\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eBlank\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNegative\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e \u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003econtrol(\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eNC)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003egradient-diluted BSJ-03-204\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003egradient-diluted sample\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"4\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e0\u003c\/span\u003e\u003cspan\u003eμL Detection buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eand 10μL Antibody Mix\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4μL Tag1-CRBN protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4 μL Tag2-\u003c\/span\u003e\u003cspan\u003eCDK6\u003c\/span\u003e\u003cspan\u003eprotein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10 μL Antibody Mix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"4\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eSeal the plate wells with a sealing film and incubate at room temperature for more than 2 hours.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e3,\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eRead Data\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe detection was performed on a TR-FRET microplate reader. The excitation wavelength was 320\/340 nm, and the emission wavelengths at 620 nm and 665 nm were detected.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e[DATA REDUCTION ]\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1)\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eRatio\u003c\/span\u003e\u003cspan\u003e:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eRatio\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e=\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e(665\/620)\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003cspan\u003e10000\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/d8426299-99bf-48cd-8b25-d8ae1e72d834.png\" style=\"margin-left:30px; width:253px; height:auto; vertical-align:middle;\"\u003e\u003c\/p\u003e\n\u003cspan\u003e2)\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eNet signal\u003c\/span\u003e\u003cspan\u003e:\u003c\/span\u003e\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eNet signal\u003c\/span\u003e\u003cspan\u003e= (Std-\u003c\/span\u003e\u003cspan\u003eNC\u003c\/span\u003e\u003cspan\u003e)\/\u003c\/span\u003e\u003cspan\u003eNC\u003c\/span\u003e\u003cspan\u003e×100\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eStd: the ratio of sample and control\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eNC:   the ratio of\u003c\/span\u003e\u003cspan\u003enegative\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003econtrol\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3)\u003c\/span\u003e\u003cspan\u003eCV(%)\u003c\/span\u003e\u003cspan\u003e:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eCV(%)= Standard Deviation\/Mean Ratio × 100%\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e[\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003eData Example\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e]\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe following data cannot replace experimental results obtained in experiments;\u003c\/span\u003e\u003cspan\u003ethey\u003c\/span\u003e\u003cspan\u003eare provided solely for illustrative purposes, and outcomes may vary depending on the plate reader used.\u003c\/span\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003eNote: Recommended microplate (384-well plate, white, shallow wells).\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"100T","offer_id":42989602701387,"sku":"UA086137-100T","price":730.0,"currency_code":"USD","in_stock":true},{"title":"500T","offer_id":42989602734155,"sku":"UA086137-500T","price":2180.0,"currency_code":"USD","in_stock":true},{"title":"2500T","offer_id":42989602766923,"sku":"UA086137-2500T","price":8175.0,"currency_code":"USD","in_stock":true},{"title":"10000T","offer_id":42989602799691,"sku":"UA086137-10000T","price":21800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_a8ea3911-6d01-4f83-8218-880441fbbc52.png?v=1793509213"},{"product_id":"unione-tr-fret-human-brd4ar-riptac-binding-kit-ua086148","title":"UniOne® TR-FRET Human BRD4\/AR RIPTAC Binding Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e【\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003eASSAY PRINCIPLE\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e】\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eThe kit \u003c\/span\u003e\u003cspan\u003eis based on \u003c\/span\u003e\u003cspan\u003ehomogeneous time-resolved fluorescence technology (TR-FRET)\u003c\/span\u003e\u003cspan\u003e technology，which is used\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eto assess \u003c\/span\u003e\u003cspan\u003ethe molecular-mediated interaction between the \u003c\/span\u003e\u003cspan\u003eBRD4 (BD1\/BD2)\u003c\/span\u003e\u003cspan\u003e and \u003c\/span\u003e\u003cspan\u003ethe LBD of human \u003c\/span\u003e\u003cspan style=\"font-weight:bold;font-style:italic;color:#000000\"\u003ea\u003c\/span\u003e\u003cspan style=\"font-weight:bold;font-style:italic;color:#000000\"\u003endrogen \u003c\/span\u003e\u003cspan style=\"font-weight:bold;font-style:italic;color:#000000\"\u003er\u003c\/span\u003e\u003cspan style=\"font-weight:bold;font-style:italic;color:#000000\"\u003eeceptor\u003c\/span\u003e\u003cspan style=\"font-weight:bold;font-style:italic;color:#000000\"\u003e (AR LBD)\u003c\/span\u003e\u003cspan\u003e. This method provides a high-throughput approach \u003c\/span\u003e\u003cspan\u003eto\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003edetect the\u003c\/span\u003e\u003cspan\u003e small molecules capable of mediating the interaction between \u003c\/span\u003e\u003cspan\u003eBRD4\u003c\/span\u003e\u003cspan\u003e and \u003c\/span\u003e\u003cspan style=\"font-weight:bold;font-style:italic;color:#000000\"\u003eAR\u003c\/span\u003e\u003cspan\u003e LBD \u003c\/span\u003e\u003cspan\u003esimpl\u003c\/span\u003e\u003cspan\u003ey\u003c\/span\u003e\u003cspan\u003e and rapid\u003c\/span\u003e\u003cspan\u003ely\u003c\/span\u003e\u003cspan\u003e .\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eAs shown in the figure below, the interaction between \u003c\/span\u003e\u003cspan\u003eBRD4\u003c\/span\u003e\u003cspan\u003e and \u003c\/span\u003e\u003cspan style=\"font-weight:bold;font-style:italic;color:#000000\"\u003eAR LBD\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003ewas detected \u003c\/span\u003e\u003cspan\u003eby \u003c\/span\u003e\u003cspan\u003ean Eu-labeled\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eanti-Tag\u003c\/span\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e antibody (TR-FRET donor) and an Ac-labeled anti-Tag\u003c\/span\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e antibody (TR-FRET receptor). Since the molecular adhesive \u003c\/span\u003e\u003cspan\u003eHLD0915\u003c\/span\u003e\u003cspan\u003e mediates the interaction between \u003c\/span\u003e\u003cspan\u003eBRD4\u003c\/span\u003e\u003cspan\u003e and \u003c\/span\u003e\u003cspan style=\"font-weight:bold;font-style:italic;color:#000000\"\u003eAR LBD\u003c\/span\u003e\u003cspan\u003e, the donor is in close proximity to the receptor antibody. Excitation of the donor antibody triggers fluorescence resonance energy transfer (FRET) to the receptor antibody, resulting in specific emission of a signal at a wavelength of 665 nm from the receptor antibody. This specific signal is proportional to the degree of interaction between molecular adhesive\u003c\/span\u003e\u003cspan\u003e,\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eBRD4\u003c\/span\u003e\u003cspan\u003e and \u003c\/span\u003e\u003cspan style=\"font-weight:bold;font-style:italic;color:#000000\"\u003eAR LBD\u003c\/span\u003e\u003cspan\u003e. This homogeneous assay is simple to perform and requires no washing steps.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/2b33e649-c1bd-4b7c-ac0e-39a7d1b1b93f.png\" style=\"margin-left:30px; width:409px; height:auto; vertical-align:middle;\" alt=\"\"\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e【Composition and Storage Conditions】\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse:collapse;width:auto;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eComposition \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003econcentration\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e100T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e2500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e10000T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eStorage \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag\u003c\/span\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e-\u003c\/span\u003e\u003cspan\u003eAR LBD\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003e0×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e8\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e40\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e8\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag\u003c\/span\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e-\u003c\/span\u003e\u003cspan\u003eBRD4\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eprotein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eHLD0915\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1 mM\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAnti-Tag\u003c\/span\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e Eu antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003e0×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e25\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e125\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAnti-Tag\u003c\/span\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e Ac antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e25\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e125\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003cspan\u003em\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e0\u003c\/span\u003e\u003cspan\u003emL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003e0\u003c\/span\u003e\u003cspan\u003emL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e40\u003c\/span\u003e\u003cspan\u003e0\u003c\/span\u003e\u003cspan\u003emL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp style=\"text-align:justify\"\u003e\u003cspan style=\"color:#000000\"\u003eNote: Aliquot into appropriate volumes, store at the recommended temperature and avoid repeated freeze-thaw cycle.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"color:#1655A5;\"\u003e\u003cstrong\u003e【Process and Operations】\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e1、Reagent Preparation\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e1.1 Compositions equilibration\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify;\"\u003e\u003cspan\u003eBefore use, dissolve all reagents at room temperature (allow at least 30 minutes for temperature equilibration). For the 384-well plate assay, use 20 μL of reaction mixture (the reagent quantities are listed in the table below). Calculate the required volume prior to preparation accordingly. The following preparation steps are provided for reference only, using a 500-well plate as an example.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan\u003eTable 1. Reagent Preparation\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:1px solid #000000;width:auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eConfiguration\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eμL\/well\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eTag1-AR LBD\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:justify;\"\u003e\u003cspan\u003eTake 40 μL of the 50× Tag1-AR LBD stock solution, add 1.96 mL of Detection buffer to dilute it to 1× concentration, and mix thoroughly for later use.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eTag2-BRD4 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:justify;\"\u003e\u003cspan\u003eTake 20 μL of 100× Tag2-BRD4 protein protein, add it to 1.98 mL of Detection buffer to dilute to 1× concentration, and mix thoroughly for later use.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eAntibody Mix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eTake 25 μL of the 100× Anti-Tag1 Eu antibody stock solution, add 2475 μL of Detection buffer to dilute to 2.5 mL, and mix thoroughly; take 25 μL of the 100× Anti-Tag2 Ac antibody stock solution, add 2475 μL of Detection buffer to dilute to 2.5 mL, and mix thoroughly; combine the two solutions in a 1:1 ratio to obtain the Antibody Mix.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e1.2 Gradient dilution of the sample\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify;\"\u003e\u003cspan\u003eHLD0915 as an example, the dilution solution is Detection buffer. To minimize interference from matrix effects, it is recommended to use a solution with the same matrix composition as the sample being tested; additionally, the sample concentration should be adjusted according to its actual situation.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan\u003eTable 2. HLD0915\u003c\/span\u003e\u003cstrong\u003e \u003c\/strong\u003e\u003cspan\u003egradient dilution（Adjust according to the actual situation）\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:1px solid #000000;width:auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eHLD0915 final concentration（nM）\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eHLD0915 preparation concentration（nM）\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eDilution\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e①\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e8000.00\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e80000.00\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e2.5μL 1 mM HLD0915 +28.75μL Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e②\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e2666.67\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e26666.67\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL ① +20μL Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e③\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e888.89\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e8888.89\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL ② +20μL Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e④\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e296.30\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e2962.96\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL ③ +20μL Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑤\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e98.77\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e987.65\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL ④ +20μL Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑥\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e32.92\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e329.22\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL ⑤ +20μL Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑦\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e10.97\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e109.74\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL ⑥ +20μL Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑧\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e3.66\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e36.58\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL ⑦+20μL Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑨\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e1.22\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e12.19\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL ⑧+20μL Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑩\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e0.41\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e4.06\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL ⑨+20μL Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eBlank\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e0\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e0\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e20μL Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2、Sample and control\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify;\"\u003e\u003cstrong\u003e2.1 Sample：\u003c\/strong\u003e\u003cspan\u003eAdd 4 μL of Tag1-AR LBD working solution, 4 μL of Tag2-BRD4 protein working solution, 2 μL of the gradient-diluted sample , and 10 μL of the mixed Antibody Mix sequentially into the 384-well plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify;\"\u003e\u003cstrong\u003e2.2 HLD0915: \u003c\/strong\u003e\u003cspan\u003e4 μL of Tag1-AR LBD working solution, 4 μL of Tag2-BRD4 protein working solution, 2 μL of gradient-diluted HLD0915, and 10 μL of the mixed Antibody Mix sequentially into the 384-well plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify;\"\u003e\u003cstrong\u003e2.3 Blank：\u003c\/strong\u003e\u003cspan\u003e4 μL of Tag1-AR LBD working solution, 4 μL of Tag2-BRD4 protein working solution,, 2 μL of Detection buffer, and 10 μL of the mixed Antibody Mix sequentially into the 384-well plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify;\"\u003e\u003cstrong\u003e2.3 Negative control：\u003c\/strong\u003e\u003cspan\u003e10μL Detection buffer, and 10 μL of the mixed Antibody Mix sequentially into the 384-well plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify;\"\u003e\u003cspan\u003eAfter adding all samples, centrifuge, seal with a cover membrane, and incubate at room temperature more than 6hours.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cstrong\u003eTable 3. Sample and control spotting process\u003c\/strong\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:1px solid #000000;width:auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eHLD0915\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eSample\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eBlank\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eNegative control(NC)\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003egradient-diluted \u003c\/span\u003e\u003cstrong\u003eHLD0915\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003egradient-diluted sample\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" rowspan=\"4\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL Detection buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eand 10μL Antibody Mix\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"3\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e4μL Tag1-AR LBD\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"3\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e4μL Tag2-BRD4 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"3\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL Antibody Mix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"4\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eSeal the plate wells with a sealing film and incubate at room temperature more than 6 hours.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"margin-right:8px;\"\u003e3、\u003c\/span\u003e\u003cstrong\u003eRead Data\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe detection was performed on a TR-FRET microplate reader. The excitation wavelength was 320\/340 nm, and the emission wavelengths at 620 nm and 665 nm were detected.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#1655A5;\"\u003e\u003cstrong\u003e【DATA REDUCTION 】\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1) Ratio：\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan\u003eRatio = (665\/620) ×10000\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2) Net signal：\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan\u003eNet signal = (Std-NC)\/NC×100\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan\u003eStd：the ratio of sample and control\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan\u003eNC: the ratio of\u003c\/span\u003e\u003cstrong\u003e negative control\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3) CV（%）：\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify;\"\u003e\u003cspan\u003eCV（%）= Standard Deviation\/Mean Ratio × 100%\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#1655A5;\"\u003e\u003cstrong\u003e【Data Example】\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe following data cannot replace experimental results obtained in experiments; they are provided solely for illustrative purposes, and outcomes may vary depending on the plate reader used.\u003c\/span\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"image_resized img img-fluid o_we_custom_image\" style=\"aspect-ratio:973\/695;width:57.2%;\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/product.template\/21151504\/d16280d6-6224-4429-b02d-2392ce5482f0.png?access_token=011a63e5-20b1-4ebc-b886-6b5f85512d84\" alt=\"\" width=\"973\" height=\"695\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cfigure class=\"image image_resized image-style-block-align-left\" style=\"width:34.46%;\"\u003e\u003cimg class=\"image_resized\" style=\"aspect-ratio:630\/531;vertical-align:middle;\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/a9531cf1-4373-487e-8ba2-65ecd13aecb7.jpeg\" alt=\"\" width=\"423\" height=\"531\"\u003e\u003c\/figure\u003e\u003cp\u003e\u003cstrong\u003eNote: Recommended microplate (384-well plate, white, shallow wells).\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"100T","offer_id":42991589032011,"sku":"UA086148-100T","price":730.0,"currency_code":"USD","in_stock":true},{"title":"500T","offer_id":42991589064779,"sku":"UA086148-500T","price":2180.0,"currency_code":"USD","in_stock":true},{"title":"2500T","offer_id":42991589097547,"sku":"UA086148-2500T","price":8175.0,"currency_code":"USD","in_stock":true},{"title":"10000T","offer_id":42991589130315,"sku":"UA086148-10000T","price":21800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_400d1870-3d13-4b43-9b68-69a4ad6c4f13.png?v=1793545227"},{"product_id":"unione-tr-fret-human-krasg12dvhl-protac-binding-kit-ua086161","title":"UniOne® TR-FRET Human KRAS[G12D]\/VHL PROTAC Binding Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eThe kit is based on homogeneous time-resolved fluorescence technology (TR-FRET) technology，which is used to assess the molecular-mediated interaction between the \u003c\/span\u003e\u003cspan\u003eKRAS[G12\u003c\/span\u003e\u003cspan\u003eD\u003c\/span\u003e\u003cspan\u003e]\u003c\/span\u003e\u003cspan\u003e and VHL\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003cspan\u003eThis method provides a high-throughput approach to detect the small molecules capable of mediating the interaction between the KRAS[G12D] and VHL\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003esimply and rapidly.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eAs shown in the figure below, the interaction between KRAS[G12D] and VHL\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003ewas detected by an Eu-labeled anti-Tag1 antibody (TR-FRET donor) and an Ac-labeled anti-Tag2 antibody (TR-FRET receptor). Since the \u003c\/span\u003e\u003cspan\u003ePROTAC\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003emolecule \u003c\/span\u003e\u003cspan\u003eACBI3\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003emediates the interaction between KRAS[G12D] and VHL, the donor antibody is in close proximity to the receptor antibody. Excitation of the donor antibody triggers fluorescence resonance energy transfer (FRET) to the receptor antibody, resulting in specific emission of a signal at a wavelength of 665 nm from the receptor antibody. This specific signal is proportional to the degree of interaction between ACBI3\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e, KRAS[G12D] and \u003c\/span\u003e\u003cspan\u003eVHL\u003c\/span\u003e\u003cspan\u003e. This homogeneous assay is simple to perform and requires no washing steps.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/495d681e-d0b3-4ed2-91b0-024449160ad9.png\" style=\"margin-left:30px; width:482px; height:auto; vertical-align:middle;\" alt=\"\"\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"color:#1655A5;\"\u003e\u003cstrong\u003e【Composition and Storage Conditions】\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:1px solid #000000;width:auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003econcentration\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003e100T\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003e500T\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003e2500T\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003e10000T\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eTag1-KRAS[G12D] protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e5μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e20μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e100μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eTag2-VHL protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e50×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e8μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e40μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e200μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e800μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eACBI3\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e1 mM\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e5μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e200μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eAnti-Tag1 Eu antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e50×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e250μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e1000μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eAnti-Tag2 Ac antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e12.5×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e40μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e200μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e1mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e4mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e2mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e40mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eNote: Aliquot into appropriate volumes, store at the recommended temperature and avoid repeated freeze-thaw cycle.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e \u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"color:#1655A5;\"\u003e\u003cstrong\u003e【Process and Operations】\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e1、Reagent Preparation\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e1.1 Compositions equilibration\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify;\"\u003e\u003cspan\u003eBefore use, dissolve all reagents at room temperature (allow at least 30 minutes for temperature equilibration). For the 384-well plate assay, use 20 μL of reaction mixture (the reagent quantities are listed in the table below). Calculate the required volume prior to preparation accordingly. The following preparation steps are provided for reference only, using a 500-well plate as an example.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan\u003eTable 1. Reagent Preparation\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:1px solid #000000;width:auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eConfiguration\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eμL\/well\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:justify;\"\u003e\u003cspan\u003eAdd 2 mL of 10× Detection buffer to 18 mL of deionized water, dilute to 1× Detection , and mix thoroughly for later use.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e-\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eTag1-KRAS[G12D] protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:justify;\"\u003e\u003cspan\u003eTake 20 μL of the 100× Tag1-KRAS[G12D] protein stock solution, add 1.98 mL of 1× Detection buffer to dilute it to 1× concentration, and mix thoroughly for later use.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eTag2-VHL protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:justify;\"\u003e\u003cspan\u003eTake 40 μL of 50× Tag2-VHL protein, add it to 1.96 mL of 1× Detection buffer to dilute to 1× concentration, and mix thoroughly for later use.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eAntibody Mix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eTake 50 μL of the 50× Eu-anti-Tag1 stock solution, add 2450 μL of 1× Detection buffer to dilute to 2.5 mL, and mix thoroughly; take 200 μL of the 12.5× Ac-anti-Tag2 stock solution, add 2300 μL of 1× Detection buffer to dilute to 2.5 mL, and mix thoroughly; combine the two solutions in a 1:1 ratio to obtain the Antibody Mix.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e1.2 Gradient dilution of the sample\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eACBI3 as an example, the dilution solution is 1× Detection buffer. To minimize interference from matrix effects, it is recommended to use a solution with the same matrix composition as the sample being tested; additionally, the sample concentration should be adjusted according to its actual situation.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan\u003eTable 2. ACBI3 gradient dilution（Adjust according to the actual situation）\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:1px solid #000000;width:auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eACBI3 final concentration（nM）\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eACBI3 preparation concentration（nM）\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eDilution\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e①\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e9000\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e90000\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e2uL 1mM Daraxonrasib +20uL1x Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e②\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e3000.00\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e30000.00\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL ① +20μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e③\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e1000.00\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e10000.00\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL ② +20μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e④\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e333.33\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e3333.33\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL ③ +20μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑤\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e111.11\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e1111.11\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL ④ +20μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑥\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e37.04\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e370.37\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL ⑤ +20μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑦\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e12.35\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e123.46\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL ⑥ +20μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑧\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e4.12\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e41.15\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL ⑦+20μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑨\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e1.37\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e13.72\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL ⑧+20μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑩\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e0.46\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e4.57\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL ⑨+20μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑪\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e0.15\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e1.52\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL ⑩+20μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eBlank\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e0\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e0\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e20μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2、Sample and control\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2.1 Sample：\u003c\/strong\u003e\u003cspan\u003eAdd 4 μL of Tag1-KRAS[G12D] protein working solution, 4 μL of Tag2-VHL protein working solution, 2 μL of the gradient-diluted sample , and 10 μL of the mixed Antibody Mix sequentially into the 384-well plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2.2 ACBI3 :\u003c\/strong\u003e\u003cspan\u003e 4 μL of Tag1-KRAS[G12D] protein working solution, 4 μL of Tag2-VHL protein working solution, 2 μL of gradient-diluted Daraxonrasib, and 10 μL of the mixed Antibody Mix sequentially into the 384-well plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2.3 Blank：\u003c\/strong\u003e\u003cspan\u003e4 μL of Tag1-KRAS[G12D] protein working solution, 4 μL of Tag2-VHL protein working solution, 2 μL of Detection buffer, and 10 μL of the mixed Antibody Mix sequentially into the 384-well plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2.4 Negative control：\u003c\/strong\u003e\u003cspan\u003e 10μL Detection buffer, and 10 μL of the mixed Antibody Mix sequentially into the 384-well plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAfter adding all samples, centrifuge, seal with a cover membrane, and incubate at room temperature for 1 hours.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan\u003eTable 3. Sample and control spotting process\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:1px solid #000000;width:auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eDaraxonrasib\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eSample\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eBlank\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eNegative control (NC)\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003egradient-diluted Daraxonrasib\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003egradient-diluted sample\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" rowspan=\"4\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eand 10μL Antibody Mix\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"3\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e4μL Tag1-KRAS[G12D] protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"3\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e4μL Tag2-VHL protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"3\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL Antibody Mix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"4\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eSeal the plate wells with a sealing film and incubate at room temperature for 1hours.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"margin-right:8px;\"\u003e3、\u003c\/span\u003e\u003cstrong\u003eRead Data\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe detection was performed on a TR-FRET microplate reader. The excitation wavelength was 320\/340 nm, and the emission wavelengths at 620 nm and 665 nm were detected.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#1655A5;\"\u003e\u003cstrong\u003e【DATA REDUCTION 】\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1) Ratio：\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan\u003eRatio = (665\/620) ×10000\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2) Net signal：\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan\u003eNet signal = (Std-NC)\/NC×100\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eStd：the ratio of sample and control\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eNC: the ratio of negative control\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3) CV（%）：\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan\u003eCV（%）= Standard Deviation\/Mean Ratio × 100%\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#1655A5;\"\u003e\u003cstrong\u003e【Data Example】\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe following data cannot replace experimental results obtained in experiments; they are provided solely for illustrative purposes, and outcomes may vary depending on the plate reader used.\u003c\/span\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"image_resized\" style=\"height:auto;margin-left:30px;vertical-align:middle;width:437px;\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/ff80113c-89ed-42db-b8cc-2c922a909950.jpeg\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003cstrong\u003eNote: Recommended microplate\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e(384-well plate, white, shallow wells).\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"100T","offer_id":43045168808011,"sku":"UA086161-100T","price":730.0,"currency_code":"USD","in_stock":true},{"title":"500T","offer_id":43045168840779,"sku":"UA086161-500T","price":2180.0,"currency_code":"USD","in_stock":true},{"title":"2500T","offer_id":43045168873547,"sku":"UA086161-2500T","price":8175.0,"currency_code":"USD","in_stock":true},{"title":"10000T","offer_id":43045168906315,"sku":"UA086161-10000T","price":21800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_1479d343-93fd-4a8e-8b6e-b072fcde6510.png?v=1793574199"},{"product_id":"unione-tr-fret-human-nek7crbn-protac-binding-kit-ua086131","title":"UniOne® TR-FRET Human NEK7\/CRBN PROTAC Binding Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-80℃\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"text-align:justify;\"\u003e\u003cspan\u003eThe kit is based on homogeneous time-resolved fluorescence technology (TR-FRET) technology，which is used to assess the molecular-mediated interaction between the DDB1\/CRBN complex and NEK7. This method provides a high-throughput approach to detect the small molecules capable of mediating the interaction between the DDB1\/CRBN complex and NEK7 simply and rapidly .\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify;\"\u003e\u003cspan\u003eAs shown in the figure below, the interaction between DDB1\/CRBN and NEK7 was detected by an Eu-labeled anti-Tag1 antibody (TR-FRET donor) and an Ac-labeled anti-Tag2 antibody (TR-FRET receptor). Since the molecular adhesive NK7-902 diTFA mediates the interaction between DDB1\/CRBN and NEK7, the donor antibody is in close proximity to the receptor antibody. Excitation of the donor antibody triggers fluorescence resonance energy transfer (FRET) to the receptor antibody, resulting in specific emission of a signal at a wavelength of 665 nm from the receptor antibody. This specific signal is proportional to the degree of interaction between NK7-902 diTFA, DDB1\/CRBN and NEK7. This homogeneous assay is simple to perform and requires no washing steps.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"image_resized\" style=\"aspect-ratio:1255\/706;margin-left:30px;vertical-align:middle;width:75%;\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/6343dbeb-96c7-485d-95fa-d04f3edf468d.png\" alt=\"\" width=\"1255\" height=\"706\"\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003ctable border=\"1\" style=\"border-collapse:collapse;width:auto;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eComposition \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003econcentration\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e100T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e2500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e10000T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eStorage \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag1-\u003c\/span\u003e\u003cspan\u003eCRBN \u003c\/span\u003e\u003cspan\u003eprotein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003e0×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag2-\u003c\/span\u003e\u003cspan\u003eNEK7\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eprotein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e20\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eNK7-902 diTFA\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e500 μM\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAnti-Tag1 Eu antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003e0×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e25\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e125\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAnti-Tag2 Ac antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e200\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e13\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e63\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e250\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e40mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e```html\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#1655A5;\"\u003e\u003cstrong\u003e【Process and Operations】\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e1、Reagent Preparation\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e1.1 Compositions equilibration\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify;\"\u003e\u003cspan\u003eBefore use, dissolve all reagents at room temperature (allow at least 30 minutes for temperature equilibration). For the 384-well plate assay, use 20 μL of reaction mixture (the reagent quantities are listed in the table below). Calculate the required volume prior to preparation accordingly. The following preparation steps are provided for reference only, using a 500-well plate as an example.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan\u003eTable 1. Reagent Preparation\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:1px solid #000000;width:auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003ePreparation\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eμL\/well\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:justify;\"\u003e\u003cspan\u003eAdd 2 mL of 10× Detection buffer to 18 mL of deionized water, dilute to 1× Detection , and mix thoroughly for later use.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e-\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eTag1-CRBN protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:justify;\"\u003e\u003cspan\u003eTake 20 μL of the 100× Tag1-CRBN protein stock solution, add 1.98 mL of 1× Detection buffer to dilute it to 1× concentration, and mix thoroughly for later use.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eTag2-NEK7 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:justify;\"\u003e\u003cspan\u003eTake 20 μL of 100× Tag2-NEK7 protein, add it to 1.98 mL of 1× Detection buffer to dilute to 1× concentration, and mix thoroughly for later use.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eAntibody Mix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eTake 25 μL of the 100× Eu-anti-Tag1 stock solution, add 2475 μL of 1× Detection buffer to dilute to 2.5 mL, and mix thoroughly; take 13 μL of the 200× Ac-anti-Tag2 stock solution, add 2587 μL of 1× Detection buffer to dilute to 2.6 mL, and mix thoroughly; combine the two solutions in a 1:1 ratio to obtain the Antibody Mix.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e1.2 Gradient dilution of the sample\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eNK7-902 diTFA as an example, the dilution solution is 1× Detection buffer. To minimize interference from matrix effects, it is recommended to use a solution with the same matrix composition as the sample being tested; additionally, the sample concentration should be adjusted according to its actual situation.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan\u003eTable 2. \u003c\/span\u003e\u003cstrong\u003eNK7-902 diTFA \u003c\/strong\u003e\u003cspan\u003egradient dilution（Adjust according to the actual situation）\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:1px solid #000000;width:auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eNK7-902 diTFA final concentration（nM）\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eNK7-902 diTFA preparation concentration（nM）\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eDilution\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e①\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e500.00\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e5000.00\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e1μL 500 μM NK7-902 diTFA +99μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e②\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e250.00\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e2500.00\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e40μL ① +40μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e③\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e125.00\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e1250.00\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e40μL ② +40μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e④\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e62.50\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e625.00\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e40μL ③ +40μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑤\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e31.25\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e312.50\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e40μL ④ +40μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑥\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e15.63\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e156.25\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e40μL ⑤ +40μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑦\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e7.81\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e78.13\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e40μL ⑥ +40μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑧\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e3.91\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e39.06\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e40μL ⑦+40μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑨\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e1.95\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e19.53\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e40μL ⑧+40μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑩\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e0.98\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e9.77\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e40μL ⑨+40μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e⑪\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e0.49\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:#000000;\"\u003e\u003ci\u003e\u003cu\u003e4.88\u003c\/u\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e40μL ⑩+40μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eBlank\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e40μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2、Sample and control\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2.1 Sample：\u003c\/strong\u003e\u003cspan\u003eAdd 4 μL of Tag1-CRBN protein working solution, 4 μL of Tag2-NEK7 protein working solution, 2 μL of the gradient-diluted sample , and 10 μL of the mixed Antibody Mix sequentially into the 384-well plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2.2 NK7-902 diTFA:\u003c\/strong\u003e\u003cspan\u003e 4 μL of Tag1-CRBN protein working solution, 4 μL of Tag2-NEK7 protein working solution, 2 μL of gradient-diluted NK7-902 diTFA, and 10 μL of the mixed Antibody Mix sequentially into the 384-well plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2.3 Blank：\u003c\/strong\u003e\u003cspan\u003e4 μL of Tag1-CRBN protein working solution, 4 μL of Tag2-NEK7 protein working solution, 2 μL of Detection buffer, and 10 μL of the mixed Antibody Mix sequentially into the 384-well plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2.3 Negative control：\u003c\/strong\u003e\u003cspan\u003e 10μL Detection buffer, and 10 μL of the mixed Antibody Mix sequentially into the 384-well plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAfter adding all samples, centrifuge, seal with a cover membrane, and incubate at room temperature for 2 hours.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cstrong\u003eTable 3. Sample and control spotting process\u003c\/strong\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:1px solid #000000;width:auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eNK7-902 diTFA\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eSample\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eBlank\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eNegative control(NC)\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003egradient-diluted NK7-902 diTFA\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003egradient-diluted sample\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" rowspan=\"4\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eand 10μL Antibody Mix\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"3\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e4μL Tag1-CRBN protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"3\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e4μL Tag2-NEK7 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"3\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e10μL Antibody Mix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"4\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eSeal the plate wells with a sealing film and incubate at room temperature for 2 hours.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"margin-right:8px;\"\u003e3、\u003c\/span\u003e\u003cstrong\u003eRead Data\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe detection was performed on a TR-FRET microplate reader. The excitation wavelength was 320\/340 nm, and the emission wavelengths at 620 nm and 665 nm were detected.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#1655A5;\"\u003e\u003cstrong\u003e【DATA REDUCTION 】\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1) Ratio：\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan\u003eRatio = (665\/620) ×10000\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2) Net signal：\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan\u003eNet signal = (Std-NC)\/NC×100\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan\u003eStd：the ratio of sample and control\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan\u003eNC: the ratio of\u003c\/span\u003e\u003cstrong\u003e negative control\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3) CV（%）：\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan\u003eCV（%）= Standard Deviation\/Mean Ratio × 100%\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#1655A5;\"\u003e\u003cstrong\u003e【Data Example】\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe following data cannot replace experimental results obtained in experiments; they are provided solely for illustrative purposes, and outcomes may vary depending on the plate reader used.\u003c\/span\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"image_resized\" style=\"aspect-ratio:552\/357;margin-left:30px;vertical-align:middle;width:75%;\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/0e13bad4-ff11-46e8-912e-515da60edb88.png\" alt=\"\" width=\"552\" height=\"357\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote: Recommended microplate (384-well plate, white, shallow wells).\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e```\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"100T","offer_id":43093643722827,"sku":"UA086131-100T","price":730.0,"currency_code":"USD","in_stock":true},{"title":"500T","offer_id":43093643755595,"sku":"UA086131-500T","price":2180.0,"currency_code":"USD","in_stock":true},{"title":"2500T","offer_id":43093643788363,"sku":"UA086131-2500T","price":8175.0,"currency_code":"USD","in_stock":true},{"title":"10000T","offer_id":43093643821131,"sku":"UA086131-10000T","price":21800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_d684af1a-ce40-4bf9-bae4-e8d4ac843ac2.png?v=1793498576"},{"product_id":"unione-tr-fret-human-ck1-crbn-protac-binding-kit-ua086134","title":"UniOne® TR-FRET Human CK1 α\/CRBN PROTAC Binding Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-80℃\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003eThe kit is based on homogeneous time-resolved fluorescence technology (TR-FRET) technology，which is used to assess the molecular-mediated interaction between the DDB1\/CRBN complex and CK1 α. This method provides a high-throughput approach to detect the small molecules capable of mediating the interaction between the DDB1\/CRBN complex and CK1 α simply and rapidly .\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAs shown in the figure below, the interaction between DDB1\/CRBN and CK1 α was detected by an Eu-labeled anti-Tag1 antibody (TR-FRET donor) and an Ac-labeled anti-Tag2 antibody (TR-FRET receptor). Since the molecular adhesive DEG-35 mediates the interaction between DDB1\/CRBN and CK1 α, the donor antibody is in close proximity to the receptor antibody. Excitation of the donor antibody triggers fluorescence resonance energy transfer (FRET) to the receptor antibody, resulting in specific emission of a signal at a wavelength of 665 nm from the receptor antibody. This specific signal is proportional to the degree of interaction between DEG-35, DDB1\/CRBN and CK1 α. This homogeneous assay is simple to perform and requires no washing steps.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/37043b1f-3ed8-4480-94f1-46fca47751a9.png\" style=\"margin-left:30px; width:533px; height:auto; vertical-align:middle;\" alt=\"\"\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003ctable border=\"1\" style=\"border-collapse:collapse;width:auto;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eComposition \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003econcentration\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e100T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e2500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e10000T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eStorage \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag1-CRBN protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e200×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e200μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag2-\u003c\/span\u003e\u003cspan\u003eCK1 α\u003c\/span\u003e\u003cspan\u003e protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e40×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e0\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e250μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDEG-35\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e300 μM\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e50μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e200μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAnti-Tag1 Eu antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e25μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e125μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e500μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAnti-Tag2 Ac antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e200×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e13μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e63μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e250μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e400μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e40mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"color:#000000\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eNote: Aliquot into appropriate volumes, store at the recommended temperature and avoid repeated freeze-thaw cycle.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003eHere is the translated content in English, maintaining the original formatting:```html\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e、Reagent Preparation\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e.1 \u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eCompositions \u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eequilibration\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eBefore use, dissolve all reagents at room temperature (allow at least 30 minutes for temperature equilibration). For the 384-well plate assay, use 20 μL of reaction mixture (the reagent quantities are listed in the table below). Calculate the required volume prior to preparation accordingly. The following preparation steps are provided for reference only, using a 500-well plate as an example.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"color:#000000\"\u003eTable 1. Reagent Preparation\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eComposition \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003ePreparation\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eμL\/well\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDetection\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003ebuffer\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan\u003eAdd 2 mL of 10× Detection buffer to 18 mL of deionized water, dilute to 1× Detection , and mix thoroughly for later use.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag1-CRBN protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan\u003eTake 10 μL of the 200× Tag1-CRBN protein stock solution, add 1.99 mL of 1× Detection buffer to dilute it to 1× concentration, and mix thoroughly for later use.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag2-\u003c\/span\u003e\u003cspan\u003eCK1 α\u003c\/span\u003e\u003cspan\u003e protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp\u003e\u003cspan\u003eTake 50 μL of 40× Tag2-\u003c\/span\u003e\u003cspan\u003eCK1 α\u003c\/span\u003e\u003cspan\u003e protein, add it to 1.95 mL of 1× Detection buffer to dilute to 1× concentration, and mix thoroughly for later use.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAntibody \u003c\/span\u003e\u003cspan\u003eMix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTake 25 μL of the 100× Eu-anti-Tag1 stock solution, add 2475 μL of 1× Detection buffer to dilute to 2.5 mL, and mix thoroughly; take 13 μL of the 200× Ac-anti-Tag2 stock solution, add 2587 μL of 1× Detection buffer to dilute to 2.6 mL, and mix thoroughly; combine the two solutions in a 1:1 ratio to obtain the \u003c\/span\u003e\u003cspan\u003eAntibody \u003c\/span\u003e\u003cspan\u003eMix.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan style=\"color:#000000\"\u003e1.2 Gradient dilution of the sample \u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"color:#000000\"\u003eDEG-35 as an example, the dilution solution is 1× Detection buffer. To minimize interference from matrix effects, it is recommended to use a solution with the same matrix composition as the sample being tested; additionally, the sample concentration should be adjusted according to its actual situation.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"color:#000000\"\u003eTable 2. DEG-35 gradient dilution（Adjust according to the actual situation）\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eDEG-35 \u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003efinal concentration（nM）\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eDEG-35 \u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003epreparation concentration（nM）\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eDilution\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e①\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e300\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003eμL \u003c\/span\u003e\u003cspan\u003e300 μ\u003c\/span\u003e\u003cspan\u003eM \u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eDEG-35\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e99\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e②\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100 \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1000 \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003e0μL \u003c\/span\u003e\u003cspan\u003e①\u003c\/span\u003e\u003cspan\u003e +\u003c\/span\u003e\u003cspan\u003e60\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e③\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e33.33 \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e333.33 \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003e0μL \u003c\/span\u003e\u003cspan\u003e②\u003c\/span\u003e\u003cspan\u003e +\u003c\/span\u003e\u003cspan\u003e60\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e④\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e11.11 \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e111.11 \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003e0μL \u003c\/span\u003e\u003cspan\u003e③\u003c\/span\u003e\u003cspan\u003e +\u003c\/span\u003e\u003cspan\u003e60\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑤\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3.70 \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e37.04 \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003e0μL \u003c\/span\u003e\u003cspan\u003e④\u003c\/span\u003e\u003cspan\u003e +\u003c\/span\u003e\u003cspan\u003e60\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑥\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1.23 \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e12.35 \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003e0μL \u003c\/span\u003e\u003cspan\u003e⑤\u003c\/span\u003e\u003cspan\u003e +\u003c\/span\u003e\u003cspan\u003e60\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑦\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e0.41 \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4.12 \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003e0μL \u003c\/span\u003e\u003cspan\u003e⑥\u003c\/span\u003e\u003cspan\u003e +\u003c\/span\u003e\u003cspan\u003e60\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑧\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e0.137 \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1.37 \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003e0μL \u003c\/span\u003e\u003cspan\u003e⑦\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e60\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑨\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e0.046 \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e0.46 \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003e0μL \u003c\/span\u003e\u003cspan\u003e⑧\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e60\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑩\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e0.015 \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e0.15 \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003e0μL \u003c\/span\u003e\u003cspan\u003e⑨\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e60\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e⑪\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e0.005 \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e0.051 \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003e0μL \u003c\/span\u003e\u003cspan\u003e⑩\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e60\u003c\/span\u003e\u003cspan\u003eμL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eBlank\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e0 \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e6\u003c\/span\u003e\u003cspan\u003e0μL 1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003e2、Sample and control\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003e2.1 Sample：\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eAdd 4 μL of Tag1-CRBN protein working solution, 4 μL of Tag2-CK1 α protein working solution, 2 μL of the gradient-diluted sample , and 10 μL of the mixed Antibody Mix sequentially into the 384-well plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003e2.2 DEG-35: \u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e4 μL of Tag1-CRBN protein working solution, 4 μL of Tag2-CK1 α protein working solution, 2 μL of gradient-diluted DEG-35, and 10 μL of the mixed Antibody Mix sequentially into the 384-well plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003e2.3 Blank：\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e4 μL of Tag1-CRBN protein working solution, 4 μL of Tag2-CK1 α protein working solution, 2 μL of Detection buffer, and 10 μL of the mixed Antibody Mix sequentially into the 384-well plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003e2.3 Negative control： \u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e10μL Detection buffer, and 10 μL of the mixed Antibody Mix sequentially into the 384-well plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003eAfter adding all samples, centrifuge, seal with a cover membrane, and incubate at room temperature for 2 \u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eh\u003c\/span\u003e\u003cspan\u003eours\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003eTable 3. Sample and control spotting process\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eDEG-35\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eSample\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eBlank\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNegative\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e \u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003econtrol(\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eNC)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003egradient-diluted \u003c\/span\u003e\u003cspan\u003eDEG-35\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003egradient-diluted sample\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1× Detection buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"4\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e0\u003c\/span\u003e\u003cspan\u003eμL 1\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003cspan\u003e Detection buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eand 10μL Antibody Mix\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4μL Tag1-CRBN protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4μL Tag2-\u003c\/span\u003e\u003cspan\u003eCK1 α\u003c\/span\u003e\u003cspan\u003e protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10μL Antibody Mix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"4\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eSeal the plate wells with a sealing film and incubate at room temperature for 2 hours.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e3、\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003eRead Data\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003eThe detection was performed on a TR-FRET microplate reader. The excitation wavelength was 320\/340 nm, and the emission wavelengths at 620 nm and 665 nm were detected.\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e【\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003eDATA REDUCTION 】\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003e1) Ratio：\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003eRatio = (665\/620) ×10000\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003e2) Net signal： \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003eNet signal = (Std-NC)\/NC×100\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003eStd：the ratio of sample and control\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003eNC: the ratio of negative control\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003e3) CV（%）：\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"color:#000000\"\u003e        CV（%）= Standard Deviation\/Mean Ratio × 100%\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e【\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003eData Example】\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003eThe following data cannot replace experimental results obtained in experiments; they are provided solely for illustrative purposes, and outcomes may vary depending on the plate reader used.\u003c\/span\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/21e77159-d1de-4a89-9cb9-540cea192400.png\" style=\"margin-left:30px; width:456px; height:auto; vertical-align:middle;\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#000000\"\u003eNote: Recommended microplate (384-well plate, white, shallow wells).\u003c\/span\u003e\u003c\/p\u003e```\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"100T","offer_id":43093864382539,"sku":"UA086134-100T","price":730.0,"currency_code":"USD","in_stock":true},{"title":"500T","offer_id":43093864415307,"sku":"UA086134-500T","price":2180.0,"currency_code":"USD","in_stock":true},{"title":"2500T","offer_id":43093864448075,"sku":"UA086134-2500T","price":8175.0,"currency_code":"USD","in_stock":true},{"title":"10000T","offer_id":43093864480843,"sku":"UA086134-10000T","price":21800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_6766ccd3-65a5-43d2-b4b4-69ea81f20b27.png?v=1793502174"}],"url":"https:\/\/www.antbioinc.com\/collections\/tpd-assay-kits.oembed?page=2","provider":"AntBio","version":"1.0","type":"link"}