{"title":"T Cell Detection \u0026 Stimulation Kit","description":null,"products":[{"product_id":"cell-titer-turbo-3d-luminescent-cell-viability-assay","title":"Cell Titer Turbo 3D Luminescent Cell Viability Assay","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eReagent Preparation:\u003c\/strong\u003e\u003cbr\u003e1. Melting of reagents: Take out the reagents the day before the experiment and place them at 4 °C overnight to melt. The reagent can also be taken out on the day of the experiment and melted at room temperature, or melted in a 22 °C water bath, but it should be noted that the water temperature should not exceed 25 °C.\u003cbr\u003e2. Equilibrate to room temperature: If the reagent melts at non-room temperature conditions, it can be placed in a 22 °C water bath before use to ensure that the reagent is balanced to room temperature before being used for testing. Note: Generally, it takes about 10 minutes for 5mL packaging; The 50 mL packaging takes about 20 minutes.\u003cbr\u003e3. Mix well: Gently invert the solution 5 times before use to mix the solution evenly.\u003cbr\u003e\u003cstrong\u003eDetection Procedure:\u003c\/strong\u003e\u003cbr\u003e1. Use a 96-well plate suitable for chemiluminescence detection, and add microtissue containing cell culture medium to it.\u003cbr\u003e\u003cstrong\u003eNote: (1) The prepared sample volume and microtissue characteristics (e.g., size, quantity, culture days, etc.) should be optimized for experimental conditions; (2) The total ATP content of the sample should be kept below 10μM (determine the upper limit concentration of the linear range); (3) The multi-well plate must be compatible with the photometer used.\u003c\/strong\u003e\u003cbr\u003e2. Add test compounds to experimental wells and incubate according to your culture protocol.\u003cbr\u003e\u003cstrong\u003eNote: Ensure that the volume of sample and test compound is low enough to allow the addition of equal volumes of reagent and then mixing without inter-well contamination.\u003c\/strong\u003e\u003cbr\u003e3. Equilibrate the plate and its contents to room temperature (22-25 °C) for about 30 minutes.\u003cbr\u003e4. Add 3D Reagent equal to the volume of cell culture medium present in each well to the detection well (e.g., for a 96-well plate, add 100 μl of 3D Reagent to 100 μl of cell-containing culture medium).\u003cbr\u003e5. The contents were vigorously mixed for 5 minutes to induce cell lysis.\u003cbr\u003e\u003cstrong\u003eNote: Mixing is important for efficient ATP extraction from 3D microtissues.\u003c\/strong\u003e\u003cbr\u003e6. Allow the plate to incubate at room temperature for another 25 minutes to stabilize the luminescence signal.\u003cbr\u003e7. Record luminescence.\u003cbr\u003e\u003cstrong\u003eNote: The non-uniform luminescence signal within the plate may be caused by temperature gradients, non-uniform cell seeding, or edge effects in multi-well plates.\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e3D cell cultures are more physiologically relevant and better representative of tissues in vivo, allowing more accurate predictions of efficacy or toxicity of drug treatments. 3D cell models are increasingly being used to understand disease mechanisms and discover drug therapies. Compared with 2D culture, 3D cell culture can predict the efficacy or toxicity of drug treatment more accurately.\u003cbr\u003e3D Luminescent Cell Viability Assay Kit is a cell viability detection reagent based on luciferase system. It uses ATP-dependent luciferase-catalyzed luciferin luminescence reaction to measure intracellular ATP content through chemiluminescence signal to detect cell viability. The detection has wide linear range, high sensitivity and good stability.\u003cbr\u003eDue to the high-purity luciferin substrate, thermostable luciferase and optimized reaction reagents in this kit, this product has stronger cell lysis ability and can be used for cell viability analysis of 3D microtissue culture. This product is added to the cell culture to lyse the cell mass and release ATP, which can produce the reaction as shown in the figure and send out a stable light signal. The luminescence intensity is directly proportional to the amount of ATP, that is, the number of viable cells, within a certain range, so this product can quantitatively detect the number of viable cells.\u003cbr\u003e\u003cimg src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20231218\/595f7cf0567f40e98da696cd181a6cfc.png\" alt=\"\" width=\"493\" height=\"159\"\u003e\u003cbr\u003ePRODUCT FEATURES\u003cbr\u003eThe 3D Luminescent Cell Viability Assay has high detection sensitivity and wide linear range, and is compatible with small sample detection and high-throughput screening of large samples.\u003cbr\u003e1. Convenient and fast: The detection reagents provided in the kit are ready-to-use, with stable readings and fast detection speed. It only takes about 10 minutes to complete the detection;\u003cbr\u003e2. High sensitivity: It has a higher signal-to-noise ratio when applied to 3D microtissue detection;\u003cbr\u003e3. Fast: data can be recorded within 30 minutes or less after adding reagents;\u003cbr\u003e4. The luminescent signal is more stable: the half-life of the luminescent signal is ultra-stable, usually \u0026gt; 3 hours.\u003cbr\u003e5. Stronger lysis ability: The optimized reaction reagent has stronger cell lysis ability and is suitable for cell viability analysis of 3D microtissue culture;\u003cbr\u003e6. High throughput: It is compatible with the detection of a small amount of samples and the high throughput screening of a large number of samples.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Temperature: The reagent contains luciferase, and repeated freezing and thawing will affect its activity. It is recommended to store at-20 ℃ in the dark from light after dispensing. Reagents and cell samples should be balanced to room temperature before use to avoid the influence of enzyme catalytic effect;\u003cbr\u003e2. Chemical factors: The reaction rate and luminous intensity of luciferase are affected by the chemical environment. There are differences in luminescence intensity and attenuation rate among different types of media and serum. In addition, high drug content may interfere with the luciferase reaction, thus affecting the luminescence signal. It is recommended to set up control wells of cell culture medium containing drugs to eliminate the interference of solvents;\u003cbr\u003e3. Light sensitivity: This reagent is sensitive to light. If it is exposed to light during storage, it will accelerate the attenuation of luminous intensity. If the reagent is transferred from the original container, make sure to keep it protected from light;\u003cbr\u003e4. ATP contamination: It is recommended to wear masks and latex gloves during operation to avoid contact with surfaces and equipment that may be contaminated. Avoid inserting the tip of the gun tip into the vial multiple times during operation.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eInstructions\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003ereagent preparation: \u003c\/strong\u003e\u003cbr\u003e1, reagent melting: The day before the experiment, the reagent was removed and placed at 4 ° C overnight to melt. It is also possible to remove the reagent on the day of the experiment and melt it at room temperature or place it in a 22℃ water bath, but it should be noted that the water temperature should not exceed 25℃. \u003cbr\u003e2, balance to room temperature: if the reagent melts under non-room temperature conditions, it can be placed in a 22 ° C water bath before use, to ensure that the reagent is balanced to room temperature before detection. Note: Generally, it takes about 10min for 5mL packaging; 50mL packaging takes about 20min. \u003cbr\u003e3, mixing: Before use, gently invert 5 times to mix the solution evenly. \u003cbr\u003e\u003cstrong\u003edetection steps: \u003c\/strong\u003e\u003cbr\u003e1, use a 96-well plate suitable for chemiluminescence detection, in which microtissue containing cell culture medium is added. \u003cbr\u003e\u003cstrong\u003eNote: (1) The preparation sample volume and microtissue characteristics (e.g., size, number, incubation days, etc.) should be optimized for the experimental conditions; (2) The total ATP content of the sample should be maintained at 10μ \u003cm of the upper limit linear range concentration multiwell plate must be compatible with photometer used.\u003e\u003cbr\u003e2, Add the test compound to the experimental well and incubate according to your culture protocol. \u003cbr\u003e\u003cstrong\u003eNote: Make sure the volume of the sample and test compound is low enough to allow the addition of an equal volume of reagent, followed by mixing, without interwell contamination. \u003c\/strong\u003e\u003cbr\u003e3, Equilibrate the plate and its contents to room temperature (22-25 ° C) for about 30 minutes. \u003cbr\u003e4, Add Cell Titer Turbo 3D Reagent equal to the volume of cell medium present in each well to the assay Wells (for example, for a 96-well plate, add 100μ l Cell Titer Turbo 3D Reagent was added to 100μ l in the medium containing the cells). \u003cbr\u003e5, mix the contents vigorously for 5 min to induce cell lysis. \u003cbr\u003e\u003cstrong\u003eNote: Mixing is important for efficient ATP extraction from 3D microtissues. \u003c\/strong\u003e\u003cbr\u003e6, Allow the plate to incubate at room temperature for an additional 25 minutes to stabilize the luminescence signal.  strong \u0026gt;\u003cbr\u003e7, record luminescence. \u003cbr\u003e\u003cstrong\u003eNote: The non-uniform luminescence signal in the plate may be caused by temperature gradients, uneven cell seeding, or edge effects in the multi-well plate. \u003c\/strong\u003e\u003c\/m\u003e\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Store below-10 °C and protect from light. In order to ensure the best performance, it is recommended to place it at-70 ℃ for long-term storage.\u003cbr\u003e2. This product can still remain stable after 6 cycles of repeated freezing and thawing.\u003cbr\u003e3. Sub-packaging may lead to the risk of ATP contamination, so avoid sub-packaging.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIt is suitable for activity detection of bioactive factors, large-scale anti-tumor drug screening, cell proliferation, cytotoxicity test, etc.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"50mL×2","offer_id":41293300596811,"sku":null,"price":900.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_d4ac30f8-afb3-4141-ba4f-27e51fec1c91.png?v=1770714915"},{"product_id":"mtt-cell-proliferation","title":"MTT Cell Proliferation and Cytotoxicity Assay Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Place the cell plate in the sterile operating table.\u003cbr\u003e2. Add 10% volume of MTT solvent to each well of the cell plate. [Preparation of MTT solution: Dissolve 25mg of MTT with 5ml of MTT solvent to prepare a 5mg\/ml MTT solution. It can be used after preparation, or stored directly at-20 ℃ in the dark from light, or stored at-20 ℃ in the dark from light after appropriately packaging as needed.]\u003cbr\u003e3. Put the cell plate back into the incubator and continue to incubate for 3-4 hours. When doing comparative experiments, the incubation time should be consistent.\u003cbr\u003e4. After incubation, take out the cell plate from the incubator and dissolve it according to the following method to produce MTT formazan crystals.\u003cbr\u003e(1) If it is an adherent cell, discard the culture medium. Add the same amount of Formazan Solvent as the initial volume. The volume of dissolved fluid may vary per well, but the total volume must be consistent for comparison.\u003cbr\u003e(2) If the removal of non-adherent cells or culture medium will cause the loss of MTT formazan, add the same amount of lysate as the initial volume directly to the culture medium.\u003cbr\u003e(3) Readings must be completed within 1 hour after addition of Formazan Solvent.\u003cbr\u003e5. Gentle shaking in a shaker can enhance dissolution. In some cases, particularly when the cell density is high, repeated pipetting is required to completely dissolve the MTT formazan crystals.\u003cbr\u003e6. Spectrophotometer to measure OD570 and reference wavelength OD690.\u003cbr\u003e7. The 96-well plate can be detected on a microplate reader equipped with a suitable filter.\u003cbr\u003e8. Other types of multi-well plates need to be spectrophotometrically detected with a cuvette or plate reader of appropriate size.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBeilstein\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMTT Cell Proliferation and Cytotoxicity Assay Kit,MTT kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eThe MTT assay, also known as MTT colorimetry, is a method to detect cell survival and growth. The detection principle is that succinate dehydrogenase in mitochondria of living cells can reduce exogenous MTT to water-insoluble blue-violet crystalline Formazan and deposit it in cells, while dead cells do not have this function. Within a certain range of cell numbers, the amount of MTT crystals formed is directly proportional to the number of cells. The greater the absorbance, the stronger the cell activity, indicating that the drug toxicity is less. This method has been widely used in the activity detection of some bioactive factors, large-scale anti-tumor drug screening, cytotoxicity test and tumor radiosensitivity determination. It is characterized by high sensitivity and economy.\u003c\/p\u003e\n\u003cdiv\u003eComponents: MTT (25mg), MTT solvent (5ml), Formazan solution (55ml)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003ePubChem CID\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Since a 96-well plate is used for detection, if the cell culture time is long, we must pay attention to the problem of evaporation. On the one hand, since the circle around the 96-well plate is the easiest to evaporate, you can discard the circle around the plate and add PBS, water or culture medium instead; On the other hand, a 96-well plate can be placed close to a water source in the incubator to alleviate evaporation.\u003cbr\u003e2. If the test compound used can react with MTT, centrifuge first and then discard the culture medium. Carefully flush it with PBS for 2 to 3 times, and then add the culture medium containing MTT.\u003cbr\u003e3. The production of Formazan is not only proportional to the number of living cells, but also affected by the action time. Therefore, when there are many samples, the measurement results may change with time.\u003cbr\u003e4. This product contains carcinogens, avoid contact with skin and eyes, please operate carefully. For your safety and health, please operate in a lab coat and disposable gloves.\u003cbr\u003e5. This product is only used for scientific research and cannot be used clinically or diagnostically.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStored at 2 ~ 8 ℃, protected from light, valid for 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"500T","offer_id":41518128595019,"sku":"abs50010-500T","price":100.0,"currency_code":"USD","in_stock":true}]},{"product_id":"celltiter-turbo-2-0-luminescentcell-viability-assay","title":"CTG-L™ 2D Luminescent Cell Viability Assay Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003e\u003cstrong\u003e1. Reagent preparation\u003c\/strong\u003e\u003cstrong\u003e：\u003cbr\u003e\u003c\/strong\u003e\u003cstrong\u003eReagent melt:\u003c\/strong\u003eThe reagents were removed the day before the experiment and left at 4 °C overnight to melt. The reagents can also be taken out on the day of the experiment and melted at room temperature, or melted in a 22 °C water bath, but\u003cstrong\u003eIt is necessary to note that the water temperature should not exceed\u003c\/strong\u003e\u003cstrong\u003e25\u003c\/strong\u003e\u003cstrong\u003e℃\u003c\/strong\u003e。\u003cbr\u003e\u003cstrong\u003eEquilibrate to room temperature:\u003c\/strong\u003eIf the reagent melts at non-room temperature, it can be placed in a 22 ℃ water bath before use to ensure that the reagent is equilibrated to room temperature before being used for testing.\u003cbr\u003eNote: Generally, it takes about 10 minutes for 5mL packaging; The 50 mL packaging takes about 20 minutes.\u003cbr\u003e\u003cstrong\u003eMix well:\u003c\/strong\u003eBefore use\u003cstrong\u003eGentle upside down\u003c\/strong\u003e\u003cstrong\u003e5\u003c\/strong\u003e\u003cstrong\u003eTime\u003c\/strong\u003eThe solution was mixed evenly.\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e2. Detection steps:\u003cbr\u003e\u003c\/strong\u003e1. Cell culture: Use a 96-well plate suitable for chemiluminescence detection, and inoculate 100μL cells per well (determine the initial cell density according to the culture time, and the number of cells per well should not exceed 100,000 during detection). At the same time, set a well of culture medium containing no cells as a negative control. The concentration gradient of the cells can also be set to obtain the best experimental results. 37 °C, 5% CO\u003csub\u003e2\u003c\/sub\u003eCulture the cells and treat the cells with dosing at the appropriate time as needed.\u003cbr\u003e2. (Optional) Preparation of ATP standard curve:\u003cbr\u003eThe prepared ATP standard solution was diluted with PBS to the appropriate concentration gradient, and 100 μL of the standard was added to each well of the 96-well plate.\u003cbr\u003e3. Cell viability detection\u003cbr\u003e(1) Thaw the frozen luminescence detection reagent, equilibrate it to room temperature (or 22 ℃ constant temperature water bath equilibrium), and take out the volume of detection reagent used for the current experiment\u003cbr\u003e(2) Take out the cell culture plate and balance it at room temperature for 10 minutes (or balance it in a constant temperature water bath at 22 ℃, the time should not be too long, try to control it within 30 minutes)\u003cbr\u003e(3) Add 100μL of detection reagent to each well of 96-well plate (due to the edge effect of the well, the luminescence signal may be unstable, so it is not recommended to plate at the edge)\u003cbr\u003e(4) Shake at room temperature for 2 minutes to promote cell lysis;\u003cbr\u003e(5) Place at room temperature for 10 minutes to stabilize the luminescent signal;\u003cbr\u003e(6) Use a multifunctional microplate reader for chemiluminescence detection. Set the corresponding parameters according to the requirements of the instrument. The detection time of each hole is generally 0.25-1s. The specific adjustments need to be made according to the detection sensitivity of the instrument;\u003cbr\u003e(7) Calculate the relative viability of cells according to the chemiluminescence reading, or calculate the ATP content according to the ATP standard curve to obtain the relative viability of cells.\u003cbr\u003eNote: The detection effect varies with different types of cells. For some cells with particularly high ATP content, the chemiluminescence reading may continue to increase when the number of cells reaches more than 100,000, but the linear relationship is lost.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eATP cell viability detection kit; 2D Cell Viability Detection Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2D Luminescent Cell Viability Assay uses ATP-dependent luciferase-catalyzed luciferin luminescence reaction to measure intracellular ATP content through chemiluminescence signal, so as to detect cell viability or quantitatively detect the number of viable cells. The detection has wide linear range, high sensitivity and good stability. In 96-well plates, there is a good linear relationship in the range of 100 to 100,000 cells, but the upper limit of the number of detected cells will be different. In addition, the operation is simple, the detection reagents provided in the kit are ready-to-use, with stable readings and fast detection speed. It only takes about 10 minutes to complete the detection, and there is no need to wash the cells, and there is no need to replace or remove the culture medium. Compared with other common cell viability measurement methods, such as Calcein-AT, CCK-8, etc., 2D Luminescent Cell Viability Assay is simpler and faster.  \u003cbr\u003eThe 2D Luminescent Cell Viability Assay has high detection sensitivity and wide linear range, and is compatible with small sample detection and high-throughput screening of large samples.  \u003cbr\u003e1. Convenient and fast: The detection reagents provided in the kit are ready-to-use, with stable readings and fast detection speed. It only takes about 10 minutes to complete the detection;  \u003cbr\u003e2. High sensitivity: can detect ATP with a minimum of 10nmol;  \u003cbr\u003e3. Wide linear range: In the 96-well plate, there is a good linear relationship in the range of 100 to 100,000 cells, but the upper limit of the number of detected cells will be different;  \u003cbr\u003e4. High throughput: It is compatible with the detection of a small amount of samples and the high throughput screening of a large number of samples.\u003cdiv\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20240312\/1e959b9d92ce44879f45c6ec48822124.png\" alt=\"\" width=\"571\" height=\"180\"\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003e\u003cstrong\u003e1, temperature:\u003c\/strong\u003eThe reagent contains luciferase, and repeated freezing and thawing will affect its activity. It is recommended to store at-20 ℃ in the dark from light after dispensing. Reagents and cell samples should be balanced to room temperature before use to avoid the influence of enzyme catalytic effect;\u003cbr\u003e\u003cstrong\u003e2. Chemical factors:\u003c\/strong\u003eThe reaction rate and luminescence intensity of luciferase are affected by the chemical environment. There are differences in luminescence intensity and attenuation rate among different types of media and serum. In addition, high drug content may interfere with the luciferase reaction, thus affecting the luminescence signal. It is recommended to set up cell culture medium control wells containing drug to eliminate solvent interference. If the culture medium has a great influence on the luminescence intensity, it can be removed before detection and washed once with PBS;\u003cbr\u003e\u003cstrong\u003e3. Light sensitivity:\u003c\/strong\u003eThis reagent is sensitive to light and will accelerate the attenuation of luminous intensity if exposed to light during storage. If the reagent is transferred from the original container, make sure to keep it protected from light;\u003cbr\u003e\u003cstrong\u003e4. ATP\u003c\/strong\u003e\u003cstrong\u003eContamination:\u003c\/strong\u003eIt is recommended to wear a mask and latex gloves during operation and avoid contact with surfaces and equipment that may be contaminated. Avoid inserting the tip of the gun tip into the vial multiple times during operation.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eInstructions\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003e\u003cstrong\u003eI. Reagent preparation \u003c\/strong\u003e\u003cstrong\u003e: \u003cbr\u003e\u003c\/strong\u003e\u003cstrong\u003eReagent melting: \u003c\/strong\u003eThe day before the experiment, the reagent was removed and placed at 4 ° C overnight to melt. The reagent can also be removed on the day of the experiment and melted at room temperature, or placed in a 22℃ water bath to melt, but \u003cstrong\u003eneed to note that the water temperature should not exceed \u003c\/strong\u003e\u003cstrong\u003e25\u003c\/strong\u003e\u003cstrong\u003e℃\u003c\/strong\u003e. \u003cbr\u003e\u003cstrong\u003eequilibrate to room temperature: \u003c\/strong\u003eIf the reagent melts under non-room temperature conditions, it can be placed in a 22℃ water bath before use to ensure that the reagent equilibrates to room temperature before being used for detection. \u003cbr\u003eNote: Generally, it takes about 10 minutes for 5mL packaging; 50mL packaging takes about 20min. \u003cbr\u003e\u003cstrong\u003eMix: \u003cstrong\u003egently invert \u003c\/strong\u003e\u003cstrong\u003e5 \u003c\/strong\u003e\u003cstrong\u003etimes \u003c\/strong\u003eto mix the solution well before use. \u003cbr\u003e\u003cstrong\u003eII. Detection steps: \u003cbr\u003e\u003c\/strong\u003e1. Cell culture: use a 96-well plate suitable for chemiluminescence detection, and inoculate 100\u0026amp;mu in each well; L cells (according to the culture time to determine the initial seeding cell density, the number of cells per well should not exceed 100 000), at the same time, set up the culture medium without cells as a negative control. The concentration gradient of cells can also be set to obtain the best experimental results. Cells were cultured at 37 ° C with 5% CO\u003csub\u003e2\u003c\/sub\u003eand treated with drugs at the appropriate time as needed. \u003cbr\u003e2. (Optional) Preparation of ATP standard curve: \u003cbr\u003eThe prepared ATP standard solution was diluted into an appropriate concentration gradient with PBS, and 100\u0026amp;mu was added to each well of a 96-well plate; L of the standard. \u003cbr\u003e3, cell viability detection \u003cbr\u003e(1) Thaw and thaw the luminescence method detection reagent, and balance to room temperature (or 22℃ constant temperature water bath equilibrium) \u003cbr\u003e(2) Remove the cell culture plate, room temperature equilibrium for 10 minutes (or 22℃ constant temperature water bath equilibrium, the time should not be too long, Try to control within 30 minutes) \u003cbr\u003e(3) Add 100\u0026amp;mu to each well of 96-well plate; L detection reagent (due to the edge effect of the well, it may cause the luminescence signal to be unstable, and it is not recommended to spread the plate at the edge) \u003cbr\u003e(4) shake at room temperature for 2 minutes to promote the lysis of cells; \u003cbr\u003e(5) place at room temperature for 10 minutes to make the luminescence signal tend to be stable; \u003cbr\u003e(6) chemiluminescence detection was performed using a multifunctional microplate reader. Set the corresponding parameters according to the requirements of the instrument. The detection time of each well is generally 0.25-1s, and the specific need to be adjusted appropriately according to the detection sensitivity of the instrument. \u003cbr\u003e(7) Relative cell viability was calculated according to the chemiluminescence reading, or ATP content was calculated according to the ATP standard curve to obtain the relative cell viability. \u003cbr\u003eNote: The detection effect varies with the type of cells. For some cells with particularly high ATP content, the chemiluminescence reading may continue to increase when the number of cells reaches 100,000, but the linear relationship may be lost. \u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eStore at-20 °C protected from light. In order to ensure the best performance, it is recommended to place it at-70 ℃ for long-term storage.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIt is suitable for activity detection of bioactive factors, large-scale anti-tumor drug screening, cell proliferation, cytotoxicity test, etc.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"10mL","offer_id":41820546269259,"sku":null,"price":40.0,"currency_code":"USD","in_stock":true},{"title":"2×50mL","offer_id":41518140915787,"sku":null,"price":159.0,"currency_code":"USD","in_stock":true},{"title":"100mL","offer_id":41820546302027,"sku":null,"price":159.0,"currency_code":"USD","in_stock":true},{"title":"10×100mL","offer_id":41820546334795,"sku":null,"price":1200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_3d9376a5-8173-4866-a462-35969099c0c7.png?v=1770714806"},{"product_id":"t-cell-signaling-miniab-kit","title":"T Cell Signaling MiniAb Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eWB\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHu\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003ePBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e12 months from date of receipt \/ reconstitution, -20 °C as supplied\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1Kit","offer_id":41518765736011,"sku":"S0M1049-1Kit","price":660.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_e74ca3fb-6b23-4432-a21a-1b4c3b70d745.png?v=1788508907"},{"product_id":"tcellxviva-mouse-t-cell-activation-kit","title":"CellXViva Mouse T cell Activation Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eMouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReconstitution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cfont\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eIL-2 Protein, Mouse: \u003cspan style=\"color: rgb(73, 80, 87);font-size: 14.6667px\"\u003eReconstitute at 0.1-1 mg\/ml according to the size in ultrapure water after rapid centrifugation.\u003c\/span\u003e\u003c\/span\u003e\u003c\/font\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan style=\"color: rgb(73, 80, 87);font-size: 13px\"\u003e-20 to -80℃. Please avoid repeated freeze-thaw cycles.\u003cbr\u003e\u003cbr\u003eValid for 12 months from the date of receipt.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;width:504.4pt;\" border=\"0\" cellspacing=\"0\" cellpadding=\"0\" width=\"673\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height:20.15pt;\"\u003e\n\u003ctd style=\"background-color:white;border:solid black 1.0pt;height:20.15pt;padding:0cm 5.4pt 0cm 5.4pt;width:172.4pt;\" width=\"230\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eContain\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:white;border-left:none;border:solid black 1.0pt;height:20.15pt;padding:0cm 5.4pt 0cm 5.4pt;width:82.65pt;\" width=\"110\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eFormulation\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:white;border-left:none;border:solid black 1.0pt;height:20.15pt;padding:0cm 5.4pt 0cm 5.4pt;width:67.65pt;\" width=\"90\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eReference dosage\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:white;border-left:none;border:solid black 1.0pt;height:20.15pt;padding:0cm 5.4pt 0cm 5.4pt;width:67.6pt;\" width=\"90\" nowrap\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eS Size\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e(200mL system)\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"background-color:white;border-left:none;border:solid black 1.0pt;height:20.15pt;padding:0cm 5.4pt 0cm 5.4pt;width:61.85pt;\" width=\"82\" nowrap\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eM Size\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e(1L system)\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"background-color:white;border-left:none;border:solid black 1.0pt;height:20.15pt;padding:0cm 5.4pt 0cm 5.4pt;width:52.25pt;\" width=\"70\" nowrap\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eL Size\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e(2L system)\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:20.15pt;\"\u003e\n\u003ctd style=\"background-color:white;border-top:none;border:solid black 1.0pt;height:20.15pt;padding:0cm 5.4pt 0cm 5.4pt;width:172.4pt;\" width=\"230\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eIL-2 Protein, Mouse\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:white;border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:20.15pt;padding:0cm 5.4pt 0cm 5.4pt;width:82.65pt;\" width=\"110\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eLyophilized Powder\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:white;border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:20.15pt;padding:0cm 5.4pt 0cm 5.4pt;width:67.65pt;\" width=\"90\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10 ng\/mL\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:white;border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:20.15pt;padding:0cm 5.4pt 0cm 5.4pt;width:67.6pt;\" width=\"90\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e5ug\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:white;border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:20.15pt;padding:0cm 5.4pt 0cm 5.4pt;width:61.85pt;\" width=\"82\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10ug\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:white;border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:20.15pt;padding:0cm 5.4pt 0cm 5.4pt;width:52.25pt;\" width=\"70\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e20ug\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:20.15pt;\"\u003e\n\u003ctd style=\"background-color:white;border-top:none;border:solid black 1.0pt;height:20.15pt;padding:0cm 5.4pt 0cm 5.4pt;width:172.4pt;\" width=\"230\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eNA\/LE Rat anti-mouse CD3 Recombinant mAb\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:white;border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:20.15pt;padding:0cm 5.4pt 0cm 5.4pt;width:82.65pt;\" width=\"110\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eLiquid\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:white;border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:20.15pt;padding:0cm 5.4pt 0cm 5.4pt;width:67.65pt;\" width=\"90\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10 µg\/mL\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:white;border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:20.15pt;padding:0cm 5.4pt 0cm 5.4pt;width:67.6pt;\" width=\"90\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2mg\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:white;border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:20.15pt;padding:0cm 5.4pt 0cm 5.4pt;width:61.85pt;\" width=\"82\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10mg\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:white;border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:20.15pt;padding:0cm 5.4pt 0cm 5.4pt;width:52.25pt;\" width=\"70\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e20mg\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:20.15pt;\"\u003e\n\u003ctd style=\"background-color:white;border-top:none;border:solid black 1.0pt;height:20.15pt;padding:0cm 5.4pt 0cm 5.4pt;width:172.4pt;\" width=\"230\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eNA\/LE Syrian Hamster anti-mouse CD28 mAb\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:white;border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:20.15pt;padding:0cm 5.4pt 0cm 5.4pt;width:82.65pt;\" width=\"110\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eLiquid\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:white;border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:20.15pt;padding:0cm 5.4pt 0cm 5.4pt;width:67.65pt;\" width=\"90\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2 µg\/mL\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:white;border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:20.15pt;padding:0cm 5.4pt 0cm 5.4pt;width:67.6pt;\" width=\"90\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e0.5mg\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:white;border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:20.15pt;padding:0cm 5.4pt 0cm 5.4pt;width:61.85pt;\" width=\"82\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2mg\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:white;border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:20.15pt;padding:0cm 5.4pt 0cm 5.4pt;width:52.25pt;\" width=\"70\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e4mg\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e \u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003eComplete medium formulation for reference:\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;width:231.6pt;\" border=\"0\" cellspacing=\"0\" cellpadding=\"0\" width=\"309\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height:11.55pt;\"\u003e\n\u003ctd style=\"border:solid black 1.0pt;height:11.55pt;padding:0cm 5.4pt 0cm 5.4pt;width:140.7pt;\" width=\"188\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;\"\u003eComponent\u003c\/span\u003e\u003cspan style=\"font-family:宋体;\" lang=\"EN-US\"\u003e  \u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid black 1.0pt;height:11.55pt;padding:0cm 5.4pt 0cm 5.4pt;width:90.9pt;\" width=\"121\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eFinal Concentration\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:23.15pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid black 1.0pt;height:23.15pt;padding:0cm 5.4pt 0cm 5.4pt;width:140.7pt;\" width=\"188\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;' lang=\"EN-US\"\u003eRPMI-1640            \u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:23.15pt;padding:0cm 5.4pt 0cm 5.4pt;width:90.9pt;\" width=\"121\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e90%\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:11.55pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid black 1.0pt;height:11.55pt;padding:0cm 5.4pt 0cm 5.4pt;width:140.7pt;\" width=\"188\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;\"\u003eFetal Bovine Serum\u003c\/span\u003e\u003cspan style=\"font-family:宋体;\" lang=\"EN-US\"\u003e            \u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:11.55pt;padding:0cm 5.4pt 0cm 5.4pt;width:90.9pt;\" width=\"121\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10%\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:22.75pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid black 1.0pt;height:22.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:140.7pt;\" width=\"188\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;\"\u003eSodium Pyruvate\u003c\/span\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;' lang=\"EN-US\"\u003e100x        \u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:22.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:90.9pt;\" width=\"121\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1x\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:23.15pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid black 1.0pt;height:23.15pt;padding:0cm 5.4pt 0cm 5.4pt;width:140.7pt;\" width=\"188\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;' lang=\"EN-US\"\u003eL-\u003c\/span\u003e\u003cspan style=\"font-family:宋体;\"\u003eGlutamine\u003c\/span\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;' lang=\"EN-US\"\u003e100x      \u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:23.15pt;padding:0cm 5.4pt 0cm 5.4pt;width:90.9pt;\" width=\"121\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1x\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:11.55pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid black 1.0pt;height:11.55pt;padding:0cm 5.4pt 0cm 5.4pt;width:140.7pt;\" width=\"188\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;' lang=\"EN-US\"\u003eHEPES                               \u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:11.55pt;padding:0cm 5.4pt 0cm 5.4pt;width:90.9pt;\" width=\"121\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1x\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:22.75pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid black 1.0pt;height:22.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:140.7pt;\" width=\"188\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;\"\u003ePenicillin\u003c\/span\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;' lang=\"EN-US\"\u003e-\u003c\/span\u003e\u003cspan style=\"font-family:宋体;\"\u003eStreptomycin\u003c\/span\u003e\u003cspan style=\"font-family:宋体;\" lang=\"EN-US\"\u003e               \u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:22.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:90.9pt;\" width=\"121\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1x\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:22.75pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid black 1.0pt;height:22.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:140.7pt;\" width=\"188\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;\"\u003eβ\u003c\/span\u003e\u003cspan style='font-family:\"Calibri\",sans-serif;' lang=\"EN-US\"\u003e-\u003c\/span\u003e\u003cspan style=\"font-family:宋体;\"\u003eMercaptoethanol\u003c\/span\u003e\u003cspan style=\"font-family:宋体;\" lang=\"EN-US\"\u003e           \u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:22.75pt;padding:0cm 5.4pt 0cm 5.4pt;width:90.9pt;\" width=\"121\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e55 \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eμ\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eM\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cfont\u003e\u003cspan style=\"font-size:10.5000pt;\"\u003e\u003cstrong\u003eProtocol 1: Anti-Mouse CD3 Monoclonal Antibody Coating Method\u003c\/strong\u003e\u003c\/span\u003e\u003c\/font\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cfont\u003e\u003cspan style=\"font-size:10.5000pt;\"\u003e1.  Dilute NA\/LE\u003c\/span\u003e\u003c\/font\u003e\u003cspan style=\"font-size:10.5000pt;\"\u003e \u003c\/span\u003e\u003cfont\u003e\u003cspan style=\"font-size:10.5000pt;\"\u003eRat anti-mouse CD3 Recombinant mAb into PBS to a final concentration of 10 µg\/mL.\u003c\/span\u003e\u003c\/font\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cfont\u003e\u003cspan style=\"font-size:10.5000pt;\"\u003e2. Add the diluted NA\/LE\u003c\/span\u003e\u003c\/font\u003e\u003cspan style=\"font-size:10.5000pt;\"\u003e \u003c\/span\u003e\u003cfont\u003e\u003cspan style=\"font-size:10.5000pt;\"\u003eRat anti-mouse CD3 Recombinant mAb to a 24-well plate at 1 mL per well for coating.\u003c\/span\u003e\u003c\/font\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cfont\u003e\u003cspan style=\"font-size:10.5000pt;\"\u003e2. Incubate for 2 hours at 37°C with 5% CO₂, or overnight at 4°C.\u003c\/span\u003e\u003c\/font\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cfont\u003e\u003cspan style=\"font-size:10.5000pt;\"\u003e4. Remove the NA\/LE\u003c\/span\u003e\u003c\/font\u003e\u003cspan style=\"font-size:10.5000pt;\"\u003e \u003c\/span\u003e\u003cfont\u003e\u003cspan style=\"font-size:10.5000pt;\"\u003eRat anti-mouse CD3 Recombinant mAb from the 24-well plate.\u003c\/span\u003e\u003c\/font\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cfont\u003e\u003cspan style=\"font-size:10.5000pt;\"\u003e5. Prepare a single-cell suspension from mouse spleen. For T cell activation, cells should be resuspended in complete medium at a concentration of (1-3)×10⁶ cells\/mL. For a 24-well plate, a concentration of 1×10⁶ cells\/mL is recommended.\u003c\/span\u003e\u003c\/font\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cfont\u003e\u003cspan style=\"font-size:10.5000pt;\"\u003e6. Prepare fully supplemented T cell medium in a V-bottom tube, and add the following cytokines and antibodies to the cell suspension from step 6:\u003c\/span\u003e\u003c\/font\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt;\"\u003e   \u003c\/span\u003e\u003cfont\u003e\u003cspan style=\"font-size:10.5000pt;\"\u003e- 2µg\/mL NA\/LE\u003c\/span\u003e\u003c\/font\u003e\u003cspan style=\"font-size:10.5000pt;\"\u003e \u003c\/span\u003e\u003cfont\u003e\u003cspan style=\"font-size:10.5000pt;\"\u003eSyrian Hamster anti-mouse CD28 mAb\u003c\/span\u003e\u003c\/font\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt;\"\u003e   \u003c\/span\u003e\u003cfont\u003e\u003cspan style=\"font-size:10.5000pt;\"\u003e- 10ng\/mL\u003c\/span\u003e\u003c\/font\u003e\u003cspan style=\"font-size:10.5000pt;\"\u003e \u003c\/span\u003e\u003cfont\u003e\u003cspan style=\"font-size:10.5000pt;\"\u003eIL-2 Protein, Mouse\u003c\/span\u003e\u003c\/font\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cfont\u003e\u003cspan style=\"font-size:10.5000pt;\"\u003e7. Transfer the required amount of cells to an appropriate cell culture plate to a final density of (1-1.5)×10⁶ cells\/mL\/cm².\u003c\/span\u003e\u003c\/font\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cfont\u003e\u003cspan style=\"font-size:10.5000pt;\"\u003e8. Incubate for 2 days at 37°C with 5% CO₂.\u003c\/span\u003e\u003c\/font\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cfont\u003e\u003cspan style=\"font-size:10.5000pt;\"\u003e9. After 2 days of culture, check cell health and record images.\u003c\/span\u003e\u003c\/font\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cfont\u003e\u003cspan style=\"font-size:10.5000pt;\"\u003e10. Centrifuge the cell suspension at 400 × g for 5 minutes, discard the supernatant. Wash the cell pellet with PBS.\u003c\/span\u003e\u003c\/font\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cfont\u003e\u003cspan style=\"font-size:10.5000pt;\"\u003e11. Centrifuge the cell suspension at 400 × g for 5 minutes, discard the supernatant. Resuspend the cells in 0.5 mL of 1% Bovine Serum Albumin (BSA) and count the cells. Assess cell viability, requiring \u0026gt;95% viability.\u003c\/span\u003e\u003c\/font\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cfont\u003e\u003cspan style=\"font-size:10.5000pt;\"\u003e12. Block 1×10⁶ cells with 10 µg of mouse IgG antibody. Incubate on ice for 30 minutes.\u003c\/span\u003e\u003c\/font\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cfont\u003e\u003cspan style=\"font-size:10.5000pt;\"\u003e13. Add FITC Rat anti-Mouse CD25 Antibody (S-R441) (S0B5215) according to the instructions, and incubate at 4°C for 30 minutes.\u003c\/span\u003e\u003c\/font\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cfont\u003e\u003cspan style=\"font-size:10.5000pt;\"\u003e14. Centrifuge the cell suspension at 400 × g for 5 minutes, and wash the cell pellet twice with PBS containing 1% BSA.\u003c\/span\u003e\u003c\/font\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cfont\u003e\u003cspan style=\"font-size:10.5000pt;\"\u003e15. Resuspend the cell pellet in 200 µL PBS for flow cytometry analysis (requires \u0026gt;10,000 cells).\u003c\/span\u003e\u003c\/font\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"S","offer_id":41519877455947,"sku":"UA090032-S","price":630.0,"currency_code":"USD","in_stock":true},{"title":"M","offer_id":43085222412363,"sku":"UA090032-M","price":1100.0,"currency_code":"USD","in_stock":true},{"title":"L","offer_id":41519877488715,"sku":"UA090032-L","price":2490.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/074cd72e-def3-45cd-a137-3926c2db4ea5.png?v=1787997608"},{"product_id":"tcellxviva-human-t-cell-activation-kit","title":"CellXViva Human T cell Activation Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReconstitution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan style=\"color: rgb(51, 51, 51);font-size: 14px\"\u003eIL-2 Protein, Human: \u003c\/span\u003e\u003cspan style=\"color: rgb(73, 80, 87);font-size: 14.6667px\"\u003eReconstitute at 0.1-1 mg\/ml according to the size in ultrapure water after rapid centrifugation.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e-20to -80℃. Please avoid repeated freeze-thaw cycles.\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#333333\"\u003eValid for 12 months from the date of receipt.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;width:431.5pt;\" border=\"0\" cellspacing=\"0\" cellpadding=\"0\" width=\"575\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height:14.0pt;\"\u003e\n\u003ctd style=\"border:solid black 1.0pt;height:14.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:184.5pt;\" width=\"246\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eContain\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid black 1.0pt;height:14.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:83.9pt;\" width=\"112\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eFormulation\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid black 1.0pt;height:14.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:42.65pt;\" width=\"57\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eReference dosage\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid black 1.0pt;height:14.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:40.15pt;\" width=\"54\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eS Size(200mL)\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid black 1.0pt;height:14.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:40.15pt;\" width=\"54\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eM Size(1L)\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid black 1.0pt;height:14.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:40.15pt;\" width=\"54\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eL Size(2L)\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:14.0pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid black 1.0pt;height:14.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:184.5pt;\" width=\"246\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eIL-2 Protein, Human\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:14.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:83.9pt;\" width=\"112\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eLyophilized Powder\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:14.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:42.65pt;\" width=\"57\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10 ng\/mL\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:14.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:40.15pt;\" width=\"54\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e5ug\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:14.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:40.15pt;\" width=\"54\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10ug\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:14.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:40.15pt;\" width=\"54\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e20ug\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:14.0pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid black 1.0pt;height:14.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:184.5pt;\" width=\"246\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eNA\/LE Mouse anti-human CD3 mAb\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:14.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:83.9pt;\" width=\"112\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eLiquid\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:14.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:42.65pt;\" width=\"57\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e5 µg\/mL\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:14.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:40.15pt;\" width=\"54\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1mg\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:14.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:40.15pt;\" width=\"54\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e5mg\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:14.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:40.15pt;\" width=\"54\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10mg\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:14.0pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid black 1.0pt;height:14.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:184.5pt;\" width=\"246\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eNA\/LE Mouse anti-human CD28 Recombinant mAb\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:14.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:83.9pt;\" width=\"112\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eLiquid\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:14.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:42.65pt;\" width=\"57\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e5 µg\/mL\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:14.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:40.15pt;\" width=\"54\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1mg\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:14.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:40.15pt;\" width=\"54\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e5mg\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:14.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:40.15pt;\" width=\"54\" nowrap\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10mg\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e \u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cb\u003e\u003cspan style=\"font-weight:bold;font-size:10.5000pt\"\u003eProtocol 1: \u003cfont\u003eanti\u003c\/font\u003e-Human \u003cfont\u003eCD\u003c\/font\u003e3 mAb immobilized Method\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e1. \u003cfont\u003eD\u003c\/font\u003eilute NA\/LE Mouse anti-Human CD3 mAb into a final concentration of 5µg\/mL in PBS.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e2. Immobilize NA\/LE Mouse anti-Human CD3 mAb by adding 1mL\u003cfont\u003e\/\u003c\/font\u003ewell diluted mAb into 24-well plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e3. \u003cfont\u003eI\u003c\/font\u003encubate at 37 °C and 5% CO\u003cfont\u003e₂\u003c\/font\u003e for 2h, or 4°C overnight.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e4. Drain NA\/LE Mouse anti-Human CD3 mAb \u003cfont\u003efrom\u003c\/font\u003e 24-well plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e5. Preparation of single-cell suspension from human PBMC. For T cell \u003cfont\u003eactivation,\u003c\/font\u003e the cells should be resuspended in complete medium at \u003cfont\u003e(\u003c\/font\u003e1-3)×10\u003cfont\u003e⁶\u003c\/font\u003ecells\/mL. 1×10\u003cfont\u003e⁶i\u003c\/font\u003es recommended for 24-well plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e6. Prepare fully supplemented T cell medium \u003cfont\u003ein\u003c\/font\u003e \u003cfont\u003eV-shape\u003c\/font\u003ed \u003cfont\u003etube\u003c\/font\u003e by adding cytokines and antibodies to the cell suspension from step 5 as follows:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003el 5µg\/mL NA\/LE Mouse anti-Human CD28 mAb\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003el \u003cfont\u003e1\u003c\/font\u003e0ng\/mL IL-2 Protein, Human\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e7. Transfer the desired amount of cells to a suitable cell culture plate to a final density of (1-1.5)×10\u003cfont\u003e⁶\u003c\/font\u003e cells\/mL\/cm².\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e8. Incubate at 37 °C and 5% CO\u003cfont\u003e₂\u003c\/font\u003e for 2 days.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e9. After 2 days of cultivation, examine for cell healthiness and record image. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e10. Centrifuge cell suspension at 400×g for 5 min. Discard medium supernatant. Wash cell pellet in PBS.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e11. Centrifuge cell suspension at 400×g for 5 minutes. Discard supernatant. \u003cfont\u003eR\u003c\/font\u003eesuspend cell in 0.5mL 1% BSA. Determine cell number. Examine for cell viability, \u0026gt;95% viability is required.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e12. Block 1×10\u003cfont\u003e⁶\u003c\/font\u003ecells in 10µg Human IgG antibody. Incubate 30min in ice bath.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e13. \u003cfont\u003eA\u003c\/font\u003edd Brilliant Violet 421TM anti-Human CD25 antibody (302630) and Alexa Fluor® 488 anti-human CD69 Antibody (310916) guided by the manufacture manual, incubate 30min at 4 \u003cfont\u003e°C\u003c\/font\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e14. Centrifuge cell suspension at 400×g for 5 minutes. \u003cfont\u003eW\u003c\/font\u003eash cell pellet in 1% BSA containing PBS twice.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e15. Resuspend cell pellet in a 200µl PBS for analysis by flow cytometry (\u0026gt;10000 cells required). \u003c\/span\u003e\u003c\/p\u003e\n\u003ch1\u003e\u003cb\u003e\u003cspan style=\"font-weight:bold;font-size:10.5000pt\"\u003eProtocol 2: \u003cfont\u003eanti\u003c\/font\u003e-Human \u003cfont\u003eCD\u003c\/font\u003e3 mAb \u003cfont\u003emobilized\u003c\/font\u003e Method\u003c\/span\u003e\u003c\/b\u003e\u003c\/h1\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e1. Preparation of single-cell suspension from Human PBMC. For T cell \u003cfont\u003eactivation,\u003c\/font\u003e the cells should be resuspended in complete medium at (1-3)×10\u003cfont\u003e⁶\u003c\/font\u003ecells\/mL. 1×10\u003cfont\u003e⁶i\u003c\/font\u003es recommended for 24-well plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e2. Prepare fully supplemented T cell medium \u003cfont\u003ein\u003c\/font\u003e \u003cfont\u003eV-shape\u003c\/font\u003ed \u003cfont\u003etube\u003c\/font\u003e by adding cytokines and antibodies to the cell suspension from step 2 as follows:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003el 5µg\/mL NA\/LE Mouse anti-Human CD3 mAb\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003el 5µg\/mL NA\/LE Mouse anti-Human CD28 mAb\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003el \u003cfont\u003e1\u003c\/font\u003e0ng\/mL IL-2 Protein, Human\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e3. Transfer the desired amount of cells to a suitable cell culture plate to a final density of (1-1.5)×10\u003cfont\u003e⁶\u003c\/font\u003e cells\/mL\/cm².\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e4. Incubate at 37 °C and 5% CO\u003cfont\u003e₂\u003c\/font\u003e for 2 days.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e5. After 2 days of cultivation, examine for cell healthiness and record image. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e6. Centrifuge cell suspension at 400×g for 5 min. Discard medium supernatant. Wash cell pellet in PBS.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e7. Centrifuge cell suspension at 400×g for 5 minutes. Discard supernatant. \u003cfont\u003eR\u003c\/font\u003eesuspend cell in 0.5mL 1% BSA. Determine cell number. Examine for cell viability, \u0026gt;95% viability is required.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e8. Block 1×10\u003cfont\u003e⁶\u003c\/font\u003ecells in 10µg Human IgG antibody. Incubate 30min in ice bath.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e9. \u003cfont\u003eA\u003c\/font\u003edd Brilliant Violet 421TM anti-Human CD25 antibody (302630) and Alexa Fluor® 488 anti-human CD69 Antibody (310916) guided by the manufacture manual, incubate 30min at 4 \u003cfont\u003e°C\u003c\/font\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e10. Centrifuge cell suspension at 400×g for 5 minutes. \u003cfont\u003eW\u003c\/font\u003eash cell pellet in 1% BSA containing PBS twice.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e11. Resuspend cell pellet in a 200µl PBS for analysis by flow cytometry (\u0026gt;10000 cells required).\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"S","offer_id":41519877587019,"sku":"UA090033-S","price":450.0,"currency_code":"USD","in_stock":true},{"title":"L","offer_id":41519877619787,"sku":"UA090033-L","price":1435.0,"currency_code":"USD","in_stock":true},{"title":"M","offer_id":43085293420619,"sku":"UA090033-M","price":1100.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/190ec99a-898b-4e61-8e6a-d227c4e7d1c9.png?v=1788001232"},{"product_id":"cytotoxic-t-cell-and-natural-killer-nk-cell-cd8-granzyme-b-antibody-panel","title":"Cytotoxic T cell and natural killer (NK) cell (CD8α, Granzyme B) Antibody Panel","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAntibody Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eRecombinant mAb\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003emIHC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHu\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003ePBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e12 months from date of receipt \/ reconstitution, -20 °C as supplied\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eDilution\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 60%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eapplication\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003edilution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003especies\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003emIHC\u003c\/td\u003e\n\u003ctd\u003e1:200-1:500\u003c\/td\u003e\n\u003ctd\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003eCytotoxic T cell and natural killer (NK) cell (CD8α, Granzyme B) Antibody Panel contains 2 recombinant monoclonal antibodies against CD8α, Granzyme B. They are provided as a sampler panel. \u003c\/p\u003e\n\u003cp\u003ePanel contains: \u003c\/p\u003e\n\u003cp\u003e- Granzyme B Recombinant Rabbit mAb (S-357-54) S0B0359\u003c\/p\u003e\n\u003cp\u003e- CD8α Recombinant Mouse mAb (SDT-126-8) S0B2148\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"25μl","offer_id":41677635092555,"sku":"S0C2005-25μl","price":200.0,"currency_code":"USD","in_stock":true},{"title":"100μl","offer_id":41677635125323,"sku":"S0C2005-100μl","price":670.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/931138bb1bf844fda8d45844fd7cce4d.jpg?v=1787612548"},{"product_id":"pd-l1-cytotoxic-t-cell-and-helper-t-cell-pd-l1-cd8-cd4-antibody-panel","title":"PD-L1, Cytotoxic T cell and Helper T cell (PD-L1, CD8α, CD4) Antibody Panel","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAntibody Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eRecombinant mAb\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003emIHC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHu\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003ePBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e12 months from date of receipt \/ reconstitution, -20 °C as supplied\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eDilution\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 60%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eapplication\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003edilution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003especies\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003emIHC\u003c\/td\u003e\n\u003ctd\u003e1:200-1:1000\u003c\/td\u003e\n\u003ctd\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-size:12.0000pt\"\u003e\u003cfont\u003ePD-L1, Cytotoxic T cell and Helper T cell (PD-L1, CD8α, CD4) Antibody Panel contains 3 recombinant monoclonal antibodies against PD-L1, CD8α, CD4. They are provided as a sampler panel. \u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:12.0000pt\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:12.0000pt\"\u003e\u003cfont\u003ePanel contains: \u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:12.0000pt\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:12.0000pt\"\u003e\u003cfont\u003e- S-RMab® PD-L1 Recombinant Rabbit mAb (SDT-R017) S0B2134\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:12.0000pt\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:12.0000pt\"\u003e\u003cfont\u003e- CD8α Recombinant Mouse mAb (SDT-126-8) S0B2148\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:12.0000pt\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:12.0000pt\"\u003e\u003cfont\u003e- CD4 Recombinant Rabbit mAb (SDT-R163) S0B2179\u003c\/font\u003e\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"25μl","offer_id":41677645152331,"sku":"S0C2009-25μl","price":300.0,"currency_code":"USD","in_stock":true},{"title":"100μl","offer_id":41677645185099,"sku":"S0C2009-100μl","price":1000.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/be761a5001d7448ebb7a80fa9d380bf4.jpg?v=1787619743"},{"product_id":"b7-h3-t-cell-cytotoxic-t-cell-and-helper-t-cell-b7-h3-cd3-epsilon-cd8-cd4-antibody-panel","title":"B7-H3, T cell, Cytotoxic T cell and Helper T cell (B7-H3, CD3 epsilon, CD8α, CD4) Antibody Panel","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAntibody Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eRecombinant mAb\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003emIHC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHu\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003ePBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e12 months from date of receipt \/ reconstitution, -20°C as supplied\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eDilution\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 60%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eapplication\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003edilution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003especies\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003emIHC\u003c\/td\u003e\n\u003ctd\u003e1:200-1:1000\u003c\/td\u003e\n\u003ctd\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-size:12.0000pt\"\u003e\u003cfont\u003eB7-H3, T cell, Cytotoxic T cell and Helper T cell (B7-H3, CD3 epsilon, CD8α, CD4) Antibody Panel contains 4 recombinant monoclonal antibodies against B7-H3, CD3 epsilon, CD8α, CD4. They are provided as a sampler panel.  \u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:12.0000pt\"\u003e\u003cfont\u003ePanel contains:  \u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:12.0000pt\"\u003e\u003cfont\u003e- CD4 Recombinant Rabbit mAb (SDT-R163) S0B2179 \u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:12.0000pt\"\u003e\u003cfont\u003e- CD8α Recombinant Mouse mAb (SDT-126-8) S0B2148 \u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:12.0000pt\"\u003e\u003cfont\u003e- CD3 epsilon Recombinant Rabbit mAb (SDT-241-49) S0B2132 - B7-H3 Recombinant Rabbit mAb (SDT-249-26) S0B2244\u003c\/font\u003e\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"25μl","offer_id":41677661536331,"sku":"S0C201-25μl","price":400.0,"currency_code":"USD","in_stock":true},{"title":"100μl","offer_id":41677661569099,"sku":"S0C201-100μl","price":1335.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/75dfdae789b5475f83d88022ceb2a864.jpg?v=1787684615"},{"product_id":"pd-1-t-cell-cytotoxic-t-cell-and-macrophage-marker-pd-1-cd3-epsilon-cd8-cd68-antibody-panel","title":"PD-1, T cell, Cytotoxic T cell and Macrophage Marker (PD-1, CD3 epsilon, CD8α, CD68) Antibody Panel","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAntibody Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eRecombinant mAb\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003emIHC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHu\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003ePBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e12 months from date of receipt \/ reconstitution, -20 °C as supplied\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eDilution\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 60%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eapplication\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003edilution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003especies\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003emIHC\u003c\/td\u003e\n\u003ctd\u003e1:200-1:1000\u003c\/td\u003e\n\u003ctd\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003ePD-1, T cell, Cytotoxic T cell and Macrophage Marker (PD-1, CD3 epsilon, CD8α, CD68) Antibody Panel contains 4 recombinant monoclonal antibodies against PD-1, CD3 epsilon, CD8α, CD68. They are provided as a sampler panel. \u003cbr\u003e Panel contains: \u003cbr\u003e - S-RMab® PD-1 Recombinant Mouse mAb (SDT-R143) S0B2187 \u003cbr\u003e - CD3 epsilon Recombinant Rabbit mAb (SDT-241-49) S0B2132 \u003cbr\u003e - CD8α Recombinant Mouse mAb (SDT-126-8) S0B2148 \u003cbr\u003e - S-RMab® CD68 Recombinant Rabbit mAb (SDT-R025) S0B2064\u003cbr\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"25μl","offer_id":41677695680587,"sku":"S0C2023-25μl","price":400.0,"currency_code":"USD","in_stock":true},{"title":"100μl","offer_id":41677695713355,"sku":"S0C2023-100μl","price":1335.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/e5f99a787aba4a4b9c4a18185b9b6377.jpg?v=1787918514"},{"product_id":"pd-l1-regulatory-t-cell-cytotoxic-t-cell-and-macrophage-marker-pd-l1-foxp3-cd8-cd68-cd163-antibody-panel","title":"PD-L1, Regulatory T cell, Cytotoxic T cell and Macrophage Marker (PD-L1, FOXP3, CD8α, CD68, CD163) Antibody Panel","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAntibody Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eRecombinant mAb\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003emIHC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHu\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003emg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConjugation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003ePBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e12 months from date of receipt \/ reconstitution, -20 °C as supplied\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eDilution\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 60%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eapplication\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003edilution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003especies\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003emIHC\u003c\/td\u003e\n\u003ctd\u003e1:100-1:2000\u003c\/td\u003e\n\u003ctd\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003ePD-L1, Regulatory T cell, Cytotoxic T cell and Macrophage Marker (PD-L1, FOXP3, CD8α, CD68, CD163) Antibody Panel contains 5 recombinant monoclonal antibodies against PD-L1, FOXP3, CD8α, CD68 and CD163. They are provided as a sampler panel. Panel contains:- PD-L1 Recombinant Rabbit mAb (SDT-R017) S0B2134- S-RMab® FOXP3 Recombinant Mouse mAb (SDT-R149) S0B2193- CD8α Recombinant Mouse mAb (SDT-126-8) S0B2148- S-RMab® CD68 Recombinant Rabbit mAb (SDT-R025) S0B2064- S-RMab® CD163 Recombinant Rabbit mAb (SDT-R164) S0B2194\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"25μl","offer_id":41677711704139,"sku":"S0C2030-25μl","price":500.0,"currency_code":"USD","in_stock":true},{"title":"100μl","offer_id":41677711736907,"sku":"S0C2030-100μl","price":1670.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/ba0962722c63414db0cecd0ea938cfe3.jpg?v=1787929241"},{"product_id":"e-coli-host-cell-proteins-onestep-elisa-kit","title":"E. coli Host Cell Proteins OneStep ELISA Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAntigen\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHost Cell Proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eE. coli HCP、HCP\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eImmunogen\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eRecombinant Protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAntibody Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003epAb\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eOthers\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurification\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eProtein A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e2 to 8 °C as supplied.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eKit\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eIntra-assay: 4.2%\u003cfont\u003e；\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eInter-assay: \u003cfont\u003e5\u003c\/font\u003e.7%\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSample type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eCell culture supernatant\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAssay type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eSandwich (quantitative)\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e\u003cfont\u003e0.1\u003c\/font\u003e83 ng\/ml\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eRange\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e0.39 ng\/mL – 25 ng\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eCell culture supernatant\u003cfont\u003e:\u003c\/font\u003e 91%\t\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eAssay time\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e60 minutes\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies reactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003ci\u003e\u003cspan style=\"font-style:italic;font-size:10.5000pt\"\u003eE. coli\u003c\/span\u003e\u003c\/i\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eE. coli host cell proteins (HCP) constitute a diverse and functionally intricate group of macromolecules within the Escherichia coli cell. The host proteins of E. coli play crucial roles in various cellular processes. These proteins are involved in fundamental metabolic pathways such as glycolysis, the tricarboxylic acid cycle, and amino acid biosynthesis, which are essential for the survival and growth of the bacterium. Recombinant expression in E. coli is a simple and cost-effective method for producing certain proteins. These proteins, often used as therapeutic agents, must be highly purified. The manufacturing process may introduce contamination by HCPs from E. coli, which can reduce efficacy and cause adverse reactions. Therefore, minimizing HCP impurities is crucial. This simple, sensitive, and fast OneStep ELISA is a powerful method for optimizing purification processes, process control, routine quality control, and product release testing.\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"1x96T","offer_id":41677817413707,"sku":"S0C3031-1x96T","price":1200.0,"currency_code":"USD","in_stock":true},{"title":"1x48T","offer_id":43133006118987,"sku":"S0C3031-1x48T","price":300.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/53b6c1f0f4de41b4aa8d354ac9c99c6b.jpg?v=1788433219"},{"product_id":"celltiter-turbo-2-0-luminescentcell-viability-assay-abs50189","title":"CTG-L 2D Luminescent Cell Viability Assay Kit 2.0","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eExperimental procedure:\u003c\/strong\u003e\u003cbr\u003e1. Cell preparation:\u003cbr\u003e(1) Plate 96-well or 384-well cell culture plates with appropriate density of cells to be tested. A whiteboard is recommended.\u003cbr\u003e(2) If the experiment is to determine the effect of the compound on cells, add the appropriate concentration of the compound to be detected to the cell plate well. The concentration of organic solvent in the culture medium is kept below 2%. Continue to cultivate for a suitable time according to the experimental needs.\u003cbr\u003e2. Cell viability detection:\u003cbr\u003e(1) Remove CTG-L\u003csup\u003eTM\u003c\/sup\u003e2D cell viability detection reagent 2.0 [Note 1], equilibrate to room temperature (22 ℃-25 ℃) [Note 2], shake gently and mix well.\u003cbr\u003e(2) Take out the cell culture plate to be tested and equilibrate to room temperature (22 ℃-25 ℃).\u003cbr\u003e(3) Add 50μL of cell viability detection reagent 2.0 to 100μL of 96-well plate cells, or 10μL of reagent to 20μL of 384-well plate cells [Note 3].\u003cbr\u003e(4) Shake the experimental plate for 2 minutes to fully lyse the cells, and place it in a dark place to equilibrate the reaction for 10 minutes.\u003cbr\u003e(5) Read the fluorescence signal on the fluorescence plate reader [Note 4].\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eCTG 2.0 cell viability test kit; ATP cell viability detection kit; 2D Cell Viability Detection Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe ready-to-use cell viability detection kit 2.0 is used to quantitatively detect the ATP content in cells, and the ATP content is directly proportional to the number of viable cells. The reagent can be stored at 4 ℃ for two months after dissolution for the first time, and the reduction of detection signal intensity is \u0026lt; 15%, and there is no loss of function. The reagent has the characteristics of high signal-to-noise ratio, good repeatability and good stability. The ready-to-use formulation reduces the experimental steps of first lysis and then detection, thereby further reducing errors caused by frequent sample loading, and its stable glow signal makes this product especially suitable for high-throughput cell proliferation detection and compound screening.\u003cbr\u003e\u003cstrong\u003eProduct composition:\u003c\/strong\u003e\u003cbr\u003eLuciferase, luciferin and buffer are mixed and filled in 100mL or 500mL brown bottles with the following strengths:\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 72.2222%; height: 88px;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 14.5619%; height: 22px; text-align: center;\"\u003eSpecifications\u003c\/td\u003e\n\u003ctd style=\"width: 25.4341%; height: 22px; text-align: center;\"\u003e96-well plate number of detectable wells\u003c\/td\u003e\n\u003ctd style=\"width: 24.8852%; height: 22px; text-align: center;\"\u003eNumber of detectable 384-well plate wells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 14.5619%; height: 22px; text-align: center;\"\u003e100mL\u003c\/td\u003e\n\u003ctd style=\"width: 25.4341%; height: 22px; text-align: center;\"\u003e2000\u003c\/td\u003e\n\u003ctd style=\"width: 24.8852%; height: 22px; text-align: center;\"\u003e10000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 14.5619%; height: 22px; text-align: center;\"\u003e500mL\u003c\/td\u003e\n\u003ctd style=\"width: 25.4341%; height: 22px; text-align: center;\"\u003e10000\u003c\/td\u003e\n\u003ctd style=\"width: 24.8852%; height: 22px; text-align: center;\"\u003e50000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\u003cdiv\u003e \u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. After the first use, the cell viability detection reagent 2.0 can be packaged and stored at-20 °C and below, protected from light. The reagent was repeatedly freezed and thawed 5 times with a signal intensity reduction of \u0026lt; 10% and no loss of function. The reagent was left at room temperature (22 °C) for 10 days or at 4 °C for two months after dissolution for the first time, and the signal intensity was reduced by \u0026lt; 15% without loss of function.\u003cbr\u003e2. The luciferase reaction in cell viability detection reagent 2.0 is sensitive to temperature changes. Reagents and experimental plates need to be equilibrated to room temperature (22 ℃-25 ℃), and the temperature during the test is kept constant (± 1 ℃)\u003cbr\u003e3. Without strict verification, it is not recommended to change the dosage of reaction reagents at will. The volume ratio of cell culture medium and detection reagent should be 2: 1.\u003cbr\u003e4. Cell viability detection reagent 2.0 has different signal attenuation rates for different cell types, and the signal half-life is between 1.5 hr-4hr. It is recommended that the plate reading time should not exceed 2hr.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eDry Ice Transport. Store at-20 ℃ and below, protected from light, shelf life is 12 months.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100mL","offer_id":41683114131531,"sku":null,"price":241.0,"currency_code":"USD","in_stock":true},{"title":"500mL","offer_id":41683114164299,"sku":null,"price":992.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_8b3dae12-460f-49c6-bca5-e144539568b4.png?v=1770720282"},{"product_id":"2d-fluorescent-cell-viability-assay-kit","title":"CTF-L 2D Fluorescent Cell Viability Assay Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Cell preparation:\u003cbr\u003e(1) Plate 96-well or 384-well cell culture plates with appropriate density of cells to be tested. A whiteboard is recommended.\u003cbr\u003e(2) Prepare the compound to be tested. The appropriate concentration of the compound to be tested is added to the cell plate wells. The concentration of organic solvent in the culture solution is kept below 1-2%. A control group without compound was set up. Continue to cultivate the appropriate time according to the project needs.\u003cbr\u003e2. Cell viability detection:\u003cbr\u003e(1) Remove CTF-L\u003csup\u003eTM\u003c\/sup\u003eCell viability test reagent, equilibrated at room temperature for 20 minutes. Gently shake and mix well.\u003cbr\u003e(2) Prepare CTF reaction solution: The CTF substrate is 100x, use CTF buffer to prepare 1x reaction solution, mix thoroughly, and mix with the same volume of cells to be detected.\u003cbr\u003e(4) Add an equal volume of CTF reaction solution to the wells of the culture plate, shake gently for 20 seconds and mix well, and react at 37 °C in the dark for 60-90 minutes.\u003cbr\u003e(5) Read the fluorescence signal on a plate reader, with an excitation wavelength of 380nm and an emission wavelength of 510nm.\u003cbr\u003e(6) Continue downstream multiplex analysis as needed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThere are many methods to detect cell viability, such as detecting dye exclusion, detecting ATP production, detecting enzyme activity on substrate price reduction, etc. Luciferase assay is widely used and is based on the ability of living cells to produce ATP. Because it is cell detection, sometimes internal reference control is required, which leads to the need for multiplex analysis, that is, the same analysis method is detected at the same time, without interfering with each other, and the detection mechanism is different, so that relevant comparisons can be made to exclude data differences other than experimental factors. Foreign CellTiter Fluor (CTF) products are designed based on this principle. The Company has independently developed and launched a similar Fluorescent Cell Viability Assay Kit (Fluorescent Cell Viability Assay Kit), which has no significant difference in performance from foreign brand products. This product is based on the degradation of polypeptide products by cellular proteases to produce fluorescent signals. When cell membrane integrity is impaired, the protease loses its activity to degrade the substrate. CTF-L\u003csup\u003eTM\u003c\/sup\u003eDifferent from the detection principle of luciferase kit, the detection method is also different. Its detection sensitivity is inferior to that of luciferase method, but it can detect slight cell damage at an earlier stage. The two tests have good compatibility, and multiplex detection can be performed.\u003cbr\u003e\u003cstrong\u003eProduct Features:\u003c\/strong\u003e\u003cbr\u003e1. Performance\u003cbr\u003eThe detection signal, Window, sensitivity and specificity are similar to those of similar products of foreign brands.\u003cbr\u003e2. Compatibility\u003cbr\u003eIt is compatible with commonly used analytical reagents such as CTG and does not form mutual influence.\u003cbr\u003e3. Features\u003cbr\u003eMore sensitive to early membrane damage of cells.\u003cbr\u003e4. Convenience\u003cbr\u003e\"Adding-mixing-incubating-detecting\", the whole experimental steps are simple and easy to operate.\u003cbr\u003e\u003cstrong\u003eProduct composition:\u003c\/strong\u003e\u003cbr\u003eProtease substrate, buffer mixed and filled in 10 mL or 100 mL brown bottles and 2 mL tubes with the following strengths:\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 92.4904%;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 11.7756%; text-align: center;\"\u003eSpecifications\u003c\/td\u003e\n\u003ctd style=\"width: 14.5355%; text-align: center;\"\u003eCTF substrate\u003c\/td\u003e\n\u003ctd style=\"width: 14.6899%; text-align: center;\"\u003eCTF Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 26.5979%; text-align: center;\"\u003e96-well plate number of detectable wells\u003c\/td\u003e\n\u003ctd style=\"width: 26.6683%; text-align: center;\"\u003eNumber of detectable 384-well plate wells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 11.7756%; text-align: center;\"\u003e10mL\u003c\/td\u003e\n\u003ctd style=\"width: 14.5355%; text-align: center;\"\u003e100uL\u003c\/td\u003e\n\u003ctd style=\"width: 14.6899%; text-align: center;\"\u003e10mL\u003c\/td\u003e\n\u003ctd style=\"width: 26.5979%; text-align: center;\"\u003e200 wells\u003c\/td\u003e\n\u003ctd style=\"width: 26.6683%; text-align: center;\"\u003e1000 wells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 11.7756%; text-align: center;\"\u003e2 × 50 mL\u003c\/td\u003e\n\u003ctd style=\"width: 14.5355%; text-align: center;\"\u003e2 × 500 uL\u003c\/td\u003e\n\u003ctd style=\"width: 14.6899%; text-align: center;\"\u003e2 × 50 mL\u003c\/td\u003e\n\u003ctd style=\"width: 26.5979%; text-align: center;\"\u003e2000 wells\u003c\/td\u003e\n\u003ctd style=\"width: 26.6683%; text-align: center;\"\u003e10000 wells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Reagent dosage: It is not recommended to change the dosage of reaction reagents at will unless strictly verified. If a 96-well reaction plate is used, it is generally recommended to add an equal volume of CTF reaction solution to 50uL culture medium, and then add an equal volume of multiplex detection reaction solution (such as CTG reaction solution) as the CTF reaction solution for subsequent detection. Other reaction plates were adjusted for corresponding volumes. It is recommended that the substrate be divided and the reaction solution is freshly prepared from the substrate stock each time.\u003cbr\u003e2. If necessary, adjust the actual remaining culture fluid volume in the reaction wells of the culture plate to ensure that the total multiplex analysis volume does not exceed 200uL (96-well plate) or 40 uL (384-well culture plate).\u003cbr\u003e3. Plate reading time: After adding reagents and incubating at 37 °C for 30 minutes, plate reading can be performed, but its detection sensitivity is lower than that of incubating at 37 °C for 60 minutes or 90 minutes.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore at-20 ℃ and below, protected from light, shelf life is 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"10mL","offer_id":41683114197067,"sku":null,"price":95.0,"currency_code":"USD","in_stock":true},{"title":"50mL×2","offer_id":41683114229835,"sku":null,"price":635.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_4565b020-21cc-4a77-bcf4-3719e553bdea.png?v=1770720282"},{"product_id":"tumor-antigen-specific-t-cell-content-detection-kit","title":"B16F10 melanoma mouse tumor antigen-specific T cell content detection stimulation kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eMethods of use and testing procedures:\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003e1. Add the cells to be detected and the corresponding culture medium to each well of the cell culture dish or cell culture plate, and the cell concentration is 0.8 × 10\u003csup\u003e6\u003c\/sup\u003e-5×10\u003csup\u003e6\u003c\/sup\u003eCells\/mL. Considering the cell loss and the accuracy of flow cytometry detection, it is recommended to use at least 1 million cells. It is recommended to use 12-well or 24-well plate culture, and use at least 1-2mL of culture medium.\u003c\/p\u003e\n\u003cp\u003e2. Add nanoparticles resuspended with PBS or corresponding medium thereto. (The final concentration of nanoparticles used in this test kit is 0.5 mg\/mL-1. 0 mg\/mL)\u003c\/p\u003e\n\u003cp\u003e3. The nanoparticles were incubated with the cells to be detected for 32-48 hours.\u003c\/p\u003e\n\u003cp\u003e4. Add Brefeldin A Solution (BFA) (1000 × diluted into cell culture medium) to the culture medium and incubate for a total of 4 h.\u003c\/p\u003e\n\u003cp\u003e5. Use a pipette to pipette the suspended cells in the collection medium. After collecting the cells, centrifuge the sample cells at 400-500g for 5min. Discard the supernatant and collect the cells.\u003c\/p\u003e\n\u003cp\u003e6. After collecting the cells, resuspend the sample cells in 1 mL PBS.\u003c\/p\u003e\n\u003cp\u003e7. In addition to the blank control, add 2μL of live and dead cell dye to each sample to label live cells and dead cells, mix evenly, and incubate on ice for 15-30 minutes in the dark.\u003c\/p\u003e\n\u003cp\u003e8. After incubation, centrifuge at 400-500g for 5 min, and resuspend the cells with 100μL FACS buffer.\u003c\/p\u003e\n\u003cp\u003e9. Except for the blank control. Add 2 μL Fc Block to each sample to block the Fc receptor on the surface of immune cells, mix well and incubate on ice for 5 min.\u003c\/p\u003e\n\u003cp\u003e10. After incubation, add corresponding membrane-labeled antibodies CD3 antibody, CD4 antibody, and CD8 antibody labeled with different fluorescent dyes to each tube, and incubate on ice for 15-30min.\u003c\/p\u003e\n\u003cp\u003e11. Add 500μL of 4% paraformaldehyde directly to fix the cells and incubate on ice for 40-60min.\u003c\/p\u003e\n\u003cp\u003e12. Centrifuge at 400-500g for 5min, discard the supernatant, then add 100μL of membrane breaking agent to resuspend the cells, and incubate on ice for 30min.\u003c\/p\u003e\n\u003cp\u003e13. After incubation, add the corresponding fluorescent dye-labeled IFN-γ antibody to each tube and incubate on ice for 30 minutes.\u003c\/p\u003e\n\u003cp\u003e14. Centrifuge at 400-500g for 5min, discard the supernatant, resuspend the cells with 200μL FACS buffer or fix the cells with 500μL 4% paraformaldehyde, incubate on ice for 40-60min before detection.\u003c\/p\u003e\n\u003cp\u003e15. Use flow cytometry to detect the proportion of cells that are positive for the labeled antibody. (Note: It is necessary to pass through 400 mesh filter cloth before testing on the machine)\u003c\/p\u003e\n\u003cp\u003e16. Use flow cytometry analysis software to analyze the proportion of CD3 + IFN-γ +, CD3+CD4 + IFN-γ + and CD3+CD8 + IFN-γ + T cells in living cells to the corresponding cells, that is, the proportion and content of tumor antigen-specific T cells (the more cells, the more accurate the detection. It is recommended that the number of living cells collected during detection is more than 100,000, and the number of living cells collected is preferably more than 200,000).\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003eTumor antigen-specific T cells are the main force in killing cancer cells. Due to the high heterogeneity of cancer cells and tumor antigens, cancer cells do not have one or more dominant antigens, so there are few dominant antigen-specific T cells. However, the traditional use of several polypeptide antigens or MHC tetramer technology to detect tumor antigen-specific T cells can only detect antigen-specific T cells against several known specific antigens.\u003c\/p\u003e\n\u003cp\u003eThis product detects the whole cell antigen of cancer cells loaded with nanoparticles. The types of antigens are comprehensive, diverse and broad-spectrum, and can comprehensively and accurately detect tumor antigen-specific T cells in different tissues. It has the advantages of high specificity, high accuracy, comprehensive types of tumor antigen-specific T cells detected, and the like.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTest samples:\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003e1. The content of tumor antigen-specific T cells in peripheral blood immune cells of mice;\u003c\/p\u003e\n\u003cp\u003e2. The content of tumor antigen-specific T cells in mouse spleen cells;\u003c\/p\u003e\n\u003cp\u003e3. The content of tumor antigen-specific T cells in mouse lymph node cells;\u003c\/p\u003e\n\u003cp\u003e4. The content of tumor antigen-specific T cells in mouse tumor tissue cells;\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"border-collapse: collapse; width: 35.5212%; height: 131px;\" border=\"1\" width=\"310.52pt\" cellspacing=\"0\" cellpadding=\"0\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et2\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" width=\"139\" height=\"33\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 32.1992%; text-align: center;\" width=\"138\"\u003e\u003cstrong\u003e20T\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 31.982%; text-align: center;\" width=\"137\"\u003e\u003cstrong\u003e100T\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et3\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" height=\"33\"\u003eNanoparticles\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 32.1992%; text-align: center;\"\u003e20mg\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 31.982%; text-align: center;\"\u003e100mg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et4\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" height=\"33\"\u003ePBS\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 32.1992%; text-align: center;\"\u003e2ml\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 31.982%; text-align: center;\"\u003e10ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1. This product is only for in vitro testing, and cannot be edible or injected; Please keep away from the reach of children.\u003c\/p\u003e\n\u003cp\u003e2. Prepare and use it now. Once the nanoparticles are resuspended with culture medium or PBS, immediately add them to the cells to be tested and mix.\u003c\/p\u003e\n\u003cp\u003e3. During use, the fluorescent probes of different antibodies used can be freely matched as needed.\u003c\/p\u003e\n\u003cp\u003e4. Please place the fixed cells at 4 °C and complete the test within one week.\u003c\/p\u003e\n\u003cp\u003e5. This product is only suitable for testing mouse samples.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFreeze-dry storage at-20 ℃ ~-80 ℃, valid for 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"20T","offer_id":41683114983499,"sku":null,"price":171.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":41683115016267,"sku":null,"price":679.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_3590b9b6-4561-458d-9311-09d5369171ad.png?v=1770720280"},{"product_id":"tumor-antigen-specific-t-cell-content-detection-kit-abs57002","title":"B16F10-OVA melanoma mouse tumor antigen-specific T cell content detection stimulation kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eMethods of use and testing procedures:\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003e1. Add the cells to be detected and the corresponding culture medium to each well of the cell culture dish or cell culture plate, and the cell concentration is 0.8 × 10\u003csup\u003e6\u003c\/sup\u003e-5×10\u003csup\u003e6\u003c\/sup\u003eCells\/mL. Considering the cell loss and the accuracy of flow cytometry detection, it is recommended to use at least 1 million cells. It is recommended to use 12-well or 24-well plate culture, and use at least 1-2mL of culture medium.\u003c\/p\u003e\n\u003cp\u003e2. Add nanoparticles resuspended with PBS or corresponding medium thereto. (The final concentration of nanoparticles used in this test kit is 0.5 mg\/mL-1. 0 mg\/mL)\u003c\/p\u003e\n\u003cp\u003e3. The nanoparticles were incubated with the cells to be detected for 32-48 hours.\u003c\/p\u003e\n\u003cp\u003e4. Add Brefeldin A Solution (BFA) (1000 × diluted into cell culture medium) to the culture medium and incubate for a total of 4 h.\u003c\/p\u003e\n\u003cp\u003e5. Use a pipette to pipette the suspended cells in the collection medium. After collecting the cells, centrifuge the sample cells at 400-500g for 5min. Discard the supernatant and collect the cells.\u003c\/p\u003e\n\u003cp\u003e6. After collecting the cells, resuspend the sample cells in 1 mL PBS.\u003c\/p\u003e\n\u003cp\u003e7. In addition to the blank control, add 2μL of live and dead cell dye to each sample to label live cells and dead cells, mix evenly, and incubate on ice for 15-30 minutes in the dark.\u003c\/p\u003e\n\u003cp\u003e8. After incubation, centrifuge at 400-500g for 5 min, and resuspend the cells with 100μL FACS buffer.\u003c\/p\u003e\n\u003cp\u003e9. Except for the blank control. Add 2 μL Fc Block to each sample to block the Fc receptor on the surface of immune cells, mix well and incubate on ice for 5 min.\u003c\/p\u003e\n\u003cp\u003e10. After incubation, add corresponding membrane-labeled antibodies CD3 antibody, CD4 antibody, and CD8 antibody labeled with different fluorescent dyes to each tube, and incubate on ice for 15-30min.\u003c\/p\u003e\n\u003cp\u003e11. Add 500μL of 4% paraformaldehyde directly to fix the cells and incubate on ice for 40-60min.\u003c\/p\u003e\n\u003cp\u003e12. Centrifuge at 400-500g for 5min, discard the supernatant, then add 100μL of membrane breaking agent to resuspend the cells, and incubate on ice for 30min.\u003c\/p\u003e\n\u003cp\u003e13. After incubation, add the corresponding fluorescent dye-labeled IFN-γ antibody to each tube and incubate on ice for 30 minutes.\u003c\/p\u003e\n\u003cp\u003e14. Centrifuge at 400-500g for 5min, discard the supernatant, resuspend the cells with 200μL FACS buffer or fix the cells with 500μL 4% paraformaldehyde, incubate on ice for 40-60min before detection.\u003c\/p\u003e\n\u003cp\u003e15. Use flow cytometry to detect the proportion of cells that are positive for the labeled antibody. (Note: It is necessary to pass through 400 mesh filter cloth before testing on the machine)\u003c\/p\u003e\n\u003cp\u003e16. Use flow cytometry analysis software to analyze the proportion of CD3 + IFN-γ +, CD3+CD4 + IFN-γ + and CD3+CD8 + IFN-γ + T cells in living cells to the corresponding cells, that is, the proportion and content of tumor antigen-specific T cells (the more cells, the more accurate the detection. It is recommended that the number of living cells collected during detection is more than 100,000, and the number of living cells collected is preferably more than 200,000).\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003eTumor antigen-specific T cells are the main force in killing cancer cells. Due to the high heterogeneity of cancer cells and tumor antigens, cancer cells do not have one or more dominant antigens, so there are few dominant antigen-specific T cells. However, the traditional use of several polypeptide antigens or MHC tetramer technology to detect tumor antigen-specific T cells can only detect antigen-specific T cells against several known specific antigens.\u003c\/p\u003e\n\u003cp\u003eThis product detects the whole cell antigen of cancer cells loaded with nanoparticles. The types of antigens are comprehensive, diverse and broad-spectrum, and can comprehensively and accurately detect tumor antigen-specific T cells in different tissues. It has the advantages of high specificity, high accuracy, comprehensive types of tumor antigen-specific T cells detected, and the like.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTest samples:\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003e1. The content of tumor antigen-specific T cells in peripheral blood immune cells of mice;\u003c\/p\u003e\n\u003cp\u003e2. The content of tumor antigen-specific T cells in mouse spleen cells;\u003c\/p\u003e\n\u003cp\u003e3. The content of tumor antigen-specific T cells in mouse lymph node cells;\u003c\/p\u003e\n\u003cp\u003e4. The content of tumor antigen-specific T cells in mouse tumor tissue cells;\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"border-collapse: collapse; width: 35.5212%; height: 131px;\" border=\"1\" width=\"310.52pt\" cellspacing=\"0\" cellpadding=\"0\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et2\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" width=\"139\" height=\"33\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 32.1992%; text-align: center;\" width=\"138\"\u003e\u003cstrong\u003e20T\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 31.982%; text-align: center;\" width=\"137\"\u003e\u003cstrong\u003e100T\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et3\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" height=\"33\"\u003eNanoparticles\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 32.1992%; text-align: center;\"\u003e20mg\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 31.982%; text-align: center;\"\u003e100mg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et4\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" height=\"33\"\u003ePBS\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 32.1992%; text-align: center;\"\u003e2ml\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 31.982%; text-align: center;\"\u003e10ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1. This product is only for in vitro testing, and cannot be edible or injected; Please keep away from the reach of children.\u003c\/p\u003e\n\u003cp\u003e2. Prepare and use it now. Once the nanoparticles are resuspended with culture medium or PBS, immediately add them to the cells to be tested and mix.\u003c\/p\u003e\n\u003cp\u003e3. During use, the fluorescent probes of different antibodies used can be freely matched as needed.\u003c\/p\u003e\n\u003cp\u003e4. Please place the fixed cells at 4 °C and complete the test within one week.\u003c\/p\u003e\n\u003cp\u003e5. This product is only suitable for testing mouse samples.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFreeze-dry storage at-20 ℃ ~-80 ℃, valid for 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"20T","offer_id":41683115180107,"sku":null,"price":171.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":41683115212875,"sku":null,"price":679.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_c660edb9-3b56-4719-ac1f-ce33da4de29f.png?v=1770720279"},{"product_id":"tumor-antigen-specific-t-cell-content-detection-kit-abs57003","title":"B16F10 - Fluc melanoma tumor antigen specific T cells in mice content test stimulus kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eMethods of use and testing procedures:\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003e1. Add the cells to be detected and the corresponding culture medium to each well of the cell culture dish or cell culture plate, and the cell concentration is 0.8 × 10\u003csup\u003e6\u003c\/sup\u003e-5×10\u003csup\u003e6\u003c\/sup\u003eCells\/mL. Considering the cell loss and the accuracy of flow cytometry detection, it is recommended to use at least 1 million cells. It is recommended to use 12-well or 24-well plate culture, and use at least 1-2mL of culture medium.\u003c\/p\u003e\n\u003cp\u003e2. Add nanoparticles after resuspension with PBS or corresponding medium thereto. (The final concentration of nanoparticles used in this test kit is 0.5 mg\/mL-1. 0 mg\/mL)\u003c\/p\u003e\n\u003cp\u003e3. The nanoparticles were incubated with the cells to be detected for 32-48 hours.\u003c\/p\u003e\n\u003cp\u003e4. Add Brefeldin A Solution (BFA) (1000 × diluted into cell culture medium) to the culture medium and incubate for a total of 4 h.\u003c\/p\u003e\n\u003cp\u003e5. Use a pipette to pipette the suspended cells in the collection medium. After collecting the cells, centrifuge the sample cells at 400-500g for 5min. Discard the supernatant and collect the cells.\u003c\/p\u003e\n\u003cp\u003e6. After collecting the cells, resuspend the sample cells in 1 mL PBS.\u003c\/p\u003e\n\u003cp\u003e7. In addition to the blank control, add 2μL of live and dead cell dye to each sample to label live cells and dead cells, mix evenly, and incubate on ice for 15-30 minutes in the dark.\u003c\/p\u003e\n\u003cp\u003e8. After incubation, centrifuge at 400-500g for 5 min, and resuspend the cells with 100μL FACS buffer.\u003c\/p\u003e\n\u003cp\u003e9. Except for the blank control. Add 2 μL Fc Block to each sample to block the Fc receptor on the surface of immune cells, mix well and incubate on ice for 5 min.\u003c\/p\u003e\n\u003cp\u003e10. After incubation, add corresponding membrane-labeled antibodies CD3 antibody, CD4 antibody, and CD8 antibody labeled with different fluorescent dyes to each tube, and incubate on ice for 15-30min.\u003c\/p\u003e\n\u003cp\u003e11. Add 500μL of 4% paraformaldehyde directly to fix the cells and incubate on ice for 40-60min.\u003c\/p\u003e\n\u003cp\u003e12. Centrifuge at 400-500g for 5min, discard the supernatant, then add 100μL of membrane breaking agent to resuspend the cells, and incubate on ice for 30min.\u003c\/p\u003e\n\u003cp\u003e13. After incubation, add the corresponding fluorescent dye-labeled IFN-γ antibody to each tube and incubate on ice for 30 minutes.\u003c\/p\u003e\n\u003cp\u003e14. Centrifuge at 400-500g for 5min, discard the supernatant, resuspend the cells with 200μL FACS buffer or fix the cells with 500μL 4% paraformaldehyde, incubate on ice for 40-60min before detection.\u003c\/p\u003e\n\u003cp\u003e15. Use flow cytometry to detect the proportion of cells that are positive for the labeled antibody. (Note: It is necessary to pass through 400 mesh filter cloth before testing on the machine)\u003c\/p\u003e\n\u003cp\u003e16. Use flow cytometry analysis software to analyze the proportion of CD3 + IFN-γ +, CD3+CD4 + IFN-γ + and CD3+CD8 + IFN-γ + T cells in living cells to the corresponding cells, that is, the proportion and content of tumor antigen-specific T cells (the more cells, the more accurate the detection. It is recommended that the number of living cells collected during detection is more than 100,000, and the number of living cells collected is preferably more than 200,000).\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003eTumor antigen-specific T cells are the main force in killing cancer cells. Due to the high heterogeneity of cancer cells and tumor antigens, cancer cells do not have one or more dominant antigens, so there are few dominant antigen-specific T cells. However, the traditional use of several polypeptide antigens or MHC tetramer technology to detect tumor antigen-specific T cells can only detect antigen-specific T cells against several known specific antigens.\u003c\/p\u003e\n\u003cp\u003eThis product detects the whole cell antigen of cancer cells loaded with nanoparticles. The types of antigens are comprehensive, diverse and broad-spectrum, and can comprehensively and accurately detect tumor antigen-specific T cells in different tissues. It has the advantages of high specificity, high accuracy, comprehensive types of tumor antigen-specific T cells detected, and the like.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTest samples:\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003e1. The content of tumor antigen-specific T cells in peripheral blood immune cells of mice;\u003c\/p\u003e\n\u003cp\u003e2. The content of tumor antigen-specific T cells in mouse spleen cells;\u003c\/p\u003e\n\u003cp\u003e3. The content of tumor antigen-specific T cells in mouse lymph node cells;\u003c\/p\u003e\n\u003cp\u003e4. The content of tumor antigen-specific T cells in mouse tumor tissue cells;\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"border-collapse: collapse; width: 35.5212%; height: 131px;\" border=\"1\" width=\"310.52pt\" cellspacing=\"0\" cellpadding=\"0\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et2\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" width=\"139\" height=\"33\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 32.1992%; text-align: center;\" width=\"138\"\u003e\u003cstrong\u003e20T\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 31.982%; text-align: center;\" width=\"137\"\u003e\u003cstrong\u003e100T\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et3\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" height=\"33\"\u003eNanoparticles\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 32.1992%; text-align: center;\"\u003e20mg\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 31.982%; text-align: center;\"\u003e100mg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et4\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" height=\"33\"\u003ePBS\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 32.1992%; text-align: center;\"\u003e2ml\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 31.982%; text-align: center;\"\u003e10ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1. This product is only for in vitro testing, and cannot be edible or injected; Please keep away from the reach of children.\u003c\/p\u003e\n\u003cp\u003e2. Prepare and use it now. Once the nanoparticles are resuspended with culture medium or PBS, immediately add them to the cells to be tested and mix.\u003c\/p\u003e\n\u003cp\u003e3. During use, the fluorescent probes of different antibodies used can be freely matched as needed.\u003c\/p\u003e\n\u003cp\u003e4. Please place the fixed cells at 4 °C and complete the test within one week.\u003c\/p\u003e\n\u003cp\u003e5. This product is only suitable for testing mouse samples.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFreeze-dry storage at-20 ℃ ~-80 ℃, valid for 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"20T","offer_id":41683115278411,"sku":null,"price":171.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":41683115311179,"sku":null,"price":679.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_9d201e1a-2d0a-4752-b56b-99fe806c153d.png?v=1770720278"},{"product_id":"tumor-antigen-specific-t-cell-content-detection-kit-abs57004","title":"LLC lung cancer mouse tumor antigen-specific T cell content detection stimulation kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eMethods of use and testing procedures:\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003e1. Add the cells to be detected and the corresponding culture medium to each well of the cell culture dish or cell culture plate, and the cell concentration is 0.8 × 10\u003csup\u003e6\u003c\/sup\u003e-5×10\u003csup\u003e6\u003c\/sup\u003eCells\/mL. Considering the cell loss and the accuracy of flow cytometry detection, it is recommended to use at least 1 million cells. It is recommended to use 12-well or 24-well plate culture, and use at least 1-2mL of culture medium.\u003c\/p\u003e\n\u003cp\u003e2. Add nanoparticles resuspended with PBS or corresponding medium thereto. (The final concentration of nanoparticles used in this test kit is 0.5 mg\/mL-1. 0 mg\/mL)\u003c\/p\u003e\n\u003cp\u003e3. The nanoparticles were incubated with the cells to be detected for 32-48 hours.\u003c\/p\u003e\n\u003cp\u003e4. Add Brefeldin A Solution (BFA) (1000 × diluted into cell culture medium) to the culture medium and incubate for a total of 4 h.\u003c\/p\u003e\n\u003cp\u003e5. Use a pipette to pipette the suspended cells in the collection medium. After collecting the cells, centrifuge the sample cells at 400-500g for 5min. Discard the supernatant and collect the cells.\u003c\/p\u003e\n\u003cp\u003e6. After collecting the cells, resuspend the sample cells in 1 mL PBS.\u003c\/p\u003e\n\u003cp\u003e7. In addition to the blank control, add 2μL of live and dead cell dye to each sample to label live cells and dead cells, mix evenly, and incubate on ice for 15-30 minutes in the dark.\u003c\/p\u003e\n\u003cp\u003e8. After incubation, centrifuge at 400-500g for 5 min, and resuspend the cells with 100μL FACS buffer.\u003c\/p\u003e\n\u003cp\u003e9. Except for the blank control. Add 2 μL Fc Block to each sample to block the Fc receptor on the surface of immune cells, mix well and incubate on ice for 5 min.\u003c\/p\u003e\n\u003cp\u003e10. After incubation, add corresponding membrane-labeled antibodies CD3 antibody, CD4 antibody, and CD8 antibody labeled with different fluorescent dyes to each tube, and incubate on ice for 15-30min.\u003c\/p\u003e\n\u003cp\u003e11. Add 500μL of 4% paraformaldehyde directly to fix the cells and incubate on ice for 40-60min.\u003c\/p\u003e\n\u003cp\u003e12. Centrifuge at 400-500g for 5min, discard the supernatant, then add 100μL of membrane breaking agent to resuspend the cells, and incubate on ice for 30min.\u003c\/p\u003e\n\u003cp\u003e13. After incubation, add the corresponding fluorescent dye-labeled IFN-γ antibody to each tube and incubate on ice for 30 minutes.\u003c\/p\u003e\n\u003cp\u003e14. Centrifuge at 400-500g for 5min, discard the supernatant, resuspend the cells with 200μL FACS buffer or fix the cells with 500μL 4% paraformaldehyde, incubate on ice for 40-60min before detection.\u003c\/p\u003e\n\u003cp\u003e15. Use flow cytometry to detect the proportion of cells that are positive for the labeled antibody. (Note: It is necessary to pass through 400 mesh filter cloth before testing on the machine)\u003c\/p\u003e\n\u003cp\u003e16. Use flow cytometry analysis software to analyze the proportion of CD3 + IFN-γ +, CD3+CD4 + IFN-γ + and CD3+CD8 + IFN-γ + T cells in living cells to the corresponding cells, that is, the proportion and content of tumor antigen-specific T cells (the more cells, the more accurate the detection. It is recommended that the number of living cells collected during detection is more than 100,000, and the number of living cells collected is preferably more than 200,000).\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003eTumor antigen-specific T cells are the main force in killing cancer cells. Due to the high heterogeneity of cancer cells and tumor antigens, cancer cells do not have one or more dominant antigens, so there are few dominant antigen-specific T cells. However, the traditional use of several polypeptide antigens or MHC tetramer technology to detect tumor antigen-specific T cells can only detect antigen-specific T cells against several known specific antigens.\u003c\/p\u003e\n\u003cp\u003eThis product detects the whole cell antigens of cancer cells loaded with nanoparticles. The types of antigens are comprehensive, diverse and broad-spectrum, and can comprehensively and accurately detect tumor antigen-specific T cells in different tissues. It has the advantages of high specificity, high accuracy, comprehensive types of tumor antigen-specific T cells detected, and the like.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTest samples:\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003e1. The content of tumor antigen-specific T cells in peripheral blood immune cells of mice;\u003c\/p\u003e\n\u003cp\u003e2. The content of tumor antigen-specific T cells in mouse spleen cells;\u003c\/p\u003e\n\u003cp\u003e3. The content of tumor antigen-specific T cells in mouse lymph node cells;\u003c\/p\u003e\n\u003cp\u003e4. The content of tumor antigen-specific T cells in mouse tumor tissue cells;\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"border-collapse: collapse; width: 35.5212%; height: 131px;\" border=\"1\" width=\"310.52pt\" cellspacing=\"0\" cellpadding=\"0\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et2\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" width=\"139\" height=\"33\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 32.1992%; text-align: center;\" width=\"138\"\u003e\u003cstrong\u003e20T\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 31.982%; text-align: center;\" width=\"137\"\u003e\u003cstrong\u003e100T\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et3\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" height=\"33\"\u003eNanoparticles\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 32.1992%; text-align: center;\"\u003e20mg\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 31.982%; text-align: center;\"\u003e100mg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et4\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" height=\"33\"\u003ePBS\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 32.1992%; text-align: center;\"\u003e2ml\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 31.982%; text-align: center;\"\u003e10ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1. This product is only for in vitro testing, and cannot be edible or injected; Please keep away from the reach of children.\u003c\/p\u003e\n\u003cp\u003e2. Prepare and use it now. Once the nanoparticles are resuspended with culture medium or PBS, immediately add them to the cells to be tested and mix.\u003c\/p\u003e\n\u003cp\u003e3. During use, the fluorescent probes of different antibodies used can be freely matched as needed.\u003c\/p\u003e\n\u003cp\u003e4. Please place the fixed cells at 4 °C and complete the test within one week.\u003c\/p\u003e\n\u003cp\u003e5. This product is only suitable for testing mouse samples.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFreeze-dry storage at-20 ℃ ~-80 ℃, valid for 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"20T","offer_id":41683115343947,"sku":null,"price":171.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":41683115376715,"sku":null,"price":679.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_4ba3ea04-b40f-473f-b94b-e62923afeb45.png?v=1770720278"},{"product_id":"tumor-antigen-specific-t-cell-content-detection-kit-abs57005","title":"LLC-Fluc lung cancer Mouse tumor antigen-specific T cell content detection stimulation kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eMethods of use and testing procedures:\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003e1. Add the cells to be detected and the corresponding culture medium to each well of the cell culture dish or cell culture plate, and the cell concentration is 0.8 × 10\u003csup\u003e6\u003c\/sup\u003e-5×10\u003csup\u003e6\u003c\/sup\u003eCells\/mL. Considering the cell loss and the accuracy of flow cytometry detection, it is recommended to use at least 1 million cells. It is recommended to use 12-well or 24-well plate culture, and use at least 1-2mL of culture medium.\u003c\/p\u003e\n\u003cp\u003e2. Add nanoparticles resuspended with PBS or corresponding medium thereto. (The final concentration of nanoparticles used in this test kit is 0.5 mg\/mL-1. 0 mg\/mL)\u003c\/p\u003e\n\u003cp\u003e3. The nanoparticles were incubated with the cells to be detected for 32-48 hours.\u003c\/p\u003e\n\u003cp\u003e4. Add Brefeldin A Solution (BFA) (1000 × diluted into cell culture medium) to the culture medium and incubate for a total of 4 h.\u003c\/p\u003e\n\u003cp\u003e5. Use a pipette to pipette the suspended cells in the collection medium. After collecting the cells, centrifuge the sample cells at 400-500g for 5min. Discard the supernatant and collect the cells.\u003c\/p\u003e\n\u003cp\u003e6. After collecting the cells, resuspend the sample cells in 1 mL PBS.\u003c\/p\u003e\n\u003cp\u003e7. In addition to the blank control, add 2μL of live and dead cell dye to each sample to label live cells and dead cells, mix evenly, and incubate on ice for 15-30 minutes in the dark.\u003c\/p\u003e\n\u003cp\u003e8. After incubation, centrifuge at 400-500g for 5 min, and resuspend the cells with 100μL FACS buffer.\u003c\/p\u003e\n\u003cp\u003e9. Except for the blank control. Add 2 μL Fc Block to each sample to block the Fc receptor on the surface of immune cells, mix well and incubate on ice for 5 min.\u003c\/p\u003e\n\u003cp\u003e10. After incubation, add corresponding membrane-labeled antibodies CD3 antibody, CD4 antibody, and CD8 antibody labeled with different fluorescent dyes to each tube, and incubate on ice for 15-30min.\u003c\/p\u003e\n\u003cp\u003e11. Add 500μL of 4% paraformaldehyde directly to fix the cells and incubate on ice for 40-60min.\u003c\/p\u003e\n\u003cp\u003e12. Centrifuge at 400-500g for 5min, discard the supernatant, then add 100μL of membrane breaking agent to resuspend the cells, and incubate on ice for 30min.\u003c\/p\u003e\n\u003cp\u003e13. After incubation, add the corresponding fluorescent dye-labeled IFN-γ antibody to each tube and incubate on ice for 30 minutes.\u003c\/p\u003e\n\u003cp\u003e14. Centrifuge at 400-500g for 5min, discard the supernatant, resuspend the cells with 200μL FACS buffer or fix the cells with 500μL 4% paraformaldehyde, incubate on ice for 40-60min before detection.\u003c\/p\u003e\n\u003cp\u003e15. Use flow cytometry to detect the proportion of cells that are positive for the labeled antibody. (Note: It is necessary to pass through 400 mesh filter cloth before testing on the machine)\u003c\/p\u003e\n\u003cp\u003e16. Use flow cytometry analysis software to analyze the proportion of CD3 + IFN-γ +, CD3+CD4 + IFN-γ + and CD3+CD8 + IFN-γ + T cells in living cells to the corresponding cells, that is, the proportion and content of tumor antigen-specific T cells (the more cells, the more accurate the detection. It is recommended that the number of living cells collected during detection is more than 100,000, and the number of living cells collected is preferably more than 200,000).\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003eTumor antigen-specific T cells are the main force in killing cancer cells. Due to the high heterogeneity of cancer cells and tumor antigens, cancer cells do not have one or more dominant antigens, so there are few dominant antigen-specific T cells. However, the traditional use of several polypeptide antigens or MHC tetramer technology to detect tumor antigen-specific T cells can only detect antigen-specific T cells against several known specific antigens.\u003c\/p\u003e\n\u003cp\u003eThis product detects the whole cell antigen of cancer cells loaded with nanoparticles. The types of antigens are comprehensive, diverse and broad-spectrum, and can comprehensively and accurately detect tumor antigen-specific T cells in different tissues. Has the advantages of high specificity, high accuracy, comprehensive types of tumor antigen-specific T cells detected, and the like.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTest samples:\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003e1. The content of tumor antigen-specific T cells in peripheral blood immune cells of mice;\u003c\/p\u003e\n\u003cp\u003e2. The content of tumor antigen-specific T cells in mouse spleen cells;\u003c\/p\u003e\n\u003cp\u003e3. The content of tumor antigen-specific T cells in mouse lymph node cells;\u003c\/p\u003e\n\u003cp\u003e4. The content of tumor antigen-specific T cells in mouse tumor tissue cells;\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"border-collapse: collapse; width: 35.5212%; height: 131px;\" border=\"1\" width=\"310.52pt\" cellspacing=\"0\" cellpadding=\"0\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et2\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" width=\"139\" height=\"33\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 32.1992%; text-align: center;\" width=\"138\"\u003e\u003cstrong\u003e20T\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 31.982%; text-align: center;\" width=\"137\"\u003e\u003cstrong\u003e100T\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et3\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" height=\"33\"\u003eNanoparticles\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 32.1992%; text-align: center;\"\u003e20mg\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 31.982%; text-align: center;\"\u003e100mg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et4\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" height=\"33\"\u003ePBS\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 32.1992%; text-align: center;\"\u003e2ml\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 31.982%; text-align: center;\"\u003e10ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. This product is only for in vitro testing, and cannot be edible or injected; Please keep away from the reach of children.\u003cbr\u003e2. Prepare and use it now. Once the nanoparticles are resuspended with culture medium or PBS, immediately add them to the cells to be tested and mix.\u003cbr\u003e3. During use, the fluorescent probes of different antibodies used can be freely matched as needed.\u003cbr\u003e4. Please place the fixed cells at 4 °C and complete the test within one week.\u003cbr\u003e5. This product is only suitable for testing mouse samples.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFreeze-dry storage at-20 ℃ ~-80 ℃, valid for 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"20T","offer_id":41683115409483,"sku":null,"price":171.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":41683115442251,"sku":null,"price":679.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_889c09c5-e7f2-4fbb-93a4-f0e0525cb6b2.png?v=1770720278"},{"product_id":"tumor-antigen-specific-t-cell-content-detection-kit-abs57006","title":"LLC - OVA lung tumor antigen specific T cells in mice content test stimulus kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eMethods of use and testing procedures:\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003e1. Add the cells to be detected and the corresponding culture medium to each well of the cell culture dish or cell culture plate, and the cell concentration is 0.8 × 10\u003csup\u003e6\u003c\/sup\u003e-5×10\u003csup\u003e6\u003c\/sup\u003eCells\/mL. Considering the cell loss and the accuracy of flow cytometry detection, it is recommended to use at least 1 million cells. It is recommended to use 12-well or 24-well plate culture, and use at least 1-2mL of culture medium.\u003c\/p\u003e\n\u003cp\u003e2. Add nanoparticles resuspended with PBS or corresponding medium thereto. (The final concentration of nanoparticles used in this test kit is 0.5 mg\/mL-1. 0 mg\/mL)\u003c\/p\u003e\n\u003cp\u003e3. The nanoparticles were incubated with the cells to be detected for 32-48 hours.\u003c\/p\u003e\n\u003cp\u003e4. Add Brefeldin A Solution (BFA) (1000 × diluted into cell culture medium) to the culture medium and incubate for a total of 4 h.\u003c\/p\u003e\n\u003cp\u003e5. Use a pipette to pipette the suspended cells in the collection medium. After collecting the cells, centrifuge the sample cells at 400-500g for 5min. Discard the supernatant and collect the cells.\u003c\/p\u003e\n\u003cp\u003e6. After collecting the cells, resuspend the sample cells in 1 mL PBS.\u003c\/p\u003e\n\u003cp\u003e7. In addition to the blank control, add 2μL of live and dead cell dye to each sample to label live cells and dead cells, mix evenly, and incubate on ice for 15-30 minutes in the dark.\u003c\/p\u003e\n\u003cp\u003e8. After incubation, centrifuge at 400-500g for 5 min, and resuspend the cells with 100μL FACS buffer.\u003c\/p\u003e\n\u003cp\u003e9. Except for the blank control. Add 2 μL Fc Block to each sample to block the Fc receptor on the surface of immune cells, mix well and incubate on ice for 5 min.\u003c\/p\u003e\n\u003cp\u003e10. After incubation, add corresponding membrane-labeled antibodies CD3 antibody, CD4 antibody, and CD8 antibody labeled with different fluorescent dyes to each tube, and incubate on ice for 15-30min.\u003c\/p\u003e\n\u003cp\u003e11. Add 500μL of 4% paraformaldehyde directly to fix the cells and incubate on ice for 40-60min.\u003c\/p\u003e\n\u003cp\u003e12. Centrifuge at 400-500g for 5min, discard the supernatant, then add 100μL of membrane breaking agent to resuspend the cells, and incubate on ice for 30min.\u003c\/p\u003e\n\u003cp\u003e13. After incubation, add the corresponding fluorescent dye-labeled IFN-γ antibody to each tube and incubate on ice for 30 minutes.\u003c\/p\u003e\n\u003cp\u003e14. Centrifuge at 400-500g for 5min, discard the supernatant, resuspend the cells with 200μL FACS buffer or fix the cells with 500μL 4% paraformaldehyde, incubate on ice for 40-60min before detection.\u003c\/p\u003e\n\u003cp\u003e15. Use flow cytometry to detect the proportion of cells that are positive for the labeled antibody. (Note: It is necessary to pass through 400 mesh filter cloth before testing on the machine)\u003c\/p\u003e\n\u003cp\u003e16. Use flow cytometry analysis software to analyze the proportion of CD3 + IFN-γ +, CD3+CD4 + IFN-γ + and CD3+CD8 + IFN-γ + T cells in living cells to the corresponding cells, that is, the proportion and content of tumor antigen-specific T cells (the more cells, the more accurate the detection. It is recommended that the number of living cells collected during detection is more than 100,000, and the number of living cells collected is preferably more than 200,000).\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003eTumor antigen-specific T cells are the main force in killing cancer cells. Due to the high heterogeneity of cancer cells and tumor antigens, cancer cells do not have one or more dominant antigens, so there are few dominant antigen-specific T cells. However, the traditional use of several polypeptide antigens or MHC tetramer technology to detect tumor antigen-specific T cells can only detect antigen-specific T cells against several known specific antigens.\u003c\/p\u003e\n\u003cp\u003eThis product detects the whole cell antigens of cancer cells loaded with nanoparticles. The types of antigens are comprehensive, diverse and broad-spectrum, and can comprehensively and accurately detect tumor antigen-specific T cells in different tissues. Has the advantages of high specificity, high accuracy, comprehensive types of tumor antigen-specific T cells detected, and the like.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTest samples:\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003e1. The content of tumor antigen-specific T cells in peripheral blood immune cells of mice;\u003c\/p\u003e\n\u003cp\u003e2. The content of tumor antigen-specific T cells in mouse spleen cells;\u003c\/p\u003e\n\u003cp\u003e3. The content of tumor antigen-specific T cells in mouse lymph node cells;\u003c\/p\u003e\n\u003cp\u003e4. The content of tumor antigen-specific T cells in mouse tumor tissue cells;\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"border-collapse: collapse; width: 35.5212%; height: 131px;\" border=\"1\" width=\"310.52pt\" cellspacing=\"0\" cellpadding=\"0\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et2\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" width=\"139\" height=\"33\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 32.1992%; text-align: center;\" width=\"138\"\u003e\u003cstrong\u003e20T\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 31.982%; text-align: center;\" width=\"137\"\u003e\u003cstrong\u003e100T\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et3\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" height=\"33\"\u003eNanoparticles\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 32.1992%; text-align: center;\"\u003e20mg\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 31.982%; text-align: center;\"\u003e100mg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et4\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" height=\"33\"\u003ePBS\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 32.1992%; text-align: center;\"\u003e2ml\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 31.982%; text-align: center;\"\u003e10ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. This product is only for in vitro testing, and cannot be edible or injected; Please keep away from the reach of children.\u003cbr\u003e2. Prepare and use it now. Once the nanoparticles are resuspended with culture medium or PBS, immediately add them to the cells to be tested and mix.\u003cbr\u003e3. During use, the fluorescent probes of different antibodies used can be freely matched as needed.\u003cbr\u003e4. Please place the fixed cells at 4 °C and complete the test within one week.\u003cbr\u003e5. This product is only suitable for testing mouse samples.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFreeze-dry storage at-20 ℃ ~-80 ℃, valid for 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"20T","offer_id":41683115540555,"sku":null,"price":171.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":41683115573323,"sku":null,"price":679.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_347e1d88-59d4-4c5d-a633-749be21e6411.png?v=1770720276"},{"product_id":"tumor-antigen-specific-t-cell-content-detection-kit-abs57007","title":"MC38 tumor antigen-specific T cell content detection stimulation kit for colorectal cancer mice","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eMethods of use and testing procedures:\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003e1. Add the cells to be detected and the corresponding culture medium to each well of the cell culture dish or cell culture plate, and the cell concentration is 0.8 × 10\u003csup\u003e6\u003c\/sup\u003e-5×10\u003csup\u003e6\u003c\/sup\u003eCells\/mL. Considering the cell loss and the accuracy of flow cytometry detection, it is recommended to use at least 1 million cells. It is recommended to use 12-well or 24-well plate culture, and use at least 1-2mL of culture medium.\u003c\/p\u003e\n\u003cp\u003e2. Add nanoparticles resuspended with PBS or corresponding medium thereto. (The final concentration of nanoparticles used in this test kit is 0.5 mg\/mL-1. 0 mg\/mL)\u003c\/p\u003e\n\u003cp\u003e3. The nanoparticles were incubated with the cells to be detected for 32-48 hours.\u003c\/p\u003e\n\u003cp\u003e4. Add Brefeldin A Solution (BFA) (1000 × diluted into cell culture medium) to the culture medium and incubate for a total of 4 h.\u003c\/p\u003e\n\u003cp\u003e5. Use a pipette to pipette the suspended cells in the collection medium. After collecting the cells, centrifuge the sample cells at 400-500g for 5min. Discard the supernatant and collect the cells.\u003c\/p\u003e\n\u003cp\u003e6. After collecting the cells, resuspend the sample cells in 1 mL PBS.\u003c\/p\u003e\n\u003cp\u003e7. In addition to the blank control, add 2μL of live and dead cell dye to each sample to label live cells and dead cells, mix evenly, and incubate on ice for 15-30 minutes in the dark.\u003c\/p\u003e\n\u003cp\u003e8. After incubation, centrifuge at 400-500g for 5 min, and resuspend the cells with 100μL FACS buffer.\u003c\/p\u003e\n\u003cp\u003e9. Except for the blank control. Add 2 μL Fc Block to each sample to block the Fc receptor on the surface of immune cells, mix well and incubate on ice for 5 min.\u003c\/p\u003e\n\u003cp\u003e10. After incubation, add corresponding membrane-labeled antibodies CD3 antibody, CD4 antibody, and CD8 antibody labeled with different fluorescent dyes to each tube, and incubate on ice for 15-30min.\u003c\/p\u003e\n\u003cp\u003e11. Add 500μL of 4% paraformaldehyde directly to fix the cells and incubate on ice for 40-60min.\u003c\/p\u003e\n\u003cp\u003e12. Centrifuge at 400-500g for 5min, discard the supernatant, then add 100μL of membrane breaking agent to resuspend the cells, and incubate on ice for 30min.\u003c\/p\u003e\n\u003cp\u003e13. After incubation, add the corresponding fluorescent dye-labeled IFN-γ antibody to each tube and incubate on ice for 30 minutes.\u003c\/p\u003e\n\u003cp\u003e14. Centrifuge at 400-500g for 5min, discard the supernatant, resuspend the cells with 200μL FACS buffer or fix the cells with 500μL 4% paraformaldehyde, incubate on ice for 40-60min before detection.\u003c\/p\u003e\n\u003cp\u003e15. Use flow cytometry to detect the proportion of cells that are positive for the labeled antibody. (Note: It is necessary to pass through 400 mesh filter cloth before testing on the machine)\u003c\/p\u003e\n\u003cp\u003e16. Use flow cytometry analysis software to analyze the proportion of CD3 + IFN-γ +, CD3+CD4 + IFN-γ + and CD3+CD8 + IFN-γ + T cells in living cells to the corresponding cells, that is, the proportion and content of tumor antigen-specific T cells (the more cells, the more accurate the detection. It is recommended that the number of living cells collected during detection is more than 100,000, and the number of living cells collected is preferably more than 200,000).\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003eTumor antigen-specific T cells are the main force in killing cancer cells. Due to the high heterogeneity of cancer cells and tumor antigens, cancer cells do not have one or more dominant antigens, so there are few dominant antigen-specific T cells. However, the traditional use of several polypeptide antigens or MHC tetramer technology to detect tumor antigen-specific T cells can only detect antigen-specific T cells against several known specific antigens.\u003c\/p\u003e\n\u003cp\u003eThis product detects the whole cell antigens of cancer cells loaded with nanoparticles. The types of antigens are comprehensive, diverse and broad-spectrum, and can comprehensively and accurately detect tumor antigen-specific T cells in different tissues. It has the advantages of high specificity, high accuracy, comprehensive types of tumor antigen-specific T cells detected, and the like.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTest samples:\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003e1. The content of tumor antigen-specific T cells in peripheral blood immune cells of mice;\u003c\/p\u003e\n\u003cp\u003e2. The content of tumor antigen-specific T cells in mouse spleen cells;\u003c\/p\u003e\n\u003cp\u003e3. The content of tumor antigen-specific T cells in mouse lymph node cells;\u003c\/p\u003e\n\u003cp\u003e4. The content of tumor antigen-specific T cells in mouse tumor tissue cells;\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"border-collapse: collapse; width: 35.5212%; height: 131px;\" border=\"1\" width=\"310.52pt\" cellspacing=\"0\" cellpadding=\"0\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et2\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" width=\"139\" height=\"33\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 32.1992%; text-align: center;\" width=\"138\"\u003e\u003cstrong\u003e20T\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 31.982%; text-align: center;\" width=\"137\"\u003e\u003cstrong\u003e100T\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et3\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" height=\"33\"\u003eNanoparticles\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 32.1992%; text-align: center;\"\u003e20mg\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 31.982%; text-align: center;\"\u003e100mg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et4\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" height=\"33\"\u003ePBS\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 32.1992%; text-align: center;\"\u003e2ml\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 31.982%; text-align: center;\"\u003e10ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. This product is only for in vitro testing, and cannot be edible or injected; Please keep away from the reach of children.\u003cbr\u003e2. Prepare and use it now. Once the nanoparticles are resuspended with culture medium or PBS, immediately add them to the cells to be tested and mix.\u003cbr\u003e3. During use, the fluorescent probes of different antibodies used can be freely matched as needed.\u003cbr\u003e4. Please place the fixed cells at 4 °C and complete the test within one week.\u003cbr\u003e5. This product is only suitable for testing mouse samples.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFreeze-dry storage at-20 ℃ ~-80 ℃, valid for 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"20T","offer_id":41683115606091,"sku":null,"price":171.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":41683115638859,"sku":null,"price":679.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_8079580c-45a9-4b8f-a8ab-2b59df5ee332.png?v=1770720279"},{"product_id":"tumor-antigen-specific-t-cell-content-detection-kit-abs57008","title":"MC38-OVA colorectal cancer Mouse tumor antigen-specific T cell content detection stimulation kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eMethods of use and testing procedures:\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003e1. Add the cells to be detected and the corresponding culture medium to each well of the cell culture dish or cell culture plate, and the cell concentration is 0.8 × 10\u003csup\u003e6\u003c\/sup\u003e-5×10\u003csup\u003e6\u003c\/sup\u003eCells\/mL. Considering the cell loss and the accuracy of flow cytometry detection, it is recommended to use at least 1 million cells. It is recommended to use 12-well or 24-well plate culture, and use at least 1-2mL of culture medium.\u003c\/p\u003e\n\u003cp\u003e2. Add nanoparticles resuspended with PBS or corresponding medium thereto. (The final concentration of nanoparticles used in this test kit is 0.5 mg\/mL-1. 0 mg\/mL)\u003c\/p\u003e\n\u003cp\u003e3. The nanoparticles were incubated with the cells to be detected for 32-48 hours.\u003c\/p\u003e\n\u003cp\u003e4. Add Brefeldin A Solution (BFA) (1000 × diluted into cell culture medium) to the culture medium and incubate for a total of 4 h.\u003c\/p\u003e\n\u003cp\u003e5. Use a pipette to pipette the suspended cells in the collection medium. After collecting the cells, centrifuge the sample cells at 400-500g for 5min. Discard the supernatant and collect the cells.\u003c\/p\u003e\n\u003cp\u003e6. After collecting the cells, resuspend the sample cells in 1 mL PBS.\u003c\/p\u003e\n\u003cp\u003e7. In addition to the blank control, add 2μL of live and dead cell dye to each sample to label live cells and dead cells, mix evenly, and incubate on ice for 15-30 minutes in the dark.\u003c\/p\u003e\n\u003cp\u003e8. After incubation, centrifuge at 400-500g for 5 min, and resuspend the cells with 100μL FACS buffer.\u003c\/p\u003e\n\u003cp\u003e9. Except for the blank control. Add 2 μL Fc Block to each sample to block the Fc receptor on the surface of immune cells, mix well and incubate on ice for 5 min.\u003c\/p\u003e\n\u003cp\u003e10. After incubation, add corresponding membrane-labeled antibodies CD3 antibody, CD4 antibody, and CD8 antibody labeled with different fluorescent dyes to each tube, and incubate on ice for 15-30min.\u003c\/p\u003e\n\u003cp\u003e11. Add 500μL of 4% paraformaldehyde directly to fix the cells and incubate on ice for 40-60min.\u003c\/p\u003e\n\u003cp\u003e12. Centrifuge at 400-500g for 5min, discard the supernatant, then add 100μL of membrane breaking agent to resuspend the cells, and incubate on ice for 30min.\u003c\/p\u003e\n\u003cp\u003e13. After incubation, add the corresponding fluorescent dye-labeled IFN-γ antibody to each tube and incubate on ice for 30 minutes.\u003c\/p\u003e\n\u003cp\u003e14. Centrifuge at 400-500g for 5min, discard the supernatant, resuspend the cells with 200μL FACS buffer or fix the cells with 500μL 4% paraformaldehyde, incubate on ice for 40-60min before detection.\u003c\/p\u003e\n\u003cp\u003e15. Use flow cytometry to detect the proportion of cells that are positive for the labeled antibody. (Note: It is necessary to pass through 400 mesh filter cloth before testing on the machine)\u003c\/p\u003e\n\u003cp\u003e16. Use flow cytometry analysis software to analyze the proportion of CD3 + IFN-γ +, CD3+CD4 + IFN-γ + and CD3+CD8 + IFN-γ + T cells in living cells to the corresponding cells, that is, the proportion and content of tumor antigen-specific T cells (the more cells, the more accurate the detection. It is recommended that the number of living cells collected during detection is more than 100,000, and the number of living cells collected is preferably more than 200,000).\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003eTumor antigen-specific T cells are the main force in killing cancer cells. Due to the high heterogeneity of cancer cells and tumor antigens, cancer cells do not have one or more dominant antigens, so there are few dominant antigen-specific T cells. However, the traditional use of several polypeptide antigens or MHC tetramer technology to detect tumor antigen-specific T cells can only detect antigen-specific T cells against several known specific antigens.\u003c\/p\u003e\n\u003cp\u003eThis product detects the whole cell antigen of cancer cells loaded with nanoparticles. The types of antigens are comprehensive, diverse and broad-spectrum, and can comprehensively and accurately detect tumor antigen-specific T cells in different tissues. Has the advantages of high specificity, high accuracy, comprehensive types of tumor antigen-specific T cells detected, and the like.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTest samples:\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003e1. The content of tumor antigen-specific T cells in peripheral blood immune cells of mice;\u003c\/p\u003e\n\u003cp\u003e2. The content of tumor antigen-specific T cells in mouse spleen cells;\u003c\/p\u003e\n\u003cp\u003e3. The content of tumor antigen-specific T cells in mouse lymph node cells;\u003c\/p\u003e\n\u003cp\u003e4. The content of tumor antigen-specific T cells in mouse tumor tissue cells;\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"border-collapse: collapse; width: 35.5212%; height: 131px;\" border=\"1\" width=\"310.52pt\" cellspacing=\"0\" cellpadding=\"0\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et2\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" width=\"139\" height=\"33\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 32.1992%; text-align: center;\" width=\"138\"\u003e\u003cstrong\u003e20T\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 31.982%; text-align: center;\" width=\"137\"\u003e\u003cstrong\u003e100T\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et3\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" height=\"33\"\u003eNanoparticles\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 32.1992%; text-align: center;\"\u003e20mg\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 31.982%; text-align: center;\"\u003e100mg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et4\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" height=\"33\"\u003ePBS\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 32.1992%; text-align: center;\"\u003e2ml\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 31.982%; text-align: center;\"\u003e10ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. This product is only for in vitro testing, and cannot be edible or injected; Please keep away from the reach of children.\u003cbr\u003e2. Prepare and use it now. Once the nanoparticles are resuspended with culture medium or PBS, immediately add them to the cells to be tested and mix.\u003cbr\u003e3. During use, the fluorescent probes of different antibodies used can be freely matched as needed.\u003cbr\u003e4. Please place the fixed cells at 4 °C and complete the test within one week.\u003cbr\u003e5. This product is only suitable for testing mouse samples.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFreeze-dry storage at-20 ℃ ~-80 ℃, valid for 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"20T","offer_id":41683115671627,"sku":null,"price":171.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":41683115704395,"sku":null,"price":679.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_0862e861-18e5-4eb7-81e2-24dc89a4098e.png?v=1770720277"},{"product_id":"tumor-antigen-specific-t-cell-content-detection-kit-abs57009","title":"MC38-Fluc Mouse Tumor Antigen-specific T Cell Content Detection Kit for Colorectal Cancer","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eMethods of use and testing procedures:\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003e1. Add the cells to be detected and the corresponding culture medium to each well of the cell culture dish or cell culture plate, and the cell concentration is 0.8 × 10\u003csup\u003e6\u003c\/sup\u003e-5×10\u003csup\u003e6\u003c\/sup\u003eCells\/mL. Considering the cell loss and the accuracy of flow cytometry detection, it is recommended to use at least 1 million cells. It is recommended to use 12-well or 24-well plate culture, and use at least 1-2mL of culture medium.\u003c\/p\u003e\n\u003cp\u003e2. Add nanoparticles resuspended with PBS or corresponding medium thereto. (The final concentration of nanoparticles used in this test kit is 0.5 mg\/mL-1. 0 mg\/mL)\u003c\/p\u003e\n\u003cp\u003e3. The nanoparticles were incubated with the cells to be detected for 32-48 hours.\u003c\/p\u003e\n\u003cp\u003e4. Add Brefeldin A Solution (BFA) (1000 × diluted into cell culture medium) to the culture medium and incubate for a total of 4 h.\u003c\/p\u003e\n\u003cp\u003e5. Use a pipette to pipette the suspended cells in the collection medium. After collecting the cells, centrifuge the sample cells at 400-500g for 5min. Discard the supernatant and collect the cells.\u003c\/p\u003e\n\u003cp\u003e6. After collecting the cells, resuspend the sample cells in 1 mL PBS.\u003c\/p\u003e\n\u003cp\u003e7. In addition to the blank control, add 2μL of live and dead cell dye to each sample to label live cells and dead cells, mix evenly, and incubate on ice for 15-30 minutes in the dark.\u003c\/p\u003e\n\u003cp\u003e8. After incubation, centrifuge at 400-500g for 5 min, and resuspend the cells with 100μL FACS buffer.\u003c\/p\u003e\n\u003cp\u003e9. Except for the blank control. Add 2 μL Fc Block to each sample to block the Fc receptor on the surface of immune cells, mix well and incubate on ice for 5 min.\u003c\/p\u003e\n\u003cp\u003e10. After incubation, add corresponding membrane-labeled antibodies CD3 antibody, CD4 antibody, and CD8 antibody labeled with different fluorescent dyes to each tube, and incubate on ice for 15-30min.\u003c\/p\u003e\n\u003cp\u003e11. Add 500μL of 4% paraformaldehyde directly to fix the cells and incubate on ice for 40-60min.\u003c\/p\u003e\n\u003cp\u003e12. Centrifuge at 400-500g for 5min, discard the supernatant, then add 100μL of membrane breaking agent to resuspend the cells, and incubate on ice for 30min.\u003c\/p\u003e\n\u003cp\u003e13. After incubation, add the corresponding fluorescent dye-labeled IFN-γ antibody to each tube and incubate on ice for 30 minutes.\u003c\/p\u003e\n\u003cp\u003e14. Centrifuge at 400-500g for 5min, discard the supernatant, resuspend the cells with 200μL FACS buffer or fix the cells with 500μL 4% paraformaldehyde, incubate on ice for 40-60min before detection.\u003c\/p\u003e\n\u003cp\u003e15. Use flow cytometry to detect the proportion of cells that are positive for the labeled antibody. (Note: It is necessary to pass through 400 mesh filter cloth before testing on the machine)\u003c\/p\u003e\n\u003cp\u003e16. Use flow cytometry analysis software to analyze the proportion of CD3 + IFN-γ +, CD3+CD4 + IFN-γ + and CD3+CD8 + IFN-γ + T cells in living cells to the corresponding cells, that is, the proportion and content of tumor antigen-specific T cells (the more cells, the more accurate the detection. It is recommended that the number of living cells collected during detection is more than 100,000, and the number of living cells collected is preferably more than 200,000).\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003eTumor antigen-specific T cells are the main force in killing cancer cells. Due to the high heterogeneity of cancer cells and tumor antigens, cancer cells do not have one or more dominant antigens, so there are few dominant antigen-specific T cells. However, the traditional use of several polypeptide antigens or MHC tetramer technology to detect tumor antigen-specific T cells can only detect antigen-specific T cells against several known specific antigens.\u003c\/p\u003e\n\u003cp\u003eThis product detects the whole cell antigens of cancer cells loaded with nanoparticles. The types of antigens are comprehensive, diverse and broad-spectrum, and can comprehensively and accurately detect tumor antigen-specific T cells in different tissues. It has the advantages of high specificity, high accuracy, comprehensive types of tumor antigen-specific T cells detected, and the like.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTest samples:\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003e1. The content of tumor antigen-specific T cells in peripheral blood immune cells of mice;\u003c\/p\u003e\n\u003cp\u003e2. The content of tumor antigen-specific T cells in mouse spleen cells;\u003c\/p\u003e\n\u003cp\u003e3. The content of tumor antigen-specific T cells in mouse lymph node cells;\u003c\/p\u003e\n\u003cp\u003e4. The content of tumor antigen-specific T cells in mouse tumor tissue cells;\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"border-collapse: collapse; width: 35.5212%; height: 131px;\" border=\"1\" width=\"310.52pt\" cellspacing=\"0\" cellpadding=\"0\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et2\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" width=\"139\" height=\"33\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 32.1992%; text-align: center;\" width=\"138\"\u003e\u003cstrong\u003e20T\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 31.982%; text-align: center;\" width=\"137\"\u003e\u003cstrong\u003e100T\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et3\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" height=\"33\"\u003eNanoparticles\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 32.1992%; text-align: center;\"\u003e20mg\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 31.982%; text-align: center;\"\u003e100mg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et4\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" height=\"33\"\u003ePBS\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 32.1992%; text-align: center;\"\u003e2ml\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 31.982%; text-align: center;\"\u003e10ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. This product is only for in vitro testing, and cannot be edible or injected; Please keep away from the reach of children.\u003cbr\u003e2. Prepare and use it now. Once the nanoparticles are resuspended with culture medium or PBS, immediately add them to the cells to be tested and mix.\u003cbr\u003e3. During use, the fluorescent probes of different antibodies used can be freely matched as needed.\u003cbr\u003e4. Please place the fixed cells at 4 °C and complete the test within one week.\u003cbr\u003e5. This product is only suitable for testing mouse samples.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFreeze-dry storage at-20 ℃ ~-80 ℃, valid for 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"20T","offer_id":41683115737163,"sku":null,"price":171.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":41683115769931,"sku":null,"price":679.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_480ddbab-ab50-4c65-b1f5-b14060d6826a.png?v=1770720276"},{"product_id":"tumor-antigen-specific-t-cell-content-detection-kit-abs57010","title":"Pan02 Pancreatic Cancer Mouse Tumor Antigen-specific T Cell Content Detection Stimulation Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eMethods of use and testing procedures:\u003c\/strong\u003e?\u003c\/p\u003e\n\u003cp\u003e1. Add the cells to be detected and the corresponding culture medium to each well of the cell culture dish or cell culture plate, and the cell concentration is 0.8 × 10\u003csup\u003e6\u003c\/sup\u003e-5×10\u003csup\u003e6\u003c\/sup\u003eCells\/mL. Considering the cell loss and the accuracy of flow cytometry detection, it is recommended to use at least 1 million cells. It is recommended to use 12-well or 24-well plate culture, and use at least 1-2mL of culture medium.\u003c\/p\u003e\n\u003cp\u003e2. Add nanoparticles resuspended with PBS or corresponding medium thereto. (The final concentration of nanoparticles used in this test kit is 0.5 mg\/mL-1. 0 mg\/mL)\u003c\/p\u003e\n\u003cp\u003e3. The nanoparticles were incubated with the cells to be detected for 32-48 hours.\u003c\/p\u003e\n\u003cp\u003e4. Add Brefeldin A Solution (BFA) (1000 × diluted into cell culture medium) to the culture medium and incubate for a total of 4 h.\u003c\/p\u003e\n\u003cp\u003e5. Use a pipette to pipette the suspended cells in the collection medium. After collecting the cells, centrifuge the sample cells at 400-500g for 5min. Discard the supernatant and collect the cells.\u003c\/p\u003e\n\u003cp\u003e6. After collecting the cells, resuspend the sample cells in 1 mL PBS.\u003c\/p\u003e\n\u003cp\u003e7. In addition to the blank control, add 2μL of live and dead cell dye to each sample to label live cells and dead cells, mix evenly, and incubate on ice for 15-30 minutes in the dark.\u003c\/p\u003e\n\u003cp\u003e8. After incubation, centrifuge at 400-500g for 5 min, and resuspend the cells with 100μL FACS buffer.\u003c\/p\u003e\n\u003cp\u003e9. Except for the blank control. Add 2 μL Fc Block to each sample to block the Fc receptor on the surface of immune cells, mix well and incubate on ice for 5 min.\u003c\/p\u003e\n\u003cp\u003e10. After incubation, add corresponding membrane-labeled antibodies CD3 antibody, CD4 antibody, and CD8 antibody labeled with different fluorescent dyes to each tube, and incubate on ice for 15-30min.\u003c\/p\u003e\n\u003cp\u003e11. Add 500μL of 4% paraformaldehyde directly to fix the cells and incubate on ice for 40-60min.\u003c\/p\u003e\n\u003cp\u003e12. Centrifuge at 400-500g for 5min, discard the supernatant, then add 100μL of membrane breaking agent to resuspend the cells, and incubate on ice for 30min.\u003c\/p\u003e\n\u003cp\u003e13. After incubation, add the corresponding fluorescent dye-labeled IFN-γ antibody to each tube and incubate on ice for 30 minutes.\u003c\/p\u003e\n\u003cp\u003e14. Centrifuge at 400-500g for 5min, discard the supernatant, resuspend the cells with 200μL FACS buffer or fix the cells with 500μL 4% paraformaldehyde, incubate on ice for 40-60min before detection.\u003c\/p\u003e\n\u003cp\u003e15. Use flow cytometry to detect the proportion of cells that are positive for the labeled antibody. (Note: It is necessary to pass through 400 mesh filter cloth before testing on the machine)\u003c\/p\u003e\n\u003cp\u003e16. Use flow cytometry analysis software to analyze the proportion of CD3 + IFN-γ +, CD3+CD4 + IFN-γ + and CD3+CD8 + IFN-γ + T cells in living cells to the corresponding cells, that is, the proportion and content of tumor antigen-specific T cells (the more cells, the more accurate the detection. It is recommended that the number of living cells collected during detection is more than 100,000, and the number of living cells collected is preferably more than 200,000).\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e?\u003c\/p\u003e\n\u003cp\u003eTumor antigen-specific T cells are the main force in killing cancer cells. Due to the high heterogeneity of cancer cells and tumor antigens, cancer cells do not have one or more dominant antigens, so there are few dominant antigen-specific T cells. However, the traditional use of several polypeptide antigens or MHC tetramer technology to detect tumor antigen-specific T cells can only detect antigen-specific T cells against several known specific antigens.\u003c\/p\u003e\n\u003cp\u003eThis product detects the whole cell antigen of cancer cells loaded with nanoparticles. The types of antigens are comprehensive, diverse and broad-spectrum, and can comprehensively and accurately detect tumor antigen-specific T cells in different tissues. It has the advantages of high specificity, high accuracy, comprehensive types of tumor antigen-specific T cells detected, and the like.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTest samples:\u003c\/strong\u003e?\u003c\/p\u003e\n\u003cp\u003e1. The content of tumor antigen-specific T cells in peripheral blood immune cells of mice;\u003c\/p\u003e\n\u003cp\u003e2. The content of tumor antigen-specific T cells in mouse spleen cells;\u003c\/p\u003e\n\u003cp\u003e3. The content of tumor antigen-specific T cells in mouse lymph node cells;\u003c\/p\u003e\n\u003cp\u003e4. The content of tumor antigen-specific T cells in mouse tumor tissue cells;\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"border-collapse: collapse; width: 35.5212%; height: 131px;\" border=\"1\" width=\"310.52pt\" cellspacing=\"0\" cellpadding=\"0\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et2\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" width=\"139\" height=\"33\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 32.1992%; text-align: center;\" width=\"138\"\u003e\u003cstrong\u003e20T\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 31.982%; text-align: center;\" width=\"137\"\u003e\u003cstrong\u003e100T\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et3\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" height=\"33\"\u003eNanoparticles\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 32.1992%; text-align: center;\"\u003e20mg\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 31.982%; text-align: center;\"\u003e100mg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et4\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" height=\"33\"\u003ePBS\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 32.1992%; text-align: center;\"\u003e2ml\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 31.982%; text-align: center;\"\u003e10ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFreeze-dry storage at-20 ℃ ~-80 ℃, valid for 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"20T","offer_id":41683115802699,"sku":null,"price":171.0,"currency_code":"USD","in_stock":true}]},{"product_id":"tumor-antigen-specific-t-cell-content-detection-kit-abs57011","title":"Pan02 Tumor Antigen Specific T Cell Content Detection Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eMethods of use and testing procedures:\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003e1. Add the cells to be detected and the corresponding culture medium to each well of the cell culture dish or cell culture plate, and the cell concentration is 0.8 × 10\u003csup\u003e6\u003c\/sup\u003e-5×10\u003csup\u003e6\u003c\/sup\u003eCells\/mL. Considering the cell loss and the accuracy of flow cytometry detection, it is recommended to use at least 1 million cells. It is recommended to use 12-well or 24-well plate culture, and use at least 1-2mL of culture medium.\u003c\/p\u003e\n\u003cp\u003e2. Add nanoparticles resuspended with PBS or corresponding medium thereto. (The final concentration of nanoparticles used in this test kit is 0.5 mg\/mL-1. 0 mg\/mL)\u003c\/p\u003e\n\u003cp\u003e3. The nanoparticles were incubated with the cells to be detected for 32-48 hours.\u003c\/p\u003e\n\u003cp\u003e4. Add Brefeldin A Solution (BFA) (1000 × diluted into cell culture medium) to the culture medium and incubate for a total of 4 h.\u003c\/p\u003e\n\u003cp\u003e5. Use a pipette to pipette the suspended cells in the collection medium. After collecting the cells, centrifuge the sample cells at 400-500g for 5min. Discard the supernatant and collect the cells.\u003c\/p\u003e\n\u003cp\u003e6. After collecting the cells, resuspend the sample cells in 1 mL PBS.\u003c\/p\u003e\n\u003cp\u003e7. In addition to the blank control, add 2μL of live and dead cell dye to each sample to label live cells and dead cells, mix evenly, and incubate on ice for 15-30 minutes in the dark.\u003c\/p\u003e\n\u003cp\u003e8. After incubation, centrifuge at 400-500g for 5 min, and resuspend the cells with 100μL FACS buffer.\u003c\/p\u003e\n\u003cp\u003e9. Except for the blank control. Add 2 μL Fc Block to each sample to block the Fc receptor on the surface of immune cells, mix well and incubate on ice for 5 min.\u003c\/p\u003e\n\u003cp\u003e10. After incubation, add corresponding membrane-labeled antibodies CD3 antibody, CD4 antibody, and CD8 antibody labeled with different fluorescent dyes to each tube, and incubate on ice for 15-30min.\u003c\/p\u003e\n\u003cp\u003e11. Add 500μL of 4% paraformaldehyde directly to fix the cells and incubate on ice for 40-60min.\u003c\/p\u003e\n\u003cp\u003e12. Centrifuge at 400-500g for 5min, discard the supernatant, then add 100μL of membrane breaking agent to resuspend the cells, and incubate on ice for 30min.\u003c\/p\u003e\n\u003cp\u003e13. After incubation, add the corresponding fluorescent dye-labeled IFN-γ antibody to each tube and incubate on ice for 30 minutes.\u003c\/p\u003e\n\u003cp\u003e14. Centrifuge at 400-500g for 5min, discard the supernatant, resuspend the cells with 200μL FACS buffer or fix the cells with 500μL 4% paraformaldehyde, incubate on ice for 40-60min before detection.\u003c\/p\u003e\n\u003cp\u003e15. Use flow cytometry to detect the proportion of cells that are positive for the labeled antibody. (Note: It is necessary to pass through 400 mesh filter cloth before testing on the machine)\u003c\/p\u003e\n\u003cp\u003e16. Use flow cytometry analysis software to analyze the proportion of CD3 + IFN-γ +, CD3+CD4 + IFN-γ + and CD3+CD8 + IFN-γ + T cells in living cells to the corresponding cells, that is, the proportion and content of tumor antigen-specific T cells (the more cells, the more accurate the detection. It is recommended that the number of living cells collected during detection is more than 100,000, and the number of living cells collected is preferably more than 200,000).\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003eTumor antigen-specific T cells are the main force in killing cancer cells. Due to the high heterogeneity of cancer cells and tumor antigens, cancer cells do not have one or more dominant antigens, so there are few dominant antigen-specific T cells. However, the traditional use of several polypeptide antigens or MHC tetramer technology to detect tumor antigen-specific T cells can only detect antigen-specific T cells against several known specific antigens.\u003c\/p\u003e\n\u003cp\u003eThis product detects the whole cell antigens of cancer cells loaded with nanoparticles. The types of antigens are comprehensive, diverse and broad-spectrum, and can comprehensively and accurately detect tumor antigen-specific T cells in different tissues. It has the advantages of high specificity, high accuracy, comprehensive types of tumor antigen-specific T cells detected, and the like.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTest samples:\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003e1. The content of tumor antigen-specific T cells in peripheral blood immune cells of mice;\u003c\/p\u003e\n\u003cp\u003e2. The content of tumor antigen-specific T cells in mouse spleen cells;\u003c\/p\u003e\n\u003cp\u003e3. The content of tumor antigen-specific T cells in mouse lymph node cells;\u003c\/p\u003e\n\u003cp\u003e4. The content of tumor antigen-specific T cells in mouse tumor tissue cells;\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"border-collapse: collapse; width: 35.5212%; height: 131px;\" border=\"1\" width=\"310.52pt\" cellspacing=\"0\" cellpadding=\"0\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et2\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" width=\"139\" height=\"33\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 32.1992%; text-align: center;\" width=\"138\"\u003e\u003cstrong\u003e20T\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 31.982%; text-align: center;\" width=\"137\"\u003e\u003cstrong\u003e100T\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et3\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" height=\"33\"\u003eNanoparticles\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 32.1992%; text-align: center;\"\u003e20mg\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 31.982%; text-align: center;\"\u003e100mg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et4\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" height=\"33\"\u003ePBS\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 32.1992%; text-align: center;\"\u003e2ml\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 31.982%; text-align: center;\"\u003e10ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. This product is only for in vitro testing, and cannot be edible or injected; Please keep away from the reach of children.\u003cbr\u003e2. Prepare and use it now. Once the nanoparticles are resuspended with culture medium or PBS, immediately add them to the cells to be tested and mix.\u003cbr\u003e3. During use, the fluorescent probes of different antibodies used can be freely matched as needed.\u003cbr\u003e4. Please place the fixed cells at 4 °C and complete the test within one week.\u003cbr\u003e5. This product is only suitable for testing mouse samples.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFreeze-dry storage at-20 ℃ ~-80 ℃, valid for 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"20T","offer_id":41683115901003,"sku":null,"price":171.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":41683115933771,"sku":null,"price":679.0,"currency_code":"USD","in_stock":true}]},{"product_id":"tumor-antigen-specific-t-cell-content-detection-kit-abs57012","title":"KPC pancreatic cancer mouse tumor antigen-specific T cell content detection stimulation kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eMethods of use and testing procedures:\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003e1. Add the cells to be detected and the corresponding culture medium to each well of the cell culture dish or cell culture plate, and the cell concentration is 0.8 × 10\u003csup\u003e6\u003c\/sup\u003e-5×10\u003csup\u003e6\u003c\/sup\u003eCells\/mL. Considering the cell loss and the accuracy of flow cytometry detection, it is recommended to use at least 1 million cells. It is recommended to use 12-well or 24-well plate culture, and use at least 1-2mL of culture medium.\u003c\/p\u003e\n\u003cp\u003e2. Add nanoparticles resuspended with PBS or corresponding medium thereto. (The final concentration of nanoparticles used in this test kit is 0.5 mg\/mL-1. 0 mg\/mL)\u003c\/p\u003e\n\u003cp\u003e3. The nanoparticles were incubated with the cells to be detected for 32-48 hours.\u003c\/p\u003e\n\u003cp\u003e4. Add Brefeldin A Solution (BFA) (1000 × diluted into cell culture medium) to the culture medium and incubate for a total of 4 h.\u003c\/p\u003e\n\u003cp\u003e5. Use a pipette to pipette the suspended cells in the collection medium. After collecting the cells, centrifuge the sample cells at 400-500g for 5min. Discard the supernatant and collect the cells.\u003c\/p\u003e\n\u003cp\u003e6. After collecting the cells, resuspend the sample cells in 1 mL PBS.\u003c\/p\u003e\n\u003cp\u003e7. In addition to the blank control, add 2μL of live and dead cell dye to each sample to label live cells and dead cells, mix evenly, and incubate on ice for 15-30 minutes in the dark.\u003c\/p\u003e\n\u003cp\u003e8. After incubation, centrifuge at 400-500g for 5 min, and resuspend the cells with 100μL FACS buffer.\u003c\/p\u003e\n\u003cp\u003e9. Except for the blank control. Add 2 μL Fc Block to each sample to block the Fc receptor on the surface of immune cells, mix well and incubate on ice for 5 min.\u003c\/p\u003e\n\u003cp\u003e10. After incubation, add corresponding membrane-labeled antibodies CD3 antibody, CD4 antibody, and CD8 antibody labeled with different fluorescent dyes to each tube, and incubate on ice for 15-30min.\u003c\/p\u003e\n\u003cp\u003e11. Add 500μL of 4% paraformaldehyde directly to fix the cells and incubate on ice for 40-60min.\u003c\/p\u003e\n\u003cp\u003e12. Centrifuge at 400-500g for 5min, discard the supernatant, then add 100μL of membrane breaking agent to resuspend the cells, and incubate on ice for 30min.\u003c\/p\u003e\n\u003cp\u003e13. After incubation, add the corresponding fluorescent dye-labeled IFN-γ antibody to each tube and incubate on ice for 30 minutes.\u003c\/p\u003e\n\u003cp\u003e14. Centrifuge at 400-500g for 5min, discard the supernatant, resuspend the cells with 200μL FACS buffer or fix the cells with 500μL 4% paraformaldehyde, incubate on ice for 40-60min before detection.\u003c\/p\u003e\n\u003cp\u003e15. Use flow cytometry to detect the proportion of cells that are positive for the labeled antibody. (Note: It is necessary to pass through 400 mesh filter cloth before testing on the machine)\u003c\/p\u003e\n\u003cp\u003e16. Use flow cytometry analysis software to analyze the proportion of CD3 + IFN-γ +, CD3+CD4 + IFN-γ + and CD3+CD8 + IFN-γ + T cells in living cells to the corresponding cells, that is, the proportion and content of tumor antigen-specific T cells (the more cells, the more accurate the detection. It is recommended that the number of living cells collected during detection is more than 100,000, and the number of living cells collected is preferably more than 200,000).\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003eTumor antigen-specific T cells are the main force in killing cancer cells. Due to the high heterogeneity of cancer cells and tumor antigens, cancer cells do not have one or more dominant antigens, so there are few dominant antigen-specific T cells. However, the traditional use of several polypeptide antigens or MHC tetramer technology to detect tumor antigen-specific T cells can only detect antigen-specific T cells against several known specific antigens.\u003c\/p\u003e\n\u003cp\u003eThis product detects the whole cell antigens of cancer cells loaded with nanoparticles. The types of antigens are comprehensive, diverse and broad-spectrum, and can comprehensively and accurately detect tumor antigen-specific T cells in different tissues. Has the advantages of high specificity, high accuracy, comprehensive kinds of tumor antigen-specific T cells detected, and the like.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTest samples:\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003e1. The content of tumor antigen-specific T cells in peripheral blood immune cells of mice;\u003c\/p\u003e\n\u003cp\u003e2. The content of tumor antigen-specific T cells in mouse spleen cells;\u003c\/p\u003e\n\u003cp\u003e3. The content of tumor antigen-specific T cells in mouse lymph node cells;\u003c\/p\u003e\n\u003cp\u003e4. The content of tumor antigen-specific T cells in mouse tumor tissue cells;\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"border-collapse: collapse; width: 35.5212%; height: 131px;\" border=\"1\" width=\"310.52pt\" cellspacing=\"0\" cellpadding=\"0\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et2\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" width=\"139\" height=\"33\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 32.1992%; text-align: center;\" width=\"138\"\u003e\u003cstrong\u003e20T\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 31.982%; text-align: center;\" width=\"137\"\u003e\u003cstrong\u003e100T\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et3\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" height=\"33\"\u003eNanoparticles\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 32.1992%; text-align: center;\"\u003e20mg\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 31.982%; text-align: center;\"\u003e100mg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et4\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" height=\"33\"\u003ePBS\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 32.1992%; text-align: center;\"\u003e2ml\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 31.982%; text-align: center;\"\u003e10ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. This product is only for in vitro testing, and cannot be edible or injected; Please keep away from the reach of children.\u003cbr\u003e2. Prepare and use it now. Once the nanoparticles are resuspended with culture medium or PBS, immediately add them to the cells to be tested and mix.\u003cbr\u003e3. During use, the fluorescent probes of different antibodies used can be freely matched as needed.\u003cbr\u003e4. Please place the fixed cells at 4 °C and complete the test within one week.\u003cbr\u003e5. This product is only suitable for testing mouse samples.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFreeze-dry storage at-20 ℃ ~-80 ℃, valid for 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"20T","offer_id":41683115966539,"sku":null,"price":171.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":41683115999307,"sku":null,"price":679.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_516ccc7e-976a-4017-b16b-3e2517b711ac.png?v=1770720275"},{"product_id":"tumor-antigen-specific-t-cell-content-detection-kit-abs57013","title":"GL261-Fluc brain cancer mouse tumor antigen-specific T cell content detection stimulation kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eMethods of use and testing procedures:\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003e1. Add the cells to be detected and the corresponding culture medium to each well of the cell culture dish or cell culture plate, and the cell concentration is 0.8 × 10\u003csup\u003e6\u003c\/sup\u003e-5×10\u003csup\u003e6\u003c\/sup\u003eCells\/mL. Considering the cell loss and the accuracy of flow cytometry detection, it is recommended to use at least 1 million cells. It is recommended to use 12-well or 24-well plate culture, and use at least 1-2mL of culture medium.\u003c\/p\u003e\n\u003cp\u003e2. Add nanoparticles resuspended with PBS or corresponding medium thereto. (The final concentration of nanoparticles used in this test kit is 0.5 mg\/mL-1. 0 mg\/mL)\u003c\/p\u003e\n\u003cp\u003e3. The nanoparticles were incubated with the cells to be detected for 32-48 hours.\u003c\/p\u003e\n\u003cp\u003e4. Add Brefeldin A Solution (BFA) (1000 × diluted into cell culture medium) to the culture medium and incubate for a total of 4 h.\u003c\/p\u003e\n\u003cp\u003e5. Use a pipette to pipette the suspended cells in the collection medium. After collecting the cells, centrifuge the sample cells at 400-500g for 5min. Discard the supernatant and collect the cells.\u003c\/p\u003e\n\u003cp\u003e6. After collecting the cells, resuspend the sample cells in 1 mL PBS.\u003c\/p\u003e\n\u003cp\u003e7. In addition to the blank control, add 2μL of live and dead cell dye to each sample to label live cells and dead cells, mix evenly, and incubate on ice for 15-30 minutes in the dark.\u003c\/p\u003e\n\u003cp\u003e8. After incubation, centrifuge at 400-500g for 5 min, and resuspend the cells with 100μL FACS buffer.\u003c\/p\u003e\n\u003cp\u003e9. Except for the blank control. Add 2 μL Fc Block to each sample to block the Fc receptor on the surface of immune cells, mix well and incubate on ice for 5 min.\u003c\/p\u003e\n\u003cp\u003e10. After incubation, add corresponding membrane-labeled antibodies CD3 antibody, CD4 antibody, and CD8 antibody labeled with different fluorescent dyes to each tube, and incubate on ice for 15-30min.\u003c\/p\u003e\n\u003cp\u003e11. Add 500μL of 4% paraformaldehyde directly to fix the cells and incubate on ice for 40-60min.\u003c\/p\u003e\n\u003cp\u003e12. Centrifuge at 400-500g for 5min, discard the supernatant, then add 100μL of membrane breaking agent to resuspend the cells, and incubate on ice for 30min.\u003c\/p\u003e\n\u003cp\u003e13. After incubation, add the corresponding fluorescent dye-labeled IFN-γ antibody to each tube and incubate on ice for 30 minutes.\u003c\/p\u003e\n\u003cp\u003e14. Centrifuge at 400-500g for 5min, discard the supernatant, resuspend the cells with 200μL FACS buffer or fix the cells with 500μL 4% paraformaldehyde, incubate on ice for 40-60min before detection.\u003c\/p\u003e\n\u003cp\u003e15. Use flow cytometry to detect the proportion of cells that are positive for the labeled antibody. (Note: It is necessary to pass through 400 mesh filter cloth before testing on the machine)\u003c\/p\u003e\n\u003cp\u003e16. Use flow cytometry analysis software to analyze the proportion of CD3 + IFN-γ +, CD3+CD4 + IFN-γ + and CD3+CD8 + IFN-γ + T cells in living cells to the corresponding cells, that is, the proportion and content of tumor antigen-specific T cells (the more cells, the more accurate the detection. It is recommended that the number of living cells collected during detection is more than 100,000, and the number of living cells collected is preferably more than 200,000).\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003eTumor antigen-specific T cells are the main force in killing cancer cells. Due to the high heterogeneity of cancer cells and tumor antigens, cancer cells do not have one or more dominant antigens, so there are few dominant antigen-specific T cells. However, the traditional use of several polypeptide antigens or MHC tetramer technology to detect tumor antigen-specific T cells can only detect antigen-specific T cells against several known specific antigens.\u003c\/p\u003e\n\u003cp\u003eThis product detects the whole cell antigen of cancer cells loaded with nanoparticles. The types of antigens are comprehensive, diverse and broad-spectrum, and can comprehensively and accurately detect tumor antigen-specific T cells in different tissues. It has the advantages of high specificity, high accuracy, comprehensive types of tumor antigen-specific T cells detected, and the like.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTest samples:\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003e1. The content of tumor antigen-specific T cells in peripheral blood immune cells of mice;\u003c\/p\u003e\n\u003cp\u003e2. The content of tumor antigen-specific T cells in mouse spleen cells;\u003c\/p\u003e\n\u003cp\u003e3. The content of tumor antigen-specific T cells in mouse lymph node cells;\u003c\/p\u003e\n\u003cp\u003e4. The content of tumor antigen-specific T cells in mouse tumor tissue cells;\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"border-collapse: collapse; width: 35.5212%; height: 131px;\" border=\"1\" width=\"310.52pt\" cellspacing=\"0\" cellpadding=\"0\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et2\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" width=\"139\" height=\"33\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 32.1992%; text-align: center;\" width=\"138\"\u003e\u003cstrong\u003e20T\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 31.982%; text-align: center;\" width=\"137\"\u003e\u003cstrong\u003e100T\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et3\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" height=\"33\"\u003eNanoparticles\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 32.1992%; text-align: center;\"\u003e20mg\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 31.982%; text-align: center;\"\u003e100mg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et4\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" height=\"33\"\u003ePBS\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 32.1992%; text-align: center;\"\u003e2ml\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 31.982%; text-align: center;\"\u003e10ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. This product is only for in vitro testing, and cannot be edible or injected; Please keep away from the reach of children.\u003cbr\u003e2. Prepare and use it now. Once the nanoparticles are resuspended with culture medium or PBS, immediately add them to the cells to be tested and mix.\u003cbr\u003e3. During use, the fluorescent probes of different antibodies used can be freely matched as needed.\u003cbr\u003e4. Please place the fixed cells at 4 °C and complete the test within one week.\u003cbr\u003e5. This product is only suitable for testing mouse samples.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFreeze-dry storage at-20 ℃ ~-80 ℃, valid for 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"20T","offer_id":41683116032075,"sku":null,"price":171.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":41683116064843,"sku":null,"price":679.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_13122b15-7c01-4a47-8955-2ebfee0f5809.png?v=1770720274"},{"product_id":"tumor-antigen-specific-t-cell-content-detection-kit-abs57014","title":"GL261 mice brain tumor antigen specific T cells content test stimulus kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eMethods of use and testing procedures:\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003e1. Add the cells to be detected and the corresponding culture medium to each well of the cell culture dish or cell culture plate, and the cell concentration is 0.8 × 10\u003csup\u003e6\u003c\/sup\u003e-5×10\u003csup\u003e6\u003c\/sup\u003eCells\/mL. Considering the cell loss and the accuracy of flow cytometry detection, it is recommended to use at least 1 million cells. It is recommended to use 12-well or 24-well plate culture, and use at least 1-2mL of culture medium.\u003c\/p\u003e\n\u003cp\u003e2. Add nanoparticles resuspended with PBS or corresponding medium thereto. (The final concentration of nanoparticles used in this test kit is 0.5 mg\/mL-1. 0 mg\/mL)\u003c\/p\u003e\n\u003cp\u003e3. The nanoparticles were incubated with the cells to be detected for 32-48 hours.\u003c\/p\u003e\n\u003cp\u003e4. Add Brefeldin A Solution (BFA) (1000 × diluted into cell culture medium) to the culture medium and incubate for a total of 4 h.\u003c\/p\u003e\n\u003cp\u003e5. Use a pipette to pipette the suspended cells in the collection medium. After collecting the cells, centrifuge the sample cells at 400-500g for 5min. Discard the supernatant and collect the cells.\u003c\/p\u003e\n\u003cp\u003e6. After collecting the cells, resuspend the sample cells in 1 mL PBS.\u003c\/p\u003e\n\u003cp\u003e7. In addition to the blank control, add 2μL of live and dead cell dye to each sample to label live cells and dead cells, mix evenly, and incubate on ice for 15-30 minutes in the dark.\u003c\/p\u003e\n\u003cp\u003e8. After incubation, centrifuge at 400-500g for 5 min, and resuspend the cells with 100μL FACS buffer.\u003c\/p\u003e\n\u003cp\u003e9. Except for the blank control. Add 2 μL Fc Block to each sample to block the Fc receptor on the surface of immune cells, mix well and incubate on ice for 5 min.\u003c\/p\u003e\n\u003cp\u003e10. After incubation, add corresponding membrane-labeled antibodies CD3 antibody, CD4 antibody, and CD8 antibody labeled with different fluorescent dyes to each tube, and incubate on ice for 15-30min.\u003c\/p\u003e\n\u003cp\u003e11. Add 500μL of 4% paraformaldehyde directly to fix the cells and incubate on ice for 40-60min.\u003c\/p\u003e\n\u003cp\u003e12. Centrifuge at 400-500g for 5min, discard the supernatant, then add 100μL of membrane breaking agent to resuspend the cells, and incubate on ice for 30min.\u003c\/p\u003e\n\u003cp\u003e13. After incubation, add the corresponding fluorescent dye-labeled IFN-γ antibody to each tube and incubate on ice for 30 minutes.\u003c\/p\u003e\n\u003cp\u003e14. Centrifuge at 400-500g for 5min, discard the supernatant, resuspend the cells with 200μL FACS buffer or fix the cells with 500μL 4% paraformaldehyde, incubate on ice for 40-60min before detection.\u003c\/p\u003e\n\u003cp\u003e15. Use flow cytometry to detect the proportion of cells that are positive for the labeled antibody. (Note: It is necessary to pass through 400 mesh filter cloth before testing on the machine)\u003c\/p\u003e\n\u003cp\u003e16. Use flow cytometry analysis software to analyze the proportion of CD3 + IFN-γ +, CD3+CD4 + IFN-γ + and CD3+CD8 + IFN-γ + T cells in living cells to the corresponding cells, that is, the proportion and content of tumor antigen-specific T cells (the more cells, the more accurate the detection. It is recommended that the number of living cells collected during detection is more than 100,000, and the number of living cells collected is preferably more than 200,000).\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003eTumor antigen-specific T cells are the main force in killing cancer cells. Due to the high heterogeneity of cancer cells and tumor antigens, cancer cells do not have one or more dominant antigens, so there are few dominant antigen-specific T cells. However, the traditional use of several polypeptide antigens or MHC tetramer technology to detect tumor antigen-specific T cells can only detect antigen-specific T cells against several known specific antigens.\u003c\/p\u003e\n\u003cp\u003eThis product detects the whole cell antigens of cancer cells loaded with nanoparticles. The types of antigens are comprehensive, diverse and broad-spectrum, and can comprehensively and accurately detect tumor antigen-specific T cells in different tissues. It has the advantages of high specificity, high accuracy, comprehensive types of tumor antigen-specific T cells detected, and the like.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTest samples:\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003e1. The content of tumor antigen-specific T cells in peripheral blood immune cells of mice;\u003c\/p\u003e\n\u003cp\u003e2. The content of tumor antigen-specific T cells in mouse spleen cells;\u003c\/p\u003e\n\u003cp\u003e3. The content of tumor antigen-specific T cells in mouse lymph node cells;\u003c\/p\u003e\n\u003cp\u003e4. The content of tumor antigen-specific T cells in mouse tumor tissue cells;\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"border-collapse: collapse; width: 35.5212%; height: 131px;\" border=\"1\" width=\"310.52pt\" cellspacing=\"0\" cellpadding=\"0\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et2\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" width=\"139\" height=\"33\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 32.1992%; text-align: center;\" width=\"138\"\u003e\u003cstrong\u003e20T\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 31.982%; text-align: center;\" width=\"137\"\u003e\u003cstrong\u003e100T\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et3\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" height=\"33\"\u003eNanoparticles\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 32.1992%; text-align: center;\"\u003e20mg\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 31.982%; text-align: center;\"\u003e100mg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et4\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" height=\"33\"\u003ePBS\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 32.1992%; text-align: center;\"\u003e2ml\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 31.982%; text-align: center;\"\u003e10ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. This product is only for in vitro testing, and cannot be edible or injected; Please keep away from the reach of children.\u003cbr\u003e2. Prepare and use it now. Once the nanoparticles are resuspended with culture medium or PBS, immediately add them to the cells to be tested and mix.\u003cbr\u003e3. During use, the fluorescent probes of different antibodies used can be freely matched as needed.\u003cbr\u003e4. Please place the fixed cells at 4 °C and complete the test within one week.\u003cbr\u003e5. This product is only suitable for testing mouse samples.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFreeze-dry storage at-20 ℃ ~-80 ℃, valid for 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"20T","offer_id":41683134120011,"sku":null,"price":171.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":41683134152779,"sku":null,"price":679.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_1dab5eee-5e1d-4061-833c-9afe57983cfd.png?v=1770720274"},{"product_id":"tumor-antigen-specific-t-cell-content-detection-kit-abs57015","title":"4T1 Breast Cancer Tumor Antigen Specific T Cells in Mice Content Test Stimulus Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eMethods of use and testing procedures:\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003e1. Add the cells to be detected and the corresponding culture medium to each well of the cell culture dish or cell culture plate, and the cell concentration is 0.8 × 10\u003csup\u003e6\u003c\/sup\u003e-5×10\u003csup\u003e6\u003c\/sup\u003eCells\/mL. Considering the cell loss and the accuracy of flow cytometry detection, it is recommended to use at least 1 million cells. It is recommended to use 12-well or 24-well plate culture, and use at least 1-2mL of culture medium.\u003c\/p\u003e\n\u003cp\u003e2. Add nanoparticles after resuspension with PBS or corresponding medium thereto. (The final concentration of nanoparticles used in this test kit is 0.5 mg\/mL-1. 0 mg\/mL)\u003c\/p\u003e\n\u003cp\u003e3. The nanoparticles were incubated with the cells to be detected for 32-48 hours.\u003c\/p\u003e\n\u003cp\u003e4. Add Brefeldin A Solution (BFA) (1000 × diluted into cell culture medium) to the culture medium and incubate for a total of 4 h.\u003c\/p\u003e\n\u003cp\u003e5. Use a pipette to pipette the suspended cells in the collection medium. After collecting the cells, centrifuge the sample cells at 400-500g for 5min. Discard the supernatant and collect the cells.\u003c\/p\u003e\n\u003cp\u003e6. After collecting the cells, resuspend the sample cells in 1 mL PBS.\u003c\/p\u003e\n\u003cp\u003e7. In addition to the blank control, add 2μL of live and dead cell dye to each sample to label live cells and dead cells, mix evenly, and incubate on ice for 15-30 minutes in the dark.\u003c\/p\u003e\n\u003cp\u003e8. After incubation, centrifuge at 400-500g for 5 min, and resuspend the cells with 100μL FACS buffer.\u003c\/p\u003e\n\u003cp\u003e9. Except for the blank control. Add 2 μL Fc Block to each sample to block the Fc receptor on the surface of immune cells, mix well and incubate on ice for 5 min.\u003c\/p\u003e\n\u003cp\u003e10. After incubation, add corresponding membrane-labeled antibodies CD3 antibody, CD4 antibody, and CD8 antibody labeled with different fluorescent dyes to each tube, and incubate on ice for 15-30min.\u003c\/p\u003e\n\u003cp\u003e11. Add 500μL of 4% paraformaldehyde directly to fix the cells and incubate on ice for 40-60min.\u003c\/p\u003e\n\u003cp\u003e12. Centrifuge at 400-500g for 5min, discard the supernatant, then add 100μL of membrane breaking agent to resuspend the cells, and incubate on ice for 30min.\u003c\/p\u003e\n\u003cp\u003e13. After incubation, add the corresponding fluorescent dye-labeled IFN-γ antibody to each tube and incubate on ice for 30 minutes.\u003c\/p\u003e\n\u003cp\u003e14. Centrifuge at 400-500g for 5min, discard the supernatant, resuspend the cells with 200μL FACS buffer or fix the cells with 500μL 4% paraformaldehyde, incubate on ice for 40-60min before detection.\u003c\/p\u003e\n\u003cp\u003e15. Use flow cytometry to detect the proportion of cells that are positive for the labeled antibody. (Note: It is necessary to pass through 400 mesh filter cloth before testing on the machine)\u003c\/p\u003e\n\u003cp\u003e16. Use flow cytometry analysis software to analyze the proportion of CD3 + IFN-γ +, CD3+CD4 + IFN-γ + and CD3+CD8 + IFN-γ + T cells in living cells to the corresponding cells, that is, the proportion and content of tumor antigen-specific T cells (the more cells, the more accurate the detection. It is recommended that the number of living cells collected during detection is more than 100,000, and the number of living cells collected is preferably more than 200,000).\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003eTumor antigen-specific T cells are the main force in killing cancer cells. Due to the high heterogeneity of cancer cells and tumor antigens, cancer cells do not have one or more dominant antigens, so there are few dominant antigen-specific T cells. However, the traditional use of several polypeptide antigens or MHC tetramer technology to detect tumor antigen-specific T cells can only detect antigen-specific T cells against several known specific antigens.\u003c\/p\u003e\n\u003cp\u003eThis product detects the whole cell antigens of cancer cells loaded with nanoparticles. The types of antigens are comprehensive, diverse and broad-spectrum, and can comprehensively and accurately detect tumor antigen-specific T cells in different tissues. Has the advantages of high specificity, high accuracy, comprehensive kinds of tumor antigen-specific T cells detected, and the like.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTest samples:\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003e1. The content of tumor antigen-specific T cells in peripheral blood immune cells of mice;\u003c\/p\u003e\n\u003cp\u003e2. The content of tumor antigen-specific T cells in mouse spleen cells;\u003c\/p\u003e\n\u003cp\u003e3. The content of tumor antigen-specific T cells in mouse lymph node cells;\u003c\/p\u003e\n\u003cp\u003e4. The content of tumor antigen-specific T cells in mouse tumor tissue cells;\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"border-collapse: collapse; width: 35.5212%; height: 131px;\" border=\"1\" width=\"310.52pt\" cellspacing=\"0\" cellpadding=\"0\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et2\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" width=\"139\" height=\"33\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 32.1992%; text-align: center;\" width=\"138\"\u003e\u003cstrong\u003e20T\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 31.982%; text-align: center;\" width=\"137\"\u003e\u003cstrong\u003e100T\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et3\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" height=\"33\"\u003eNanoparticles\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 32.1992%; text-align: center;\"\u003e20mg\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 31.982%; text-align: center;\"\u003e100mg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et4\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" height=\"33\"\u003ePBS\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 32.1992%; text-align: center;\"\u003e2ml\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 31.982%; text-align: center;\"\u003e10ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. This product is only for in vitro testing, and cannot be edible or injected; Please keep away from the reach of children.\u003cbr\u003e2. Prepare and use it now. Once the nanoparticles are resuspended with culture medium or PBS, immediately add them to the cells to be tested and mix.\u003cbr\u003e3. During use, the fluorescent probes of different antibodies used can be freely matched as needed.\u003cbr\u003e4. Please place the fixed cells at 4 °C and complete the test within one week.\u003cbr\u003e5. This product is only suitable for testing mouse samples.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFreeze-dry storage at-20 ℃ ~-80 ℃, valid for 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100T","offer_id":41683134251083,"sku":null,"price":679.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_1d0b3612-cddb-4515-bf24-5d93778c156b.png?v=1770720273"},{"product_id":"tumor-antigen-specific-t-cell-content-detection-kit-abs57016","title":"4T1-Fluc Breast Cancer Mouse Tumor Antigen-specific T Cell Content Detection Stimulation Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eMethods of use and testing procedures:\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003e1. Add the cells to be detected and the corresponding culture medium to each well of the cell culture dish or cell culture plate, and the cell concentration is 0.8 × 10\u003csup\u003e6\u003c\/sup\u003e-5×10\u003csup\u003e6\u003c\/sup\u003eCells\/mL. Considering the cell loss and the accuracy of flow cytometry detection, it is recommended to use at least 1 million cells. It is recommended to use 12-well or 24-well plate culture, and use at least 1-2mL of culture medium.\u003c\/p\u003e\n\u003cp\u003e2. Add nanoparticles resuspended with PBS or corresponding medium thereto. (The final concentration of nanoparticles used in this test kit is 0.5 mg\/mL-1. 0 mg\/mL)\u003c\/p\u003e\n\u003cp\u003e3. The nanoparticles were incubated with the cells to be detected for 32-48 hours.\u003c\/p\u003e\n\u003cp\u003e4. Add Brefeldin A Solution (BFA) (1000 × diluted into cell culture medium) to the culture medium and incubate for a total of 4 h.\u003c\/p\u003e\n\u003cp\u003e5. Use a pipette to pipette the suspended cells in the collection medium. After collecting the cells, centrifuge the sample cells at 400-500g for 5min. Discard the supernatant and collect the cells.\u003c\/p\u003e\n\u003cp\u003e6. After collecting the cells, resuspend the sample cells in 1 mL PBS.\u003c\/p\u003e\n\u003cp\u003e7. In addition to the blank control, add 2μL of live and dead cell dye to each sample to label live cells and dead cells, mix evenly, and incubate on ice for 15-30 minutes in the dark.\u003c\/p\u003e\n\u003cp\u003e8. After incubation, centrifuge at 400-500g for 5 min, and resuspend the cells with 100μL FACS buffer.\u003c\/p\u003e\n\u003cp\u003e9. Except for the blank control. Add 2 μL Fc Block to each sample to block the Fc receptor on the surface of immune cells, mix well and incubate on ice for 5 min.\u003c\/p\u003e\n\u003cp\u003e10. After incubation, add corresponding membrane-labeled antibodies CD3 antibody, CD4 antibody, and CD8 antibody labeled with different fluorescent dyes to each tube, and incubate on ice for 15-30min.\u003c\/p\u003e\n\u003cp\u003e11. Add 500μL of 4% paraformaldehyde directly to fix the cells and incubate on ice for 40-60min.\u003c\/p\u003e\n\u003cp\u003e12. Centrifuge at 400-500g for 5min, discard the supernatant, then add 100μL of membrane breaking agent to resuspend the cells, and incubate on ice for 30min.\u003c\/p\u003e\n\u003cp\u003e13. After incubation, add the corresponding fluorescent dye-labeled IFN-γ antibody to each tube and incubate on ice for 30 minutes.\u003c\/p\u003e\n\u003cp\u003e14. Centrifuge at 400-500g for 5min, discard the supernatant, resuspend the cells with 200μL FACS buffer or fix the cells with 500μL 4% paraformaldehyde, incubate on ice for 40-60min before detection.\u003c\/p\u003e\n\u003cp\u003e15. Use flow cytometry to detect the proportion of cells that are positive for the labeled antibody. (Note: It is necessary to pass through 400 mesh filter cloth before testing on the machine)\u003c\/p\u003e\n\u003cp\u003e16. Use flow cytometry analysis software to analyze the proportion of CD3 + IFN-γ +, CD3+CD4 + IFN-γ + and CD3+CD8 + IFN-γ + T cells in living cells to the corresponding cells, that is, the proportion and content of tumor antigen-specific T cells (the more cells, the more accurate the detection. It is recommended that the number of living cells collected during detection is more than 100,000, and the number of living cells collected is preferably more than 200,000).\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003eTumor antigen-specific T cells are the main force in killing cancer cells. Due to the high heterogeneity of cancer cells and tumor antigens, cancer cells do not have one or more dominant antigens, so there are few dominant antigen-specific T cells. However, the traditional use of several polypeptide antigens or MHC tetramer technology to detect tumor antigen-specific T cells can only detect antigen-specific T cells against several known specific antigens.\u003c\/p\u003e\n\u003cp\u003eThis product detects the whole cell antigen of cancer cells loaded with nanoparticles. The types of antigens are comprehensive, diverse and broad-spectrum, and can comprehensively and accurately detect tumor antigen-specific T cells in different tissues. Has the advantages of high specificity, high accuracy, comprehensive types of tumor antigen-specific T cells detected, and the like.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTest samples:\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003e1. The content of tumor antigen-specific T cells in peripheral blood immune cells of mice;\u003c\/p\u003e\n\u003cp\u003e2. The content of tumor antigen-specific T cells in mouse spleen cells;\u003c\/p\u003e\n\u003cp\u003e3. The content of tumor antigen-specific T cells in mouse lymph node cells;\u003c\/p\u003e\n\u003cp\u003e4. The content of tumor antigen-specific T cells in mouse tumor tissue cells;\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"border-collapse: collapse; width: 35.5212%; height: 131px;\" border=\"1\" width=\"310.52pt\" cellspacing=\"0\" cellpadding=\"0\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et2\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" width=\"139\" height=\"33\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 32.1992%; text-align: center;\" width=\"138\"\u003e\u003cstrong\u003e20T\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 31.982%; text-align: center;\" width=\"137\"\u003e\u003cstrong\u003e100T\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et3\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" height=\"33\"\u003eNanoparticles\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 32.1992%; text-align: center;\"\u003e20mg\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 31.982%; text-align: center;\"\u003e100mg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et4\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" height=\"33\"\u003ePBS\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 32.1992%; text-align: center;\"\u003e2ml\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 31.982%; text-align: center;\"\u003e10ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. This product is only for in vitro testing, and cannot be edible or injected; Please keep away from the reach of children.\u003cbr\u003e2. Prepare and use it now. Once the nanoparticles are resuspended with culture medium or PBS, immediately add them to the cells to be tested and mix.\u003cbr\u003e3. During use, the fluorescent probes of different antibodies used can be freely matched as needed.\u003cbr\u003e4. Please place the fixed cells at 4 °C and complete the test within one week.\u003cbr\u003e5. This product is only suitable for testing mouse samples.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFreeze-dry storage at-20 ℃ ~-80 ℃, valid for 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"20T","offer_id":41683134283851,"sku":null,"price":171.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":41683134316619,"sku":null,"price":679.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_d51186c9-2c7d-434c-8623-fa50d1ebbc08.png?v=1770720273"},{"product_id":"tumor-antigen-specific-t-cell-content-detection-kit-abs57017","title":"4T1-OVA Breast Cancer Mouse Tumor Antigen-specific T Cell Content Detection Stimulation Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eMethods of use and testing procedures:\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003e1. Add the cells to be detected and the corresponding culture medium to each well of the cell culture dish or cell culture plate, and the cell concentration is 0.8 × 10\u003csup\u003e6\u003c\/sup\u003e-5×10\u003csup\u003e6\u003c\/sup\u003eCells\/mL. Considering the cell loss and the accuracy of flow cytometry detection, it is recommended to use at least 1 million cells. It is recommended to use 12-well or 24-well plate culture, and use at least 1-2mL of culture medium.\u003c\/p\u003e\n\u003cp\u003e2. Add nanoparticles after resuspension with PBS or corresponding medium thereto. (The final concentration of nanoparticles used in this test kit is 0.5 mg\/mL-1. 0 mg\/mL)\u003c\/p\u003e\n\u003cp\u003e3. The nanoparticles were incubated with the cells to be detected for 32-48 hours.\u003c\/p\u003e\n\u003cp\u003e4. Add Brefeldin A Solution (BFA) (1000 × diluted into cell culture medium) to the culture medium and incubate for a total of 4 h.\u003c\/p\u003e\n\u003cp\u003e5. Use a pipette to pipette the suspended cells in the collection medium. After collecting the cells, centrifuge the sample cells at 400-500g for 5min. Discard the supernatant and collect the cells.\u003c\/p\u003e\n\u003cp\u003e6. After collecting the cells, resuspend the sample cells in 1 mL PBS.\u003c\/p\u003e\n\u003cp\u003e7. In addition to the blank control, add 2μL of live and dead cell dye to each sample to label live cells and dead cells, mix evenly, and incubate on ice for 15-30 minutes in the dark.\u003c\/p\u003e\n\u003cp\u003e8. After incubation, centrifuge at 400-500g for 5 min, and resuspend the cells with 100μL FACS buffer.\u003c\/p\u003e\n\u003cp\u003e9. Except for the blank control. Add 2 μL Fc Block to each sample to block the Fc receptor on the surface of immune cells, mix well and incubate on ice for 5 min.\u003c\/p\u003e\n\u003cp\u003e10. After incubation, add corresponding membrane-labeled antibodies CD3 antibody, CD4 antibody, and CD8 antibody labeled with different fluorescent dyes to each tube, and incubate on ice for 15-30min.\u003c\/p\u003e\n\u003cp\u003e11. Add 500μL of 4% paraformaldehyde directly to fix the cells and incubate on ice for 40-60min.\u003c\/p\u003e\n\u003cp\u003e12. Centrifuge at 400-500g for 5min, discard the supernatant, then add 100μL of membrane breaking agent to resuspend the cells, and incubate on ice for 30min.\u003c\/p\u003e\n\u003cp\u003e13. After incubation, add the corresponding fluorescent dye-labeled IFN-γ antibody to each tube and incubate on ice for 30 minutes.\u003c\/p\u003e\n\u003cp\u003e14. Centrifuge at 400-500g for 5min, discard the supernatant, resuspend the cells with 200μL FACS buffer or fix the cells with 500μL 4% paraformaldehyde, incubate on ice for 40-60min before detection.\u003c\/p\u003e\n\u003cp\u003e15. Use flow cytometry to detect the proportion of cells that are positive for the labeled antibody. (Note: It is necessary to pass through 400 mesh filter cloth before testing on the machine)\u003c\/p\u003e\n\u003cp\u003e16. Use flow cytometry analysis software to analyze the proportion of CD3 + IFN-γ +, CD3+CD4 + IFN-γ + and CD3+CD8 + IFN-γ + T cells in living cells to the corresponding cells, that is, the proportion and content of tumor antigen-specific T cells (the more cells, the more accurate the detection. It is recommended that the number of living cells collected during detection is more than 100,000, and the number of living cells collected is preferably more than 200,000).\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003eTumor antigen-specific T cells are the main force in killing cancer cells. Due to the high heterogeneity of cancer cells and tumor antigens, cancer cells do not have one or more dominant antigens, so there are few dominant antigen-specific T cells. However, the traditional use of several polypeptide antigens or MHC tetramer technology to detect tumor antigen-specific T cells can only detect antigen-specific T cells against several known specific antigens.\u003c\/p\u003e\n\u003cp\u003eThis product detects the whole cell antigens of cancer cells loaded with nanoparticles. The types of antigens are comprehensive, diverse and broad-spectrum, and can comprehensively and accurately detect tumor antigen-specific T cells in different tissues. It has the advantages of high specificity, high accuracy, comprehensive types of tumor antigen-specific T cells detected, and the like.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTest samples:\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003e1. The content of tumor antigen-specific T cells in peripheral blood immune cells of mice;\u003c\/p\u003e\n\u003cp\u003e2. The content of tumor antigen-specific T cells in mouse spleen cells;\u003c\/p\u003e\n\u003cp\u003e3. The content of tumor antigen-specific T cells in mouse lymph node cells;\u003c\/p\u003e\n\u003cp\u003e4. The content of tumor antigen-specific T cells in mouse tumor tissue cells;\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"border-collapse: collapse; width: 35.5212%; height: 131px;\" border=\"1\" width=\"310.52pt\" cellspacing=\"0\" cellpadding=\"0\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et2\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" width=\"139\" height=\"33\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 32.1992%; text-align: center;\" width=\"138\"\u003e\u003cstrong\u003e20T\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 31.982%; text-align: center;\" width=\"137\"\u003e\u003cstrong\u003e100T\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et3\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" height=\"33\"\u003eNanoparticles\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 32.1992%; text-align: center;\"\u003e20mg\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 31.982%; text-align: center;\"\u003e100mg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et4\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" height=\"33\"\u003ePBS\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 32.1992%; text-align: center;\"\u003e2ml\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 31.982%; text-align: center;\"\u003e10ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. This product is only for in vitro testing, and cannot be edible or injected; Please keep away from the reach of children.\u003cbr\u003e2. Prepare and use it now. Once the nanoparticles are resuspended with culture medium or PBS, immediately add them to the cells to be tested and mix.\u003cbr\u003e3. During use, the fluorescent probes of different antibodies used can be freely matched as needed.\u003cbr\u003e4. Please place the fixed cells at 4 °C and complete the test within one week.\u003cbr\u003e5. This product is only suitable for testing mouse samples.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFreeze-dry storage at-20 ℃ ~-80 ℃, valid for 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"20T","offer_id":41683134382155,"sku":null,"price":171.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":41683134414923,"sku":null,"price":679.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_c1a20543-0c71-47a2-95ee-fd3b82a6985f.png?v=1770720273"},{"product_id":"tumor-antigen-specific-t-cell-content-detection-kit-abs57018","title":"E0771 Breast Tumor Antigen Specific T Cells in Mice Content Test Stimulus Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eMethods of use and testing procedures:\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003e1. Add the cells to be detected and the corresponding culture medium to each well of the cell culture dish or cell culture plate, and the cell concentration is 0.8 × 10\u003csup\u003e6\u003c\/sup\u003e-5×10\u003csup\u003e6\u003c\/sup\u003eCells\/mL. Considering the cell loss and the accuracy of flow cytometry detection, it is recommended to use at least 1 million cells. It is recommended to use 12-well or 24-well plate culture, and use at least 1-2mL of culture medium.\u003c\/p\u003e\n\u003cp\u003e2. Add nanoparticles after resuspension with PBS or corresponding medium thereto. (The final concentration of nanoparticles used in this test kit is 0.5 mg\/mL-1. 0 mg\/mL)\u003c\/p\u003e\n\u003cp\u003e3. The nanoparticles were incubated with the cells to be detected for 32-48 hours.\u003c\/p\u003e\n\u003cp\u003e4. Add Brefeldin A Solution (BFA) (1000 × diluted into cell culture medium) to the culture medium and incubate for a total of 4 h.\u003c\/p\u003e\n\u003cp\u003e5. Use a pipette to pipette the suspended cells in the collection medium. After collecting the cells, centrifuge the sample cells at 400-500g for 5min. Discard the supernatant and collect the cells.\u003c\/p\u003e\n\u003cp\u003e6. After collecting the cells, resuspend the sample cells in 1 mL PBS.\u003c\/p\u003e\n\u003cp\u003e7. In addition to the blank control, add 2μL of live and dead cell dye to each sample to label live cells and dead cells, mix evenly, and incubate on ice for 15-30 minutes in the dark.\u003c\/p\u003e\n\u003cp\u003e8. After incubation, centrifuge at 400-500g for 5 min, and resuspend the cells with 100μL FACS buffer.\u003c\/p\u003e\n\u003cp\u003e9. Except for the blank control. Add 2 μL Fc Block to each sample to block the Fc receptor on the surface of immune cells, mix well and incubate on ice for 5 min.\u003c\/p\u003e\n\u003cp\u003e10. After incubation, add corresponding membrane-labeled antibodies CD3 antibody, CD4 antibody, and CD8 antibody labeled with different fluorescent dyes to each tube, and incubate on ice for 15-30min.\u003c\/p\u003e\n\u003cp\u003e11. Add 500μL of 4% paraformaldehyde directly to fix the cells and incubate on ice for 40-60min.\u003c\/p\u003e\n\u003cp\u003e12. Centrifuge at 400-500g for 5min, discard the supernatant, then add 100μL of membrane breaking agent to resuspend the cells, and incubate on ice for 30min.\u003c\/p\u003e\n\u003cp\u003e13. After incubation, add the corresponding fluorescent dye-labeled IFN-γ antibody to each tube and incubate on ice for 30 minutes.\u003c\/p\u003e\n\u003cp\u003e14. Centrifuge at 400-500g for 5min, discard the supernatant, resuspend the cells with 200μL FACS buffer or fix the cells with 500μL 4% paraformaldehyde, incubate on ice for 40-60min before detection.\u003c\/p\u003e\n\u003cp\u003e15. Use flow cytometry to detect the proportion of cells that are positive for the labeled antibody. (Note: It is necessary to pass through 400 mesh filter cloth before testing on the machine)\u003c\/p\u003e\n\u003cp\u003e16. Use flow cytometry analysis software to analyze the proportion of CD3 + IFN-γ +, CD3+CD4 + IFN-γ + and CD3+CD8 + IFN-γ + T cells in living cells to the corresponding cells, that is, the proportion and content of tumor antigen-specific T cells (the more cells, the more accurate the detection. It is recommended that the number of living cells collected during detection is more than 100,000, and the number of living cells collected is preferably more than 200,000).\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003eTumor antigen-specific T cells are the main force in killing cancer cells. Due to the high heterogeneity of cancer cells and tumor antigens, cancer cells do not have one or more dominant antigens, so there are few dominant antigen-specific T cells. However, the traditional use of several polypeptide antigens or MHC tetramer technology to detect tumor antigen-specific T cells can only detect antigen-specific T cells against several known specific antigens.\u003c\/p\u003e\n\u003cp\u003eThis product detects the whole cell antigen of cancer cells loaded with nanoparticles. The types of antigens are comprehensive, diverse and broad-spectrum, and can comprehensively and accurately detect tumor antigen-specific T cells in different tissues. Has the advantages of high specificity, high accuracy, comprehensive types of tumor antigen-specific T cells detected, and the like.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTest samples:\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003e1. The content of tumor antigen-specific T cells in peripheral blood immune cells of mice;\u003c\/p\u003e\n\u003cp\u003e2. The content of tumor antigen-specific T cells in mouse spleen cells;\u003c\/p\u003e\n\u003cp\u003e3. The content of tumor antigen-specific T cells in mouse lymph node cells;\u003c\/p\u003e\n\u003cp\u003e4. The content of tumor antigen-specific T cells in mouse tumor tissue cells;\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"border-collapse: collapse; width: 35.5212%; height: 131px;\" border=\"1\" width=\"310.52pt\" cellspacing=\"0\" cellpadding=\"0\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et2\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" width=\"139\" height=\"33\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 32.1992%; text-align: center;\" width=\"138\"\u003e\u003cstrong\u003e20T\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 31.982%; text-align: center;\" width=\"137\"\u003e\u003cstrong\u003e100T\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et3\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" height=\"33\"\u003eNanoparticles\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 32.1992%; text-align: center;\"\u003e20mg\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 31.982%; text-align: center;\"\u003e100mg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et4\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" height=\"33\"\u003ePBS\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 32.1992%; text-align: center;\"\u003e2ml\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 31.982%; text-align: center;\"\u003e10ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. This product is only for in vitro testing, and cannot be edible or injected; Please keep away from the reach of children.\u003cbr\u003e2. Prepare and use it now. Once the nanoparticles are resuspended with culture medium or PBS, immediately add them to the cells to be tested and mix.\u003cbr\u003e3. During use, the fluorescent probes of different antibodies used can be freely matched as needed.\u003cbr\u003e4. Please place the fixed cells at 4 °C and complete the test within one week.\u003cbr\u003e5. This product is only suitable for testing mouse samples.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFreeze-dry storage at-20 ℃ ~-80 ℃, valid for 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"20T","offer_id":41683134447691,"sku":null,"price":171.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":41683134480459,"sku":null,"price":679.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_5d1d3c1c-55f8-4093-a170-97c6fd57edb5.png?v=1770720273"},{"product_id":"tumor-antigen-specific-t-cell-content-detection-kit-abs57019","title":"Hepa 1-6 mouse tumor antigen specific T cell content detection stimulation kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eMethods of use and testing procedures:\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003e1. Add the cells to be detected and the corresponding culture medium to each well of the cell culture dish or cell culture plate, and the cell concentration is 0.8 × 10\u003csup\u003e6\u003c\/sup\u003e-5×10\u003csup\u003e6\u003c\/sup\u003eCells\/mL. Considering the cell loss and the accuracy of flow cytometry detection, it is recommended to use at least 1 million cells. It is recommended to use 12-well or 24-well plate culture, and use at least 1-2mL of culture medium.\u003c\/p\u003e\n\u003cp\u003e2. Add nanoparticles resuspended with PBS or corresponding medium thereto. (The final concentration of nanoparticles used in this test kit is 0.5 mg\/mL-1. 0 mg\/mL)\u003c\/p\u003e\n\u003cp\u003e3. The nanoparticles were incubated with the cells to be detected for 32-48 hours.\u003c\/p\u003e\n\u003cp\u003e4. Add Brefeldin A Solution (BFA) (1000 × diluted into cell culture medium) to the culture medium and incubate for a total of 4 h.\u003c\/p\u003e\n\u003cp\u003e5. Use a pipette to pipette the suspended cells in the collection medium. After collecting the cells, centrifuge the sample cells at 400-500g for 5min. Discard the supernatant and collect the cells.\u003c\/p\u003e\n\u003cp\u003e6. After collecting the cells, resuspend the sample cells in 1 mL PBS.\u003c\/p\u003e\n\u003cp\u003e7. In addition to the blank control, add 2μL of live and dead cell dye to each sample to label live cells and dead cells, mix evenly, and incubate on ice for 15-30 minutes in the dark.\u003c\/p\u003e\n\u003cp\u003e8. After incubation, centrifuge at 400-500g for 5 min, and resuspend the cells with 100μL FACS buffer.\u003c\/p\u003e\n\u003cp\u003e9. Except for the blank control. Add 2 μL Fc Block to each sample to block the Fc receptor on the surface of immune cells, mix well and incubate on ice for 5 min.\u003c\/p\u003e\n\u003cp\u003e10. After incubation, add corresponding membrane-labeled antibodies CD3 antibody, CD4 antibody, and CD8 antibody labeled with different fluorescent dyes to each tube, and incubate on ice for 15-30min.\u003c\/p\u003e\n\u003cp\u003e11. Add 500μL of 4% paraformaldehyde directly to fix the cells and incubate on ice for 40-60min.\u003c\/p\u003e\n\u003cp\u003e12. Centrifuge at 400-500g for 5min, discard the supernatant, then add 100μL of membrane breaking agent to resuspend the cells, and incubate on ice for 30min.\u003c\/p\u003e\n\u003cp\u003e13. After incubation, add the corresponding fluorescent dye-labeled IFN-γ antibody to each tube and incubate on ice for 30 minutes.\u003c\/p\u003e\n\u003cp\u003e14. Centrifuge at 400-500g for 5min, discard the supernatant, resuspend the cells with 200μL FACS buffer or fix the cells with 500μL 4% paraformaldehyde, incubate on ice for 40-60min before detection.\u003c\/p\u003e\n\u003cp\u003e15. Use flow cytometry to detect the proportion of cells that are positive for the labeled antibody. (Note: It is necessary to pass through 400 mesh filter cloth before testing on the machine)\u003c\/p\u003e\n\u003cp\u003e16. Use flow cytometry analysis software to analyze the proportion of CD3 + IFN-γ +, CD3+CD4 + IFN-γ + and CD3+CD8 + IFN-γ + T cells in living cells to the corresponding cells, that is, the proportion and content of tumor antigen-specific T cells (the more cells, the more accurate the detection. It is recommended that the number of living cells collected during detection is more than 100,000, and the number of living cells collected is preferably more than 200,000).\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003eTumor antigen-specific T cells are the main force in killing cancer cells. Due to the high heterogeneity of cancer cells and tumor antigens, cancer cells do not have one or more dominant antigens, so there are few dominant antigen-specific T cells. However, the traditional use of several polypeptide antigens or MHC tetramer technology to detect tumor antigen-specific T cells can only detect antigen-specific T cells against several known specific antigens.\u003c\/p\u003e\n\u003cp\u003eThis product detects the whole cell antigen of cancer cells loaded with nanoparticles. The types of antigens are comprehensive, diverse and broad-spectrum, and can comprehensively and accurately detect tumor antigen-specific T cells in different tissues. Has the advantages of high specificity, high accuracy, comprehensive types of tumor antigen-specific T cells detected, and the like.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTest samples:\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003e1. The content of tumor antigen-specific T cells in peripheral blood immune cells of mice;\u003c\/p\u003e\n\u003cp\u003e2. The content of tumor antigen-specific T cells in mouse spleen cells;\u003c\/p\u003e\n\u003cp\u003e3. The content of tumor antigen-specific T cells in mouse lymph node cells;\u003c\/p\u003e\n\u003cp\u003e4. The content of tumor antigen-specific T cells in mouse tumor tissue cells;\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"border-collapse: collapse; width: 35.5212%; height: 131px;\" border=\"1\" width=\"310.52pt\" cellspacing=\"0\" cellpadding=\"0\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et2\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" width=\"139\" height=\"33\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 32.1992%; text-align: center;\" width=\"138\"\u003e\u003cstrong\u003e20T\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 31.982%; text-align: center;\" width=\"137\"\u003e\u003cstrong\u003e100T\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et3\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" height=\"33\"\u003eNanoparticles\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 32.1992%; text-align: center;\"\u003e20mg\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 31.982%; text-align: center;\"\u003e100mg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et4\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" height=\"33\"\u003ePBS\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 32.1992%; text-align: center;\"\u003e2ml\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 31.982%; text-align: center;\"\u003e10ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. This product is only for in vitro testing, and cannot be edible or injected; Please keep away from the reach of children.\u003cbr\u003e2. Prepare and use it now. Once the nanoparticles are resuspended with culture medium or PBS, immediately add them to the cells to be tested and mix.\u003cbr\u003e3. During use, the fluorescent probes of different antibodies used can be freely matched as needed.\u003cbr\u003e4. Please place the fixed cells at 4 °C and complete the test within one week.\u003cbr\u003e5. This product is only suitable for testing mouse samples.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFreeze-dry storage at-20 ℃ ~-80 ℃, valid for 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"20T","offer_id":41683134513227,"sku":null,"price":171.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":41683134545995,"sku":null,"price":679.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_d6a7cbca-19fd-4769-bcbd-e629b604ad6d.png?v=1770720272"},{"product_id":"tumor-antigen-specific-t-cell-content-detection-kit-abs57020","title":"Hepa 1-6 - OVA mice liver tumor antigen specific T cells content test stimulus kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eMethods of use and testing procedures:\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003e1. Add the cells to be detected and the corresponding culture medium to each well of the cell culture dish or cell culture plate, and the cell concentration is 0.8 × 10\u003csup\u003e6\u003c\/sup\u003e-5×10\u003csup\u003e6\u003c\/sup\u003eCells\/mL. Considering the cell loss and the accuracy of flow cytometry detection, it is recommended to use at least 1 million cells. It is recommended to use 12-well or 24-well plate culture, and use at least 1-2mL of culture medium.\u003c\/p\u003e\n\u003cp\u003e2. Add nanoparticles resuspended with PBS or corresponding medium thereto. (The final concentration of nanoparticles used in this test kit is 0.5 mg\/mL-1. 0 mg\/mL)\u003c\/p\u003e\n\u003cp\u003e3. The nanoparticles were incubated with the cells to be detected for 32-48 hours.\u003c\/p\u003e\n\u003cp\u003e4. Add Brefeldin A Solution (BFA) (1000 × diluted into cell culture medium) to the culture medium and incubate for a total of 4 h.\u003c\/p\u003e\n\u003cp\u003e5. Use a pipette to pipette the suspended cells in the collection medium. After collecting the cells, centrifuge the sample cells at 400-500g for 5min. Discard the supernatant and collect the cells.\u003c\/p\u003e\n\u003cp\u003e6. After collecting the cells, resuspend the sample cells in 1 mL PBS.\u003c\/p\u003e\n\u003cp\u003e7. In addition to the blank control, add 2μL of live and dead cell dye to each sample to label live cells and dead cells, mix evenly, and incubate on ice for 15-30 minutes in the dark.\u003c\/p\u003e\n\u003cp\u003e8. After incubation, centrifuge at 400-500g for 5 min, and resuspend the cells with 100μL FACS buffer.\u003c\/p\u003e\n\u003cp\u003e9. Except for the blank control. Add 2 μL Fc Block to each sample to block the Fc receptor on the surface of immune cells, mix well and incubate on ice for 5 min.\u003c\/p\u003e\n\u003cp\u003e10. After incubation, add corresponding membrane-labeled antibodies CD3 antibody, CD4 antibody, and CD8 antibody labeled with different fluorescent dyes to each tube, and incubate on ice for 15-30min.\u003c\/p\u003e\n\u003cp\u003e11. Add 500μL of 4% paraformaldehyde directly to fix the cells and incubate on ice for 40-60min.\u003c\/p\u003e\n\u003cp\u003e12. Centrifuge at 400-500g for 5min, discard the supernatant, then add 100μL of membrane breaking agent to resuspend the cells, and incubate on ice for 30min.\u003c\/p\u003e\n\u003cp\u003e13. After incubation, add the corresponding fluorescent dye-labeled IFN-γ antibody to each tube and incubate on ice for 30 minutes.\u003c\/p\u003e\n\u003cp\u003e14. Centrifuge at 400-500g for 5min, discard the supernatant, resuspend the cells with 200μL FACS buffer or fix the cells with 500μL 4% paraformaldehyde, incubate on ice for 40-60min before detection.\u003c\/p\u003e\n\u003cp\u003e15. Use flow cytometry to detect the proportion of cells that are positive for the labeled antibody. (Note: It is necessary to pass through 400 mesh filter cloth before testing on the machine)\u003c\/p\u003e\n\u003cp\u003e16. Use flow cytometry analysis software to analyze the proportion of CD3 + IFN-γ +, CD3+CD4 + IFN-γ + and CD3+CD8 + IFN-γ + T cells in living cells to the corresponding cells, that is, the proportion and content of tumor antigen-specific T cells (the more cells, the more accurate the detection. It is recommended that the number of living cells collected during detection is more than 100,000, and the number of living cells collected is preferably more than 200,000).\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003eTumor antigen-specific T cells are the main force in killing cancer cells. Due to the high heterogeneity of cancer cells and tumor antigens, cancer cells do not have one or more dominant antigens, so there are few dominant antigen-specific T cells. However, the traditional use of several polypeptide antigens or MHC tetramer technology to detect tumor antigen-specific T cells can only detect antigen-specific T cells against several known specific antigens.\u003c\/p\u003e\n\u003cp\u003eThis product detects the whole cell antigens of cancer cells loaded with nanoparticles. The types of antigens are comprehensive, diverse and broad-spectrum, and can comprehensively and accurately detect tumor antigen-specific T cells in different tissues. It has the advantages of high specificity, high accuracy, comprehensive types of tumor antigen-specific T cells detected, and the like.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTest samples:\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003e1. The content of tumor antigen-specific T cells in peripheral blood immune cells of mice;\u003c\/p\u003e\n\u003cp\u003e2. The content of tumor antigen-specific T cells in mouse spleen cells;\u003c\/p\u003e\n\u003cp\u003e3. The content of tumor antigen-specific T cells in mouse lymph node cells;\u003c\/p\u003e\n\u003cp\u003e4. The content of tumor antigen-specific T cells in mouse tumor tissue cells;\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"border-collapse: collapse; width: 35.5212%; height: 131px;\" border=\"1\" width=\"310.52pt\" cellspacing=\"0\" cellpadding=\"0\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et2\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" width=\"139\" height=\"33\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 32.1992%; text-align: center;\" width=\"138\"\u003e\u003cstrong\u003e20T\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 31.982%; text-align: center;\" width=\"137\"\u003e\u003cstrong\u003e100T\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et3\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" height=\"33\"\u003eNanoparticles\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 32.1992%; text-align: center;\"\u003e20mg\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 31.982%; text-align: center;\"\u003e100mg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et4\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" height=\"33\"\u003ePBS\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 32.1992%; text-align: center;\"\u003e2ml\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 31.982%; text-align: center;\"\u003e10ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. This product is only for in vitro testing, and cannot be edible or injected; Please keep away from the reach of children.\u003cbr\u003e2. Prepare and use it now. Once the nanoparticles are resuspended with culture medium or PBS, immediately add them to the cells to be tested and mix.\u003cbr\u003e3. During use, the fluorescent probes of different antibodies used can be freely matched as needed.\u003cbr\u003e4. Please place the fixed cells at 4 °C and complete the test within one week.\u003cbr\u003e5. This product is only suitable for testing mouse samples.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFreeze-dry storage at-20 ℃ ~-80 ℃, valid for 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"20T","offer_id":41683134611531,"sku":null,"price":171.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":41683134644299,"sku":null,"price":679.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_870aa570-344d-4c42-9418-490afec640d8.png?v=1770720270"},{"product_id":"tumor-antigen-specific-t-cell-content-detection-kit-abs57021","title":"Hepa 1-6-Fluc mouse tumor antigen-specific T cell content detection stimulation kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eMethods of use and testing procedures:\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003e1. Add the cells to be detected and the corresponding culture medium to each well of the cell culture dish or cell culture plate, and the cell concentration is 0.8 × 10\u003csup\u003e6\u003c\/sup\u003e-5×10\u003csup\u003e6\u003c\/sup\u003eCells\/mL. Considering the cell loss and the accuracy of flow cytometry detection, it is recommended to use at least 1 million cells. It is recommended to use 12-well or 24-well plate culture, and use at least 1-2mL of culture medium.\u003c\/p\u003e\n\u003cp\u003e2. Add nanoparticles resuspended with PBS or corresponding medium thereto. (The final concentration of nanoparticles used in this test kit is 0.5 mg\/mL-1. 0 mg\/mL)\u003c\/p\u003e\n\u003cp\u003e3. The nanoparticles were incubated with the cells to be detected for 32-48 hours.\u003c\/p\u003e\n\u003cp\u003e4. Add Brefeldin A Solution (BFA) (1000 × diluted into cell culture medium) to the culture medium and incubate for a total of 4 h.\u003c\/p\u003e\n\u003cp\u003e5. Use a pipette to blow the suspended cells in the collection medium. After collecting the cells, centrifuge the sample cells at 400-500g for 5 minutes, discard the supernatant and collect the cells.\u003c\/p\u003e\n\u003cp\u003e6. After collecting the cells, resuspend the sample cells in 1 mL PBS.\u003c\/p\u003e\n\u003cp\u003e7. In addition to the blank control, add 2μL of live and dead cell dye to each sample to label live cells and dead cells, mix evenly, and incubate on ice for 15-30 minutes in the dark.\u003c\/p\u003e\n\u003cp\u003e8. After incubation, centrifuge at 400-500g for 5 min, and resuspend the cells with 100μL FACS buffer.\u003c\/p\u003e\n\u003cp\u003e9. Except for the blank control. Add 2 μL Fc Block to each sample to block the Fc receptor on the surface of immune cells, mix well and incubate on ice for 5 min.\u003c\/p\u003e\n\u003cp\u003e10. After incubation, add corresponding membrane-labeled antibodies CD3 antibody, CD4 antibody, and CD8 antibody labeled with different fluorescent dyes to each tube, and incubate on ice for 15-30min.\u003c\/p\u003e\n\u003cp\u003e11. Add 500μL of 4% paraformaldehyde directly to fix the cells and incubate on ice for 40-60min.\u003c\/p\u003e\n\u003cp\u003e12. Centrifuge at 400-500g for 5min, discard the supernatant, then add 100μL of membrane breaking agent to resuspend the cells, and incubate on ice for 30min.\u003c\/p\u003e\n\u003cp\u003e13. After incubation, add the corresponding fluorescent dye-labeled IFN-γ antibody to each tube and incubate on ice for 30 minutes.\u003c\/p\u003e\n\u003cp\u003e14. Centrifuge at 400-500g for 5min, discard the supernatant, resuspend the cells with 200μL FACS buffer or fix the cells with 500μL 4% paraformaldehyde, incubate on ice for 40-60min before detection.\u003c\/p\u003e\n\u003cp\u003e15. Use flow cytometry to detect the proportion of cells that are positive for the labeled antibody. (Note: It is necessary to pass through 400 mesh filter cloth before testing on the machine)\u003c\/p\u003e\n\u003cp\u003e16. Use flow cytometry analysis software to analyze the proportion of CD3 + IFN-γ +, CD3+CD4 + IFN-γ + and CD3+CD8 + IFN-γ + T cells in living cells to the corresponding cells, that is, the proportion and content of tumor antigen-specific T cells (the more cells, the more accurate the detection. It is recommended that the number of living cells collected during detection is more than 100,000, and the number of living cells collected is preferably more than 200,000).\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003eTumor antigen-specific T cells are the main force in killing cancer cells. Due to the high heterogeneity of cancer cells and tumor antigens, cancer cells do not have one or more dominant antigens, so there are few dominant antigen-specific T cells. However, the traditional use of several polypeptide antigens or MHC tetramer technology to detect tumor antigen-specific T cells can only detect antigen-specific T cells against several known specific antigens.\u003c\/p\u003e\n\u003cp\u003eThis product detects the whole cell antigen of cancer cells loaded with nanoparticles. The types of antigens are comprehensive, diverse and broad-spectrum, and can comprehensively and accurately detect tumor antigen-specific T cells in different tissues. It has the advantages of high specificity, high accuracy, comprehensive types of tumor antigen-specific T cells detected, and the like.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTest samples:\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003e1. The content of tumor antigen-specific T cells in peripheral blood immune cells of mice;\u003c\/p\u003e\n\u003cp\u003e2. The content of tumor antigen-specific T cells in mouse spleen cells;\u003c\/p\u003e\n\u003cp\u003e3. The content of tumor antigen-specific T cells in mouse lymph node cells;\u003c\/p\u003e\n\u003cp\u003e4. The content of tumor antigen-specific T cells in mouse tumor tissue cells;\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"border-collapse: collapse; width: 35.5212%; height: 131px;\" border=\"1\" width=\"310.52pt\" cellspacing=\"0\" cellpadding=\"0\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et2\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" width=\"139\" height=\"33\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 32.1992%; text-align: center;\" width=\"138\"\u003e\u003cstrong\u003e20T\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 31.982%; text-align: center;\" width=\"137\"\u003e\u003cstrong\u003e100T\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et3\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" height=\"33\"\u003eNanoparticles\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 32.1992%; text-align: center;\"\u003e20mg\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 31.982%; text-align: center;\"\u003e100mg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et4\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" height=\"33\"\u003ePBS\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 32.1992%; text-align: center;\"\u003e2ml\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 31.982%; text-align: center;\"\u003e10ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. This product is only for in vitro testing, and cannot be edible or injected; Please keep away from the reach of children.\u003cbr\u003e2. Prepare and use it now. Once the nanoparticles are resuspended with culture medium or PBS, immediately add them to the cells to be tested and mix.\u003cbr\u003e3. During use, the fluorescent probes of different antibodies used can be freely matched as needed.\u003cbr\u003e4. Please place the fixed cells at 4 °C and complete the test within one week.\u003cbr\u003e5. This product is only suitable for testing mouse samples.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFreeze-dry storage at-20 ℃ ~-80 ℃, valid for 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"20T","offer_id":41683134677067,"sku":null,"price":171.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":41683134709835,"sku":null,"price":679.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_cb1b7d1d-c878-44b5-a3ca-71864e7c7064.png?v=1770720270"},{"product_id":"tumor-antigen-specific-t-cell-content-detection-kit-abs57022","title":"AKR Esophageal Cancer Mouse Tumor Antigen-specific T Cell Content Detection Stimulation Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eMethods of use and testing procedures:\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003e1. Add the cells to be detected and the corresponding culture medium to each well of the cell culture dish or cell culture plate, and the cell concentration is 0.8 × 10\u003csup\u003e6\u003c\/sup\u003e-5×10\u003csup\u003e6\u003c\/sup\u003eCells\/mL. Considering the cell loss and the accuracy of flow cytometry detection, it is recommended to use at least 1 million cells. It is recommended to use 12-well or 24-well plate culture, and use at least 1-2mL of culture medium.\u003c\/p\u003e\n\u003cp\u003e2. Add nanoparticles resuspended with PBS or corresponding medium thereto. (The final concentration of nanoparticles used in this test kit is 0.5 mg\/mL-1. 0 mg\/mL)\u003c\/p\u003e\n\u003cp\u003e3. The nanoparticles were incubated with the cells to be detected for 32-48 hours.\u003c\/p\u003e\n\u003cp\u003e4. Add Brefeldin A Solution (BFA) (1000 × diluted into cell culture medium) to the culture medium and incubate for a total of 4 h.\u003c\/p\u003e\n\u003cp\u003e5. Use a pipette to pipette the suspended cells in the collection medium. After collecting the cells, centrifuge the sample cells at 400-500g for 5min. Discard the supernatant and collect the cells.\u003c\/p\u003e\n\u003cp\u003e6. After collecting the cells, resuspend the sample cells in 1 mL PBS.\u003c\/p\u003e\n\u003cp\u003e7. In addition to the blank control, add 2μL of live and dead cell dye to each sample to label live cells and dead cells, mix evenly, and incubate on ice for 15-30 minutes in the dark.\u003c\/p\u003e\n\u003cp\u003e8. After incubation, centrifuge at 400-500g for 5 min, and resuspend the cells with 100μL FACS buffer.\u003c\/p\u003e\n\u003cp\u003e9. Except for the blank control. Add 2 μL Fc Block to each sample to block the Fc receptor on the surface of immune cells, mix well and incubate on ice for 5 min.\u003c\/p\u003e\n\u003cp\u003e10. After incubation, add corresponding membrane-labeled antibodies CD3 antibody, CD4 antibody, and CD8 antibody labeled with different fluorescent dyes to each tube, and incubate on ice for 15-30min.\u003c\/p\u003e\n\u003cp\u003e11. Add 500μL of 4% paraformaldehyde directly to fix the cells and incubate on ice for 40-60min.\u003c\/p\u003e\n\u003cp\u003e12. Centrifuge at 400-500g for 5min, discard the supernatant, then add 100μL of membrane breaking agent to resuspend the cells, and incubate on ice for 30min.\u003c\/p\u003e\n\u003cp\u003e13. After incubation, add the corresponding fluorescent dye-labeled IFN-γ antibody to each tube and incubate on ice for 30 minutes.\u003c\/p\u003e\n\u003cp\u003e14. Centrifuge at 400-500g for 5min, discard the supernatant, resuspend the cells with 200μL FACS buffer or fix the cells with 500μL 4% paraformaldehyde, incubate on ice for 40-60min before detection.\u003c\/p\u003e\n\u003cp\u003e15. Use flow cytometry to detect the proportion of cells that are positive for the labeled antibody. (Note: It is necessary to pass through 400 mesh filter cloth before testing on the machine)\u003c\/p\u003e\n\u003cp\u003e16. Use flow cytometry analysis software to analyze the proportion of CD3 + IFN-γ +, CD3+CD4 + IFN-γ + and CD3+CD8 + IFN-γ + T cells in living cells to the corresponding cells, that is, the proportion and content of tumor antigen-specific T cells (the more cells, the more accurate the detection. It is recommended that the number of living cells collected during detection is more than 100,000, and the number of living cells collected is preferably more than 200,000).\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003eTumor antigen-specific T cells are the main force in killing cancer cells. Due to the high heterogeneity of cancer cells and tumor antigens, cancer cells do not have one or more dominant antigens, so there are few dominant antigen-specific T cells. However, the traditional use of several polypeptide antigens or MHC tetramer technology to detect tumor antigen-specific T cells can only detect antigen-specific T cells against several known specific antigens.\u003c\/p\u003e\n\u003cp\u003eThis product detects the whole cell antigens of cancer cells loaded with nanoparticles. The types of antigens are comprehensive, diverse and broad-spectrum, and can comprehensively and accurately detect tumor antigen-specific T cells in different tissues. It has the advantages of high specificity, high accuracy, comprehensive types of tumor antigen-specific T cells detected, and the like.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTest samples:\u003c\/strong\u003e \u003c\/p\u003e\n\u003cp\u003e1. The content of tumor antigen-specific T cells in peripheral blood immune cells of mice;\u003c\/p\u003e\n\u003cp\u003e2. The content of tumor antigen-specific T cells in mouse spleen cells;\u003c\/p\u003e\n\u003cp\u003e3. The content of tumor antigen-specific T cells in mouse lymph node cells;\u003c\/p\u003e\n\u003cp\u003e4. The content of tumor antigen-specific T cells in mouse tumor tissue cells;\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"border-collapse: collapse; width: 35.5212%; height: 131px;\" border=\"1\" width=\"310.52pt\" cellspacing=\"0\" cellpadding=\"0\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et2\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" width=\"139\" height=\"33\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 32.1992%; text-align: center;\" width=\"138\"\u003e\u003cstrong\u003e20T\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 31.982%; text-align: center;\" width=\"137\"\u003e\u003cstrong\u003e100T\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et3\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" height=\"33\"\u003eNanoparticles\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 32.1992%; text-align: center;\"\u003e20mg\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 31.982%; text-align: center;\"\u003e100mg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 25.00pt;\"\u003e\n\u003ctd class=\"et4\" style=\"height: 25pt; width: 32.4179%; text-align: center;\" height=\"33\"\u003ePBS\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 32.1992%; text-align: center;\"\u003e2ml\u003c\/td\u003e\n\u003ctd class=\"et4\" style=\"width: 31.982%; text-align: center;\"\u003e10ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. This product is only for in vitro testing, and cannot be edible or injected; Please keep away from the reach of children.\u003cbr\u003e2. Prepare and use it now. Once the nanoparticles are resuspended with culture medium or PBS, immediately add them to the cells to be tested and mix.\u003cbr\u003e3. During use, the fluorescent probes of different antibodies used can be freely matched as needed.\u003cbr\u003e4. Please place the fixed cells at 4 °C and complete the test within one week.\u003cbr\u003e5. This product is only suitable for testing mouse samples.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFreeze-dry storage at-20 ℃ ~-80 ℃, valid for 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"20T","offer_id":41683134742603,"sku":null,"price":171.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":41683134775371,"sku":null,"price":679.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_e76ca7a1-1a20-4da0-85cd-4d4ee3f0f52f.png?v=1770720270"},{"product_id":"tumor-antigen-specific-t-cell-content-detection-kit-abs57023","title":"Human tumor antigen-specific T cell content detection stimulation kit for non-small cell lung cancer","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eMethods of use and testing procedures:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. Add the cells to be detected and AIM V serum-free medium to each well of the cell culture dish or cell culture plate, and the cell concentration is 0.8 × 10\u003csup\u003e6\u003c\/sup\u003e-2×10\u003csup\u003e6\u003c\/sup\u003eCells\/mL. Considering the cell loss and the accuracy of flow cytometry detection, it is recommended to use at least 1 million cells. It is recommended to use 12-well plate or 24-well plate culture, and use at least 1-2mL of culture medium.\u003c\/p\u003e\n\u003cp\u003e2. Add nanoparticles resuspended with PBS or corresponding medium to it. The final concentration of nanoparticles detected in this detection kit is 0.5 mg\/mL-1. 0 mg\/mL.\u003c\/p\u003e\n\u003cp\u003e3. The nanoparticles were incubated with the cells to be detected for 32-48 hours. It is recommended that at least two replicates of the sample to be tested be set; Negative and positive controls need to be set up.\u003cstrong\u003eNote:\u003c\/strong\u003eThe final concentration of nanoparticles when co-incubated with cells was 0.5 mg\/mL-1. 0 mg\/mL; Cells not cultured with nano particles at the time of detection were used as negative controls; Cells cultured after adding T cell stimulators such as LPS (generally stimulated with LPS for 8-16 hours) are used as positive controls.\u003c\/p\u003e\n\u003cp\u003e4. Add Brefeldin A Solution (BFA) (1000 × diluted into cell culture medium) to the culture medium and incubate for a total of 4 h.\u003c\/p\u003e\n\u003cp\u003e5. Use a pipette to pipette the suspended cells in the collection medium. After collecting the cells, centrifuge the sample cells at 400-500g for 5min. Discard the supernatant and collect the cells.\u003c\/p\u003e\n\u003cp\u003e6. After collecting the cells, resuspend the sample cells in 1 mL PBS.\u003c\/p\u003e\n\u003cp\u003e7. In addition to the blank control, add 2μL of live and dead cell dye to each sample to label live cells and dead cells, mix evenly, and incubate on ice for 15-30 minutes in the dark.\u003c\/p\u003e\n\u003cp\u003e8. After incubation, centrifuge at 400-500g for 5 min, and resuspend the cells with 100μL FACS buffer.\u003c\/p\u003e\n\u003cp\u003e9. Except for the blank control. Add 2 μL Fc Block to each sample to block the Fc receptor on the surface of immune cells, mix well and incubate on ice for 5 min.\u003c\/p\u003e\n\u003cp\u003e10. After incubation, add corresponding membrane-labeled antibodies (CD3, CD4, CD8 antibodies) labeled with different fluorescent dyes to each tube, and incubate on ice for 15-30 minutes.\u003c\/p\u003e\n\u003cp\u003e11. Add 500μL of 4% paraformaldehyde directly to fix the cells and incubate on ice for 40-60 minutes.\u003c\/p\u003e\n\u003cp\u003e12. Centrifuge at 400-500g for 5min, discard the supernatant, then add 100μL of membrane breaking agent to resuspend the cells, and incubate on ice for 30min.\u003c\/p\u003e\n\u003cp\u003e13. After incubation, add the corresponding fluorescent dye-labeled IFN-γ antibody and\/or granzyme B (GzmB) antibody to each tube, and incubate on ice for 30 minutes.\u003c\/p\u003e\n\u003cp\u003e14. Centrifuge at 400-500g for 5min, discard the supernatant, resuspend the cells with 200μL FACS buffer or fix the cells with 500μL 4% paraformaldehyde, incubate on ice for 40-60min before detection.\u003c\/p\u003e\n\u003cp\u003e15. Flow cytometry is used to detect the proportion of cells positive for the labeled antibody. (Note: Test after the 400-mesh filter cloth is tested on the machine).\u003c\/p\u003e\n\u003cp\u003e16. Use flow cytometry analysis software to analyze the proportion of CD3 + IFN-γ +, CD3+CD4 + IFN-γ + and CD3+CD8 + IFN-γ + T cells in living cells to the corresponding cells, that is, the proportion and content of tumor antigen-specific T cells (the more cells, the more accurate the detection. It is recommended that the number of living cells collected during detection is more than 100,000, and the number of living cells collected is preferably more than 200,000).\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eTumor antigen-specific T cells are the main force in killing cancer cells. Due to the high heterogeneity of cancer cells and tumor antigens, cancer cells do not have one or more dominant antigens, so there are few dominant antigen-specific T cells. However, the traditional use of several polypeptide antigens or MHC tetramer technology to detect tumor antigen-specific T cells can only detect antigen-specific T cells against several known specific antigens.\u003c\/p\u003e\n\u003cp\u003eThis product detects the whole cell antigen of cancer cells loaded with nanoparticles. The types of antigens are comprehensive, diverse and broad-spectrum, and can comprehensively and accurately detect tumor antigen-specific T cells in different tissues. It has the advantages of high specificity, high accuracy, comprehensive types of tumor antigen-specific T cells detected, and the like.\u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTest samples:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. The content of tumor antigen-specific T cells in peripheral blood immune cells (please use heparin sodium as blood anticoagulant, do not use EDTA for anticoagulation, 5-7mL peripheral blood is required);\u003c\/p\u003e\n\u003cp\u003e2. The content of tumor antigen-specific T cells in splenocytes;\u003c\/p\u003e\n\u003cp\u003e3. The content of tumor antigen-specific T cells in lymph node cells;\u003c\/p\u003e\n\u003cp\u003e4. The content of tumor antigen-specific T cells in tumor tissue cells;\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"border-collapse: collapse;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"96\"\u003e\u003cp\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"95\"\u003e\u003cp\u003e\u003cstrong\u003e20T\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"95\"\u003e\u003cp\u003e\u003cstrong\u003e100T\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"96\"\u003e\u003cp\u003eNanoparticles\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"95\"\u003e\u003cp\u003e20mg\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"95\"\u003e\u003cp\u003e100mg\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"96\"\u003e\u003cp\u003ePBS\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"95\"\u003e\u003cp\u003e2ml\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"95\"\u003e\u003cp\u003e10ml\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1. This product is only for in vitro testing, and cannot be edible or injected; Please keep away from the reach of children.\u003c\/p\u003e\n\u003cp\u003e2. Ready to prepare and use, once the nanoparticles are resuspended with AIM V serum-free medium or PBS, immediately add them to the cells to be tested and mix.\u003c\/p\u003e\n\u003cp\u003e3. During use, the fluorescent probes of different antibodies used can be freely matched as needed.\u003c\/p\u003e\n\u003cp\u003e4. Please place the fixed cells at 4 °C and complete the test within one week.\u003cbr\u003e5. This product is only suitable for testing human samples.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis product is easy to absorb moisture, frozen at-20 ℃ ~-80 ℃, sealed and dry, and has a validity period of 1 year.\u003cp\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"20T","offer_id":41683134808139,"sku":null,"price":524.0,"currency_code":"USD","in_stock":true},{"title":"100T","offer_id":41683134840907,"sku":null,"price":1746.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_01d487f2-bc95-442e-b525-5ee9e384854c.png?v=1770720272"},{"product_id":"tumor-antigen-specific-t-cell-content-detection-kit-abs57024","title":"Human tumor antigen-specific T cell content detection stimulation kit for esophageal cancer","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eMethods of use and testing procedures:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. Add the cells to be detected and AIM V serum-free medium to each well of the cell culture dish or cell culture plate, and the cell concentration is 0.8 × 10\u003csup\u003e6\u003c\/sup\u003e-2×10\u003csup\u003e6\u003c\/sup\u003eCells\/mL. Considering the cell loss and the accuracy of flow cytometry detection, it is recommended to use at least 1 million cells. It is recommended to use 12-well plate or 24-well plate culture, and use at least 1-2mL of culture medium.\u003c\/p\u003e\n\u003cp\u003e2. Add nanoparticles resuspended with PBS or corresponding medium to it. The final concentration of nanoparticles detected in this detection kit is 0.5 mg\/mL-1. 0 mg\/mL.\u003c\/p\u003e\n\u003cp\u003e3. The nanoparticles were incubated with the cells to be detected for 32-48 hours. It is recommended that at least two replicates of the sample to be tested be set; Negative and positive controls need to be set up.\u003cstrong\u003eNote:\u003c\/strong\u003eThe final concentration of nanoparticles when co-incubated with cells was 0.5 mg\/mL-1. 0 mg\/mL; Cells not cultured with nano particles at the time of detection were used as negative controls; Cells cultured after adding T cell stimulators such as LPS (generally stimulated with LPS for 8-16 hours) are used as positive controls.\u003c\/p\u003e\n\u003cp\u003e4. Add Brefeldin A Solution (BFA) (1000 × diluted into cell culture medium) to the culture medium and incubate for a total of 4 h.\u003c\/p\u003e\n\u003cp\u003e5. Use a pipette to pipette the suspended cells in the collection medium. After collecting the cells, centrifuge the sample cells at 400-500g for 5min. Discard the supernatant and collect the cells.\u003c\/p\u003e\n\u003cp\u003e6. After collecting the cells, resuspend the sample cells in 1 mL PBS.\u003c\/p\u003e\n\u003cp\u003e7. In addition to the blank control, add 2μL of live and dead cell dye to each sample to label live cells and dead cells, mix evenly, and incubate on ice for 15-30 minutes in the dark.\u003c\/p\u003e\n\u003cp\u003e8. After incubation, centrifuge at 400-500g for 5 min, and resuspend the cells with 100μL FACS buffer.\u003c\/p\u003e\n\u003cp\u003e9. Except for the blank control. Add 2 μL Fc Block to each sample to block the Fc receptor on the surface of immune cells, mix well and incubate on ice for 5 min.\u003c\/p\u003e\n\u003cp\u003e10. After incubation, add corresponding membrane-labeled antibodies (CD3, CD4, CD8 antibodies) labeled with different fluorescent dyes to each tube, and incubate on ice for 15-30 minutes.\u003c\/p\u003e\n\u003cp\u003e11. Add 500μL of 4% paraformaldehyde directly to fix the cells and incubate on ice for 40-60 minutes.\u003c\/p\u003e\n\u003cp\u003e12. Centrifuge at 400-500g for 5min, discard the supernatant, then add 100μL of membrane breaking agent to resuspend the cells, and incubate on ice for 30min.\u003c\/p\u003e\n\u003cp\u003e13. After incubation, add the corresponding fluorescent dye-labeled IFN-γ antibody and\/or granzyme B (GzmB) antibody to each tube, and incubate on ice for 30 minutes.\u003c\/p\u003e\n\u003cp\u003e14. Centrifuge at 400-500g for 5min, discard the supernatant, resuspend the cells with 200μL FACS buffer or fix the cells with 500μL 4% paraformaldehyde, incubate on ice for 40-60min before detection.\u003c\/p\u003e\n\u003cp\u003e15. Flow cytometry is used to detect the proportion of cells positive for the labeled antibody. (Note: Test after the 400-mesh filter cloth is tested on the machine).\u003c\/p\u003e\n\u003cp\u003e16. Use flow cytometry analysis software to analyze the proportion of CD3 + IFN-γ +, CD3+CD4 + IFN-γ + and CD3+CD8 + IFN-γ + T cells in living cells to the corresponding cells, that is, the proportion and content of tumor antigen-specific T cells (the more cells, the more accurate the detection. It is recommended that the number of living cells collected during detection is more than 100,000, and the number of living cells collected is preferably more than 200,000).\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eTumor antigen-specific T cells are the main force in killing cancer cells. Due to the high heterogeneity of cancer cells and tumor antigens, cancer cells do not have one or more dominant antigens, so there are few dominant antigen-specific T cells. However, the traditional use of several polypeptide antigens or MHC tetramer technology to detect tumor antigen-specific T cells can only detect antigen-specific T cells against several known specific antigens.\u003c\/p\u003e\n\u003cp\u003eThis product detects the whole cell antigens of cancer cells loaded with nanoparticles. The types of antigens are comprehensive, diverse and broad-spectrum, and can comprehensively and accurately detect tumor antigen-specific T cells in different tissues. It has the advantages of high specificity, high accuracy, comprehensive types of tumor antigen-specific T cells detected, and the like.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTest samples:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. The content of tumor antigen-specific T cells in peripheral blood immune cells (please use heparin sodium as blood anticoagulant, do not use EDTA for anticoagulation, 5-7mL peripheral blood is required);\u003c\/p\u003e\n\u003cp\u003e2. The content of tumor antigen-specific T cells in splenocytes;\u003c\/p\u003e\n\u003cp\u003e3. The content of tumor antigen-specific T cells in lymph node cells;\u003c\/p\u003e\n\u003cp\u003e4. The content of tumor antigen-specific T cells in tumor tissue cells;\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"border-collapse: collapse;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"96\"\u003e\u003cp\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"95\"\u003e\u003cp\u003e\u003cstrong\u003e20T\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"95\"\u003e\u003cp\u003e\u003cstrong\u003e100T\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"96\"\u003e\u003cp\u003eNanoparticles\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"95\"\u003e\u003cp\u003e20mg\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"95\"\u003e\u003cp\u003e100mg\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"96\"\u003e\u003cp\u003ePBS\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"95\"\u003e\u003cp\u003e2ml\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd width=\"95\"\u003e\u003cp\u003e10ml\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1. This product is only for in vitro testing, and cannot be edible or injected; Please keep away from the reach of children.\u003c\/p\u003e\n\u003cp\u003e2. Ready to prepare and use, once the nanoparticles are resuspended with AIM V serum-free medium or PBS, immediately add them to the cells to be tested and mix.\u003c\/p\u003e\n\u003cp\u003e3. During use, the fluorescent probes of different antibodies used can be freely matched as needed.\u003c\/p\u003e\n\u003cp\u003e4. Please place the fixed cells at 4 °C and complete the test within one week.\u003cbr\u003e5. This product is only suitable for testing human samples.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eThis product is easy to absorb moisture, frozen at-20 ℃ ~-80 ℃, sealed and dry, and has a validity period of 1 year.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"100T","offer_id":41683134939211,"sku":null,"price":1746.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_49ada5a4-244d-4428-812e-784ad4e6c5a4.png?v=1770720271"},{"product_id":"mouse-nave-cd4-t-cell-isolation-kit-column-free-s0k0014","title":"Mouse Naïve CD4 T Cell Isolation Kit (Column-Free)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e1 mL Mouse Naïve CD4 T Cell Biotin-Antibody Cocktail\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1mL Streptavidin Beads\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eSuitable for a total cell count of 1×10⁹, and can perform up to 100 sorting runs.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eIn this negative selection process, non-target cells are labeled with biotin-conjugated antibodies, and the cells are then incubated with streptavidin-nanomagnetic beads. The non-target cells are marked by the antibody‑bead complex, while the target\u003c\/span\u003e\u003cspan style=\"color:#404040\"\u003eNaïve CD4 T\u003c\/span\u003e\u003cspan\u003ecells remain unlabeled. Subsequently, the cell suspension is transferred to a polystyrene tube and placed within the sorter's magnetic field. Under the influence of the magnetic field, the magnetically labeled non-target cells adhere to the tube wall, whereas the unlabeled\u003c\/span\u003e\u003cspan style=\"color:#404040\"\u003eNaïve CD4 T\u003c\/span\u003e\u003cspan\u003ecells remain in the supernatant. This supernatant constitutes the\u003c\/span\u003e\u003cspan style=\"color:#404040\"\u003eNaïve CD4 T\u003c\/span\u003e\u003cspan\u003ecell-enriched fraction after removal of the non-target cells.\u003c\/span\u003e\u003cspan style=\"color:#404040\"\u003eNaïve CD4 T\u003c\/span\u003e\u003cspan\u003ecell fraction.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eMouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e150 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eNegative Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8 °C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"color:#000000\"\u003eMouse naïve CD4 T cells refer to mature CD4 T cells that have never encountered a specific antigen and remain in a quiescent state. They serve as the common precursor for all effector CD4 T cell subsets (such as Th1\/Th2\/Th17\/Treg) and are a model system for studying the mechanisms of T cell activation, differentiation, and function. Using a column-free Mouse Naïve CD4 T cell isolation kit, antibody-naïve, initial CD4 T cells can be rapidly and efficiently purified. This method removes only non-target cells during the sorting process without labeling or activating the target naïve CD4 T cells, thereby preserving their native phenotype and functional integrity, making them suitable for subsequent cell culture and functional assays.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cspan style=\"display:inline; white-space:nowrap;\"\u003eNote: This kit does not include sorting buffer; please prepare it yourself. The corresponding Stater catalog number is\u003ca href=\"https:\/\/www.starter-bio.com\/s0d3018_starter_magsep_separation_buffer.html\" style=\"color:#0066FF; display:inline; white-space:nowrap;\"\u003eS0D3018\u003c\/a\u003e\u003c\/span\u003e\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eSteps\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eOperating Instructions\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eDosage and Time\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCollect the mouse spleen, grind it on a 40 μm sterile filter to prepare a single-cell suspension of splenic cells; after red blood cell lysis, proceed with cell counting.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eIt is recommended to count cells after staining with AO\/PI; splenic cells can be centrifuged at 400 g for 7 minutes.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eSample Preparation\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eResuspend 1×10 in 100 μL of MagSep Separation Buffer\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ecells;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eAdjust reagents proportionally according to sample volume; retain a pre-sorting sample to measure the Naïve CD4 ratio.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ecells\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 10 μL of Mouse Naïve CD4 T cell Biotin Antibody Cocktail to the sample and gently mix.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eFor this step, gently pipette up and down 2–3 times to mix thoroughly;\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10 μL \/ 100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate the antibodies in the cocktail with the cells at room temperature for 10 minutes;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate at room temperature for 10 minutes\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eShake for 5–30 seconds to mix the Streptavidin beads evenly;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eMix for 5–30 seconds\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e6\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 10 μL of Streptavidin beads to the sample;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10 μL\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eGently mix the magnetic beads and cells, and incubate at room temperature for 5 minutes;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eFor this step, you can use a pipette to gently blow the mixture 2–3 times to mix it evenly;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDuring this step, the magnetic beads may settle at the bottom; you can lightly tap the tube to remix them around the 2.5-minute mark;\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate at room temperature for 5 minutes\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd MagSep Separation Buffer to the sample, and immediately place it on the magnetic stand to prevent the beads from settling heavily at the bottom;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd the total volume to 2.5 mL (in a 5 mL flow cytometry tube)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e9\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003ePlace the sample on a single-well magnetic stand so that the magnetic beads adhere to the tube wall;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eHold the sample on the single-well magnetic stand for 10 minutes\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTilt the magnetic stand and pour the sample into a new collection tube to collect it;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eNote: The sorted cells can be centrifuged at 400g for 7 minutes for subsequent culture and analysis.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCell sorting successful\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003e0.1-1 × 10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ecells (0.1–1 mL) are recommended to use a 5 mL single-well magnetic separator (Starter EasyIso Separator) to adsorb a total volume of 2.5 mL;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"100T","offer_id":42302133895243,"sku":"S0K0014-100T","price":800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/ad294d675ab8423e8b15a71184e3da63.jpg?v=1788184811"},{"product_id":"293t-cell-lysate-s0y0002","title":"293T Cell Lysate","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eWB\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e2 mg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eSupplied in SDS Sample Buffer: 50 mM Tris-HCl (pH 6.8 at 25°C), 2% w\/v SDS, 10% glycerol, 1% v\/v β-Mercaptoethanol, 0.01% w\/v bromophenol blue. \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-20 °C for 1 months under sterile conditions; \u003cbr\u003e-80 °C for 24 months under sterile conditions.\u003cbr\u003ePlease avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"100μg","offer_id":42332847669323,"sku":"S0Y0002-100μg","price":135.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_68a8a60c-b404-4bf8-8182-915a80378574.png?v=1788523424"},{"product_id":"jurkat-cell-lysate-s0y0009","title":"Jurkat cell lysate","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e2 mg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eSupplied in SDS Sample Buffer: 50 mM Tris-HCl (pH 6.8 at 25°C), 2% w\/v SDS, 10% glycerol, 1% v\/v β-Mercaptoethanol, 0.01% w\/v bromophenol blue\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-20 °C for 1 months under sterile conditions; \u003cbr\u003e-80 °C for 24 months under sterile conditions.\u003cbr\u003ePlease avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"100μg","offer_id":42332847734859,"sku":"S0Y0009-100μg","price":170.0,"currency_code":"USD","in_stock":true},{"title":"1mg","offer_id":43091409502283,"sku":"S0Y0009-1mg","price":750.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_10f9fc44-f666-423c-a47a-262e949a4f81.png?v=1788523438"},{"product_id":"human-nave-cd4-t-cell-isolation-kit-column-free-s0k3014","title":"Human Naïve CD4 T Cell Isolation Kit (Column-Free)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e1 mL Human Naïve CD4 T Cell Biotin-Antibody Cocktail, PBS buffer containing BSA;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1.5 mL Streptavidin Beads, PBS buffer containing BSA and Poloxamer 188;\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eSuitable for a total cell count of 1×10⁹, and can perform up to 100 sorting runs.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eIn this negative selection process, non-human naïve CD4 T cells are labeled with biotin-conjugated antibodies, and the cells are then incubated with streptavidin-nanomagnetic beads. As a result, the non-target cells are marked by the antibody‑bead complex, while the target human naïve CD4 T cells remain unlabeled. Subsequently, the cell suspension is transferred to a polystyrene tube and placed within the magnetic field of the sorter. Under the influence of the magnetic field, the magnetically labeled non-target cells adhere to the tube wall, whereas the unlabeled human naïve CD4 T cells remain in the supernatant. This supernatant constitutes the enriched fraction of human naïve CD4 T cells after removal of the non-target cells.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eApplications in cell sorting\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eNaïve CD4 T cells in their unmanipulated, label-free state are isolated from human PBMCs and can be used for a variety of downstream functional assays.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e150 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eNegative Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8 °C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan\u003eThe immunophenotype of human naïve CD4 T cells is CD3⁺CD4⁺CD45RA⁺CD45RO⁻. They constitute the core precursor population of helper T cells in the human adaptive immune system, responsible for initial antigen recognition and directed differentiation, and are also a key cell subset for maintaining immune homeostasis, mediating specific responses, and establishing immunological memory. The human naïve CD4 T-cell negative-selection kit is developed based on immuno‑magnetic bead–mediated negative selection, overcoming the labeling limitations inherent in positive selection. By specifically depleting non‑target cells—such as CD8 T cells, NK cells, B cells, monocytes, dendritic cells, granulocytes, and memory T cells—it yields unlabeled, native‑state, highly purified naïve CD4 T cells, perfectly preserving their initial quiescent state, surface molecule integrity, and multipotential differentiation capacity. This makes them well suited for various functional assays, including in vitro activation and proliferation, subtype‑directed differentiation induction, and cytokine secretion analysis, and they have become the mainstream research tool for isolating human naïve CD4 T cells.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cspan style=\"display:inline; white-space:nowrap;\"\u003eNote: This kit does not include the sorting buffer; you will need to provide it yourself. The corresponding Stater product number is\u003ca href=\"https:\/\/www.starter-bio.com\/s0d3018_starter_magsep_separation_buffer.html\" style=\"color:#0066FF; display:inline; white-space:nowrap;\"\u003eS0D3018\u003c\/a\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eSteps\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eOperating Instructions\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eDosage and Time\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIsolate PBMCs from peripheral blood using density gradient centrifugation with lymphocyte separation solution, or directly thaw cryopreserved PBMC cells, and proceed with cell counting;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eIt is recommended to count cells after staining with AO\/PI; PBMCs can be centrifuged at 400 g for 7 minutes;\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eSample Preparation\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eResuspend 1×10^7 cells in 100 μL of MagSep Separation Buffer.\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ecells;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eAdjust reagents proportionally according to sample volume; set aside a pre-sorting sample to determine the proportion of Human Naïve CD4 T cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1×10^7\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ecells\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 10 μL of Human Naïve CD4 T Cell Biotin Antibody Cocktail to the sample and gently mix.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eIn this step, gently pipette up and down 2–3 times to mix thoroughly;\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10 μL \/ 100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate the antibodies in the cocktail with the cells at room temperature for 10 minutes;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate at room temperature for 10 minutes\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eShake for 5–30 seconds to mix the Streptavidin beads evenly;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eMix for 5–30 seconds\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e6\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 15 μL of Streptavidin beads to the sample;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e15 μL\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eGently mix the magnetic beads and cells, and incubate at room temperature for 8 minutes;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eFor mixing in this step, you can use a pipette to gently blow the mixture 2–3 times;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDuring this step, the magnetic beads may settle at the bottom; you can remix them again after about 2.5 minutes;\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate at room temperature for 8 minutes\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd MagSep Separation Buffer to the sample and immediately place it on the magnetic stand to prevent the magnetic beads from settling excessively;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd the total volume to 2.5 mL (in a 5 mL flow cytometry tube)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd the total volume to 7.5 mL (in a 15 mL centrifuge tube)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e9\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003ePlace the sample on the magnetic stand so that the magnetic beads adhere to the tube wall;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdsorb using a single-well magnetic stand (3 min)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdsorb using a multi-well magnetic stand (5 min)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTilt the magnetic stand and pour the sample into a new collection tube to collect the sample;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eThe sorted cells can be centrifuged at 400 g for 7 minutes, for subsequent culture and analysis.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCell sorting successful\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003e1.\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e \u003c\/span\u003e\u003cspan\u003e0.1-1 × 10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ecells (0.1-1 mL) are recommended to use a 5 mL single-well magnetic separator (\u003c\/span\u003e\u003cspan style=\"text-decoration:underline\"\u003eStarter EasyIso Separator\u003c\/span\u003e\u003cspan\u003e) with a total adsorption volume of 2.5 mL;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2-5 × 10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003eFor cells (2–5 mL), it is recommended to use a 15 mL multi-well magnetic separator (\u003c\/span\u003e\u003cspan style=\"text-decoration:underline\"\u003eStarter EasyEights EasyIso Separator\u003c\/span\u003e\u003cspan\u003e) The total adsorption volume is 7.5 mL;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2. If the sample viability is low, it is recommended to use a dead cell removal kit to eliminate dead cells (Dead Cell Removal Set, (RUO)#S0K0009);\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"100T","offer_id":42917951438923,"sku":"S0K3014-100T","price":1000.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/779afb131f6942579be3b4556e5c4b8e.png?v=1788246176"},{"product_id":"t-cell-activationexpansion-kit-mouseruo-s0k1016","title":"T Cell Activation\/Expansion Kit, mouse（RUO）","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e2 mL Streptavidin NanoBeads, in a PBS buffer containing BSA and Poloxamer 188;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e0.4 mL CD3ε-Biotin, mouse (100 µg\/mL), in a PBS buffer containing BSA;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e0.4 mL CD28-Biotin, mouse (100 µg\/mL), in a PBS buffer containing BSA;\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eCan activate 2 × 10⁸ T cells;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003eThis kit is used for the in vitro activation and expansion of mouse T cells, with its core components consisting of streptavidin magnetic beads and biotin‑labeled mouse CD3ε\/CD28 antibodies.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAfter biotinylated CD3ε and CD28 antibodies are conjugated to the surface of streptavidin‑nanomagnetic beads, the beads can mimic antigen‑presenting cells, stimulating the activation of single‑cell suspensions from the spleen or lymph nodes, or purified resting T cells; on days 6–8 of culture, they can be restimulated to achieve large‑scale T‑cell expansion.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eApplications in cell sorting\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eThis product is intended for research use only and is not to be used for clinical diagnosis or treatment.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eMouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e150 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8 °C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan\u003eMouseTLymphocytes are the core effector cells of the adaptive immune system, mainly divided intoCD4+helperTcells andCD8+cytotoxicTcells, playing a key role in anti-infectious immunity, tumor immunity, immune regulation, and the development of autoimmune diseases. Under physiological conditions, naïveTcells in the mouse spleen and lymph nodes are in a quiescent, dormant state, barelyproliferating,expressing low levels of functional molecules, and thus unable to directly conduct in vitro functional experiments. Therefore, controlledTcell activation and large-scale expansion in vitro are core foundational technologies for studying murine immune mechanisms, validating cell functions, and constructing immune models; this reagentbox employsartificially simulated antigen-presenting cell technology, using biotinylatedCD3εandCD28antibody-conjugated anti-biotin magnetic beads to precisely replicateTcell activation's dual signals in vitro, achieving highly efficient, non-specific activation and expansion of polyclonalTcells, suitable for total mouseTcells,CD4T,CD8 TMass amplification of cells. Magneticbeads serve assolid-phase carriers, capable of simultaneously enriching large quantities ofCD3εandCD28antibodies, stably binding to the corresponding receptors on the surface ofTcells, continuously and evenly providing the first and second signals, thereby avoiding the instability inherent in natural antigen presentation and addressingprimary mouseTcell issues of poor in vitro activation and weak proliferation specific to certain species, significantly enhancing activation efficiency and expansion multiples.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003e1.\u003c\/span\u003e \u003cspan style=\"font-weight:bold\"\u003eExperimental reagents\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1.1\u003c\/span\u003e \u003cspan\u003eBuffer:pH 7.2 PBSbuffer, with the addition of2 mM EDTA,2–8℃Pre-cooled; do not use buffers containing calcium or magnesium ions\/Culture medium;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1.2\u003c\/span\u003e \u003cspan\u003eCell culture medium: RPMI 1640, with the addition of10%fetal bovine serum (FBS)+IL-2(final concentration30–100 IU\/mL);Optional addition of50-100μMβ-mercaptoethanol, to maintain cell viability under rapid proliferation conditions;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003e2.\u003c\/span\u003e \u003cspan style=\"font-weight:bold\"\u003eMagnetic bead conjugation (conjugatingCD3ε\/CD28antibodies)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe magnetic beads contain no preservatives; all aliquoting operations must be performed under sterile conditions; it is recommended to conjugate500 μLof magnetic beads at a time, and after conjugation, the beads2–8℃can be stably stored for3months without washing away unbound antibodies.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.1\u003c\/span\u003e \u003cspan\u003eTake2 mLof a sterile centrifuge tube, and add100μLCD3ε-Biotin + 100μLCD28-Biotin, mix thoroughly by pipetting; \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.2\u003c\/span\u003e \u003cspan\u003eadd300μL buffer (pH 7.2 PBScontaining2 mM EDTA) and mix well;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.3\u003c\/span\u003e \u003cspan\u003ethoroughly vortexto resuspend the magnetic beads, ensuring uniform dispersion of the beads; then pipette500μLStreptavidinNanoBeadsadd to the above antibody mixture, bringing the total volumeto 1 mL;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.4\u003c\/span\u003e \u003cspan\u003eplace the centrifuge tube on a rotator,at 2–8℃and incubate with gentle continuous rotationfor 1 hour; this results in coupledCD3ε\/CD28magnetic beads, which can be used directly afterward; \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003e3. Experimental steps\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAll operations must be performed in a sterile environment.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3.1\u003c\/span\u003e \u003cspan\u003eSample preparation\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eResting mouse T cells derived from the spleenTCell activation and expansion, preparing single-cell suspensions from lymphoid organs (spleen, lymph nodes). Magnetic bead sorting can be used.TCell activation and expansion: positive selectionCD3\/CD4\/CD8 T cells:CD3ε Nanobeads Kit, mouse (RUO)\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e(Cat. No. S0K0015);CD4 Nanobeads,Mouse(RUO) (Cat. No. S0K0003);CD8 Nanobeads, Mouse(RUO)(Cat. No. S0K0005);negative selec-tionCD3\/ CD4\/CD8T cells::Mouse T Cell Isolation Kit(Cat. No. S0K1008\/S0K3006);Mouse CD4 T Cell Isolation Kit(Cat. No. S0K1005\/S0K3003);\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eMouse CD8 T Cell Isolation Kit (Cat. No. S0K1007\/S0K3004).\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eRefer to the corresponding product manual for specific operations.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3.2TCell activation and expansion procedures\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe basic system in this protocol is suitable for1×10⁶cellsafter sortingTcellscorresponding to20μLof coupled magnetic beads; when the cell quantity doubles, all reagent volumes are scaled up accordingly.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003e3.2.1\u003c\/span\u003e \u003cspan style=\"color:#000000\"\u003eTake the sorted cells at a concentration of1×10⁶\/mLand resuspend them infresh complete culture medium (RPMI1640 + 10% FBS + 30–100 IU\/mL IL-2)in,take1mLof cellsinto a centrifuge tube,thoroughly mix the coupled magnetic beads, then add20μLof the coupledmagnetic beads,centrifuge at400 gfor7 min;(negative control: use uncoupled blank magnetic beads instead ofcoupledmagnetic beads;)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3.2.2\u003c\/span\u003e \u003cspan\u003eAfter centrifugation, discard the supernatant and resuspend the pellet in1 mLFresh complete medium (RPMI1640 + 10% FBS + 30–100 IU\/mL IL-2),Add the cells to the corresponding plates for culture. To ensure optimal stimulation and proliferation, it is recommended to use a standard seeding density:1×10⁶cells\/cm²;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eOptimal Culture System Ratio Table for Each Culture Plate\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse:collapse;width:auto;border:1px solid #000000\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003eCulture Plate Model\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003eGrowth Base Area\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003eTotal CultureVolume\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003eTCell Seeding Quantity\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold;color:#000000\"\u003eAmount of Coupled Magnetic Beads\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e96Well Plate\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e0.31 cm²\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e0.2 mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e0.25×10⁶\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e5 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e48Well Plate\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e1 cm²\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e1 mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e1×10⁶\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e20μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e24Well Plate\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e2 cm²\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e2 mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e2×10⁶\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e40μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e12Well Plate\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e4 cm²\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e4 mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e4×10⁶\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e80μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e6Well Plate\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e10 cm²\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e5 mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e5×10⁶\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"color:#000000\"\u003e100μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3.2.3\u003c\/span\u003e \u003cspan\u003ePlace the culture plate ina CO₂incubator for cultivation, observe cell status daily, and replenish the medium promptly when it becomes insufficient;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3.2.4\u003c\/span\u003e \u003cspan\u003eAfter culturing for2days, gently disperse cell clumps; at this step, samples can be taken for flow cytometry analysis.mouse CD25, mouse CD69to assess cell activation status.;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3.2.5\u003c\/span\u003e \u003cspan\u003ePassage and expansion: every2days, subculture at a ratio of1:2or1:4proportion, add fresh culture medium, and if proliferation is too rapid, shorten the passage interval.;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3.2.6\u003c\/span\u003e \u003cspan\u003eSecondary stimulation: after activating the culture for6–8days,Tcells enter a quiescentstate,if continued expansion is needed, magnetic beads must be added again to restimulate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3.3\u003c\/span\u003e \u003cspan\u003eMagnetic bead removal procedure\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eIf downstream operations require magnetic bead sorting, replacement of stimulating antigens, or stimulants, the magnetic beads must be removed in advance:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3.3.1\u003c\/span\u003e \u003cspan\u003eCollect the cells and transfer them to5\/15\/50 mLCentrifuge the tube and wash once with buffer;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3.3.2\u003c\/span\u003e \u003cspan\u003eResuspend the cells in buffer, at a maximum concentration2×10⁷cells\/mL, and pipette thoroughly to mix evenly;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3.3.3\u003c\/span\u003e \u003cspan\u003eFix the centrifuge tube on the magnetic separator and let it stand for adsorption10 min;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003e3.3.4\u003c\/span\u003e \u003cspan\u003eTransfer the cells to a new centrifuge tube, collect the bead-free cells, and proceed with subsequent experiments.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"200T","offer_id":42920108884043,"sku":"S0K1016-200T","price":420.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/e3cd042cbb2b4a40b4f93ff200030650.jpg?v=1788249620"},{"product_id":"mouse-t-cell-isolation-kit-column-free-s0k3006","title":"Mouse T Cell Isolation Kit (Column-Free)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e1 mL Mouse T Cell Biotin-Antibody Cocktail\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e0. 5 mL Streptavidin Beads\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eSuitable for a total cell count of 1×10⁹, and can perform up to 100 sorting runs.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eIn this negative selection process, non- is labeled with a biotin-conjugated antibody.\u003c\/span\u003e\u003cspan style=\"color:#404040\"\u003eT\u003c\/span\u003e\u003cspan\u003eCells are then incubated with streptavidin‑coated nanomagnetic beads; non‑target cells are labeled via antibody‑bead complexes, while the target…\u003c\/span\u003e\u003cspan style=\"color:#404040\"\u003eT\u003c\/span\u003e\u003cspan\u003eThe cells remain unlabeled. The cell suspension is then transferred to a polystyrene tube and placed within the sorter's magnetic field. Under the influence of the magnetic field, the magnetically labeled non-target cells adhere to the tube wall, while the unlabeled cells…\u003c\/span\u003e\u003cspan style=\"color:#404040\"\u003eT\u003c\/span\u003e\u003cspan\u003eThe cells remain in the supernatant. This supernatant is, therefore, the fraction obtained after removing the non-target cells.\u003c\/span\u003e\u003cspan style=\"color:#404040\"\u003eT\u003c\/span\u003e\u003cspan\u003eCell-enriched fraction.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eMouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e150 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eNegative Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8 °C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"color:#000000\"\u003eMouse T lymphocytes are the most central effector cell population in the murine adaptive immune system, serving as a key force in defending the body against pathogen invasion, eliminating abnormal cells, and maintaining immune homeostasis. The CD3 molecule is the most characteristic transmembrane glycoprotein complex on the surface of mouse T cells, composed of four polypeptide chains—γ, δ, ε, and ζ—linked by non-covalent bonds. Together with the T-cell receptor (TCR), they form the TCR–CD3 complex. Although the CD3 molecule does not directly recognize antigens, its primary function is to transmit the activation signals generated by TCR–antigen interactions into the cell, thereby initiating T-cell activation, proliferation, and differentiation. It is an essential prerequisite for T-cell maturation, activation, and functional execution; consequently, the CD3 molecule is often used as a specific surface marker for identifying T cells. In scientific research, obtaining highly pure, high‑activity mouse T cells is a prerequisite for conducting T‑cell‑related studies. This negative selection kit employs the principle of negative sorting: biotin‑conjugated antibodies specifically label non‑CD3⁺ T cells (such as B cells, macrophages, and granulocytes), which are then bound and removed using streptavidin‑coated magnetic nanoparticles. Throughout the process, there is no binding to T‑cell surface antigens, thus maximizing the preservation of their native morphology, the integrity of surface markers, and functional activity, providing reliable cellular material for subsequent experiments.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cspan style=\"display:inline; white-space:nowrap;\"\u003eNote: This kit does not include sorting buffer; please prepare it yourself. The corresponding Stater product number is\u003ca href=\"https:\/\/www.starter-bio.com\/s0d3018_starter_magsep_separation_buffer.html\" style=\"color:#0066FF; display:inline; white-space:nowrap;\"\u003eS0D3018\u003c\/a\u003e\u003c\/span\u003e\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eSteps\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eOperating Instructions\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eDosage and Time\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTake the mouse spleen, grind it on a 40 μm sterile filter to prepare a single-cell suspension of splenocytes; proceed with cell counting;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eIt is recommended to count cells after staining with AO\/PI; splenocytes can be centrifuged at 400 g for 7 minutes;\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eSample Preparation\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eResuspend 1×10 in 100μL MagSep Separation Buffer\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ecells;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eReagents should be adjusted proportionally according to the sample volume; set aside a pre-sorting sample to determine the initial Mouse T ratio;\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ecells\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 10 μL of Mouse T cell Biotin Antibody Cocktail to the sample and gently mix.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eFor this step, gentle pipetting 2–3 times can be used to mix thoroughly.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10 μL \/ 100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate the antibodies in the cocktail with the cells at room temperature for 10 min;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate at room temperature for 10 min\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eShake for 5–30 s to mix the Streptavidin beads thoroughly;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eMix for 5–30 s\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e6\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 5 μL of Streptavidin beads to the sample;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5 μL\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eGently mix the magnetic beads and cells, and incubate at room temperature for 5 minutes;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eIn this step, you can use a pipette to gently blow the mixture 2–3 times for mixing;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDuring this step, the magnetic beads may settle at the bottom; you can lightly tap the tube to remix them around the 2.5-minute mark;\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate at room temperature for 5 minutes\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd MagSep Separation Buffer to the sample and immediately place it on the magnetic stand to prevent the magnetic beads from settling heavily at the bottom;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eBring the total volume up to 2.5 mL (using a 5 mL flow cytometry tube)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd the total volume to 7.5 mL (in a 15 mL centrifuge tube)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e9\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003ePlace the sample on the magnetic stand so that the magnetic beads adhere to the tube wall;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdsorb using a single-well magnetic stand (3 min)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdsorb using a multi-well magnetic stand (5 min)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTilt the magnetic stand and pour the sample into a new collection tube to collect it;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eNote: The sorted cells can be centrifuged at 400g for 7 minutes for subsequent culture and analysis.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCell sorting successful\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote: 1.\u003c\/span\u003e\u003cspan\u003eFor 0.1–1 × 10^8 cells (0.1–1 mL), it is recommended to use a 5 mL single-well magnetic separator (\u003c\/span\u003e\u003cspan style=\"text-decoration:underline\"\u003eStarter EasyIso Separator\u003c\/span\u003e\u003cspan\u003e) with a total adsorption volume of 2.5 mL;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eFor 2–5 × 10^8 cells (2–5 mL), it is recommended to use a 15 mL multi-well magnetic separator (\u003c\/span\u003e\u003cspan style=\"text-decoration:underline\"\u003eStarter EasyEights EasyIso Separator\u003c\/span\u003e\u003cspan\u003e) with a total adsorption volume of 7.5 mL;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e2.\u003c\/span\u003e\u003cspan\u003eIf the live cell rate in the sample is low, it is recommended to use a dead cell removal kit to eliminate dead cells (\u003c\/span\u003e\u003cspan style=\"text-decoration:underline\"\u003eDead Cell Removal Set, (RUO)\u003c\/span\u003e\u003cspan\u003e#S0K0009);\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"100T","offer_id":42955273044043,"sku":"S0K3006-100T","price":565.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/6310c90c49cc487db4878a15dc443585.jpg?v=1788184955"},{"product_id":"human-t-cell-panel-kit-s0n0017","title":"Human T Cell Panel Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eFCM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHu\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePositive Sample\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman peripheral blood cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003ePBS, 1% BSA, 0.09% sodium azide\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e12 months from date of receipt \/ reconstitution, 2 to 8 °C as supplied.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan\u003eThis four\u003c\/span\u003e\u003cspan\u003e‑\u003c\/span\u003e\u003cspan\u003ecolor panel is designed for detecting T\u003c\/span\u003e\u003cspan\u003e‑\u003c\/span\u003e\u003cspan\u003ecell subpopulations in\u003c\/span\u003e\u003cspan\u003e human peripheral blood or other biological samples\u003c\/span\u003e\u003cspan\u003e. CD45 serves as a pan-leukocyte marker. CD3 was included as a general T\u003c\/span\u003e\u003cspan\u003e‑\u003c\/span\u003e\u003cspan\u003ecell marker, and CD4 and CD8 were included to identify helper T\u003c\/span\u003e\u003cspan\u003e‑\u003c\/span\u003e\u003cspan\u003ecell and cytotoxic T\u003c\/span\u003e\u003cspan\u003e‑\u003c\/span\u003e\u003cspan\u003ecell subpopulations. The panel is compatible with most t\u003c\/span\u003e\u003cspan\u003ehree\u003c\/span\u003e\u003cspan\u003e‑\u003c\/span\u003e\u003cspan\u003elaser flow cytometers. If desired, the panel can serve as a backbone panel, allowing for the addition of antibodies conjugated to various fluorophores to detect other markers of interest.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e","brand":"Starter","offers":[{"title":"50T","offer_id":42968334827595,"sku":"S0N0017-50T","price":585.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/a1c513e701e84fbf8c29bb6baea9c177.png?v=1788253352"},{"product_id":"ipschipsc-ua090059","title":"hiPSC-to-Mast Cell Differentiation Cytokine Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLyophilized powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReconstitution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan style=\"background-color:yellow\"\u003eReconstitute at 0.1-1 mg\/ml according to the size in ultrapure water after rapid centrifugation.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e·12 months from date of receipt, lyophilized powder stored at -20 to -80℃. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e· 3 months, -20 to -80℃ under sterile conditions after reconstitution. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e· 1 week, 2 to 8℃ under sterile conditions after reconstitution. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e· Please avoid repeated freeze-thaw cycles.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;\" border=\"0\" cellspacing=\"0\" cellpadding=\"0\" width=\"595\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height:33.0pt;\"\u003e\n\u003ctd style=\"border:solid black 1.0pt;height:33.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:191.0pt;\" width=\"255\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:top;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eComponent\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid black 1.0pt;height:33.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:71.0pt;\" width=\"95\" valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:top;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eReference dosage\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid black 1.0pt;height:33.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:87.5pt;\" width=\"117\" nowrap\u003e\u003cp style=\"text-align:left;vertical-align:middle;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eS Size(100ml system)\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid black 1.0pt;height:33.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:97.0pt;\" width=\"129\" nowrap\u003e\u003cp style=\"text-align:left;vertical-align:middle;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eM Size(1L system)\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:16.5pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid black 1.0pt;height:16.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:191.0pt;\" width=\"255\" nowrap\u003e\u003cp style=\"text-align:left;vertical-align:middle;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eFGF-basic(154aa) Protein, Human\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:16.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:71.0pt;\" width=\"95\" nowrap\u003e\u003cp style=\"text-align:left;vertical-align:middle;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e1-2 ng\/mL\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:16.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:87.5pt;\" width=\"117\" nowrap\u003e\u003cp style=\"text-align:left;vertical-align:middle;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e5\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;'\u003eμ\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eg\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:16.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:97.0pt;\" width=\"129\" nowrap\u003e\u003cp style=\"text-align:left;vertical-align:middle;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e5\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;'\u003eμ\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eg\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:16.5pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid black 1.0pt;height:16.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:191.0pt;\" width=\"255\" nowrap\u003e\u003cp style=\"text-align:left;vertical-align:middle;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eIL-6 Protein, Human\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:16.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:71.0pt;\" width=\"95\" nowrap\u003e\u003cp style=\"text-align:left;vertical-align:middle;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e100 ng\/mL\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:16.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:87.5pt;\" width=\"117\" nowrap\u003e\u003cp style=\"text-align:left;vertical-align:middle;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e10\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;'\u003eμ\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eg\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:16.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:97.0pt;\" width=\"129\" nowrap\u003e\u003cp style=\"text-align:left;vertical-align:middle;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e100\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;'\u003eμ\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eg\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:16.5pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid black 1.0pt;height:16.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:191.0pt;\" width=\"255\" nowrap valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:top;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eSCF Protein,Human\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:16.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:71.0pt;\" width=\"95\" nowrap\u003e\u003cp style=\"text-align:left;vertical-align:middle;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e100 ng\/mL\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:16.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:87.5pt;\" width=\"117\" nowrap\u003e\u003cp style=\"text-align:left;vertical-align:middle;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e10\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;'\u003eμ\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eg\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:16.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:97.0pt;\" width=\"129\" nowrap\u003e\u003cp style=\"text-align:left;vertical-align:middle;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e100\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;'\u003eμ\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eg\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:16.5pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid black 1.0pt;height:16.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:191.0pt;\" width=\"255\" nowrap valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:top;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eFLT-3L Protein,Human\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:16.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:71.0pt;\" width=\"95\" nowrap\u003e\u003cp style=\"text-align:left;vertical-align:middle;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e25 ng\/mL\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:16.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:87.5pt;\" width=\"117\" nowrap\u003e\u003cp style=\"text-align:left;vertical-align:middle;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e5\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;'\u003eμ\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eg\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:16.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:97.0pt;\" width=\"129\" nowrap\u003e\u003cp style=\"text-align:left;vertical-align:middle;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e5\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;'\u003eμ\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eg\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:16.5pt;\"\u003e\n\u003ctd style=\"border-top:none;border:solid black 1.0pt;height:16.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:191.0pt;\" width=\"255\" nowrap valign=\"top\"\u003e\u003cp style=\"text-align:left;vertical-align:top;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eIL-3 Protein,Human\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:16.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:71.0pt;\" width=\"95\" nowrap\u003e\u003cp style=\"text-align:left;vertical-align:middle;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e5 ng\/mL\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:16.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:87.5pt;\" width=\"117\" nowrap\u003e\u003cp style=\"text-align:left;vertical-align:middle;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e5\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;'\u003eμ\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eg\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid black 1.0pt;border-left:none;border-right:solid black 1.0pt;border-top:none;height:16.5pt;padding:0cm 5.4pt 0cm 5.4pt;width:97.0pt;\" width=\"129\" nowrap\u003e\u003cp style=\"text-align:left;vertical-align:middle;\" align=\"left\"\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003e5\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;'\u003eμ\u003c\/span\u003e\u003cspan style='font-family:\"微软雅黑\",sans-serif;font-size:11.0pt;' lang=\"EN-US\"\u003eg\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e \u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eProtocol for Differentiation of Human Induced Pluripotent Stem Cells (hiPSCs) into Mast Cells\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1. Routine Culture of Human Induced Pluripotent Stem Cells (hiPSCs)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1.1 Seeding and Coating: Human induced pluripotent stem cells (hiPSCs) were seeded onto six-well plates pre-coated with Matrigel .\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1.2 Medium and Culture Conditions: Cells were routinely cultured in mTeSR™ Plus complete medium at 37 °C in a 5% CO₂ humidified incubator. Medium changes and passaging were performed according to the growth status of the cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2. Induction of Embryoid Body (EB) Formation\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.1 Dissociation and Collection: When the iPSCs reached 70–90% confluence, the culture medium was aspirated, and the cells were gently rinsed once with 1 mL of PBS. After removing the PBS, 1 mL of mTeSR™ Plus dissociation reagent was added and incubated at room temperature for 30 seconds, The ReLeSR™ reagent was then aspirated.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.2 Preparation of Cell Clumps: The plates were incubated at 37 °C in a 5% CO₂ incubator for 5 minutes. Then, 1 mL of mTeSR1 medium was added , and the culture surface was gently pipetted to disperse the cells into appropriately sized sheet-like or clump aggregates. Formation of a single-cell suspension should be avoided.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.3 Suspension Culture and EB Induction: For cells from one T25 flask, the cell clumps were transferred to ultra-low attachment six-well plates at a 1:8 split ratio and cultured in EB induction medium (formula detailed below). The cells were cultured in suspension for 5 days, with medium changes performed every 2–3 days.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.4 EB Induction Medium Composition: The EB induction medium consisted of DMEM\/F12 basal medium, 20% KnockOut™ Serum Replacement (KSR), 1% Non-Essential Amino Acids (NEAA), 1% GlutaMax™, 0.1 mM 2-mercaptoethanol, and 1–2 ng\/mL bFGF. (Note: 10 μM Y-27632 was supplemented during the first 24–48 hours of induction to enhance cell survival.)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3. Establishment and Culture of Myeloid Cell-Forming Complexes (MCFCs)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3.1 Transfer and Seeding: On day 6 of EB culture, EBs were collected using a cut pipette tip and seeded onto 6-well plates pre-coated with 0.1% gelatin at a density of 25–30 EBs per well.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3.2 MCFC Induction Culture: Mast Cell Medium I, as described below, was added to the cultures. The cells were then maintained for an additional 6–7 days to promote the formation of myeloid cell-forming complexes (MCFCs) and the generation of hematopoietic progenitor cells..\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3.3 Mast Cell Media I Composition: Mast Cell Medium I consisted of APEL medium (STEMCELL, Cat. No. 05270), 100 ng\/mL IL-6, 100 ng\/mL SCF, 25 ng\/mL Flt-3L, and 5 ng\/mL IL-3.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e4. Harvesting of Suspended Cells and Phenotypic Analysis\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e4.1 Observation of Release Phase: One week after EB seeding, the MCFCs began to gradually release suspended cells into the medium. The number of released suspended cells progressively increased during weeks 2–4 of culture.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e4.2 Collection and Detection: To harvest the suspended cells, the side of the culture plate was gently tapped to facilitate the release of cells. The supernatant containing the suspended cells was collected. After centrifugation, the harvested cells were subjected to flow cytometry for the detection of surface markers (e.g., CD34, CD45, CD123, c-Kit, and FcεRI) to evaluate the induction of hematopoietic progenitors.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e5. Maturation Induction of Mast Cells\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e5.1 Medium Change and Induction: The suspension cells released from MCFC into the culture medium were collected. After centrifugation and resuspension, the cells were transferred to Mast Cell Media II (MC Medium II; formula provided below) for maturation induction. The MCFC cultures were replenished with fresh Mast Cell Media I and maintained, and the suspension cells were collected once per week.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e5.2 Maturation Culture: The cells were cultured in Mast Cell Media II for 2–3 weeks to promote terminal differentiation into mature mast cells. During the final week of culture, the cytokine concentration could be doubled to further facilitate mast cell maturation.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e5.3 Quality Assessment: Following the maturation induction, cells were harvested and analyzed by flow cytometry to confirm mast cell identity by evaluating the expression of maturation markers, including c-Kit, FcεRI, CD45RA, and CD123.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e5.4 Mast Cell Media II Composition: Mast Cell Medium II consisted of RPMI 1640 medium (containing GlutaMax™), 10% fetal bovine serum (FBS), 1% penicillin-streptomycin, 1 mM sodium pyruvate, 1% NEAA, 100 ng\/mL SCF, and 100 ng\/mL IL-6.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eGuidelines\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"margin-bottom: 0px;\"\u003estore in separate containers to reduce the number of freeze-thaw cycles.\u003c\/p\u003e\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"S","offer_id":43002134036555,"sku":"UA090059-S","price":435.0,"currency_code":"USD","in_stock":true},{"title":"M","offer_id":43002134069323,"sku":"UA090059-M","price":1285.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/3d1e121a89c14834a7d6c45a2d5811af.jpg?v=1788260442"},{"product_id":"human-nave-cd4-t-cell-isolation-kit-column-based-s0k1014","title":"Human Naïve CD4 T Cell Isolation Kit (Column-Based)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e1 mL Human Naïve CD4 T Cell Biotin-Antibody Cocktail, PBS buffer containing BSA;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1.5 mL Streptavidin Beads, PBS buffer containing BSA and Poloxamer 188;\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eSuitable for a total cell count of 1×10⁹, and can perform up to 100 sorting runs.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eThis kit employs a column-based negative selection approach to isolate Human Naïve CD4 T cells from human peripheral blood mononuclear cells (PBMCs). In this negative-selection process, non-Human Naïve CD4 T cells are labeled with biotin-conjugated antibodies, and the cells are then incubated with streptavidin-nanomagnetic beads. As a result, the unwanted cells become bound to the magnetic beads via the antibody‑bead complex, while the target Human Naïve CD4 T cells remain unlabeled. The cell suspension is subsequently loaded onto the separation column; under the influence of a magnetic field, the magnetically labeled non-target cells are retained within the column, whereas the unlabeled Human Naïve CD4 T cells elute by gravity into the collection tube. The resulting supernatant represents the enriched fraction of Human Naïve CD4 T cells after removal of the non-target cells.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eApplications in cell sorting\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eNaïve CD4 T cells in their unmanipulated, label-free state are isolated from human PBMCs and can be used for a variety of downstream functional assays.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eNegative Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8 °C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan\u003eThe immunophenotype of human naïve CD4 T cells is CD3⁺CD4⁺CD45RA⁺CD45RO⁻. They constitute the core precursor population of helper T cells in the human adaptive immune system, responsible for initial antigen recognition and directed differentiation, and are also a key cell subset for maintaining immune homeostasis, mediating specific responses, and establishing immunological memory. The human naïve CD4 T-cell negative-selection kit is developed based on immuno-magnetic bead–mediated negative selection, overcoming the labeling limitations inherent in positive-selection methods. By specifically depleting non-target cells—such as CD8 T cells, NK cells, B cells, monocytes, dendritic cells, granulocytes, and memory T cells—it yields unlabeled, native-state, highly purified naïve CD4 T cells, perfectly preserving their initial quiescent state, surface molecule integrity, and multipotential differentiation capacity. This makes them suitable for various functional assays, including in vitro activation and proliferation, subtype-directed differentiation induction, and cytokine secretion analysis, and they have become the mainstream research tool for isolating human naïve CD4 T cells.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cspan style=\"display:inline; white-space:nowrap;\"\u003eNote: This kit does not include sorting buffer; please provide it separately. The corresponding Staret product number is\u003ca href=\"https:\/\/www.starter-bio.com\/s0d3018_starter_magsep_separation_buffer.html\" style=\"color:#0066FF; display:inline; white-space:nowrap;\"\u003eS0D3018\u003c\/a\u003e\u003c\/span\u003e\u003cp\u003e\u003cspan\u003ewith\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eL Separation Column\u003c\/span\u003e\u003cspan\u003eFor example\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eSteps\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eOperating Instructions\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eDosage and timing\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"6\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eCell processing and labeling\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003ePeripheral blood mononuclear cells (PBMCs) are isolated from peripheral blood by density-gradient centrifugation using lymphocyte separation medium, or cryopreserved PBMCs are directly thawed and resuspended, followed by cell counting.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eIt is recommended to count cells after staining with AO\/PI; PBMCs can be pelleted by centrifugation at 400 g for 7 minutes.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003ePrepare the sample\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eResuspend 1×10 in 100 μL of MagSep Separation Buffer.\u003c\/span\u003e\u003csup\u003e\u003cspan\u003eseven\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003eCell;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eReagents are adjusted proportionally based on the sample volume; a pre-sorting sample is retained for assessing the proportion of human naïve CD4 T cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003eseven\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ecells\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 10 μL of the Human Naïve CD4 T Cell Biotin Antibody Cocktail to the sample and gently mix.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eIn this step, gently pipette up and down 2–3 times to mix thoroughly;\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10 μL\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate the antibodies in the cocktail with the cells at room temperature for 10 min;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eIf you want a higher cell yield, after antibody incubation, add twice the volume of MagSep Separation Buffer, centrifuge at 400 g for 7 min, then carefully discard the supernatant. Resuspend 1×10 in 100 μL of MagSep Separation Buffer.\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ecells, and proceed to the next steps.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate at room temperature for 10 min\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eMix well\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eStreptavidin Beads\u003c\/span\u003e\u003cspan\u003e, add 15 μL\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eStreptavidin Beads\u003c\/span\u003e\u003cspan\u003eto the sample;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e15 μL\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e6\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eGently mix the magnetic beads and cells, and incubate at room temperature for 8 minutes;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate at room temperature for 8 minutes\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eCell sorting\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIn the column separation magnet, install\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eL Separation Column\u003c\/span\u003e\u003cspan\u003e, add 3 mL of MagSep Separation Buffer to wash the separation column;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eWash the separation column\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd the cells to the separation column and collect the target cells using a collection tube;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eThe cell volume should be at least 500 μL; if insufficient, make up the volume with MagSep Separation Buffer; the cells that drip down under gravity are the target cells;\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCollect the target cells\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e9\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 3 mL of MagSep Separation Buffer and collect the target cells;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eNote: This step elutes the unlabeled target cells from the separation column, increasing the yield.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCollect the target cells\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"4\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eNotes: 1. Pay attention to the maximum capacity of the separation column,\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eThe S Separation Column can label a maximum of\u003c\/span\u003e\u003cspan\u003e1×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ecells;\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eThe L Separation Column can label a maximum of\u003c\/span\u003e\u003cspan\u003e1×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ecells;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2. If the sample viability is low, it is recommended to use a dead cell removal kit to eliminate dead cells (Dead Cell Removal Set, (RUO)#S0K0009);\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"100T","offer_id":43030515810379,"sku":"S0K1014-100T","price":1000.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/e4b99aedf25946b9b3973f91ebefd2bc.png?v=1788210041"},{"product_id":"human-cd4-t-cell-isolation-kit-column-free-s0k3009","title":"Human CD4 T Cell Isolation Kit (Column-Free)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e1 mL Human CD4 T Cell Biotin-Antibody Cocktail\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1mL Streptavidin Beads\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eSuitable for a total cell count of 1×10⁹, and can perform up to 100 sorting runs.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eIn this negative selection process, non-CD4 T cells are labeled with biotin-conjugated antibodies, and the cells are then incubated with streptavidin-coated nanomagnetic beads. As a result, the non-target cells become bound to the magnetic beads via the antibody‑antigen complex, while the target CD4 T cells remain unlabeled. Subsequently, the cell suspension is transferred to a polystyrene tube and placed within the magnetic field of the sorter. Under the influence of the magnetic field, the magnetically labeled non-target cells adhere to the tube wall, whereas the unlabeled CD4 T cells remain in the supernatant. This supernatant constitutes the T-cell‑enriched fraction after removal of the non-target cells.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e150 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eNegative Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8 °C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eHuman CD4 T cells are key immune cells in the immune system, playing a central role in immune responses, immune regulation, and defense against infection and tumors. This kit employs a column-free negative selection approach: non-CD4 T cells are labeled with biotin-conjugated antibodies, and unwanted cells are removed using magnetic bead separation, thereby enriching highly pure, high‑activity CD4 T cells. This method avoids the potential impact of direct labeling on CD4 T cell function and is suitable for downstream experimental studies such as cell culture and functional assays.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cspan style=\"display:inline; white-space:nowrap;\"\u003eNote: This kit does not include sorting buffer; please provide it separately. The corresponding Staret product number is\u003ca href=\"https:\/\/www.starter-bio.com\/s0d3018_starter_magsep_separation_buffer.html\" style=\"color:#0066FF; display:inline; white-space:nowrap;\"\u003eS0D3018\u003c\/a\u003e\u003c\/span\u003e\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eSteps\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eOperating Instructions\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eDosage and timing\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003ePeripheral blood mononuclear cells (PBMCs) are isolated from peripheral blood by density-gradient centrifugation using lymphocyte separation medium, or cryopreserved PBMCs are directly thawed and resuspended, followed by cell counting.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eNote: It is recommended to count cells after staining with AO\/PI; PBMCs can be centrifuged at 400g for 7 minutes.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003ePrepare the sample\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eResuspend 1×10 in 100 μL of MagSep Separation Buffer.\u003c\/span\u003e\u003csup\u003e\u003cspan\u003eseven\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003eCell;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eReagents are adjusted proportionally based on the sample volume; a pre-sorting sample is retained for determining the proportion of human CD4 T cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003eseven\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ecells\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 10 μL of the Human CD4 T cell Biotin Antibody Cocktail to the sample and gently mix.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eIn this step, gently pipette up and down 2–3 times to mix thoroughly;\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10 μL\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate the antibodies in the cocktail with the cells at room temperature for 10 minutes;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate at room temperature for 10 minutes\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eShake for 5–30 seconds to mix the Streptavidin beads;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eShake for 5–30 seconds\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e6\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 10 μL of Streptavidin beads to the sample;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10 μL\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eGently mix the magnetic beads and cells, and incubate at room temperature for 5 minutes;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eFor mixing in this step, you can use a pipette to gently blow the mixture 2–3 times;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDuring this step, the magnetic beads may settle at the bottom; you can mix them again after about 2.5 minutes;\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate at room temperature for 5 minutes\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd MagSep Separation Buffer to the sample and immediately place it on the magnetic stand to prevent the beads from settling excessively;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd the total volume to 2.5 mL (5 mL flow cytometry tube)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd the total volume to 7.5 mL (15 mL centrifuge tube)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e9\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003ePlace the sample on the magnetic stand so that the magnetic beads adhere to the tube wall;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdsorb using a single-well magnetic stand (3 min)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdsorb using a multi-well magnetic stand (5 min)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTilt the magnetic stand and pour the sample into a new collection tube to collect the sample;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eThe sorted cells can be centrifuged at 400g for 7 minutes, for subsequent culture and analysis.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCell sorting successful\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eFor 0.1–1 × 10^8 cells (0.1–1 mL), it is recommended to use a 5 mL single-well magnetic separator (Starter EasyIso Separator) with a total volume of 2.5 mL;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2–5 × 10^8 cells (2–5 mL) are recommended to be adsorbed using a 15 mL row-type magnetic separator (Starter EasyEights EasyIso Separator) with a total volume of 7.5 mL;\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"100T","offer_id":43030515875915,"sku":"S0K3009-100T","price":1000.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/e103a9b4c9cd41ea89a4f4a92614a5ef.jpg?v=1788210034"},{"product_id":"human-cd8-t-cell-isolation-kit-column-free-s0k3008","title":"Human CD8 T Cell Isolation Kit (Column-Free)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e1 mL Human CD8 T Cell Biotin-Antibody Cocktail\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1mL Streptavidin Beads\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eSuitable for a total cell count of 1×10⁹, and can perform up to 100 sorting runs.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eIn this negative selection process, non-CD8 T cells are labeled with biotin-conjugated antibodies, and the cells are then incubated with streptavidin-nanomagnetic beads. As a result, the non-target cells are marked by the antibody‑bead complex, while the target CD8 T cells remain unlabeled. Subsequently, the cell suspension is transferred to a polystyrene tube and placed within the magnetic field of the sorter. Under the influence of the magnetic field, the magnetically labeled non-target cells adhere to the tube wall, whereas the unlabeled CD8 T cells remain in the supernatant. This supernatant constitutes the T-cell‑enriched fraction after removal of the non-target cells.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e150 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eNegative Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8 °C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eHuman CD8 T lymphocytes are the core effector cells of adaptive cellular immunity in the human body, playing a crucial role in host anti-infectious immunity, tumor immune surveillance, and immune homeostasis regulation. Due to their precise antigen specificity, potent cytotoxic function, and immunological memory characteristics, human CD8 T cells are central research subjects in fields such as immunological mechanism studies, anti-infectious vaccine development, tumor immunotherapy (e.g., CAR-T cell therapy), and immune function assessment, possessing significant scientific research and clinical application value. This kit employs a column-based negative selection approach: non-CD8 T cells are labeled with biotin-conjugated antibodies, and magnetic bead separation is used to remove non-target cells, thereby enriching highly pure, high‑activity CD8 T cells. This method avoids the potential impact of direct labeling of CD8 T cells on cellular function and is suitable for subsequent experimental studies, including cell culture and functional assays.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cspan style=\"display:inline; white-space:nowrap;\"\u003eNote: This kit does not include sorting buffer; please provide it separately. The corresponding Stater product number is\u003ca href=\"https:\/\/www.starter-bio.com\/s0d3018_starter_magsep_separation_buffer.html\" style=\"color:#0066FF; display:inline; white-space:nowrap;\"\u003eS0D3018\u003c\/a\u003e\u003c\/span\u003e\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eSteps\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eOperating Instructions\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eDosage and timing\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003ePeripheral blood mononuclear cells (PBMCs) are isolated from peripheral blood by density-gradient centrifugation using lymphocyte separation medium, or cryopreserved PBMCs are directly thawed and resuspended, followed by cell counting.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eNote: It is recommended to count cells after staining with AO\/PI; PBMCs can be centrifuged at 400g for 7 minutes.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003ePrepare the sample\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eResuspend 1×10 in 100 μL of MagSep Separation Buffer.\u003c\/span\u003e\u003csup\u003e\u003cspan\u003eseven\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ecell;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eReagents are adjusted proportionally based on the sample volume; a pre-sorting sample is retained for assessing the proportion of human CD8 T cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003eseven\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ecells\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 10 μL of the Human CD8 T cell Biotin Antibody Cocktail to the sample and gently mix.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eIn this step, gently pipette up and down 2–3 times to mix thoroughly;\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10 μL\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate the antibodies in the cocktail with the cells at room temperature for 10 minutes;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate at room temperature for 10 minutes\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eShake for 5–30 seconds to mix the Streptavidin beads;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eShake for 5–30 seconds\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e6\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 10 μL of Streptavidin beads to the sample;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10 μL\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eGently mix the magnetic beads and cells, and incubate at room temperature for 5 minutes;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eFor mixing in this step, you can use a pipette to gently blow the mixture 2–3 times;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDuring this step, the magnetic beads may settle at the bottom; you can mix them again after about 2.5 minutes;\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate at room temperature for 5 minutes\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd MagSep Separation Buffer to the sample and immediately place it on the magnetic stand to prevent the magnetic beads from settling excessively;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd the total volume to 2.5 mL (5 mL flow cytometry tube)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd the total volume to 7.5 mL (15 mL centrifuge tube)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e9\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003ePlace the sample on the magnetic stand so that the magnetic beads adhere to the tube wall;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdsorb using a single-well magnetic stand (3 min)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdsorb using a multi-well magnetic stand (5 min)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTilt the magnetic stand and pour the sample into a new collection tube to collect the sample;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eThe sorted cells can be centrifuged at 400g for 7 minutes, suitable for subsequent culture and analysis.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCell sorting successful\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eFor 0.1–1 × 10^8 cells (0.1–1 mL), it is recommended to use a 5 mL single-well magnetic separator (Starter EasyIso Separator) with a total volume of 2.5 mL;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2–5 × 10^8 cells (2–5 mL) are recommended to be adsorbed using a 15 mL row-type magnetic separator (Starter EasyEights EasyIso Separator), with a total volume of 7.5 mL;\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"100T","offer_id":43030515908683,"sku":"S0K3008-100T","price":1000.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/cf74e860f1a54ea7bfc03c2f9dbbfe80.jpg?v=1788210032"},{"product_id":"human-cd8-t-cell-isolation-kit-column-based-s0k1011","title":"Human CD8 T Cell Isolation Kit (Column-Based)","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\n\u003cp\u003e\u003cspan\u003e1 mL Human CD8 T Cell Biotin-Antibody Cocktail\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1 mL Streptavidin Beads\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eCapacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eSuitable for a total cell count of 1×10⁹, and can perform up to 100 sorting runs.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePrinciple of Separation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eIn this negative selection process, non-CD8 T cells are labeled with biotin-conjugated antibodies, and the cells are then incubated with streptavidin‑nanomagnetic beads. As a result, the non‑target cells are marked by the antibody‑bead complex, while the target CD8 T cells remain unlabeled. Subsequently, the cell suspension is loaded onto a separation column; under the influence of a magnetic field, the magnetically labeled non‑target cells are retained within the column, whereas the unlabeled CD8 T cells elute into a collection tube under gravity. The resulting supernatant constitutes the CD8 T cell‑enriched fraction after removal of the non‑target cells.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMagnetic Bead Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e20 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSeparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eColumn-Based\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSelection Strategy\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eNegative Selection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore protected from light at 2–8°C; do not freeze.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eHuman CD8 T lymphocytes are the core effector cells of adaptive cellular immunity in the human body, playing a crucial role in host anti-infection immunity, tumor immune surveillance, and immune homeostasis regulation. Due to their precise antigen specificity, potent cytotoxic function, and immunological memory characteristics, human CD8 T cells are central research subjects in fields such as immunological mechanism studies, anti-infection vaccine development, tumor immunotherapy (e.g., CAR-T cell therapy), and immune function assessment, possessing significant scientific research and clinical application value. This kit employs a column-based negative selection approach: non-CD8 T cells are labeled with biotin-conjugated antibodies, and magnetic bead sorting is used to remove non-target cells, thereby enriching highly pure, high‑activity CD8 T cells. This method avoids the potential impact of direct labeling of CD8 T cells on cellular function and is suitable for subsequent experimental studies, including cell culture and functional assays.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cspan style=\"display:inline; white-space:nowrap;\"\u003eNote: This kit does not include the separation buffer; you need to prepare it yourself. The corresponding Stater product number is\u003ca href=\"https:\/\/www.starter-bio.com\/s0d3018_starter_magsep_separation_buffer.html\" style=\"color:#0066FF; display:inline; white-space:nowrap;\"\u003eS0D3018\u003c\/a\u003e\u003c\/span\u003e\u003ctable border=\"1\" style=\"border-collapse:collapse;width:auto;border:1px solid #000000\"\u003e\u003ctbody\u003e\u003ctr\u003e\u003ctd style=\"text-align:center;vertical-align:middle\"\u003e\n\u003cp\u003e\u003cspan\u003eTaking\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eL Separation Column\u003c\/span\u003e\u003cspan\u003eas an example\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eSteps\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eOperating instructions\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eDosage and time\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"6\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eCell processing and labeling\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIsolate PBMCs from peripheral blood using density gradient centrifugation with lymphocyte separation solution, or directly thaw cryopreserved PBMC cells, and then perform cell counting.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eNote: It is recommended to count cells after staining with AO\/PI; PBMCs can be centrifuged at 400 g for 7 minutes.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eSample preparation\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eResuspend 1×10^7 cells in 100 μL of MagSep Separation Buffer.\u003c\/span\u003e\u003csup\u003e\u003cspan\u003eNote:\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003eAdjust reagent volumes proportionally based on sample volume; set aside a pre-sorting sample to measure the proportion of human CD8 T cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e1×10^7\u003c\/span\u003e\u003cspan\u003ecells\/100 μL\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ecells\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 10 μL of Human CD8 T cell Biotin Antibody Cocktail to the sample and gently mix;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eIn this step, gently pipette the mixture 2–3 times to mix thoroughly;\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10 μL\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate the antibodies in the cocktail with the cells at room temperature for 10 min;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate at room temperature for 10 min\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eMix well\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eStreptavidin Beads\u003c\/span\u003e\u003cspan\u003e, add 10 μL\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eStreptavidin Beads\u003c\/span\u003e\u003cspan\u003einto the sample;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10 μL\/100 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e6\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eGently mix the magnetic beads and cells, and incubate at room temperature for 5 min;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIncubate at room temperature for 5 min\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eCell sorting\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eIn the column-based magnetic separator, install\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eL Separation Column\u003c\/span\u003e\u003cspan\u003e, add 3 mL of MagSep Separation Buffer to rinse the separation column;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eRinse the separation column\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd the cells to the separation column and collect the target cells using a collection tube;\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eThe cell volume should be at least 500 μL; if insufficient, add MagSep Separation Buffer to make up the volume. The cells that drip down under gravity are the target cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCollect the target cells\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e9\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAdd 3 mL of MagSep Separation Buffer and collect the target cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNote:\u003c\/span\u003e\u003cspan\u003eThis step elutes the unlabeled target cells from the separation column, increasing the yield.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eCollect the target cells\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"4\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eNotes: Pay attention to the maximum capacity of the separation column.\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eThe S Separation Column can hold a maximum of\u003c\/span\u003e\u003cspan\u003e1×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e7\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e;\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eThe L Separation Column can hold a maximum of\u003c\/span\u003e\u003cspan\u003e1×10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e8\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e;\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cbr\u003e\n\u003c\/td\u003e\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Starter","offers":[{"title":"100T","offer_id":43030516039755,"sku":"S0K1011-100T","price":1000.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/af31b4675238440196faae33e06abc7e.jpg?v=1788210022"}],"url":"https:\/\/www.antbioinc.com\/collections\/t-cell-detection-stimulation-kit.oembed?page=4","provider":"AntBio","version":"1.0","type":"link"}