{"title":"Other Kit","description":null,"products":[{"product_id":"kanamycin-residue-detection-kit","title":"Kanamycin residue detection kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eI. Preparation\u003cbr\u003e1, the kit\u003cbr\u003eThe kit components were allowed to equilibrate at room temperature for 30min before operation.\u003cbr\u003e2, need to bring your own supplies and equipment\u003cbr\u003e(1) enzyme standard instrument, thermostatic oscillator (or constant temperature incubator), washing machine, vortex generator, timer;\u003cbr\u003e(2) high-precision pipettor and disposable suction head (0.5-10uL, 10-100uL, 30-300uL, 100-1000uL);\u003cbr\u003e(3) deionized water (for preparation of lotion (1×));\u003cbr\u003e(4) the blotting paper, EP tubes, disposable gloves;\u003cbr\u003e3, the reagent preparation\u003cbr\u003e(1) Lotion (1×) was prepared\u003cbr\u003eApply lotion (1 x 20), plus 19 deionized water made into job concentration lotion (1 x). If there are crystals formed in the wash solution (20×), it should be placed at room temperature or 37℃ water bath with gentle shaking, and diluted after the crystals are completely dissolved. Lotion (20 x) should not use up at 2-8 ℃ storage.\u003cbr\u003e(2) Preparation of chromogenic solution\u003cbr\u003eTo color A and color liquid B volume mixing, avoid light place after blending. (note: the time is not too long, generally 10 min preparation before use. Do not use if the mixed chromogenic solution has turned blue.\u003cbr\u003e(3) Preparation of standard substance\u003cbr\u003eThe standard was diluted to 5ng\/mL with sample diluent, and then the standard was prepared by 2.5-fold dilution (each experiment used a newly prepared standard solution).\u003cbr\u003e4, sample preparation\u003cbr\u003eThe samples were returned to room temperature and mixed before adding the samples. If users need to dilute the high concentration of standard of form a complete set of product samples or box, the box sample diluent can be used for dilution; For cell samples, it is recommended to centrifuge at 3000rpm\/min for 5min before detection, and take the supernatant for detection.\u003cbr\u003e\u003cbr\u003e2, the operation process\u003cbr\u003e(1) will be resumed kit even at room temperature 30 min, from a balance to room temperature aluminum foil bag to take out the enzymes needed for test standard strip, with marker pen mark lath order (recommended for determination of complex pore), the remaining strip seal plate with film back again after sealing plate is aluminum foil bag, sealed and stored in 2-8 ℃. (Note: the slats are easy to fall off in the follow-up process, and pay attention to mark them.)\u003cbr\u003e(2) Sample incubation: Add 50uL standard\/blank (sample diluent)\/sample to each well, then add 50uL antibody working solution, seal plate with plate sealing membrane, and then place at 25℃ in the dark for 30min. (note: you must first add standard, if you add antibodies will directly and board antigen response; Incubate without seal plate or block in the process of incomplete, lead to the reaction liquid evaporation, cause the experiment error; All light) to avoid as far as possible in the process of incubation\u003cbr\u003e(3) Washing plate: After the incubation is completed, carefully remove the sealing plate membrane, discard the liquid in the hole, wash the plate 3 times (250uL\/ well) with wash solution (1×), and pat the residual liquid in the sample hole dry. (Note: if the hand washing plate is used, the washing solution (1×) needs to be suspended, and the tip of the gun is best not to touch the inner wall of the hole; After each join lotion (1 x) let stand for 30 s (at the end of the incubation process under mild concussion); Shoot every time when pay attention to in the new absorbent paper or dry clean area on the paper)\u003cbr\u003e(4) two resistance to incubate of enzyme mark: every hole to join enzyme mark two resistance, 100 ul\/hole, a sealing plate membrane sealing plate, and then placed in the 25 ℃ avoid light let stand incubate for 30 min.\u003cbr\u003e(5) Washing plate: the method is the same as step (3).\u003cbr\u003e(6) Color development: the pre-configured color development solution was added to the microplate according to 100uL\/ well, the plate was sealed with the plate sealing membrane, and the plate was left to incubate at 25℃ in the dark for 15min.\u003cbr\u003e(7) Termination: Add termination solution, 100uL\/ well, the color can be read after uniform. (Note: It is recommended to set 5-10s vibration in the reading program of the microplate reader)\u003cbr\u003eReading (8) : the enzyme label plate in the enzyme standard instrument, set the 450\/630 nm wavelength for the double wavelength, read the absorbance value, determination shall be completed within 20 min after termination.\u003cbr\u003e3. Data processing\u003cbr\u003e1, the absorbance value calculation\u003cbr\u003eThe absorbance values of each standard or sample ranged from OD450nm to OD630nm.\u003cbr\u003e2, however the computing of absorbance\u003cbr\u003eThe average absorbance value of each standard or sample (double well) divided by the average absorbance value of 0ng\/mL standard and multiplied by 100% is the percentage absorbance:\u003cbr\u003ePercentage of absorbance (%) = B\/B0 by 100%\u003cbr\u003eB: the average absorbance of the standard or sample values\u003cbr\u003eB0: Mean absorbance value of 0ng\/mL standard\u003cbr\u003e3. Drawing and calculation of the standard curve\u003cbr\u003eWith standard percentage absorbance value as the ordinate Y, standard chroma value is stere of abscissa X, drawing standard curve, it is recommended to use four parameter mathematical model fitting Logistic equation:\u003cbr\u003eY=((A-D)\/(1+(X\/C)^B))+D \u003cbr\u003eThe percentage absorbance value of the sample was substituted into the standard curve, the corresponding concentration value of the sample was read out, and the actual concentration of kanamycin in the sample was multiplied by its corresponding dilution.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis kit uses an indirect competitive ELISA method to determine trace residues of kanamycin in samples. The conjugated antigen is pre coated on the enzyme-linked plate, and the residual kanamycin in the sample competes with the conjugated antigen pre coated on the microporous strip for anti kanamycin antibodies. The enzyme-linked secondary antibody is added, and then the TMB substrate is added for color development. The absorbance (OD value) is measured using an enzyme-linked instrument at a wavelength of 450nm\/630nm, and the percentage absorbance is calculated. The concentration of kanamycin in the sample is negatively correlated with the percentage absorbance.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eKanamycin amino glucoside is a kind of antibiotics, used in the treatment of animal diseases, strain screening, and cell and gene therapy drug in the preparation of raw materials, since it has nerve toxicity and kidney toxicity, damage the eighth cranial nerve, cause the vestibular and cochlear damage. Residues in animal food and biological drugs can affect human health and even cause allergic reactions. European and American countries and our country require its limited use. \u003cbr\u003e        Kanamycin quantitatively detected by indirect competitive ELISA method, coupling in the process of the package is on the microporous article kanamycin antigen and the drug residues in the sample card competition combined with enzyme labeled kanamycin resistance of monoclonal antibody, then join enzyme mark 2, again by adding TMB chromogenic substrates, enzyme standard instrument were used to detect absorbance value, Absorbance values with the sample content of kanamycin in negative correlation. The operating time of this kit is only about one hour, and the linear range is 0.05ng\/ ml-5ng \/mL. \u003cbr\u003e        In the linear verification of kanamycin raw material, it is recommended to dilute kanamycin raw material to the linear range of the kit before determination, and dilute to at least 5 concentrations according to a certain proportion. The detection concentration is linearly related to the dilution. \u003cbr\u003e        Specificity should be validated against other substances present in the process. It is recommended to use high concentration and low concentration quality control samples, and add increasing concentrations of related interfering substances for specificity investigation. Did not join the analyte matrix should be measured at the same time. Quality control accuracy should be within a certain range, and did not join the analyte measured value of the matrix should be lower than the quantitative limit. \u003cbr\u003e\u003cbr\u003eProduct Features:\u003cbr\u003eStrong specificity: with penicillin, streptomycin, gentamicin sulfate and ammonia benzyl no cross reaction.\u003cbr\u003eHigh sensitivity: kit sensitivity of 0.05 ng\/mL.\u003cbr\u003eConvenient and efficient: use the two-step method, the results in a 1 hour or so.\u003cbr\u003eExcellent performance: standard curve and high accuracy, good repeatability.\u003cbr\u003e\u003cbr\u003eProduct performance index:\u003cbr\u003eDetection limit: \u0026lt; 0.05 ng\/mL\u003cbr\u003eLoq: 0.05 ng\/mL\u003cbr\u003eThe linear range was 0.05-5 ng\/mL\u003cbr\u003eAccuracy (recovery rate) : 70%-130%\u003cbr\u003eAccuracy (measurement deviation) : ≤15%\u003cbr\u003eDifference in repetitive (batch) : ≤15%\u003cbr\u003e\u003cbr\u003eSpecificity:\u003cbr\u003e\u003ctable style=\"border-collapse: collapse; width: 53.7451%; margin: 0 auto; page-break-inside: avoid;\" border=\"1\" cellspacing=\"0\" cellpadding=\"0\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 14.00pt;\"\u003e\n\u003ctd class=\"et2\" style=\"height: 14pt; width: 54.3563%; text-align: center;\" width=\"221\" height=\"28\"\u003eAntibiotics\u003c\/td\u003e\n\u003ctd class=\"et6\" style=\"width: 41.9315%; text-align: center;\" width=\"297\"\u003eCross-reactivity CR\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 14.00pt;\"\u003e\n\u003ctd class=\"et2\" style=\"height: 14pt; width: 54.3563%; text-align: center;\" height=\"28\"\u003eKanamycin\u003c\/td\u003e\n\u003ctd class=\"et7\" style=\"width: 41.9315%; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 14.00pt;\"\u003e\n\u003ctd class=\"et3\" style=\"height: 14pt; width: 54.3563%; text-align: center;\" height=\"28\"\u003eStreptomycin Sulfate\u003c\/td\u003e\n\u003ctd class=\"et6\" style=\"width: 41.9315%; text-align: center;\"\u003e＜1%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 14.00pt;\"\u003e\n\u003ctd class=\"et4\" style=\"height: 14pt; width: 54.3563%; text-align: center;\" height=\"28\"\u003eGentamicin\u003c\/td\u003e\n\u003ctd class=\"et6\" style=\"width: 41.9315%; text-align: center;\"\u003e＜1%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 14.00pt;\"\u003e\n\u003ctd class=\"et3\" style=\"height: 14pt; width: 54.3563%; text-align: center;\" height=\"28\"\u003ePenicillin\u003c\/td\u003e\n\u003ctd class=\"et6\" style=\"width: 41.9315%; text-align: center;\"\u003e＜1%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 14.00pt;\"\u003e\n\u003ctd class=\"et5\" style=\"height: 14pt; width: 54.3563%; text-align: center;\" height=\"28\"\u003eAmpicillin\u003c\/td\u003e\n\u003ctd class=\"et6\" style=\"width: 41.9315%; text-align: center;\"\u003e＜1%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"margin: 0 auto; page-break-inside: avoid; border-collapse: collapse; width: 74.6386%; height: 154px; border-color: #000000; border-style: solid;\" border=\"1\" cellspacing=\"0\" cellpadding=\"0\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 14.00pt;\"\u003e\n\u003ctd class=\"et2\" style=\"height: 14px; width: 12.0045%; text-align: center;\" width=\"96\" height=\"28\"\u003eNO.\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 38.3654%; text-align: center; height: 14px;\" width=\"252\"\u003eName\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 20.3951%; text-align: center; height: 14px;\" width=\"139\"\u003eSize\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 21.8981%; text-align: center; height: 14px;\" width=\"274\"\u003eStorage\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 14.00pt;\"\u003e\n\u003ctd class=\"et2\" style=\"height: 14px; text-align: center; width: 12.0045%;\" height=\"28\"\u003e1\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"text-align: center; width: 38.3654%; height: 14px;\"\u003eELISA plate\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"text-align: center; width: 20.3951%; height: 14px;\"\u003e8×12\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"text-align: center; width: 21.8981%; height: 14px;\"\u003eAvoid light at 2-8 ° C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 14.00pt;\"\u003e\n\u003ctd class=\"et2\" style=\"height: 14px; text-align: center; width: 12.0045%;\" height=\"28\"\u003e2\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"text-align: center; width: 38.3654%; height: 14px;\"\u003eStandard substance（50 ng\/mL）\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"text-align: center; width: 20.3951%; height: 14px;\"\u003e1mL\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"text-align: center; width: 21.8981%; height: 14px;\"\u003eAvoid light at 2-8 ° C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 14.00pt;\"\u003e\n\u003ctd class=\"et2\" style=\"height: 14px; text-align: center; width: 12.0045%;\" height=\"28\"\u003e3\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"text-align: center; width: 38.3654%; height: 14px;\"\u003eAntibody working solution\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"text-align: center; width: 20.3951%; height: 14px;\"\u003e7mL\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"text-align: center; width: 21.8981%; height: 14px;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 14.00pt;\"\u003e\n\u003ctd class=\"et2\" style=\"height: 14px; text-align: center; width: 12.0045%;\" height=\"28\"\u003e4\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"text-align: center; width: 38.3654%; height: 14px;\"\u003eEnzyme-labeled secondary antibody\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"text-align: center; width: 20.3951%; height: 14px;\"\u003e12mL\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"text-align: center; width: 21.8981%; height: 14px;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 14.00pt;\"\u003e\n\u003ctd class=\"et2\" style=\"height: 14px; text-align: center; width: 12.0045%;\" height=\"28\"\u003e5\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"text-align: center; width: 38.3654%; height: 14px;\"\u003esample dilution\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"text-align: center; width: 20.3951%; height: 14px;\"\u003e30mL\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"text-align: center; width: 21.8981%; height: 14px;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 14.00pt;\"\u003e\n\u003ctd class=\"et2\" style=\"height: 14px; text-align: center; width: 12.0045%;\" height=\"28\"\u003e6\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"text-align: center; width: 38.3654%; height: 14px;\"\u003ewashing liquor（20×）\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"text-align: center; width: 20.3951%; height: 14px;\"\u003e30mL\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"text-align: center; width: 21.8981%; height: 14px;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 14.00pt;\"\u003e\n\u003ctd class=\"et2\" style=\"height: 14px; text-align: center; width: 12.0045%;\" height=\"28\"\u003e7\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"text-align: center; width: 38.3654%; height: 14px;\"\u003eColor liquid A\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"text-align: center; width: 20.3951%; height: 14px;\"\u003e8mL\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"text-align: center; width: 21.8981%; height: 14px;\"\u003eAvoid light at 2-8 ° C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 14.00pt;\"\u003e\n\u003ctd class=\"et2\" style=\"height: 14px; text-align: center; width: 12.0045%;\" height=\"28\"\u003e8\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"text-align: center; width: 38.3654%; height: 14px;\"\u003eColor liquid B\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"text-align: center; width: 20.3951%; height: 14px;\"\u003e8mL\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"text-align: center; width: 21.8981%; height: 14px;\"\u003eAvoid light at 2-8 ° C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 14.00pt;\"\u003e\n\u003ctd class=\"et2\" style=\"height: 14px; text-align: center; width: 12.0045%;\" height=\"28\"\u003e9\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"text-align: center; width: 38.3654%; height: 14px;\"\u003estop buffer\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"text-align: center; width: 20.3951%; height: 14px;\"\u003e15mL\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"text-align: center; width: 21.8981%; height: 14px;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 14.00pt;\"\u003e\n\u003ctd class=\"et2\" style=\"height: 14px; text-align: center; width: 12.0045%;\" height=\"28\"\u003e10\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"text-align: center; width: 38.3654%; height: 14px;\"\u003esealing foil\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"text-align: center; width: 20.3951%; height: 14px;\"\u003e3\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"text-align: center; width: 21.8981%; height: 14px;\"\u003e-\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. The optimal reaction temperature for this reagent kit is 25 ℃. If the temperature is too high or too low, it will cause changes in the detection absorbance and sensitivity\u0026amp; Nbsp\u003call components in the reagent kit must be restored to room temperature before use nbsp\u003e\u003c\/all\u003e3. All components should be thoroughly mixed before use, and the standard sample needs to be briefly centrifuged for 5 seconds to concentrate all the liquid on the tube wall and lid at the bottom of the tube; Immediately return all reagents to 2-8 ℃ after use\u0026amp; Nbsp\u0026lt;4. The reagent kit must be used within its validity period, and corresponding standard curves must be prepared for each experiment. It is not recommended to use different batches of related reagents in mixed batches\u0026amp; Nbsp\u003cbr\u003e5. When adding liquid to the microplate, be careful not to touch the bottom of the microplate to prevent damage to the coating layer. Timely replace the sampling tank and suction head between different samples and steps to avoid cross contamination\u0026amp; Nbsp\u003cbr\u003e6. When the Flat noodles is dried after washing, pay attention to prevent the Flat noodles from falling off, and the sealing film should not be reused\u0026amp; Nbsp\u003cbr\u003e7. During color rendering, high concentrations may produce black flocculent matter, which is a normal phenomenon and does not affect the final reading result to a slight extent\u0026amp; Nbsp\u003cbr\u003e8. When reading, pay attention to checking whether the detection wavelength and fitting equation selection are correct\u0026amp; Nbsp\u003cbr\u003e9. Only by strictly following the operating methods in the manual and using all the reagents matched with this kit can the best detection effect be guaranteed\u0026amp; Nbsp\u003cthe difference in detection results can be caused by various factors including the operation of experimenter use pipette plate washing technology reaction time or temperature and storage reagent kit nbsp\u003e\u003c\/the\u003e11. Our company is only responsible for the reagent kit itself and is not responsible for sample consumption caused by the use of the reagent kit. Users are advised to fully consider the possible usage of the sample and reserve sufficient samples before use\u0026amp; Nbsp\u003cbr\u003e12. The termination solution in this reagent kit is an acid solution, and special attention should be paid during operation\u003cbr\u003e13. Operators should wear personal protective equipment, such as laboratory clothing, gloves, masks, and goggles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eThe kit should be protected from light. at 2-8 ℃, For 12 monthsNote that the kit that is not used up after opening is still protected from light at 2-8 ℃\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293314916427,"sku":null,"price":697.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_09652f08-3ea9-4f50-ae94-ae133cbc112b.png?v=1770714872"},{"product_id":"gentamicin-residue-detection-kit","title":"Gentamicin residue detection kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1, ready to work\u003c\/strong\u003e\u003cbr\u003e1. Kit preparation\u003cbr\u003ePlace the kit even at room temperature balance start operation after 30 min.\u003cbr\u003e2, need to bring their own consumables and equipment\u003cbr\u003e(1) Microplate reader, constant temperature oscillator (or constant temperature incubator), washing machine, vortex oscillator, timer;\u003cbr\u003e(2) high precision pipetting, and disposable suction (0.5-10 (including L, 10-100 (including L, including 30-300 L, 100-1000 (including L);\u003cbr\u003e(3) deionized water (for preparation of lotion (1×));\u003cbr\u003e(4) absorbent paper, EP tube, disposable gloves.\u003cbr\u003e3. Reagent preparation\u003cbr\u003e(1) Preparation of lotion (1×)\u003cbr\u003eOne part of the wash solution (20×) was taken and 19 parts of deionized water was added to make the working concentration wash solution (1×). If there are crystals formed in the wash solution (20×), it should be placed at room temperature or 37℃ water bath with gentle shaking, and diluted after the crystals are completely dissolved. Lotion (20 x) should not use up at 2-8 ℃ storage.\u003cbr\u003e(2) the color of liquid preparation\u003cbr\u003eAn equal volume of chromogenic solution A and B was mixed and placed in the dark after mixing. (note: the time is not too long, generally 10 min preparation before use. Such as color liquid mixture has turned blue, please do not use).\u003cbr\u003e(3) Preparation of standard substance\u003cbr\u003eThe standard dilution with sample diluent to 10 ng\/mL, then use 2.5 times than dilution method standard (each experiment using new preparation of standard solution)\u003cbr\u003e4, sample preparation\u003cbr\u003eThe samples were returned to room temperature and mixed before adding the samples. If users need to dilute the high concentration of standard of form a complete set of product samples or box, the box sample diluent can be used for dilution; For cell samples, it is recommended to centrifuge at 3000rpm\/min for 5min before detection, and take the supernatant for detection.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e2, the operation process\u003cbr\u003e\u003c\/strong\u003e(1) will be resumed kit even at room temperature 30 min, from a balance to room temperature aluminum foil bag to take out the enzymes needed for test standard strip, with marker pen mark lath order (recommended for determination of complex pore), the remaining strip seal plate with film back again after sealing plate is aluminum foil bag, sealed and stored in 2-8 ℃.\u003cbr\u003e(Note: the slats are easy to fall off in the follow-up process, and pay attention to mark them.)\u003cbr\u003e(2) Sample incubation: Add 50µL standard\/blank (sample diluent)\/sample to each well, then add 50µL antibody working solution, seal plate with plate sealing membrane, and then place at 37℃ in the dark for 30min.\u003cbr\u003e(Note: the standard must be added first, if the antibody is added first, it will directly react with the antigen on the plate; Incubate without seal plate or block in the process of incomplete, lead to the reaction liquid evaporation, cause the experiment error; Avoid light exposure during all incubations.)\u003cbr\u003e(3) Washing plate: After the incubation is completed, remove the sealing plate membrane carefully, discard the liquid in the hole, wash the plate 3 times (250µL\/ well) with wash solution (1×), and pat the residual liquid in the sample hole dry.\u003cbr\u003e(Note: if the hand washing plate is used, the washing solution (1×) needs to be suspended, and the tip of the gun is best not to touch the inner wall of the hole; After each addition of lotion (1×), it was left for 30s and slightly shaken. When patting dry, pay attention to each new absorbent paper or patting dry on a clean area of the paper)\u003cbr\u003e(4) two resistance to incubate of enzyme mark: every hole to join enzyme mark two resistance, including 100 L\/hole, a sealing plate membrane sealing plate, and then at 37 ℃ for incubate 30 min.\u003cbr\u003e(5) Washing plate: the method is the same as step (3).\u003cbr\u003e(6) color: preconfigured color liquid press, 100 (including L\/holes in enzyme label plate, seal plate membrane sealing plate, with 37 ℃ avoid light let stand incubate for 15 min.\u003cbr\u003e(7) termination: join terminated liquid, 100 (including L\/hole, reading after color evenly.\u003cbr\u003e(Note: It is recommended to set 5-10s vibration in the reading program of the microplate reader)\u003cbr\u003e(8) Reading: Put the microplate into the microplate reader, set the wavelength to double wavelength 450\/630nm, and read the absorbance value. The determination should be completed within 20min after termination.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e3. Data processing\u003c\/strong\u003e\u003cbr\u003e1. Calculation of absorbance value\u003cbr\u003eThe absorbance values of each standard or sample ranged from OD450nm to OD630nm.\u003cbr\u003e2. Calculation of percentage absorbance\u003cbr\u003eThe average absorbance value of each standard or sample (double well) divided by the average absorbance value of 0ng\/mL standard and multiplied by 100% is the percentage absorbance:\u003cbr\u003ePercentage of absorbance (%) = B\/B0 by 100%\u003cbr\u003eB: Mean absorbance value of standards or samples\u003cbr\u003eB0:0 ng\/mL the average absorbance of the standard value\u003cbr\u003e3. Drawing and calculation of the standard curve\u003cbr\u003eThe standard curve was drawn with the percentage absorbance value of the standard as the ordinate Y and the concentration value as the abscissa X. It is recommended to use the four-parameter Logistic mathematical model to fit the equation:\u003cbr\u003eY=((A-D)\/(1+(X\/C)^B))+D\u003cbr\u003eThe percentage absorbance value of the sample was substituted into the standard curve, the corresponding concentration value of the sample was read out, and the actual concentration of gentamicin in the sample was multiplied by its corresponding dilution.\u003cbr\u003e\u003cbr\u003e4. The four-parameter Logistic mathematical model is recommended for the fitting equation. ② Using the recommended double wavelength correction method as much as possible, using 630nm wavelength for correction, OD450-OD630 is the corrected OD value, which can be directly used for calculation, or according to the data quality, and then blank correction. If there is no dual-wavelength microplate reader, after reading OD450 data, the data quality should be judged before blank correction.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis kit uses an indirect competitive ELISA method to determine trace residues of gentamicin in the sample. The coupled antigen is pre coated on the micro well strip, and the residual gentamicin in the sample competes with the coupled antigen pre coated on the micro well strip for anti gentamicin antibodies. An enzyme-linked second antibody is added, and then the TMB substrate is added for color development. The absorbance (OD value) is measured using an enzyme-linked instrument at a wavelength of 450nm\/630nm, and the absorbance is negatively correlated with the content of gentamicin in the sample.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eGentamicin, a basic compound extracted from the fermentation broth of monospore genus of actinomycetes, is a commonly used aminoglycoside antibiotic and is widely used in the preparation of culture medium. It is mainly used to treat bacterial infections in clinic. Gentamicin has neurotoxicity, nephrotoxicity and ototoxicity. Its residues in animal food and biological drugs will affect human health and even cause allergic reactions. European and American countries and China require its limited use\u003cbr\u003e  The gentamicin quantitative detection kit adopts indirect competitive ELISA method. The pre coated gentamicin antigen on the micro well strip competes with the residual gentamicin in the sample to bind enzyme labeled gentamicin monoclonal antibody. Then the enzyme labeled secondary antibody is added, and then the TMB substrate is added to develop color. The absorbance value is detected by a microplate reader. The absorbance value is negatively correlated with the gentamicin content in the sample. The operation time of this kit is only about one hour, and the linear range is 0.1ng\/ml-10ng\/ml\u003cbr\u003e  When doing the linearity verification of gentamicin raw material, it is recommended to dilute the gentamicin raw material to the linear range of the kit and then determine it. Dilute it to at least 5 concentrations according to a certain proportion. The detection concentration is linearly related to the dilution multiple\u0026amp;Nbsp\u003cbr\u003e  Specificity should be verified for other substances present in the process. It is suggested to use high concentration and low concentration quality control samples and add increasing concentrations of relevant interfering substances for specificity investigation. The matrix without analytes should also be measured at the same time. The accuracy of the quality control sample should be within a certain range, and the measured value of the matrix without analytes should be lower than the lower limit of quantification.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eProduct Features: \u003c\/strong\u003e\u003cbr\u003eStrong Specificity: No cross reaction with penicillin, streptomycin sulfate, kanamycin and ampicil \u003cbr\u003eHigh Sensitivity: The sensitivity of the kit is 0.1ng\/ml \u003cbr\u003eConvenient and Efficient: Using two-step method, the result can be obtained in about 1 hour \u003cbr\u003eExcellent Performance: good linearity of standard curve, high accuracy, Good repeatability\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eProduct Performance Index: \u003c\/strong\u003e\u003cbr\u003edetection limit: \u0026lt;0.1 ng\/ml\u003cbr\u003elimit of quantification: 0.1 ng\/ml\u003cbr\u003elinear range: 0.1-10 ng \/ ml\u003cbr\u003eaccuracy (spiked recovery): 70% - 130% \u003cbr\u003eaccuracy (measurement deviation): ≤ 15%\u003cbr\u003erepeatability (intra batch difference): ≤ 15%\u003cbr\u003e\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003cstrong\u003eSpecificity: \u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e\u003ctable style=\"width: 43.5483%;\" border=\"1\" cellspacing=\"0\" cellpadding=\"0\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"et2\" style=\"text-align: center; width: 52%;\" width=\"221\" height=\"28\"\u003eAntibiotics\u003c\/td\u003e\n\u003ctd class=\"et6\" style=\"text-align: center; width: 39.3846%;\" width=\"297\"\u003eCross-reactivity CR\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"et2\" style=\"text-align: center; width: 52%;\" height=\"28\"\u003eGentamicin\u003c\/td\u003e\n\u003ctd class=\"et7\" style=\"text-align: center; width: 39.3846%;\"\u003e100%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"et3\" style=\"text-align: center; width: 52%;\" height=\"28\"\u003eStreptomycin Sulfate\u003c\/td\u003e\n\u003ctd class=\"et6\" style=\"text-align: center; width: 39.3846%;\"\u003e＜1%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"et4\" style=\"text-align: center; width: 52%;\" height=\"28\"\u003eKanamycin\u003c\/td\u003e\n\u003ctd class=\"et6\" style=\"text-align: center; width: 39.3846%;\"\u003e＜1%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"et3\" style=\"text-align: center; width: 52%;\" height=\"28\"\u003ePenicillin\u003c\/td\u003e\n\u003ctd class=\"et6\" style=\"text-align: center; width: 39.3846%;\"\u003e＜1%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"et5\" style=\"text-align: center; width: 52%;\" height=\"28\"\u003eAmpicillin\u003c\/td\u003e\n\u003ctd class=\"et6\" style=\"text-align: center; width: 39.3846%;\"\u003e＜1%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ctable style=\"margin: 0 auto; page-break-inside: avoid; border-collapse: collapse; width: 74.6386%; height: 154px; border-color: #000000; border-style: solid;\" border=\"1\" cellspacing=\"0\" cellpadding=\"0\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height: 14.00pt;\"\u003e\n\u003ctd class=\"et2\" style=\"height: 14px; width: 12.0045%; text-align: center;\" width=\"96\" height=\"28\"\u003eNO.\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 38.3654%; text-align: center; height: 14px;\" width=\"252\"\u003eName\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 20.3951%; text-align: center; height: 14px;\" width=\"139\"\u003eSize\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 21.8981%; text-align: center; height: 14px;\" width=\"274\"\u003eStorage\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 14.00pt;\"\u003e\n\u003ctd class=\"et2\" style=\"height: 14px; text-align: center; width: 12.0045%;\" height=\"28\"\u003e1\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"text-align: center; width: 38.3654%; height: 14px;\"\u003eELISA plate\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"text-align: center; width: 20.3951%; height: 14px;\"\u003e8×12\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"text-align: center; width: 21.8981%; height: 14px;\"\u003eAvoid light at 2-8 ° C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 14.00pt;\"\u003e\n\u003ctd class=\"et2\" style=\"height: 14px; text-align: center; width: 12.0045%;\" height=\"28\"\u003e2\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"text-align: center; width: 38.3654%; height: 14px;\"\u003eStandard substance（100 ng\/mL）\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"text-align: center; width: 20.3951%; height: 14px;\"\u003e1mL\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"text-align: center; width: 21.8981%; height: 14px;\"\u003eAvoid light at 2-8 ° C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 14.00pt;\"\u003e\n\u003ctd class=\"et2\" style=\"height: 14px; text-align: center; width: 12.0045%;\" height=\"28\"\u003e3\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"text-align: center; width: 38.3654%; height: 14px;\"\u003eAntibody working solution\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"text-align: center; width: 20.3951%; height: 14px;\"\u003e7mL\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"text-align: center; width: 21.8981%; height: 14px;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 14.00pt;\"\u003e\n\u003ctd class=\"et2\" style=\"height: 14px; text-align: center; width: 12.0045%;\" height=\"28\"\u003e4\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"text-align: center; width: 38.3654%; height: 14px;\"\u003eEnzyme-labeled secondary antibody\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"text-align: center; width: 20.3951%; height: 14px;\"\u003e12mL\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"text-align: center; width: 21.8981%; height: 14px;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 14.00pt;\"\u003e\n\u003ctd class=\"et2\" style=\"height: 14px; text-align: center; width: 12.0045%;\" height=\"28\"\u003e5\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"text-align: center; width: 38.3654%; height: 14px;\"\u003esample dilution\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"text-align: center; width: 20.3951%; height: 14px;\"\u003e30mL\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"text-align: center; width: 21.8981%; height: 14px;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 14.00pt;\"\u003e\n\u003ctd class=\"et2\" style=\"height: 14px; text-align: center; width: 12.0045%;\" height=\"28\"\u003e6\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"text-align: center; width: 38.3654%; height: 14px;\"\u003ewashing liquor（20×）\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"text-align: center; width: 20.3951%; height: 14px;\"\u003e30mL\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"text-align: center; width: 21.8981%; height: 14px;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 14.00pt;\"\u003e\n\u003ctd class=\"et2\" style=\"height: 14px; text-align: center; width: 12.0045%;\" height=\"28\"\u003e7\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"text-align: center; width: 38.3654%; height: 14px;\"\u003eColor liquid A\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"text-align: center; width: 20.3951%; height: 14px;\"\u003e8mL\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"text-align: center; width: 21.8981%; height: 14px;\"\u003eAvoid light at 2-8 ° C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 14.00pt;\"\u003e\n\u003ctd class=\"et2\" style=\"height: 14px; text-align: center; width: 12.0045%;\" height=\"28\"\u003e8\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"text-align: center; width: 38.3654%; height: 14px;\"\u003eColor liquid B\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"text-align: center; width: 20.3951%; height: 14px;\"\u003e8mL\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"text-align: center; width: 21.8981%; height: 14px;\"\u003eAvoid light at 2-8 ° C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 14.00pt;\"\u003e\n\u003ctd class=\"et2\" style=\"height: 14px; text-align: center; width: 12.0045%;\" height=\"28\"\u003e9\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"text-align: center; width: 38.3654%; height: 14px;\"\u003estop buffer\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"text-align: center; width: 20.3951%; height: 14px;\"\u003e15mL\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"text-align: center; width: 21.8981%; height: 14px;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 14.00pt;\"\u003e\n\u003ctd class=\"et2\" style=\"height: 14px; text-align: center; width: 12.0045%;\" height=\"28\"\u003e10\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"text-align: center; width: 38.3654%; height: 14px;\"\u003esealing foil\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"text-align: center; width: 20.3951%; height: 14px;\"\u003e3\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"text-align: center; width: 21.8981%; height: 14px;\"\u003e-\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. All components in the reagent kit must be restored to room temperature (20-25 ℃) before use\u0026amp; Nbsp\u003cbr\u003e2. All components should be thoroughly mixed before use, and the standard sample needs to be briefly centrifuged for 5 seconds to concentrate all the liquid on the tube wall and lid at the bottom of the tube; Immediately return all reagents to 2-8 ℃ after use\u0026amp; Nbsp\u0026lt;3. The reagent kit must be used within its validity period, and corresponding standard curves must be prepared for each experiment. It is not recommended to use different batches of related reagents in mixed batches\u0026amp; Nbsp\u0026lt;4. When adding liquid to the microplate, be careful not to touch the bottom of the microplate to prevent damage to the coating layer. Timely replace the sampling tank and suction head between different samples and steps to avoid cross contamination\u0026amp; Nbsp\u003cbr\u003e5. When the Flat noodles is dried after washing, pay attention to prevent the Flat noodles from falling off, and the sealing film should not be reused\u0026amp; Nbsp\u003cbr\u003e6. During color rendering, high concentrations may produce black flocs, which is a normal phenomenon and does not affect the final reading result to a slight extent\u0026amp; Nbsp\u003cwhen reading pay attention to checking whether the detection wavelength and fitting equation selection are correct nbsp\u003e\u003c\/when\u003e8. Only by strictly following the operating methods in the manual and using all the reagents matched with this reagent kit can the best detection effect be guaranteed\u0026amp; Nbsp\u003cthe difference in detection results can be caused by various factors including the operation of experimenter use pipette plate washing technology reaction time or temperature and storage reagent kit nbsp\u003e\u003c\/the\u003e10. Our company is only responsible for the reagent kit itself and is not responsible for sample consumption caused by the use of the reagent kit. Users are advised to fully consider the possible usage of the sample and reserve sufficient samples before use\u0026amp; Nbsp\u003cbr\u003e11. The termination solution in this reagent kit is an acid solution, and special attention should be paid during operation\u0026amp; Nbsp\u003cbr\u003e12. Operators should wear personal protective equipment, such as laboratory clothing, gloves, masks, and goggles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eThe kit should be protected from light. at 2-8 ℃, For 12 monthsNote that the kit that is not used up after opening is still protected from light at 2-8 ℃\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293314981963,"sku":null,"price":697.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_6d2cd223-f7a9-44e1-84d8-de8b28e1669e.png?v=1770714871"},{"product_id":"collagenase-type-i-residue-detection-kit","title":"Collagenase Type I Tesidue Detection Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\n\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e1. Preparation work:\u003c\/strong\u003e\u003cbr\u003e1. Kit preparation\u003cbr\u003eEach component of the kit was left to equilibrate at room temperature for 30 minutes before operation was started.  \u003cbr\u003e2. Required consumables and equipment (Note: You need to bring your own)\u003cbr\u003e(1) Microplate reader, constant temperature oscillator (or constant temperature incubator), plate washer (the plate washing method is hand washing or not), vortex oscillator, timer\u003cbr\u003e(2) High precision pipettes and disposable tips (0.5-10 µ L, 10-100 µ L, 30-300 µ L, 100-1000 µ L)\u003cbr\u003e(3) Deionized water\u003cbr\u003e(4) Absorbent paper, EP tube, disposable gloves\u003cbr\u003e3. Reagent preparation (Note: prepare according to the requirements according to the components provided in the kit)\u003cbr\u003e(1) Wash solution (1 ×): Take the wash solution (20 ×) and add deionized water to dilute it 20 times for later use. For example, take 10mL of wash solution (20 ×) and add 190mL of deionized water to mix well.\u003cbr\u003eNote: If crystals are formed in the wash solution (20 ×), shake gently in room temperature or 37 ℃ water bath, and then dilute after the crystals are completely dissolved)\u003cbr\u003e(2) Preparation of enzyme-labeled antibody solution: Dilute enzyme-labeled antibody (10 ×) 10 times with diluent to prepare enzyme-labeled antibody solution (1 ×). For example: 100μL enzyme-labeled antibody is added to 900μL diluent.\u003cbr\u003e(3) Preparation of color development solution: Mix color development solution A, color development solution B and other volumes, mix well and place it in the dark from light (Note: The time cannot be left for too long, generally prepare it 10min before use. If the color development solution has turned blue after mixing, please do not use it).  \u003cbr\u003e4. Preparation of standards\u003cbr\u003e(Note: It is prepared according to the requirements according to the standard provided by the kit. In order to further ensure the accuracy of the results, you can also use collagenase from specific sources actually used in the production process to establish the standard curve by yourself)\u003cbr\u003eDilute the standard (1 μg\/mL) to 20 ng\/mL with a diluent and then prepare the standard by serial dilution (dilution factor: 2 times) as shown in the following figure:\u003cbr\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/1_a4871f90-7bfb-4e45-897a-11f948fed3ce.png?v=1770873476\" alt=\"\"\u003e\u003cbr\u003eNote: In order to ensure the validity of the experimental results, a freshly prepared standard solution is used for each experiment.  \u003cbr\u003e5. Sample preparation\u003cbr\u003eReturn the sample to room temperature and mix well before adding the sample (the sample should be a homogeneous solution, and if there is precipitation, centrifuge the supernatant); If users need to dilute samples or high-concentration standards in the box, they can use the diluent in the box for dilution.\u003cbr\u003e\u003cstrong\u003e2. Operation process\u003c\/strong\u003e\u003cbr\u003e1. Detailed operation steps\u003cbr\u003eAll operations are performed at room temperature and repeat assays are recommended for all spiked wells.  \u003cbr\u003e(1) Return each component of the kit to room temperature for 30min, take out the slats required for the test from the aluminum foil bag that has been equilibrated to room temperature, mark the sequence of the slats with a marker, seal the remaining slats with a sealing film and put them back in the aluminum foil bag, seal them well, and store them at 2-8 ℃.  \u003cbr\u003e(Note: The slats are easy to fall off during the washing process, so be careful to mark them.)\u003cbr\u003e(2) Sample incubation: Add the diluted standard substance and the sample to be tested to the enzyme label plate (recommended addition sequence: standard well, blank well, sample well, standard substance is added according to the concentration gradient), 100 µ L\/well, seal the plate with a plate sealing membrane, and then place it at 37 ℃ for incubation for 1h.  \u003cbr\u003e(Note: The sample addition time should be controlled within 10min to avoid drift over time. If the plate is not sealed or the plate is incomplete during incubation, the reaction solution will evaporate, resulting in errors in the experiment.)\u003cbr\u003e(3) Plate washing: After incubation, carefully peel off the sealing film, discard the liquid in the wells, wash the plates three times (250 µ L\/well) with washing solution (1 ×), and pat dry the residual liquid in the sample wells. (If the plate washing method is hand washing, it can be allowed to stand for 1min after adding washing solution (1 ×); if the plate washing machine is used to wash the plates, it can be slightly shaken for 5s after adding washing solution.)\u003cbr\u003e(4) Incubation of enzyme-labeled antibody solution: Add enzyme-labeled antibody solution to each well, 100 µ L\/well, seal the plate with a plate sealing membrane, and then place it at 37 °C for 1h.  \u003cbr\u003e(Note: Before adding liquid after each plate washing, check whether the slats are fixed to prevent liquid splashing caused by fixing the slats after adding liquid.)\u003cbr\u003e(5) Plate washing: same as step 3).  \u003cbr\u003e(6) Color development: Add the pre-prepared color development solution to the enzyme labeled plate, 100 µ L\/well, seal the plate with a sealing membrane, and incubate at 37 °C in the dark for 20 minutes.  \u003cbr\u003e(7) Termination: Add the stop solution, 100 µ L\/well, and the reading can be made after the color is uniform (Note: Generally, the reading can be completed within 20 minutes after adding the stop solution).  \u003cbr\u003e(8) Reading: Put the microplate into the microplate reader, set the wavelength to dual wavelength 450\/630 nm, and read the absorbance value (Note: It is recommended to set a 5-10s shock in the microplate reader reading program).\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e3. Data processing:\u003c\/strong\u003e\u003cbr\u003e1. Calculation of absorbance value\u003cbr\u003eThe light absorption calibration values for each standard or sample are:\u003cbr\u003eStandard\/sample well absorbance values (OD\u003csub\u003e450nm\u003c\/sub\u003e-OD\u003csub\u003e630nm\u003c\/sub\u003e)-Blank control well absorbance value (OD\u003csub\u003e450nm\u003c\/sub\u003e-OD\u003csub\u003e630nm\u003c\/sub\u003e） \u003cbr\u003e2. Draw the standard curve with the standard concentration calibration value as the abscissa (X) and the standard light absorption calibration value as the ordinate (Y). It is recommended to use a four-parameter Logistic mathematical model to fit the equation:\u003cbr\u003eY = ((A-D)\/(1 + (X\/C) ^ B)) + D\u003cbr\u003eLight absorption calibration value [standard\/sample well absorbance value (OD\u003csub\u003e450nm\u003c\/sub\u003e-OD\u003csub\u003e630nm\u003c\/sub\u003e)-Blank control well absorbance value (OD\u003csub\u003e450nm\u003c\/sub\u003e-OD\u003csub\u003e630nm\u003c\/sub\u003e)] Substitute into the formula to calculate the content of collagenase in the sample.  \u003cbr\u003e3. If the OD value of the sample to be tested exceeds the OD value of the highest point of the standard curve, the sample needs to be diluted and re-measured\u003cbr\u003eThe following standard curve is for reference only, and the standard curve drawn by the same experimental standard shall prevail.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis kit uses double antibody sandwich enzyme-linked immunosorbent assay to determine the trace residue of Collagenase Type I (hereinafter referred to as collagenase) derived from Clostridium histolyticum in samples. Coating 96-well plates with capture antibodies to make solid phase antibodies, then adding standard and test samples, and then adding horseradish peroxidase (HRP) labeled labeled antibodies to form a solid phase antibody-collagenase-labeled antibody sandwich conjugate. Its absorbance (OD value) was measured at wavelengths of 450 nm and 630 nm, where 630 nm is the corrected wavelength. Results Calculate the standard\/sample well absorbance value (OD450nm-OD630nm)-blank control well absorbance value (OD450nm-OD630nm), and calculate the content of collagenase in the sample to be tested through the standard curve (it is recommended to use the four-parameter Logistic mathematical model fitting equation).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eCollagenase can be used to separate various tissues into single cells in vitro, and is suitable for single cell dissociation of organs and tissues for various scientific research purposes. The regulations related to the production of biological products clarify the necessity of detection of cell culture-related materials, so the detection of collagenase residues has attracted more and more attention.  \u003cbr\u003eThis product uses a double antibody sandwich enzyme-linked immunosorbent assay based on polyclonal antibody, developed for collagenase mixtures obtained from Clostridium histolyticum, which can quickly and effectively detect collagenase residues in various samples.\u003cbr\u003e\u003cstrong\u003eProduct Features:\u003cbr\u003e\u003c\/strong\u003eAccurate detection: Polyclonal antibodies prepared against collagenase mixtures can perform effective detection\u003cbr\u003eStrong specificity: no cross-reaction with other non-target proteins commonly used in cell culture\u003cbr\u003eExcellent performance: detection limit as low as 0.1 ng\/mL, high accuracy and good repeatability\u003cbr\u003eWide applicability: You can use collagenase from specific sources actually used in the production process to establish a standard curve by yourself to further ensure the accuracy of the results.\u003cbr\u003e\u003cstrong\u003eProduct performance indicators:\u003cbr\u003e\u003c\/strong\u003eLinearity range: 0.625-20ng\/mL\u003cbr\u003eLimit of quantitation: 0.625 ng\/mL\u003cbr\u003eLimit of detection: ≤ 0. 5 ng\/mL\u003cbr\u003eAccuracy (spiked recovery): 80%-120%\u003cbr\u003eAccuracy (measurement deviation): ≤ 15%\u003cbr\u003eRepeatability (intra-batch difference): ≤ 10%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable style=\"height: 254px; width: 69.1196%;\" border=\"1\" width=\"69.1196%\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 28px;\"\u003e\n\u003ctd class=\"et2\" style=\"width: 11.9693%; text-align: center; height: 28px;\" width=\"96\" height=\"28\"\u003eserial number\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 35.672%; text-align: center; height: 28px;\" width=\"252\"\u003eName\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 20.865%; text-align: center; height: 28px;\" width=\"139\"\u003eSpecifications\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 20.8591%; text-align: center; height: 28px;\" width=\"274\"\u003eSave\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 28px;\"\u003e\n\u003ctd class=\"et2\" style=\"width: 11.9693%; text-align: center; height: 28px;\" height=\"28\"\u003e1\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 35.672%; text-align: center; height: 28px;\"\u003eEnzyme plate\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 20.865%; text-align: center; height: 28px;\"\u003e8 × 12 bars\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 20.8591%; text-align: center; height: 28px;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd class=\"et2\" style=\"width: 11.9693%; text-align: center; height: 10px;\" height=\"28\"\u003e2\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 35.672%; text-align: center; height: 10px;\"\u003eStandard (1 µ g\/mL)\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 20.865%; text-align: center; height: 10px;\"\u003e\n\u003cp\u003e300 µ L\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 20.8591%; text-align: center; height: 10px;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 11.9693%; text-align: center; height: 22px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 35.672%; text-align: center; height: 22px;\"\u003eSample dilution\u003c\/td\u003e\n\u003ctd style=\"width: 20.865%; text-align: center; height: 22px;\"\u003e60mL\u003c\/td\u003e\n\u003ctd style=\"width: 20.8591%; text-align: center; height: 22px;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 11.9693%; text-align: center; height: 22px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 35.672%; text-align: center; height: 22px;\"\u003eEnzyme-labeled antibody (10 ×)\u003c\/td\u003e\n\u003ctd style=\"width: 20.865%; text-align: center; height: 22px;\"\u003e1.5 mL\u003c\/td\u003e\n\u003ctd style=\"width: 20.8591%; text-align: center; height: 22px;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 11.9693%; text-align: center; height: 22px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 35.672%; text-align: center; height: 22px;\"\u003eLotion (20 ×)\u003c\/td\u003e\n\u003ctd style=\"width: 20.865%; text-align: center; height: 22px;\"\u003e30mL\u003c\/td\u003e\n\u003ctd style=\"width: 20.8591%; text-align: center; height: 22px;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 28px;\"\u003e\n\u003ctd class=\"et2\" style=\"width: 11.9693%; text-align: center; height: 28px;\" height=\"28\"\u003e6\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 35.672%; text-align: center; height: 28px;\"\u003eChromogenic solution A\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 20.865%; text-align: center; height: 28px;\"\u003e8mL\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 20.8591%; text-align: center; height: 28px;\"\u003e2-8 ℃ protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 28px;\"\u003e\n\u003ctd class=\"et2\" style=\"width: 11.9693%; text-align: center; height: 28px;\" height=\"28\"\u003e7\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 35.672%; text-align: center; height: 28px;\"\u003eChromogenic solution B\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 20.865%; text-align: center; height: 28px;\"\u003e8mL\u003c\/td\u003e\n\u003ctd class=\"et2\" style=\"width: 20.8591%; text-align: center; height: 28px;\"\u003e2-8 ℃ protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 11.9693%; text-align: center; height: 22px;\"\u003e8\u003c\/td\u003e\n\u003ctd style=\"width: 35.672%; text-align: center; height: 22px;\"\u003eStop liquid\u003c\/td\u003e\n\u003ctd style=\"width: 20.865%; text-align: center; height: 22px;\"\u003e15mL\u003c\/td\u003e\n\u003ctd style=\"width: 20.8591%; text-align: center; height: 22px;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 11.9693%; text-align: center; height: 22px;\"\u003e9\u003c\/td\u003e\n\u003ctd style=\"width: 35.672%; text-align: center; height: 22px;\"\u003eSealing film\u003c\/td\u003e\n\u003ctd style=\"width: 20.865%; text-align: center; height: 22px;\"\u003e3 sheets\u003c\/td\u003e\n\u003ctd style=\"width: 20.8591%; text-align: center; height: 22px;\"\u003e-\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eCollagenase is a collagenase mixture obtained from Clostridium histolyticum, which can be used to separate a variety of tissues into single cells in vitro, and is suitable for single cell dissociation of organs and tissues for various scientific purposes. Collagenase is widely used in the isolation and acquisition of mesenchymal stem cells, 3D and organoid culture, single cell sequencing, tumor research and other fields. The bacterial collagenase commercially available on the market is mainly derived from Clostridium histolyticum. It is a crude enzyme extract that not only contains collagenase (clostridiopeptidase A), but also can degrade natural collagen and reticular fibers. It also contains some other proteases, polysaccharide enzymes, lipases, etc. According to the difference of collagenase activity, it can be divided into collagenase type I, type II, type III, type IV and type V, which can act on different types of tissue dissociation. Bacterial collagenases differ from vertebrate collagenases in that they have a wider substrate singularity. Unlike animal collagenase, which only decomposes the natural three-dimensional helical structure of collagen, bacterial collagenase is unique in that it can degrade both water-insoluble natural collagen and water-soluble denatured proteins. Bacterial collagenase can degrade almost all collagen types, and there are many kinds of divisions in the three-dimensional helical region. Collagenase is an endopeptidase, which can specifically hydrolyze the three-dimensional helical structure of natural collagen under physiological temperature and PH conditions. Collagen is the main fibrous component of animal extracellular connective tissue. This function of collagenase is mainly due to its ability to specifically recognize the Pro-X-Gly-Pro sequence and cleave the peptide bond between the neutral amino acid (X) and glycine (Gly) of this sequence, which is present in collagen with high frequency. Collagenase is also the only protease that can degrade natural collagen fibers with triple helices widely existing in connective tissue. Collagenase is also efficient enough to hydrolyze other proteins, polysaccharides and lipids within the extracellular matrix of connective tissue and epithelial tissue, making collagenase products well suited for tissue dissociation. In the treated biological products, there may be trace collagenase residues, which will have a certain impact on the subsequent application of biological products. The residue of collagenase in biological products is one of the important indexes to measure the quality of biological products.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. All components in the kit must be restored to room temperature (20-25 ℃) before use.  \u003cbr\u003e2. Each component should be thoroughly mixed before use to ensure the uniformity of the reagents. The standard product should be centrifuged briefly for 5 seconds. All the liquid on the tube wall and lid should be concentrated at the bottom of the tube. Immediately after use, all the reagents should be put back to 2-8 ℃.  \u003cbr\u003e3. The kit must be used within the validity period, and the corresponding standard curve must be re-prepared for each test. It is not recommended to mix different standard curves, and it is not recommended to mix different batches of related reagents.  \u003cbr\u003e4. When adding liquid to the microplate, be careful not to touch the bottom of the microplate to prevent damage to the coating layer. Change the sample loading tank and tip in time between different samples to avoid cross-contamination.\u003cbr\u003e5. When patting the slats dry after washing, be careful to prevent the slats from falling off, and be careful not to reuse the sealing film.  \u003cbr\u003e6. High concentration may produce black flocs during color development. It is recommended to dilute the sample for retest.  \u003cbr\u003e7. When reading, pay attention to check whether the detection wavelength and fitting equation are correct.  \u003cbr\u003e8. Only by strictly abiding by the operation methods of the instructions and using all the reagents supporting this kit can the best detection effect be guaranteed.  \u003cbr\u003e9. The kit uses accurate quantification of high-purity and high-activity collagenase as the standard product, which is suitable for most type I collagenases derived from Clostridium histolyticum. It can directly detect the residual levels of collagenase from different sources and can be used for more accurate detection of Merck Sigma-Aldrich collagenase. To further guarantee the accuracy of the results, it is also possible to self-establish standard curves using specific sources of collagenase actually used in the manufacturing process.  \u003cbr\u003e10. Differences in test results can be caused by many factors, including the operation of the experimenter, the use of the pipette, the plate washing technique, the reaction time or temperature, the storage of the kit, etc.  \u003cbr\u003e11. The company is only responsible for the kit itself, and is not responsible for the sample consumption caused by the use of the kit. Users are requested to fully consider the possible usage of samples and reserve sufficient samples before use.  \u003cbr\u003e12. The termination solution in this kit is acid solution, so special attention should be paid to operation.  \u003cbr\u003e13. For safety reasons, operators should wear personal protective equipment, such as lab coats, gloves, masks and goggles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eInstructions\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe Collagenase Type I quantitative detection kit (enzyme-linked immunosorbent assay) produced by our company is a highly sensitive collagenase detection kit, which is developed for the collagenase mixture obtained from Clostridium histolyticum, and can sensitively, specifically and accurately detect the residue of type I collagenase in intermediate products, semi-finished products and finished products of various tissue dissociation samples. The kit uses accurate quantification of high-purity and high-activity collagenase as the standard product, which is suitable for most type I collagenases derived from Clostridium histolyticum, and can directly detect the residual levels of collagenase from different sources. The standard can be used for more accurate detection of Merck Sigma-Aldrich collagenase type I. To further guarantee the accuracy of the results, it is also possible to self-establish standard curves using specific sources of collagenase actually used in the manufacturing process.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe kit should be stored at 2-8 ℃, protected from light, and the shelf life is 12 months. Note that the unused kit after opening should still be stored at 2-8 ℃ in the dark.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41293315047499,"sku":null,"price":1375.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_9e9c2489-84a4-4fa7-9647-0ca8b04fca19.png?v=1770714870"},{"product_id":"protein-a-elisa-kit","title":"Protein A Residue Detection Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003eI. self prepared materials: \u003c\/strong\u003e\u003cbr\u003e1, 10-1000ul pipette, single channel and multi-channel \u003cbr\u003e2, 100ml and 1000ml beakers \u003cbr\u003e3, deionized water \u003cbr\u003e4, various types of centrifuge tubes \u003cbr\u003e5, microplate reader (measuring 450nm) \u003cbr\u003e6, data processing and analysis software \u003cbr\u003e\u003cstrong\u003eII. Reagent preparation: \u003c\/strong\u003e\u003cbr\u003e1. Please place this kit at room temperature (20-25 ℃) before use\u003cbr\u003e2. Preparation of washing solution: add 270ml deionized water to 30ml of washing solution (10x) and mix well to prepare 300ml of working washing solution. If there are crystals in it, please dissolve and mix before use\u003cbr\u003e3. Other reagents can be used directly without dilution\u003cbr\u003e\u003cstrong\u003eIII. sample processing: \u003c\/strong\u003e\u003cbr\u003e1. If the sample needs to be diluted, use the sample diluent in the kit to dilute it, which can be carried out in a 96 well plate or EP tube\u003cbr\u003e2. Ensure that the volume of each hole (tube) containing the sample is 100ul\u003cbr\u003e3. Add 100ul of standard and quality control into the well\u003cbr\u003e4. Add 50ul acidification solution to each well, blow and mix well, and incubate at room temperature for 10min (acidify and separate proteinA and antibody in the sample)\u003cbr\u003e\u003cstrong\u003eIV. operation steps of the kit: \u003c\/strong\u003e\u003cbr\u003e1. Dilution of standard: mark 8 centrifuge tubes with 1#- 8#, add 990ul of sample diluent to 1#and 2#, and add 500ul of sample diluent to 3#- 8#. Take another 10ul of sample standard and add it to the 1 × tube, vortex and mix it well, then add 10ul to the 2 × tube and vortex and mix it well. Then add 500ul liquid from 2 × tube to 3 × tube for vortex mixing, and then take 500ul liquid from 3 × tube to 4 × tube for vortex mixing until 8 × tube\u003cbr\u003e\u003cbr\u003e\u003cimg src='%E2%80%9Chttps:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20230707\/fb96890650b64418860723922b09d80e.png\"Alt=\"'\u003e\u003cbr\u003eafter the dilution is complete, the concentration of protein a standard in the 2 × 8 × centrifuge tube is 5, 2.5, 1.25, 0.625, 0.3125, 0.156, 0.078ng\/ml respectively. Mark another tube as 0ng\/ml and add sample diluent. After the standard and sample are ready, enter the sample processing procedure. \u003cbr\u003e2. Add 100ul\/ well biotin label to the enzyme plate coated with protein A antibodyOf the binding antibody\u003cbr\u003e3. Add 25ul acidified standard, sample, quality control sample and blank into appropriate wells and incubate at room temperature for 1H\u003cbr\u003e4. After drying and washing for 4 times, 100ul\/ well of avidin labeled HRP was added and incubated for 10min at room temperature\u003cbr\u003e5. After drying and washing for 4 times, add 100ul\/ well TMB color developing solution for 10min, add 100ul\/ well stop solution, and then detect the absorbance at 450nm\u003cbr\u003e\u003cstrong\u003ev. data processing and result analysis: \u003c\/strong\u003e\u003cbr\u003euse software (such as curve expert or ELISA Calc) to establish the detection standard curve through the concentration gradient of the standard, and use 4-parameter logical fitting or cubic polynomial linear fitting to synthesize the corresponding relationship between the OD value and the concentration of the standard, Then the OD value of the test sample is brought into the corresponding curve equation to calculate the corresponding sample concentration\u003cbr\u003e\u003cstrong\u003eVI. indicators of analytical method: \u003c\/strong\u003e\u003cbr\u003e1. Precision: when the sample concentration is greater than 0.3ng\/ml, the coefficient of variation of detection is less than 10%; When the sample concentration is less than 0.3ng\/ml, the detection coefficient of variation is slightly greater than 10%\u003cbr\u003e2. It is suggested that when testing specific samples, the method of adding quality control materials should be used to investigate the influence of sample matrix effect on the test results\u003cbr\u003e3. The sensitivity of this kit is 0.05ng\/ml\u003cbr\u003e4. Hook effect: this kit is a double antibody sandwich one-step method, and the concentration of hook effect is 10ug\/ml. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTheory \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis reagent kit uses a dual antibody sandwich technique for labeling biotin systems. The enzyme-linked plate is pre coated with specific monoclonal antibodies against protein A. The sample containing protein A is diluted with sample diluent, and then the acidification solution is mixed to separate protein A from the antibody product. The sample reacts with the protein A pre coated on the enzyme-linked plate to capture antibodies. Then, the biotin labeled detection antibody reacts to form a sandwich complex. Then wash to remove substances that did not participate in the reaction, add HRP labeled with avidin, incubate briefly, perform TMB color development, and then add a termination solution to terminate the color development reaction. The absorbance value is detected at 450nm, and the OD value is directly proportional to the sample content.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis protein a detection ELISA kit is used to quantitatively detect non natural protein a such as recombinant alkali resistance. It can only be used in scientific research and production, and cannot be applied to the diagnosis and treatment of human or animal diseases. This kit provides a sample acidification method to separate protein A from IgG products. When the concentration of humanized monoclonal antibody is up to 2mg\/ml, the detection limit of protein A is lower than 78pg\/ml\u003cbr\u003eproduct components: \u003cbr\u003e1. Protein a standard \u003cbr\u003erecombinant alkali tolerant protein A in a protein matrix with conservative, 50ug\/ml, 1x01ml\u003cbr\u003e2. Biotinylated anti protein a monoclonal antibody in a protein matrix with preserved, 1x13ml\u003cbr\u003e3. Sample diluent \u003cbr\u003etris buffered saline with a protein matrix andpreserved, 1x30ml\u003cbr\u003e4Denaturing buffer (acidification buffer) \u003cbr\u003ecitrate buffer with reagent and preserved, 1x20ml\u003cbr\u003e5, 10x assay buffer (10x) \u003cbr\u003ewashing buffer with preserved, 1x30ml\u003cbr\u003e6, streptavidin labeled HRP in a protein matrix withprservative, 1x13ml\u003cbr\u003e7TMB substrate \u003cbr\u003e3,3’, 5,5’ Tetramethylbenzidine, 1x13ml\u003cbr\u003e8, stop solution \u003cbr\u003e2m sulphuric acid, 1x13ml\u003cbr\u003e9, monoclonal anti protein a coated plate of 96 wells \u003cbr\u003e12x8 well strips in a bag with destructive\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. This kit can only be used for scientific research and production, not for in vitro diagnosis\u003cbr\u003e2. The termination solution is 2m sulfuric acid, avoiding contact with eyes, skin and clothing. In addition, the reagents in this kit will not cause any harm to human body\u003cbr\u003e3. High or low pH value, detergent, urea, high salt concentration and organic reagent are all the influencing factors of ELISA method. PH has a great influence. The pH value of the sample should be controlled at 7.0-7.4\u003cbr\u003e4. If you have any questions during use, please contact our technical department. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2-8 ℃, 6months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41518198947915,"sku":null,"price":750.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/8be814e4d8e946379fb55cca64c0be96_0f9ef9d1-c013-481e-b12f-a2747e0ec552.png?v=1755257709"},{"product_id":"nad-nadh-assay-kit-with-wst-8","title":"NAD+\/NADH Assay Kit with WST-8","description":"\u003cdiv class=\"introduce_template\" data-v-60bf9651=\"\"\u003e\n\u003cdiv class=\"template_title\" data-v-60bf9651=\"\"\u003e\u003cstrong\u003eOverview\u003c\/strong\u003e\u003c\/div\u003e\n\u003cdiv class=\"template_item\" data-v-60bf9651=\"\"\u003e\n\u003cdiv class=\"label otherNameAttr\" data-v-60bf9651=\"\"\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/div\u003e\n\u003cdiv class=\"value\" data-v-60bf9651=\"\"\u003e\n\u003cdiv class=\"introduce_richText\" data-v-60bf9651=\"\"\u003eNAD+\/NADH Colorimetric Assay Kit (WST-8)\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"template_item\" data-v-60bf9651=\"\"\u003e\n\u003cdiv class=\"label\" data-v-60bf9651=\"\"\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/div\u003e\n\u003cdiv class=\"value\" data-v-60bf9651=\"\"\u003e\n\u003cdiv class=\"introduce_richText\" data-v-60bf9651=\"\"\u003e\n\u003cp\u003e\u003cstrong\u003eInspection principle:\u003c\/strong\u003e\u003cbr\u003eOxidized nicotinamide adenine dinucleotide (NAD +) and reduced nicotinamide adenine dinucleotide (NADH) are coenzymes that transfer electrons during redox reactions, and can be used as cofactors of many enzymes to participate in intracellular reactions.\u003cbr\u003eDetermination of total NAD + and NADH:\u003cbr\u003eEthanol produces acetaldehyde under the action of enzymes. During this reaction, NAD + is converted into NADH. NADH reduces WST-8 to produce an orange-yellow substance under the action of electron coupling reagent, with a maximum absorption peak around 450 nm. The amount of NAD + and NADH in the reaction sample of orange-yellow substance generated in the reaction system.\u003cbr\u003eThe amount of NADH was determined alone:\u003cbr\u003eAfter the sample is extracted and heated in a water bath at 60 °C for 30 minutes, the NAD + in the sample will decompose and only NADH will be retained. NADH reduces WST-8 to produce a yellow substance, and the amount of NADH in the sample can be determined separately.\u003cbr\u003eDetermination of NAD + and NAD +\/NADH ratios:\u003cbr\u003eAccording to the total amount of NAD + and NADH obtained in the first two steps and the amount of NADH alone, the amount of NAD + in the sample and the ratio of NAD + to NADH can be obtained. During the above assay, due to the specificity of the enzyme reagent, NADP + and NADPH have no influence on the assay results.\u003cbr\u003eWhen testing samples with this kit, it is necessary to measure the total protein concentration. It is recommended to use our company's BCA kit (abs9232) for measurement.\u003cbr\u003e\u003cstrong\u003eProduct composition:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eserial number\u003c\/td\u003e\n\u003ctd\u003eName\u003c\/td\u003e\n\u003ctd\u003eSpecifications\u003c\/td\u003e\n\u003ctd\u003eSave\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eReagent 1\u003c\/td\u003e\n\u003ctd\u003eExtract Extracting Solution\u003c\/td\u003e\n\u003ctd\u003e60 mL × 2\u003c\/td\u003e\n\u003ctd\u003e-20℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eReagent 2 (Reagent 2)\u003c\/td\u003e\n\u003ctd\u003eBuffer Buffer Solution\u003c\/td\u003e\n\u003ctd\u003e16 mL × 1\u003c\/td\u003e\n\u003ctd\u003e-20℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eReagent 3\u003c\/td\u003e\n\u003ctd\u003eColor developer (Chromogenic Agent)\u003c\/td\u003e\n\u003ctd\u003e5 mL × 1\u003c\/td\u003e\n\u003ctd\u003e-20 ℃, protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eReagent 4 (Reagent 4)\u003c\/td\u003e\n\u003ctd\u003eEnzyme reagent Enzyme Reagent\u003c\/td\u003e\n\u003ctd\u003ePowder × 2\u003c\/td\u003e\n\u003ctd\u003e-20 ℃, protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eReagent 5\u003c\/td\u003e\n\u003ctd\u003eStandard\u003c\/td\u003e\n\u003ctd\u003ePowder × 2\u003c\/td\u003e\n\u003ctd\u003e-20 ℃, protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e \u003c\/td\u003e\n\u003ctd\u003e96-well plate\u003c\/td\u003e\n\u003ctd\u003e1 plate\u003c\/td\u003e\n\u003ctd\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e \u003c\/td\u003e\n\u003ctd\u003e96-well coating\u003c\/td\u003e\n\u003ctd\u003e2 sheets\u003c\/td\u003e\n\u003ctd\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e \u003c\/td\u003e\n\u003ctd\u003eSample Location Marker Table\u003c\/td\u003e\n\u003ctd\u003e1 sheet\u003c\/td\u003e\n\u003ctd\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cbr\u003eNote: The reagents are stored strictly according to the storage conditions in the above table, and the reagents in different test kits cannot be mixed. For reagents with smaller volumes, please centrifuge before use, so as not to measure enough reagents. When this kit only measures the total amount of NAD + and NADH or the amount of NADH in a sample, one kit can measure 80 samples; When determining NAD + or the ratio of NAD + to NADH, one kit can determine 40 samples.\u003c\/div\u003e\n\u003ci class=\"el-icon-arrow-down introduce_icon\" data-v-60bf9651=\"\"\u003e\u003c\/i\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"template_item\" data-v-60bf9651=\"\"\u003e\n\u003cdiv class=\"label\" data-v-60bf9651=\"\"\u003eUsage\u003c\/div\u003e\n\u003cdiv class=\"value\" data-v-60bf9651=\"\"\u003e\n\u003cdiv class=\"introduce_richText\" data-v-60bf9651=\"\"\u003e\n\u003cstrong\u003e1. Required self-brought items:\u003c\/strong\u003e\u003cbr\u003eInstrument: microplate reader (450 nm), thermostatic water bath, 37 °C thermostatic box\u003cbr\u003eReagent: PBS (0.01 M, pH 7.4)\u003cbr\u003eConsumables: 10 KD ultrafiltration tube\u003cbr\u003e\u003cstrong\u003e2. Reagent preparation:\u003c\/strong\u003e\u003cbr\u003e(1) Before testing, all reagents are equilibrated to room temperature.\u003cbr\u003e(2) Preparation of reagent 4 working solution:\u003cbr\u003eEach reagent powder is fully dissolved in 200μL of double-distilled water, placed at 4 °C in the dark for 5 hours before use, and the unused part can be stored at 4 °C in the dark for 7 days.\u003cbr\u003e(3) Preparation of reaction working solution:\u003cbr\u003eMix the working solution of reagent 4 and reagent 2 at a volume ratio of 1:39, prepare it as needed before use, prepare it for use now, and store it in the dark from light. The prepared working solution is effective within 2 hours.\u003cbr\u003e(4) Preparation of 250μmol\/L standard stock solution:\u003cbr\u003eEach reagent 5 is fully dissolved with 200μL of double-distilled water, and the unused part can be stored in the dark for 7 days at-20 °C.\u003cbr\u003e(5) Preparation of 5μmol\/L standard solution:\u003cbr\u003eDilute according to the ratio of 250μmol\/L standard stock solution: reagent-volume ratio of 1:49 to obtain 5μmol\/L standard, which is prepared and used as needed before use, stored in the dark from light, and used within 1 day.\u003cbr\u003e(7) Dilution of different concentration standards:\u003cbr\u003e\n\u003ctable border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eserial number\u003c\/td\u003e\n\u003ctd\u003e（1）\u003c\/td\u003e\n\u003ctd\u003e（2）\u003c\/td\u003e\n\u003ctd\u003e（3）\u003c\/td\u003e\n\u003ctd\u003e（4）\u003c\/td\u003e\n\u003ctd\u003e（5）\u003c\/td\u003e\n\u003ctd\u003e（6）\u003c\/td\u003e\n\u003ctd\u003e（7）\u003c\/td\u003e\n\u003ctd\u003e（8）\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStandard Concentration (μmol\/L)\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003ctd\u003e1\u003c\/td\u003e\n\u003ctd\u003e1.5\u003c\/td\u003e\n\u003ctd\u003e2\u003c\/td\u003e\n\u003ctd\u003e2.5\u003c\/td\u003e\n\u003ctd\u003e3.5\u003c\/td\u003e\n\u003ctd\u003e4\u003c\/td\u003e\n\u003ctd\u003e5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e5 μmol\/L standard (μL)\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003ctd\u003e40\u003c\/td\u003e\n\u003ctd\u003e60\u003c\/td\u003e\n\u003ctd\u003e80\u003c\/td\u003e\n\u003ctd\u003e100\u003c\/td\u003e\n\u003ctd\u003e140\u003c\/td\u003e\n\u003ctd\u003e160\u003c\/td\u003e\n\u003ctd\u003e200\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eReagent One (μL)\u003c\/td\u003e\n\u003ctd\u003e200\u003c\/td\u003e\n\u003ctd\u003e160\u003c\/td\u003e\n\u003ctd\u003e140\u003c\/td\u003e\n\u003ctd\u003e120\u003c\/td\u003e\n\u003ctd\u003e100\u003c\/td\u003e\n\u003ctd\u003e60\u003c\/td\u003e\n\u003ctd\u003e40\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cstrong\u003e3. Sample preparation:\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e1) Sample processing\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003eSample homogenate:\u003c\/strong\u003e\u003cbr\u003eTissue sample: The homogenate medium is reagent 1, centrifuge at 10000 × g at 4 °C for 10 minutes, take the supernatant, and retain part of the supernatant for protein concentration determination.\u003cbr\u003eCell samples: After washing the cells with pre-cooled PBS (0.1 M, pH 7.4) on ice, take about 1.5 × 106 cells, add 0.4 mL of pre-cooled reagent-homogenate, centrifuge at 12000 × g for 10 minutes at 4 °C, take the supernatant, and retain part of the supernatant for protein concentration determination.\u003cbr\u003e\u003cstrong\u003eSample ultrafiltration:\u003cbr\u003e\u003c\/strong\u003eThe homogenates of tissues and cells contain enzymes that can decompose NAD +. It is recommended that after sample extraction and centrifugation, the supernatant should be centrifuged in a 10 KD ultrafiltration tube at 4 °C and 10000 × g for 10 minutes to remove the decomposing enzymes.\u003cbr\u003eWhen measuring the total amount of NAD + and NADH, the supernatant of the sample to be tested after filtration by ultrafiltration tube is taken for direct measurement.\u003cbr\u003eIf you want to measure the amount of NADH separately, take an appropriate amount of the sample to be measured and put the filtrate after ultrafiltration in an EP tube, take it in a water bath at 60 °C for 30 minutes, cool with running water and mix it well to be measured.\u003cbr\u003e\u003cstrong\u003e2) Sample dilution\u003c\/strong\u003e\u003cbr\u003eBefore formal testing, 2-3 samples with large expected differences need to be selected to dilute into different concentrations for pre-experiment. According to the results of pre-experiments, combined with the linear range of this kit: 0.02-5.0 μmol\/L, please refer to the following table for dilution (for reference only):\u003cbr\u003e\n\u003ctable border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample\u003c\/td\u003e\n\u003ctd\u003eDilution factor\u003c\/td\u003e\n\u003ctd\u003eSample\u003c\/td\u003e\n\u003ctd\u003eDilution factor\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e10% mouse muscle\u003c\/td\u003e\n\u003ctd\u003eNot diluted\u003c\/td\u003e\n\u003ctd\u003e293T cells\u003c\/td\u003e\n\u003ctd\u003eNot diluted\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e10% mouse kidney tissue\u003c\/td\u003e\n\u003ctd\u003eNot diluted\u003c\/td\u003e\n\u003ctd\u003eHela cells\u003c\/td\u003e\n\u003ctd\u003eNot diluted\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\nNote: The diluent is reagent I\u003cbr\u003e\u003cstrong\u003e4. Key points of the experiment:\u003c\/strong\u003e\u003cbr\u003e1. After each reagent powder is fully dissolved in 200μL of double-distilled water, it should be placed at 4 °C in the dark for 5 hours before use. Pay attention to preparation in advance.\u003cbr\u003e2. Try to use fresh samples for measurement. After freezing the samples, the measurement results will be reduced or the values will not be measured.\u003cbr\u003e3. The sample extract liquid is heated in a 60 °C water bath for 30 minutes to decompose NAD +. During this process, the EP tube must be sealed to prevent the liquid from volatilizing; After the heating is completed, due to the condensation of water vapor, it needs to be fully mixed before the next operation is carried out.\u003cbr\u003e\u003cstrong\u003e5. Operation steps:\u003cbr\u003e\u003c\/strong\u003e1. Measurement well: Take 20μL of the sample to be tested and add it to the measurement well corresponding to the enzyme plate.\u003cbr\u003eStandard wells: Take 20μL of different concentration standards and add them to the corresponding standard wells of the enzyme label plate.\u003cbr\u003e2. Take 120μL of the reaction working solution and add it to the measurement well and standard well in step ①.\u003cbr\u003e3. Add 40μL of reagent three to the measurement well and standard well in ②.\u003cbr\u003e4. After vibrating the plate for 5 s and accurately incubating in a 37 °C incubator for 30 min, the OD value of each well was measured at a wavelength of 450 nm with a microplate reader.\u003cbr\u003e\u003cstrong\u003e6. Operation table:\u003c\/strong\u003e\u003cbr\u003e\n\u003ctable border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eStandard hole\u003c\/td\u003e\n\u003ctd\u003eMeasurement well\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample to be tested (μL)\u003c\/td\u003e\n\u003ctd\u003e—\u003c\/td\u003e\n\u003ctd\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDifferent concentration standards (μL)\u003c\/td\u003e\n\u003ctd\u003e20\u003c\/td\u003e\n\u003ctd\u003e—\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eReaction working solution (μL)\u003c\/td\u003e\n\u003ctd\u003e120\u003c\/td\u003e\n\u003ctd\u003e120\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eReagent III (μL)\u003c\/td\u003e\n\u003ctd\u003e40\u003c\/td\u003e\n\u003ctd\u003e40\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd colspan=\"3\"\u003eVibrate the plate for 5 s, accurately incubate in a 37 °C incubator for 30 min, and measure the OD value of each well with a microplate reader at a wavelength of 450 nm.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cstrong\u003e7. Result calculation:\u003c\/strong\u003e\u003cbr\u003eStandard fit curve: y = ax + b\u003cbr\u003eFormula for calculating the total amount of NAD + and NADH in the sample:\u003cbr\u003eNADtotal (μmol\/gprot) = (ΔA-b) ÷ a × f ÷ Cpr\u003cbr\u003eFormula for calculating NADH content in the sample:\u003cbr\u003eNADH (μmol\/gprot) = (ΔA-b) ÷ a × f ÷ Cpr\u003cbr\u003eCalculation formula of NAD + content in sample:\u003cbr\u003eNAD + (μmol\/gprot) = NADtotal-NADH\u003cbr\u003eFormula for calculating the ratio of NAD + to NADH in the sample:\u003cbr\u003eNAD + ⁄ NADH = (NADtotal-NADH)\/NADH × 100%\u003cbr\u003eNotes:\u003cbr\u003ey: standard OD value − blank OD value (OD value when the standard concentration is 0)\u003cbr\u003ex: Concentration of standard\u003cbr\u003eA: slope of the standard curve\u003cbr\u003eb: Intercept of the standard curve\u003cbr\u003e∆ A: measurement well OD value − blank well OD value (OD value when the standard concentration is 0)\u003cbr\u003eCpr: supernatant protein concentration before ultrafiltration tube filtration (gprot\/L)\u003cbr\u003ef: Dilution factor before sample is added to test system\u003cbr\u003e\u003cstrong\u003e8. Standard curve (data is for reference only)\u003cbr\u003e\u003c\/strong\u003e1. Add 20μL of standard substances with different concentrations, and carry out the experiment according to the operation steps. The OD value of each tube is shown in the following table:\u003cbr\u003e\n\u003ctable border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eStandard Concentration (μmol\/L)\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003ctd\u003e1\u003c\/td\u003e\n\u003ctd\u003e1.5\u003c\/td\u003e\n\u003ctd\u003e2\u003c\/td\u003e\n\u003ctd\u003e2.5\u003c\/td\u003e\n\u003ctd\u003e3.5\u003c\/td\u003e\n\u003ctd\u003e4\u003c\/td\u003e\n\u003ctd\u003e5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"2\"\u003eOD Value\u003c\/td\u003e\n\u003ctd\u003e0.148\u003c\/td\u003e\n\u003ctd\u003e0.451\u003c\/td\u003e\n\u003ctd\u003e0.571\u003c\/td\u003e\n\u003ctd\u003e0.728\u003c\/td\u003e\n\u003ctd\u003e0.843\u003c\/td\u003e\n\u003ctd\u003e1.038\u003c\/td\u003e\n\u003ctd\u003e1.290\u003c\/td\u003e\n\u003ctd\u003e1.576\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e0.158\u003c\/td\u003e\n\u003ctd\u003e0.456\u003c\/td\u003e\n\u003ctd\u003e0.579\u003c\/td\u003e\n\u003ctd\u003e0.720\u003c\/td\u003e\n\u003ctd\u003e0.866\u003c\/td\u003e\n\u003ctd\u003e1.168\u003c\/td\u003e\n\u003ctd\u003e1.291\u003c\/td\u003e\n\u003ctd\u003e1.525\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAverage OD value\u003c\/td\u003e\n\u003ctd\u003e0.153\u003c\/td\u003e\n\u003ctd\u003e0.454\u003c\/td\u003e\n\u003ctd\u003e0.584\u003c\/td\u003e\n\u003ctd\u003e0.724\u003c\/td\u003e\n\u003ctd\u003e0.854\u003c\/td\u003e\n\u003ctd\u003e1.103\u003c\/td\u003e\n\u003ctd\u003e1.290\u003c\/td\u003e\n\u003ctd\u003e1.550\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAbsolute OD value\u003c\/td\u003e\n\u003ctd\u003e0.000\u003c\/td\u003e\n\u003ctd\u003e0.301\u003c\/td\u003e\n\u003ctd\u003e0.431\u003c\/td\u003e\n\u003ctd\u003e0.571\u003c\/td\u003e\n\u003ctd\u003e0.950\u003c\/td\u003e\n\u003ctd\u003e1.137\u003c\/td\u003e\n\u003ctd\u003e1.397\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n2. Draw the standard curve according to the data in the above table, as shown in the figure below:\u003cbr\u003e\u003cimg height=\"302\" width=\"402\" alt=\"\" src=\"https:\/\/absin.oss-cn-shanghai.aliyuncs.com\/absin-china\/20250227\/2909f3984b1043adb148ab7c51a23805.jpg\"\u003e\n\u003c\/div\u003e\n\u003ci class=\"el-icon-arrow-down introduce_icon\" data-v-60bf9651=\"\"\u003e\u003c\/i\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"introduce_template\" data-v-60bf9651=\"\"\u003e\n\u003cdiv class=\"template_title\" data-v-60bf9651=\"\"\u003e\u003cstrong\u003eProperties\u003c\/strong\u003e\u003c\/div\u003e\n\u003cdiv class=\"template_item\" data-v-60bf9651=\"\"\u003e\n\u003cdiv class=\"label\" data-v-60bf9651=\"\"\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/div\u003e\n\u003cdiv class=\"value\" data-v-60bf9651=\"\"\u003e\n\u003cdiv class=\"introduce_richText\" data-v-60bf9651=\"\"\u003e-20 ℃, protected from light, valid for 6 months.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"template_item\" data-v-60bf9651=\"\"\u003e\n\u003cdiv class=\"label\" data-v-60bf9651=\"\"\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/div\u003e\n\u003cdiv class=\"value\" data-v-60bf9651=\"\"\u003e\n\u003cdiv class=\"introduce_richText\" data-v-60bf9651=\"\"\u003eThis kit is suitable for detecting the respective content, ratio and total content of NAD + and NADH in animal tissue and cell samples.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Absin","offers":[{"title":"96T","offer_id":41583453012043,"sku":null,"price":219.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/absin_27db9ab6-aae8-427d-bc05-c84de7c2f650.jpg?v=1755258043"}],"url":"https:\/\/www.antbioinc.com\/collections\/other-kit.oembed","provider":"AntBio","version":"1.0","type":"link"}